##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578561_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10452514 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.399766697274934 31.0 31.0 34.0 30.0 34.0 2 31.77941574629797 31.0 31.0 34.0 30.0 34.0 3 31.85460560014557 31.0 31.0 34.0 30.0 34.0 4 35.55703336058674 37.0 35.0 37.0 33.0 37.0 5 35.37857275292815 37.0 35.0 37.0 33.0 37.0 6 35.37117615915176 37.0 35.0 37.0 32.0 37.0 7 35.36324887964752 37.0 35.0 37.0 32.0 37.0 8 35.31831079106902 37.0 35.0 37.0 32.0 37.0 9 36.85948595715825 39.0 37.0 39.0 32.0 39.0 10-11 36.85503090452689 39.0 37.0 39.0 32.0 39.0 12-13 36.844419151220464 39.0 37.0 39.0 32.0 39.0 14-15 38.01695262020218 40.0 37.0 41.0 33.0 41.0 16-17 37.94930879786432 40.0 37.0 41.0 33.0 41.0 18-19 37.88466291458686 40.0 37.0 41.0 32.5 41.0 20-21 37.84170473246915 40.0 37.0 41.0 32.0 41.0 22-23 37.64034446641258 39.5 37.0 41.0 32.0 41.0 24-25 37.64230624326358 39.0 36.0 41.0 32.0 41.0 26-27 37.46838530902709 39.0 36.0 41.0 32.0 41.0 28-29 37.31673581111683 39.0 36.0 41.0 31.5 41.0 30-31 37.17680588612462 39.0 36.0 41.0 31.0 41.0 32-33 37.03560813216801 39.0 36.0 40.5 31.0 41.0 34-35 36.886319549536125 39.0 35.5 40.0 30.0 41.0 36-37 36.731556494447176 39.0 35.0 40.0 30.0 41.0 38-39 36.55758303696125 38.0 35.0 40.0 30.0 41.0 40-41 36.39162903776068 38.0 35.0 40.0 30.0 41.0 42-43 36.475919238185185 38.0 35.0 40.0 30.0 41.0 44-45 36.404098860809945 38.0 35.0 40.0 30.0 41.0 46-47 36.24907424185225 38.0 35.0 40.0 30.0 41.0 48-49 36.094122332675184 38.0 35.0 40.0 29.0 41.0 50-51 35.899679923891995 38.0 34.5 40.0 28.5 41.0 52-53 35.946825902361866 38.0 34.5 40.0 28.0 41.0 54-55 35.930427072377036 38.0 34.5 40.0 28.0 41.0 56-57 35.7614458110269 38.0 34.0 40.0 28.0 41.0 58-59 35.526067508735224 38.0 34.0 40.0 28.0 41.0 60-61 35.25595521804611 38.0 34.0 40.0 28.0 41.0 62-63 34.96281095629243 37.0 34.0 40.0 27.0 41.0 64-65 34.62742432107721 37.0 33.0 39.5 26.5 41.0 66-67 34.27374194380414 36.0 33.0 39.0 26.0 41.0 68-69 33.88560110993394 36.0 32.5 39.0 26.0 40.5 70-71 33.480916887554514 35.0 32.0 38.5 26.0 40.0 72-73 33.07845351845499 35.0 32.0 37.5 25.5 40.0 74-75 32.66017515020788 35.0 31.0 37.0 25.0 39.0 76-77 31.283054488135583 33.5 29.5 36.0 23.0 38.5 78-79 31.93508532971111 34.0 31.0 36.0 24.0 39.0 80-81 31.81454786857975 34.5 31.0 36.0 24.0 37.5 82-83 31.456620483837668 34.0 31.0 35.5 23.0 37.0 84-85 31.178158862068972 34.0 31.0 35.0 23.0 37.0 86-87 30.867738852107735 34.0 31.0 35.0 21.5 36.0 88-89 30.56122249632959 34.0 30.5 35.0 20.0 36.0 90-91 30.221787457065354 34.0 30.0 35.0 19.5 36.0 92-93 29.851288646922644 34.0 30.0 35.0 17.5 35.0 94-95 29.463406219785977 34.0 29.5 35.0 9.5 35.0 96-97 28.99280948104925 34.0 29.0 35.0 2.0 35.0 98-99 28.177493998094622 34.0 29.0 35.0 2.0 35.0 100-101 27.10534805310952 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.39631803651764486 1101 2 0.27386529497394463 1101 3 0.3599976131411182 1101 4 0.3144116486407782 1101 5 0.33847612678476224 1101 6 0.25553402812509063 1101 7 0.27734247855733685 1101 8 0.2179268217762811 1101 9 0.32037730621010496 1101 10-11 0.35318705019032137 1101 12-13 0.3898298874407189 1101 14-15 0.2523994933064273 1101 16-17 0.3721047518598226 1101 18-19 0.4323019048057617 1101 20-21 0.28751199971814145 1101 22-23 0.4490282192382651 1101 24-25 0.37070435088661924 1101 26-27 0.39919867099805373 1101 28-29 