##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578563_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7813112
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.036446680912803	31.0	31.0	34.0	28.0	34.0
2	31.387402356449005	33.0	31.0	34.0	28.0	34.0
3	31.515987227624535	34.0	31.0	34.0	28.0	34.0
4	34.899095648443286	37.0	35.0	37.0	32.0	37.0
5	34.91848036992174	37.0	35.0	37.0	32.0	37.0
6	34.965542539259644	37.0	35.0	37.0	32.0	37.0
7	34.88859893471385	37.0	35.0	37.0	32.0	37.0
8	34.93664214720076	37.0	35.0	37.0	32.0	37.0
9	36.44103412315093	39.0	37.0	39.0	32.0	39.0
10-11	36.32000379618262	39.0	37.0	39.0	32.0	39.0
12-13	36.39446900287619	39.0	37.0	39.0	32.0	39.0
14-15	37.6723276845385	40.0	38.0	41.0	32.0	41.0
16-17	37.63881069412547	40.0	38.0	41.0	32.0	41.0
18-19	37.56066795151535	40.0	38.0	41.0	32.0	41.0
20-21	37.47980561394743	40.0	38.0	41.0	31.5	41.0
22-23	37.20958543791514	39.5	37.5	41.0	31.5	41.0
24-25	37.17232570069391	40.0	37.0	41.0	31.0	41.0
26-27	37.07480175632962	40.0	37.0	41.0	30.0	41.0
28-29	36.92792545403164	40.0	37.0	41.0	30.0	41.0
30-31	36.69781656784134	40.0	36.5	41.0	30.0	41.0
32-33	36.39991126455118	40.0	36.0	41.0	29.5	41.0
34-35	36.236215671809134	39.0	36.0	41.0	28.5	41.0
36-37	35.945552233220255	39.0	35.0	40.5	27.5	41.0
38-39	35.00249542051874	38.0	35.0	40.0	26.5	41.0
40-41	34.77404125270443	37.5	34.5	40.0	26.0	41.0
42-43	34.47353672902679	37.0	34.0	40.0	24.5	41.0
44-45	34.195308988275094	37.0	33.5	40.0	24.0	41.0
46-47	34.04034333566446	37.0	33.0	40.0	23.5	41.0
48-49	34.0315653352979	37.0	33.0	40.0	23.0	41.0
50-51	33.08992965927021	36.5	32.0	39.5	19.5	40.5
52-53	32.59504631445191	37.0	33.0	39.5	10.0	40.5
54-55	32.09908798184385	38.0	31.5	40.0	2.0	41.0
56-57	31.524028773681984	37.0	31.0	40.0	2.0	41.0
58-59	31.21036918451956	37.0	31.0	40.0	2.0	41.0
60-61	30.932735317246188	36.5	30.5	40.0	2.0	41.0
62-63	30.592432183232496	36.0	30.0	40.0	2.0	41.0
64-65	30.294778828205715	35.5	29.0	39.0	2.0	41.0
66-67	29.95184684412562	35.0	29.0	39.0	2.0	41.0
68-69	29.614682408238867	35.0	29.0	38.5	2.0	40.5
70-71	29.318864301446084	35.0	29.0	37.5	2.0	40.0
72-73	28.99553974139882	35.0	28.0	37.0	2.0	39.5
74-75	28.636831009206063	35.0	28.0	36.5	2.0	39.0
76-77	28.26721490745301	35.0	27.0	36.0	2.0	39.0
78-79	27.937583257477943	35.0	26.0	36.0	2.0	38.5
80-81	27.622835113588543	34.0	26.0	35.0	2.0	37.0
82-83	27.363104867817075	34.0	26.0	35.0	2.0	37.0
84-85	27.10192718343216	34.0	25.5	35.0	2.0	36.0
86-87	26.85922222796755	34.0	25.0	35.0	2.0	36.0
88-89	26.60151140800234	34.0	24.5	35.0	2.0	36.0
90-91	26.365568930792236	34.0	24.0	35.0	2.0	35.0
92-93	26.083747423561828	34.0	21.5	35.0	2.0	35.0
94-95	25.79949551471936	34.0	19.5	35.0	2.0	35.0
96-97	25.52457311504046	34.0	17.5	35.0	2.0	35.0
98-99	25.218538144595904	34.0	4.5	35.0	2.0	35.0
100-101	24.387507436217476	33.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5581777327859889
1101	2	0.599390048204441
1101	3	0.4955530815553324
1101	4	0.5809691227165601
1101	5	0.5223890225824448
1101	6	0.5164649059744306
1101	7	0.44439373283630346
1101	8	0.4669232742453744
1101	9	0.6030131285321616
1101	10-11	0.6789711265516871
1101	12-13	0.716748960940599
1101	14-15	0.8623602466438172
1101	16-17	0.7966115051941856
1101	18-19	0.7945928040229191
1101	20-21	0.8547223451274917
1101	22-23	0.8479371746362574
1101	24-25	0.8145185172548111
1101	26-27	0.9040426913686304
1101	28-29	1.0029501689183675
1101	30-31	1.0688197679237703
1101	32-33	1.0826467089959095
1101	34-35	1.132503961111233
