##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578562_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10357943 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.574758038347962 31.0 31.0 34.0 27.0 34.0 2 30.874570076317276 31.0 31.0 34.0 27.0 34.0 3 30.956535385452497 31.0 31.0 34.0 27.0 34.0 4 34.34950578507721 37.0 35.0 37.0 30.0 37.0 5 34.44637482558072 37.0 35.0 37.0 30.0 37.0 6 34.42542607156653 37.0 35.0 37.0 30.0 37.0 7 34.407754416103664 37.0 35.0 37.0 30.0 37.0 8 34.366295701762404 37.0 35.0 37.0 30.0 37.0 9 35.75856200405814 39.0 35.0 39.0 30.0 39.0 10-11 35.724921009895496 39.0 35.0 39.0 30.0 39.0 12-13 35.7680766827931 39.0 35.0 39.0 30.0 39.0 14-15 36.82345988001671 40.0 36.0 41.0 30.0 41.0 16-17 36.781385213260975 40.0 36.0 41.0 30.0 41.0 18-19 36.77939529113068 40.0 36.0 41.0 30.0 41.0 20-21 36.664141808851426 39.5 36.0 41.0 30.0 41.0 22-23 36.36416289411903 39.0 36.0 40.5 29.0 41.0 24-25 36.26436769346964 39.0 36.0 40.5 29.0 41.0 26-27 36.15007656442983 39.0 36.0 40.5 28.5 41.0 28-29 35.88162596569609 39.0 35.0 41.0 27.0 41.0 30-31 35.651146130076214 39.0 34.5 40.5 26.5 41.0 32-33 35.236613147996664 38.0 34.0 40.0 25.0 41.0 34-35 35.0180109602843 38.0 34.0 40.0 25.0 41.0 36-37 34.67691852523228 38.0 33.0 40.0 24.0 41.0 38-39 34.00350021234911 37.0 33.0 40.0 24.0 41.0 40-41 34.19468715940994 37.0 33.0 40.0 24.0 41.0 42-43 34.12856515043576 37.0 33.0 40.0 23.5 41.0 44-45 33.98863200927057 37.0 33.0 40.0 23.0 41.0 46-47 33.779197423658346 37.0 33.0 40.0 23.0 41.0 48-49 33.428742125729016 37.0 33.0 40.0 20.5 41.0 50-51 32.211657179422595 36.0 31.5 39.0 15.5 40.5 52-53 31.81075383403828 36.0 31.0 39.0 7.5 40.0 54-55 31.398130159627257 37.0 30.5 40.0 2.0 41.0 56-57 30.899578854604627 36.5 29.5 40.0 2.0 41.0 58-59 30.5486584546758 36.0 28.5 40.0 2.0 41.0 60-61 30.23822176854999 36.0 28.0 39.5 2.0 41.0 62-63 29.89279623376958 35.0 28.0 39.0 2.0 41.0 64-65 29.547997367817143 35.0 28.0 39.0 2.0 41.0 66-67 29.223323878109774 35.0 27.0 38.5 2.0 40.0 68-69 28.887544756714725 35.0 26.0 38.0 2.0 40.0 70-71 28.513013298103687 35.0 26.0 37.0 2.0 40.0 72-73 28.112001967958307 34.0 26.0 37.0 2.0 39.0 74-75 27.747847569734645 34.0 25.5 36.0 2.0 39.0 76-77 27.3522853427558 34.0 25.0 36.0 2.0 38.5 78-79 26.994740992492428 34.0 24.0 35.0 2.0 37.5 80-81 26.632937736768778 34.0 23.5 35.0 2.0 37.0 82-83 26.25892390023772 33.5 21.5 35.0 2.0 36.5 84-85 25.918413289202306 33.0 20.0 35.0 2.0 36.0 86-87 25.586476629577902 33.0 19.5 35.0 2.0 36.0 88-89 25.230293456915142 33.0 18.0 35.0 2.0 35.0 90-91 24.869649311644213 32.5 12.5 35.0 2.0 35.0 92-93 24.51866997144124 32.0 7.0 35.0 2.0 35.0 94-95 24.075978261320806 32.0 2.0 35.0 2.0 35.0 96-97 23.579379708886215 32.0 2.0 35.0 2.0 35.0 98-99 23.034751591121903 31.0 2.0 34.5 2.0 35.0 100-101 21.685993058660394 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6202109647433325 1101 2 0.6697820111278787 1101 3 0.6910944082260642 1101 4 0.7892396607786054 1101 5 0.7054422241823559 1101 6 0.6258572600705747 1101 7 0.590997469443316 1101 8 0.6431022261849506 1101 9 0.8529201799375201 1101 10-11 0.8198430885671684 1101 12-13 0.8393332182736231 1101 14-15 0.925498971108297 1101 16-17 0.8711442265831835 1101 18-19 0.927674153122581 1101 20-21 0.8839267425986606 1101 22-23 0.9121319614647945 1101 24-25 0.9462888488938219 1101 26-27 1.0795596822566011 1101 28-29 1.1780336744794226 1101 30-31 1.222106887788307 1101 32-33 1.0823249693389272 1101 34-35 1.255881288445643 1101 36-37 1.2733235367617013 1101 38-39 1.1714024337227045 1101 40-41 1.2060356096576683 1101 42-43 1.4468457801637342 1101 44-45 1.4538835825844316 1101 46-47 1.4842013646076069 1101 48-49 1.3317823206237662 1101 50-51 1.2921173123027145 1101 52-53 1.3386028104844883 1101 54-55 1.2923266970623253 1101 56-57 1.314793541205475 1101 58-59 1.2519653072496375 1101 60-61 1.4599073591798692 1101 62-63 1.4478649511667605 1101 64-65 1.31713666628006 1101 66-67 1.3461099308470743 1101 68-69 1.32728419693154 1101 70-71 1.3994717697902388 1101 72-73 1.524599394641161 1101 74-75 1.5077359974559137 1101 76-77 1.5084415131347413 1101 78-79 1.4043602387285326 1101 80-81 1.4956955533245129 1101 82-83 1.5159764493767156 1101 84-85 1.463965284639098 1101 86-87 1.4659631618332156 1101 88-89 1.5344147494831333 1101 90-91 1.5306672333461826 1101 92-93 1.646639783772084 1101 94-95 1.7570930885980793 1101 96-97 