FastQCFastQC Report
Sat 18 Jun 2016
SRR578562_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578562_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357943
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127698212.328528936681733No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT299360.2890149134823391No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG109040.10527186720374886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2803150.091.754165
GGTATCA2815850.091.482758
AAGCAGT2824650.091.4152761
TGGTATC2820150.091.279927
GTATCAA2822000.091.2299969
GCAGTGG2839650.090.795353
GTGGTAT2840450.090.682666
AGCAGTG2856000.090.402092
CAGTGGT2846500.090.2330254
TCAACGC2809850.046.3508712-13
AACGCAG2819300.046.23775514-15
CAACGCA2811750.046.1860612-13
ATCAACG2811450.046.17997410-11
CGCAGAG2834950.046.01031516-17
ACGCAGA2824750.046.00474514-15
TATCAAC2828900.045.93458610-11
CAGAGTA2814000.045.7542418-19
AGAGTAC2765000.045.64175818-19
GAGTACT1601000.045.4406120-21
GCAGAGT2853750.045.36765316-17
Produced by FastQC (version 0.11.5)