##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578559_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9051648 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.483814991480006 31.0 31.0 34.0 30.0 34.0 2 31.80114284161293 31.0 31.0 34.0 30.0 34.0 3 31.872269889416824 31.0 31.0 34.0 30.0 34.0 4 35.544308506031165 37.0 35.0 37.0 33.0 37.0 5 35.36351015859211 37.0 35.0 37.0 33.0 37.0 6 35.36113224906669 37.0 35.0 37.0 32.0 37.0 7 35.33664057639007 37.0 35.0 37.0 32.0 37.0 8 35.3240000053029 37.0 35.0 37.0 32.0 37.0 9 36.89135569567 39.0 37.0 39.0 33.0 39.0 10-11 36.85835916288393 39.0 37.0 39.0 32.5 39.0 12-13 36.836627595328494 39.0 37.0 39.0 32.0 39.0 14-15 38.01695497880607 40.0 37.0 41.0 33.0 41.0 16-17 37.96763346298928 40.0 37.0 41.0 33.0 41.0 18-19 37.89940108143843 40.0 37.0 41.0 33.0 41.0 20-21 37.84702106180002 40.0 37.0 41.0 32.0 41.0 22-23 37.75775576999901 40.0 37.0 41.0 32.0 41.0 24-25 37.671358022318145 39.5 36.5 41.0 32.0 41.0 26-27 37.487416932253666 39.0 36.0 41.0 32.0 41.0 28-29 37.31734602361912 39.0 36.0 41.0 31.5 41.0 30-31 37.18875004860993 39.0 36.0 41.0 31.0 41.0 32-33 37.058639045619095 39.0 36.0 40.5 31.0 41.0 34-35 36.907535456526816 39.0 35.5 40.0 30.0 41.0 36-37 36.761098641926864 39.0 35.0 40.0 30.0 41.0 38-39 36.59828558291264 38.0 35.0 40.0 30.0 41.0 40-41 36.43296911236495 38.0 35.0 40.0 30.0 41.0 42-43 36.375599669805986 38.0 35.0 40.0 30.0 41.0 44-45 36.410682010612874 38.0 35.0 40.0 30.0 41.0 46-47 36.28457359367046 38.0 35.0 40.0 30.0 41.0 48-49 36.14537109706431 38.0 35.0 40.0 29.5 41.0 50-51 35.94906529728067 38.0 34.5 40.0 28.5 41.0 52-53 36.01313865718154 38.0 35.0 40.0 29.0 41.0 54-55 36.007302206183894 38.0 35.0 40.0 29.0 41.0 56-57 35.84656401795563 38.0 34.0 40.0 28.0 41.0 58-59 35.635317237258896 38.0 34.0 40.0 28.0 41.0 60-61 35.37515450225197 38.0 34.0 40.0 28.0 41.0 62-63 35.09179455498048 37.0 34.0 40.0 28.0 41.0 64-65 34.76728906161618 37.0 33.5 40.0 27.0 41.0 66-67 34.42055695272286 36.0 33.0 39.0 26.0 41.0 68-69 34.050997840393265 36.0 33.0 39.0 26.0 41.0 70-71 33.66126450122674 35.5 32.0 39.0 26.0 40.0 72-73 33.264541053739606 35.0 32.0 38.0 26.0 40.0 74-75 32.8531572924621 35.0 32.0 37.0 25.0 39.5 76-77 31.493755225567764 34.0 30.5 36.0 23.0 39.0 78-79 32.14019386304019 34.5 31.0 36.0 25.0 39.0 80-81 32.02106969913103 35.0 31.0 36.0 25.0 38.5 82-83 31.7120016156174 34.5 31.0 36.0 24.0 37.0 84-85 31.390068747702074 34.0 31.0 35.0 23.5 37.0 86-87 31.043734798348325 34.0 31.0 35.0 22.5 36.5 88-89 30.724014731902965 34.0 31.0 35.0 20.5 36.0 90-91 30.4018737803326 34.0 30.0 35.0 20.0 36.0 92-93 30.03944618703688 34.0 30.0 35.0 18.0 35.5 94-95 29.668460428421433 34.0 30.0 35.0 14.0 35.0 96-97 29.2754862981857 34.0 29.5 35.0 4.5 35.0 98-99 28.800526489761864 34.0 29.0 35.0 2.0 35.0 100-101 27.680652793833787 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35475478123812465 1101 2 0.3599444853937932 1101 3 0.3847267814818842 1101 4 0.27634506432465145 1101 5 0.3164188129840255 1101 6 0.27558025082890225 1101 7 0.30956167655885025 1101 8 0.3254985188861923 1101 9 0.39606237354735896 1101 10-11 0.3871568114955579 1101 12-13 0.36771884040881275 1101 14-15 0.49151727129041234 1101 16-17 0.43703755188028026 1101 18-19 0.36117402628430284 1101 20-21 0.43162460234869826 1101 22-23 0.4864548775427622 1101 24-25 0.4117913650849587 1101 26-27 0.4602705776132652 1101 28-29 