##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578563_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7813112
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.986106816336434	31.0	31.0	34.0	30.0	34.0
2	32.25800679677957	34.0	31.0	34.0	30.0	34.0
3	32.32462852190011	34.0	31.0	34.0	30.0	34.0
4	35.90027571600151	37.0	35.0	37.0	35.0	37.0
5	35.78193516232712	37.0	35.0	37.0	33.0	37.0
6	35.75436727388523	37.0	35.0	37.0	33.0	37.0
7	35.748643562257904	37.0	35.0	37.0	33.0	37.0
8	35.66726318527112	37.0	35.0	37.0	33.0	37.0
9	37.30219379934653	39.0	37.0	39.0	33.0	39.0
10-11	37.29615369650403	39.0	37.0	39.0	33.5	39.0
12-13	37.28975144603072	39.0	37.0	39.0	33.0	39.0
14-15	38.6011689708275	40.0	38.0	41.0	34.0	41.0
16-17	38.55435004643476	40.0	38.0	41.0	34.0	41.0
18-19	38.49571815942227	40.0	38.0	41.0	33.5	41.0
20-21	38.43023253730396	40.0	38.0	41.0	34.0	41.0
22-23	38.30938753981768	40.0	38.0	41.0	33.0	41.0
24-25	38.226309055853804	40.0	38.0	41.0	33.0	41.0
26-27	38.05752208594988	40.0	37.0	41.0	33.0	41.0
28-29	37.962984275663786	40.0	37.0	41.0	33.0	41.0
30-31	37.866030142662744	40.0	37.0	41.0	33.0	41.0
32-33	37.732913530485675	40.0	37.0	41.0	32.0	41.0
34-35	37.60426844002748	40.0	37.0	41.0	32.0	41.0
36-37	37.47713401522978	40.0	37.0	41.0	31.5	41.0
38-39	37.34902417884193	40.0	36.0	41.0	31.0	41.0
40-41	37.201345699383296	39.5	36.0	41.0	31.0	41.0
42-43	37.04210051001445	39.0	36.0	41.0	31.0	41.0
44-45	36.88796736818824	39.0	35.5	41.0	30.0	41.0
46-47	36.97428617431825	39.0	36.0	41.0	31.0	41.0
48-49	36.95349797878234	39.0	36.0	41.0	31.0	41.0
50-51	36.92932374449515	39.0	35.5	41.0	31.0	41.0
52-53	36.94308682635037	39.0	36.0	41.0	31.0	41.0
54-55	36.814344975472	39.0	35.0	41.0	31.0	41.0
56-57	36.660707922784155	39.0	35.0	41.0	30.0	41.0
58-59	36.43880165803331	39.0	35.0	41.0	30.0	41.0
60-61	36.20762968712083	38.5	35.0	40.5	30.0	41.0
62-63	35.93085796031082	38.0	35.0	40.0	29.0	41.0
64-65	35.61434182691865	37.5	34.0	40.0	29.0	41.0
66-67	35.29075437546524	37.0	34.0	40.0	29.0	41.0
68-69	34.954258239226576	36.5	34.0	39.0	28.0	41.0
70-71	34.57941246714498	36.0	34.0	39.0	28.0	41.0
72-73	34.15443577411919	35.5	33.5	39.0	28.0	40.0
74-75	33.76826820094221	35.0	33.0	37.5	27.0	40.0
76-77	32.33225090079344	34.5	31.5	36.0	25.5	39.0
78-79	32.934814002410306	35.0	32.5	36.5	26.0	39.0
80-81	32.882143440411454	35.0	33.0	36.0	26.0	39.0
82-83	32.60983587589682	35.0	33.0	36.0	26.0	37.0
84-85	32.32606194561143	35.0	33.0	35.5	26.0	37.0
86-87	32.05000069114586	35.0	32.0	35.0	26.0	36.5
88-89	31.80417917981977	35.0	32.0	35.0	25.0	36.0
90-91	31.566112964974778	35.0	32.0	35.0	25.0	36.0
92-93	31.328473020737448	35.0	32.0	35.0	24.5	36.0
94-95	31.077775091410437	35.0	32.0	35.0	23.5	35.5
96-97	30.474067311463088	34.0	31.5	35.0	18.0	35.0
98-99	30.443895530999683	34.0	31.0	35.0	19.0	35.0
100-101	29.864918869715424	34.0	30.5	35.0	9.5	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.42752317836743003
1101	2	0.406803880316005
1101	3	0.377377628004119
1101	4	0.2838515913256501
1101	5	0.24959421569633378
1101	6	0.28743105274459424
1101	7	0.31668401965934123
1101	8	0.39394777291792593
1101	9	0.4685407877944314
1101	10-11	0.4063751538031539
1101	12-13	0.42206844576012514
1101	14-15	0.577107160061729
1101	16-17	0.5466778146531368
1101	18-19	0.5204613562757032
1101	20-21	0.5955186276217788
1101	22-23	0.6469857760923006
1101	24-25	0.6523330619155345
1101	26-27	0.7146263639303072
