##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578559_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9051648 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.465652994902143 31.0 31.0 34.0 27.0 34.0 2 30.771360419671645 31.0 31.0 34.0 27.0 34.0 3 30.855876742003225 31.0 31.0 34.0 27.0 34.0 4 34.23984980414616 37.0 35.0 37.0 30.0 37.0 5 34.32935483129702 37.0 35.0 37.0 30.0 37.0 6 34.30897456463177 37.0 35.0 37.0 30.0 37.0 7 34.27972331668222 37.0 35.0 37.0 30.0 37.0 8 34.23798870658691 37.0 35.0 37.0 30.0 37.0 9 35.622108592821995 39.0 35.0 39.0 30.0 39.0 10-11 35.58242924382389 39.0 35.0 39.0 30.0 39.0 12-13 35.64189962976908 39.0 35.0 39.0 30.0 39.0 14-15 36.70130914282129 40.0 36.0 41.0 30.0 41.0 16-17 36.657492701881466 40.0 36.0 41.0 30.0 41.0 18-19 36.64791858896855 40.0 36.0 41.0 30.0 41.0 20-21 36.53123204746804 39.5 36.0 41.0 30.0 41.0 22-23 36.230966780855816 39.0 36.0 40.5 28.5 41.0 24-25 36.14297694740229 39.0 36.0 40.5 29.0 41.0 26-27 36.03882066558488 39.0 36.0 41.0 27.0 41.0 28-29 35.77286130658196 39.0 35.0 41.0 27.0 41.0 30-31 35.54221247887678 39.0 34.5 41.0 26.0 41.0 32-33 35.13542208004553 38.0 34.0 40.0 25.0 41.0 34-35 34.931037419926184 38.0 34.0 40.0 25.0 41.0 36-37 34.59526116128245 38.0 33.5 40.0 24.0 41.0 38-39 33.88986397836062 36.5 33.0 40.0 24.0 41.0 40-41 34.05656351197042 37.0 33.0 40.0 24.0 41.0 42-43 33.98206895584097 37.0 33.0 40.0 23.0 41.0 44-45 33.841466051264916 37.0 33.0 40.0 22.5 41.0 46-47 33.63067764013802 37.0 33.0 40.0 22.0 41.0 48-49 33.28228340297811 37.0 33.0 40.0 19.5 41.0 50-51 32.07451063055037 36.0 31.5 39.0 13.0 40.5 52-53 31.67320437118191 36.0 31.0 39.0 5.0 40.0 54-55 31.263634423256406 36.5 30.5 40.0 2.0 41.0 56-57 30.79053637525454 36.5 29.0 40.0 2.0 41.0 58-59 30.460600931454692 36.0 28.5 40.0 2.0 41.0 60-61 30.15506750814879 36.0 28.0 39.5 2.0 41.0 62-63 29.82365669765329 35.0 28.0 39.0 2.0 41.0 64-65 29.492265441607984 35.0 27.5 39.0 2.0 41.0 66-67 29.17608196871995 35.0 26.5 38.5 2.0 40.0 68-69 28.849052901747836 35.0 26.0 38.0 2.0 40.0 70-71 28.4788538507021 35.0 26.0 37.5 2.0 40.0 72-73 28.085312475695034 34.0 26.0 37.0 2.0 39.0 74-75 27.7234504147753 34.0 25.5 36.0 2.0 39.0 76-77 27.331855701856725 34.0 24.5 36.0 2.0 39.0 78-79 26.97524677274238 34.0 24.0 35.5 2.0 37.5 80-81 26.613916880108462 34.0 22.5 35.0 2.0 37.0 82-83 26.237486698554783 33.5 20.5 35.0 2.0 37.0 84-85 25.904615822444708 33.0 20.0 35.0 2.0 36.0 86-87 25.5761794426827 33.0 18.5 35.0 2.0 36.0 88-89 25.217552704214746 33.0 17.5 35.0 2.0 35.5 90-91 24.864788102674783 32.5 10.0 35.0 2.0 35.0 92-93 24.525540266258695 32.0 4.5 35.0 2.0 35.0 94-95 24.095183882537192 32.0 2.0 35.0 2.0 35.0 96-97 23.608885144451044 32.0 2.0 35.0 2.0 35.0 98-99 23.08403210111573 31.5 2.0 35.0 2.0 35.0 100-101 21.781739247924797 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5706135741151037 1101 2 0.628595770200203 1101 3 0.6501938433738914 1101 4 0.6826430209409011 1101 5 0.6945341114622181 1101 6 0.5320008689019247 1101 7 0.46560938277693964 1101 8 0.5209242087923158 1101 9 0.7759354737609456 1101 10-11 0.8203101448949184 1101 12-13 0.7906526275799877 1101 14-15 0.8358107947389044 1101 16-17 0.829676349036518 1101 18-19 0.845632922048857 1101 20-21 0.8858265100178855 1101 22-23 0.8829393490225996 1101 24-25 0.905290710342733 1101 26-27 1.1114803384700664 1101 28-29 1.0663170764589367 1101 30-31 1.1027054159122045 1101 32-33 1.1493959146219765 1101 34-35 1.1978179489019212 1101 36-37 1.1455882211518897 1101 38-39 1.1187991873762968 1101 40-41 1.1482973803917105 1101 42-43 1.2966820927894176 1101 44-45 1.2810104713602684 1101 46-47 1.2546014192959092 1101 48-49 1.2376945862505337 1101 50-51 1.1662163080747305 1101 52-53 1.128698239785873 1101 54-55 1.0952541277768582 1101 56-57 1.086943463925813 1101 58-59 1.0410537649004787 1101 60-61 1.2603885977767106 1101 62-63 1.2675152062421269 1101 64-65 1.0269641572002257 1101 66-67 1.1246049335227717 1101 68-69 1.1151908927610243 1101 70-71 1.1473676807049955 1101 72-73 1.2387669271607145 1101 74-75 1.2019994795266555 1101 76-77 1.2247437126142096 1101 78-79 