##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578558_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9144992 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.44551684681627 31.0 31.0 33.0 27.0 34.0 2 30.745382062663367 31.0 31.0 34.0 27.0 34.0 3 30.850241203054086 31.0 31.0 34.0 27.0 34.0 4 34.27729504848118 37.0 35.0 37.0 30.0 37.0 5 34.31265636973767 37.0 35.0 37.0 30.0 37.0 6 34.302998187423235 37.0 35.0 37.0 30.0 37.0 7 34.19502368072055 37.0 35.0 37.0 30.0 37.0 8 34.154363065599185 37.0 35.0 37.0 30.0 37.0 9 35.57650044964501 39.0 35.0 39.0 30.0 39.0 10-11 35.54267696461626 39.0 35.0 39.0 30.0 39.0 12-13 35.61404263666934 39.0 35.0 39.0 30.0 39.0 14-15 36.63801258656103 40.0 36.0 41.0 30.0 41.0 16-17 36.607871882228 40.0 36.0 41.0 30.0 41.0 18-19 36.581977327044136 40.0 36.0 41.0 30.0 41.0 20-21 36.46084955569124 39.5 36.0 41.0 29.5 41.0 22-23 36.159232889432815 39.0 36.0 40.5 28.0 41.0 24-25 36.055456308764406 39.0 36.0 40.0 27.0 41.0 26-27 36.00128879281688 39.0 36.0 40.0 27.0 41.0 28-29 35.69026577606628 39.0 35.0 41.0 27.0 41.0 30-31 35.417949791536174 38.5 34.5 40.5 25.5 41.0 32-33 35.05203082736431 38.0 34.0 40.0 25.0 41.0 34-35 34.85623120282664 38.0 34.0 40.0 24.5 41.0 36-37 34.86564198197221 38.0 34.0 40.0 25.0 41.0 38-39 34.1527462243816 37.0 33.5 40.0 24.0 41.0 40-41 34.11520283451314 37.0 33.0 40.0 24.0 41.0 42-43 33.93128282670996 37.0 33.0 40.0 23.0 41.0 44-45 33.771924130715476 37.0 33.0 40.0 22.5 41.0 46-47 33.56130234996378 37.0 33.0 40.0 22.0 41.0 48-49 33.200796567126574 37.0 33.0 40.0 19.0 41.0 50-51 31.922511031174224 35.5 31.0 39.0 12.0 40.5 52-53 31.501027392916257 35.5 31.0 39.0 5.0 40.0 54-55 31.149548791294734 36.0 30.0 40.0 2.0 41.0 56-57 30.652279083459014 36.0 28.5 40.0 2.0 41.0 58-59 30.32888142493728 36.0 28.0 40.0 2.0 41.0 60-61 30.018105756680814 35.5 28.0 39.0 2.0 41.0 62-63 29.68174236784461 35.0 27.5 39.0 2.0 41.0 64-65 29.348925400918887 35.0 27.0 39.0 2.0 41.0 66-67 29.047448483279155 35.0 26.0 38.5 2.0 40.0 68-69 28.710136925215462 35.0 26.0 38.0 2.0 40.0 70-71 28.325397332222927 35.0 26.0 37.0 2.0 40.0 72-73 27.93729557117163 34.0 26.0 37.0 2.0 39.0 74-75 27.547597635952005 34.0 25.0 36.0 2.0 39.0 76-77 27.14700658021352 34.0 24.0 36.0 2.0 39.0 78-79 26.760397658084337 34.0 22.5 35.0 2.0 37.5 80-81 26.37577167918791 34.0 21.5 35.0 2.0 37.0 82-83 26.01351346179417 33.0 20.0 35.0 2.0 36.5 84-85 25.62154887614992 33.0 18.5 35.0 2.0 36.0 86-87 25.26746157897131 33.0 17.5 35.0 2.0 36.0 88-89 24.881634122807327 32.0 11.0 35.0 2.0 35.5 90-91 24.507465889527296 32.0 7.0 35.0 2.0 35.0 92-93 24.150613253680266 32.0 2.0 35.0 2.0 35.0 94-95 23.70727317202683 32.0 2.0 35.0 2.0 35.0 96-97 23.227056294855153 31.0 2.0 35.0 2.0 35.0 98-99 22.62987179212404 31.0 2.0 34.0 2.0 35.0 100-101 21.088992259369938 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.480857076347462 1101 2 0.49612893766127186 1101 3 0.5074786696667566 1101 4 0.6252666406245453 