1.882008211522404 1101 98-99 1.9898514557545504 1101 100-101 2.2440262122541803 1102 1 0.523036318667355 1102 2 0.5728893412293843 1102 3 0.5719769721457304 1102 4 0.66055804084057 1102 5 0.5757222469567935 1102 6 0.5408320663824568 1102 7 0.47026076764513647 1102 8 0.6222358113972675 1102 9 0.6602891955384464 1102 10-11 0.7775060433601055 1102 12-13 0.715239568862728 1102 14-15 0.7423639662836194 1102 16-17 0.7867999851308767 1102 18-19 0.7386675890487879 1102 20-21 0.8966370680076636 1102 22-23 0.8546197864499874 1102 24-25 0.8442548265187426 1102 26-27 0.9471511159683743 1102 28-29 0.8365849608237568 1102 30-31 1.0200551849048551 1102 32-33 1.001116590187344 1102 34-35 1.0392307254447246 1102 36-37 1.0492380061328674 1102 38-39 1.0404577896435612 1102 40-41 1.1260277216282404 1102 42-43 1.1905275595954734 1102 44-45 1.1595983800546676 1102 46-47 1.244297547797025 1102 48-49 1.1458422598171865 1102 50-51 1.1978228352537386 1102 52-53 1.146306284178337 1102 54-55 1.065153843119166 1102 56-57 1.0784748388004317 1102 58-59 0.9081316988949446 1102 60-61 1.0886846358596678 1102 62-63 1.1473620850965425 1102 64-65 1.0339525806246748 1102 66-67 1.1828591517558777 1102 68-69 1.1567267557884655 1102 70-71 1.094089501881406 1102 72-73 1.1679269677779054 1102 74-75 1.278950021658055 1102 76-77 1.1964678522166103 1102 78-79 1.1015781425359883 1102 80-81 1.1354456314330008 1102 82-83 1.115924479696627 1102 84-85 1.1183376534371696 1102 86-87 1.0960155738714512 1102 88-89 1.2067184147115384 1102 90-91 1.3215050575062754 1102 92-93 1.4579957619078634 1102 94-95 1.4227966607121694 1102 96-97 1.5964974078770737 1102 98-99 1.7890989257857264 1102 100-101 2.0256640923476077 1103 1 0.3642212536553018 1103 2 0.4723311206273131 1103 3 0.4259118733562808 1103 4 0.5425572029146153 1103 5 0.4555505083397762 1103 6 0.40347604783561763 1103 7 0.31224900386722965 1103 8 0.40022799198748515 1103 9 0.4674037322673783 1103 10-11 0.5276775589769613 1103 12-13 0.5222628449945361 1103 14-15 0.6476148322097615 1103 16-17 0.5417206529235088 1103 18-19 0.557449549515141 1103 20-21 0.6427073739854379 1103 22-23 0.4833364556303792 1103 24-25 0.5771146651852561 1103 26-27 0.6970404728451811 1103 28-29 0.726137761944436 1103 30-31 0.7941556662308429 1103 32-33 0.7830218071892148 1103 34-35 0.7892670736990084 1103 36-37 0.8007597992173814 1103 38-39 0.8507755174853955 1103 40-41 0.8131345057319024 1103 42-43 0.9600979218817542 1103 44-45 0.8252746667193236 1103 46-47 0.8087020230056439 1103 48-49 1.0274930852566726 1103 50-51 0.921105289247528 1103 52-53 0.7776465077669883 1103 54-55 0.7234573076556678 1103 56-57 0.748276849714884 1103 58-59 0.7153505912212275 1103 60-61 0.6454443558546714 1103 62-63 0.7427968002040295 1103 64-65 0.7951906712996966 1103 66-67 0.7872052070873359 1103 68-69 0.7959838217350423 1103 70-71 0.8046549967560956 1103 72-73 0.8262832263483908 1103 74-75 0.7365321577360575 1103 76-77 0.7688954607445062 1103 78-79 0.8260298259907017 1103 80-81 0.9148229934436678 1103 82-83 0.8947058266236958 1103 84-85 0.9713838951952205 1103 86-87 1.0434349595095007 1103 88-89 1.1297189564593992 1103 90-91 1.0744550047950447 1103 92-93 1.0822975769365186 1103 94-95 1.1978796714591589 1103 96-97 1.262633589023757 1103 98-99 1.2688104182814435 1103 100-101 1.4705655597887386 1104 1 0.256332540228545 1104 2 0.24637383321197603 1104 3 0.3058205256429005 1104 4 0.41461117874297315 1104 5 0.29549767761096035 1104 6 0.27072411896563153 1104 7 0.2866327925294456 1104 8 0.29579653112116944 1104 9 0.4046438378916619 1104 10-11 0.46167108433134985 1104 12-13 0.4164349203666262 1104 14-15 0.34817228748627826 1104 16-17 0.502299207030859 1104 18-19 0.4180817151009961 1104 20-21 0.5060584817669991 1104 22-23 0.36950027922185313 1104 24-25 0.498004970783704 1104 26-27 0.42828197638318954 1104 28-29 0.40461044825315184 1104 30-31 0.46861493665743836 1104 32-33 0.5012195115992455 1104 34-35 0.5427355913620815 1104 36-37 0.6786695835910521 1104 38-39 0.6688937341415695 1104 40-41 0.6070506346262405 1104 42-43 0.654967308965368 1104 44-45 0.7202513139385047 1104 46-47 0.6198063004915113 1104 48-49 0.7843967156497698 1104 50-51 0.7171160951949886 1104 52-53 0.815752334669213 1104 54-55 0.9925852288445647 1104 56-57 0.885032537336933 1104 58-59 0.7933022107656242 1104 60-61 0.767837232815026 1104 62-63 0.8170033865712121 1104 64-65 0.9147637778190507 