##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578560_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9166757
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.984563461210982	31.0	31.0	34.0	27.0	34.0
2	31.33532916821074	33.0	31.0	34.0	28.0	34.0
3	31.46368579422363	34.0	31.0	34.0	28.0	34.0
4	34.84282074893007	37.0	35.0	37.0	32.0	37.0
5	34.8676333407769	37.0	35.0	37.0	32.0	37.0
6	34.90937645669019	37.0	35.0	37.0	32.0	37.0
7	34.841952066581456	37.0	35.0	37.0	32.0	37.0
8	34.88266264721537	37.0	35.0	37.0	32.0	37.0
9	36.38332138617834	39.0	37.0	39.0	32.0	39.0
10-11	36.266602081848575	39.0	37.0	39.0	32.0	39.0
12-13	36.321710338781756	39.0	37.0	39.0	32.0	39.0
14-15	37.57933361820325	40.0	38.0	41.0	32.0	41.0
16-17	37.55380223343981	40.0	38.0	41.0	32.0	41.0
18-19	37.47771703777028	40.0	38.0	41.0	32.0	41.0
20-21	37.405785655712265	40.0	38.0	41.0	31.0	41.0
22-23	37.1429223006566	39.5	37.5	41.0	31.5	41.0
24-25	37.098974370107115	40.0	37.0	41.0	31.0	41.0
26-27	36.98310793010003	40.0	37.0	41.0	30.0	41.0
28-29	36.82544017475318	40.0	37.0	41.0	30.0	41.0
30-31	36.59373985805449	40.0	36.0	41.0	30.0	41.0
32-33	36.298072044453676	39.5	36.0	41.0	29.0	41.0
34-35	36.12825686335964	39.0	36.0	41.0	27.5	41.0
36-37	35.84297303833843	39.0	35.0	40.5	27.0	41.0
38-39	34.99041029450219	38.0	35.0	40.0	26.5	41.0
40-41	34.764149633289065	38.0	34.0	40.0	25.5	41.0
42-43	34.47043256410092	37.5	34.0	40.0	24.0	41.0
44-45	34.19142085908899	37.0	33.0	40.0	24.0	41.0
46-47	34.04968180131752	37.0	33.0	40.0	23.0	41.0
48-49	34.072832464087355	37.0	33.0	40.0	23.0	41.0
50-51	33.20241727799701	37.0	32.5	39.5	20.0	40.5
52-53	32.79290320448115	37.5	33.0	39.5	11.0	40.5
54-55	32.398070822647526	38.0	32.5	40.0	2.0	41.0
56-57	31.8802910342229	37.5	31.0	40.0	2.0	41.0
58-59	31.575224422333875	37.0	31.0	40.0	2.0	41.0
60-61	31.30460112556709	37.0	31.0	40.0	2.0	41.0
62-63	30.959916522277183	36.0	30.5	40.0	2.0	41.0
64-65	30.65596704483385	36.0	30.0	39.0	2.0	41.0
66-67	30.31597123170168	35.0	29.5	39.0	2.0	41.0
68-69	29.974926083455685	35.0	29.0	39.0	2.0	41.0
70-71	29.672460009575907	35.0	29.0	38.0	2.0	40.0
72-73	29.343194817971067	35.0	29.0	37.0	2.0	39.5
74-75	28.98149618234671	35.0	29.0	37.0	2.0	39.0
76-77	28.60601393709902	35.0	28.0	36.0	2.0	39.0
78-79	28.26319313362403	35.0	27.5	36.0	2.0	38.5
80-81	27.947554244101813	34.5	27.0	35.0	2.0	37.0
82-83	27.683970241602346	34.0	26.0	35.0	2.0	37.0
84-85	27.418008462534786	34.0	26.0	35.0	2.0	36.5
86-87	27.183775625338384	34.0	26.0	35.0	2.0	36.0
88-89	26.93281091666333	34.0	25.0	35.0	2.0	36.0
90-91	26.701835338277213	34.0	25.0	35.0	2.0	35.5
92-93	26.422230293657833	34.0	24.5	35.0	2.0	35.0
94-95	26.138305018885085	34.0	23.5	35.0	2.0	35.0
96-97	25.864879258826214	34.0	20.0	35.0	2.0	35.0
98-99	25.546084618584302	34.0	18.5	35.0	2.0	35.0
100-101	24.690952863700872	33.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5602744349671909
1101	2	0.6007175900498751
1101	3	0.48979840525126406
1101	4	0.5434100631268635
1101	5	0.509644423064131
1101	6	0.46405408395795433
1101	7	0.4697995266077939
1101	8	0.45935339312354984
1101	9	0.5795799126997139
1101	10-11	0.6424700042996179
1101	12-13	0.6727984194055807
1101	14-15	0.809714250291016
1101	16-17	0.7744714830935422
1101	18-19	0.7575688002843322
1101	20-21	0.8389304258735351
1101	22-23	0.8442089699743605
1101	24-25	0.7817120828372879
1101	26-27	0.8538218078761091
1101	28-29	0.932602638528742
1101	30-31	0.9872685746500736