0.35226522045135056 1101 30-31 0.34821513687143835 1101 32-33 0.3548252553607725 1101 34-35 0.4253609301936905 1101 36-37 0.39884069630969066 1101 38-39 0.37650598911556443 1101 40-41 0.3656687757742816 1101 42-43 0.3177213324483148 1101 44-45 0.31326494371820246 1101 46-47 0.2659451209357613 1101 48-49 0.24537364948767504 1101 50-51 0.34847536439145443 1101 52-53 0.37985949645084816 1101 54-55 0.45080875166167544 1101 56-57 0.5141034589427989 1101 58-59 0.48722313743958523 1101 60-61 0.36552121806813886 1101 62-63 0.29783459891653763 1101 64-65 0.3644132675417424 1101 66-67 0.32765086782475805 1101 68-69 0.3551487869485186 1101 70-71 0.3326794804967079 1101 72-73 0.4136596573919462 1101 74-75 0.38701484267423325 1101 76-77 0.2926685242113507 1101 78-79 0.36818777311352235 1101 80-81 0.31935169257073426 1101 82-83 0.45941039752171164 1101 84-85 0.4216421302356501 1101 86-87 0.42713597027121963 1101 88-89 0.42014839795130143 1101 90-91 0.3991579921970114 1101 92-93 0.5168709606413877 1101 94-95 0.44540810998877234 1101 96-97 0.4840940919792551 1101 98-99 0.8407192723234829 1101 100-101 1.0728305563072418 1102 1 0.25376243827630063 1102 2 0.27997490054571017 1102 3 0.25434197671329883 1102 4 0.24359557709477997 1102 5 0.2654560840758933 1102 6 0.206664490127622 1102 7 0.2309959591604951 1102 8 0.08376203847588926 1102 9 0.2285338492302671 1102 10-11 0.24057500479879224 1102 12-13 0.30139860175626865 1102 14-15 0.27278198682569155 1102 16-17 0.19606832357598591 1102 18-19 0.27041083586513537 1102 20-21 0.2782975028547767 1102 22-23 0.4350977117042234 1102 24-25 0.3278187549564535 1102 26-27 0.3811230944610884 1102 28-29 0.35976739223511345 1102 30-31 0.41723199275920564 1102 32-33 0.4459779428158086 1102 34-35 0.3140892186050337 1102 36-37 0.3637101470127959 1102 38-39 0.285432356897104 1102 40-41 0.32987811573276105 1102 42-43 0.24541984348335433 1102 44-45 0.32972060414236637 1102 46-47 0.3061658833808778 1102 48-49 0.2530555534431116 1102 50-51 0.322794814460039 1102 52-53 0.3080386071789363 1102 54-55 0.27452955275473556 1102 56-57 0.3116033599194523 1102 58-59 0.3220322069778092 1102 60-61 0.3897330114981372 1102 62-63 0.1843445433596358 1102 64-65 0.14102479070530904 1102 66-67 0.2394001411088027 1102 68-69 0.21500995430104552 1102 70-71 0.2807284995503352 1102 72-73 0.39823000376598117 1102 74-75 0.28753056200478255 1102 76-77 0.10778942690428295 1102 78-79 0.17673421813710277 1102 80-81 0.2848468265829638 1102 82-83 0.3941125795346885 1102 84-85 0.2937458417073948 1102 86-87 0.29486981052714967 1102 88-89 0.2034671758148363 1102 90-91 0.27377598321915286 1102 92-93 0.2671152983329037 1102 94-95 0.3201732804608177 1102 96-97 0.4845831230772042 1102 98-99 0.8328868432751548 1102 100-101 0.9163658607242233 1103 1 0.19675899579000955 1103 2 0.21109262462739764 1103 3 0.25054500283740566 1103 4 0.21781150063009136 1103 5 0.23042996261351334 1103 6 0.07197313147248252 1103 7 0.2293314858298885 1103 8 0.03854826563574676 1103 9 0.19003966245561088 1103 10-11 0.15463532834319693 1103 12-13 0.22262423385752328 1103 14-15 0.31377370102280366 1103 16-17 0.24480420372633915 1103 18-19 0.2079720762682271 1103 20-21 0.35684420368264824 1103 22-23 0.31597911868892226 1103 24-25 0.3267280278101694 1103 26-27 0.1547589601769701 1103 28-29 0.2531163680510815 1103 30-31 0.27789303605779736 1103 32-33 0.16509423142299795 1103 34-35 0.22247771322899723 