1101	36-37	1.1565436601331314
1101	38-39	1.1056228723777153
1101	40-41	1.081031792979843
1101	42-43	1.1513218322246175
1101	44-45	1.283543587530751
1101	46-47	1.1720015297130786
1101	48-49	1.1551451767735088
1101	50-51	1.267440643923905
1101	52-53	1.1341755836806513
1101	54-55	1.0309367430711802
1101	56-57	1.025055452298691
1101	58-59	1.0421921296089351
1101	60-61	0.9720582227632093
1101	62-63	0.9448315454377045
1101	64-65	1.0099416161420471
1101	66-67	1.0055739479417767
1101	68-69	0.9766410740708551
1101	70-71	1.0334150888970726
1101	72-73	1.0019746530308176
1101	74-75	1.0256887063267008
1101	76-77	0.9832298271607733
1101	78-79	0.9938550136147128
1101	80-81	0.9809443590933071
1101	82-83	1.0586403514749847
1101	84-85	0.999486265849221
1101	86-87	1.0241813551127308
1101	88-89	0.9937530946227291
1101	90-91	1.0156654852665419
1101	92-93	1.1067185496436203
1101	94-95	1.144771275434202
1101	96-97	1.1488043884328398
1101	98-99	1.134658551270178
1101	100-101	1.3144791650961878
1102	1	0.45517672272766774
1102	2	0.4091280569987319
1102	3	0.4712011705150907
1102	4	0.39893701149180316
1102	5	0.48215301392940546
1102	6	0.5193255137090631
1102	7	0.47463087039542273
1102	8	0.43404397829255714
1102	9	0.6184371906385024
1102	10-11	0.5620732742751215
1102	12-13	0.58962320680007
1102	14-15	0.6955495883833578
1102	16-17	0.7346655368043713
1102	18-19	0.8171380996224258
1102	20-21	0.8066594959240874
1102	22-23	0.8218760466522212
1102	24-25	0.8294663026938878
1102	26-27	0.9076369228237979
1102	28-29	0.9374882271464173
1102	30-31	0.9660699935540791
1102	32-33	1.0748573561818873
1102	34-35	1.0637403028226302
1102	36-37	1.1026817405524483
1102	38-39	1.1058700720100987
1102	40-41	1.1664875312099667
1102	42-43	1.1218980921832369
1102	44-45	1.207210871956356
1102	46-47	1.2915085777886546
1102	48-49	1.2262166396832086
1102	50-51	1.2245366170364704
1102	52-53	1.0429722524150051
1102	54-55	1.0123037849293652
1102	56-57	0.9406765039115577
1102	58-59	0.9895656486059679
1102	60-61	0.9775944780241304
1102	62-63	0.9951474833818601
1102	64-65	0.9980561905786232
1102	66-67	0.9980110659101591
1102	68-69	0.9297775636539285
1102	70-71	0.8829577594429523
1102	72-73	0.9034386601708313
1102	74-75	0.7938551728142329
1102	76-77	0.9236636757121026
1102	78-79	0.9374239768022292
1102	80-81	0.9413544410895369
1102	82-83	0.9766131548977519
1102	84-85	0.9530900346903373
1102	86-87	0.9943236575112415
1102	88-89	1.0635015836803525
1102	90-91	1.082334972325782
1102	92-93	1.0629687751020853
1102	94-95	1.1533028528222253
1102	96-97	1.2019779048663572
1102	98-99	1.2555289139324337
1102	100-101	1.30704737998742
1103	1	0.4378898965034992
1103	2	0.46237303314811484
1103	3	0.39375566287098707
1103	4	0.4975960751744424
1103	5	0.4772104948032734
1103	6	0.502090250978128
1103	7	0.4302408514787217
1103	8	0.45517437056798116
1103	9	0.5693377727635394
1103	10-11	0.5579505064596404
1103	12-13	0.5846526091978959
1103	14-15	0.6735953072754342
1103	16-17	0.7673634068626711
1103	18-19	0.7414045651484358
1103	20-21	0.7315751667518811
1103	22-23	0.8242473388296929
1103	24-25	0.7506336258672377
1103	26-27	0.8152465903758284
1103	28-29	0.8508209396384174
1103	30-31	0.8949956943143533
1103	32-33	0.9583417346293785
1103	34-35	0.942440148579557
1103	36-37	1.0028888872244863
1103	38-39	0.8781005614640165
1103	40-41	1.0048082742901485
1103	42-43	0.8329429084904731
1103	44-45	0.7939614851490333
1103	46-47	1.0598692571160484
1103	48-49	1.099972428059239
1103	50-51	1.016880665486127
1103	52-53	0.8934540585255348
