##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578562_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10357943 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.491536881405892 31.0 31.0 34.0 30.0 34.0 2 31.811622346251568 31.0 31.0 34.0 30.0 34.0 3 31.88237143224287 31.0 31.0 34.0 30.0 34.0 4 35.55099752914261 37.0 35.0 37.0 33.0 37.0 5 35.3706433796749 37.0 35.0 37.0 33.0 37.0 6 35.36685092783384 37.0 35.0 37.0 32.0 37.0 7 35.34340293241621 37.0 35.0 37.0 32.0 37.0 8 35.32848278852278 37.0 35.0 37.0 32.0 37.0 9 36.89645405463228 39.0 37.0 39.0 33.0 39.0 10-11 36.863376299715114 39.0 37.0 39.0 32.5 39.0 12-13 36.84395704822859 39.0 37.0 39.0 32.0 39.0 14-15 38.02604325009319 40.0 37.0 41.0 33.0 41.0 16-17 37.97829684909446 40.0 37.0 41.0 33.0 41.0 18-19 37.92058379738139 40.0 37.0 41.0 33.0 41.0 20-21 37.87237157030117 40.0 37.0 41.0 32.0 41.0 22-23 37.78222360366339 40.0 37.0 41.0 32.0 41.0 24-25 37.694096984314356 39.5 37.0 41.0 32.0 41.0 26-27 37.510395838247035 39.0 36.0 41.0 32.0 41.0 28-29 37.33878386857313 39.0 36.0 41.0 31.5 41.0 30-31 37.20877924313737 39.0 36.0 41.0 31.0 41.0 32-33 37.07802210342343 39.0 36.0 41.0 31.0 41.0 34-35 36.92290114938845 39.0 36.0 40.0 30.0 41.0 36-37 36.77480465957382 39.0 35.0 40.0 30.0 41.0 38-39 36.60726304440949 38.5 35.0 40.0 30.0 41.0 40-41 36.43729044463751 38.0 35.0 40.0 30.0 41.0 42-43 36.37670631128208 38.0 35.0 40.0 30.0 41.0 44-45 36.410599092889385 38.0 35.0 40.0 30.0 41.0 46-47 36.276262767617084 38.0 35.0 40.0 30.0 41.0 48-49 36.129924541967455 38.0 35.0 40.0 29.0 41.0 50-51 35.926718220017236 38.0 34.5 40.0 28.5 41.0 52-53 35.991985039886785 38.0 35.0 40.0 29.0 41.0 54-55 35.97663406720813 38.0 35.0 40.0 28.5 41.0 56-57 35.80689915941804 38.0 34.0 40.0 28.0 41.0 58-59 35.58533335238474 38.0 34.0 40.0 28.0 41.0 60-61 35.31341044259463 38.0 34.0 40.0 28.0 41.0 62-63 35.01799700963792 37.0 34.0 40.0 27.5 41.0 64-65 34.67762822212866 37.0 33.0 39.5 26.5 41.0 66-67 34.31804471215955 36.0 33.0 39.0 26.0 41.0 68-69 33.939047115822135 36.0 32.5 39.0 26.0 40.5 70-71 33.54074307997254 35.0 32.0 38.5 26.0 40.0 72-73 33.136133014055005 35.0 32.0 38.0 26.0 40.0 74-75 32.72142050791359 35.0 31.5 37.0 25.0 39.0 76-77 31.367994398115535 34.0 29.5 36.0 23.0 38.5 78-79 32.009728089834056 34.5 31.0 36.0 25.0 39.0 80-81 31.893238744410933 35.0 31.0 36.0 24.5 37.5 82-83 31.59056807900951 34.0 31.0 35.5 24.0 37.0 84-85 31.275620120713157 34.0 31.0 35.0 23.0 37.0 86-87 30.939682521906136 34.0 31.0 35.0 22.0 36.0 88-89 30.62611780157508 34.0 30.5 35.0 20.0 36.0 90-91 30.307630047780723 34.0 30.0 35.0 19.5 36.0 92-93 29.952718459640103 34.0 30.0 35.0 18.0 35.0 94-95 29.58657650462066 34.0 30.0 35.0 13.0 35.0 96-97 29.1936550529386 34.0 29.0 35.0 4.5 35.0 98-99 28.710216787252065 34.0 29.0 35.0 2.0 35.0 100-101 27.574650536308223 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3765266964598055 1101 2 0.36296719874329497 1101 3 0.34735512110827216 1101 4 0.26126541036033757 1101 5 0.2691493555836644 1101 6 0.2478959025380334 1101 7 0.29430420750417596 1101 8 0.2883296312157171 1101 9 0.347929728118352 1101 10-11 0.34429445517884716 1101 12-13 0.32695321143764033 1101 14-15 0.43838729187358894 1101 16-17 0.4682412682237711 1101 18-19 0.35697898817720386 1101 20-21 0.381247874044945 1101 22-23 0.465483720899023 1101 24-25 0.48183069769590503 1101 26-27 0.5040301533527014 1101 28-29 0.5438775508550577 1101 30-31 0.5684691757309679 1101 32-33 0.5856244123965197 1101 