1103 36-37 0.23857799026425397 1103 38-39 0.323602971342126 1103 40-41 0.24456027126709756 1103 42-43 0.24128915292251207 1103 44-45 0.14589017198750298 1103 46-47 0.28948999602027214 1103 48-49 0.26291026423189834 1103 50-51 0.1768477264254784 1103 52-53 0.12286930840490129 1103 54-55 0.3005076807715241 1103 56-57 0.20321955218052778 1103 58-59 0.18359774193793754 1103 60-61 0.1541137074686958 1103 62-63 0.25764031646347973 1103 64-65 0.12427694684694046 1103 66-67 0.06004600562297924 1103 68-69 0.33775672720206273 1103 70-71 0.24764298086233083 1103 72-73 0.1815947149511814 1103 74-75 0.19676862916608684 1103 76-77 0.11826645646511125 1103 78-79 0.3170347026671543 1103 80-81 0.23667365735850865 1103 82-83 0.4302596775179772 1103 84-85 0.2950005225765402 1103 86-87 0.14301168149932764 1103 88-89 0.2465389512664018 1103 90-91 0.3269422601601093 1103 92-93 0.33225337949516387 1103 94-95 0.4424359210072133 1103 96-97 0.4427815687837864 1103 98-99 1.023890087778895 1103 100-101 0.7705973390601564 1104 1 0.2766500685874931 1104 2 0.24843784636931332 1104 3 0.16789385467922102 1104 4 0.2092887319953789 1104 5 0.1882487743851371 1104 6 0.14361977159428108 1104 7 0.19574987348531891 1104 8 0.11195144708160143 1104 9 0.2788991828279421 1104 10-11 0.16683821552263822 1104 12-13 0.22746297765220191 1104 14-15 0.3059169038034426 1104 16-17 0.3809171532298379 1104 18-19 0.15684035523201345 1104 20-21 0.20538448119092578 1104 22-23 0.34300043096642696 1104 24-25 0.2505448827671941 1104 26-27 0.3527056063429157 1104 28-29 0.2562803046704403 1104 30-31 0.2295837646605392 1104 32-33 0.24893544034742376 1104 34-35 0.19399677419787054 1104 36-37 0.2025293018022225 1104 38-39 0.2658870021648809 1104 40-41 0.33793190592369626 1104 42-43 0.2418943532212623 1104 44-45 0.1952992258280446 1104 46-47 0.22411841512151653 1104 48-49 0.2377235740249759 1104 50-51 0.2824919745036496 1104 52-53 0.21244295826551252 1104 54-55 0.2238148612847084 1104 56-57 0.1990618495207599 1104 58-59 0.24709261212773015 1104 60-61 0.27494938442868033 1104 62-63 0.24998360285057686 1104 64-65 0.27137410068315404 1104 66-67 0.3339042122156215 1104 68-69 0.317412230974341 1104 70-71 0.33519656123441877 1104 72-73 0.3343431761959792 1104 74-75 0.32190770682663583 1104 76-77 0.2365552424369426 1104 78-79 0.3234107520425802 1104 80-81 0.4539041615489623 1104 82-83 0.5461175239908336 1104 84-85 0.4497645102658474 1104 86-87 0.5121712416589652 1104 88-89 0.48678093528652155 1104 90-91 0.535901696611667 1104 92-93 0.5504700584169129 1104 94-95 0.5238190766789224 1104 96-97 0.5202872363892546 1104 98-99 0.8684868585723748 1104 100-101 0.9504609539886424 1105 1 0.2386295717636635 1105 2 0.13208894347103595 1105 3 0.1075615103104468 1105 4 0.15802573437268563 1105 5 0.15074732622791487 1105 6 0.022332557246976137 1105 7 0.1507079894159702 1105 8 0.042465969108604895 1105 9 0.17816092943693462 1105 10-11 0.06661199100560822 1105 12-13 0.04270613494901454 1105 14-15 0.0794367709952013 1105 16-17 0.08081009879236944 1105 18-19 0.1617157745389548 1105 20-21 0.0881450699327857 1105 22-23 0.1840397402105154 1105 24-25 0.19494282132622232 1105 26-27 0.14487155070469981 1105 28-29 0.18468963422095896 1105 30-31 0.15606217458633864 1105 32-33 0.20146789726860703 1105 34-35 0.17243016863749006 1105 36-37 0.15068250875894762 1105 38-39 0.20206121033054814 1105 40-41 0.1738199440098711 1105 42-43 