1104 66-67 0.6922722353774304 1104 68-69 0.7137988100842101 1104 70-71 0.7408936421925709 1104 72-73 0.7362930321926733 1104 74-75 0.7981353993344023 1104 76-77 0.8319094984379873 1104 78-79 0.7906013384794441 1104 80-81 0.7799755138244855 1104 82-83 0.7318517403750242 1104 84-85 0.8473342673990416 1104 86-87 0.8326949203418934 1104 88-89 0.7555673385880404 1104 90-91 0.737121739002756 1104 92-93 0.9885546443477473 1104 94-95 1.0579323070005557 1104 96-97 0.9949129784403183 1104 98-99 1.092591105694364 1104 100-101 1.2480099987596738 1105 1 -0.05661415942721959 1105 2 0.014570904359402448 1105 3 0.19015616010694458 1105 4 0.2213407724315104 1105 5 0.26524413426586335 1105 6 0.22353368890055947 1105 7 0.25823256792669724 1105 8 0.2373439766292762 1105 9 0.2532263131395922 1105 10-11 0.16215135713351714 1105 12-13 0.1396051161471732 1105 14-15 0.0336162672720377 1105 16-17 0.1282999119883712 1105 18-19 0.09780615321843555 1105 20-21 0.32941082345098494 1105 22-23 0.27714122177008704 1105 24-25 0.3816241352450973 1105 26-27 0.5188396806626159 1105 28-29 0.5123599260244518 1105 30-31 0.47336755713663337 1105 32-33 0.5079071903717818 1105 34-35 0.5507625345833986 1105 36-37 0.6421616689856293 1105 38-39 0.712648224944104 1105 40-41 0.5551800881429827 1105 42-43 0.6267347667965666 1105 44-45 0.5930798967780717 1105 46-47 0.6748058245996518 1105 48-49 0.8225794235154709 1105 50-51 0.7709445382270843 1105 52-53 0.6128184863707844 1105 54-55 0.5891792730464935 1105 56-57 0.5502961563829913 1105 58-59 0.6503970207208276 1105 60-61 0.5789030620941702 1105 62-63 0.5734162457614111 1105 64-65 0.6203171763017501 1105 66-67 0.657045476319432 1105 68-69 0.6490259881092086 1105 70-71 0.5121416538070669 1105 72-73 0.6032076089198775 1105 74-75 0.41624253830439173 1105 76-77 0.37259268333404094 1105 78-79 0.5089906927101246 1105 80-81 0.5480260740334586 1105 82-83 0.6287607248035663 1105 84-85 0.6927485779455793 1105 86-87 0.7901849209202467 1105 88-89 0.8623442573316709 1105 90-91 0.8666232775679852 1105 92-93 0.9076516930440093 1105 94-95 0.9983476581336141 1105 96-97 1.0026475327892186 1105 98-99 1.1278050817048602 1105 100-101 1.3313165500828994 1106 1 0.127913239953525 1106 2 0.18433889508714074 1106 3 0.23016957536939486 1106 4 0.3665539145824468 1106 5 0.3311274176158392 1106 6 0.2921347978902915 1106 7 0.24268734391444013 1106 8 0.18643720599983737 1106 9 0.13187645037292128 1106 10-11 0.2688937868047532 1106 12-13 0.3263484929779281 1106 14-15 0.29300226668595997 1106 16-17 0.2018445675965097 1106 18-19 0.06166207973937787 1106 20-21 0.19071305739230127 1106 22-23 0.3028547010281386 1106 24-25 0.3135135092536032 1106 26-27 0.231978863820828 1106 28-29 0.20854325982575972 1106 30-31 0.36196723743687187 1106 32-33 0.4140334369426597 1106 34-35 0.3335649752483363 1106 36-37 0.4134370816170687 1106 38-39 0.5955625901594956 1106 40-41 0.3320058880889505 1106 42-43 0.2284748724758643 1106 44-45 0.38054060239637266 1106 46-47 0.36636391845357963 1106 48-49 0.3615715435168596 1106 50-51 0.42416718164198386 1106 52-53 0.43430550025723846 1106 54-55 0.1509217645125709 1106 56-57 0.08769346356619323 1106 58-59 0.3949907174735934 1106 60-61 0.31808547316479974 1106 62-63 0.2640945942910342 1106 64-65 0.37096793289284946 1106 66-67 0.45389098881964074 1106 68-69 0.43186300768548946 1106 70-71 0.3952486807007922 1106 72-73 0.459218944189665 1106 74-75 0.5030341693170577 1106 76-77 0.4552572185123438 1106 78-79 0.4920185408984814 1106 80-81 0.4400031495568335 1106 82-83 0.4633597637384028 1106 84-85 0.4687059390355657 1106 86-87 0.5833958216891908 1106 88-89 0.572036372487041 1106 90-91 0.5222704515038039 1106 92-93 0.5285762645627408 1106 94-95 0.42152092403756924 1106 96-97 0.5000732852940892 1106 98-99 0.6502994698479405 1106 100-101 0.9922770413887463 1107 1 0.24956219518067613 1107 2 0.21586217478951042 1107 3 0.36218504881730595 1107 4 0.5380460322897065 1107 5 0.34092477901894824 1107 6 0.4015638188939832 1107 7 0.47269663976569376 1107 8 0.41931848832038554 1107 9 0.3262495147362472 1107 10-11 0.4079464320121815 1107 12-13 0.3823460124858542 1107 14-15 0.519853370713868 1107 16-17 0.41302983315849673 1107 18-19 0.47510391833134236 1107 20-21 0.3897497688119529 1107 22-23 0.4574591272781987 1107 24-25 0.6940654512781776 1107 26-27 0.45766271171412143 1107 28-29 0.38982419319225414 1107 30-31 0.5148786302321398 