0.545045097577237 1101 30-31 0.5043366045175546 1101 32-33 0.42278780977937913 1101 34-35 0.4268097407785163 1101 36-37 0.4425115647382043 1101 38-39 0.5032527183340392 1101 40-41 0.4875680413611718 1101 42-43 0.4343124522202828 1101 44-45 0.42549923831064973 1101 46-47 0.445028409699205 1101 48-49 0.3641153869328235 1101 50-51 0.3765012631598026 1101 52-53 0.3993680524982324 1101 54-55 0.47025187101078103 1101 56-57 0.4934644528855969 1101 58-59 0.4454542046921972 1101 60-61 0.4276542411168336 1101 62-63 0.40587841241185174 1101 64-65 0.4217307425254688 1101 66-67 0.46562312832323016 1101 68-69 0.5156556575125748 1101 70-71 0.43868327443271227 1101 72-73 0.4517104331408177 1101 74-75 0.38325278836041576 1101 76-77 0.313292845113601 1101 78-79 0.4304178350496528 1101 80-81 0.4217969471389793 1101 82-83 0.4169081455892645 1101 84-85 0.6134034124580694 1101 86-87 0.5318888984676171 1101 88-89 0.4615613628510573 1101 90-91 0.5721916648192789 1101 92-93 0.5504750245859675 1101 94-95 0.5842482801203452 1101 96-97 0.541083107769218 1101 98-99 0.5966296031787195 1101 100-101 0.6888329571307032 1102 1 0.3025127505398828 1102 2 0.29811235868713126 1102 3 0.3738189658486597 1102 4 0.25045905208983044 1102 5 0.2626140242785624 1102 6 0.21223051211870114 1102 7 0.2292845110912083 1102 8 0.2272369969788457 1102 9 0.37020184327185035 1102 10-11 0.30210563397815804 1102 12-13 0.24806800829009745 1102 14-15 0.3969335862304604 1102 16-17 0.39297752298962507 1102 18-19 0.4245597395253 1102 20-21 0.3966829229445423 1102 22-23 0.3644626077733122 1102 24-25 0.3575081608502657 1102 26-27 0.39111231773841837 1102 28-29 0.41209938981320704 1102 30-31 0.3678943997417079 1102 32-33 0.3692103580055459 1102 34-35 0.44108405859274313 1102 36-37 0.40245866922101214 1102 38-39 0.3778498674210624 1102 40-41 0.37057400201827306 1102 42-43 0.4061126628364278 1102 44-45 0.4003350476685483 1102 46-47 0.3762801985016253 1102 48-49 0.39899377428200467 1102 50-51 0.34773297583671336 1102 52-53 0.3651431262412501 1102 54-55 0.40284595314202676 1102 56-57 0.44580111489196383 1102 58-59 0.5099194333201922 1102 60-61 0.503942387505127 1102 62-63 0.46971374377342556 1102 64-65 0.4428637786649716 1102 66-67 0.4607400049626449 1102 68-69 0.4051465164058925 1102 70-71 0.39353497787692504 1102 72-73 0.46179079010571655 1102 74-75 0.39327901204037374 1102 76-77 0.3382010729041802 1102 78-79 0.4429816922235972 1102 80-81 0.5060109165345423 1102 82-83 0.4550147643681406 1102 84-85 0.3715027662109449 1102 86-87 0.2948612196356777 1102 88-89 0.48322330208075215 1102 90-91 0.5348374879037756 1102 92-93 0.468202847815256 1102 94-95 0.4069289476882325 1102 96-97 0.37240595770254004 1102 98-99 0.48050440845799613 1102 100-101 0.6949541150060377 1103 1 0.2559524691026027 1103 2 0.30182940904287037 1103 3 0.34499565197931403 1103 4 0.18996727817660997 1103 5 0.3008750914114202 1103 6 0.2531754550830314 1103 7 0.2532448199310906 1103 8 0.23146390529533534 1103 9 0.4138124230868456 1103 10-11 0.3335725459296981 1103 12-13 0.3457030192198829 1103 14-15 0.4706254241370047 1103 16-17 0.4500034860562323 1103 18-19 0.3925944302629034 1103 20-21 0.40456575406484063 1103 22-23 0.47410162598368544 1103 24-25 0.44740690214731416 1103 26-27 0.40146859612635666 1103 28-29 0.4252154417269409 1103 30-31 0.4377032223730808 1103 32-33 0.48378658129264096 1103 34-35 0.4119115932662325 1103 36-37 0.5295867188539845 