1101	28-29	0.7111511927614629
1101	30-31	0.7070022635239468
1101	32-33	0.6563807548725933
1101	34-35	0.6745889086328347
1101	36-37	0.7074069879561122
1101	38-39	0.6776073338256126
1101	40-41	0.7443652286823763
1101	42-43	0.7311744558593958
1101	44-45	0.7494278728946213
1101	46-47	0.738277167349878
1101	48-49	0.6944495587401391
1101	50-51	0.6921312997767544
1101	52-53	0.6308690282831932
1101	54-55	0.7365844568385072
1101	56-57	0.7336401284744554
1101	58-59	0.7914877757436543
1101	60-61	0.6879440196426216
1101	62-63	0.7073780028337566
1101	64-65	0.7119073501335151
1101	66-67	0.6808000845637778
1101	68-69	0.7347764835072539
1101	70-71	0.6953563184025242
1101	72-73	0.7004015047430201
1101	74-75	0.7433100827569135
1101	76-77	0.7266185415791355
1101	78-79	0.6261879973489783
1101	80-81	0.5633624392369256
1101	82-83	0.5579727412339892
1101	84-85	0.5072379428894891
1101	86-87	0.45990746852286435
1101	88-89	0.48612930888996786
1101	90-91	0.48469822542659813
1101	92-93	0.5524274388299482
1101	94-95	0.5430343305014169
1101	96-97	0.9351539989204873
1101	98-99	0.6849814276321986
1101	100-101	0.6292761824910471
1102	1	0.37539140946766025
1102	2	0.34936170566705016
1102	3	0.323862011742456
1102	4	0.22096801975285985
1102	5	0.26228685911713967
1102	6	0.2498038829903919
1102	7	0.22548923196689685
1102	8	0.25236276578117867
1102	9	0.3796580610880653
1102	10-11	0.3509348894865951
1102	12-13	0.37516150367685697
1102	14-15	0.4284532956332754
1102	16-17	0.47889657475277403
1102	18-19	0.48943642768448825
1102	20-21	0.49787719622491977
1102	22-23	0.50819763916666
1102	24-25	0.5700206183853496
1102	26-27	0.6036733294855381
1102	28-29	0.6370847337816059
1102	30-31	0.6698533091539858
1102	32-33	0.6540372470159994
1102	34-35	0.68851461349999
1102	36-37	0.7081951953717436
1102	38-39	0.73113415499013
1102	40-41	0.7198640276229753
1102	42-43	0.6978973858550503
1102	44-45	0.7410427677768752
1102	46-47	0.7940426029704852
1102	48-49	0.7533189198876471
1102	50-51	0.7599209863144623
1102	52-53	0.7127549210333441
1102	54-55	0.7303349255729117
1102	56-57	0.6878155919041475
1102	58-59	0.7074794791982484
1102	60-61	0.769817088220627
1102	62-63	0.7383953483979084
1102	64-65	0.736833733855633
1102	66-67	0.7461283429881931
1102	68-69	0.7789514429145967
1102	70-71	0.7243531111081367
1102	72-73	0.7214865675319615
1102	74-75	0.6062979581840082
1102	76-77	0.5954619679369131
1102	78-79	0.598696111661603
1102	80-81	0.6052152034558418
1102	82-83	0.6198668114923223
1102	84-85	0.6034653930940834
1102	86-87	0.5744387818664478
1102	88-89	0.5055003321766414
1102	90-91	0.49461322498062543
1102	92-93	0.5576168091567517
1102	94-95	0.5723880145644209
1102	96-97	0.990943062654182
1102	98-99	0.7978212280428743
1102	100-101	0.791076966976128
1103	1	0.32585129651288725
1103	2	0.29746510570439
1103	3	0.2697274087395982
1103	4	0.21988203744679424
1103	5	0.22794619474277056
1103	6	0.2688518447516799
1103	7	0.2532888918804943
1103	8	0.043683263337143785
1103	9	0.1547279323698021
1103	10-11	0.3720781033925533
1103	12-13	0.34795748285783645
1103	14-15	0.4505319197125246
1103	16-17	0.4758781183373273
1103	18-19	0.37938337451294046
1103	20-21	0.4152472543896977
1103	22-23	0.4531428274418019
1103	24-25	0.2920475809662193
1103	26-27	0.33656139558566167
1103	28-29	0.5323106237259196
1103	30-31	0.3407285301023393
1103	32-33	0.5529152989496922
1103	34-35	0.5237887833388228