34-35 0.6167751008396891 1101 36-37 0.5604186395478123 1101 38-39 0.5000411408495609 1101 40-41 0.5722578519387227 1101 42-43 0.552554373440195 1101 44-45 0.5061993328854086 1101 46-47 0.5434375040692672 1101 48-49 0.4999300497632291 1101 50-51 0.4548389239252302 1101 52-53 0.447829153884463 1101 54-55 0.4662776314755135 1101 56-57 0.5886854864060851 1101 58-59 0.5177064016763424 1101 60-61 0.5645841194537056 1101 62-63 0.5158969677121803 1101 64-65 0.5203628921188042 1101 66-67 0.4645667166778509 1101 68-69 0.5219386588735873 1101 70-71 0.5195035479373047 1101 72-73 0.5537076163134884 1101 74-75 0.428072253470539 1101 76-77 0.32388674015870933 1101 78-79 0.5329880740849475 1101 80-81 0.5414889023848524 1101 82-83 0.48585367133757984 1101 84-85 0.5800296120933943 1101 86-87 0.5150370357281737 1101 88-89 0.5105336831909568 1101 90-91 0.6187202874898929 1101 92-93 0.6233047671157443 1101 94-95 0.6454301724964857 1101 96-97 0.5312234447406077 1101 98-99 0.7078053295117392 1101 100-101 0.8970773126669442 1102 1 0.2884085093378488 1102 2 0.2668610420258908 1102 3 0.3452369934411479 1102 4 0.27669616928900354 1102 5 0.33730111257653306 1102 6 0.221398610022554 1102 7 0.262763528754256 1102 8 0.22756135677096268 1102 9 0.45552904433253616 1102 10-11 0.31594671497165905 1102 12-13 0.25787068138275515 1102 14-15 0.5157898216805563 1102 16-17 0.46369609747589635 1102 18-19 0.4922792857987588 1102 20-21 0.4557877781450159 1102 22-23 0.3984660667623743 1102 24-25 0.41844956408731804 1102 26-27 0.436835472064935 1102 28-29 0.4052354900922239 1102 30-31 0.3651056124853582 1102 32-33 0.4400957225591071 1102 34-35 0.4669191524708012 1102 36-37 0.49335126700045606 1102 38-39 0.5214006783794147 1102 40-41 0.42888608740069856 1102 42-43 0.4245613300500892 1102 44-45 0.3794251776631441 1102 46-47 0.4237149394137063 1102 48-49 0.4732135767800756 1102 50-51 0.35954259252511633 1102 52-53 0.5041177773172336 1102 54-55 0.47280355409721864 1102 56-57 0.5043218856162994 1102 58-59 0.5736454170885708 1102 60-61 0.5043675195072623 1102 62-63 0.4293106998197658 1102 64-65 0.5320551719999216 1102 66-67 0.49194260136454204 1102 68-69 0.4701339550806267 1102 70-71 0.467125678462061 1102 72-73 0.4561421169091062 1102 74-75 0.3919961502616971 1102 76-77 0.3720272587674778 1102 78-79 0.4374828562722186 1102 80-81 0.4687183420479535 1102 82-83 0.4346854618983471 1102 84-85 0.3169472893408347 1102 86-87 0.3717931474359837 1102 88-89 0.5063599373463816 1102 90-91 0.5721306131831341 1102 92-93 0.4493776168899686 1102 94-95 0.41000761545241815 1102 96-97 0.3498318955617137 1102 98-99 0.42818719138277217 1102 100-101 0.591747329280345 1103 1 0.1837740462551949 1103 2 0.22996551301330115 1103 3 0.2703850093098197 1103 4 0.1273762311651936 1103 5 0.17794971101866253 1103 6 0.13036336244154967 1103 7 0.14630674541319166 1103 8 0.10048171667702377 1103 9 0.3100992263699851 1103 10-11 0.26871365578087136 1103 12-13 0.2467805930962612 1103 14-15 0.37455920439919055 1103 16-17 0.26327566516217615 1103 18-19 0.26312439821317213 1103 20-21 0.2947041969181896 1103 22-23 0.3073073410322209 1103 24-25 0.2931110101882197 1103 26-27 0.250729819985537 1103 28-29 0.2569098283749014 1103 30-31 0.2530074133097884 1103 32-33 0.2359469703662711 1103 34-35 0.20405541380510073 1103 36-37 0.3644037695551319 1103 38-39 0.3286182323196485 1103 40-41 0.28083487185345035 1103 42-43 0.29880507452997307 1103 44-45 0.1885273524807829 1103 46-47 0.30212385683155674 1103 48-49 