1103 38-39 0.5242939354168641 1103 40-41 0.5117740627266159 1103 42-43 0.45127969696707027 1103 44-45 0.31017299334349957 1103 46-47 0.4520196623094179 1103 48-49 0.42343143451200405 1103 50-51 0.48358649627747496 1103 52-53 0.49381135238980534 1103 54-55 0.525034217519881 1103 56-57 0.5269006572430825 1103 58-59 0.5667066900482851 1103 60-61 0.5707696515847047 1103 62-63 0.4810068046526652 1103 64-65 0.5335885876723196 1103 66-67 0.5335644800471044 1103 68-69 0.5443278050845137 1103 70-71 0.46325289365269384 1103 72-73 0.5847617555849425 1103 74-75 0.591448688641421 1103 76-77 0.4748423199719376 1103 78-79 0.6109655001271221 1103 80-81 0.685786967100448 1103 82-83 0.6143461738321996 1103 84-85 0.6993566157378268 1103 86-87 0.6941728525532405 1103 88-89 0.709855747859681 1103 90-91 0.6681927705267121 1103 92-93 0.6053848904698107 1103 94-95 0.6636111133210711 1103 96-97 0.6895782057302249 1103 98-99 0.7603476588816775 1103 100-101 0.8931843737056333 1104 1 0.16200153572311393 1104 2 0.22794368319693703 1104 3 0.21123132298260217 1104 4 0.14588948974424198 1104 5 0.13661499194677873 1104 6 0.20663994772888117 1104 7 0.12239416319513197 1104 8 0.13006442090951964 1104 9 0.21124870374105598 1104 10-11 0.2348970966824453 1104 12-13 0.1986334673053065 1104 14-15 0.2218840715958379 1104 16-17 0.26623985403200834 1104 18-19 0.2697172720876111 1104 20-21 0.33565254507866626 1104 22-23 0.35222319301541205 1104 24-25 0.2997488024240056 1104 26-27 0.2714148456083194 1104 28-29 0.3098066346371624 1104 30-31 0.2683731935378191 1104 32-33 0.22489131540854856 1104 34-35 0.3020161233813212 1104 36-37 0.33141392616281706 1104 38-39 0.3528249040861908 1104 40-41 0.35586651788431567 1104 42-43 0.3057866786313639 1104 44-45 0.41193082461725083 1104 46-47 0.3656680078118981 1104 48-49 0.343353223290066 1104 50-51 0.33389950359011067 1104 52-53 0.362845798224555 1104 54-55 0.29779090854664503 1104 56-57 0.3703784432754418 1104 58-59 0.31648099217579784 1104 60-61 0.3706657541994218 1104 62-63 0.3688408226554003 1104 64-65 0.4289691873765378 1104 66-67 0.40760810955495685 1104 68-69 0.3824347272091728 1104 70-71 0.26982375629977895 1104 72-73 0.36910946873337735 1104 74-75 0.3731666639981768 1104 76-77 0.3683941972097493 1104 78-79 0.346962335023548 1104 80-81 0.3388579005963024 1104 82-83 0.24883033601127735 1104 84-85 0.22250241135131432 1104 86-87 0.36329021993180177 1104 88-89 0.33658787297000003 1104 90-91 0.40922393465136864 1104 92-93 0.40846409066539735 1104 94-95 0.3425170410659959 1104 96-97 0.4124698851757316 1104 98-99 0.5088806227105032 1104 100-101 0.649399937961828 1105 1 0.1457427432478191 1105 2 0.12649407101022803 1105 3 0.10567912386068556 1105 4 0.11828859864171193 1105 5 0.09997903863904156 1105 6 0.15558573067709602 1105 7 0.14367283484230597 1105 8 0.11273990458125382 1105 9 0.14969177555404656 1105 10-11 0.10929438535490021 1105 12-13 0.17972024898326566 1105 14-15 0.1380893948885884 1105 16-17 0.0926243926209338 1105 18-19 0.15865228077700522 1105 20-21 0.15230958534991146 1105 22-23 0.17235559028741676 1105 24-25 0.0932220130814514 1105 26-27 0.15157621123046994 1105 28-29 0.10087477523890698 1105 30-31 0.13885104252301517 1105 32-33 0.03383231264639619 1105 34-35 0.11034370234277446 1105 36-37 0.2205284281298745 1105 38-39 0.21332072396165813 1105 40-41 0.2636841086567756 1105 42-43 0.20657331957610126 1105 44-45 0.1437546971882142 