FastQCFastQC Report
Sat 18 Jun 2016
SRR578558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9144992
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117335712.830596243277196No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT288280.31523264317781796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104640.1144232821636148No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG101130.11058511587544308No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT96280.10528166673081836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2562450.091.903295
AAGCAGT2586950.091.647851
GTATCAA2586150.091.455029
GGTATCA2584300.091.4489448
TGGTATC2582550.091.277237
GTGGTAT2600900.090.739386
AGCAGTG2611950.090.6727142
GCAGTGG2607550.090.638823
CAGTGGT2610750.090.284964
TCAACGC2578600.046.422712-13
AACGCAG2588400.046.29479614-15
CAACGCA2578700.046.26152812-13
ATCAACG2577800.046.24900410-11
ACGCAGA2592350.046.0574714-15
CGCAGAG2602300.046.0391416-17
TATCAAC2598250.045.9471710-11
CAGAGTA2585400.045.8539518-19
AGAGTAC2537950.045.67714318-19
GCAGAGT2614700.045.4910416-17
GAGTACT1498850.045.484920-21
Produced by FastQC (version 0.11.5)