1107 32-33 0.6842745707762035 1107 34-35 0.5161697111317665 1107 36-37 0.5192830212070518 1107 38-39 0.6150002699369423 1107 40-41 0.4547391937470806 1107 42-43 0.43790284880946473 1107 44-45 0.646816374065061 1107 46-47 0.6024050366684506 1107 48-49 0.7515786232974548 1107 50-51 0.5671595175311595 1107 52-53 0.7753750437740905 1107 54-55 0.5398381402129253 1107 56-57 0.6236033986185419 1107 58-59 0.8655057432546833 1107 60-61 0.8246861783342361 1107 62-63 0.6418495092069385 1107 64-65 0.7699948312639187 1107 66-67 0.8612053235428974 1107 68-69 0.759532328156709 1107 70-71 0.6506478268799363 1107 72-73 0.69833310505242 1107 74-75 0.7358873654252669 1107 76-77 0.8276409878327478 1107 78-79 0.7214635265162883 1107 80-81 0.6815938806957469 1107 82-83 0.6947020757543001 1107 84-85 0.6932400504254836 1107 86-87 0.8282116720403678 1107 88-89 0.8140211840114304 1107 90-91 0.7934704212254964 1107 92-93 0.9017467903434842 1107 94-95 0.8513423847809243 1107 96-97 0.9607531531655837 1107 98-99 0.9898981007478405 1107 100-101 1.088139445664428 1108 1 0.17870801209252107 1108 2 0.1084023170254298 1108 3 0.17357410745641033 1108 4 0.3971387395251966 1108 5 0.22125810940010382 1108 6 0.31447014924857086 1108 7 0.32215255342040194 1108 8 0.21566693975558593 1108 9 0.22510377458102226 1108 10-11 0.30937868101065646 1108 12-13 0.15140372165502924 1108 14-15 0.3498796008124998 1108 16-17 0.2864967672924692 1108 18-19 0.23803881100492674 1108 20-21 0.33051985911906456 1108 22-23 0.2999690834974018 1108 24-25 0.4728575973046958 1108 26-27 0.46518301531607165 1108 28-29 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94-95 -0.0028904533338298677 2312 96-97 0.0999017472001924 2312 98-99 0.14111195548372635 2312 100-101 0.10005955609247863 2313 1 -0.5571512840139192 2313 2 -0.36142997637351115 2313 3 -0.16120904612814613 2313 4 0.04690278708843465 2313 5 -0.30807710926645626 2313 6 -0.22869424152642637 2313 7 -0.21016738683003666 2313 8 -0.09302152844372102 2313 9 0.055296848208520544 2313 10-11 -0.12599754985195943 2313 12-13 -0.24196859993202224 2313 14-15 -0.5538212299965579 2313 16-17 -0.31576201783534685 2313 18-19 -0.23922001500479695 2313 20-21 -0.22490487045181595 2313 22-23 -0.20394802971255643 2313 24-25 -0.5357800675371749 2313 26-27 -0.39829481927120014 2313 28-29 -0.42701813663089183 2313 30-31 -0.4954031050370773 2313 32-33 -0.8042205892617744 2313 34-35 -0.4309533158525909 2313 36-37 -0.32106851304255457 2313 38-39 -0.2737479899719091 2313 40-41 -0.17537970270155512 2313 42-43 -0.3226405085830919 2313 44-45 -0.300158864302027 2313 46-47 -0.2919180917007722 2313 48-49 -0.361149947530258 2313 50-51 -0.40099279355090545 2313 52-53 -0.3503512773995183 2313 54-55 -0.18678725890337589 2313 56-57 -0.2934114044237006 2313 58-59 -0.4422921531824642 2313 60-61 -0.4578379781557409 2313 62-63 -0.44604294563812275 2313 64-65 -0.3418412620347411 2313 66-67 -0.3377734160364412 2313 68-69 -0.4946923815865567 2313 70-71 -0.308516111011901 2313 72-73 -0.3354167317094259 2313 74-75 -0.3766213320768266 2313 76-77 -0.4753733612144515 2313 78-79 -0.4190791794989259 2313 80-81 -0.6056338227052933 2313 82-83 -0.45067161713932435 2313 84-85 -0.3886666720249181 2313 86-87 -0.49471649119998773 2313 88-89 -0.5183258482519904 2313 90-91 -0.4183348714555919 2313 92-93 -0.3793045838736617 2313 94-95 -0.3084184612818355 2313 96-97 -0.3015141487790771 2313 98-99 -0.3331329044983029 2313 100-101 -0.41729113129521167 2314 1 -0.7212586369378045 2314 2 -0.9234074721040741 2314 3 -0.564654550015181 2314 4 -0.8904929479873758 2314 5 -0.6775009927645215 2314 6 -0.584128521113982 2314 7 -0.4560122629803445 2314 8 -0.5412466449684246 2314 9 -0.5086559149450096 2314 10-11 -0.6510854801799155 2314 12-13 -0.6541754833334892 2314 14-15 -0.6720840119157927 2314 16-17 -0.8069000816608067 2314 18-19 -2.483931442471743 2314 20-21 -1.1323015120335214 2314 22-23 -0.8658060646369989 2314 24-25 -0.9352764283402166 2314 26-27 -1.0228162690018792 2314 28-29 -0.7450969119935138 2314 30-31 -0.7792418799114742 2314 32-33 -0.8268864668167879 2314 34-35 -0.8910047321526946 2314 36-37 -0.938027563834126 2314 38-39 -0.6991931156271747 2314 40-41 -0.5906300270862843 2314 42-43 -0.9312710364270345 2314 44-45 -0.8546941959276424 2314 46-47 -0.6853603104342412 2314 48-49 -0.6665632650992208 2314 50-51 -0.8054382114017429 2314 52-53 -0.7994073018457044 