1101	32-33	1.0184197324312478
1101	34-35	1.1002688937906342
1101	36-37	1.0593915783283805
1101	38-39	1.035682785183461
1101	40-41	1.0346239161700979
1101	42-43	1.0650485239879472
1101	44-45	1.177693045169697
1101	46-47	1.1470823216340449
1101	48-49	1.0648925515971825
1101	50-51	1.1207581669466364
1101	52-53	0.9349019339558495
1101	54-55	0.8927244216047754
1101	56-57	0.913923697044094
1101	58-59	0.8995869623998836
1101	60-61	0.8137216663984255
1101	62-63	0.8468708922897861
1101	64-65	0.8533556227929004
1101	66-67	0.8883738304803295
1101	68-69	0.8461533205534799
1101	70-71	0.9486398189516372
1101	72-73	0.9104517500720881
1101	74-75	0.9387463754616796
1101	76-77	0.8832353083920701
1101	78-79	0.8754956064567629
1101	80-81	0.8651288886065736
1101	82-83	0.9192967816053113
1101	84-85	0.9103588093963175
1101	86-87	0.9090851890000096
1101	88-89	0.9038778554255522
1101	90-91	0.9632751159825226
1101	92-93	1.0780182745942959
1101	94-95	1.135095812686906
1101	96-97	1.1312976406501178
1101	98-99	1.0330751330508754
1101	100-101	1.164914873369188
1102	1	0.5475923711524686
1102	2	0.46740947967593627
1102	3	0.5020337420863008
1102	4	0.46294559761481224
1102	5	0.5499599566664486
1102	6	0.5853537308809393
1102	7	0.5633094241329815
1102	8	0.48954756974261926
1102	9	0.636034982857332
1102	10-11	0.6222084035294628
1102	12-13	0.6245094633983683
1102	14-15	0.792654348977841
1102	16-17	0.7746613022296103
1102	18-19	0.8758922741461035
1102	20-21	0.8786627328539254
1102	22-23	0.8845818254120701
1102	24-25	0.9146253035554537
1102	26-27	0.9626500184343101
1102	28-29	1.0288503597769747
1102	30-31	1.0146385851133246
1102	32-33	1.1057847610655216
1102	34-35	1.0490033012423652
1102	36-37	1.0577296892698982
1102	38-39	1.1019673696873937
1102	40-41	1.1470316877846969
1102	42-43	1.2258233431356942
1102	44-45	1.2770142171395165
1102	46-47	1.3022950929679453
1102	48-49	1.2423000884785438
1102	50-51	1.244374822033059
1102	52-53	1.0876315830531311
1102	54-55	1.0287463561360397
1102	56-57	0.9974127209820018
1102	58-59	1.0620889198780397
1102	60-61	1.0739595451114248
1102	62-63	1.1016778928129582
1102	64-65	1.0938296201541924
1102	66-67	1.0629846527308509
1102	68-69	0.9977252983843883
1102	70-71	0.997721035818742
1102	72-73	1.0273377991298425
1102	74-75	0.9540790177465617
1102	76-77	1.0298881911681512
1102	78-79	1.055881585519412
1102	80-81	1.0589526112315006
1102	82-83	1.1042594117082132
1102	84-85	1.1140798109832168
1102	86-87	1.0900481521904233
1102	88-89	1.1262627365430689
1102	90-91	1.0624481723464818
1102	92-93	1.0353274260984584
1102	94-95	1.1436939769552446
1102	96-97	1.2149471444843272
1102	98-99	1.3238550734577963
1102	100-101	1.382365731518533
1103	1	0.3839334214785204
1103	2	0.44290810389300006
1103	3	0.3741232727596042
1103	4	0.4590615361410073
1103	5	0.4833312473097777
1103	6	0.4708449111058144
1103	7	0.37718005922963016
1103	8	0.4060537206453745
1103	9	0.5141513823410335
1103	10-11	0.503457074428205
1103	12-13	0.5514294119110588
1103	14-15	0.6065956749510022
1103	16-17	0.7012349458854814
1103	18-19	0.6741911286431943
1103	20-21	0.6858633209919276
1103	22-23	0.7855375086517782
1103	24-25	0.6890015042810731
1103	26-27	0.7530248057840296
1103	28-29	0.7535306251160208
1103	30-31	0.8757027632340169
1103	32-33	0.8954179377165161
1103	34-35	0.8865709187139359
1103	36-37	0.9449015237913514
1103	38-39	0.8053641530506255
1103	40-41	0.93046517835554
1103	42-43	0.8146050653708059
1103	44-45	0.8080863303978418