1103	36-37	0.6141186352809953
1103	38-39	0.7063810470123997
1103	40-41	0.5315859679170529
1103	42-43	0.6665087836667354
1103	44-45	0.7286220656628544
1103	46-47	0.5889140381076459
1103	48-49	0.6052914567987315
1103	50-51	0.6002180650706421
1103	52-53	0.6680414467651943
1103	54-55	0.7718479627721706
1103	56-57	0.6700780634219328
1103	58-59	0.4071233029093051
1103	60-61	0.4044424405171938
1103	62-63	0.280285196231695
1103	64-65	0.5313534583299173
1103	66-67	0.4997299215358666
1103	68-69	0.4365495394670589
1103	70-71	0.4360500409135213
1103	72-73	0.6227537784084518
1103	74-75	0.6000878979466293
1103	76-77	0.4288759786852623
1103	78-79	0.49955241676340023
1103	80-81	0.5408470324441836
1103	82-83	0.5813725311156048
1103	84-85	0.592440056376077
1103	86-87	0.4435885709806655
1103	88-89	0.4275674299978043
1103	90-91	0.47065194110631126
1103	92-93	0.43850863388228944
1103	94-95	0.47824324288787423
1103	96-97	0.6078851898449251
1103	98-99	0.3441898090569566
1103	100-101	0.6228693120408479
1104	1	0.33563318698274713
1104	2	0.3122847263586124
1104	3	0.2646989977100134
1104	4	0.19340200613179093
1104	5	0.2493182056527914
1104	6	0.2630306363712478
1104	7	0.23789649845882366
1104	8	0.30224239477870185
1104	9	0.30056359204947114
1104	10-11	0.3312455740566378
1104	12-13	0.3669924306796517
1104	14-15	0.4121013405584506
1104	16-17	0.4428554537893916
1104	18-19	0.4223345575085915
1104	20-21	0.3765880543928688
1104	22-23	0.4877784741010842
1104	24-25	0.4247839995786862
1104	26-27	0.5112217710517584
1104	28-29	0.5049439384484771
1104	30-31	0.535063847170882
1104	32-33	0.5814583374997611
1104	34-35	0.5654882790965488
1104	36-37	0.5803463323046927
1104	38-39	0.5828832237124999
1104	40-41	0.5834534257425048
1104	42-43	0.64239903558299
1104	44-45	0.5924349986668886
1104	46-47	0.4323412291541189
1104	48-49	0.557802836825104
1104	50-51	0.4619832955685297
1104	52-53	0.5161722787756986
1104	54-55	0.6336574509936028
1104	56-57	0.6183759751546631
1104	58-59	0.6154016204581794
1104	60-61	0.5575516096236655
1104	62-63	0.39021324376467703
1104	64-65	0.5936723523709588
1104	66-67	0.6393431534798779
1104	68-69	0.689542351325386
1104	70-71	0.6396821277878715
1104	72-73	0.7267944499097325
1104	74-75	0.6655365739409334
1104	76-77	0.587331017315968
1104	78-79	0.577282718441289
1104	80-81	0.662567995304542
1104	82-83	0.7261139253966533
1104	84-85	0.7535969848289312
1104	86-87	0.7110785260388752
1104	88-89	0.6764759446484554
1104	90-91	0.7301178477881933
1104	92-93	0.7166154690692359
1104	94-95	0.7126782716078921
1104	96-97	1.1434551933032573
1104	98-99	0.8841948833697799
1104	100-101	0.8946472555842817
1105	1	0.16949402722491413
1105	2	0.12661139061727766
1105	3	0.10590207636384719
1105	4	0.060984785362755645
1105	5	0.11532903303903907
1105	6	0.06950062071544494
1105	7	0.0831253760208952
1105	8	0.16986656748412088
1105	9	0.14526212753322199
1105	10-11	0.10290733209986769
1105	12-13	0.1379685620493163
1105	14-15	0.12299747415592321
1105	16-17	0.12984623766146086
1105	18-19	0.06896529801012719
1105	20-21	0.10925408412295923
1105	22-23	0.12260232061080956
1105	24-25	0.16713167631795045
1105	26-27	0.09441646065960896
1105	28-29	0.11850622290077695
1105	30-31	0.06060131340007047
1105	32-33	0.12250640562473336
1105	34-35	0.06435405773142833
1105	36-37	0.11449024295603039
1105	38-39	0.13853266275530984
1105	40-41	0.16936297126672173
1105	42-43	0.045585699928601287
