FastQCFastQC Report
Sat 18 Jun 2016
SRR578559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9051648
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT114983512.703045898382262No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT281420.3109047103908592No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100300.11080855110583178No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG96350.1064447048758414No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT95150.10511897943888228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2537600.091.871245
AAGCAGT2550900.091.666211
GGTATCA2554400.091.381268
GTATCAA2553500.091.362059
TGGTATC2559650.091.161067
GCAGTGG2571950.090.797233
GTGGTAT2575200.090.707046
AGCAGTG2578750.090.6866462
CAGTGGT2572700.090.4802254
TCAACGC2545050.046.4030912-13
CAACGCA2547800.046.23272712-13
AACGCAG2557500.046.2246714-15
ATCAACG2546250.046.22424710-11
CGCAGAG2571450.046.01516-17
ACGCAGA2561950.045.99881414-15
TATCAAC2565000.045.9243210-11
CAGAGTA2553400.045.7955518-19
AGAGTAC2507200.045.62774718-19
GAGTACT1472300.045.4403120-21
GCAGAGT2584550.045.41143816-17
Produced by FastQC (version 0.11.5)