FastQCFastQC Report
Sat 18 Jun 2016
SRR578560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9166757
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105570.11516613781733277No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102690.11202435059639956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4100.032.382533
CTCGCGT2700.021.94717252-53
CAACGCA69450.020.85313412-13
ATCAACG69750.020.55954610-11
CGCGTAG3000.020.54255754-55
CGTACGC3300.018.67764920-21
TCGCGTA3450.018.55013352-53
GGTATCA88550.018.1515878
AGTGGTA89000.018.006735
TCAACGC67450.017.85188710-11
ACTCGCG3500.017.60790650-51
GTATCAA93100.017.415939
CGCTAGT4100.017.18914490-91
AACGCAG73450.016.32905812-13
ACGTACG3700.016.01773320-21
AAGCAGT102550.015.9081111
TGGTATC102950.015.5666017
ACGCAGA94550.015.34236314-15
CGTAGCG4050.015.21670656-57
AGAGTAC101650.014.13179118-19
Produced by FastQC (version 0.11.5)