1103	46-47	0.9930917291484178
1103	48-49	1.0312244021922936
1103	50-51	0.9546182454827559
1103	52-53	0.911970519655732
1103	54-55	0.911285769572423
1103	56-57	0.9688644802077384
1103	58-59	0.9455802611998827
1103	60-61	0.9578198290890789
1103	62-63	0.8397377140445315
1103	64-65	0.7876598885837893
1103	66-67	0.7843702185150256
1103	68-69	0.9371183870847055
1103	70-71	0.8492491913466189
1103	72-73	0.9212720392968485
1103	74-75	0.9441589774556043
1103	76-77	0.9175318880789156
1103	78-79	0.9035347122965867
1103	80-81	0.9794363273546658
1103	82-83	0.9928266513258492
1103	84-85	0.9924802609822194
1103	86-87	0.9592818629313378
1103	88-89	0.943091341623596
1103	90-91	0.914531161353576
1103	92-93	0.9531827790956875
1103	94-95	0.8639357384911364
1103	96-97	0.8991438425895062
1103	98-99	1.00543579432399
1103	100-101	1.0927163300338343
1104	1	0.36704539556665594
1104	2	0.37745964310003544
1104	3	0.4087570956801123
1104	4	0.38997579141901184
1104	5	0.45381855447858044
1104	6	0.4430062846230385
1104	7	0.42349713685677415
1104	8	0.3033869580106625
1104	9	0.49682285635720547
1104	10-11	0.49690503074546655
1104	12-13	0.5230572220903156
1104	14-15	0.6249362823374227
1104	16-17	0.5838793745007109
1104	18-19	0.6314328430725027
1104	20-21	0.637707886891711
1104	22-23	0.6022332338421919
1104	24-25	0.5768915855085908
1104	26-27	0.6312786334827152
1104	28-29	0.668433105249612
1104	30-31	0.7835276505366764
1104	32-33	0.7872674163100513
1104	34-35	0.8387717077661492
1104	36-37	0.8201230431364976
1104	38-39	0.8599699175741549
1104	40-41	0.8218179104711325
1104	42-43	0.9934133950294992
1104	44-45	0.9168643164434371
1104	46-47	1.1346331310105313
1104	48-49	0.9717461987202398
1104	50-51	0.9264518522729261
1104	52-53	0.806197102540942
1104	54-55	0.8003407135155314
1104	56-57	0.8044014675397442
1104	58-59	0.7744585261932109
1104	60-61	0.8143419700847012
1104	62-63	0.7301838667937304
1104	64-65	0.672497240670971
1104	66-67	0.7419643397429532
1104	68-69	0.7133926869247311
1104	70-71	0.6227111586308922
1104	72-73	0.7014700640893885
1104	74-75	0.7918095773493441
1104	76-77	0.7829065450935033
1104	78-79	0.6962696355311913
1104	80-81	0.8153462326634404
1104	82-83	0.810933849839337
1104	84-85	0.7859391148204047
1104	86-87	0.8034087579363813
1104	88-89	0.815764324103025
1104	90-91	0.7645830521217185
1104	92-93	0.6496291485390167
1104	94-95	0.5460925087901174
1104	96-97	0.6593419703362713
1104	98-99	0.8874789825185196
1104	100-101	0.8358755778833533
1105	1	0.2662208001945494
1105	2	0.24518093163633736
1105	3	0.2523166058712967
1105	4	0.26133198853574413
1105	5	0.2546267934017692
1105	6	0.23626835413639924
1105	7	0.1991255531780638
1105	8	0.22177188788860747
1105	9	0.1832460836375276
1105	10-11	0.22380429457184192
1105	12-13	0.21291341778326256
1105	14-15	0.0776396201648808
1105	16-17	0.12075283501522449
1105	18-19	0.04043243152742804
1105	20-21	0.10529585893284832
1105	22-23	0.12310533165045001
1105	24-25	0.05172151422206639
1105	26-27	-0.24247466308424492
1105	28-29	-0.025848908243695234
1105	30-31	-0.15712651841216285
1105	32-33	-0.11755493418882423
1105	34-35	0.02419975793471707
1105	36-37	0.06816534202932445
1105	38-39	0.07470955393016965
1105	40-41	0.08523696804683567
1105	42-43	0.19661252219189862
1105	44-45	0.2080095967527953
1105	46-47	0.15326117000370942
1105	48-49	0.13738642861642347
1105	50-51	0.17246453609316603
1105	52-53	0.0704161203131406
1105	54-55	-0.06967503945794107
1105	56-57	0.002538127050943473