1101 5 0.6238274608342351 1101 6 0.5175115574876372 1101 7 0.5899041950074633 1101 8 0.5739146840949303 1101 9 0.5673972885923817 1101 10-11 0.6182548927823959 1101 12-13 0.5738128742458883 1101 14-15 0.7392248188234234 1101 16-17 0.6673830170270918 1101 18-19 0.7280633832938506 1101 20-21 0.781349219579802 1101 22-23 0.8120821709184227 1101 24-25 0.9375827298569774 1101 26-27 0.915342889793159 1101 28-29 0.8897543702777426 1101 30-31 0.9653625783781408 1101 32-33 1.0153739588378201 1101 34-35 0.9143087170065627 1101 36-37 0.8776311591501624 1101 38-39 1.0997081237431985 1101 40-41 1.0233141302918014 1101 42-43 1.220391542082936 1101 44-45 1.2275896735137266 1101 46-47 1.1762735411246723 1101 48-49 1.2564986914198641 1101 50-51 1.304750590134855 1101 52-53 1.3148135089499107 1101 54-55 1.2710779985334177 1101 56-57 1.3405697502603822 1101 58-59 1.4098785608938549 1101 60-61 1.4685388768134437 1101 62-63 1.4290632356704585 1101 64-65 1.4293060391995631 1101 66-67 1.4384153770539605 1101 68-69 1.523927744615019 1101 70-71 1.4026272194435485 1101 72-73 1.5042668411932105 1101 74-75 1.5073796054362099 1101 76-77 1.4243539060280241 1101 78-79 1.40914904101313 1101 80-81 1.4422541252356993 1101 82-83 1.4904803177642343 1101 84-85 1.5652004632571206 1101 86-87 1.574814376048451 1101 88-89 1.712358282280018 1101 90-91 1.8472115067054062 1101 92-93 1.8316766347880673 1101 94-95 1.9071921145286197 1101 96-97 2.0811830807236973 1101 98-99 2.317790068638061 1101 100-101 2.7344522346343645 1102 1 0.3907040175068133 1102 2 0.31262359659537964 1102 3 0.3307798708674916 1102 4 0.4371865611009724 1102 5 0.39196919482163395 1102 6 0.32280996903828907 1102 7 0.45112032254547074 1102 8 0.5411770583673956 1102 9 0.41542447357998924 1102 10-11 0.49929798009564763 1102 12-13 0.5173466344625339 1102 14-15 0.6479273487815291 1102 16-17 0.6003001306370237 1102 18-19 0.5262491994273404 1102 20-21 0.48484854918645937 1102 22-23 0.5725066599388455 1102 24-25 0.7089552506534318 1102 26-27 0.7376687097507073 1102 28-29 0.6694651830547613 1102 30-31 0.8177824610351081 1102 32-33 0.8086625058515935 1102 34-35 0.9148848018419571 1102 36-37 0.6733125474758168 1102 38-39 0.7909934794890958 1102 40-41 0.7375997460572634 1102 42-43 0.921126030236131 1102 44-45 0.8354467224071769 1102 46-47 0.8258720797344239 1102 48-49 0.909920585343059 1102 50-51 0.7334378846754923 1102 52-53 0.8342892994585327 1102 54-55 0.8305763528881407 1102 56-57 0.661422206065982 1102 58-59 0.7536293698374514 1102 60-61 0.8115481792676746 1102 62-63 0.7551584649481349 1102 64-65 0.7248881930053841 1102 66-67 0.6810422202107844 1102 68-69 0.8227321016532834 1102 70-71 0.7639545824991103 1102 72-73 0.7792917477276653 1102 74-75 0.9552136698020988 1102 76-77 0.838570189400432 1102 78-79 0.81929906740055 1102 80-81 0.9359604485927981 1102 82-83 1.0653911550925024 1102 84-85 1.1700748916888237 1102 86-87 1.1132698214115706 1102 88-89 1.2243833805102469 1102 90-91 