2314 54-55 -0.7820193913967266 2314 56-57 -0.758319217685866 2314 58-59 -0.7913382603945749 2314 60-61 -0.7708189491232886 2314 62-63 -0.8665039671497006 2314 64-65 -0.8981024603224128 2314 66-67 -0.8201107291142549 2314 68-69 -0.8351274977197889 2314 70-71 -0.9397365835173126 2314 72-73 -0.9874763309742782 2314 74-75 -0.949769626320009 2314 76-77 -0.9381359371975861 2314 78-79 -0.8557486790585465 2314 80-81 -0.8473013254214621 2314 82-83 -0.9776980634441799 2314 84-85 -0.8975034857802306 2314 86-87 -1.0031408261647847 2314 88-89 -0.9614480622162596 2314 90-91 -0.9397809292192214 2314 92-93 -0.9032499334760722 2314 94-95 -0.8016756984810876 2314 96-97 -0.927674405625396 2314 98-99 -1.0332182522519773 2314 100-101 -1.1164368611240079 2315 1 -1.029631047734398 2315 2 -1.8687754521014668 2315 3 -1.4147262017918258 2315 4 -0.6688217230047684 2315 5 -0.9482013438123928 2315 6 -1.485103901930188 2315 7 -1.3265304137961778 2315 8 -1.5408888444121374 2315 9 -1.615875007133802 2315 10-11 -0.9796739432355537 2315 12-13 -1.8161996139407677 2315 14-15 -8.778383073903903 2315 16-17 -4.426979720548076 2315 18-19 -1.9990300072056897 2315 20-21 -1.320705703219403 2315 22-23 -2.1815693485555556 2315 24-25 -2.019117806889845 2315 26-27 -1.6586404080423733 2315 28-29 -1.3888433695082938 2315 30-31 -1.638161708753863 2315 32-33 -1.9423498827029562 2315 34-35 -1.8605367467470444 2315 36-37 -1.5964669973053773 2315 38-39 -1.757784431004076 2315 40-41 -2.714291995087123 2315 42-43 -2.292017924463572 2315 44-45 -2.0039515764425673 2315 46-47 -1.6923121063577682 2315 48-49 -2.076918528052076 2315 50-51 -1.8213987668695388 2315 52-53 -1.7344409491533561 2315 54-55 -1.733330932099367 2315 56-57 -1.9280589689610075 2315 58-59 -2.08316473293025 2315 60-61 -1.7582876339358329 2315 62-63 -1.613909699230721 2315 64-65 -1.6117719464126488 2315 66-67 -1.6420882234287077 2315 68-69 -1.8696970584562038 2315 70-71 -1.6011226160957968 2315 72-73 -1.5762189507649005 2315 74-75 -1.719995738855161 2315 76-77 -1.6598930984794684 2315 78-79 -1.7256146382925088 2315 80-81 -1.7738088543579345 2315 82-83 -1.722982887658997 2315 84-85 -1.8012018556335612 2315 86-87 -1.8812162227779936 2315 88-89 -1.921747730112564 2315 90-91 -1.8716334973625024 2315 92-93 -1.8888851043235455 2315 94-95 -1.7846171750210083 2315 96-97 -1.9802389739593416 2315 98-99 -1.8685319984238014 2315 100-101 -2.1639659671756633 2316 1 -0.9592795265079168 2316 2 -1.061838787556038 2316 3 -1.4540322491893463 2316 4 -1.3931538033971336 2316 5 -0.8598637525956363 2316 6 -0.8901616408737354 2316 7 -1.0151681890278255 2316 8 -1.1853643073211089 2316 9 -1.4834004846325257 2316 10-11 -1.4084433689183413 2316 12-13 -1.1927232767820328 2316 14-15 -1.2072240286839815 2316 16-17 -1.926440779678451 2316 18-19 -1.369242467475054 2316 20-21 -1.759455184990344 2316 22-23 -1.2605692652981872 2316 24-25 -1.1341721508632006 2316 26-27 -1.2404667612957638 2316 28-29 -1.57898392619569 2316 30-31 -1.487509882995944 2316 32-33 -1.6014230706695542 2316 34-35 -2.0768890855300555 2316 36-37 -1.6833929753137582 2316 38-39 -1.6539835408789187 2316 40-41 -1.956196800514256 2316 42-43 -2.1213294727160488 2316 44-45 -2.496743203861172 2316 46-47 -2.2518586325454564 2316 48-49 -2.8923576432006257 2316 50-51 -2.302926876361415 2316 52-53 -3.0308172010072028 2316 54-55 -4.226880465080381 2316 56-57 -3.022220972105103 2316 58-59 -2.439690393443769 2316 60-61 -2.192414667088702 2316 62-63 -2.8159255688628555 2316 64-65 -2.853424148201146 2316 66-67 -2.616173907415824 2316 68-69 -2.6049096933483753 2316 70-71 -2.8197493814148338 2316 72-73 -6.007881483535069 2316 74-75 -3.6956925982096003 2316 76-77 -3.0328733065383595 2316 78-79 -2.9760682395621068 2316 80-81 -2.9160747078134044 2316 82-83 -2.9610194114934636 2316 84-85 -3.026009984291985 2316 86-87 -2.88058514353429 2316 88-89 -2.672253560479337 2316 90-91 -2.638722342132798 2316 92-93 -2.8712712711561217 2316 94-95 -3.0797846332426992 2316 96-97 -3.0676807281048823 2316 98-99 -3.457406148573103 2316 100-101 -3.610150941594764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 124885.0 3 20090.0 4 17444.0 5 16757.0 6 18519.0 7 22859.0 8 28062.0 9 33173.0 10 37903.0 11 45472.0 12 46959.0 13 52482.0 14 60043.0 15 71444.0 16 90391.0 17 119124.0 18 173763.0 19 255624.0 20 335318.0 21 244794.0 22 144768.0 23 129271.0 24 137591.0 25 149059.0 26 163796.0 27 