1105	58-59	0.05090021636830144
1105	60-61	-0.02757677046711038
1105	62-63	0.05437870430730385
1105	64-65	-0.09414141879702953
1105	66-67	-0.07813376989734167
1105	68-69	-0.02592936281545022
1105	70-71	-0.04934350182812963
1105	72-73	-0.05340001976972886
1105	74-75	-0.03826742457028587
1105	76-77	-0.03908581495810992
1105	78-79	0.04089049690933777
1105	80-81	0.11980255086860225
1105	82-83	0.09461204270807144
1105	84-85	0.06287863325496446
1105	86-87	0.110143555525962
1105	88-89	0.13725966164579617
1105	90-91	0.1431965339446677
1105	92-93	0.2548133948562601
1105	94-95	0.27129654549794324
1105	96-97	0.24268154462696856
1105	98-99	0.2692968132877738
1105	100-101	0.273485379817199
1106	1	-0.15311666844008442
1106	2	-0.09126054325214739
1106	3	-0.13886523776511694
1106	4	-0.12504147688638056
1106	5	-0.20856513876906035
1106	6	-0.16734269593997908
1106	7	-0.22386977573459177
1106	8	-0.19849965888848686
1106	9	-0.15511405992273808
1106	10-11	-0.34305920896957076
1106	12-13	-0.3778277781544901
1106	14-15	-0.37691767832594536
1106	16-17	-0.3568329449658947
1106	18-19	-0.36460831574824937
1106	20-21	-0.1693003753797626
1106	22-23	-0.16694928938566989
1106	24-25	-0.28185971025638423
1106	26-27	-0.3031720681500829
1106	28-29	-0.33785604037679207
1106	30-31	-0.3410854567823449
1106	32-33	-0.3284664377415112
1106	34-35	-0.2712775799052807
1106	36-37	-0.3358709593645628
1106	38-39	-0.32608250008938455
1106	40-41	-0.3807461680856292
1106	42-43	-0.43831748429743556
1106	44-45	-0.3906159414072121
1106	46-47	-0.4296958010619605
1106	48-49	-0.6765393066380199
1106	50-51	-0.7308189456530201
1106	52-53	-0.8232837182518367
1106	54-55	-0.782432002139327
1106	56-57	-0.8624258470006332
1106	58-59	-0.7703709594910642
1106	60-61	-0.9182527357624259
1106	62-63	-0.798957694883704
1106	64-65	-0.7345134614715789
1106	66-67	-0.907528097176872
1106	68-69	-1.0162637623226018
1106	70-71	-0.8909251930359012
1106	72-73	-0.8986739890849123
1106	74-75	-0.9339453294898412
1106	76-77	-0.9854329303429914
1106	78-79	-0.9289355753442159
1106	80-81	-0.9208802887384095
1106	82-83	-1.0133564207610561
1106	84-85	-1.0551614204817845
1106	86-87	-0.9990924886573112
1106	88-89	-1.062109351498865
1106	90-91	-0.9838368428308613
1106	92-93	-0.984178818555197
1106	94-95	-1.0112178846185316
1106	96-97	-0.9984072438821983
1106	98-99	-1.0293360757740793
1106	100-101	-1.1508496622692057
1107	1	-0.43583619628248016
1107	2	-0.5018044558448871
1107	3	-0.47582221146738846
1107	4	-0.3157810303869084
1107	5	-0.3917707492022018
1107	6	-0.4018262669903905
1107	7	-0.4167836838842476
1107	8	-0.36893594295639787
1107	9	-0.4605123354441929
1107	10-11	-0.4655097920025
1107	12-13	-0.5411279114525414
1107	14-15	-0.6830149266133319
1107	16-17	-0.5815529446201424
1107	18-19	-0.5677411140250186
1107	20-21	-0.5106939299014073
1107	22-23	-0.5513552508322164
1107	24-25	-0.5729417284687912
1107	26-27	-0.4417747415723241
1107	28-29	-0.5140320632971935
1107	30-31	-0.5754304799445649
1107	32-33	-0.46007028539223427
1107	34-35	-0.5183747524794242
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2108	8	-0.8430381840586065
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2108	10-11	-1.6894578093424357
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2108	20-21	-1.2015278118332446
2108	22-23	-1.1643499329734368
2108	24-25	-1.2083340490726897
2108	26-27	-1.374265041443465
2108	28-29	-1.6296966661355654
2108	30-31	-1.839970376393012