1105	44-45	0.143919408353419
1105	46-47	0.1716731479268887
1105	48-49	0.12860411462968813
1105	50-51	0.039251134250157804
1105	52-53	0.140825624178035
1105	54-55	0.20574825444723643
1105	56-57	0.22265181159739456
1105	58-59	0.20657850923392118
1105	60-61	0.23444581155715838
1105	62-63	0.2115671433544435
1105	64-65	0.13704451524048267
1105	66-67	0.21040553858070155
1105	68-69	0.19377111292902782
1105	70-71	0.21357576826330416
1105	72-73	0.2448720733219858
1105	74-75	0.25371158526743187
1105	76-77	0.2762062438381392
1105	78-79	0.27722691128019505
1105	80-81	0.2217034918028844
1105	82-83	0.2041600937060437
1105	84-85	0.2996632478115657
1105	86-87	0.1987953511379672
1105	88-89	0.1780671874588542
1105	90-91	0.22392228186096474
1105	92-93	0.23776489229811304
1105	94-95	0.2970441894180915
1105	96-97	0.6547253739608898
1105	98-99	0.4188065064179938
1105	100-101	0.3872292275794891
1106	1	-0.045017085512196076
1106	2	-0.10778497591053338
1106	3	-0.07126157699212854
1106	4	-0.07303334001224471
1106	5	-0.0720177746641042
1106	6	-0.0990841218839762
1106	7	-0.08044166637899508
1106	8	-0.07565616311515555
1106	9	-0.03147688690281569
1106	10-11	-0.0910680569860034
1106	12-13	-0.10944404004417407
1106	14-15	-0.16540634138529242
1106	16-17	-0.14802087045769952
1106	18-19	-0.15099269675978633
1106	20-21	-0.19838287371683805
1106	22-23	-0.16834321497142213
1106	24-25	-0.11383702638121207
1106	26-27	-0.10893306932350555
1106	28-29	-0.12677595004349484
1106	30-31	-0.2557604738571513
1106	32-33	-0.3198931717212261
1106	34-35	-0.3736099483664006
1106	36-37	-0.3209947395689099
1106	38-39	-0.3215032343971913
1106	40-41	-0.3415203478291886
1106	42-43	-0.29935298790378084
1106	44-45	-0.2765606831754326
1106	46-47	-0.3060447522276277
1106	48-49	-0.3107518227054058
1106	50-51	-0.34166473918155305
1106	52-53	-0.24543592221275645
1106	54-55	-0.2887341489963333
1106	56-57	-0.27151772245241546
1106	58-59	-0.21371982489805674
1106	60-61	-0.2997538263913029
1106	62-63	-0.23627613580732287
1106	64-65	-0.14820482173223581
1106	66-67	-0.24336981357627252
1106	68-69	-0.22524956280234676
1106	70-71	-0.2234504895555176
1106	72-73	-0.19655034059732657
1106	74-75	-0.16744348866610892
1106	76-77	-0.18281463109207863
1106	78-79	-0.1106243918355787
1106	80-81	-0.17264806294444668
1106	82-83	-0.19511887861098387
1106	84-85	-0.20645369706119965
1106	86-87	-0.2673776826003049
1106	88-89	-0.2922677749557394
1106	90-91	-0.22301449865135936
1106	92-93	-0.24454724350663426
1106	94-95	-0.19000151107491092
1106	96-97	0.0549542859810046
1106	98-99	-0.13601755658632086
1106	100-101	-0.17117138031312962
1107	1	-0.18553412729214713
1107	2	-0.2503139108538406
1107	3	-0.21322707319061607
1107	4	-0.14140042671650832
1107	5	-0.13647852484695022
1107	6	-0.13126867040490708
1107	7	-0.16447162996099962
1107	8	-0.1525534049212709
1107	9	-0.21147212228127898
1107	10-11	-0.21743352689978934
1107	12-13	-0.17782928044388058
1107	14-15	-0.23170378019386817
1107	16-17	-0.2888591257637856
1107	18-19	-0.2691435142478298
1107	20-21	-0.25909788674174195
1107	22-23	-0.19504503997459466
1107	24-25	-0.16366590132754055
1107	26-27	-0.19453941661314644
1107	28-29	-0.17099556495290358
1107	30-31	-0.21462946028479735
1107	32-33	-0.2952548003229296
1107	34-35	-0.23989403401380827
1107	36-37	-0.25270139074188336
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2308	16-17	-0.9235047460232622
2308	18-19	-0.947209748440514