184716.0 28 213102.0 29 248601.0 30 293440.0 31 350340.0 32 423561.0 33 524516.0 34 671672.0 35 922300.0 36 1316333.0 37 1461810.0 38 1050483.0 39 157308.0 40 170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.96784368936064 47.07841441485322 24.171927091140134 17.78181480464601 2 16.807781659176833 48.70375088858143 18.239599526965964 16.24886792527577 3 44.04733870525893 20.334956687001828 19.106826617846558 16.510877989892684 4 16.823464519612745 18.784951585071614 20.01991000931172 44.371673886003926 5 16.838175675414547 46.39096788355809 20.895600114704337 15.875256326323022 6 44.35316523046291 19.965432156984313 18.81057127390213 16.870831338650643 7 17.659318788332108 19.447152727344765 47.17151379243198 15.722014691891145 8 43.91027341302223 20.127490640395518 19.710860813054794 16.25137513352746 9 43.5252345615839 19.76101994505366 20.31272494539473 16.401020547967708 10-11 16.477378074413856 33.27692099144916 33.49367018161676 16.752030752520223 12-13 16.322736019426785 20.10847620066648 33.42177054935882 30.147017230547913 14-15 16.47928442670397 47.03782147324721 19.731270640766294 16.751623459282527 16-17 30.06522485642383 19.915064560913383 19.838439399099908 30.181271183562885 18-19 16.639014679268822 33.3286288729446 19.847162031855476 30.1851944159311 20-21 30.241761513291905 33.375854991293544 19.843685091865183 16.538698403549372 22-23 30.170916077976013 19.860109918326945 33.372579645101624 16.59639435859542 24-25 16.57717591189825 33.19771518227533 20.272097226647848 29.953011679178566 26-27 16.810091096555183 25.26881063527374 41.07004900057668 16.851049267594398 28-29 27.817376214132132 19.756228471408814 35.6968442102451 16.729551104213954 30-31 25.890392655532697 20.8160120041646 36.07679010863636 17.216805231666342 32-33 20.141612910064367 23.373639336181153 37.364807676492546 19.119940077261933 34-35 19.576014877735183 22.40671495639136 38.054620019548615 19.96265014632484 36-37 19.402509841352725 22.397977538085243 38.228179328858644 19.971333291703388 38-39 19.650698594118396 22.522808254950558 37.97615139053781 19.850341760393235 40-41 19.81189401271523 22.22817866229303 37.9848960778291 19.97503124716264 42-43 20.187561454785698 22.55824802910035 37.96276781729988 19.291422698814078 44-45 19.63499200382275 22.76991945963779 37.926009002720484 19.66907953381898 46-47 19.734243486297697 22.388061071001193 37.9755703052647 19.90212513743641 48-49 19.80302983841039 22.382968078789997 38.12156875906818 19.692433323731432 50-51 19.89263074616966 22.516659817889327 37.30856041469158 20.28214902124943 52-53 20.246631262218852 23.44804526381323 36.18759560427504 20.117727869692885 54-55 20.836353048801485 24.242780439226923 34.57940472516191 20.34146178680969 56-57 21.14991822421049 24.790641954073138 33.56574170305453 20.493698118661847 58-59 21.119252543750296 25.56719156280621 32.65063076449258 20.662925128950917 60-61 21.474452979884003 25.87182036551124 32.0454833360274 20.608243318577355 62-63 21.53314464437386 25.754637508842077 32.20643986319423 20.505777983589837 64-65 20.92891689436463 25.586410797519843 32.247633898766104 21.237038409349424 66-67 21.08506721424529 25.094350101318785 33.08683763688661 20.73374504754932 68-69 20.952902058980484 24.84145101741694 33.27493075962785 20.930716163974726 70-71 20.65022232991473 24.803128861227563 33.92316392901613 20.62348487984158 72-73 20.446610733600906 24.59247345226933 33.63896013484513 21.32195567928464 74-75 20.898786640498386 24.865712720121174 32.903298186968385 21.332202452412062 76-77 21.29291116542095 25.376529214907833 32.4955140453664 20.835045574304818 78-79 21.329568157558242 25.17802171606806 32.269421498060304 21.222988628313395 80-81 21.034039074207033 25.66236160338998 32.20310350711071 21.100495815292277 82-83 21.928249782729345 25.83215771830944 31.495851719321276 20.74374077963994 84-85 21.479471398657054 26.398864716381926 30.996381850916034 21.125282034044986 86-87 21.302202379949094 26.5303282853443 30.982055842784057 21.185413491922553 88-89 21.285472479445605 26.05551195837207 31.000321143560832 21.658694418621497 90-91 21.234088123969986 25.930821657319818 31.068795614930007 21.766294603780192 92-93 21.16916675351157 26.45779912450681 30.998578576735845 21.374455545245773 94-95 21.264534649975797 25.925952576666685 30.64588265184823 22.163630121509296 96-97 21.253136511052904 