0.21767255260932217 1103 50-51 0.26672174537719684 1103 52-53 0.33137623734212696 1103 54-55 0.3539539964694427 1103 56-57 0.36644705471510974 1103 58-59 0.3783707999131991 1103 60-61 0.42723544549185277 1103 62-63 0.3320435031188467 1103 64-65 0.4054511115022521 1103 66-67 0.2636100027634285 1103 68-69 0.3277578237688914 1103 70-71 0.25058864202699027 1103 72-73 0.30905737015022794 1103 74-75 0.3243110404940168 1103 76-77 0.33388077208671163 1103 78-79 0.3303133070622053 1103 80-81 0.38598813618068206 1103 82-83 0.31466087834631296 1103 84-85 0.406375260446179 1103 86-87 0.3894862836685107 1103 88-89 0.320674865862312 1103 90-91 0.36795424594571813 1103 92-93 0.27450097564352305 1103 94-95 0.3515459787191233 1103 96-97 0.40251043356502336 1103 98-99 0.43367642290191455 1103 100-101 0.6361662632949461 1104 1 0.1652616904266644 1104 2 0.17113063320692845 1104 3 0.16947101167623657 1104 4 0.1252683371352319 1104 5 0.16126856640244114 1104 6 0.2452838607457437 1104 7 0.07266727145258756 1104 8 0.10158512046682233 1104 9 0.260443453330204 1104 10-11 0.19583918638858222 1104 12-13 0.22756330395212387 1104 14-15 0.21563572809262155 1104 16-17 0.1645501925530155 1104 18-19 0.20262942150089458 1104 20-21 0.2278100377623673 1104 22-23 0.2705105711052127 1104 24-25 0.26678983765091147 1104 26-27 0.26655523506087775 1104 28-29 0.25369326978939455 1104 30-31 0.23038698499423305 1104 32-33 0.1788453176864877 1104 34-35 0.22942089295769108 1104 36-37 0.3179727436779345 1104 38-39 0.35020019848901995 1104 40-41 0.32758328177306595 1104 42-43 0.20862226756754154 1104 44-45 0.2838055177933754 1104 46-47 0.24460982889744542 1104 48-49 0.16933615359418042 1104 50-51 0.2288463046951179 1104 52-53 0.23695277916009871 1104 54-55 0.23904478185715305 1104 56-57 0.245422804072156 1104 58-59 0.24861996130438513 1104 60-61 0.3371569708536697 1104 62-63 0.23673995414678473 1104 64-65 0.29084593383058177 1104 66-67 0.2613663140557634 1104 68-69 0.2486555929625638 1104 70-71 0.10538187813219935 1104 72-73 0.25515287814408794 1104 74-75 0.24869549837024607 1104 76-77 0.2289971920891425 1104 78-79 0.2142403850414425 1104 80-81 0.36085208590040097 1104 82-83 0.19436780907987483 1104 84-85 0.24793605342761182 1104 86-87 0.3163568902087768 1104 88-89 0.28462271736000133 1104 90-91 0.3294985409426907 1104 92-93 0.3567678172972606 1104 94-95 0.32218643512308276 1104 96-97 0.4902107539512315 1104 98-99 0.517018477704962 1104 100-101 0.5443759670840933 1105 1 0.15002031761753187 1105 2 0.14731846157800987 1105 3 0.1129692950500285 1105 4 0.12723952222437873 1105 5 0.16997810705521488 1105 6 0.22683332822502678 1105 7 0.2278443146112039 1105 8 0.1641653469549169 1105 9 0.2122188943043568 1105 10-11 0.1397449106163151 1105 12-13 0.16193203680919765 1105 14-15 0.2335987606304144 1105 16-17 0.15637940247687965 1105 18-19 0.2847918513637637 1105 20-21 0.23840064461330002 1105 22-23 0.20167557715974027 1105 24-25 0.22236812368422676 1105 26-27 0.2704061930152619 1105 28-29 0.2562739607843838 1105 30-31 0.2098490677975846 1105 32-33 0.22879425380632057 1105 34-35 0.2464382759896182 1105 36-37 0.28596017648951744 1105 38-39 0.3299157517736475 1105 40-41 0.3483306642539574 1105 42-43 0.24669278335690592 1105 44-45 0.19963168200931136 1105 46-47 0.183149888290572 1105 48-49 0.27310690213793265 1105 50-51 0.2923186030069118 1105 52-53 0.2297463782551361 1105 54-55 0.2788976880080938 1105 56-57 0.2970637338692299 1105 58-59 0.21221594140708788 1105 60-61 0.12753863612909555 