0.21784579164783224 1105 44-45 0.1928422637433158 1105 46-47 0.16903050582514112 1105 48-49 0.16210745982581187 1105 50-51 0.1574230072076972 1105 52-53 0.2095337568738671 1105 54-55 0.19986039093169694 1105 56-57 0.1649076031494019 1105 58-59 0.10750849896187731 1105 60-61 0.019976407337054525 1105 62-63 0.11937868842722565 1105 64-65 0.1621453821118024 1105 66-67 0.20762670528821303 1105 68-69 0.1259826507260442 1105 70-71 0.16086862578073635 1105 72-73 0.21934981258660002 1105 74-75 0.2864428998376525 1105 76-77 0.1463588542918437 1105 78-79 0.30497053679112796 1105 80-81 0.3698930946812311 1105 82-83 0.39798431737660067 1105 84-85 0.2952321820235362 1105 86-87 0.3540376471361917 1105 88-89 0.340596631099114 1105 90-91 0.4796546202253005 1105 92-93 0.6251542301469009 1105 94-95 0.6351581095754391 1105 96-97 0.689732360087703 1105 98-99 1.1125588950256535 1105 100-101 1.2052853454696866 1106 1 0.16614167783596656 1106 2 0.14458969822958068 1106 3 0.12108669852451115 1106 4 0.053327149055583334 1106 5 0.11926371678104886 1106 6 0.05181489946379969 1106 7 0.09926616068462835 1106 8 0.07458199615473404 1106 9 0.12796981310849276 1106 10-11 -0.009648205240956997 1106 12-13 0.0592180863754308 1106 14-15 0.0068445908797087895 1106 16-17 0.007650454897429881 1106 18-19 0.08381329588217312 1106 20-21 0.10559062901842964 1106 22-23 0.22186229811121194 1106 24-25 0.12445826676454885 1106 26-27 0.06007877831499542 1106 28-29 0.1401547527416227 1106 30-31 0.1408084937637284 1106 32-33 0.10907325625384345 1106 34-35 0.12392986006757667 1106 36-37 0.018015794591683232 1106 38-39 0.05042180683054909 1106 40-41 0.16591020183708594 1106 42-43 0.15746058581552091 1106 44-45 0.09558676113951492 1106 46-47 0.08640498385281603 1106 48-49 0.09899196007035727 1106 50-51 0.10801709029134798 1106 52-53 0.1204815391321219 1106 54-55 0.17185844550174778 1106 56-57 0.17119057845471275 1106 58-59 0.10365611368283112 1106 60-61 0.22445083255073683 1106 62-63 0.2111929827515553 1106 64-65 0.19561174968885808 1106 66-67 0.14581808640844685 1106 68-69 0.321675100397556 1106 70-71 0.25463403569975895 1106 72-73 0.2927940439765848 1106 74-75 0.385980189325835 1106 76-77 0.18497059027556162 1106 78-79 0.30738363255463597 1106 80-81 0.3589493420480636 1106 82-83 0.5956687521280593 1106 84-85 0.5798556422205614 1106 86-87 0.5901316359971034 1106 88-89 0.6075369202079415 1106 90-91 0.5889802299831572 1106 92-93 0.6463872886306419 1106 94-95 0.6912610892984823 1106 96-97 0.6928165349219704 1106 98-99 1.1499008277799128 1106 100-101 1.1949776918740902 1107 1 0.2032358155144145 1107 2 0.07869530325979213 1107 3 -0.015879690382920586 1107 4 0.03477359039602845 1107 5 0.019514213692289673 1107 6 0.013176091472033136 1107 7 0.007340935599131626 1107 8 0.04264336497883647 1107 9 0.05811932689233856 1107 10-11 0.006350691066522529 1107 12-13 0.033966783524959965 1107 14-15 0.07138438604337694 1107 16-17 0.0951235193931268 1107 18-19 0.030092619272288346 1107 20-21 0.09656990567675905 1107 22-23 0.16135527476334488 1107 24-25 0.13728541357300372 1107 26-27 0.13058224876122182 1107 28-29 0.060921627818672164 1107 30-31 0.047764713875757536 1107 32-33 0.041621651317896635 1107 34-35 0.13392165767926656 1107 36-37 0.08783468206725331 1107 38-39 0.17379293596368228 1107 40-41 0.1129092691475222 1107 42-43 0.09676301812741173 1107 44-45 0.16847012146863705 1107 46-47 0.05122845693220057 