2108	32-33	-1.7373303390586727
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2208	10-11	-0.9201140695976946
2208	12-13	-0.8662018332080379
2208	14-15	-1.2880231072762243
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2208	22-23	-1.0762235417175816
2208	24-25	-1.116856368084072
2208	26-27	-1.1180622810760212
2208	28-29	-1.1147077176625544
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2308	50-51	-2.5114501087834427
2308	52-53	-2.4397244419899877
2308	54-55	-2.210701283258878
2308	56-57	-2.079269275053189
2308	58-59	-1.9343498114512592
2308	60-61	-1.9261707324149633
2308	62-63	-2.022933087645619
2308	64-65	-2.0124891718069513
2308	66-67	-2.16109719822051
2308	68-69	-1.9632016437783122
2308	70-71	-1.9337978531370332
2308	72-73	-1.911008588007281
2308	74-75	-1.699310750680457
2308	76-77	-1.6297612024963861
2308	78-79	-1.713821278304824
2308	80-81	-2.29778366642617
2308	82-83	-2.094260674716317
2308	84-85	-1.8578969429548131
2308	86-87	-1.7609536737708744
2308	88-89	-1.6136480936962663
2308	90-91	-1.8352037060402147
2308	92-93	-1.851506137988224
2308	94-95	-1.8810453928313322
2308	96-97	-1.7969283595765617
2308	98-99	-1.8603378247804834
2308	100-101	-2.063781322813764
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101994.0
3	15924.0
4	13829.0
5	12994.0
6	14672.0
7	17798.0
8	21272.0
9	24586.0
10	28007.0
11	33244.0
12	32716.0
13	35953.0
14	40035.0
15	46184.0
16	58153.0
17	79570.0
18	123521.0
19	204025.0
20	297553.0
21	211738.0
22	113020.0
23	95513.0
24	100104.0
25	106702.0
26	114087.0
27	125931.0
28	143523.0
29	166929.0
30	198686.0
31	238377.0
32	292687.0
33	368010.0
34	487653.0
35	713028.0
36	1174343.0
37	1600434.0
38	1445165.0
39	268419.0
40	378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.004217694232558	46.798975057297206	24.33187824592294	17.864929002547296
2	16.875017948766384	48.40972564894277	18.340499918595864	16.374756483694984
3	43.80860469602942	20.39429500982047	19.18907767491155	16.60802261923856
4	16.875809088864973	18.851568622742466	20.087081429541325	44.18554085885124
5	16.936587170339696	46.12890236283439	20.997678066407783	15.936832400418135
6	44.06774298909328	19.996365082714004	18.939391790628765	16.996500137563945
7	17.70411286835182	19.486924549486158	47.029294472437236	15.779668109724781
8	43.617025222592396	20.16424064394097	19.867283505481925	16.35145062798471
9	43.219137677714855	19.84032733583486	20.41214386795721	16.52839111849308
10-11	16.50123567291478	33.177501370303006	33.50112635415612	16.820136602626093
12-13	16.3581999561709	20.152035808277308	33.409542360138715	30.08022187541308
14-15	16.53046834069449	46.80589368293322	19.805032426406427	16.858605549965862
16-17	29.945062092942315	19.97574024410193	19.95863470527282	30.12056295768294
18-19	16.677753893029173	33.25444639049691	19.929865747834146	30.137933968639768
20-21	30.10884012520034	33.29265187610699	19.95447022215512	16.644037776537544
22-23	30.01572862031811	19.91222277870329	33.37936029307728	16.692688307901324
24-25	16.614981315491914	33.107770148096066	20.37048834993239	29.906760186479627
26-27	16.831054543145775	25.4997500172637	40.72416229601425	16.945033143576275
28-29	28.181826264505283	19.792833093250252	35.19165764343744	16.83368299880703
30-31	26.215642156352835	20.86101773352577	35.55915483592494	17.36418527419645
32-33	20.273807293956988	23.53919970886048	36.907186950561155	19.279806046621378
34-35	19.709922110179438	22.526268914174576	37.63430643062844	20.12950254501754