2308	20-21	-0.944261371093404
2308	22-23	-1.0470294762795547
2308	24-25	-1.2051315462570855
2308	26-27	-1.174630108673881
2308	28-29	-1.241114959601127
2308	30-31	-1.0712057336253267
2308	32-33	-1.1481210976371798
2308	34-35	-1.2308330408839154
2308	36-37	-1.2453192838941476
2308	38-39	-1.2854185658190787
2308	40-41	-1.2121479429655437
2308	42-43	-1.1042567322490484
2308	44-45	-1.1439797548529569
2308	46-47	-1.1198688180153127
2308	48-49	-1.2439589190430524
2308	50-51	-1.2409407289406076
2308	52-53	-1.2210948379272324
2308	54-55	-1.218526883630389
2308	56-57	-1.1264502911805039
2308	58-59	-1.0429340172529677
2308	60-61	-1.047552438423999
2308	62-63	-1.0777207485899183
2308	64-65	-1.0752526045643762
2308	66-67	-1.0575439040872752
2308	68-69	-1.1370529812471446
2308	70-71	-1.059300332868851
2308	72-73	-1.0533897073438823
2308	74-75	-1.2307796959108472
2308	76-77	-1.148138807857194
2308	78-79	-1.5463912378603126
2308	80-81	-1.2254674184149295
2308	82-83	-1.093454307076783
2308	84-85	-1.0223859580535049
2308	86-87	-1.0039765234431428
2308	88-89	-1.1382005915298699
2308	90-91	-1.2007693243306576
2308	92-93	-1.0898871566897448
2308	94-95	-1.2115386659078489
2308	96-97	-1.0552639524120444
2308	98-99	-1.3483503322183665
2308	100-101	-1.3958529288685853
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45.0
3	84.0
4	157.0
5	399.0
6	831.0
7	1654.0
8	3249.0
9	4829.0
10	6608.0
11	8188.0
12	9636.0
13	11285.0
14	12759.0
15	13940.0
16	16279.0
17	19206.0
18	22140.0
19	25380.0
20	27186.0
21	28212.0
22	30711.0
23	36125.0
24	44013.0
25	55461.0
26	70687.0
27	90786.0
28	115800.0
29	145375.0
30	179816.0
31	222171.0
32	274161.0
33	343150.0
34	451907.0
35	667028.0
36	927756.0
37	1584835.0
38	1851713.0
39	507814.0
40	1736.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	18.32431258242745	31.06382820759346	27.792574485997683	22.819284723981408
2	23.207473767042348	28.615900649837684	27.917046388320614	20.25957919479935
3	21.248552027801065	27.77736107466737	28.438634778212258	22.53545211931931
4	21.79410631235784	28.390424200676023	28.06703023689405	21.748439250072092
5	22.644997073481157	28.1445614554238	27.972080511073994	21.23836096002105
6	22.318226079441843	28.035615002063196	27.79938390746223	21.846775011032733
7	22.58360049107193	28.291044592730785	27.73224036721859	21.39311454897869
8	21.70489589921353	28.154706887457078	28.105490742564747	22.03490647076465
9	21.521648999443315	27.91038133323332	28.592421106150418	21.97554856117295
10-11	21.66513164024783	28.452798321590677	27.943839791366106	21.93823024679539
12-13	21.65245423334518	28.544983100203865	27.73923501928553	22.06332764716543
14-15	21.82589984630964	28.531518554962474	27.544005512784153	22.09857608594373
16-17	22.065074710307496	28.348224113515847	27.47082724527691	22.11587393089975
18-19	22.013321964410597	28.351398264865523	27.522791174630544	22.112488596093336
20-21	22.099292829796884	28.29038544436583	27.51629568346134	22.094026042375944
22-23	22.050625199584545	28.29875574435516	27.54768430732724	22.10293474873306
24-25	22.004374016068777	28.22757759981036	27.491611925334407	22.276436458786453
26-27	22.177916821572012	28.126806984618945	27.63323038594295	22.062045807866085
28-29	22.207873124050952	28.204888141882517	27.534297473273135	22.052941260793393
30-31	22.16646196803527	28.207767916292507	27.486921984479423	22.138848131192795