1.232416458746858 1102 92-93 1.1239054179662062 1102 94-95 1.2419929931617482 1102 96-97 1.4518549230530695 1102 98-99 1.5541446448333858 1102 100-101 1.9676350007041208 1103 1 0.22610998443928665 1103 2 0.21620007102255911 1103 3 0.14308142105283395 1103 4 0.23249144213716022 1103 5 0.186160025921545 1103 6 0.08436865908021929 1103 7 0.32592095556887557 1103 8 0.2119497974782476 1103 9 0.4274106372691904 1103 10-11 0.3562603702442573 1103 12-13 0.20875139132729714 1103 14-15 0.44555666392586346 1103 16-17 0.4061627820918119 1103 18-19 0.41431556889837395 1103 20-21 0.5636573966989431 1103 22-23 0.5681183360373367 1103 24-25 0.4889628200231044 1103 26-27 0.6385851918714991 1103 28-29 0.5732634993212642 1103 30-31 0.4556402801892929 1103 32-33 0.6041202628380233 1103 34-35 0.6504982524876226 1103 36-37 0.6335432573626747 1103 38-39 0.39995363000445394 1103 40-41 0.5029616165471964 1103 42-43 0.3125052589386996 1103 44-45 0.416638128066559 1103 46-47 0.608598949262074 1103 48-49 0.683914385445604 1103 50-51 0.6315865068697128 1103 52-53 0.5143039248216184 1103 54-55 0.6257161096134283 1103 56-57 0.4831305441169853 1103 58-59 0.7246114211249157 1103 60-61 0.6653067381759605 1103 62-63 0.6396440818564564 1103 64-65 0.7447067086353449 1103 66-67 0.6525178394093665 1103 68-69 0.7508367680559935 1103 70-71 0.8230757550036145 1103 72-73 0.834701877930673 1103 74-75 0.9923634165013127 1103 76-77 0.9152490645766314 1103 78-79 0.9985149824398221 1103 80-81 0.9192855890035538 1103 82-83 1.084325233594999 1103 84-85 1.0209357111639292 1103 86-87 1.2296556665730698 1103 88-89 1.316616560229214 1103 90-91 1.0410964792912871 1103 92-93 1.201267587574005 1103 94-95 1.2746677632031727 1103 96-97 1.518051686344652 1103 98-99 1.6439002449128068 1103 100-101 1.9411007265091555 1104 1 0.17188914786904874 1104 2 0.22159982647556475 1104 3 0.217219804470286 1104 4 0.2430062770164909 1104 5 0.08240075894516963 1104 6 0.11050534816083513 1104 7 0.34713394982905754 1104 8 0.21634632717459112 1104 9 0.3520479754454442 1104 10-11 0.3669005654702744 1104 12-13 0.2848654435190454 1104 14-15 0.38454690433992056 1104 16-17 0.5145435757205661 1104 18-19 0.6222040101942312 1104 20-21 0.46964879811099536 1104 22-23 0.3897331350749269 1104 24-25 0.4166351304777507 1104 26-27 0.49716200037885727 1104 28-29 0.6614648784565702 1104 30-31 0.5304862378754578 1104 32-33 0.6969864702502306 1104 34-35 0.7417761906100324 1104 36-37 0.7612460933189737 1104 38-39 0.5899058524496184 1104 40-41 0.6295119433244096 1104 42-43 0.7461144364822161 1104 44-45 0.9940357947520866 1104 46-47 0.8068213532653701 1104 48-49 0.8327135156402932 1104 50-51 0.751862985598752 1104 52-53 0.5828936123468011 1104 54-55 0.6917414835079505 1104 56-57 0.7472821152371161 1104 58-59 0.6743516113776771 1104 60-61 0.6155455402043621 1104 62-63 0.6385076071403155 1104 64-65 0.743738467161478 1104 66-67 0.5976936097066847 1104 68-69 0.624259860083523 1104 70-71 0.6996085038936357 