1105 62-63 0.1979342605674077 1105 64-65 0.2526269671033887 1105 66-67 0.18034949480768603 1105 68-69 0.14753344905376764 1105 70-71 0.1882366919491858 1105 72-73 0.23956491488384302 1105 74-75 0.29826896719385587 1105 76-77 0.21655164982972153 1105 78-79 0.25465564509470795 1105 80-81 0.21883345279846012 1105 82-83 0.2302908457526911 1105 84-85 0.3117960738203749 1105 86-87 0.28091967309688926 1105 88-89 0.31675576943897 1105 90-91 0.3042202626056074 1105 92-93 0.2615856554548621 1105 94-95 0.3170360920027697 1105 96-97 0.39182360385129655 1105 98-99 0.5032627436633952 1105 100-101 0.6081236451188836 1106 1 -0.04133507741357789 1106 2 0.018017536133413614 1106 3 -0.048600119365794114 1106 4 0.04814853120027607 1106 5 0.011617127758043466 1106 6 0.04572428573536058 1106 7 0.0656494352208199 1106 8 0.054005960714285095 1106 9 -0.06453867835526239 1106 10-11 -0.041999326825049366 1106 12-13 -0.05155497270463627 1106 14-15 0.16825741532493055 1106 16-17 0.10080335404158092 1106 18-19 -0.0016264367218283837 1106 20-21 0.069578385981508 1106 22-23 0.003098705118460998 1106 24-25 0.1240749143554396 1106 26-27 0.15183131611618705 1106 28-29 0.09031081121625562 1106 30-31 0.061418668501374896 1106 32-33 0.07560681267177216 1106 34-35 0.08688670253938113 1106 36-37 0.06623061420260967 1106 38-39 0.1021714166892096 1106 40-41 0.003212594007827363 1106 42-43 -0.03097348966578295 1106 44-45 0.025339581663018862 1106 46-47 0.021317362464039036 1106 48-49 0.07473987056008724 1106 50-51 0.00885138446533773 1106 52-53 -0.10807825199760401 1106 54-55 -0.11301388859765638 1106 56-57 -0.06518921912011422 1106 58-59 0.04573772641936813 1106 60-61 0.11787026862267425 1106 62-63 -0.029614205650311476 1106 64-65 -0.0343561429933672 1106 66-67 -0.06206669268696885 1106 68-69 0.019479790136067265 1106 70-71 -0.06074196310091651 1106 72-73 -0.1592177917974027 1106 74-75 -0.07007500117614285 1106 76-77 -0.1661052142960351 1106 78-79 -0.11823497807301209 1106 80-81 0.03318569669019311 1106 82-83 0.07242630307951003 1106 84-85 0.1782329169814183 1106 86-87 0.30739891149390175 1106 88-89 0.20786161449733243 1106 90-91 0.2797063124564083 1106 92-93 0.1502306610060984 1106 94-95 0.3400855134567067 1106 96-97 0.34721618484942596 1106 98-99 0.3177844535822949 1106 100-101 0.2746430114955416 1107 1 0.09323836494906246 1107 2 0.1235391484738102 1107 3 0.08841247353277737 1107 4 0.16859882168706264 1107 5 0.042373893623839365 1107 6 0.042863259739171156 1107 7 0.0801164034375006 1107 8 0.0887199368930709 1107 9 0.022506316441933905 1107 10-11 0.030196915650748224 1107 12-13 0.020549647066275156 1107 14-15 0.07901654111759626 1107 16-17 0.1798910967389915 1107 18-19 0.05524084770450344 1107 20-21 -0.017978599915785765 1107 22-23 -0.08098879439345552 1107 24-25 -0.016414135104376726 1107 26-27 0.054440662248616434 1107 28-29 0.04798478646340243 1107 30-31 0.12084827178019708 1107 32-33 0.09071019048408147 1107 34-35 0.0830667721520868 1107 36-37 0.11258600000784469 1107 38-39 0.16348156314167994 1107 40-41 0.05166951352575211 1107 42-43 0.15755647798027184 1107 44-45 0.10814809671757075 1107 46-47 0.14290733664527266 1107 48-49 0.1409089831248238 1107 50-51 0.17221334860951742 1107 52-53 0.13506295794272205 1107 54-55 0.11431981876182107 1107 56-57 0.19826001779119906 1107 58-59 0.20683696939319418 1107 60-61 0.3682437809953356 1107 62-63 0.3168206264592186 1107 64-65 0.31901110272879407 1107 66-67 0.2354858679193086 1107 68-69 0.3848970142455457 