1103	54-55	0.7816317282350056
1103	56-57	0.8490212910643997
1103	58-59	0.8633989100546557
1103	60-61	0.9128972717589932
1103	62-63	0.784019554444626
1103	64-65	0.708368682562444
1103	66-67	0.6624225040658835
1103	68-69	0.815742397104394
1103	70-71	0.7521856373578508
1103	72-73	0.8513737193152373
1103	74-75	0.8281705475982903
1103	76-77	0.8224813330361549
1103	78-79	0.850123032722859
1103	80-81	0.8712993911695435
1103	82-83	0.9264968121295922
1103	84-85	0.9175379115876829
1103	86-87	0.9247342108934973
1103	88-89	0.9427129034117634
1103	90-91	0.895609418624371
1103	92-93	0.9499865865584098
1103	94-95	0.9389991308507426
1103	96-97	0.9232151099741834
1103	98-99	1.0031301504847399
1103	100-101	1.1094209859387014
1104	1	0.36047669393795445
1104	2	0.37176441963841356
1104	3	0.3818294001874776
1104	4	0.3449397855959191
1104	5	0.4133872884224985
1104	6	0.40299990953850795
1104	7	0.4116256250042625
1104	8	0.2960655907510201
1104	9	0.4973258300353649
1104	10-11	0.4612313322491133
1104	12-13	0.5270713239443481
1104	14-15	0.6048958572873246
1104	16-17	0.6064871229384252
1104	18-19	0.6988356286011381
1104	20-21	0.7243596624542477
1104	22-23	0.7152331756972217
1104	24-25	0.6964104955598458
1104	26-27	0.7275094042549597
1104	28-29	0.7571506904947753
1104	30-31	0.8450948673054555
1104	32-33	0.8523147984717028
1104	34-35	0.8975182782912938
1104	36-37	0.8899505750721275
1104	38-39	0.8896877366216671
1104	40-41	0.8183528974269976
1104	42-43	0.9478177459629293
1104	44-45	0.8917244377414661
1104	46-47	1.01142057831278
1104	48-49	0.9149873544040403
1104	50-51	0.8415177637194375
1104	52-53	0.7492291916167844
1104	54-55	0.6869774986192994
1104	56-57	0.6907548166080524
1104	58-59	0.694040402500768
1104	60-61	0.7031140098532376
1104	62-63	0.5848974097369926
1104	64-65	0.5009267467624952
1104	66-67	0.5848984258649566
1104	68-69	0.5541571964839775
1104	70-71	0.5192168801547155
1104	72-73	0.5293454226270562
1104	74-75	0.6364833823711642
1104	76-77	0.6675660737298443
1104	78-79	0.5307927346140033
1104	80-81	0.673512938279444
1104	82-83	0.6970371339147192
1104	84-85	0.7072605692453919
1104	86-87	0.7179659640627918
1104	88-89	0.7330258113829622
1104	90-91	0.7597652766962888
1104	92-93	0.6456287085431711
1104	94-95	0.4688275931356749
1104	96-97	0.6128166268814255
1104	98-99	0.8317125723777501
1104	100-101	0.7867903562563008
1105	1	0.23611584352067894
1105	2	0.16579375904329297
1105	3	0.1807738287864744
1105	4	0.17442476225333792
1105	5	0.1738478588699408
1105	6	0.11295169149607887
1105	7	0.09504568617209941
1105	8	0.1336983325986978
1105	9	0.0944351967611965
1105	10-11	0.10508941875416866
1105	12-13	0.09281532486748034
1105	14-15	-0.017331945493502587
1105	16-17	-0.01424202500430738
1105	18-19	-0.13429438370287272
1105	20-21	-0.047916531944380836
1105	22-23	-0.12271642390439297
1105	24-25	-0.12173323219155918
1105	26-27	-0.4140352430175085
1105	28-29	-0.19642003850983514
1105	30-31	-0.37557496180375693
1105	32-33	-0.34662069225549175
1105	34-35	-0.27454939805483747
1105	36-37	-0.1926622432520375
1105	38-39	-0.1273677727521303
1105	40-41	-0.10748028170461765
1105	42-43	-0.023804975734257994
1105	44-45	-0.02436435838068718
1105	46-47	0.013732938605862444
1105	48-49	0.006586955136569372
1105	50-51	0.011755176716754079
1105	52-53	-0.040441866191883946
1105	54-55	-0.08470426705747514
1105	56-57	0.07066281787846052
1105	58-59	0.11020021568074156
1105	60-61	0.053695722839954385
1105	62-63	0.08885597575110538
1105	64-65	0.02136814941660603