1104 72-73 0.5170240968945556 1104 74-75 0.6153811881739912 1104 76-77 0.6941638315452927 1104 78-79 0.7184283277432684 1104 80-81 0.6503426288187519 1104 82-83 0.6587467496148847 1104 84-85 0.655191754956018 1104 86-87 0.6925486609704414 1104 88-89 0.8606590284046618 1104 90-91 1.0203503708640156 1104 92-93 0.9992421862408278 1104 94-95 1.0283860436913734 1104 96-97 1.1941897473579282 1104 98-99 1.4000934061714858 1104 100-101 1.6931758604012046 1105 1 -0.18666411984281694 1105 2 0.06659228956060304 1105 3 -0.006350339377252823 1105 4 0.0217925729389421 1105 5 0.018718765987316033 1105 6 0.019765392034635454 1105 7 0.1352022934477617 1105 8 0.1330805168454603 1105 9 0.050077196798589796 1105 10-11 0.12977176891666886 1105 12-13 0.032570832528627136 1105 14-15 -0.0895390774136402 1105 16-17 0.13638816181100566 1105 18-19 -0.039814767247079885 1105 20-21 -0.07194356094338161 1105 22-23 0.05770403302934568 1105 24-25 0.28647358411325996 1105 26-27 0.24892167156074407 1105 28-29 0.40767986907119536 1105 30-31 0.35361584373588784 1105 32-33 0.4581811543482601 1105 34-35 0.36748068792826416 1105 36-37 0.5990877756433122 1105 38-39 0.3326618929431149 1105 40-41 0.28887334093521133 1105 42-43 0.5193230807190616 1105 44-45 0.5144555496846621 1105 46-47 0.25411196964470406 1105 48-49 0.4423881532983742 1105 50-51 0.4229160917723327 1105 52-53 0.27640196549165097 1105 54-55 0.29214741768617714 1105 56-57 0.2630548520972482 1105 58-59 0.31293453521145054 1105 60-61 0.24670370280326281 1105 62-63 0.3724781051720889 1105 64-65 0.5340308006928112 1105 66-67 0.40363041869781213 1105 68-69 0.4039350838948188 1105 70-71 0.3629369183861151 1105 72-73 0.2629241570791052 1105 74-75 0.21686657888203342 1105 76-77 0.2210613756556299 1105 78-79 0.3559653719341469 1105 80-81 0.3698910404589242 1105 82-83 0.5730036977227861 1105 84-85 0.6048260438113999 1105 86-87 0.59790509174044 1105 88-89 0.4849161888200264 1105 90-91 0.7218968518908859 1105 92-93 0.8446904469119332 1105 94-95 0.9120376074512286 1105 96-97 1.1119187532651509 1105 98-99 1.3701259249826236 1105 100-101 1.7890505244592632 1106 1 0.24028886685487194 1106 2 0.2810056258323428 1106 3 0.2709135283366422 1106 4 0.09063158317760411 1106 5 0.3144475390098478 1106 6 0.20581792568781765 1106 7 0.3316592253176722 1106 8 0.3184428259995613 1106 9 0.39416892205274934 1106 10-11 0.17708005594602128 1106 12-13 0.344300674938502 1106 14-15 0.3095199249491216 1106 16-17 0.3845434234857521 1106 18-19 0.38349272858014416 1106 20-21 0.34510657381387944 1106 22-23 0.3826499314230958 1106 24-25 0.633959620660292 1106 26-27 0.4787867517286699 1106 28-29 0.5276578129572584 1106 30-31 0.6175559490127327 1106 32-33 0.5218494871155954 1106 34-35 0.5122097102336554 1106 36-37 0.5952634965765782 1106 38-39 0.6340955855087032 1106 40-41 0.48664352381489806 1106 42-43 0.7167187973892268 1106 44-45 0.6296497464519248 1106 46-47 0.6153217502035346 1106 48-49 0.7651702584018096 1106 50-51 