1.1088726727298663 1101 80-81 1.199646446211137 1101 82-83 1.2500637988793386 1101 84-85 1.1768544838182784 1101 86-87 1.2096715060445469 1101 88-89 1.3194100055072298 1101 90-91 1.3657366133553275 1101 92-93 1.468568646333086 1101 94-95 1.5630866437096032 1101 96-97 1.634149281721463 1101 98-99 1.7189988098428195 1101 100-101 1.933309804528374 1102 1 0.5174012981265825 1102 2 0.4861026353715836 1102 3 0.48397832552375775 1102 4 0.5943557933766783 1102 5 0.496062320200771 1102 6 0.43096529401616124 1102 7 0.42820458492033 1102 8 0.5362341789798251 1102 9 0.563916400806562 1102 10-11 0.6873970962123508 1102 12-13 0.600094356595207 1102 14-15 0.6435929127066302 1102 16-17 0.5958369002933566 1102 18-19 0.680909858166153 1102 20-21 0.769499206267426 1102 22-23 0.6853506931132571 1102 24-25 0.6947877123444854 1102 26-27 0.8696002200625372 1102 28-29 0.7599894308742279 1102 30-31 0.9672852904919296 1102 32-33 0.9325725205675255 1102 34-35 0.9670310572333634 1102 36-37 0.9750798506013609 1102 38-39 0.9767444349788121 1102 40-41 1.1361542423067092 1102 42-43 1.1918120101962444 1102 44-45 1.1770552368367575 1102 46-47 1.2745290672992482 1102 48-49 1.0607218770605797 1102 50-51 1.0969753461579472 1102 52-53 0.9371092832503827 1102 54-55 0.8385233559444423 1102 56-57 0.8779867864196582 1102 58-59 0.7209855634399069 1102 60-61 0.9155667497632862 1102 62-63 0.9929713380773997 1102 64-65 0.8678067165335257 1102 66-67 0.9289265671180864 1102 68-69 0.9678968221474662 1102 70-71 0.9114437035265155 1102 72-73 0.9815274371001941 1102 74-75 1.0610525699185978 1102 76-77 0.9706903566687473 1102 78-79 0.9182719678043583 1102 80-81 0.9633401062628231 1102 82-83 0.9331680886503229 1102 84-85 0.9654602142790303 1102 86-87 0.9775470020551396 1102 88-89 1.0772967322531422 1102 90-91 1.0529703778507802 1102 92-93 1.283562863795641 1102 94-95 1.322665983561972 1102 96-97 1.3938357893453883 1102 98-99 1.4970665687607756 1102 100-101 1.7561643807522742 1103 1 0.39919701305944955 1103 2 0.5113630354333338 1103 3 0.525011668709574 1103 4 0.5799355869197456 1103 5 0.5012392489785071 1103 6 0.46841471424578174 1103 7 0.38460267107269885 1103 8 0.43360615512411727 1103 9 0.4902424770602636 1103 10-11 0.5983709393810841 1103 12-13 0.590837975777248 1103 14-15 0.7193841026562282 1103 16-17 0.6732355107428774 1103 18-19 0.6538508347405099 1103 20-21 0.7352718527629065 1103 22-23 0.5758175480189536 1103 24-25 0.5958399176762796 1103 26-27 0.7695421642099305 1103 28-29 0.805403071224525 1103 30-31 0.8445693214086702 1103 32-33 0.7996254148130859 1103 34-35 0.8631567030226037 1103 36-37 0.879221172990718 1103 38-39 0.9222988832026999 1103 40-41 0.9046630019081903 1103 42-43 0.964493138903805 1103 44-45 0.9305002898110715 1103 46-47 0.914153533793737 1103 48-49 1.1641171209472532 1103 50-51 1.1994493253958538 1103 52-53 1.1568173024178883 1103 54-55 0.9721201824892596 1103 56-57 0.9610880262707617 1103 58-59 0.9659192452554386 1103 60-61 1.0456419821270444 1103 62-63 1.1044368284180592 1103 64-65 1.097658158563977 1103 66-67 1.15469043046566 1103 68-69 1.1185146627193099 1103 70-71 1.1008836314943053 1103 72-73 1.1024998736120573 1103 74-75 1.0434997756074509 1103 76-77 1.054633329161252 1103 78-79 1.061234995159424 1103 80-81 1.0653153633693258 1103 82-83 1.0697367781596974 1103 84-85 1.156767927874963 1103 86-87 1.233775037903463 1103 88-89 1.2284586901616414 1103 90-91 1.2260884465937423 1103 92-93 1.3236794101475482 1103 94-95 1.4260464389201921 1103 96-97 1.4145931162001588 1103 98-99 1.4839308314954494 1103 100-101 1.8362233389931717 1104 1 0.3760521447224008 1104 2 0.26964374128342783 1104 3 0.3421249250413858 1104 4 0.4755388178175224 1104 5 0.36254618511534176 1104 6 0.34319863762389957 1104 7 0.31572837133307274 1104 8 0.3828330793045467 1104 9 0.4051280153902965 1104 10-11 0.5478045189335887 1104 12-13 0.5072669020990119 1104 14-15 0.5088487271813946 1104 16-17 0.5940428424570499 1104 18-19 0.5949744416591827 1104 20-21 0.5778391806079739 1104 22-23 0.5281252602801061 1104 24-25 0.5722379323592506 1104 26-27 0.5392009882997684 1104 28-29 0.5406673092511696 1104 