36-37	19.54143595901873	22.512486467357366	37.79039818369136	20.155679389932544
38-39	19.801239880709456	22.61521184631868	37.52834634098607	20.0552019319858
40-41	20.035368127992914	22.292246339775126	37.464295045680196	20.208090486551768
42-43	20.457499715954043	22.65306722450038	37.376389554723396	19.513043504822182
44-45	19.9049625170413	22.888359266514588	37.313312022135776	19.89336619430833
46-47	20.00584072960902	22.47453626254178	37.39838041389762	20.12124259395158
48-49	20.019186111554905	22.47443456362669	37.6180497596336	19.888329565184808
50-51	20.097284056156248	22.639412861363727	36.71877463152467	20.544528450955358
52-53	20.542507224325007	23.659640501180736	35.44315647279804	20.354695801696217
54-55	21.196942002629847	24.533480391163486	33.66196665340619	20.607610952800478
56-57	21.497868524367856	25.09347171244699	32.64171630006087	20.766943463124285
58-59	21.42478413375229	25.908105646466105	31.757108741453262	20.910001478328347
60-61	21.770985350342748	26.15061507199129	31.24296078088113	20.835438796784835
62-63	21.79751569122909	25.97261484007285	31.4855237254704	20.74434574322766
64-65	21.154131378608643	25.805017697545413	31.532730411032155	21.508120512813793
66-67	21.296066820202253	25.297037840860746	32.4552787096894	20.951616629247603
68-69	21.18730991968213	25.042511955864583	32.61972564583681	21.150452478616486
70-71	20.88888422858652	25.0082055909866	33.25555361497653	20.84735656545036
72-73	20.653570943335065	24.80380224349579	32.930893479602815	21.61173333356633
74-75	21.126609190098097	25.076933663112744	32.184304385887785	21.61215276090137
76-77	21.57211301531602	25.63299433850731	31.72787738837822	21.067015257798445
78-79	21.60275707958494	25.40439339502214	31.509573833330318	21.483275692062602
80-81	21.311592081042928	25.87970311585035	31.474964443957237	21.333740359149488
82-83	22.23968541997856	26.105427972154676	30.727083493859624	20.927803114007133
84-85	21.738388423916255	26.689506861287814	30.1919819317291	21.38012278306683
86-87	21.56342382493005	26.77947137205275	30.21772279153226	21.439382011484938
88-89	21.55063945775573	26.30195573786284	30.263602252841554	21.883802551539876
90-91	21.522924281322663	26.19290557740998	30.297236336602268	21.98693380466509
92-93	21.42128554643691	26.74735083679018	30.26215199338856	21.56921162338436
94-95	21.534758939942364	26.211735753038983	29.827702174116368	22.425803132902292
96-97	21.532976533004124	26.119632800900796	30.292598158650698	22.054792507444386
98-99	21.978926651410564	26.50603587616775	29.651394613811853	21.86364285860983
100-101	22.09881927939046	26.363610346578685	29.51278302946651	22.02478734456434
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7761.0
1	4711.5
2	1199.0
3	640.5
4	526.5
5	503.5
6	607.5
7	809.0
8	823.5
9	900.5
10	1434.5
11	8299.0
12	151788.0
13	170654.0
14	47638.0
15	44100.5
16	47908.5
17	52066.0
18	55078.0
19	56940.0
20	58396.5
21	59906.0
22	61262.5
23	63552.5
24	67182.5
25	72073.5
26	79319.5
27	87132.5
28	94139.5
29	101585.0
30	110664.0
31	120326.0
32	129487.5
33	140924.0
34	161591.0
35	178241.5
36	187716.5
37	200354.0
38	208369.0
39	214351.5
40	224979.0
41	235064.0
42	241106.5
43	252360.5
44	267861.0
45	277331.0
46	282616.5
47	290388.0
48	298777.0
49	306735.5
50	311803.5
51	309494.0
52	309687.0
53	320006.0
54	308010.0
55	281343.5
56	273279.0
57	256737.0
58	249023.5
59	216402.5
60	156699.0
61	122950.5
62	93537.0
63	70047.5
64	51888.0