1105 46-47 0.1527788869285942 1105 48-49 0.21996322699823878 1105 50-51 0.21127826301998454 1105 52-53 0.20734832415536175 1105 54-55 0.2729473080909841 1105 56-57 0.2305290299766085 1105 58-59 0.14570928122547144 1105 60-61 0.05992409555216227 1105 62-63 0.10861268669950164 1105 64-65 0.18017784440725393 1105 66-67 0.14637192653079012 1105 68-69 0.06142380941400205 1105 70-71 0.06407745967443645 1105 72-73 0.09895993509828571 1105 74-75 0.1667887608328158 1105 76-77 0.06938255246680924 1105 78-79 0.12736599908743074 1105 80-81 0.18335734367178702 1105 82-83 0.2265244849441892 1105 84-85 0.32696517952112103 1105 86-87 0.3449054636198632 1105 88-89 0.2968067079106049 1105 90-91 0.31103476540395647 1105 92-93 0.23229712648473821 1105 94-95 0.32954206645001705 1105 96-97 0.43286017582488157 1105 98-99 0.5029754104296913 1105 100-101 0.5107639225307672 1106 1 -0.06937603818710869 1106 2 -0.005662197933578028 1106 3 -0.0744388481639362 1106 4 0.062409789802828186 1106 5 0.050046363919236114 1106 6 0.12925118244010037 1106 7 0.09918402127979675 1106 8 0.08550745310570562 1106 9 -0.01614743601882651 1106 10-11 0.004485913292654686 1106 12-13 -0.03621107459330375 1106 14-15 0.16981825602795908 1106 16-17 0.12758818683964535 1106 18-19 0.004454344098256513 1106 20-21 0.042658449837766454 1106 22-23 0.019441476719499917 1106 24-25 0.1690825956743467 1106 26-27 0.2207501096769775 1106 28-29 0.18473242556446934 1106 30-31 0.12256019580105004 1106 32-33 0.08326100821305715 1106 34-35 0.06089642188640454 1106 36-37 0.0714751450449782 1106 38-39 0.06024198583009621 1106 40-41 -0.042688685519785 1106 42-43 -0.06621891490350862 1106 44-45 0.01731612166286567 1106 46-47 0.11639415593960223 1106 48-49 0.12936071025605855 1106 50-51 0.03377250430927603 1106 52-53 -0.07797216425187514 1106 54-55 -0.045904500933893644 1106 56-57 -0.024989957992211487 1106 58-59 0.08731396037624961 1106 60-61 0.19537441309784498 1106 62-63 0.07467544712771712 1106 64-65 0.10837859268323768 1106 66-67 -0.0032420694324528654 1106 68-69 0.01937633810924666 1106 70-71 -0.05080267139403816 1106 72-73 -0.06165822215115924 1106 74-75 -0.0990539100716461 1106 76-77 -0.17623358065569406 1106 78-79 -0.14965110871004583 1106 80-81 0.1082333204505872 1106 82-83 0.15002580968726775 1106 84-85 0.21829863092900226 1106 86-87 0.356157927277156 1106 88-89 0.18632655234167927 1106 90-91 0.24911196263861157 1106 92-93 0.14786416332125896 1106 94-95 0.3270590083862288 1106 96-97 0.393940805973795 1106 98-99 0.4988381826729906 1106 100-101 0.4053714807134092 1107 1 0.139015998272896 1107 2 0.15520186276257064 1107 3 0.11794232562751361 1107 4 0.19181048894166253 1107 5 0.08866019811674875 1107 6 0.005899949019969597 1107 7 0.10636033514055754 1107 8 0.09761495330294423 1107 9 0.015208163825398913 1107 10-11 0.005437371740555363 1107 12-13 0.04192887435038273 1107 14-15 0.08944378930393526 1107 16-17 0.200821716761169 1107 18-19 0.08818861367692676 1107 20-21 0.055143273462107345 1107 22-23 -0.030579940682024187 1107 24-25 -0.023548640842228963 1107 26-27 0.09111125636506046 1107 28-29 0.07909918341834299 1107 30-31 0.06779166296794159 1107 32-33 0.13454217387206313 1107 34-35 0.11401894318707662 1107 36-37 0.20470823325930354 1107 38-39 0.16088348682133358 1107 40-41 0.25968584039839726 1107 42-43 0.1967079307826154 1107 44-45 0.13547311231801729 1107 46-47 0.1680490016160192 1107 48-49 