30-31 0.6488511809603494 1104 32-33 0.5885908373414424 1104 34-35 0.6607233599559024 1104 36-37 0.7765925079306015 1104 38-39 0.7428445169003837 1104 40-41 0.8428525728482938 1104 42-43 0.8453850079258274 1104 44-45 0.845046275267741 1104 46-47 0.7343490388181806 1104 48-49 0.832189023537893 1104 50-51 0.6770925511202748 1104 52-53 0.7870199989862563 1104 54-55 0.8034542290375661 1104 56-57 0.6486332188757764 1104 58-59 0.58351064289376 1104 60-61 0.44186636096395304 1104 62-63 0.5360811549953652 1104 64-65 0.6697199559020781 1104 66-67 0.44716797007278686 1104 68-69 0.46183264811246616 1104 70-71 0.5250016006934572 1104 72-73 0.4345841413430662 1104 74-75 0.5876011701948478 1104 76-77 0.6108311054675077 1104 78-79 0.5570291116692232 1104 80-81 0.47144570304220323 1104 82-83 0.4089590986853402 1104 84-85 0.595538592048527 1104 86-87 0.647658190405771 1104 88-89 0.600044061351614 1104 90-91 0.5546142091816435 1104 92-93 0.7736367190676923 1104 94-95 0.753529421579394 1104 96-97 0.6404349953252542 1104 98-99 0.8597629469451213 1104 100-101 1.044550917758034 1105 1 -0.04525136459845669 1105 2 9.520126018713881E-5 1105 3 0.13664991385656577 1105 4 0.1526575334475453 1105 5 0.16136541073355914 1105 6 0.14516720100807845 1105 7 0.14082483002977852 1105 8 0.1626280571875327 1105 9 0.13448595336685543 1105 10-11 0.09982436902522807 1105 12-13 0.07002976286520379 1105 14-15 -0.04039916393285381 1105 16-17 0.08220898469836158 1105 18-19 -0.010464175515288332 1105 20-21 0.23090851856975547 1105 22-23 0.22382452558731103 1105 24-25 0.27024475275414517 1105 26-27 0.428629492776345 1105 28-29 0.4527804247492071 1105 30-31 0.40246892916240284 1105 32-33 0.4486321079743547 1105 34-35 0.38471680332288116 1105 36-37 0.40735773730873603 1105 38-39 0.4631614192753304 1105 40-41 0.3073478992337968 1105 42-43 0.5076014981480768 1105 44-45 0.437982084217424 1105 46-47 0.5231401673279663 1105 48-49 0.5604467259994621 1105 50-51 0.5544568520169761 1105 52-53 0.4349271685596712 1105 54-55 0.5727108593764676 1105 56-57 0.5140672018601613 1105 58-59 0.495752043635985 1105 60-61 0.3677772004755724 1105 62-63 0.36095648729832064 1105 64-65 0.4024902650565174 1105 66-67 0.5298732682772673 1105 68-69 0.41712082602666456 1105 70-71 0.22300663781097896 1105 72-73 0.381352437725166 1105 74-75 0.2605966571627327 1105 76-77 0.235061153609589 1105 78-79 0.2925569212678809 1105 80-81 0.3089187127604198 1105 82-83 0.23945245402486037 1105 84-85 0.3233139879142506 1105 86-87 0.4367229587484225 1105 88-89 0.5305242577359088 1105 90-91 0.5651352134791665 1105 92-93 0.7143022323540684 1105 94-95 0.8168671911551328 1105 96-97 0.8402175811788375 1105 98-99 0.9038012554727395 1105 100-101 1.0667130984428468 1106 1 0.11310549938473358 1106 2 0.19258635846546213 1106 3 0.2110838284793708 1106 4 0.3716845809203946 1106 5 0.24703290067380834 1106 6 0.25867217619907734 1106 7 0.21831843813885143 1106 8 0.1757456914435238 1106 9 0.0343417046212906 1106 10-11 0.20837870404579917 1106 12-13 0.23070498591737731 1106 14-15 0.2401678410127701 1106 16-17 0.22899860989610232 1106 18-19 0.1632595810430857 1106 20-21 0.22210429636679407 1106 22-23 0.22801253830139245 1106 24-25 0.24649309767696082 1106 26-27 0.119079867634035 1106 28-29 0.2232276079984743 1106 30-31 0.3017738268052881 1106 32-33 0.3483593738591608 1106 34-35 0.32242921341103425 1106 36-37 0.3646976345243331 1106 38-39 0.5330515802870082 1106 40-41 0.2886067727886328 1106 42-43 0.27450627316962795 1106 44-45 0.43038146550434675 1106 46-47 0.3669136492874401 1106 48-49 0.24800440800636636 1106 50-51 0.37590541557505475 1106 52-53 0.38805544693049043 1106 54-55 0.14474857710666456 1106 56-57 0.2502244276910446 1106 58-59 0.5023409137787844 1106 60-61 0.41212030071618244 1106 62-63 0.397134660349618 1106 64-65 0.4273070988305072 1106 66-67 0.549025999072601 1106 68-69 0.5394982195454681 1106 70-71 0.4757221415446793 1106 72-73 0.5749563981227901 1106 74-75 0.6478488988548392 1106 76-77 0.5709509265028068 1106 78-79 0.5575191573560083 1106 80-81 0.5413894258999683 1106 82-83 0.4623589569487656 1106 84-85 0.5569650133140662 1106 