##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578560_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9166757 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.996707123358892 31.0 31.0 34.0 30.0 34.0 2 32.269978248577985 34.0 31.0 34.0 30.0 34.0 3 32.34075507837723 34.0 31.0 34.0 30.0 34.0 4 35.907940289024786 37.0 35.0 37.0 35.0 37.0 5 35.784023182898814 37.0 35.0 37.0 33.0 37.0 6 35.758894994162056 37.0 35.0 37.0 33.0 37.0 7 35.75226735038356 37.0 35.0 37.0 33.0 37.0 8 35.67018826832652 37.0 35.0 37.0 33.0 37.0 9 37.306574833389824 39.0 37.0 39.0 33.0 39.0 10-11 37.29971728278605 39.0 37.0 39.0 33.5 39.0 12-13 37.29545628841258 39.0 37.0 39.0 33.0 39.0 14-15 38.607057435906725 40.0 38.0 41.0 34.0 41.0 16-17 38.5652754840125 40.0 38.0 41.0 34.0 41.0 18-19 38.51807318553333 40.0 38.0 41.0 34.0 41.0 20-21 38.45539545773931 40.0 38.0 41.0 34.0 41.0 22-23 38.337721453726765 40.0 38.0 41.0 33.5 41.0 24-25 38.25406793263964 40.0 38.0 41.0 33.0 41.0 26-27 38.08630527677346 40.0 37.0 41.0 33.0 41.0 28-29 37.99076434555863 40.0 37.0 41.0 33.0 41.0 30-31 37.89254389529471 40.0 37.0 41.0 33.0 41.0 32-33 37.75879337698163 40.0 37.0 41.0 32.5 41.0 34-35 37.62704214805738 40.0 37.0 41.0 32.0 41.0 36-37 37.50129893265415 40.0 37.0 41.0 31.5 41.0 38-39 37.371384394721055 40.0 36.0 41.0 31.0 41.0 40-41 37.22489125652616 39.5 36.0 41.0 31.0 41.0 42-43 37.065277065815096 39.0 36.0 41.0 31.0 41.0 44-45 36.90451928637358 39.0 35.5 41.0 30.0 41.0 46-47 36.9918951707785 39.0 36.0 41.0 31.0 41.0 48-49 36.97229778208367 39.0 36.0 41.0 31.0 41.0 50-51 36.94596867790867 39.0 36.0 41.0 31.0 41.0 52-53 36.957159385811146 39.0 36.0 41.0 31.0 41.0 54-55 36.82919624682971 39.0 35.0 41.0 31.0 41.0 56-57 36.67528194540338 39.0 35.0 41.0 30.0 41.0 58-59 36.45231148813043 39.0 35.0 41.0 30.0 41.0 60-61 36.219669017079866 38.5 35.0 40.5 30.0 41.0 62-63 35.941317196474174 38.0 35.0 40.0 29.0 41.0 64-65 35.62242655717829 37.5 34.0 40.0 29.0 41.0 66-67 35.2981608435786 37.0 34.0 40.0 29.0 41.0 68-69 34.95913200273554 36.5 34.0 39.0 28.0 41.0 70-71 34.58270165774003 36.0 34.0 39.0 28.0 41.0 72-73 34.15510305334809 35.5 33.5 39.0 28.0 40.0 74-75 33.76536134862089 35.0 33.0 37.5 27.0 40.0 76-77 32.3299479303313 34.5 31.5 36.0 25.5 39.0 78-79 32.9285587040215 35.0 32.5 36.0 26.0 39.0 80-81 32.87750438895675 35.0 33.0 36.0 26.0 38.5 82-83 32.606584640565906 35.0 33.0 36.0 26.0 37.0 84-85 32.328910704189056 35.0 33.0 35.5 26.0 37.0 86-87 32.05521538315023 35.0 32.0 35.0 26.0 36.5 88-89 31.809464350369492 35.0 32.0 35.0 25.0 36.0 90-91 31.57590765196459 35.0 32.0 35.0 25.0 36.0 92-93 31.339354637632482 35.0 32.0 35.0 24.5 36.0 94-95 31.092181237050355 35.0 32.0 35.0 24.0 35.5 96-97 30.490326349874877 34.0 31.5 35.0 18.5 35.0 98-99 30.463150490407894 34.0 31.0 35.0 19.0 35.0 100-101 29.878698977184627 34.0 30.5 35.0 9.5 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.428134796067134 1101 2 0.4195658126644304 1101 3 0.39399583781882086 1101 4 0.2964117904197039 1101 5 0.2875078003369893 1101 6 0.3303796179066367 1101 7 0.34050123452701797 1101 8 0.4244947431006594 1101 9 0.5087837168196359 1101 10-11 0.46516423050870515 1101 12-13 0.4735236033003005 1101 14-15 0.5942295966408366 1101 16-17 0.602997205213633 1101 18-19 0.5749200098681655 1101 20-21 0.6477708675464555 1101 22-23 0.664493012517319 1101 24-25 0.7041549491170613 1101 26-27 0.7308422681099671 1101 28-29 0.7540467714637202 1101 30-31 0.7381579926643056 1101 32-33 0.6948472194312174 1101 34-35 0.7281132944093471 1101 36-37 0.7551338573063759 1101 38-39 0.7848284839015349 1101 40-41 0.7668252312987036 1101 42-43 0.7753982935884522 1101 44-45 0.7476709701450588 1101 46-47 0.8026627352507134 1101 48-49 0.7664652480990668 1101 50-51 0.7544549173538115 1101 52-53 0.720214072817619 1101 54-55 0.8177894073598537 1101 56-57 0.8418588648693728 1101 58-59 0.8372620854867634 1101 60-61 0.7870675137712979 1101 62-63 0.8066164403451168 1101 64-65 0.8164604933108279 1101 66-67 0.7853514495038496 1101 68-69 0.7838921411693249 1101 70-71 0.7500713669785526 1101 72-73 0.7332186233882183 1101 74-75 0.797060497439773 1101 76-77 0.7542588729591486 1101 78-79 0.6929375190474403 