0.08754705712251365 1107 50-51 0.1833040090527689 1107 52-53 0.19295370306630133 1107 54-55 0.10250219665167748 1107 56-57 0.12848897311415186 1107 58-59 0.1955821636382069 1107 60-61 0.3343613616846639 1107 62-63 0.23429271953447994 1107 64-65 0.3083126222286765 1107 66-67 0.23454011602142089 1107 68-69 0.28019471224712333 1107 70-71 0.2991955597530449 1107 72-73 0.21148391778618247 1107 74-75 0.2195892328828819 1107 76-77 0.14256834299265364 1107 78-79 0.23256294930077104 1107 80-81 0.38802383968684495 1107 82-83 0.4549947637884877 1107 84-85 0.3773629765577766 1107 86-87 0.30805171765659267 1107 88-89 0.35072800962459283 1107 90-91 0.3837891230298709 1107 92-93 0.4216342123075769 1107 94-95 0.3031988581032259 1107 96-97 0.41691217232709477 1107 98-99 0.5055235189655534 1107 100-101 0.7071713093562053 1108 1 0.04260162276395718 1108 2 0.03986785715481744 1108 3 7.053693478553669E-5 1108 4 0.061744435280481014 1108 5 -0.04307084452448606 1108 6 0.027867674020370714 1108 7 0.07284341752933443 1108 8 -0.004503285295797355 1108 9 -0.07218211881266257 1108 10-11 -0.13972657591973814 1108 12-13 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#Quality Count 2 134.0 3 92.0 4 265.0 5 638.0 6 1142.0 7 2017.0 8 3640.0 9 5708.0 10 7819.0 11 10001.0 12 12732.0 13 15644.0 14 17957.0 15 20507.0 16 23810.0 17 27903.0 18 32476.0 19 37094.0 20 40205.0 21 43039.0 22 48032.0 23 57697.0 24 70946.0 25 89538.0 26 114098.0 27 144703.0 28 182804.0 29 225480.0 30 275206.0 31 334554.0 32 409090.0 33 505963.0 34 651941.0 35 907980.0 36 1164889.0 37 1655933.0 38 1557296.0 39 351876.0 40 799.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.163738186723457 30.743474096871303 29.402047059260894 22.69074065714435 2 22.137924497284914 29.613336709514115 27.8123276556932 20.43641113750778 3 21.13110231418632 28.618335578228404 28.391769101052095 21.858793006533176 4 21.612220351055356 28.527167033847718 28.40945034648258 21.451162268614347 5 22.147988667653895 28.62516643444284 28.528388092640295 20.698456805262975 6 22.13469280433428 28.66103743443119 27.59243710476426 21.611832656470263 7 22.335789018751058 28.83973172620058 28.01745052392669 20.80702873112167 8 21.528835904950622 28.940891543094104 28.033397734242843 21.49687481771243 9 21.168723001388813 28.63039037308558 28.636842254698415 21.564044370827197 10-11 21.29800189148138 28.997621479565765 28.027283025261013 21.67709360369184 12-13 21.2889665985078 29.136096952084905 27.880049226403177 21.694887223004127 14-15 21.4642815832301 29.158301246171003 27.691286458696414 21.686130711902486 16-17 21.6195339025299 28.941410942041212 27.688318618240494 21.750736537188395 18-19 21.5832623149425 28.96398069478192 27.705496878689246 21.747260111586332 20-21 21.672305884475403 28.888031831139543 27.738869847832536 21.700792436552515 22-23 21.641295834470558 28.868443594358567 27.740807475158213 21.749453096012658 24-25 21.620555815944005 28.878378217887956 27.66717329635296 21.83389266981508 26-27 21.81258163223877 28.770541495346563 27.743943851105556 21.672933021309106 28-29 21.822591207009147 28.798369177985407 27.700894285040967 21.678145329964476 30-31 21.78507828354556 28.822228622621243 27.66503611161839 21.727656982214807 32-33 21.813268484547216 28.88427780591577 27.547009118752925 21.755444590784094 34-35 21.846436413351196 28.842285225028657 27.574336099871573 21.73694226174858 36-37 21.812381754657313 