1105	66-67	0.029488637398213058
1105	68-69	0.05395168213603796
1105	70-71	0.04339716650304837
1105	72-73	0.04281169406479535
1105	74-75	0.09176148587841126
1105	76-77	0.12079234933087335
1105	78-79	0.12519439635094187
1105	80-81	0.2078002061255262
1105	82-83	0.17040339033866303
1105	84-85	0.08476115329030876
1105	86-87	0.14946860404661066
1105	88-89	0.2531503720340744
1105	90-91	0.28819437165915573
1105	92-93	0.3414934587045728
1105	94-95	0.2891019350030568
1105	96-97	0.32106072325526114
1105	98-99	0.35151277388899516
1105	100-101	0.3192934440708832
1106	1	-0.13249725382250332
1106	2	-0.12922317207671696
1106	3	-0.17003111220902412
1106	4	-0.1474742269763354
1106	5	-0.16334481170986948
1106	6	-0.19432328480544214
1106	7	-0.2176980055068114
1106	8	-0.23321441108729601
1106	9	-0.20295495086824644
1106	10-11	-0.3342814620475494
1106	12-13	-0.3723598011906333
1106	14-15	-0.3752033393370624
1106	16-17	-0.3811536636093109
1106	18-19	-0.42407179537548956
1106	20-21	-0.194889404544071
1106	22-23	-0.16611017404100892
1106	24-25	-0.31523749255949696
1106	26-27	-0.2965363622407011
1106	28-29	-0.29055484565592593
1106	30-31	-0.3149841686620434
1106	32-33	-0.2810451701249761
1106	34-35	-0.25564878741905517
1106	36-37	-0.3889417386454781
1106	38-39	-0.3666042963142928
1106	40-41	-0.31708913789618265
1106	42-43	-0.4429710779923468
1106	44-45	-0.3833223311058376
1106	46-47	-0.41261138957979426
1106	48-49	-0.6189184648486403
1106	50-51	-0.7449766817690957
1106	52-53	-0.8168593763231691
1106	54-55	-0.7867636060976579
1106	56-57	-0.8043159652771301
1106	58-59	-0.7276886044017559
1106	60-61	-0.9082615281784712
1106	62-63	-0.7398207346022865
1106	64-65	-0.7597181253722809
1106	66-67	-0.9421701534166225
1106	68-69	-1.0738310272877563
1106	70-71	-0.9499318290853509
1106	72-73	-1.0304754705480974
1106	74-75	-1.0641608069723425
1106	76-77	-1.0874318066196338
1106	78-79	-0.9811353668672282
1106	80-81	-0.9569440797671547
1106	82-83	-1.082645078627408
1106	84-85	-1.125367714574896
1106	86-87	-1.1121728787836176
1106	88-89	-1.1916445465377343
1106	90-91	-1.1387834468708853
1106	92-93	-1.1050803115847536
1106	94-95	-1.1026007441567245
1106	96-97	-1.0677030036628672
1106	98-99	-1.1653360335772405
1106	100-101	-1.3297859634106963
1107	1	-0.35043934623736916
1107	2	-0.4105872977126417
1107	3	-0.3592111900634478
1107	4	-0.24385674436118876
1107	5	-0.35854427348174056
1107	6	-0.3273478031700563
1107	7	-0.27729934029147074
1107	8	-0.2957461272293642
1107	9	-0.327988376935501
1107	10-11	-0.37554147045198505
1107	12-13	-0.41589660036414244
1107	14-15	-0.6245316156077294
1107	16-17	-0.46545344964465585
1107	18-19	-0.4805735346557256
1107	20-21	-0.35208370204343
1107	22-23	-0.45204677158221074
1107	24-25	-0.4639345582622667
1107	26-27	-0.37537067570202964
1107	28-29	-0.43504222395549874
1107	30-31	-0.41580769329666367
1107	32-33	-0.35429751822244526
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2308	48-49	-2.5358118284605062
2308	50-51	-2.44845385086213
2308	52-53	-2.388889272084807
2308	54-55	-2.1265648833539643
2308	56-57	-2.01821405797077
2308	58-59	-1.8785112242873332
2308	60-61	-1.871204792453316
2308	62-63	-1.9669530005243807
2308	64-65	-1.9290622414934901
2308	66-67	-2.088496849554897
2308	68-69	-1.9307210861766535
2308	70-71	-1.8697096349300821
2308	72-73	-1.8141921370891758
2308	74-75	-1.6786910533847355
2308	76-77	-1.5727813443302772
2308	78-79	-1.6916801840709184
2308	80-81	-2.2997305869320925
2308	82-83	-2.124386158633694
2308	84-85	-1.841118462620301
