FastQCFastQC Report
Sat 18 Jun 2016
SRR578559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9051648
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1384691.529765629419085No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151780.16768217235137733No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102660.11341581113185135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3750.039.266573
CGCGTAG2600.027.40340254-55
CGTACGC2900.026.2055320-21
CTCGCGT2900.024.56972152-53
ACGTACG3400.022.35177620-21
TCGCGTA3550.021.4091152-53
ACTCGCG3450.021.34182750-51
CAACGCA60250.019.74793412-13
ATCAACG61800.019.02206810-11
CGCTAGT4100.018.53633590-91
AGTGGTA78550.017.838425
TCAACGC59900.017.40518610-11
GGTATCA84700.016.9354578
CGTAGCG4250.016.7641156-57
GTATCAA83850.016.6539659
AACGCAG64650.016.31005712-13
AAGCAGT92250.015.4169191
TGGTATC96650.014.98895557
GTCACGT11050.014.615354
ACGTGTA10750.014.5807177
Produced by FastQC (version 0.11.5)