32-33	22.179324960399903	28.248078358533707	27.408995288945043	22.163601392121347
34-35	22.18249271225089	28.28057501287579	27.401981438382045	22.134950836491274
36-37	22.18544928064515	28.27576898936045	27.377548152387938	22.161233577606467
38-39	22.185103707715953	28.28036382941906	27.381259861627477	22.153272601237507
40-41	22.191823181339267	28.334094020410816	27.376057069193426	22.098025729056488
42-43	22.22211840813238	28.30578263821125	27.311863697845368	22.160235255811
44-45	22.204084620827143	28.367768182511654	27.2887871055733	22.139360091087905
46-47	22.2223054157105	28.388184400030642	27.27246372971895	22.11704645453991
48-49	22.22037774448901	28.3945948810154	27.240310903005106	22.14471647149049
50-51	22.250941750227053	28.391887893070006	27.235901648408472	22.121268708294465
52-53	22.24034418039828	28.436114828508796	27.171887463023698	22.151653528069225
54-55	22.26756764782074	28.434681340802488	27.104404749349552	22.193346262027216
56-57	22.26359995863364	28.456119661410206	27.113600828965463	22.166679550990693
58-59	22.253610341180313	28.5850951579857	27.062059266525296	22.099235234308683
60-61	22.25306285022481	28.53187141375841	27.060292855345054	22.15477288067173
62-63	22.234641526343143	28.599786602139186	27.055855063009197	22.109716808508473
64-65	22.22883861017115	28.646010353264135	27.034746745254424	22.090404291310296
66-67	22.202114961312404	28.668691983640805	27.02983502830759	22.099358026739203
68-69	22.173950661511725	28.705394242917237	27.04237082157902	22.078284273992015
70-71	22.23318813068328	28.7665074448329	26.962653188966456	22.03765123551736
72-73	22.174897203219793	28.761768921835795	26.95709051562633	22.10624335931808
74-75	22.23341566078241	28.761943548976472	26.959684038709813	22.044956751531306
76-77	22.21417644686514	28.84617430109722	26.911926760932314	22.02772249110533
78-79	22.219813939568297	28.826663133854748	26.88358669711903	22.069936229457927
80-81	22.163155503679747	28.900438999631966	26.86896738677948	22.067438109908807
82-83	22.190426693857443	28.968672306410507	26.834265472417663	22.006635527314387
84-85	22.1725667420939	28.980262879995944	26.82281059845225	22.024359779457903
86-87	22.137708993403834	29.031923652334928	26.788176235219492	22.042191119041746
88-89	22.127683380272376	29.093435555329698	26.794087865862913	21.98479319853501
90-91	22.11918660562763	29.080861898141958	26.751597826841415	22.048353669389
92-93	22.12397695119584	29.138283403849673	26.74139004087549	21.996349604079
94-95	22.107124627598576	29.206586439831188	26.71036210862894	21.9759268239413
96-97	22.082375348999093	29.24394266610724	26.69035853990847	21.98332344498519
98-99	22.067907461097885	29.266910419304494	26.66637206284796	21.99881005674966
100-101	22.1161330762449	29.309144529889412	26.57459034455712	22.00013204930857
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102047.0
1	58022.0
2	10658.5
3	6868.0
4	5916.5
5	5172.0
6	4782.0
7	4752.0
8	4687.5
9	4313.0
10	4142.5
11	4545.0
12	7589.5
13	7564.5
14	5001.5
15	5373.0
16	5730.5
17	6440.0
18	8230.0
19	9721.5
20	11503.5
21	14659.0
22	18655.0
23	23924.5
24	29696.5
25	36655.5
26	47254.0
27	58865.5
28	67058.0
29	75674.0
30	89742.5
31	106571.5
32	123144.0
33	139704.5
34	156503.5
35	171872.0
36	186965.0
37	203776.0
38	216537.0
39	222190.5
40	231967.5
41	242908.0
42	249222.0
43	260428.0
44	272944.0
45	282193.0
46	286537.0
47	286165.5
48	289995.0
49	294572.5