86-87 0.6508761790145563 1106 88-89 0.6285284902126556 1106 90-91 0.6322293791761595 1106 92-93 0.6624031549237976 1106 94-95 0.6062455007154739 1106 96-97 0.7504836074427992 1106 98-99 0.8043568715338338 1106 100-101 0.9810755813607486 1107 1 0.10505604913927158 1107 2 0.12353888699628612 1107 3 0.27442241432003556 1107 4 0.4657481193764923 1107 5 0.2322734367822079 1107 6 0.2667901540329325 1107 7 0.3013510589801811 1107 8 0.27239580338616065 1107 9 0.2659448021852313 1107 10-11 0.32577626868280873 1107 12-13 0.3605588959841697 1107 14-15 0.4518090848512557 1107 16-17 0.44041429406411936 1107 18-19 0.44732626565092204 1107 20-21 0.4619439849911018 1107 22-23 0.4521451728977226 1107 24-25 0.6330526245590704 1107 26-27 0.373534807472339 1107 28-29 0.3269752900477343 1107 30-31 0.4346040539894318 1107 32-33 0.6019855785570698 1107 34-35 0.40081013342325633 1107 36-37 0.45923549094538174 1107 38-39 0.5462463740164907 1107 40-41 0.40862726629342916 1107 42-43 0.399850221164769 1107 44-45 0.6048049195359013 1107 46-47 0.48520054324109907 1107 48-49 0.6611488327214232 1107 50-51 0.41460151324038463 1107 52-53 0.6403277538412446 1107 54-55 0.5521537544458077 1107 56-57 0.551862849911128 1107 58-59 0.7396674706099908 1107 60-61 0.6958546703761712 1107 62-63 0.6621718667593903 1107 64-65 0.6554293288833115 1107 66-67 0.7924372468720442 1107 68-69 0.7609847491401673 1107 70-71 0.6872816166993694 1107 72-73 0.6627941140881504 1107 74-75 0.7557412967770709 1107 76-77 0.8192301800914059 1107 78-79 0.7435538544460769 1107 80-81 0.6054263769112218 1107 82-83 0.5708751988193477 1107 84-85 0.6184344625141875 1107 86-87 0.7462103959572026 1107 88-89 0.7990509657323166 1107 90-91 0.7376677213941605 1107 92-93 0.7800485894930596 1107 94-95 0.7830155636007277 1107 96-97 0.7970245171761121 1107 98-99 0.8607090000182716 1107 100-101 0.9819990507755847 1108 1 0.1631020847112552 1108 2 0.10908133052497604 1108 3 0.11709241784908997 1108 4 0.33718307104180667 1108 5 0.17093384501794873 1108 6 0.32570954074864034 1108 7 0.24672841249054756 1108 8 0.15469536839884768 1108 9 0.23175033256929822 1108 10-11 0.284753146087823 1108 12-13 0.14235257121135447 1108 14-15 0.35088369128619945 1108 16-17 0.21116411799700785 1108 18-19 0.24409981763712807 1108 20-21 0.27562845301819294 1108 22-23 0.22541300503809936 1108 24-25 0.42704692785179077 1108 26-27 0.3425499418738056 1108 28-29 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98-99 0.11830315717143591 2312 100-101 0.028894327519029872 2313 1 -0.5624994119785853 2313 2 -0.39447765169550664 2313 3 -0.14934681663642024 2313 4 0.047790621962946034 2313 5 -0.2905898937896154 2313 6 -0.19487559809785182 2313 7 -0.23134779865008426 2313 8 -0.0852389395158113 2313 9 0.05015011568625738 2313 10-11 -0.17262043297877483 2313 12-13 -0.1819434806481155 2313 14-15 -0.44766600505456466 2313 16-17 -0.22843092072410087 2313 18-19 -0.21815911559513523 2313 20-21 -0.13825695762150048 2313 22-23 -0.11635438635417472 2313 24-25 -0.5403139721144115 2313 26-27 -0.4035981156179318 2313 28-29 -0.4132598030943413 2313 30-31 -0.5345007858972721 2313 32-33 -0.9120836145730138 2313 34-35 -0.39557135527385157 2313 36-37 -0.16977933276083235 2313 38-39 -0.24806851782250305 2313 40-41 -0.11326054768242244 2313 42-43 -0.27935758353032014 2313 44-45 -0.3120873369609498 2313 46-47 -0.34880334715928996 2313 48-49 -0.30169024915113596 2313 50-51 -0.3193159491130686 2313 52-53 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9 -0.5052118990503658 2314 10-11 -0.5659020343824608 2314 12-13 -0.5709445669929494 2314 14-15 -0.7228386314147954 2314 16-17 -0.7527170295433905 2314 18-19 -2.395992474879158 2314 20-21 -1.0957386833913816 2314 22-23 -0.7875886684513702 2314 24-25 -0.9326383698375835 2314 26-27 -1.0563797584541206 2314 28-29 -0.7604403119734187 2314 30-31 -0.8054875851144772 2314 32-33 -0.8077252404700133 2314 34-35 -0.8673009864425936 2314 36-37 -0.947283877417398 2314 38-39 -0.6518749391835001 2314 40-41 -0.5896110895251994 2314 42-43 -0.9977530635679841 2314 44-45 -0.9120214292731177 2314 46-47 -0.7878581752807321 2314 48-49 -0.7821383718468411 2314 50-51 -0.8304974116713453 2314 52-53 -0.7182822098528554 2314 54-55 -0.5789804390538009 2314 56-57 -0.5544037807827671 2314 58-59 -0.7534279095950147 2314 60-61 -0.6802821965372452 2314 62-63 -0.8132842267822333 2314 64-65 -0.7685964998099379 2314 66-67 -0.8266048782334856 2314 68-69 -0.7791843736520114 2314 70-71 -0.9336131281628894 2314 72-73 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-1.3588251544322034 2315 30-31 -1.5686269108639692 2315 32-33 -1.8836904876582068 2315 34-35 -1.9382792691867152 2315 36-37 -1.758587443320998 2315 38-39 -1.9055310777982442 2315 40-41 -2.8052462603217165 2315 42-43 -2.3866164440876823 2315 44-45 -1.961500362812437 2315 46-47 -1.6708977664373847 2315 48-49 -2.084979905489625 2315 50-51 -1.8854263447456603 2315 52-53 -1.6277456215359116 2315 54-55 -1.5169797530025377 2315 56-57 -1.5758201580276143 2315 58-59 -1.7800215431736284 2315 60-61 -1.5133302604811192 2315 62-63 -1.2960161974194193 2315 64-65 -1.325261780020515 2315 66-67 -1.3869711468358545 2315 68-69 -1.6323278991965928 2315 70-71 -1.3678650292365475 2315 72-73 -1.4166435726196305 2315 74-75 -1.5100218622998298 2315 76-77 -1.4258814140717035 2315 78-79 -1.5085660665408156 2315 80-81 -1.5122304077094206 2315 82-83 -1.438900520996068 2315 84-85 -1.541386148277617 2315 86-87 -1.603244641939643 2315 88-89 -1.6499172905137378 2315 90-91 -1.6265949435388407 2315 92-93 -1.7176062547393052 2315 94-95 -1.6297221807571702 2315 96-97 -1.7553352382975298 2315 98-99 -1.7949708159505597 2315 100-101 -2.0742116891175506 2316 1 -0.9646429258902103 2316 2 -1.0774342167491682 2316 3 -1.4468770295350275 2316 4 -1.2758068354644791 2316 5 -0.9111625770933074 2316 6 -0.7939095577671509 2316 7 -0.9790628537757158 2316 8 -1.111067239604175 2316 9 -1.4092688321810343 2316 10-11 -1.4551067498886923 2316 12-13 -1.2314011805899199 2316 14-15 -1.2774578207930176 2316 16-17 -2.0310176190430056 2316 18-19 -1.4128111480626728 2316 20-21 -1.701380555726395 2316 22-23 -1.3107353329723352 2316 24-25 -1.2684127464838681 2316 26-27 -1.3518882849378429 2316 28-29 -1.7530540871486622 2316 30-31 -1.5758048728132508 2316 32-33 -1.6454508220817345 2316 34-35 -2.0869153021763935 2316 36-37 -1.8299511752184614 2316 38-39 -1.7608641390109838 2316 40-41 -2.1544927790893524 2316 42-43 -2.3207647239997407 2316 44-45 -2.5966375344133326 2316 46-47 -2.311913304110007 2316 48-49 -2.971450319796869 2316 50-51 -2.3663479083134504 2316 52-53 -3.0827768714080612 2316 54-55 -4.196228976942436 2316 56-57 -2.890431459533044 2316 58-59 -2.3499760884777707 2316 60-61 -2.0979151266951845 2316 62-63 -2.676216101921735 2316 64-65 -2.670951571831136 2316 66-67 -2.422085850569278 2316 68-69 -2.3689248246286105 2316 70-71 -2.6323194727201127 2316 72-73 -5.599467405414547 2316 74-75 -3.309279257680391 2316 76-77 -2.7607426674048945 2316 78-79 -2.672940580107465 2316 80-81 -2.5279365972680914 2316 82-83 -2.6961287166484986 2316 84-85 -2.814363438415146 2316 86-87 -2.618718742116773 2316 88-89 -2.406495960446641 2316 90-91 -2.415531513142973 2316 92-93 -2.765048843618654 2316 94-95 -2.958542693196023 2316 96-97 -3.0764828413192227 2316 98-99 -3.3923744129892235 2316 100-101 -3.457363507531092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 138714.0 3 21124.0 4 17043.0 5 16182.0 6 17414.0 7 20580.0 8 25015.0 9 28829.0 10 33126.0 11 39479.0 12 40765.0 13 45776.0 14 51887.0 15 62365.0 16 78668.0 17 104760.0 18 151304.0 19 220968.0 20 289903.0 21 212504.0 22 128222.0 23 114164.0 24 117987.0 25 126702.0 26 139743.0 27 158064.0 28 181583.0 29 212489.0 30 250580.0 31 298950.0 32 362760.0 33 448689.0 34 573983.0 35 795759.0 36 1141160.0 37 1268511.0 38 955503.0 39 160183.0 40 210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.59404206227595 47.898423372413575 24.27945438652988 17.228080178780598 2 16.344057202519576 49.52889785815621 18.219764076868124 15.907280862456085 3 44.40534758364888 20.426065356898487 19.065628028372657 16.102959031079976 4 16.383766214933644 18.868212962825616 20.05393742302521 44.69408339921552 5 16.406707530576345 47.209070739834594 20.895841033413713 