1107 70-71 0.34491139755139244 1107 72-73 0.21733573538560336 1107 74-75 0.17376697688784049 1107 76-77 0.2633736974111862 1107 78-79 0.269018180084565 1107 80-81 0.42097971994421 1107 82-83 0.45940885743146964 1107 84-85 0.42085838528475605 1107 86-87 0.4247006690742694 1107 88-89 0.4997269568648335 1107 90-91 0.4840567639936495 1107 92-93 0.5299785666994481 1107 94-95 0.4111092935731726 1107 96-97 0.43570700305559384 1107 98-99 0.3762839237119202 1107 100-101 0.6464343843701528 1108 1 0.012383975208472009 1108 2 0.00627938931246419 1108 3 -0.020150908245792465 1108 4 0.005870360037327771 1108 5 -0.07408833473891008 1108 6 -0.04842988264730508 1108 7 0.003975312962111843 1108 8 -0.006915711242776013 1108 9 -0.12617715992440282 1108 10-11 -0.1458466791569606 1108 12-13 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-1.4096436380082906 2316 100-101 -1.704749222316707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 170.0 3 94.0 4 266.0 5 623.0 6 1166.0 7 2207.0 8 4084.0 9 6520.0 10 8957.0 11 11484.0 12 14704.0 13 17791.0 14 20787.0 15 24048.0 16 27980.0 17 32397.0 18 38086.0 19 43892.0 20 47390.0 21 50072.0 22 56150.0 23 66231.0 24 81603.0 25 103526.0 26 131326.0 27 166950.0 28 210116.0 29 260733.0 30 317998.0 31 386397.0 32 470990.0 33 581233.0 34 748804.0 35 1035735.0 36 1360126.0 37 1927791.0 38 1742165.0 39 356739.0 40 612.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.48276554861642 30.005164872754825 29.188792052090378 23.323277526538376 2 22.62716641711583 29.094377136464256 27.443006782331203 20.83544966408871 3 21.715807858761146 28.019414665633903 27.96248251221309 22.302294963391862 4 22.117339242052314 27.85059244079816 28.082876542047728 21.9491917751018 5 22.65326383023134 27.93788358980246 28.274766321033777 21.134086258932427 6 22.592316866340067 28.094453365767407 27.21268574472783 22.100544023164694 7 22.857241056453002 28.183781277807768 27.699206299938123 21.259771365801107 8 22.03935093937269 28.360994767001067 27.663055614528126 21.936598679098115 9 21.630007426204266 28.046732566666886 28.32869082931354 21.994569177815308 10-11 21.768705972434546 28.419615949270167 27.68541509047876 22.12626298781653 12-13 21.747580370360375 28.53952067778738 27.545321880410857 22.16757707144139 14-15 21.942690060568502 28.567447499100084 27.37266859443066 22.11719384590075 16-17 22.077739960312577 28.36716502177749 27.38102800730107 22.174067010608862 18-19 22.033591327002572 28.36668366972337 27.37710963129112 22.222615371982936 20-21 22.165339881359504 28.29186293536146 27.391258321341315 22.15153886193772 22-23 22.10135610649063 28.313473581172214 27.404194015490845 22.180976296846314 24-25 22.0938124490548 28.273663989075825 27.339685109292454 22.292838452576927 26-27 22.302912846691665 28.155879985051087 27.413971094453792 22.12723607380346 28-29 22.278868421066605 28.22410924164763 27.346405604859957 22.15061673242581 30-31 22.239927057943376 28.227983124425766 27.321420873648922 22.210668943981933 32-33 22.287469190213084 28.25716288324575 27.239829270576788 22.21553865596438 34-35 22.27803621133477 28.286942249102786 27.23565462051878 22.199366919043666 36-37 22.289911709099098 28.283591520651974 27.210060244726762 22.216436525522163 38-39 22.28945969782237 28.283001772091893 27.2112216717522 22.21631685833354 40-41 22.288193014337086 28.337298532268147 27.188066672498124 22.186441780896644 42-43 22.34169576242587 28.313299743176596 27.134652980424452 22.210351513973084 44-45 