0.5445958879384314 1106 52-53 0.5661264988991661 1106 54-55 0.7072519268157116 1106 56-57 0.5877743185596707 1106 58-59 0.6014526208635935 1106 60-61 0.5347721761083228 1106 62-63 0.6294251939818132 1106 64-65 0.6981275657961028 1106 66-67 0.6448489331887366 1106 68-69 0.6491677298336782 1106 70-71 0.7164338330591171 1106 72-73 0.6838011901795333 1106 74-75 0.680940628536284 1106 76-77 0.7676624580490348 1106 78-79 0.8427774957518608 1106 80-81 0.838247742555069 1106 82-83 0.9296113192752777 1106 84-85 1.0396123831106614 1106 86-87 1.0825369388903034 1106 88-89 1.0636988755834693 1106 90-91 0.9875950787310579 1106 92-93 1.1751123491207949 1106 94-95 1.1968944414143898 1106 96-97 1.3181260771233276 1106 98-99 1.3961837062206541 1106 100-101 1.6328228786287866 1107 1 0.05457947007859332 1107 2 0.10131057253104814 1107 3 0.10129167312173237 1107 4 -0.006500811853769051 1107 5 0.10211677396983987 1107 6 0.15173548977499962 1107 7 0.21899884009093995 1107 8 0.20522886837294863 1107 9 0.1724910795400163 1107 10-11 0.20018529567882126 1107 12-13 0.19170447617954522 1107 14-15 0.23803986857848258 1107 16-17 0.16928547426681462 1107 18-19 0.13238204388706976 1107 20-21 0.17565534577308028 1107 22-23 0.32018141273029954 1107 24-25 0.3694117708001201 1107 26-27 0.2865758228693025 1107 28-29 0.4232236001475087 1107 30-31 0.4481767329782329 1107 32-33 0.40828792383828016 1107 34-35 0.35827917675447196 1107 36-37 0.2705852005689664 1107 38-39 0.37066372478537346 1107 40-41 0.2572175995358066 1107 42-43 0.3081238285273358 1107 44-45 0.3009868688381303 1107 46-47 0.41372652102997876 1107 48-49 0.49364154468679544 1107 50-51 0.3829974976833306 1107 52-53 0.43209064603958325 1107 54-55 0.5215248143843816 1107 56-57 0.3074914379153775 1107 58-59 0.4037495475454982 1107 60-61 0.3829625027886685 1107 62-63 0.4270889777219615 1107 64-65 0.5384641319441883 1107 66-67 0.5807337111726696 1107 68-69 0.5219548432406356 1107 70-71 0.579954995145652 1107 72-73 0.598606540296565 1107 74-75 0.5780994810323854 1107 76-77 0.6291601825883504 1107 78-79 0.5399122435171932 1107 80-81 0.6655198576067818 1107 82-83 0.6575783002729558 1107 84-85 0.7946552201365655 1107 86-87 0.8139319126303413 1107 88-89 0.8990986072422871 1107 90-91 0.8876408814968393 1107 92-93 0.9780849421444415 1107 94-95 0.9453409732686815 1107 96-97 1.1036292319453125 1107 98-99 1.2557456109681695 1107 100-101 1.534008160097791 1108 1 0.2446514904292485 1108 2 0.25943950480692735 1108 3 0.028596937926074872 1108 4 0.24869748849851447 1108 5 0.3207213500527075 1108 6 0.2774068324001746 1108 7 0.2600678121797486 1108 8 0.2007587784108935 1108 9 0.26526337935845135 1108 10-11 0.2577519450921315 1108 12-13 0.2933098773179452 1108 14-15 0.23124687494821927 1108 16-17 0.3794712915961185 1108 18-19 0.1347798160832525 1108 20-21 0.15927949894921767 1108 22-23 0.3329043715939548 1108 24-25 0.3625876336876317 1108 26-27 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2316 