1107 48-49 0.0736101416023871 1107 50-51 0.1795597400452209 1107 52-53 0.11920583675714624 1107 54-55 0.11883915178203353 1107 56-57 0.18172821480123247 1107 58-59 0.13126212980551344 1107 60-61 0.13491864621488503 1107 62-63 0.2000050359470933 1107 64-65 0.2639448067606054 1107 66-67 0.17955320900302496 1107 68-69 0.26857985378057947 1107 70-71 0.24531781823104382 1107 72-73 0.21096472438982516 1107 74-75 0.2681845477605549 1107 76-77 0.15934702388491218 1107 78-79 0.19056624044536719 1107 80-81 0.263560520158606 1107 82-83 0.4298612378299289 1107 84-85 0.38277029076040847 1107 86-87 0.3973764246586846 1107 88-89 0.36302451597826746 1107 90-91 0.508570751088655 1107 92-93 0.5408639607726222 1107 94-95 0.504915011294905 1107 96-97 0.5668294878249149 1107 98-99 1.0540308834409124 1107 100-101 1.1642721350225465 1108 1 0.11180943130811372 1108 2 0.0015862245470614766 1108 3 -0.04058444703781916 1108 4 -0.022515636370762593 1108 5 0.034700424443592226 1108 6 0.07709513889992792 1108 7 -0.015383496009931719 1108 8 0.06029119450201392 1108 9 -0.0104030474139023 1108 10-11 0.04444470515780097 1108 12-13 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>>Per sequence quality scores pass #Quality Count 2 149.0 3 99.0 4 247.0 5 671.0 6 1262.0 7 2370.0 8 4128.0 9 6673.0 10 9389.0 11 12166.0 12 15135.0 13 18303.0 14 21611.0 15 25108.0 16 28806.0 17 33419.0 18 39410.0 19 45129.0 20 48772.0 21 51505.0 22 57301.0 23 68391.0 24 84608.0 25 107201.0 26 135612.0 27 171206.0 28 215399.0 29 266239.0 30 325351.0 31 394376.0 32 482923.0 33 599143.0 34 769536.0 35 1062975.0 36 1383375.0 37 1922173.0 38 1700183.0 39 341592.0 40 578.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.500566552557633 30.059022079978536 29.16446793168789 23.275943435775936 2 22.591005379184377 29.123098998001822 27.44802829252369 20.83786733029011 3 21.70761024572653 28.09135677790051 27.934848975088673 22.266184001284284 4 22.102180920573332 27.90539148777111 28.03936045055973 21.95306714109583 5 22.623845079825276 27.99038388497094 28.27295867554165 21.112812359662144 6 22.566334344382934 28.156602475349445 27.186633939580414 22.090429240687207 7 22.843246788756826 28.260939446604798 27.630257429470902 21.265556335167474 8 22.019420399723934 28.39598205752224 27.654858917194463 21.929738625559363 9 21.617542679036404 28.06745603466808 28.33157474237974 21.983426543915773 10-11 21.75631710963306 28.458818313652845 27.652531656105545 22.13233292060855 12-13 21.752049315600054 28.57979907991513 27.511156646142737 22.15699495834208 14-15 21.898057252063953 28.595785664577917 27.377131472868633 22.129025610489496 16-17 22.064004031183504 28.402240284119223 27.367670943452975 22.166084741244298 18-19 22.022273495378574 28.426101933573296 27.329839245356595 22.221785325691535 20-21 22.14048430267439 28.334525869520565 27.387016882925664 22.13797294487938 22-23 22.226640115299357 28.332418156577454 27.3200328707828 22.12090885734039 24-25 22.08904958175612 28.310041010229693 27.307617095753233 22.293292312260952 26-27 22.271956187338276 28.20280806411593 27.409988430044535 22.115247318501257 28-29 22.272819251863883 28.26803546698112 27.329098089117128 22.130047192037868 30-31 22.230444902044283 28.273725495523827 27.295703905456126 22.20012569697576 32-33 22.277821900668055 28.30090336774504 27.23104432877938 22.190230402807522 34-35 22.262449958428345 28.339646615341145 