65	35738.0
66	23402.0
67	14860.5
68	9243.0
69	5630.5
70	3795.5
71	2758.0
72	2247.0
73	1928.5
74	1667.5
75	1519.0
76	1327.5
77	1139.0
78	1034.0
79	936.5
80	793.5
81	661.0
82	581.5
83	468.5
84	394.0
85	351.5
86	266.5
87	192.0
88	160.0
89	132.0
90	94.5
91	61.5
92	42.5
93	40.5
94	31.0
95	20.0
96	12.5
97	7.5
98	4.5
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05322493003796217
2	0.09540996886903405
3	0.08539552210230947
4	0.05691216642919628
5	0.08739186606561078
6	0.001210897158068006
7	0.0012327151248800422
8	0.00491995151611415
9	0.009599905397295902
10-11	0.009828994048822284
12-13	0.01760164472561016
14-15	0.011770793095093499
16-17	0.0014781672515154487
18-19	5.672671371129397E-4
20-21	9.818085065416265E-5
22-23	3.05451535368506E-4
24-25	7.854468052333012E-4
26-27	0.001352713942346241
28-29	0.001510894201733503
30-31	0.004565409555418563
32-33	1.963617013083253E-4
34-35	0.0023890673659179578
36-37	3.5999645239859635E-4
38-39	0.0025090661833841563
40-41	0.003403602822677638
42-43	0.0011618067327409246
44-45	6.327210375490481E-4
46-47	0.0010254444401656987
48-49	0.0025145206750871655
50-51	0.004407229296031301
52-53	0.13194960878749157
54-55	0.14169133096906572
56-57	0.1277932861097987
58-59	0.12158607455177442
60-61	0.10935164966192515
62-63	0.1139716041343738
64-65	0.1165624876933031
66-67	0.12482058813165876
68-69	0.13709864895513213
70-71	0.12451513659629027
72-73	0.11660612362692717
74-75	0.12154789310985334
76-77	0.13300778017787535
78-79	0.14670946333583404
80-81	0.13686956030360573
82-83	0.11646976133435194
84-85	0.1228351531517635
86-87	0.12410604971856459
88-89	0.12033699595178536
90-91	0.13077689307134463
92-93	0.14766945387556363
94-95	0.1565330028929533
96-97	0.1570075436711151
98-99	0.13990771218218176
100-101	0.1300787181333595
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	9166757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.33668706451326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.89858294772685	45.33971628564214
2	4.612290547935303	4.551102708281928
3	1.16476729884813	1.7239727917874572
4	0.5533285268464929	1.0919758549157417
5	0.32144688689172285	0.7929562233219458
6	0.22350842287591435	0.661629906942713
7	0.17302653064801352	0.5975589057507619
8	0.12959179730551357	0.5114903959831965
9	0.10923534914894968	0.48503792136101165
>10	0.7763069839566359	6.963818674022612
>50	0.02138763886763761	0.7110802368388981
>100	0.010304176769090618	1.1773000812422734
>500	0.0026895550947165486	0.904372870758642
>1k	0.003005973341051629	3.188318304692191
>5k	3.164182464264873E-4	1.1689277586677607
>10k+	2.109454976176582E-4	30.130741079790603
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1102554	12.027743290238849	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	25469	0.2778408983678743	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC	19550	0.21327062558765328	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG	10483	0.11435887304528744	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.181796681203614E-5	0.0	0.0	0.0	0.0
3	8.727186724814457E-5	0.0	0.0	0.0	0.0
4	1.6363475109027107E-4	0.0	0.0	0.0	0.0
5	1.9636170130832528E-4	0.0	0.0	0.0	0.0
6	2.1817966812036143E-4	0.0	0.0	0.0	1.090898340601807E-5
7	2.290886515263795E-4	0.0	0.0	0.0	1.090898340601807E-5
8	2.290886515263795E-4	0.0	0.0	0.0	1.090898340601807E-5
9	2.8363356855646985E-4	0.0	0.0	0.0	1.090898340601807E-5
10-11	3.163605187745241E-4	0.0	0.0	0.0	1.090898340601807E-5