1101 80-81 0.5985795856471228 1101 82-83 0.606111247611409 1101 84-85 0.5758453606269285 1101 86-87 0.5489742066244148 1101 88-89 0.5630688968288133 1101 90-91 0.5606935022864334 1101 92-93 0.6276243797836827 1101 94-95 0.6839702635396527 1101 96-97 1.0479182494893564 1101 98-99 0.7539966026696163 1101 100-101 0.7357161116954636 1102 1 0.4033388657600554 1102 2 0.37263370838694954 1102 3 0.3642612594009549 1102 4 0.24879007641214912 1102 5 0.3175778512600971 1102 6 0.29256519535240955 1102 7 0.2538522677693962 1102 8 0.29638361291605975 1102 9 0.4541302604100039 1102 10-11 0.3957424668045135 1102 12-13 0.4175758890795791 1102 14-15 0.49362810987860684 1102 16-17 0.5244407172460654 1102 18-19 0.534018546011481 1102 20-21 0.5628773596689243 1102 22-23 0.571543360738417 1102 24-25 0.6168911232380907 1102 26-27 0.6640923884650576 1102 28-29 0.6582021280151977 1102 30-31 0.7001976077543333 1102 32-33 0.7214180168368998 1102 34-35 0.7469802070795311 1102 36-37 0.7870049329718256 1102 38-39 0.8235212249014481 1102 40-41 0.8113036539655525 1102 42-43 0.7921440907483941 1102 44-45 0.8219959160960641 1102 46-47 0.8982579396402102 1102 48-49 0.8901990187488025 1102 50-51 0.9054525422706519 1102 52-53 0.8053064956231779 1102 54-55 0.8152835021921163 1102 56-57 0.7403221626825811 1102 58-59 0.7974960700034899 1102 60-61 0.8518033900292821 1102 62-63 0.8241153729413853 1102 64-65 0.7669457151883776 1102 66-67 0.7755157940495891 1102 68-69 0.8013145078607877 1102 70-71 0.7560901693516513 1102 72-73 0.8217999341775766 1102 74-75 0.706411047852022 1102 76-77 0.7000661734503382 1102 78-79 0.7372232692804346 1102 80-81 0.7383731212117723 1102 82-83 0.7331360486303353 1102 84-85 0.7272401201800491 1102 86-87 0.6924801578118362 1102 88-89 0.6204513177373805 1102 90-91 0.6424880052880155 1102 92-93 0.6616261109195278 1102 94-95 0.7325683654313302 1102 96-97 1.1415859039667566 1102 98-99 0.9584224701981547 1102 100-101 0.9400193646654458 1103 1 0.3038173603478427 1103 2 0.2772346198260962 1103 3 0.24763575731553544 1103 4 0.21962928862557618 1103 5 0.2004932590905426 1103 6 0.26352735480380574 1103 7 0.264064604267368 1103 8 0.055929470246951496 1103 9 0.1632596716677739 1103 10-11 0.3726891041986633 1103 12-13 0.32883036871168514 1103 14-15 0.4369524093847801 1103 16-17 0.42447375521550157 1103 18-19 0.35952840868996816 1103 20-21 0.37806227884766486 1103 22-23 0.39872162746720363 1103 24-25 0.26153794305476197 1103 26-27 0.24064892010253658 1103 28-29 0.4468310447128445 1103 30-31 0.25101309767421043 1103 32-33 0.49190615784302594 1103 34-35 0.452277360563599 1103 36-37 0.5096033472660082 1103 38-39 0.5630311917078075 1103 40-41 0.46066491962687195 1103 42-43 0.5783622466732581 1103 44-45 0.6433765546535994 1103 46-47 0.5006633331427466 1103 48-49 0.4782121283261134 1103 50-51 0.47181070840042594 1103 52-53 0.5247522687417074 1103 54-55 0.639565564341197 1103 56-57 0.5817839011194863 1103 58-59 0.3538579373889519 1103 60-61 0.29111575990589955 1103 62-63 0.1271267963946059 1103 64-65 0.3721353481962666 1103 66-67 0.33603725960551856 1103 68-69 0.29933192055988656 1103 70-71 0.32131678147941045 1103 72-73 0.5075437625225945 1103 74-75 0.42898365887222667 1103 76-77 0.29166776768258984 1103 78-79 0.40813148317070613 1103 80-81 0.41870323183290026 1103 82-83 0.427317299943077 1103 84-85 0.46765446808108635 1103 86-87 0.31348672460311633 1103 88-89 0.26672131490012063 1103 90-91 0.4065825539725836 1103 92-93 0.38652755574041464 1103 94-95 0.40152112331159273 1103 96-97 0.5202249522347628 1103 98-99 0.2797698941379174 1103 100-101 0.524537468098945 1104 1 0.3463530426431163 1104 2 0.3258600843955435 1104 3 0.2878154914932409 1104 4 0.19302898698807525 1104 5 0.221504261618648 1104 6 0.2390902930352823 1104 7 0.23905553066020957 1104 8 0.3190933522622714 1104 9 0.3195712176501786 1104 10-11 0.3110356051016723 1104 12-13 0.30334487442583935 1104 14-15 0.38809756525211725 1104 16-17 0.4269137780210883 1104 18-19 0.4361874279412703 1104 20-21 0.40728842800756127 1104 22-23 0.5217330290655084 1104 24-25 0.47095898395077995 1104 26-27 0.5790688739428802 1104 28-29 0.5786118329211973 1104 30-31 0.5644782786010936 