50	294690.0
51	292177.5
52	289790.0
53	281150.0
54	262554.5
55	244308.0
56	223751.5
57	201940.0
58	186546.0
59	163633.0
60	132355.5
61	103267.5
62	75127.0
63	52621.5
64	36453.5
65	23174.5
66	13731.0
67	8519.5
68	4735.5
69	2200.5
70	991.0
71	433.5
72	217.5
73	111.0
74	72.5
75	51.5
76	25.5
77	17.0
78	15.5
79	11.0
80	6.0
81	6.0
82	4.0
83	0.5
84	1.0
85	1.5
86	1.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012018258537699191
2	0.018801727147902143
3	0.0059131367885165344
4	5.247588924873981E-4
5	9.599248033306063E-4
6	0.0
7	0.0
8	0.0081273633348658
9	0.01037998687334829
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.959298164418992E-5
24-25	0.004908415494363834
26-27	0.006981853069557943
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	6.399498688870709E-6
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	7.039448557757778E-5
62-63	8.319348295531921E-5
64-65	2.6237944624369903E-4
66-67	1.7918596328837983E-4
68-69	2.943769396880526E-4
70-71	1.0879147771080205E-4
72-73	3.1997493444353546E-5
74-75	3.839699213322425E-5
76-77	0.12600612918386425
78-79	0.056475575929284
80-81	9.599248033306062E-5
82-83	0.002156631058149429
84-85	0.00929207209624027
86-87	0.11950423851597161
88-89	0.13588055566079177
90-91	0.14919151293364283
92-93	0.14709247736369324
94-95	0.11346951125236653
96-97	1.2086285208761887
98-99	0.07701156722187011
100-101	0.06940256328080284
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7813112.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.30752370016091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.04671909032288	59.460357648131556
2	4.8269512810429935	6.304724703724748
3	1.3557725354490437	2.656264409725971
4	0.6555151083687619	1.7124027390242589
5	0.38280919598865043	1.2500160319834168
6	0.23396744967672278	0.9167900858897268
7	0.1576787316150779	0.7208325251374109
8	0.12121518835610218	0.6333011029108491
9	0.09665255352792108	0.56809250371852
>10	1.0192656029527902	15.114588140191373
>50	0.09258113376890811	3.898828225419048
>100	0.010451852000532539	0.8988744563416571
>500	0.0	0.0
>1k	1.4009230988365205E-4	0.29684196055631257
>5k	1.4009230988365205E-4	0.6517348454377847
>10k+	1.4009230988365205E-4	4.91635062180737
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91773	1.174602386347463	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15699	0.2009314598331625	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8700	0.11135127718635034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.839699213322425E-5	0.0	0.0	0.0	0.0
2	6.399498688870709E-5	0.0	0.0	0.0	0.0
3	1.53587968532897E-4	0.0	0.0	0.0	1.2798997377741418E-5
4	1.9198496066612125E-4	0.0	0.0	0.0	3.839699213322425E-5
5	2.175829554216041E-4	0.0	0.0	0.0	5.119598951096567E-5
6	2.5597994755482837E-4	0.0	0.0	0.0	5.119598951096567E-5
7	2.6877894493256975E-4	0.0	0.0	0.0	5.119598951096567E-5
8	2.943769396880526E-4	0.0	0.0	0.0	5.119598951096567E-5
9	3.455729291990183E-4	0.0	0.0	0.0	5.119598951096567E-5
10-11	3.5837192657675967E-4	0.0	0.0	0.0	8.959298164418992E-5
12-13	3.647714252656304E-4	0.0	0.0	0.0	1.0239197902193133E-4
14-15	4.2236691346546676E-4	0.0	0.0	0.0	1.407889711551556E-4
16-17	4.671634042875617E-4	0.0	0.0	1.2798997377741418E-5	2.047839580438627E-4
18-19	5.183593937985273E-4	0.0	0.0	1.2798997377741418E-5	2.367814514882162E-4
20-21	5.439573885540102E-4	0.0	0.0	1.2798997377741418E-5	2.6877894493256975E-4