28.89966166296107 27.546070456962145 21.74188612541947 38-39 21.8582785677816 28.872185651091247 27.521636238966448 21.747899542160706 40-41 21.812161321314207 28.92821426014589 27.53542785652353 21.72419656201637 42-43 21.858545733993665 28.951697400814265 27.472865582718818 21.71689128247325 44-45 21.82250842571892 28.984323934278372 27.479203542047227 21.713964097955483 46-47 21.837613752302833 28.989903470640883 27.43435990874239 21.7381228683139 48-49 21.87489659328922 28.964087798822952 27.426296392151944 21.734719215735883 50-51 21.915328668445248 28.95643485966942 27.411951646118155 21.716284825767172 52-53 21.845601614394 29.022559431450816 27.38480844815664 21.74703050599854 54-55 21.884732457643008 29.037712149554185 27.308580798104476 21.768974594698328 56-57 21.87870098351151 29.051433506914986 27.330420935502573 21.73944457407093 58-59 21.872623877116652 29.135057989205603 27.263629475383 21.728688658294743 60-61 21.85894735202377 29.138065484487846 27.245132843624198 21.757854319864187 62-63 21.87683617835276 29.150396254089827 27.241243060533588 21.73152450702383 64-65 21.86226209949849 29.23288904193726 27.235740359623534 21.669108498940712 66-67 21.854107787761347 29.245535606520555 27.218826165801634 21.681530439916465 68-69 21.827213400548192 29.31140675043114 27.21009725384657 21.651282595174095 70-71 21.828449889609896 29.346035855130005 27.165080048292356 21.660434206967746 72-73 21.831505210653702 29.33014009147196 27.16176462058525 21.676590077289084 74-75 21.891275290942122 29.363966125050688 27.13871509030103 21.60604349370616 76-77 21.817341991204252 29.428966968225012 27.15569032291136 21.598000717659367 78-79 21.818954957152553 29.433833485349847 27.109831270504554 21.637380286993043 80-81 21.785179753387162 29.470689695648993 27.098257083317794 21.645873467646048 82-83 21.831617270217325 29.525900807467913 27.03395156827572 21.608530354039036 84-85 21.798267980845885 29.525907593238436 27.058634288269783 21.6171901376459 86-87 21.778056825445244 29.59509504613116 27.022946287823004 21.6039018406006 88-89 21.763715051536348 29.66012520874246 27.016415853484126 21.559743886237065 90-91 21.720531271848696 29.661000798809095 26.99475380014328 21.623714129198934 92-93 21.747060574276496 29.71512875406089 26.945424443286146 21.59238622837647 94-95 21.69935446358522 29.760613556502236 26.95197804105663 21.588053938855914 96-97 21.707831444098684 29.777118811376923 26.94089707739526 21.57415266712913 98-99 21.696220400970077 29.83831231616607 26.90436039934385 21.561106883519994 100-101 21.7902474775864 29.78340518765202 26.81530479311613 21.611042541645457 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 148155.0 1 83286.5 2 13843.5 3 8422.0 4 6860.0 5 5859.5 6 5439.5 7 5423.0 8 5706.5 9 5403.0 10 4903.5 11 5289.5 12 9266.0 13 9239.5 14 5687.5 15 6129.5 16 6987.0 17 8868.5 18 12259.5 19 14199.5 20 16266.0 21 20373.5 22 25684.0 23 32211.0 24 40723.0 25 51213.5 26 63237.5 27 76749.0 28 88368.5 29 99750.0 30 114509.5 31 132161.0 32 150292.5 33 168526.5 34 187945.0 35 206646.5 36 224802.5 37 242031.0 38 255735.5 39 264901.5 40 277489.0 41 289882.0 42 295270.0 43 304826.5 44 316463.0 45 325927.5 46 332998.0 47 337038.0 48 342491.0 49 348102.0 50 347626.5 51 338923.5 52 329161.5 53 312885.0 54 285105.5 55 260235.0 56 234635.0 57 206070.0 58 186078.0 59 163425.0 60 132570.5 61 102169.5 62 74139.5 63 51499.5 64 35658.0 65 23680.5 66 15318.0 67 10382.0 68 6219.5 69 3061.0 70 1414.5 71 657.0 72 320.0 73 176.0 74 121.5 75 88.0 76 63.5 77 46.5 78 33.0 79 27.0 80 25.0 81 15.0 82 9.5 83 13.5 84 11.5 85 4.0 86 4.5 87 3.5 88 1.5 89 1.5 90 1.