27.20021350050077 22.197689925729744 36-37 22.24285797519685 28.338296916238786 27.191136672763154 22.22770843580121 38-39 22.28126874420259 28.33607999159236 27.18587987245224 22.19677139175281 40-41 22.278116823501094 28.38679984676928 27.161331974514102 22.173751355215526 42-43 22.331790574576825 28.35893970363399 27.127521537081577 22.181748184707605 44-45 22.28842577759094 28.416127215427057 27.128165157179048 22.16728184980296 46-47 22.303995062980487 28.424540622772483 27.07416532317864 22.197298991068397 48-49 22.33482409070936 28.398758183239014 27.067400300766696 22.199017425284925 50-51 22.377801388955994 28.411766084769656 27.044653351811927 22.165779174462422 52-53 22.287644181767867 28.478040088626557 27.015645518285066 22.218670211320514 54-55 22.33525208985766 28.47208375733885 26.947386065778613 22.245278087024875 56-57 22.34929856448582 28.49028674885113 26.94120080494262 22.219213881720428 58-59 22.30891966923315 28.558760913485955 26.92587404157571 22.20644537570519 60-61 22.308374808204036 28.55981345731754 26.901609507530917 22.230202226947508 62-63 22.307763140932042 28.57516656896279 26.91632936705827 22.200740923046897 64-65 22.29800746545105 28.65692008431352 26.882459519406805 22.162612930828622 66-67 22.275879791354956 28.652051257544564 26.895520480334024 22.176548470766456 68-69 22.270233242092836 28.698368673479248 26.890697009154984 22.140701075272933 70-71 22.28368659909529 28.766796952117407 26.817236637409803 22.1322798113775 72-73 22.276257034125074 28.75557888782752 26.79263510265689 22.175528975390513 74-75 22.351474680150034 28.75945242013384 26.777507517854833 22.111565381861293 76-77 22.276566708158118 28.836204209893975 26.77009058294861 22.117138498999292 78-79 22.273274561832775 28.821852895211553 26.76503215307187 22.1398403898838 80-81 22.256942834978613 28.86335779785179 26.75802580716575 22.121673560003842 82-83 22.289748214060456 28.926358256193403 26.68379787111347 22.10009565863267 84-85 22.2489669945305 28.942297113324933 26.714143301465388 22.094592590679177 86-87 22.22065212706462 28.98672048701992 26.680032779377292 22.112594606538167 88-89 22.207352288854906 29.06017147211814 26.68124948068577 22.051226758341187 90-91 22.174301783194274 29.01516968193269 26.704856958852822 22.10567157602021 92-93 22.207742768881168 29.095388897377138 26.64195709313084 22.054911240610853 94-95 22.172869473838492 29.131465083007583 26.625312918025283 22.070352525128637 96-97 22.151677023856863 29.134537085524038 26.63273379162997 22.081052098989126 98-99 22.183341954484646 29.172345911206005 26.55288317763728 22.091428956672065 100-101 22.23942811712807 29.14450049701076 26.50490457297735 22.11116681288382 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 138590.0 1 78853.5 2 14038.5 3 8332.0 4 7188.5 5 6233.0 6 5631.5 7 5663.5 8 5535.0 9 4962.5 10 4798.0 11 5589.5 12 9409.0 13 9364.0 14 6156.0 15 6465.5 16 7125.5 17 8117.0 18 9636.5 19 11407.5 20 14193.0 21 18189.5 22 23279.0 23 30219.5 24 38845.5 25 48881.0 26 63078.0 27 78316.5 28 89142.5 29 99449.5 30 115719.5 31 136555.5 32 157057.0 33 177785.0 34 201938.5 35 226333.5 36 249752.5 37 272668.0 38 288888.5 39 294315.0 40 303625.5 41 319122.5 42 330560.0 43 346298.0 44 361903.0 45 374841.5 46 385805.5 47 393543.5 48 404051.5 49 413496.0 50 415762.5 51 407332.0 52 393731.0 53 377627.0 54 351755.5 55 325887.0 56 298598.0 57 265641.5 58 239526.0 59 210727.0 60 171627.5 61 132266.5 62 99207.0 63 73367.0 64 53549.5 65 36555.0 66 23378.0 67 15765.0 68 9420.5 69 4563.5 70 2216.0 71 1026.0 72 491.0 73 260.0 74 166.0 75 104.5 76 70.5 77 59.0 78 49.0 79 31.0 80 23.0 81 16.0 82 12.0 83 12.5 84 10.5 85 5.0 86 3.5 87 3.5 88 3.5 89 2.5 90 0.5 91 2.0 92 2.