2308	86-87	-1.7347962099806544
2308	88-89	-1.6340653945569734
2308	90-91	-1.8181541133959804
2308	92-93	-1.884222910053868
2308	94-95	-1.97543817050229
2308	96-97	-1.9285995708520538
2308	98-99	-1.9694280792489742
2308	100-101	-2.1481783976121314
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78910.0
3	12774.0
4	11311.0
5	10250.0
6	11891.0
7	14662.0
8	17673.0
9	20926.0
10	23357.0
11	28770.0
12	28333.0
13	31486.0
14	34877.0
15	41082.0
16	52717.0
17	72454.0
18	113349.0
19	191191.0
20	278351.0
21	196906.0
22	101886.0
23	84401.0
24	85616.0
25	89631.0
26	97335.0
27	107821.0
28	123310.0
29	143132.0
30	167583.0
31	200965.0
32	246400.0
33	310601.0
34	408992.0
35	601190.0
36	1012693.0
37	1335352.0
38	1202774.0
39	221926.0
40	234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.320061487538453	49.76332624840218	23.09707619173724	16.81953607232213
2	16.014672134896323	51.3191252113648	17.322542918953904	15.343659734784968
3	46.53807749040385	19.652264807023364	18.14270516357171	15.666952539001077
4	16.008380305002635	18.096003411542256	19.063119294486913	46.832496988968195
5	16.01855864472037	48.84797567735863	20.04102024636487	15.092445431556126
6	46.73662848085453	19.177555323009376	18.036102282547706	16.049713913588388
7	16.809835455832232	18.62117928759859	49.61445859927232	14.95452665729686
8	46.327391972987726	19.365024712825004	18.913019687702594	15.394563626484672
9	45.992666800214785	19.013685268895724	19.409926379999014	15.583721550890473
10-11	15.647470779666293	34.15911155234879	34.30677484726782	15.886642820717098
12-13	15.465442044827801	19.367890467101386	34.2202620603517	30.946405427719114
14-15	15.62938835081884	49.57641104209469	18.865019552991996	15.92918105409447
16-17	30.843064817807807	19.16973756365913	18.981711185313653	31.005486433219403
18-19	15.818388127532737	34.22692377521073	18.9781261997127	30.976561897543842
20-21	31.025837473917235	34.26482338848722	19.004585754531263	15.704753383064283
22-23	30.91932408799184	19.086646662625583	34.23430658914887	15.759722660233699
24-25	15.74236124763473	34.04003503878636	19.447684747171916	30.769918966407
26-27	15.936322596408784	25.33749169813876	42.716632661406805	16.009553044045653
28-29	28.627364648662486	18.9881351593498	36.515954178932546	15.868546013055166
30-31	26.35133933011287	20.202037720653767	36.92042327226947	16.526199676963895
32-33	19.616723391881127	23.05059551235975	38.52314649849791	18.809534597261216
34-35	19.049315868185968	22.051006078832252	39.361729972930135	19.537948080051642
36-37	18.687866300290114	22.11350795735004	39.49969992669995	19.698925815659894
38-39	19.12628207474745	22.13838817282446	39.20896688844455	19.52636286398354
40-41	19.45601386021734	21.827128833477143	39.038274630258854	19.678582676046666
42-43	19.814051440537533	22.335726475072832	38.8184352761883	19.031786808201336
44-45	19.30351153462108	22.414659137313006	38.81312655064776	19.46870277741816
46-47	19.527003240546296	22.094776363780298	38.841259436394395	19.53696095927901
48-49	19.306016685140293	22.066603608884748	39.18470511740779	19.442674588567172
50-51	19.577154760704808	22.263884689043444	38.25366426515504	19.905296285096707
52-53	20.233760245225387	23.395139502185184	36.73173064834192	19.639369604247513
54-55	20.749755014378707	24.377637829125227	34.81027945632065	20.062327700175413
56-57	21.079472814380342	25.04041985386313	33.588353904655285	20.29175342710124
58-59	21.074846416644668	25.903841570151137	32.56994225727166	20.451369755932532