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1492766842015951 2 0.0 3 0.0 4 0.0028171665535380963 5 3.9771763108773124E-4 6 9.721986537700096E-4 7 0.0 8 1.767633915945472E-4 9 2.7619279936648E-4 10-11 1.3809639968324E-4 12-13 1.6571567961988802E-5 14-15 1.10477119746592E-5 16-17 5.5238559873296E-6 18-19 2.20954239493184E-5 20-21 9.39055517846032E-5 22-23 1.491441116578992E-4 24-25 5.5238559873296E-6 26-27 5.5238559873296E-6 28-29 0.0056011899711522145 30-31 0.0014251548447310367 32-33 2.927643673284688E-4 34-35 2.540973754171616E-4 36-37 2.540973754171616E-4 38-39 0.0012097244612251825 40-41 0.0032701227444991232 42-43 4.860993268850048E-4 44-45 5.137186068216529E-4 46-47 0.006269576545619097 48-49 0.006799866720402737 50-51 0.006926915408111319 52-53 0.009075695387182533 54-55 0.004717373013179479 56-57 0.0 58-59 7.18101278352848E-5 60-61 9.335316618587023E-4 62-63 7.678159822388144E-4 64-65 4.805754708976752E-4 66-67 6.021003026189264E-4 68-69 8.617215340234176E-4 70-71 0.0012373437411618304 72-73 0.002656974729905538 74-75 6.904819984162E-4 76-77 0.0 78-79 0.0 80-81 1.160009757339216E-4 82-83 2.099065275185248E-4 84-85 1.767633915945472E-4 86-87 0.002513354474234968 88-89 0.0013643924288704113 90-91 5.358140307709712E-4 92-93 5.5238559873296E-4 94-95 3.1485979127778723E-4 96-97 0.0010937234854912607 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9051648.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.25087471242146 #Duplication Level Percentage of deduplicated Percentage of total 1 90.63624519612745 63.67275505677461 2 5.2812784185470285 7.420288570055253 3 1.383283966635419 2.915307258954187 4 0.6693612347110025 1.880928489481375 5 0.38236654561450967 1.3430792145093153 6 0.25163056282813356 1.0606360285833185 7 0.18542285487031096 0.9118282422419607 8 0.14674080233809447 0.8246935776202943 9 0.1273109739390896 0.8049336551740205 >10 0.9040557307153295 11.639532206174117 >50 0.029986692167210515 1.3335383558193643 >100 0.0019336568377991267 0.20381113992509725 >500 4.792058359084943E-5 0.01885219231832655 >1k 1.9168233429075218E-4 0.45174565825669316 >5k 0.0 0.0 >10k+ 1.4376175071806414E-4 5.51807035411203 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 138469 1.529765629419085 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15178 0.16768217235137733 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10266 0.11341581113185135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.20954239493184E-5 0.0 2 1.10477119746592E-5 0.0 0.0 3.3143135923977603E-5 0.0 3 1.10477119746592E-5 0.0 0.0 4.41908478986368E-5 0.0 4 1.10477119746592E-5 0.0 0.0 4.41908478986368E-5 0.0 5 1.10477119746592E-5 0.0 0.0 4.41908478986368E-5 0.0 6 1.10477119746592E-5 0.0 0.0 5.5238559873296E-5 0.0 7 1.10477119746592E-5 0.0 0.0 6.628627184795521E-5 0.0 8 2.20954239493184E-5 0.0 0.0 7.73339838226144E-5 0.0 9 3.3143135923977603E-5 0.0 0.0 7.73339838226144E-5 0.0 10-11 3.86669919113072E-5 0.0 0.0 8.838169579727359E-5 0.0 12-13 5.5238559873296E-5 0.0 0.0 1.2152483172125121E-4 0.0 14-15 8.285783980994401E-5 0.0 0.0 1.3809639968324E-4 0.0 16-17 9.94294077719328E-5 0.0 0.0 1.4914411165789922E-4 0.0 18-19 1.3809639968324E-4 