15.488380696175346 6 44.79650012639194 20.00266938013101 18.787449003671007 16.413381489806042 7 17.19525165927185 19.481009357477124 48.00229397415984 15.321445009091189 8 44.31385150013938 20.223616352121283 19.640398669009784 15.822133478729555 9 43.90422721209741 19.852532736105065 20.251816362922316 15.991423688875209 10-11 16.05657063169385 33.72426513228299 33.87476951683919 16.34439471918397 12-13 15.905878263684405 20.154347787152155 33.81779352679238 30.12198042237106 14-15 16.046637630414505 47.83175904528529 19.733195366265694 16.388407958034513 16-17 30.053856268457956 19.940958866219198 19.8438227912529 30.16136207406995 18-19 16.213332711349945 33.7707464668405 19.8529359338503 30.162984887959254 20-21 30.183139190261315 33.8129113502194 19.86090120741488 16.143048252104403 22-23 30.13672520449926 19.92027816983524 33.76069193199163 16.182304693673867 24-25 16.15596025613528 33.60931427956577 20.304102206530445 29.930623257768502 26-27 16.344341635059745 25.36277592412393 41.85558793739243 16.437294503423892 28-29 27.506081946541205 19.753613053690344 36.423070491338166 16.317234508430285 30-31 25.44185280616939 20.924966389623968 36.81523383041141 16.817946973795234 32-33 19.691949883488334 23.51324189119525 38.10283231465406 18.69197591066236 34-35 19.264318706174148 22.65435426370359 38.71462664704623 19.366700383076036 36-37 18.98929638426909 22.50843058952783 39.02521274044174 19.47706028576134 38-39 19.099269370017684 22.65764258879588 38.86602663153774 19.377061409648697 40-41 19.20983128013003 22.388749282240063 38.72013524559134 19.68128419203857 42-43 19.69287929597609 22.64802009854743 38.741663369225705 18.917437236250777 44-45 19.288118573031692 22.794784948628465 38.559469309581296 19.357627168758547 46-47 19.565740947055506 22.531860157494794 38.647644298019905 19.25475459742979 48-49 19.41466757765606 22.626855678546 38.77259357221974 19.18588317157819 50-51 19.59818264772757 22.590893034536634 38.03354632375989 19.777377993975907 52-53 19.984073881275812 23.741786886206167 36.848236577464924 19.42590265505309 54-55 20.46180593164702 24.34997026881851 35.39378894618987 19.79443485334459 56-57 20.490522199418713 25.133432591051974 34.24207270983409 20.13397249969523 58-59 20.692341085545504 25.809779321287436 33.28994238826864 20.20793720489842 60-61 21.015994525649305 26.033097450361613 32.79793502646043 20.15297299752865 62-63 21.107023563496412 25.81762266847805 32.827805822212106 20.247547945813427 64-65 20.465546837091214 25.498081729063436 33.057323749531626 20.979047684313727 66-67 20.707914356707935 25.242508019035352 33.76151074133228 20.288066882924433 68-69 20.582346572970714 25.005788760796243 33.81975318602895 20.592111480204096 70-71 20.363197279430402 25.02184442919802 34.438034166068945 20.17692412530263 72-73 19.988820783285664 24.710252165109793 34.43110331077228 20.86982374083227 74-75 20.35146598718838 25.057996369869638 33.61100814952825 20.97952949341372 76-77 20.80827041387604 25.576056845445187 33.096051778840724 20.519620961838044 78-79 20.76137335322579 25.622082771654362 32.913555845244026 20.702988029875822 80-81 20.454252185103513 26.07958158571749 32.774737312594986 20.691428916584005 82-83 21.383851887408106 26.322854048003265 32.01812253968215 20.275171524906472 84-85 20.8828189842975 26.72908475011629 31.513865100610943 20.87423116497527 86-87 20.828081756699774 27.09424475834347 31.33851345204964 20.739160032907115 88-89 20.80552827325403 26.67035518839561 31.377696575283835 21.14641996306652 90-91 20.72938459228474 26.494223346047658 31.556549623806397 21.219842437861203 92-93 20.670434930419045 26.997635505307127 31.384981159418047 20.94694840485578 94-95 20.79242665036202 26.390031441045913 31.057629170287743 21.759912738304326 96-97 20.730252430094197 26.276252888377865 31.39716126032098 21.596333421206953 98-99 21.11502487120123 26.701394288083762 30.947297190215707 21.236283650499306 100-101 21.382940152613415 26.273429589428073 30.834980127413957 21.508650130544552 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12461.0 1 7631.5 2 2099.0 3 1259.5 4 