60-61	21.38104143162734	26.28129506588789	32.14929890211409	20.188364600370683
62-63	21.388476681701746	25.92679986258669	32.234864001953824	20.449859453757743
64-65	20.686649858175148	25.77241237561494	32.440845659503495	21.100092106706413
66-67	20.729669575415905	25.290343101184437	33.44185077339821	20.538136550001447
68-69	20.71633331652212	25.093281412100986	33.50106952005308	20.689315751323818
70-71	20.52108030331165	25.08876021727656	34.13323573393759	20.256923745474207
72-73	20.223919526489524	24.83777248334798	33.852856909826734	21.08545108033576
74-75	20.740354925491584	25.133767104245656	32.96112816916181	21.164749801100943
76-77	21.169199572916902	25.693878667850363	32.58510486822697	20.551816891005764
78-79	21.047731759873678	25.687973085441573	32.27507720554447	20.989217949140283
80-81	20.86491433709803	26.049098115421927	32.211191580389844	20.874795967090197
82-83	21.744671919454465	26.382105137850765	31.447979832862273	20.425243109832504
84-85	21.324166052891165	26.899078007208963	30.889612996257114	20.887142943642754
86-87	21.168431260054106	27.06314155503695	30.83944914801914	20.928978036889802
88-89	20.952503726754475	26.52021020266394	30.931120299621707	21.596165770959878
90-91	20.99949191801047	26.35464661132253	31.117577246692868	21.528284223974126
92-93	21.04979306963531	27.003664341804416	30.964864297582366	20.9816782909779
94-95	21.23823010251732	26.497774997998597	30.40778809348476	21.856206805999324
96-97	21.099162302919925	26.36011122803035	30.964596076662808	21.57613039238692
98-99	21.501831112154708	26.734378446592956	30.450440901148397	21.31334954010394
100-101	21.74257227797573	26.51069996305883	30.14090508746855	21.605822671496895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7329.0
1	4430.5
2	1119.5
3	628.5
4	552.5
5	543.5
6	622.5
7	774.0
8	766.0
9	827.0
10	1389.5
11	8338.5
12	161404.5
13	181613.5
14	49594.5
15	44998.0
16	47766.5
17	51129.5
18	53896.5
19	55442.5
20	55981.0
21	56622.0
22	57666.0
23	59130.5
24	61516.5
25	65107.0
26	70373.5
27	76414.5
28	82760.0
29	89865.5
30	97427.0
31	105117.5
32	113490.0
33	123404.0
34	140865.5
35	152713.5
36	157866.0
37	168371.5
38	176011.0
39	182957.0
40	193876.5
41	201824.0
42	204572.0
43	213309.5
44	227308.0
45	234275.5
46	233310.5
47	235057.5
48	240096.5
49	244278.0
50	248813.5
51	249206.5
52	253157.0
53	259828.0
54	246979.5
55	226846.0
56	219841.5
57	215203.0
58	224431.0
59	194806.0
60	134551.0
61	102842.5
62	75424.5
63	54378.5
64	39386.0
65	26203.0
66	15880.0
67	9465.5
68	5720.5
69	3483.5
70	2414.5
71	1939.0
72	1592.0
73	1370.0
74	1207.5
75	1062.0
76	943.5
77	833.0
78	738.5
79	625.0
80	527.5
81	451.0
82	378.0
83	334.5
84	293.0
85	213.0
86	155.0
87	126.0
88	99.5
89	72.0
90	57.5
91	48.5
92	36.5
93	27.5
94	21.0
95	13.0
96	6.0
97	2.5
98	3.5
99	3.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05183593937985274
2	0.09336868587062364
3	0.08443498570096013
4	0.05538126165348711
5	0.08604765937055556
6	0.0011263117692412446
7	9.343268085751235E-4
8	0.0046972320376311005
9	0.008920901172285767
10-11	0.009522454049039614
12-13	0.01645311112908659
14-15	0.011269517191101319
16-17	0.0014398872049959095
18-19	5.567563859317517E-4
20-21	3.839699213322425E-5
22-23	3.07175937065794E-4
24-25	7.167438531535193E-4
26-27	0.0013438947246628488
28-29	0.0014334877063070387
30-31	0.004646036048120135
32-33	1.0239197902193134E-4
34-35	0.0023102190266823257
36-37	2.687789449325698E-4
38-39	0.0024318095017708693
40-41	0.0031677518509910007