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15870823038361873 2 0.0 3 0.0 4 8.89738105110407E-4 5 5.74024583942198E-4 6 9.567076399036634E-5 7 1.9134152798073268E-4 8 0.0 9 1.6264029878362276E-4 10-11 2.6787813917302574E-4 12-13 0.0 14-15 0.0 16-17 4.783538199518317E-6 18-19 9.567076399036633E-6 20-21 9.567076399036633E-6 22-23 6.744788861320827E-4 24-25 0.0 26-27 1.0045430218988466E-4 28-29 0.005156654179080746 30-31 0.003759861024821397 32-33 1.4828968418506783E-4 34-35 7.605825737234124E-4 36-37 4.8792089635086834E-4 38-39 0.0011049973240887313 40-41 0.0028892570725090635 42-43 0.0012867717756704271 44-45 6.79262424331601E-4 46-47 0.00826595400876765 48-49 0.00852904860974116 50-51 0.007452752514849538 52-53 0.014063602306583852 54-55 0.0057976482978162 56-57 8.801710287113703E-4 58-59 0.0013059059284685004 60-61 0.0 62-63 1.1002137858892129E-4 64-65 9.375734871055902E-4 66-67 0.0011576162442834327 68-69 0.002717049697326404 70-71 0.001994735429199138 72-73 0.005835916603412346 74-75 0.0022052111099779437 76-77 0.0015355157620453796 78-79 0.0016168359114371912 80-81 2.8222875377158067E-4 82-83 0.0023200160267663836 84-85 3.826830559614653E-5 86-87 0.0020138695819972114 88-89 2.630946009735074E-4 90-91 0.0011432656296848777 92-93 0.0014828968418506782 94-95 9.184393343075168E-4 96-97 0.001085863171290658 98-99 0.5227211367523641 100-101 2.296098335768792E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0452514E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.67013326266465 #Duplication Level Percentage of deduplicated Percentage of total 1 91.42128620215264 61.86490620343874 2 4.909455179685274 6.6444697251276335 3 1.1187822960038836 2.271244411874782 4 0.5506528285514044 1.4905100115814707 5 0.311661094722288 1.0545073906322588 6 0.2259293757069436 0.9173202577223702 7 0.1685097167253249 0.7982152490799622 8 0.12135673320451525 0.6569781046616949 9 0.10340146659636751 0.6297471921718041 >10 0.9994557872485279 14.422051938735056 >50 0.06445802779841223 2.7946664563888337 >100 0.004633153330882376 0.4243091624960546 >500 5.226728418971316E-5 0.022813224857250684 >1k 1.045345683792587E-4 0.13924909498116575 >5k 1.045345683792587E-4 0.38775408389091187 >10k+ 1.5680185256888802E-4 5.481257492359974 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 131132 1.2545498623584719 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12321 0.11787594831253036 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 11519 0.11020315304050299 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.826830559614653E-5 0.0 0.0 0.0 0.0 2 3.826830559614653E-5 0.0 0.0 0.0 9.567076399036633E-6 3 7.653661119229307E-5 0.0 0.0 0.0 2.87012291971099E-5 4 7.653661119229307E-5 0.0 0.0 0.0 2.87012291971099E-5 5 9.567076399036634E-5 0.0 0.0 0.0 2.87012291971099E-5 6 9.567076399036634E-5 0.0 0.0 0.0 2.87012291971099E-5 7 1.2437199318747624E-4 0.0 0.0 0.0 3.826830559614653E-5 8 1.2437199318747624E-4 0.0 0.0 0.0 4.783538199518317E-5 9 1.6264029878362276E-4 0.0 0.0 0.0 4.783538199518317E-5 10-11 1.8655798978121436E-4 0.0 0.0 0.0 7.653661119229307E-5 