22.302567789581225 28.3466276986535 27.15423796934841 22.19656654241686 46-47 22.316112490089722 28.37485162040487 27.128798533324815 22.180237356180594 48-49 22.333690827588256 28.350547962611017 27.108923234255805 22.20683797554492 50-51 22.37568923415252 28.34904786637867 27.074934114918758 22.200328784550052 52-53 22.296882740063907 28.422568307116276 27.038648199143218 22.241900753676596 54-55 22.34341227572103 28.402759400003998 26.988755650632406 22.265072673642567 56-57 22.349704103879702 28.444205980096914 26.98623302842239 22.21985688760099 58-59 22.32458600113401 28.491160258812105 26.940267750176943 22.243985989876943 60-61 22.328944872220042 28.480716790027273 26.9531451764339 22.23719316131879 62-63 22.31765697967674 28.540727253400718 26.925644835084928 22.21597093183762 64-65 22.33848667869388 28.595276484387593 26.908718316874964 22.157518520043567 66-67 22.30612616398738 28.606857785956418 26.9143052632458 22.172710786810406 68-69 22.295621163629978 28.650170747233418 26.904056240604657 22.15015184853195 70-71 22.298914106001984 28.706045912148543 26.855652610466002 22.13938737138347 72-73 22.286700441942813 28.69151619451277 26.83616738312411 22.185615980420316 74-75 22.341592954272834 28.702344075122323 26.809432682680594 22.146630287924253 76-77 22.279302946540643 28.785778218706167 26.810632188263632 22.12428664648956 78-79 22.300489585625254 28.776529278062256 26.773221285345944 22.14975985096655 80-81 22.24843454566791 28.807424993136905 26.776407325571782 22.167733135623408 82-83 22.257176166382127 28.87575690301779 26.732951239078943 22.134115691521135 84-85 22.265369006376183 28.87605180343088 26.73337823293548 22.12520095725746 86-87 22.251162109032748 28.91005386632543 26.711969295817973 22.126814728823845 88-89 22.220012392638484 28.979295913538078 26.71218937063817 22.08850232318526 90-91 22.205761870713772 28.955532045824796 26.714596974710865 22.12410910875057 92-93 22.202153247531747 29.026574380813774 26.676138995304015 22.09513337635046 94-95 22.163178254459005 29.083588468409467 26.644004119749233 22.1092291573823 96-97 22.178917965339807 29.096185283670184 26.638488353878543 22.086408397111466 98-99 22.155045649507823 29.159578306233197 26.60780716789038 22.0775688763686 100-101 22.24509946087746 29.11923044172288 26.52339624995004 22.112273847449618 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 133709.0 1 75305.5 2 12641.0 3 7911.5 4 6821.5 5 5885.0 6 5537.0 7 5606.0 8 5292.0 9 4802.0 10 4725.5 11 5321.0 12 9026.5 13 9042.5 14 6018.5 15 6394.0 16 6988.0 17 7964.5 18 9424.5 19 11147.0 20 13902.0 21 17997.5 22 23404.5 23 30161.5 24 38338.0 25 48268.5 26 62386.0 27 77448.0 28 88142.5 29 98531.0 30 114667.5 31 135346.0 32 155724.5 33 176602.0 34 201119.5 35 224963.5 36 247782.5 37 270854.0 38 286560.0 39 291626.0 40 301481.0 41 316835.0 42 327522.5 43 343099.5 44 358702.0 45 371795.5 46 383621.5 47 390764.5 48 400782.0 49 410804.0 50 413162.0 51 404469.5 52 390926.5 53 374943.0 54 348907.5 55 322767.5 56 295454.5 57 263309.0 58 237919.5 59 208331.5 60 169278.5 61 130979.0 62 97737.5 63 72027.5 64 52602.0 65 35991.0 66 23071.5 67 15568.5 68 9373.0 69 4615.0 70 2231.0 71 1002.0 72 525.0 73 266.5 74 155.5 75 109.5 76 78.0 77 46.0 78 30.5 79 27.5 80 19.5 81 18.0 82 15.5 83 11.0 84 9.5 85 7.0 86 4.0 87 2.5 88 3.0 89 2.5 90 2.