1104 32-33 0.5924565829995458 1104 34-35 0.5663233580602878 1104 36-37 0.6157244892134486 1104 38-39 0.60091886607389 1104 40-41 0.5720262003136938 1104 42-43 0.6612977661047452 1104 44-45 0.6274556749146427 1104 46-47 0.4576179538923171 1104 48-49 0.6382585073662455 1104 50-51 0.5661430614318164 1104 52-53 0.6126695930055064 1104 54-55 0.6805659356198674 1104 56-57 0.6665797268036471 1104 58-59 0.6855447798664969 1104 60-61 0.588170342161014 1104 62-63 0.4451930154493908 1104 64-65 0.5942895011291967 1104 66-67 0.6473249923674871 1104 68-69 0.6980829862203777 1104 70-71 0.6204437664161944 1104 72-73 0.6926726068249494 1104 74-75 0.6506722978397335 1104 76-77 0.5502736822864165 1104 78-79 0.5321027950370478 1104 80-81 0.6729068804387381 1104 82-83 0.7355805715470751 1104 84-85 0.728909785582573 1104 86-87 0.677678043827612 1104 88-89 0.694214274102702 1104 90-91 0.7091588164725238 1104 92-93 0.7305748511957262 1104 94-95 0.7408639530078673 1104 96-97 1.0929575776903846 1104 98-99 0.8594838333709625 1104 100-101 0.8894507385094741 1105 1 0.17017946584463317 1105 2 0.16543872152398365 1105 3 0.1510121263736508 1105 4 0.08453586074270447 1105 5 0.10980579584466454 1105 6 0.08445163201931649 1105 7 0.10287782079421959 1105 8 0.18566252714630593 1105 9 0.18159644995920132 1105 10-11 0.1622492682826291 1105 12-13 0.17412242996084615 1105 14-15 0.2025601178300107 1105 16-17 0.18220000175491435 1105 18-19 0.1465782029017717 1105 20-21 0.2000267625016079 1105 22-23 0.18736229468837706 1105 24-25 0.21339264911533462 1105 26-27 0.1363743509117583 1105 28-29 0.2196239064791925 1105 30-31 0.1754847923064844 1105 32-33 0.19030736652040758 1105 34-35 0.17999769620646333 1105 36-37 0.22653193197415078 1105 38-39 0.22292610680894143 1105 40-41 0.2549540611597436 1105 42-43 0.1963777932281019 1105 44-45 0.2686154665328644 1105 46-47 0.2693656149296473 1105 48-49 0.24594720857473362 1105 50-51 0.19055326135910633 1105 52-53 0.27489889999949213 1105 54-55 0.32458654772591444 1105 56-57 0.3297766363323902 1105 58-59 0.27053536895910213 1105 60-61 0.3026715023235056 1105 62-63 0.34732611547200065 1105 64-65 0.27498205667323816 1105 66-67 0.30311278084880655 1105 68-69 0.28083929236124305 1105 70-71 0.2854742654636482 1105 72-73 0.29825712637192936 1105 74-75 0.3294107651584497 1105 76-77 0.3418413621998013 1105 78-79 0.3003243025151363 1105 80-81 0.2847607795517888 1105 82-83 0.30744772257209974 1105 84-85 0.3435457307857277 1105 86-87 0.24904155450307286 1105 88-89 0.24453369675505954 1105 90-91 0.29564851471394604 1105 92-93 0.29756550123657277 1105 94-95 0.354752945720179 1105 96-97 0.7247246663861233 1105 98-99 0.508951394800885 1105 100-101 0.45728304850774393 1106 1 -0.05364208736771303 1106 2 -0.08613729322257058 1106 3 -0.08564292773948523 1106 4 -0.05534085078276263 1106 5 -0.026231030867762684 1106 6 -0.0488587237047966 1106 7 -0.044966740339923206 1106 8 -0.04481263848111183 1106 9 0.0027389876067118735 1106 10-11 -0.07689170475077134 1106 12-13 -0.11764145312832852 1106 14-15 -0.18210805069715974 1106 16-17 -0.13212344592643888 1106 18-19 -0.14536397863059136 1106 20-21 -0.15049940639538306 1106 22-23 -0.13036762353255682 1106 24-25 -0.057573934737760624 1106 26-27 -0.07932345192948276 1106 28-29 -0.0835864073677115 1106 30-31 -0.22448022870106144 1106 32-33 -0.2671589570383688 1106 34-35 -0.3340038528709073 1106 36-37 -0.22518416428916765 1106 38-39 -0.3196710108766396 1106 40-41 -0.2871112729135561 1106 42-43 -0.1944976201624442 1106 44-45 -0.20817768798323044 1106 46-47 -0.2599275670147918 1106 48-49 -0.30153345566916556 1106 50-51 -0.29085073066489997 1106 52-53 -0.1909220695210223 1106 54-55 -0.21519657887062493 1106 56-57 -0.20220613613144423 1106 58-59 -0.18188115159694718 1106 60-61 -0.2753104510633406 1106 62-63 -0.18080464149414155 1106 64-65 -0.11414151644952142 1106 66-67 -0.15396149589632557 1106 68-69 -0.17788272157857676 1106 70-71 -0.1454138269729981 1106 72-73 -0.14543764784428248 1106 74-75 -0.13054454405227034 1106 76-77 -0.19867541761409768 1106 78-79 -0.1306150516397011 1106 80-81 -0.1743053368491303 1106 82-83 -0.17116070445900533 1106 84-85 -0.20608664417570566 1106 86-87 -0.26032391372597985 1106 88-89 -0.27143751022816787 1106 90-91 -0.2058238352476316 1106 92-93 -0.14500848280406586 1106 94-95 -0.08863978241686965 1106 96-97 0.1710125807394931 1106 98-99 -0.03196460381440147 1106 100-101 -0.019185606980432368 1107 1 -0.19896232307289452 1107 2 -0.25862352673134126 1107 3 -0.18808707990187656 1107 4 -0.1615587421560818 1107 5 -0.17948125411165705 1107 6 -0.1322708445709324 1107 7 -0.16903235715836473 1107 8 -0.16657836553429917 1107 9 -0.24175660745223837 1107 10-11 -0.26512389857298047 1107 12-13 -0.1957696493845802 1107 14-15 -0.2584478158614374 1107 16-17 -0.303604084988379 1107 18-19 -0.2891154208520277 1107 20-21 -0.29601236907016926 1107 22-23 -0.2521138220117294 1107 24-25 -0.2373415792634006 1107 26-27 -0.2091053019861704 1107 28-29 -0.18729231350244646 1107 30-31 -0.27059872523513917 1107 32-33 -0.33603112944279445 1107 34-35 -0.33283282932194425 1107 36-37 -0.37178768812712804 1107 38-39 -0.3793993745795845 1107 40-41 -0.33951116022972627 1107 42-43 -0.38651228997262166 1107 44-45 -0.3121418133713263 1107 46-47 -0.33189938502268745 1107 48-49 -0.45691478869321145 1107 50-51 -0.4192249709466438 1107 52-53 -0.41837130979970283 1107 54-55 -0.39400559545240554 1107 56-57 -0.3752892670017687 1107 58-59 -0.3192000966931374 1107 60-61 -0.40059183225064743 1107 62-63 -0.5419691493824033 1107 64-65 -0.4574716812387578 1107 66-67 -0.40448312138627784 1107 68-69 -0.45452636180608863 1107 70-71 -0.3944614523628971 1107 72-73 -0.39796643928514897 1107 74-75 -0.38561995358431034 1107 76-77 -0.44669479508176835 1107 78-79 -0.26971213389654736 1107 80-81 -0.3218183964888439 1107 82-83 -0.31284073369838694 1107 84-85 -0.3289902037347545 1107 86-87 -0.32025219108325587 1107 88-89 -0.3608872230719733 1107 90-91 -0.2986218722888552 1107 92-93 -0.28907045077341564 1107 94-95 -0.2809393490755525 1107 96-97 0.034398294435099785 1107 98-99 -0.21091570748532007 1107 100-101 -0.3138698667548745 1108 1 -0.34043322045287994 1108 2 -0.34695203564223576 1108 3 -0.32883867729273675 1108 4 -0.23914026725660165 1108 5 -0.27054026140850596 1108 6 -0.2674965206779376 1108 7 -0.30661654798709037 1108 8 -0.28331674184860134 1108 9 -0.34995694160360813 1108 10-11 -0.40607733601780893 1108 12-13 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-0.14086552083160342 2305 24-25 -0.06130898835040455 2305 26-27 -0.1297674170264571 2305 28-29 -0.1269702801377477 2305 30-31 -0.16370348823792114 2305 32-33 -0.1754154447874683 2305 34-35 -0.17810487145136733 2305 36-37 -0.0788188988902192 2305 38-39 -0.1816037376888957 2305 40-41 -0.1782535187712142 2305 42-43 -0.20712411933956787 2305 44-45 -0.1457239620436468 2305 46-47 -0.031188715318322124 2305 48-49 -0.10852293020090542 2305 50-51 -0.11652056547112011 2305 52-53 -0.16871205455598925 2305 54-55 -0.07662842250525159 2305 56-57 -0.11904964563872511 2305 58-59 -0.05039871770151905 2305 60-61 -0.07685916746392962 2305 62-63 -0.09000075738870805 2305 64-65 -0.025348070661536326 2305 66-67 -0.10336415673009469 2305 68-69 -0.0907749190063214 2305 70-71 -0.08046167915329505 2305 72-73 -0.07119760572213352 2305 74-75 -0.1115904443424185 2305 76-77 -0.15271308482303425 2305 78-79 -0.0940444251549053 2305 80-81 -0.0874903474495099 2305 82-83 -0.04701442910862852 2305 84-85 -0.012517361250495185 2305 86-87 -0.01900005530686144 2305 88-89 0.019381449076966817 2305 90-91 -0.01692483904646025 2305 92-93 0.0036581998596929566 2305 94-95 -0.08979794557600229 2305 96-97 0.16985944240187223 2305 98-99 -0.13262092294122851 2305 100-101 -0.08430603547231286 2306 1 -0.27053403377321317 2306 2 -0.28685403680596266 2306 3 -0.27881550111704456 2306 4 -0.19111296067335104 2306 5 -0.2359591266469394 2306 6 -0.1843486911907135 2306 7 -0.22875147229221682 2306 8 -0.18599126268971844 2306 9 -0.39447231297150154 2306 10-11 -0.3084884403515744 2306 12-13 -0.31286920611827895 2306 14-15 -0.36399012521813034 2306 16-17 -0.4445460852307974 2306 18-19 -0.457176306461065 2306 20-21 -0.4758297336605821 2306 22-23 -0.41901894319114064 2306 24-25 -0.42839365924993444 2306 26-27 -0.45244316020663433 2306 28-29 -0.581857971934312 2306 30-31 -0.5625626338946219 2306 32-33 -0.6111948210700575 2306 34-35 -0.630122805270318 2306 36-37 -0.6388249275030731 2306 38-39 -0.790623148350079 2306 40-41 -0.6391158737555784 2306 42-43 -0.7729125589499617 2306 44-45 -0.7843104936154859 2306 46-47 -0.703249189329874 2306 48-49 -0.6617964384979871 2306 50-51 -0.6786911942148492 2306 52-53 -0.5538831532684227 2306 54-55 -0.5428863546118095 2306 56-57 -0.5810670994979077 2306 58-59 -0.6226687592959053 2306 60-61 -0.604737124365073 2306 62-63 -0.5723914843733127 2306 64-65 -0.6419025970440089 2306 66-67 -0.5457902907905208 2306 68-69 -0.6278789150356445 2306 70-71 -0.6889056518934709 2306 72-73 -0.6665892261045343 2306 74-75 -0.6301017768029951 2306 76-77 -0.6488096910647734 2306 78-79 -0.5906200569761211 2306 80-81 -0.5674874604089482 2306 82-83 -0.5319590417580358 2306 84-85 -0.5052755714460346 2306 86-87 -0.5201081091652711 2306 88-89 -0.4756332511372783 2306 90-91 -0.5340071887151723 2306 92-93 -0.5713344295348293 2306 94-95 -0.5963659484633403 2306 96-97 -0.3506023084104619 2306 98-99 -0.5795838206423163 2306 100-101 -0.5751601537222655 2307 1 -0.4584519271659566 2307 2 -0.46439894866782794 2307 3 -0.45991744559745484 2307 4 -0.3899528253497593 2307 5 -0.42443906728252045 2307 6 -0.368628784543219 2307 7 -0.41268045246617646 2307 8 -0.3996297589565003 2307 9 -0.4446185784008918 2307 10-11 -0.5918847423724998 2307 12-13 -0.5968773989500846 2307 14-15 -0.8998441805803097 2307 16-17 -0.7995314208394078 2307 18-19 -0.7226859086864152 2307 20-21 -0.6230398890324693 2307 22-23 -0.5995591562084073 2307 24-25 -0.6287085387393887 2307 26-27 -0.7349093523314636 2307 28-29 -0.7347386068313853 2307 30-31 -0.7660988597895866 2307 32-33 -0.8117475988257397 2307 34-35 -0.7912167852456875 2307 36-37 -0.864513494302301 2307 38-39 -0.8932909593542107 2307 40-41 -0.7956234221881786 2307 42-43 -0.9110105722867914 2307 44-45 -0.8201136317586801 2307 46-47 -0.8680134258584005 2307 48-49 -0.7506044106214418 2307 50-51 -0.8183651692362304 2307 52-53 -0.8978237074896995 2307 54-55 -0.9878924976883425 2307 56-57 -1.005567742361066 2307 58-59 -1.0113461556165078 2307 60-61 -1.0937743396846358 2307 62-63 -1.068808697665709 2307 64-65 -1.0561870125407609 2307 66-67 -0.9892402332242227 2307 68-69 -0.9815078889208522 2307 70-71 -1.0184058096626316 2307 72-73 -1.3520908804709322 2307 74-75 -1.0422926970304047 2307 76-77 -0.978723627363614 2307 78-79 -0.9930042055684396 2307 80-81 -0.9117176432481209 2307 82-83 -0.9461109899316078 2307 84-85 -0.9664698342533136 2307 86-87 -0.8968944954374081 2307 88-89 -1.0095146353051163 2307 90-91 -1.0324579487588679 2307 92-93 -1.0019325322993389 2307 94-95 -1.0656188671580331 2307 96-97 -0.8365540392793385 2307 98-99 -1.1848858206850714 2307 100-101 -1.1620955974124527 2308 1 -1.3823622404616458 2308 2 -0.8649199047820062 2308 3 -0.8740476739336032 2308 4 -0.5362484898358346 2308 5 -0.6252153015720836 2308 6 -0.6216136522684153 2308 7 -0.5830891637312092 2308 8 -0.6092746796315751 2308 9 -0.8242829237855887 2308 10-11 -0.8247786650670292 2308 12-13 -0.7884514442245134 2308 14-15 -1.027231937278735 2308 16-17 -0.9399934337057445 2308 18-19 -0.9729785961839355 2308 20-21 -0.9567875749022079 2308 22-23 -1.0623501240589022 2308 24-25 -1.1758640227997006 2308 26-27 -1.1833937426973833 2308 28-29 -1.2315441371644553 2308 30-31 -1.1309703633404453 2308 32-33 -1.175408729738102 2308 34-35 -1.241157570185841 2308 36-37 -1.2629448903326619 2308 38-39 -1.2885724313709446 2308 40-41 -1.247531160304355 2308 42-43 -1.1386829054551484 2308 44-45 -1.1609516309635026 2308 46-47 -1.1880591341692295 2308 48-49 -1.2373627070073852 2308 50-51 -1.2862438971898342 2308 52-53 -1.2473385377042732 2308 54-55 -1.207691036037609 2308 56-57 -1.1103917919265243 2308 58-59 -1.0709327278786773 2308 60-61 -1.0306287455292136 2308 62-63 -1.0384772091461016 2308 64-65 -1.046950740664613 2308 66-67 -0.9986902528785322 2308 68-69 -1.0553178889574326 2308 70-71 -1.0186613084390572 2308 72-73 -1.0586438400561988 2308 74-75 -1.22597229315042 2308 76-77 -1.1596730075844235 2308 78-79 -1.5480241156566308 2308 80-81 -1.2006322022697304 2308 82-83 -1.0969630879066088 2308 84-85 -1.0678152800095155 2308 