0.0 0.0 1.65715679619888E-4 1.10477119746592E-5 20-21 1.4914411165789922E-4 0.0 0.0 1.767633915945472E-4 1.10477119746592E-5 22-23 1.712395356072176E-4 0.0 0.0 1.878111035692064E-4 1.10477119746592E-5 24-25 1.93334959556536E-4 0.0 0.0 1.9333495955653598E-4 1.10477119746592E-5 26-27 2.1543038350585442E-4 0.0 0.0 1.988588155438656E-4 1.10477119746592E-5 28-29 2.264780954805136E-4 0.0 0.0 1.988588155438656E-4 1.10477119746592E-5 30-31 2.320019514678432E-4 0.0 0.0 1.988588155438656E-4 1.10477119746592E-5 32-33 2.4304966344250242E-4 0.0 0.0 2.043826715311952E-4 1.10477119746592E-5 34-35 2.596212314044912E-4 0.0 0.0 2.4304966344250242E-4 1.10477119746592E-5 36-37 2.6514508739182083E-4 0.0 0.0 2.540973754171616E-4 1.10477119746592E-5 38-39 2.872405113411392E-4 0.0 0.0 2.7619279936648E-4 1.10477119746592E-5 40-41 2.9828822331579843E-4 0.0 0.0 3.203836472651168E-4 1.10477119746592E-5 42-43 3.1485979127778723E-4 0.0 0.0 3.203836472651168E-4 1.10477119746592E-5 44-45 3.3695521522710556E-4 0.0 0.0 3.259075032524464E-4 1.10477119746592E-5 46-47 3.8666991911307197E-4 0.0 0.0 3.3695521522710556E-4 1.10477119746592E-5 48-49 4.529561909610272E-4 0.0 0.0 3.424790712144352E-4 1.10477119746592E-5 50-51 4.916231828723344E-4 0.0 0.0 3.59050639176424E-4 1.10477119746592E-5 52-53 5.192424628089824E-4 0.0 0.0 3.645744951637536E-4 1.10477119746592E-5 54-55 5.965764466315969E-4 0.0 0.0 3.756222071384128E-4 1.10477119746592E-5 56-57 7.015297103908592E-4 0.0 0.0 3.8114606312574244E-4 1.10477119746592E-5 58-59 8.451499660614289E-4 0.0 0.0 3.921937751004016E-4 1.10477119746592E-5 60-61 9.77722509757339E-4 0.0 0.0 4.198130550370496E-4 1.10477119746592E-5 62-63 0.001077151917529272 0.0 0.0 4.3638462299903837E-4 1.10477119746592E-5 64-65 0.0011821051812885344 0.0 0.0 4.584800469483568E-4 1.10477119746592E-5 66-67 0.0013367731489337632 0.0 0.0 4.97147038859664E-4 1.10477119746592E-5 68-69 0.0015080126845409807 0.0 0.0 5.192424628089824E-4 1.10477119746592E-5 70-71 0.0017234430680468354 0.0 0.0 5.302901747836417E-4 1.10477119746592E-5 72-73 0.0019664927314893377 0.0 0.0 5.358140307709712E-4 1.10477119746592E-5 74-75 0.002170875403020533 0.0 0.0 5.5238559873296E-4 1.10477119746592E-5 76-77 0.00249125905028565 0.0 0.0 5.5238559873296E-4 1.10477119746592E-5 78-79 0.002806118841563437 0.0 0.0 5.579094547202896E-4 1.10477119746592E-5 80-81 0.0033364090163470785 0.0 0.0 5.744810226822784E-4 1.10477119746592E-5 82-83 0.004037938726737938 0.0 0.0 5.910525906442672E-4 1.10477119746592E-5 84-85 0.0049383272526726625 0.0 0.0 6.186718705809152E-4 1.10477119746592E-5 86-87 0.006319291249505063 0.0 0.0 6.794342864415408E-4 1.6571567961988802E-5 88-89 0.008075877453475875 0.0 0.0 7.070535663781888E-4 2.20954239493184E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 375 0.0 39.26657 3 CGCGTAG 260 0.0 27.403402 54-55 CGTACGC 290 0.0 26.20553 20-21 CTCGCGT 290 0.0 24.569721 52-53 ACGTACG 340 0.0 22.351776 20-21 TCGCGTA 355 0.0 21.40911 52-53 ACTCGCG 345 0.0 21.341827 50-51 CAACGCA 6025 0.0 19.747934 12-13 ATCAACG 6180 0.0 19.022068 10-11 CGCTAGT 410 0.0 18.536335 90-91 AGTGGTA 7855 0.0 17.83842 5 TCAACGC 5990 0.0 17.405186 10-11 GGTATCA 8470 0.0 16.935457 8 CGTAGCG 425 0.0 16.76411 56-57 GTATCAA 8385 0.0 16.653965 9 AACGCAG 6465 0.0 16.310057 12-13 AAGCAGT 9225 0.0 15.416919 1 TGGTATC 9665 0.0 14.9889555 7 GTCACGT 1105 0.0 14.61535 4 ACGTGTA 1075 0.0 14.580717 7 >>END_MODULE