FastQCFastQC Report
Sat 18 Jun 2016
SRR578563_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578563_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7813112
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108225213.851740510055405No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT231830.2967191562081793No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG97170.12436785751951335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCACTCGGCCAGCTTCT87390.11185043808408224No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA84580.1082539198209369No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT84260.10784435190484917No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC83920.10740918599400598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2391200.092.050165
GGTATCA2404900.091.7002268
AAGCAGT2408400.091.695941
GTATCAA2408950.091.565769
TGGTATC2408900.091.486877
GTGGTAT2424100.090.986466
GCAGTGG2425700.090.834163
CAGTGGT2427350.090.676954
AGCAGTG2437400.090.578822
TCAACGC2397500.046.4474112-13
AACGCAG2404450.046.3185314-15
CAACGCA2396800.046.316412-13
ATCAACG2397100.046.29931610-11
CGCAGAG2411050.046.17903516-17
ACGCAGA2406800.046.14511514-15
TATCAAC2417150.046.04389610-11
CAGAGTA2399950.045.9324118-19
AGAGTAC2363500.045.82354718-19
GAGTACT1339050.045.70985820-21
GCAGAGT2428900.045.61105716-17
Produced by FastQC (version 0.11.5)