1061.0 5 955.0 6 1045.5 7 1241.5 8 1170.5 9 1202.5 10 1653.5 11 10591.0 12 188121.5 13 213200.0 14 62120.5 15 55356.0 16 57006.5 17 59689.0 18 62099.0 19 62598.0 20 62859.0 21 63540.0 22 64642.0 23 67088.5 24 72001.0 25 78634.5 26 85549.5 27 93110.0 28 100263.5 29 108392.5 30 117224.0 31 126089.5 32 135660.0 33 147039.0 34 166050.0 35 181614.0 36 191163.0 37 201011.5 38 210100.5 39 221451.5 40 233028.0 41 242536.5 42 249291.0 43 258172.0 44 269207.5 45 276659.5 46 279674.5 47 284975.0 48 292771.5 49 295969.5 50 293387.0 51 292675.0 52 295596.0 53 291991.5 54 270215.5 55 241809.0 56 226195.5 57 213778.5 58 216807.5 59 188501.0 60 134652.0 61 105053.0 62 77315.0 63 56843.0 64 42042.0 65 30024.0 66 20316.5 67 13565.0 68 9254.0 69 6348.5 70 4945.5 71 4286.5 72 3919.5 73 3649.0 74 3430.5 75 3227.0 76 3121.5 77 2971.0 78 2757.5 79 2600.5 80 2501.0 81 2336.5 82 2121.5 83 1927.0 84 1783.5 85 1522.5 86 1308.0 87 1154.5 88 901.0 89 711.5 90 583.5 91 460.5 92 336.5 93 241.5 94 159.5 95 100.0 96 59.5 97 29.0 98 19.0 99 10.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10004807964251372 2 0.08209554768369251 3 0.08851426834096951 4 0.19715746789976807 5 0.07544482507494768 6 0.09224839498840433 7 0.0975071058883421 8 0.08677977756094801 9 0.07954352621754623 10-11 0.07800789425306862 12-13 0.07099259714916002 14-15 0.07708541030318457 16-17 0.06728056592567452 18-19 0.058757256137224954 20-21 0.05347644981333786 22-23 0.04350588975620793 24-25 0.04599714880649358 26-27 0.04476532892131908 28-29 0.043848368827422365 30-31 0.034281050257367496 32-33 0.03770031711352452 34-35 0.051029381610950844 36-37 0.04682020334860569 38-39 0.050736617243622384 40-41 0.04864307582442445 42-43 0.0467097262288591 44-45 0.05043832902030657 46-47 0.04401408450704226 48-49 0.039882240228519716 50-51 0.04396989365914362 52-53 0.03997062192431698 54-55 0.04317445839696815 56-57 0.04221330745517281 58-59 0.04911260358334748 60-61 0.048223262769387404 62-63 0.052968255062503536 64-65 0.04902974574353753 66-67 0.04372132013971378 68-69 0.04356112831608123 70-71 0.05213415280841677 72-73 0.0398601448045704 74-75 0.04766535331466712 76-77 0.046052387366366875 78-79 0.04416322861870015 80-81 0.0437986541235364 82-83 0.04319102996493014 84-85 0.04363293844391651 86-87 0.048223262769387404 88-89 0.041533873168731264 90-91 0.04396436980315629 92-93 0.04005900362011426 94-95 0.043318078652638724 96-97 0.029469771692403415 98-99 0.035695157390123876 100-101 0.024824208807059225 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9051648.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.666922837613726 #Duplication Level Percentage of deduplicated Percentage of total 1 91.73041413408481 47.39428228928112 2 4.743420920681786 4.90155925390377 3 1.2557474967080393 1.946418270478226 4 0.597812437497837 1.235485163182661 5 0.3384617928798875 0.8743639668102775 6 0.22861468143861116 0.708709026326062 7 0.18008958223142427 0.6513272184306365 8 0.13721411194982763 0.5671544747474748 9 0.11689295569975275 0.5435549390159753 >10 0.6403764611792183 5.321261146266776 >50 0.014670798884764408 0.5480560297873017 >100 0.009724146883126854 1.0820948447448309 >500 0.0029312782451889805 1.037842044209222 >1k 0.00316391937488355 3.389237481977789 >5k 3.256975827086007E-4 1.2393187921296658 >10k+ 1.39584678303686E-4 28.559335058708278 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1149835 12.703045898382262 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 28142 0.3109047103908592 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10030 0.11080855110583178 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9635 0.1064447048758414 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 9515 0.10511897943888228 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.20954239493184E-5 0.0 4 1.10477119746592E-5 0.0 0.0 2.20954239493184E-5 0.0 5 1.10477119746592E-5 0.0 0.0 3.3143135923977603E-5 0.0 6 1.10477119746592E-5 0.0 0.0 5.5238559873296E-5 0.0 7 1.10477119746592E-5 0.0 0.0 5.5238559873296E-5 0.0 8 1.10477119746592E-5 0.0 0.0 5.5238559873296E-5 0.0 9 1.10477119746592E-5 0.0 