25.818573553523066 31.08968906621844 21.83860086920559 98-99 21.678620770248937 26.181756458861805 30.452662377579692 21.686960393309565 100-101 21.927137715911787 25.940229489904137 30.263812717233566 21.868820076950506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10339.0 1 6486.0 2 1926.5 3 1091.5 4 918.5 5 852.5 6 997.0 7 1202.5 8 1141.0 9 1184.5 10 1607.0 11 10590.5 12 194439.0 13 220339.0 14 63246.5 15 56479.5 16 59467.0 17 62673.0 18 65208.5 19 66416.0 20 66765.5 21 68059.0 22 69783.0 23 72648.5 24 77203.0 25 82712.5 26 90686.0 27 99132.5 28 106932.0 29 115808.5 30 125881.5 31 136628.5 32 147528.0 33 160574.5 34 183306.5 35 201790.5 36 212556.5 37 226775.0 38 236296.5 39 243286.0 40 255021.5 41 266771.5 42 273257.0 43 285295.5 44 302355.5 45 312525.0 46 317205.5 47 324833.5 48 334668.5 49 342885.0 50 347246.0 51 344013.0 52 342525.5 53 350575.0 54 336659.5 55 307937.5 56 296590.5 57 277324.5 58 266940.5 59 232438.5 60 171134.0 61 134645.5 62 103196.5 63 77425.5 64 57623.0 65 40760.5 66 27392.0 67 17906.0 68 11594.0 69 7476.0 70 5303.0 71 4101.5 72 3553.5 73 3156.5 74 2851.5 75 2697.5 76 2573.0 77 2426.5 78 2286.0 79 2184.5 80 2085.0 81 1931.0 82 1704.5 83 1519.5 84 1377.0 85 1224.0 86 1103.0 87 948.0 88 728.0 89 557.0 90 454.5 91 370.5 92 279.5 93 180.0 94 123.0 95 89.5 96 56.5 97 28.0 98 21.0 99 14.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10034810965845245 2 0.08285428873281114 3 0.08884003319964205 4 0.19723028018207864 5 0.07583552062412392 6 0.09265353169060692 7 0.09710422233449248 8 0.08638780885355327 9 0.07925318762615319 10-11 0.07686854426598022 12-13 0.07033732469854295 14-15 0.0770037062378119 16-17 0.06665898817940975 18-19 0.058554097082789504 20-21 0.05378963757572329 22-23 0.043720070674264186 24-25 0.04568474647910304 26-27 0.045351668762803575 28-29 0.04402418511088543 30-31 0.034142879527334725 32-33 0.038072231137012434 34-35 0.05109122535236967 36-37 0.046519854376491546 38-39 0.05041541549321134 40-41 0.048301096076701716 42-43 0.04637021076482078 44-45 0.050043720070674265 46-47 0.043623526408670135 48-49 0.03949143184124492 50-51 0.04357525427587312 52-53 0.03924524396398011 54-55 0.043024951961987044 56-57 0.04178918536238325 58-59 0.04894311544290213 60-61 0.047929400654164636 62-63 0.05267455130811205 64-65 0.04872589084531552 66-67 0.04328079426581127 68-69 0.04361869919539044 70-71 0.052351128018371995 72-73 0.03995967152937605 74-75 0.04780872032217207 76-77 0.04629780256562524 78-79 0.04481102087547691 80-81 0.04432829954750668 82-83 0.04341595623764294 84-85 0.04424623692175174 86-87 0.048991387575699145 88-89 0.0416878138835095 90-91 0.044704822183323466 92-93 0.04089615090563831 94-95 0.043806960513298826 96-97 0.029788733149043206 98-99 0.03628133501024286 100-101 0.024961519869340854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0357943E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.897485519889706 #Duplication Level Percentage of deduplicated Percentage of total 1 91.93890350012039 43.11703395611433 2 4.465481223860981 4.188396820707195 3 1.1552732236917627 1.6253822783880219 4 0.5661969573673118 1.0621285443815645 5 0.35327256847068084 0.8283797582213999 6 0.22796819239287508 0.6414681001044173 7 0.17726589863697848 0.5819327440148553 8 0.13600531056704515 0.5102645666356881 9 0.10460468779373211 0.4415127148007221 >10 0.8345038367071025 7.411169522160545 >50 0.023634053651463455 0.7596829182022217 >100 0.009405608511655337 0.9845549941594851 >500 0.0032298021121649886 1.0460538508667936 >1k 0.003537402313160047 3.311534134964858 >5k 5.639337020979786E-4 1.8063831461006985 >10k+ 1.5380010057217596E-4 31.684121950177218 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1276982 12.328528936681733 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 29936 0.2890149134823391 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 10904 0.10527186720374886 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.93088531188094E-5 0.0 0.0 0.0 9.6544265594047E-6 2 6.75809859158329E-5 0.0 0.0 0.0 9.6544265594047E-6 3 8.68898390346423E-5 0.0 0.0 0.0 9.6544265594047E-6 4 9.6544265594047E-5 0.0 0.0 0.0 9.6544265594047E-6 5 9.6544265594047E-5 0.0 0.0 0.0 9.6544265594047E-6 6 1.158531187128564E-4 0.0 0.0 0.0 9.6544265594047E-6 7 1.2550754527226112E-4 0.0 0.0 9.6544265594047E-6 9.6544265594047E-6 8 1.4481639839107052E-4 0.0 0.0 9.6544265594047E-6 