FastQCFastQC Report
Sat 18 Jun 2016
SRR578560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9166757
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110255412.027743290238849No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT254690.2778408983678743No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC195500.21327062558765328No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG104830.11435887304528744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2486150.091.8434145
GGTATCA2497550.091.577858
GTATCAA2503150.091.427979
TGGTATC2501400.091.364797
AAGCAGT2505300.091.33561
GTGGTAT2521400.090.7142946
GCAGTGG2522650.090.578563
AGCAGTG2533300.090.334772
CAGTGGT2531000.090.183524
TCAACGC2492000.046.404212-13
ATCAACG2490900.046.2833510-11
AACGCAG2499200.046.27641714-15
CAACGCA2492300.046.26053612-13
CGCAGAG2508450.046.08642616-17
ACGCAGA2505400.046.0567614-15
TATCAAC2510750.045.99495310-11
CAGAGTA2496500.045.7762818-19
AGAGTAC2451050.045.7189618-19
GAGTACT1377100.045.47144720-21
GCAGAGT2531050.045.43491416-17
Produced by FastQC (version 0.11.5)