0.0 5.5238559873296E-5 0.0 10-11 1.10477119746592E-5 0.0 0.0 6.628627184795521E-5 5.5238559873296E-6 12-13 2.7619279936648E-5 0.0 0.0 7.73339838226144E-5 1.10477119746592E-5 14-15 3.3143135923977603E-5 0.0 0.0 8.285783980994401E-5 1.10477119746592E-5 16-17 3.3143135923977603E-5 0.0 0.0 1.1600097573392161E-4 1.10477119746592E-5 18-19 4.41908478986368E-5 0.0 0.0 1.4914411165789922E-4 1.10477119746592E-5 20-21 4.9714703885966405E-5 0.0 0.0 1.878111035692064E-4 1.10477119746592E-5 22-23 6.0762415860625604E-5 0.0 0.0 1.878111035692064E-4 1.10477119746592E-5 24-25 7.18101278352848E-5 0.0 0.0 1.9333495955653598E-4 1.10477119746592E-5 26-27 7.73339838226144E-5 0.0 0.0 1.988588155438656E-4 1.10477119746592E-5 28-29 7.73339838226144E-5 0.0 0.0 1.988588155438656E-4 1.10477119746592E-5 30-31 7.73339838226144E-5 0.0 0.0 2.20954239493184E-4 1.10477119746592E-5 32-33 7.73339838226144E-5 0.0 0.0 2.320019514678432E-4 1.10477119746592E-5 34-35 7.73339838226144E-5 0.0 0.0 2.48573519429832E-4 1.10477119746592E-5 36-37 7.73339838226144E-5 0.0 0.0 2.6514508739182083E-4 1.10477119746592E-5 38-39 8.838169579727359E-5 0.0 0.0 2.6514508739182083E-4 1.10477119746592E-5 40-41 9.94294077719328E-5 0.0 0.0 2.706689433791504E-4 2.20954239493184E-5 42-43 9.94294077719328E-5 0.0 0.0 2.8171665535380963E-4 2.20954239493184E-5 44-45 1.1600097573392161E-4 0.0 0.0 2.9828822331579843E-4 2.20954239493184E-5 46-47 1.3809639968324E-4 0.0 0.0 3.093359352904576E-4 2.20954239493184E-5 48-49 1.7676339159454718E-4 1.10477119746592E-5 0.0 3.259075032524464E-4 2.20954239493184E-5 50-51 1.9333495955653598E-4 1.10477119746592E-5 0.0 3.535267831890944E-4 2.20954239493184E-5 52-53 2.20954239493184E-4 1.6571567961988802E-5 0.0 3.756222071384128E-4 2.20954239493184E-5 54-55 2.9828822331579843E-4 2.20954239493184E-5 0.0 3.86669919113072E-4 2.20954239493184E-5 56-57 4.087653430623904E-4 2.20954239493184E-5 0.0 3.921937751004016E-4 2.20954239493184E-5 58-59 5.358140307709712E-4 2.7619279936648E-5 0.0 4.0324148707506077E-4 2.20954239493184E-5 60-61 6.573388624922223E-4 3.86669919113072E-5 0.0 4.1981305503704957E-4 2.20954239493184E-5 62-63 7.346728463148368E-4 4.41908478986368E-5 0.0 4.41908478986368E-4 2.20954239493184E-5 64-65 8.175306861247808E-4 4.41908478986368E-5 0.0 4.584800469483568E-4 2.20954239493184E-5 66-67 9.445793738333617E-4 4.41908478986368E-5 0.0 4.750516149103456E-4 2.20954239493184E-5 68-69 0.0010881996295039314 4.41908478986368E-5 0.0 5.081947508343232E-4 2.20954239493184E-5 70-71 0.001298106157022456 4.41908478986368E-5 0.0 5.413378867583008E-4 4.41908478986368E-5 72-73 0.0015301081084902993 4.41908478986368E-5 0.0 5.413378867583008E-4 6.628627184795521E-5 74-75 0.0017234430680468354 4.41908478986368E-5 0.0 5.468617427456305E-4 8.83816957972736E-5 76-77 0.0020438267153119524 5.5238559873296E-5 0.0 5.634333107076193E-4 8.83816957972736E-5 78-79 0.0023531626506024094 5.5238559873296E-5 0.0 5.800048786696081E-4 9.942940777193281E-5 80-81 0.0029331675292720177 5.5238559873296E-5 0.0 6.07624158606256E-4 1.10477119746592E-4 82-83 0.0036844119435488435 5.5238559873296E-5 0.0 6.35243438542904E-4 1.10477119746592E-4 84-85 0.0046400390293568645 5.5238559873296E-5 0.0 6.407672945302336E-4 1.10477119746592E-4 86-87 0.006015479170201935 5.5238559873296E-5 0.0 6.573388624922223E-4 1.10477119746592E-4 88-89 0.007799684654109395 6.628627184795521E-5 0.0 6.739104304542112E-4 1.10477119746592E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 253760 0.0 91.87124 5 AAGCAGT 255090 0.0 91.66621 1 GGTATCA 255440 0.0 91.38126 8 GTATCAA 255350 0.0 91.36205 9 TGGTATC 255965 0.0 91.16106 7 GCAGTGG 257195 0.0 90.79723 3 GTGGTAT 257520 0.0 90.70704 6 AGCAGTG 257875 0.0 90.686646 2 CAGTGGT 257270 0.0 90.480225 4 TCAACGC 254505 0.0 46.40309 12-13 CAACGCA 254780 0.0 46.232727 12-13 AACGCAG 255750 0.0 46.22467 14-15 ATCAACG 254625 0.0 46.224247 10-11 CGCAGAG 257145 0.0 46.015 16-17 ACGCAGA 256195 0.0 45.998814 14-15 TATCAAC 256500 0.0 45.92432 10-11 CAGAGTA 255340 0.0 45.79555 18-19 AGAGTAC 250720 0.0 45.627747 18-19 GAGTACT 147230 0.0 45.44031 20-21 GCAGAGT 258455 0.0 45.411438 16-17 >>END_MODULE