12-13	3.545419606955873E-4	0.0	0.0	0.0	1.090898340601807E-5
14-15	4.2545035283470477E-4	0.0	0.0	0.0	1.090898340601807E-5
16-17	4.5817730305275904E-4	0.0	0.0	0.0	1.090898340601807E-5
18-19	4.909042532708132E-4	0.0	0.0	0.0	1.090898340601807E-5
20-21	5.127222200828494E-4	0.0	0.0	0.0	1.090898340601807E-5
22-23	5.236312034888675E-4	0.0	0.0	0.0	1.090898340601807E-5
24-25	5.672671371129397E-4	0.0	0.0	0.0	1.090898340601807E-5
26-27	6.10903070737012E-4	0.0	0.0	0.0	1.090898340601807E-5
28-29	6.545390043610843E-4	0.0	0.0	0.0	1.090898340601807E-5
30-31	6.872659545791384E-4	0.0	0.0	0.0	1.090898340601807E-5
32-33	7.363563799062198E-4	0.0	0.0	0.0	1.090898340601807E-5
34-35	7.909012969363102E-4	0.0	0.0	0.0	1.090898340601807E-5
36-37	8.399917222633915E-4	0.0	0.0	0.0	1.090898340601807E-5
38-39	9.054456226995E-4	0.0	0.0	0.0	1.090898340601807E-5
40-41	9.818085065416264E-4	0.0	0.0	0.0	1.090898340601807E-5
42-43	0.001047262406977735	0.0	0.0	0.0	1.090898340601807E-5
44-45	0.0010799893571957892	0.0	0.0	0.0	1.090898340601807E-5
46-47	0.0011508977493349065	0.0	0.0	0.0	2.181796681203614E-5
48-49	0.001221806141474024	0.0	0.0	0.0	2.181796681203614E-5
50-51	0.0012981690253161505	0.0	0.0	1.090898340601807E-5	2.181796681203614E-5
52-53	0.0014127133510793404	0.0	0.0	1.090898340601807E-5	2.181796681203614E-5
54-55	0.0014890762349214668	0.0	0.0	1.090898340601807E-5	2.727245851504518E-5
56-57	0.0016036205606846565	0.0	0.0	1.090898340601807E-5	3.2726950218054216E-5
58-59	0.0017563463283689095	0.0	0.0	2.181796681203614E-5	3.2726950218054216E-5
60-61	0.0018927086209441354	0.0	0.0	2.181796681203614E-5	3.2726950218054216E-5
62-63	0.00203452540522237	0.0	0.0	2.181796681203614E-5	4.363593362407228E-5
64-65	0.002116342780767506	0.0	0.0	3.2726950218054216E-5	6.545390043610843E-5
66-67	0.002290886515263795	0.0	0.0	3.2726950218054216E-5	6.545390043610843E-5
68-69	0.0025308841501961925	0.0	0.0	3.2726950218054216E-5	8.727186724814457E-5
70-71	0.0027763362768315992	0.0	0.0	3.2726950218054216E-5	8.727186724814457E-5
72-73	0.0029672434864369155	0.0	0.0	3.2726950218054216E-5	1.0908983406018072E-4
74-75	0.0031690596794482495	0.0	0.0	3.2726950218054216E-5	1.1999881746619879E-4
76-77	0.0034636022314107374	0.0	0.0	3.2726950218054216E-5	1.1999881746619879E-4
78-79	0.003796326225294289	0.0	0.0	4.363593362407228E-5	1.1999881746619879E-4
80-81	0.0042763214951590835	0.0	0.0	4.363593362407228E-5	1.1999881746619879E-4
82-83	0.004854497615678042	0.0	0.0	4.363593362407228E-5	1.1999881746619879E-4
84-85	0.005558127045366207	0.0	0.0	4.363593362407228E-5	1.1999881746619879E-4
86-87	0.006758115220028196	0.0	0.0	5.454491703009036E-5	1.1999881746619879E-4
88-89	0.00814355611259249	0.0	0.0	5.454491703009036E-5	1.2545330916920782E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGTA	248615	0.0	91.843414	5
GGTATCA	249755	0.0	91.57785	8
GTATCAA	250315	0.0	91.42797	9
TGGTATC	250140	0.0	91.36479	7
AAGCAGT	250530	0.0	91.3356	1
GTGGTAT	252140	0.0	90.714294	6
GCAGTGG	252265	0.0	90.57856	3
AGCAGTG	253330	0.0	90.33477	2
CAGTGGT	253100	0.0	90.18352	4
TCAACGC	249200	0.0	46.4042	12-13
ATCAACG	249090	0.0	46.28335	10-11
AACGCAG	249920	0.0	46.276417	14-15
CAACGCA	249230	0.0	46.260536	12-13
CGCAGAG	250845	0.0	46.086426	16-17
ACGCAGA	250540	0.0	46.05676	14-15
TATCAAC	251075	0.0	45.994953	10-11
CAGAGTA	249650	0.0	45.77628	18-19
AGAGTAC	245105	0.0	45.71896	18-19
GAGTACT	137710	0.0	45.471447	20-21
GCAGAGT	253105	0.0	45.434914	16-17
>>END_MODULE