FastQCFastQC Report
Sat 18 Jun 2016
SRR578563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578563_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7813112
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA917731.174602386347463No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156990.2009314598331625No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT87000.11135127718635034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4000.021.3409633
CAACGCA60350.020.50313212-13
ATCAACG60600.020.41854710-11
TCAACGC59400.018.07751310-11
GGTATCA74750.017.254668
AACGCAG62850.017.12290812-13
AGTGGTA77750.016.894155
CGCGTAG2401.8189894E-1216.79053754-55
GTATCAA79700.016.3007499
ACGCAGA79400.015.70334514-15
TCGCGTA2657.2759576E-1215.20652552-53
TGGTATC87050.015.1979197
AAGCAGT92400.014.9875911
AGAGTAC83400.014.72360918-19
CTCGCGT2751.2732926E-1114.65356252-53
ACTCGCG2801.6370905E-1114.39189150-51
TATCAAC85200.014.3023989
CGTAGCG3055.456968E-1213.98941756-57
GTGGTAT104650.013.1403666
ACGTACG3453.6379788E-1213.05620820-21
Produced by FastQC (version 0.11.5)