94-95 -4.3667152509611356 2316 96-97 -4.55592201487714 2316 98-99 -4.644572159132544 2316 100-101 -4.737209345619043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 141141.0 3 21498.0 4 17687.0 5 16228.0 6 17714.0 7 21089.0 8 25566.0 9 29857.0 10 34467.0 11 40839.0 12 42002.0 13 47842.0 14 54136.0 15 64745.0 16 82815.0 17 109181.0 18 156056.0 19 227321.0 20 295121.0 21 216851.0 22 131992.0 23 118754.0 24 123187.0 25 131441.0 26 144854.0 27 163231.0 28 188257.0 29 220502.0 30 259005.0 31 308966.0 32 373070.0 33 463507.0 34 591802.0 35 813135.0 36 1150982.0 37 1245747.0 38 907297.0 39 146947.0 40 160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.56301575418488 47.956033060819 24.249815578226034 17.231135606770078 2 16.301107662228976 49.593207666553 18.191889331204525 15.913795340013499 3 44.490100540225285 20.40752681204031 19.022055623325755 16.080317024408654 4 16.353923344941634 18.847672090367432 20.007768783151132 44.790635781539805 5 16.393522830882794 47.27470942164537 20.870153424495598 15.461614322976242 6 44.866055622614724 19.959868837158297 18.80284192723704 16.37123361298994 7 17.215489556950246 19.438378235200116 48.00293775762843 15.343194450221207 8 44.37633864059512 20.165694003711007 19.635496833012628 15.822470522681249 9 44.01892997341372 19.81088194387594 20.212051074723945 15.95813700798639 10-11 16.05896956953838 33.72371845411519 33.881637678330776 16.335674298015643 12-13 15.891956712828284 20.13393824869191 33.839038619215515 30.135066419264295 14-15 16.050960453541624 47.91950841044902 19.671176741694264 16.358354394315093 16-17 30.088341801602002 19.92060355262418 19.791713855461897 30.199340790311922 18-19 16.184725577703723 33.78518792340681 19.83148059329186 30.198605905597596 20-21 30.226207708458475 33.85222701078685 19.816271199792556 16.105294080962118 22-23 30.173159150168377 19.88080949244204 33.779698753666786 16.166332603722797 24-25 16.11511264385949 33.62504770541664 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50-51 19.577326526195975 22.577271168791725 38.09694372427549 19.748458580736806 52-53 19.949684462625946 23.725893275005102 36.93449582831651 19.389926434052438 54-55 20.46834249830254 24.31732324954248 35.45959415589832 19.754740096256654 56-57 20.49284113619367 25.0856048615247 34.29326923708072 20.128284765200906 58-59 20.6977173269892 25.77978970029554 33.33452216569738 20.187970807017884 60-61 21.003400127962216 25.972632363960557 32.867796060586286 20.15617144749094 62-63 21.07603549057745 25.783085603690743 32.896436035534336 20.24444287019747 64-65 20.44031530441203 25.462904994861642 33.12604851987517 20.97073118085116 66-67 20.695199345717384 25.21298535696954 33.81545943968518 20.27635585762789 68-69 20.573627350127776 24.976467749754587 33.882091898763385 20.567813001354256 70-71 20.35528788164729 24.961634925974856 34.52783908932523 20.155238103052632 72-73 19.98366701719312 24.686909683145057 34.50443810245309 