FastQCFastQC Report
Sat 18 Jun 2016
SRR578561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10452514
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT129742412.412554529943705No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT302860.2897484758212235No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAGCACTCGGCCAGCTTC288050.27557963567425026No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG112130.10727562766239776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2840300.091.7339865
GGTATCA2860100.091.533078
GTATCAA2869700.091.329539
AAGCAGT2868200.091.311781
TGGTATC2858900.091.284367
GCAGTGG2880700.090.691943
GTGGTAT2881350.090.621566
AGCAGTG2892000.090.5002062
CAGTGGT2893350.090.0645754
TCAACGC2854650.046.41745412-13
AACGCAG2864050.046.2924814-15
CAACGCA2854750.046.20112612-13
ATCAACG2856500.046.16975810-11
CGCAGAG2877000.046.09823616-17
ACGCAGA2870400.045.99226814-15
TATCAAC2880800.045.956810-11
CAGAGTA2856450.045.82488618-19
AGAGTAC2804250.045.67339718-19
GAGTACT1627250.045.5856120-21
GCAGAGT2894750.045.3971116-17
Produced by FastQC (version 0.11.5)