22-23	5.823543806872345E-4	0.0	0.0	1.2798997377741418E-5	2.6877894493256975E-4
24-25	6.463493675759415E-4	0.0	0.0	1.9198496066612126E-5	2.943769396880526E-4
26-27	7.039448557757779E-4	0.0	0.0	2.5597994755482836E-5	2.943769396880526E-4
28-29	7.999373361088386E-4	0.0	0.0	3.839699213322425E-5	3.007764383769233E-4
30-31	8.57532824308675E-4	0.0	0.0	3.839699213322425E-5	3.263744331324062E-4
32-33	9.087288138196406E-4	0.0	0.0	4.479649082209496E-5	3.5837192657675967E-4
34-35	0.0010111207928415718	0.0	0.0	5.119598951096567E-5	3.647714252656304E-4
36-37	0.0011135127718635033	0.0	0.0	5.119598951096567E-5	3.903694200211132E-4
38-39	0.0011967062548188224	0.0	0.0	5.119598951096567E-5	4.0316841739885466E-4
40-41	0.001254301743018659	0.0	0.0	7.03944855775778E-5	4.1596741477659604E-4
42-43	0.0013630932207294609	0.0	0.0	8.959298164418992E-5	4.8636190035417385E-4
44-45	0.0014462867036847801	0.0	0.0	8.959298164418992E-5	5.119598951096567E-4
46-47	0.0014974826931957459	0.0	0.0	9.599248033306064E-5	5.247588924873981E-4
48-49	0.0015614776800844529	0.0	0.0	1.2798997377741418E-4	5.439573885540102E-4
50-51	0.0016318721656620307	0.0	0.0	1.407889711551556E-4	5.631558846206224E-4
52-53	0.0017214651473062208	0.0	0.0	1.53587968532897E-4	5.823543806872345E-4
54-55	0.0018046586302615398	0.0	0.0	1.53587968532897E-4	6.015528767538466E-4
56-57	0.0018494551210836347	0.0	0.0	1.53587968532897E-4	6.14351874131588E-4
58-59	0.0019454476014166954	0.0	0.0	1.53587968532897E-4	6.59148364953683E-4
60-61	0.0020734375751941094	0.0	0.0	1.53587968532897E-4	7.039448557757779E-4
62-63	0.0021694300555271705	0.0	0.0	1.53587968532897E-4	7.359423492201315E-4
64-65	0.0022910205306157136	0.0	0.0	1.7278646459950911E-4	7.871383387310972E-4
66-67	0.0024446084991486105	0.0	0.0	1.7918596328837983E-4	7.935378374199679E-4
68-69	0.002566198974237154	0.0	0.0	1.7918596328837983E-4	8.447338269309335E-4
70-71	0.0026877894493256976	0.0	0.0	1.7918596328837983E-4	8.511333256198042E-4
72-73	0.002822178921791983	0.0	0.0	1.7918596328837983E-4	8.703318216864164E-4
74-75	0.002969367391636009	0.0	0.0	1.7918596328837983E-4	9.023293151307699E-4
76-77	0.0031613523523021303	0.0	0.0	1.9198496066612125E-4	9.151283125085113E-4
78-79	0.0034557292919901827	0.0	0.0	2.047839580438627E-4	9.215278111973821E-4
80-81	0.003967689187099839	0.0	0.0	2.047839580438627E-4	9.343268085751235E-4
82-83	0.0045884405599202985	0.0	0.0	2.047839580438627E-4	9.535253046417357E-4
84-85	0.0053051844130738175	0.0	0.0	2.175829554216041E-4	0.0010047212941527014
86-87	0.006303506208537648	0.0	0.0	2.175829554216041E-4	0.0010367187875970547
88-89	0.0075194109594230825	0.0	0.0	2.175829554216041E-4	0.0010559172836636667
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCACG	400	0.0	21.340963	3
CAACGCA	6035	0.0	20.503132	12-13
ATCAACG	6060	0.0	20.418547	10-11
TCAACGC	5940	0.0	18.077513	10-11
GGTATCA	7475	0.0	17.25466	8
AACGCAG	6285	0.0	17.122908	12-13
AGTGGTA	7775	0.0	16.89415	5
CGCGTAG	240	1.8189894E-12	16.790537	54-55
GTATCAA	7970	0.0	16.300749	9
ACGCAGA	7940	0.0	15.703345	14-15
TCGCGTA	265	7.2759576E-12	15.206525	52-53
TGGTATC	8705	0.0	15.197919	7
AAGCAGT	9240	0.0	14.987591	1
AGAGTAC	8340	0.0	14.723609	18-19
CTCGCGT	275	1.2732926E-11	14.653562	52-53
ACTCGCG	280	1.6370905E-11	14.391891	50-51
TATCAAC	8520	0.0	14.302398	9
CGTAGCG	305	5.456968E-12	13.989417	56-57
GTGGTAT	10465	0.0	13.140366	6
ACGTACG	345	3.6379788E-12	13.056208	20-21
>>END_MODULE