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1447874351113923 2 0.0 3 0.0 4 0.0035528289738609295 5 3.475593561385692E-4 6 0.0010812957746533264 7 0.0 8 1.9308853118809398E-4 9 2.6066951710392695E-4 10-11 1.3998918511136816E-4 12-13 4.344491951732115E-5 14-15 1.448163983910705E-5 16-17 9.6544265594047E-6 18-19 1.448163983910705E-5 20-21 1.2068033199255876E-4 22-23 1.6895246478958225E-4 24-25 0.0 26-27 0.0 28-29 0.005546468058378 30-31 0.0012792115191211228 32-33 3.185960764603551E-4 34-35 2.510150905445222E-4 36-37 2.317062374257128E-4 38-39 0.0012357665996038016 40-41 0.0031763063380441468 42-43 4.585852615717233E-4 44-45 4.441036217326162E-4 46-47 0.006174005784739305 48-49 0.0064395025151229345 50-51 0.006801543511100611 52-53 0.008828973088575599 54-55 0.004576198189157828 56-57 4.82721327970235E-6 58-59 3.86177062376188E-5 60-61 9.461338028216606E-4 62-63 7.868357645914832E-4 64-65 4.199675553341045E-4 66-67 6.420193662004126E-4 68-69 8.54416750507316E-4 70-71 0.0011392223340097548 72-73 0.0025873863179204596 74-75 6.034016599627938E-4 76-77 0.0 78-79 0.0 80-81 7.240819919553526E-5 82-83 2.654967303836293E-4 84-85 1.834341046286893E-4 86-87 0.0025680774648016505 88-89 0.0017474512072522506 90-91 5.503023138860679E-4 92-93 5.165118209281515E-4 94-95 2.992872233415457E-4 96-97 9.219977364231489E-4 98-99 0.0 100-101 9.6544265594047E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0357943E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.71535402048615 #Duplication Level Percentage of deduplicated Percentage of total 1 91.39943497723273 61.891450967557184 2 4.885761144924518 6.616820911761991 3 1.166096307930003 2.368878729403859 4 0.5456677932773868 1.4780035119742279 5 0.32739476261198314 1.1084826127361729 6 0.21892860509308146 0.8894896799453532 7 0.16259166686042692 0.7706966597564328 8 0.1228306237160866 0.665401533559354 9 0.09877212385890123 0.6019550401014723 >10 1.0017102707649634 14.486525641289717 >50 0.0663473181117111 2.885448847676264 >100 0.004094630855818002 0.3805884522922516 >500 5.282496605704386E-5 0.022106238696018455 >1k 5.282496605684452E-5 0.10373477705212233 >5k 1.0564993211368904E-4 0.3884330841410333 >10k+ 1.5847489817053355E-4 5.341983312056534 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 125833 1.2148454572495717 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12166 0.11745575352171758 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 11341 0.1094908516102087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 9.6544265594047E-6 3 1.93088531188094E-5 0.0 0.0 0.0 1.93088531188094E-5 4 1.93088531188094E-5 0.0 0.0 0.0 3.86177062376188E-5 5 1.93088531188094E-5 0.0 0.0 0.0 4.82721327970235E-5 6 1.93088531188094E-5 0.0 0.0 0.0 6.75809859158329E-5 7 3.86177062376188E-5 0.0 0.0 0.0 6.75809859158329E-5 8 3.86177062376188E-5 0.0 0.0 0.0 6.75809859158329E-5 9 7.72354124752376E-5 0.0 0.0 0.0 6.75809859158329E-5 10-11 1.2068033199255876E-4 9.6544265594047E-6 0.0 0.0 8.68898390346423E-5 12-13 1.6412525150987992E-4 9.6544265594047E-6 0.0 0.0 1.3033475855196345E-4 14-15 1.8826131790839167E-4 9.6544265594047E-6 0.0 0.0 1.351619718316658E-4 16-17 2.4136066398511752E-4 9.6544265594047E-6 0.0 0.0 1.6895246478958225E-4 18-19 3.185960764603551E-4 9.6544265594047E-6 0.0 0.0 1.8826131790839167E-4 20-21 4.0065870221529505E-4 9.6544265594047E-6 0.0 0.0 2.220518108663081E-4 22-23 4.151403420544021E-4 9.6544265594047E-6 0.0 0.0 2.5101509054452223E-4 24-25 4.5375804829202095E-4 9.6544265594047E-6 0.0 0.0 2.703239436633316E-4 26-27 5.068573943687467E-4 9.6544265594047E-6 0.0 0.0 2.9928722334154575E-4 28-29 5.503023138860679E-4 