86-87 -1.0469683984748102 2308 88-89 -1.069785330792108 2308 90-91 -1.1236690028970102 2308 92-93 -1.0607134335390107 2308 94-95 -1.1874715084756495 2308 96-97 -0.9863749376827116 2308 98-99 -1.279515400864966 2308 100-101 -1.2852959614041701 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36.0 3 58.0 4 164.0 5 424.0 6 847.0 7 1770.0 8 3476.0 9 5488.0 10 7495.0 11 9305.0 12 11284.0 13 13073.0 14 14444.0 15 16498.0 16 18999.0 17 22038.0 18 25706.0 19 29831.0 20 32124.0 21 32791.0 22 36057.0 23 42040.0 24 51513.0 25 65144.0 26 83361.0 27 106892.0 28 135015.0 29 169222.0 30 210771.0 31 259765.0 32 321468.0 33 402979.0 34 529432.0 35 772608.0 36 1092175.0 37 1865296.0 38 2184731.0 39 590326.0 40 2111.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.551392963452596 29.764599230405242 29.1550812314258 23.528926574716362 2 22.814545160147485 28.77513493331157 27.328568655624828 21.08175125091611 3 21.88614619332713 27.682150359045565 27.845641498166206 22.586061949461097 4 22.207697426554812 27.533267151755815 28.005007254519075 22.2540281671703 5 22.827087390831416 27.617834217023823 28.288622990832767 21.266455401311994 6 22.822389641178447 27.746617478787755 27.16983770814477 22.261155171889033 7 23.051445565754605 27.849707371974624 27.68359628165119 21.41525078061958 8 22.227966844209305 28.008945172850147 27.661609496161983 22.101478486778564 9 21.81484155255919 27.723602630504306 28.28894045471193 22.172615362224573 10-11 21.946894632420168 28.109902989683267 27.642747593287353 22.300454784609215 12-13 21.927836638409854 28.229116360344232 27.4943690554904 22.348677945755515 14-15 22.11799658265186 28.247525269841887 27.33030339955559 22.30417474795067 16-17 22.27592593542078 28.03127649178439 27.353184992249712 22.339612580545115 18-19 22.23700268262811 28.061936189647003 27.328137966349498 22.37292316137539 20-21 22.337038060461296 27.973993419919392 27.38141744130449 22.307551078314827 22-23 22.316440821299253 27.967318602984875 27.36778184271318 22.348458733002687 24-25 22.25947283160161 27.94406091971967 27.33672161690664 22.45974463177208 26-27 22.45324709662142 27.81874187664673 27.417332922325592 22.31067810440626 28-29 22.454276905125774 27.886072468158584 27.355383152406027 22.304267474309615 30-31 22.431438948365273 27.880552522555142 27.322956199231633 22.365052329847952 32-33 22.428089890459624 27.939155581412273 27.245404236198254 22.38735029192985 34-35 22.43805524680102 27.944659163540607 27.26115135374484 22.35613423591353 36-37 22.442757018649015 27.924761177808026 27.21831177590941 22.414170027633546 38-39 22.461809558167626 27.92178847982989 27.22347717955216 22.392924782450326 40-41 22.470994922195494 27.977195206549055 27.204462821475467 22.34734704977998 42-43 22.51276541965714 27.960564461346582 27.150119720638386 22.376550398357892 44-45 22.465120434631352 28.02808015964643 27.152126973585094 22.354672432137125 46-47 22.489758373653846 28.018196620680573 27.123567255028142 22.36847775063744 48-49 22.504130959291274 28.015316649061383 27.110089206030008 22.370463185617336 50-51 22.56769760559814 27.987880555795254 27.092122110360293 22.352299728246315 52-53 22.485449325208467 28.06279799933608 27.0333990526857 22.418353622769754 54-55 22.542039676627187 28.063005270020792 26.94817261982618 22.446782433525836 56-57 22.5224907783636 28.05642169853526 27.00162663851567 22.419460884585465 58-59 22.532821585649103 28.144691737765058 26.93056552060887 22.39192115597697 60-61 22.527141841204152 28.13743386288119 26.926159552059392 22.409264743855264 62-63 22.542258170509324 28.16997369186979 26.898650426519854 22.389117711101036 64-65 22.521835893397324 28.208236295372707 26.9256034089799 22.34432440225007 66-67 22.47657291468941 28.22181058915165 26.93710632133125 22.364510174827693 68-69 22.46270057385243 28.281760686840386 26.92477726107223 22.330761478234958 70-71 22.49642635125431 28.321756076839215 26.854906015506145 22.326911556400322 72-73 22.475892505035862 28.298798211580873 26.84391586771982 22.38139341566345 74-75 22.54787913736308 28.31206817113605 26.812453285669786 22.327599405831084 76-77 22.513622817476527 28.370058069553266 26.79760678816519 