12-13 2.0090860437976932E-4 0.0 0.0 0.0 7.653661119229307E-5 14-15 2.487439863749525E-4 0.0 0.0 0.0 1.0045430218988466E-4 16-17 3.01362906569654E-4 0.0 0.0 0.0 1.1002137858892129E-4 18-19 4.0181720875953863E-4 0.0 0.0 0.0 1.148049167884396E-4 20-21 4.209513615576119E-4 0.0 0.0 0.0 1.2915553138699457E-4 22-23 4.257348997571302E-4 0.0 0.0 0.0 1.5785676058410443E-4 24-25 4.8313735815135E-4 0.0 0.0 0.0 1.6264029878362276E-4 26-27 5.59673969343643E-4 0.0 0.0 0.0 2.0090860437976932E-4 28-29 6.266435041368995E-4 0.0 0.0 0.0 2.2004275717784258E-4 30-31 6.50561195134491E-4 0.0 0.0 0.0 2.3917690997591584E-4 32-33 7.270978063267842E-4 0.0 0.0 0.0 2.9179583017061733E-4 34-35 7.510154973243758E-4 0.0 0.0 0.0 2.9657936837013566E-4 36-37 8.275521085166688E-4 0.0 0.0 0.0 2.9657936837013566E-4 38-39 8.993051815094435E-4 0.0 0.0 0.0 2.9657936837013566E-4 40-41 9.471405635046267E-4 0.0 0.0 0.0 3.061464447691723E-4 42-43 0.0010188936364974015 0.0 0.0 0.0 3.3006413576676386E-4 44-45 0.0010810796330911396 0.0 0.0 0.0 3.444147503653188E-4 46-47 0.001162399782482951 0.0 0.0 0.0 3.635489031633921E-4 48-49 0.0012102351644781343 0.0 0.0 0.0 3.683324413629104E-4 50-51 0.0013202565430670554 0.0 0.0 0.0 3.731159795624287E-4 52-53 0.0014063602306583851 0.0 0.0 0.0 3.731159795624287E-4 54-55 0.0015020309946487515 0.0 0.0 9.567076399036633E-6 3.731159795624287E-4 56-57 0.0015977017586391177 0.0 0.0 1.435061459855495E-5 3.731159795624287E-4 58-59 0.001688588984429966 0.0 0.0 1.9134152798073266E-5 3.92250132360502E-4 60-61 0.0017794762102208137 0.0 0.0 1.9134152798073266E-5 4.209513615576119E-4 62-63 0.0019229823562063632 0.0 0.0 1.9134152798073266E-5 4.400855143556851E-4 64-65 0.0020760555785909497 0.0 0.0 1.9134152798073266E-5 4.592196671537584E-4 66-67 0.0022291288009755354 0.0 0.0 1.9134152798073266E-5 4.68786743552795E-4 68-69 0.0024061197143577136 0.0 0.0 2.87012291971099E-5 4.7357028175231336E-4 70-71 0.002568760013141336 0.0 0.0 2.87012291971099E-5 4.8313735815135E-4 72-73 0.0028318546141148436 0.0 0.0 2.87012291971099E-5 4.97487972749905E-4 74-75 0.0030853821386893146 0.0 0.0 2.87012291971099E-5 5.070550491489416E-4 76-77 0.00332455904866523 0.0 0.0 2.87012291971099E-5 5.261892019470148E-4 78-79 0.0036498396462324757 0.0 0.0 2.87012291971099E-5 5.357562783460515E-4 80-81 0.004104275775186716 0.0 0.0 2.87012291971099E-5 5.357562783460515E-4 82-83 0.004663949744530359 0.0 0.0 2.87012291971099E-5 5.548904311441247E-4 84-85 0.005338428630662442 0.0 0.0 2.87012291971099E-5 5.835916603412347E-4 86-87 0.006266435041368995 0.0 0.0 3.348476739662822E-5 5.88375198540753E-4 88-89 0.007629743428231715 0.0 0.0 4.3051843795664855E-5 5.931587367402713E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCAACG 7325 0.0 19.217678 10-11 CAACGCA 7320 0.0 19.16581 12-13 CGTCACG 430 3.6379788E-12 17.666096 3 TCAACGC 7145 0.0 17.475811 10-11 GGTATCA 9580 0.0 16.899576 8 AGTGGTA 9745 0.0 16.759756 5 GTATCAA 9905 0.0 16.488873 9 AACGCAG 7730 0.0 16.12243 12-13 AAGCAGT 11085 0.0 15.270749 1 CTCGCGT 220 1.6789272E-9 15.107567 52-53 TGGTATC 11250 0.0 14.686414 7 TCGCGTA 230 3.1450327E-9 14.450717 52-53 ACGCAGA 10095 0.0 14.132498 14-15 AGAGTAC 10905 0.0 12.865206 18-19 TATCAAC 10305 0.0 12.393423 8 CGCGTAG 270 2.9796865E-8 12.309311 54-55 GCAGTGG 13830 0.0 12.221285 3 GTGGTAT 13640 0.0 12.14787 6 CAGTGGT 15350 0.0 11.351492 4 CGCTAGT 315 2.0796506E-8 11.304468 90-91 >>END_MODULE