42-43	0.001164708761374469
44-45	5.951533780649759E-4
46-47	9.919222967749598E-4
48-49	0.0022014275489715238
50-51	0.00415327464907709
52-53	0.13222644190944657
54-55	0.14236964733130666
56-57	0.12819475773545805
58-59	0.12200004300463119
60-61	0.10964901053511072
62-63	0.11415425761207569
64-65	0.11672685608500172
66-67	0.12489261641200075
68-69	0.13797959123074136
70-71	0.12523818934119976
72-73	0.11650927312958012
74-75	0.12198084450856458
76-77	0.13330155768917687
78-79	0.14763643475224725
80-81	0.1376596162962978
82-83	0.1168420470614014
84-85	0.12331194023584968
86-87	0.12426546554049143
88-89	0.12037457033765803
90-91	0.13102973565462775
92-93	0.14814199514866802
94-95	0.15784363516099603
96-97	0.15845798703512762
98-99	0.14108334809484366
100-101	0.13009540884605264
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7813112.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.83402846356596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.38565668594623	40.05801476333435
2	4.8141707675262335	4.220489969044242
3	1.3156903709537582	1.7301602750888008
4	0.6094519019257694	1.0685892806475434
5	0.3267330177610964	0.7161012200263353
6	0.2218466066664423	0.5834658282697411
7	0.15655586168022886	0.4803731867920474
8	0.1246648088276654	0.43716486228455154
9	0.10255405038205935	0.404582144715105
>10	0.8994716134478747	8.041092782419325
>50	0.02085722096738609	0.6011157359199997
>100	0.014323858722729815	1.4296761494151
>500	0.003389125210652838	1.0177201795063622
>1k	0.003804120134406176	3.4109755277508693
>5k	6.916582062556684E-4	2.285716815215773
>10k+	1.3833164125113368E-4	33.51476127956982
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1082252	13.851740510055405	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	23183	0.2967191562081793	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG	9717	0.12436785751951335	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCACTCGGCCAGCTTCT	8739	0.11185043808408224	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA	8458	0.1082539198209369	No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT	8426	0.10784435190484917	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC	8392	0.10740918599400598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2798997377741418E-5	0.0	0.0	0.0	0.0
2	3.839699213322425E-5	0.0	0.0	0.0	0.0
3	8.959298164418992E-5	0.0	0.0	0.0	0.0
4	1.2798997377741418E-4	0.0	0.0	0.0	0.0
5	1.53587968532897E-4	0.0	0.0	0.0	0.0
6	1.53587968532897E-4	0.0	0.0	1.2798997377741418E-5	0.0
7	2.047839580438627E-4	0.0	0.0	1.2798997377741418E-5	0.0
8	2.047839580438627E-4	0.0	0.0	2.5597994755482836E-5	0.0
9	2.5597994755482837E-4	0.0	0.0	2.5597994755482836E-5	0.0
10-11	2.943769396880526E-4	0.0	0.0	2.5597994755482836E-5	0.0
12-13	3.263744331324062E-4	0.0	0.0	3.1997493444353546E-5	0.0
14-15	3.5197242788788895E-4	6.399498688870709E-6	0.0	3.839699213322425E-5	0.0
16-17	3.5837192657675967E-4	1.2798997377741418E-5	0.0	3.839699213322425E-5	0.0
18-19	4.415654095320789E-4	1.2798997377741418E-5	0.0	3.839699213322425E-5	0.0
20-21	4.8636190035417385E-4	1.2798997377741418E-5	0.0	5.119598951096567E-5	0.0
22-23	5.05560396420786E-4	1.2798997377741418E-5	0.0	5.119598951096567E-5	0.0
24-25	5.503568872428809E-4	1.2798997377741418E-5	0.0	5.7595488199836385E-5	0.0
26-27	5.759548819983638E-4	1.2798997377741418E-5	0.0	6.399498688870709E-5	0.0
28-29	6.079523754427173E-4	1.2798997377741418E-5	0.0	6.399498688870709E-5	0.0
30-31	6.59148364953683E-4	1.2798997377741418E-5	0.0	7.67939842664485E-5	0.0