22.318712324805016 78-79 22.50921113619831 28.359799142082213 26.782629301506873 22.348360420212604 80-81 22.47374233247963 28.40084234861403 26.762131970204056 22.363283348702286 82-83 22.47622373376931 28.465976799441318 26.73992326335769 22.317876203431688 84-85 22.489411116520678 28.464615304888106 26.737123756765936 22.308849821825277 86-87 22.45478318680194 28.49037405779989 26.722058325454018 22.33278442994415 88-89 22.451649233654795 28.566522226782908 26.705833934824813 22.275994604737487 90-91 22.42918792540232 28.540221345773563 26.70929521145841 22.3212955173657 92-93 22.439773663228074 28.593956056982083 26.679048772006226 22.287221507783617 94-95 22.418565958804784 28.657108008404265 26.66293590450123 22.261390128289726 96-97 22.395614484850938 28.67473024799328 26.683205147134093 22.246450120021684 98-99 22.39661762180436 28.722631908939466 26.6089184710999 22.271831998156273 100-101 22.42701779844419 28.74548922919658 26.54420196507033 22.2832910072889 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 97558.0 1 54889.0 2 9083.5 3 5613.0 4 4896.5 5 4270.0 6 4038.5 7 4134.0 8 3941.0 9 3616.0 10 3522.0 11 4062.5 12 7462.0 13 7468.5 14 4694.5 15 4924.0 16 5477.0 17 6407.5 18 7724.5 19 9163.0 20 11483.0 21 15019.0 22 19541.5 23 25469.0 24 32702.0 25 41061.0 26 53241.5 27 66393.0 28 75624.5 29 84662.0 30 98959.5 31 117418.5 32 134753.5 33 152559.0 34 174012.0 35 195237.5 36 215067.5 37 235122.0 38 249571.0 39 254076.0 40 263141.5 41 277460.5 42 287070.0 43 301410.5 44 317101.5 45 330026.5 46 340855.0 47 348074.5 48 358497.0 49 368258.0 50 370453.5 51 364070.0 52 353313.5 53 339991.5 54 317195.0 55 294261.5 56 270560.5 57 241182.5 58 218123.0 59 191182.0 60 155079.0 61 119735.5 62 88894.5 63 65246.5 64 47625.0 65 32380.0 66 20494.0 67 13666.5 68 8132.0 69 3804.5 70 1717.0 71 761.0 72 387.0 73 185.5 74 80.0 75 55.0 76 36.0 77 26.0 78 20.5 79 13.5 80 11.5 81 9.0 82 6.0 83 5.0 84 4.0 85 4.5 86 3.5 87 2.5 88 2.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012588966850544855 2 0.018261638221674252 3 0.005574490520475235 4 6.218120541430301E-4 5 9.381725729175541E-4 6 0.0 7 0.0 8 0.007592652450588578 9 0.009927174899476445 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.4181678427823492E-4 24-25 0.005061768300392385 26-27 0.007292655406923081 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 6.545390043610843E-5 62-63 9.818085065416265E-5 64-65 2.890880602594789E-4 66-67 1.8545271790230722E-4 68-69 2.999970436654969E-4 70-71 5.9999408733099395E-5 72-73 2.181796681203614E-5 74-75 3.818144192106325E-5 76-77 0.12576966968798237 78-79 0.056252172933132186 80-81 6.545390043610843E-5 82-83 0.0022963410069668042 84-85 0.009758085656683164 86-87 0.11919155269415345 88-89 0.13559320924510163 90-91 0.1489839863759888 92-93 0.14720582208080787 94-95 0.11400978557629486 96-97 1.2142789429238716 98-99 0.07688651504561538 100-101 0.06896113860114324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9166757.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.99234239939247 #Duplication Level Percentage of deduplicated Percentage of total 1 91.00874074276402 65.51932424890607 2 5.2512550010547 7.5610029612490415 3 1.2123866656834335 2.6184766786901856 4 0.5352637797327928 1.5413957321806486 5 0.32675977734453127 1.1762100886468372 6 0.22866956842844902 0.9877474719973325 7 0.16426908347201874 0.8278281272066371 8 0.13099315899468658 0.7544403483458828 9 0.10191291901806268 0.6603254784782934 >10 0.9941321228271337 14.78580321139116 >50 0.04296742885762852 1.9599534736995945 >100 0.002272882745767446 0.240946592986667 >500 1.0767687909504107E-4 0.04957346923517075 >1k 1.0767687909148071E-4 0.29658810522046436 >5k 5.3838439545740356E-5 0.2131775455714263 >10k+ 1.0767687909148071E-4 0.8072064661946406 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10557 0.11516613781733277 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10269 0.11202435059639956 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.090898340601807E-5 2 3.2726950218054216E-5 0.0 0.0 0.0 1.090898340601807E-5 3 7.63628838421265E-5 0.0 0.0 0.0 4.363593362407228E-5 4 7.63628838421265E-5 0.0 0.0 0.0 