9.6544265594047E-6 0.0 4.82721327970235E-6 3.2825050301975983E-4 30-31 6.275377263613056E-4 9.6544265594047E-6 0.0 9.6544265594047E-6 3.3307771629946216E-4 32-33 6.565010060395197E-4 9.6544265594047E-6 0.0 9.6544265594047E-6 4.151403420544021E-4 34-35 6.90291498997436E-4 9.6544265594047E-6 0.0 9.6544265594047E-6 4.4410362173261624E-4 36-37 7.72354124752376E-4 9.6544265594047E-6 0.0 9.6544265594047E-6 4.634124748514256E-4 38-39 8.39935110668209E-4 9.6544265594047E-6 0.0 9.6544265594047E-6 4.730669014108303E-4 40-41 8.930344567449349E-4 9.6544265594047E-6 0.0 9.6544265594047E-6 4.7789411469053265E-4 42-43 9.606154426607677E-4 9.6544265594047E-6 0.0 9.6544265594047E-6 5.020301810890445E-4 44-45 0.001033023641856303 9.6544265594047E-6 0.0 9.6544265594047E-6 5.116846076484491E-4 46-47 0.0011295679074503498 9.6544265594047E-6 0.0 9.6544265594047E-6 5.213390342078538E-4 48-49 0.0012068033199255875 9.6544265594047E-6 0.0 9.6544265594047E-6 5.406478873266632E-4 50-51 0.001298520372239932 9.6544265594047E-6 0.0 9.6544265594047E-6 5.599567404454726E-4 52-53 0.0013998918511136816 9.6544265594047E-6 0.0 9.6544265594047E-6 5.79265593564282E-4 54-55 0.0014867816901483239 9.6544265594047E-6 0.0 9.6544265594047E-6 5.889200201236867E-4 56-57 0.001544708249504752 9.6544265594047E-6 0.0 1.93088531188094E-5 6.034016599627938E-4 58-59 0.001655734154937906 9.6544265594047E-6 0.0 1.93088531188094E-5 6.082288732424962E-4 60-61 0.0017860689134898697 9.6544265594047E-6 0.0 1.93088531188094E-5 6.178832998019008E-4 62-63 0.0019212308853215352 9.6544265594047E-6 0.0 1.93088531188094E-5 6.227105130816031E-4 64-65 0.0020515656438734988 9.6544265594047E-6 0.0 1.93088531188094E-5 6.275377263613055E-4 66-67 0.0021770731891457597 9.6544265594047E-6 0.0 1.93088531188094E-5 6.275377263613055E-4 68-69 0.0023411984406556396 9.6544265594047E-6 0.0 2.413606639851175E-5 6.275377263613055E-4 70-71 0.002558423038242246 9.6544265594047E-6 0.0 2.89632796782141E-5 6.275377263613055E-4 72-73 0.0027563387827100423 9.6544265594047E-6 0.0 2.89632796782141E-5 6.275377263613055E-4 74-75 0.002988045020135755 9.6544265594047E-6 0.0 2.89632796782141E-5 6.323649396410079E-4 76-77 0.0032583689637990863 9.6544265594047E-6 0.0 2.89632796782141E-5 6.371921529207102E-4 78-79 0.003557656187140632 1.448163983910705E-5 0.0 2.89632796782141E-5 6.516737927598173E-4 80-81 0.004011414235432653 1.93088531188094E-5 0.0 2.89632796782141E-5 6.565010060395197E-4 82-83 0.004518271629801399 1.93088531188094E-5 0.0 2.89632796782141E-5 6.613282193192219E-4 84-85 0.005227871981917645 1.93088531188094E-5 0.0 2.89632796782141E-5 6.75809859158329E-4 86-87 0.00622227791753633 1.93088531188094E-5 0.0 2.89632796782141E-5 6.90291498997436E-4 88-89 0.007626996981929713 1.93088531188094E-5 0.0 3.86177062376188E-5 6.951187122771384E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 415 0.0 24.035492 3 CGCGTAG 285 0.0 19.999939 54-55 CAACGCA 7505 0.0 19.334545 12-13 ATCAACG 7450 0.0 19.19043 10-11 CTCGCGT 295 0.0 18.517519 52-53 ACGTACG 325 0.0 18.268469 20-21 AGTGGTA 9395 0.0 17.84669 5 GGTATCA 9650 0.0 17.473366 8 GTATCAA 10055 0.0 16.722328 9 TCAACGC 7365 0.0 16.542042 10-11 TCGCGTA 310 0.0 16.089205 52-53 TGGTATC 10930 0.0 15.383623 7 AAGCAGT 11545 0.0 15.245368 1 AACGCAG 7975 0.0 15.187383 12-13 ACTCGCG 335 0.0 14.888733 50-51 ACGCAGA 10040 0.0 14.547382 14-15 CGTACGC 375 0.0 13.932754 20-21 CGCTAGT 365 0.0 13.663914 90-91 AGAGTAC 10940 0.0 12.742843 18-19 GTGGTAT 13660 0.0 12.344042 6 >>END_MODULE