32-33	7.487413465978728E-4	1.2798997377741418E-5	0.0	7.67939842664485E-5	0.0
34-35	8.255353308643215E-4	1.2798997377741418E-5	0.0	8.319348295531921E-5	0.0
36-37	8.959298164418993E-4	1.2798997377741418E-5	0.0	8.959298164418992E-5	1.2798997377741418E-5
38-39	9.599248033306063E-4	1.2798997377741418E-5	0.0	8.959298164418992E-5	1.2798997377741418E-5
40-41	0.0010239197902193135	1.2798997377741418E-5	0.0	8.959298164418992E-5	1.2798997377741418E-5
42-43	0.0010943142757968913	1.2798997377741418E-5	0.0	1.1519097639967276E-4	1.2798997377741418E-5
44-45	0.0011903067561299518	1.2798997377741418E-5	0.0	1.1519097639967276E-4	1.2798997377741418E-5
46-47	0.001228703748263176	1.2798997377741418E-5	0.0	1.2798997377741418E-4	1.2798997377741418E-5
48-49	0.0012671007403964002	1.2798997377741418E-5	0.0	1.343894724662849E-4	1.2798997377741418E-5
50-51	0.0013310957272851074	1.2798997377741418E-5	0.0	1.407889711551556E-4	1.2798997377741418E-5
52-53	0.0013950907141738146	1.2798997377741418E-5	0.0	1.407889711551556E-4	1.2798997377741418E-5
54-55	0.0015038821918846165	1.2798997377741418E-5	0.0	1.407889711551556E-4	1.2798997377741418E-5
56-57	0.0015614776800844529	1.2798997377741418E-5	0.0	1.407889711551556E-4	2.5597994755482836E-5
58-59	0.001753462640750574	1.2798997377741418E-5	0.0	1.407889711551556E-4	3.1997493444353546E-5
60-61	0.0018686536171502468	1.2798997377741418E-5	0.0	1.53587968532897E-4	5.119598951096567E-5
62-63	0.0019582465987944366	1.2798997377741418E-5	0.0	1.7918596328837983E-4	5.119598951096567E-5
64-65	0.0020926360712607214	1.9198496066612126E-5	0.0	1.7918596328837983E-4	7.67939842664485E-5
66-67	0.0022654225358602308	2.5597994755482836E-5	0.0	1.7918596328837983E-4	7.67939842664485E-5
68-69	0.002457407496526352	3.1997493444353546E-5	0.0	1.7918596328837983E-4	7.67939842664485E-5
70-71	0.0026109954650592494	3.839699213322425E-5	0.0	1.7918596328837983E-4	7.67939842664485E-5
72-73	0.0028029804257253704	3.839699213322425E-5	0.0	1.7918596328837983E-4	8.959298164418992E-5
74-75	0.0029309703995027848	3.839699213322425E-5	0.0	1.7918596328837983E-4	1.0239197902193134E-4
76-77	0.0031677518509910007	3.839699213322425E-5	0.0	1.7918596328837983E-4	1.0879147771080205E-4
78-79	0.0034557292919901827	3.839699213322425E-5	0.0	1.7918596328837983E-4	1.2159047508854346E-4
80-81	0.003948490691033227	3.839699213322425E-5	0.0	1.7918596328837983E-4	1.2798997377741418E-4
82-83	0.004582041061231427	3.839699213322425E-5	0.0	1.7918596328837983E-4	1.2798997377741418E-4
84-85	0.0053051844130738175	3.839699213322425E-5	0.0	1.8558546197725055E-4	1.2798997377741418E-4
86-87	0.006348302699359743	3.839699213322425E-5	0.0	1.9198496066612125E-4	1.2798997377741418E-4
88-89	0.007545008954178565	3.839699213322425E-5	0.0	1.9198496066612125E-4	1.343894724662849E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGTA	239120	0.0	92.05016	5
GGTATCA	240490	0.0	91.700226	8
AAGCAGT	240840	0.0	91.69594	1
GTATCAA	240895	0.0	91.56576	9
TGGTATC	240890	0.0	91.48687	7
GTGGTAT	242410	0.0	90.98646	6
GCAGTGG	242570	0.0	90.83416	3
CAGTGGT	242735	0.0	90.67695	4
AGCAGTG	243740	0.0	90.57882	2
TCAACGC	239750	0.0	46.44741	12-13
AACGCAG	240445	0.0	46.31853	14-15
CAACGCA	239680	0.0	46.3164	12-13
ATCAACG	239710	0.0	46.299316	10-11
CGCAGAG	241105	0.0	46.179035	16-17
ACGCAGA	240680	0.0	46.145115	14-15
TATCAAC	241715	0.0	46.043896	10-11
CAGAGTA	239995	0.0	45.93241	18-19
AGAGTAC	236350	0.0	45.823547	18-19
GAGTACT	133905	0.0	45.709858	20-21
GCAGAGT	242890	0.0	45.611057	16-17
>>END_MODULE