FastQCFastQC Report
Sat 18 Jun 2016
SRR578562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357943
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1258331.2148454572495717No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121660.11745575352171758No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113410.1094908516102087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4150.024.0354923
CGCGTAG2850.019.99993954-55
CAACGCA75050.019.33454512-13
ATCAACG74500.019.1904310-11
CTCGCGT2950.018.51751952-53
ACGTACG3250.018.26846920-21
AGTGGTA93950.017.846695
GGTATCA96500.017.4733668
GTATCAA100550.016.7223289
TCAACGC73650.016.54204210-11
TCGCGTA3100.016.08920552-53
TGGTATC109300.015.3836237
AAGCAGT115450.015.2453681
AACGCAG79750.015.18738312-13
ACTCGCG3350.014.88873350-51
ACGCAGA100400.014.54738214-15
CGTACGC3750.013.93275420-21
CGCTAGT3650.013.66391490-91
AGAGTAC109400.012.74284318-19
GTGGTAT136600.012.3440426
Produced by FastQC (version 0.11.5)