5.454491703009036E-5 5 8.727186724814457E-5 0.0 0.0 0.0 6.545390043610843E-5 6 8.727186724814457E-5 0.0 0.0 0.0 9.818085065416264E-5 7 8.727186724814457E-5 0.0 0.0 0.0 9.818085065416264E-5 8 9.818085065416264E-5 0.0 0.0 0.0 9.818085065416264E-5 9 1.1999881746619879E-4 0.0 0.0 0.0 1.0908983406018072E-4 10-11 1.52725767684253E-4 0.0 0.0 0.0 1.3636229257522588E-4 12-13 1.9090720960531627E-4 0.0 0.0 0.0 1.52725767684253E-4 14-15 2.9454255196248794E-4 0.0 0.0 0.0 1.9636170130832528E-4 16-17 3.8181441921063253E-4 0.0 0.0 0.0 2.3999763493239758E-4 18-19 4.63631794755768E-4 0.0 0.0 0.0 2.454521266354066E-4 20-21 5.127222200828494E-4 0.0 0.0 0.0 2.727245851504518E-4 22-23 5.290856951918765E-4 0.0 0.0 5.454491703009035E-6 2.781790768534608E-4 24-25 5.563581537069216E-4 0.0 0.0 1.090898340601807E-5 2.8363356855646985E-4 26-27 5.945395956279849E-4 0.0 0.0 1.090898340601807E-5 2.8363356855646985E-4 28-29 6.436300209550662E-4 0.0 0.0 1.090898340601807E-5 2.9454255196248794E-4 30-31 6.654479877671024E-4 0.0 0.0 1.090898340601807E-5 3.2726950218054215E-4 32-33 6.927204462821475E-4 0.0 0.0 1.6363475109027105E-5 3.2726950218054215E-4 34-35 7.690833301242741E-4 0.0 0.0 2.181796681203614E-5 3.4908746899257827E-4 36-37 8.399917222633915E-4 0.0 0.0 2.181796681203614E-5 3.763599275076235E-4 38-39 9.381725729175541E-4 0.0 0.0 2.181796681203614E-5 3.8181441921063253E-4 40-41 0.0010145354567596806 0.0 0.0 2.181796681203614E-5 3.872689109136416E-4 42-43 0.001090898340601807 0.0 0.0 2.181796681203614E-5 4.309048445377138E-4 44-45 0.0011399887659288884 0.0 0.0 2.181796681203614E-5 4.58177303052759E-4 46-47 0.001205442666364997 0.0 0.0 2.181796681203614E-5 4.745407781617861E-4 48-49 0.001238169616583051 0.0 0.0 3.818144192106325E-5 4.7999526986479516E-4 50-51 0.0013636229257522588 0.0 0.0 4.363593362407228E-5 4.7999526986479516E-4 52-53 0.0014890762349214668 0.0 0.0 4.363593362407228E-5 4.7999526986479516E-4 54-55 0.001549075643654566 0.0 0.0 4.363593362407228E-5 4.8544976156780415E-4 56-57 0.001663619969417756 0.0 0.0 4.363593362407228E-5 4.963587449738222E-4 58-59 0.001810891245399 0.0 0.0 4.363593362407228E-5 5.127222200828494E-4 60-61 0.0019472535379742258 0.0 0.0 4.363593362407228E-5 5.236312034888675E-4 62-63 0.002110888289064497 0.0 0.0 4.363593362407228E-5 5.399946785978946E-4 64-65 0.0022254326148276864 0.0 0.0 4.909042532708132E-5 5.563581537069216E-4 66-67 0.0023890673659179578 0.0 0.0 5.454491703009036E-5 5.672671371129397E-4 68-69 0.002607247034038319 1.090898340601807E-5 0.0 5.454491703009036E-5 5.890851039249759E-4 70-71 0.0028145177187526623 1.090898340601807E-5 0.0 5.454491703009036E-5 6.218120541430301E-4 72-73 0.003027242895170015 1.6363475109027105E-5 0.0 5.454491703009036E-5 6.436300209550662E-4 74-75 0.003256331546696394 2.181796681203614E-5 0.0 5.454491703009036E-5 6.490845126580752E-4 76-77 0.0034854201982227736 2.181796681203614E-5 0.0 5.454491703009036E-5 6.545390043610843E-4 78-79 0.003769053766779244 2.181796681203614E-5 0.0 5.454491703009036E-5 6.599934960640933E-4 80-81 0.0042217765781289935 2.181796681203614E-5 0.0 5.9999408733099395E-5 6.763569711731205E-4 82-83 0.004756316765023879 2.181796681203614E-5 0.0 6.545390043610843E-5 6.872659545791386E-4 84-85 0.005416310261087973 2.181796681203614E-5 0.0 6.545390043610843E-5 6.981749379851565E-4 86-87 0.00661629843574996 2.181796681203614E-5 0.0 6.545390043610843E-5 7.090839213911746E-4 88-89 0.007958103394690183 2.181796681203614E-5 0.0 6.545390043610843E-5 7.090839213911746E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 410 0.0 32.38253 3 CTCGCGT 270 0.0 21.947172 52-53 CAACGCA 6945 0.0 20.853134 12-13 ATCAACG 6975 0.0 20.559546 10-11 CGCGTAG 300 0.0 20.542557 54-55 CGTACGC 330 0.0 18.677649 20-21 TCGCGTA 345 0.0 18.550133 52-53 GGTATCA 8855 0.0 18.151587 8 AGTGGTA 8900 0.0 18.00673 5 TCAACGC 6745 0.0 17.851887 10-11 ACTCGCG 350 0.0 17.607906 50-51 GTATCAA 9310 0.0 17.41593 9 CGCTAGT 410 0.0 17.189144 90-91 AACGCAG 7345 0.0 16.329058 12-13 ACGTACG 370 0.0 16.017733 20-21 AAGCAGT 10255 0.0 15.908111 1 TGGTATC 10295 0.0 15.566601 7 ACGCAGA 9455 0.0 15.342363 14-15 CGTAGCG 405 0.0 15.216706 56-57 AGAGTAC 10165 0.0 14.131791 18-19 >>END_MODULE