9.6544265594047E-6 9 1.8343410462868932E-4 0.0 0.0 9.6544265594047E-6 9.6544265594047E-6 10-11 2.1239738430690343E-4 0.0 0.0 1.93088531188094E-5 9.6544265594047E-6 12-13 2.4136066398511752E-4 0.0 0.0 1.93088531188094E-5 2.413606639851175E-5 14-15 2.654967303836293E-4 0.0 0.0 1.93088531188094E-5 2.89632796782141E-5 16-17 2.9446001006184336E-4 0.0 0.0 1.93088531188094E-5 3.379049295791645E-5 18-19 3.2342328974005745E-4 0.0 0.0 1.93088531188094E-5 3.86177062376188E-5 20-21 3.86177062376188E-4 0.0 0.0 1.93088531188094E-5 3.86177062376188E-5 22-23 4.151403420544021E-4 0.0 0.0 1.93088531188094E-5 3.86177062376188E-5 24-25 4.634124748514256E-4 9.6544265594047E-6 0.0 1.93088531188094E-5 3.86177062376188E-5 26-27 4.923757545296397E-4 9.6544265594047E-6 0.0 2.89632796782141E-5 4.82721327970235E-5 28-29 5.503023138860679E-4 9.6544265594047E-6 0.0 2.89632796782141E-5 4.82721327970235E-5 30-31 5.937472334033891E-4 9.6544265594047E-6 0.0 2.89632796782141E-5 5.309934607672585E-5 32-33 6.806370724380314E-4 9.6544265594047E-6 0.0 2.89632796782141E-5 5.79265593564282E-5 34-35 7.433908450741619E-4 9.6544265594047E-6 0.0 2.89632796782141E-5 6.275377263613056E-5 36-37 7.916629778711853E-4 9.6544265594047E-6 0.0 2.89632796782141E-5 9.6544265594047E-5 38-39 8.39935110668209E-4 9.6544265594047E-6 0.0 2.89632796782141E-5 9.6544265594047E-5 40-41 8.930344567449349E-4 9.6544265594047E-6 0.0 2.89632796782141E-5 1.061986921534517E-4 42-43 9.36479376262256E-4 9.6544265594047E-6 0.0 3.379049295791645E-5 1.1102590543315405E-4 44-45 9.702698692201724E-4 9.6544265594047E-6 0.0 3.86177062376188E-5 1.158531187128564E-4 46-47 0.0010185420020171958 9.6544265594047E-6 0.0 3.86177062376188E-5 1.2550754527226112E-4 48-49 0.0010957774144924335 9.6544265594047E-6 0.0 3.86177062376188E-5 1.2550754527226112E-4 50-51 0.0012261121730443969 9.6544265594047E-6 0.0 3.86177062376188E-5 1.2550754527226112E-4 52-53 0.0013612741448760627 9.6544265594047E-6 0.0 3.86177062376188E-5 1.351619718316658E-4 54-55 0.0014723000503092168 9.6544265594047E-6 0.0 3.86177062376188E-5 1.4481639839107052E-4 56-57 0.0015688443159032638 9.6544265594047E-6 0.0 3.86177062376188E-5 1.4481639839107052E-4 58-59 0.0016750430080567156 9.6544265594047E-6 0.0 3.86177062376188E-5 1.5929803823017756E-4 60-61 0.0017860689134898695 9.6544265594047E-6 0.0 3.86177062376188E-5 1.6412525150987992E-4 62-63 0.0019501941649997497 9.6544265594047E-6 0.0 3.86177062376188E-5 1.6412525150987992E-4 64-65 0.0020370840040343915 9.6544265594047E-6 0.0 3.86177062376188E-5 1.6895246478958225E-4 66-67 0.0021577643360269506 9.6544265594047E-6 0.0 4.82721327970235E-5 1.7860689134898696E-4 68-69 0.002331544014096235 9.6544265594047E-6 0.0 4.82721327970235E-5 1.93088531188094E-4 70-71 0.0025584230382422455 9.6544265594047E-6 0.0 4.82721327970235E-5 1.9791574446779636E-4 72-73 0.0027804748491085537 9.6544265594047E-6 0.0 4.82721327970235E-5 2.0757017102720105E-4 74-75 0.0030218355130936713 9.6544265594047E-6 0.0 4.82721327970235E-5 2.3653345070541516E-4 76-77 0.0032631961770787886 9.6544265594047E-6 0.0 5.309934607672585E-5 2.4136066398511752E-4 78-79 0.0035480017605812276 9.6544265594047E-6 0.0 5.79265593564282E-5 2.5584230382422456E-4 80-81 0.0040017598088732485 1.448163983910705E-5 0.0 5.79265593564282E-5 2.606695171039269E-4 82-83 0.004532753269640507 1.93088531188094E-5 0.0 6.275377263613056E-5 2.7515115694303394E-4 84-85 0.005261662474875562 1.93088531188094E-5 0.0 6.75809859158329E-5 2.8963279678214103E-4 86-87 0.00620296906441752 1.93088531188094E-5 0.0 6.75809859158329E-5 2.9928722334154575E-4 88-89 0.0076463058350485225 1.93088531188094E-5 0.0 7.72354124752376E-5 3.089416499009504E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 280315 0.0 91.75416 5 GGTATCA 281585 0.0 91.48275 8 AAGCAGT 282465 0.0 91.415276 1 TGGTATC 282015 0.0 91.27992 7 GTATCAA 282200 0.0 91.229996 9 GCAGTGG 283965 0.0 90.79535 3 GTGGTAT 284045 0.0 90.68266 6 AGCAGTG 285600 0.0 90.40209 2 CAGTGGT 284650 0.0 90.233025 4 TCAACGC 280985 0.0 46.35087 12-13 AACGCAG 281930 0.0 46.237755 14-15 CAACGCA 281175 0.0 46.18606 12-13 ATCAACG 281145 0.0 46.179974 10-11 CGCAGAG 283495 0.0 46.010315 16-17 ACGCAGA 282475 0.0 46.004745 14-15 TATCAAC 282890 0.0 45.934586 10-11 CAGAGTA 281400 0.0 45.75424 18-19 AGAGTAC 276500 0.0 45.641758 18-19 GAGTACT 160100 0.0 45.44061 20-21 GCAGAGT 285375 0.0 45.367653 16-17 >>END_MODULE