FastQCFastQC Report
Sat 18 Jun 2016
SRR578561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10452514
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1311321.2545498623584719No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123210.11787594831253036No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115190.11020315304050299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG73250.019.21767810-11
CAACGCA73200.019.1658112-13
CGTCACG4303.6379788E-1217.6660963
TCAACGC71450.017.47581110-11
GGTATCA95800.016.8995768
AGTGGTA97450.016.7597565
GTATCAA99050.016.4888739
AACGCAG77300.016.1224312-13
AAGCAGT110850.015.2707491
CTCGCGT2201.6789272E-915.10756752-53
TGGTATC112500.014.6864147
TCGCGTA2303.1450327E-914.45071752-53
ACGCAGA100950.014.13249814-15
AGAGTAC109050.012.86520618-19
TATCAAC103050.012.3934238
CGCGTAG2702.9796865E-812.30931154-55
GCAGTGG138300.012.2212853
GTGGTAT136400.012.147876
CAGTGGT153500.011.3514924
CGCTAGT3152.0796506E-811.30446890-91
Produced by FastQC (version 0.11.5)