20.824985197208733 74-75 20.35582578252096 25.047256564761035 33.64309126992093 20.95382638279707 76-77 20.807943976378198 25.555633164976804 33.12639331169908 20.51002954694592 78-79 20.765064476593246 25.610165414053693 32.942056562261406 20.68271354709166 80-81 20.473336833827 26.042141254054503 32.803392725577396 20.681129186541103 82-83 21.39636474936787 26.308269126710993 32.054925684915155 20.240440439005983 84-85 20.909326222788586 26.69489091693404 31.543701219711657 20.852081640565725 86-87 20.816124221456526 27.078551555620933 31.364607105466174 20.74071711745637 88-89 20.807301241980873 26.6254401556275 31.42591661277824 21.141341989613384 90-91 20.728675856122365 26.47563555880242 31.587558823423233 21.208129761651982 92-93 20.67948388367145 26.952167896426676 31.402298347618242 20.966049872283634 94-95 20.7759410798025 26.36391452965647 31.089015891186143 21.771128499354887 96-97 20.735104007553584 26.255037314259333 31.413523856016994 21.596334822170085 98-99 21.076214250078458 26.68313665496146 30.96730252831876 21.273346566641322 100-101 21.441121099224116 26.231195269508152 30.752265782547767 21.57541784871996 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13101.0 1 7970.5 2 2127.0 3 1268.0 4 1061.0 5 918.5 6 999.0 7 1238.0 8 1211.5 9 1210.5 10 1592.5 11 10606.0 12 193465.0 13 218410.0 14 61656.0 15 54810.5 16 57075.5 17 59989.0 18 62907.0 19 63832.0 20 63702.5 21 64267.5 22 65541.5 23 68219.0 24 73021.0 25 79638.5 26 86824.5 27 94274.5 28 101381.0 29 109460.0 30 118397.0 31 127428.0 32 136959.0 33 148604.0 34 168160.5 35 183526.0 36 192878.5 37 203111.5 38 212194.5 39 223234.0 40 234798.0 41 244685.0 42 251079.5 43 259953.0 44 271942.0 45 279385.5 46 281911.0 47 287332.0 48 294782.5 49 298263.5 50 295692.0 51 294503.0 52 297798.5 53 293967.5 54 272251.0 55 244687.0 56 229062.0 57 215748.0 58 218612.0 59 190499.5 60 136543.0 61 106802.0 62 78869.0 63 57900.5 64 42684.0 65 30780.5 66 21086.0 67 13952.5 68 9570.5 69 6753.0 70 5158.5 71 4444.0 72 3998.5 73 3659.0 74 3460.0 75 3257.0 76 3094.0 77 2931.0 78 2783.0 79 2646.5 80 2492.0 81 2279.5 82 2101.0 83 1958.0 84 1750.0 85 1508.0 86 1283.0 87 1100.5 88 905.5 89 698.0 90 555.0 91 432.5 92 304.0 93 207.5 94 136.5 95 86.5 96 55.5 97 40.0 98 25.0 99 19.0 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12069994156364489 2 0.10920731259250965 3 0.10436313120886273 4 0.09213786080950098 5 0.09734289543391618 6 0.08696563102515563 7 0.09592135236422294 8 0.0917551376753528 9 0.08691095629170588 10-11 0.07755610939845546 12-13 0.06892296898674159 14-15 0.07702029701064801 16-17 0.06746862107697853 18-19 0.060257023734957886 20-21 0.05644619481351105 22-23 0.049196325158075586 24-25 0.04656647047914312 26-27 0.04378899401989635 28-29 0.04607986535144044 30-31 0.03773650102701019 32-33 0.04974307249257299 34-35 0.05910338685916838 36-37 0.04980868217271267 38-39 0.05185351720373292 40-41 0.05224170781122608 42-43 0.0474740710544088 44-45 0.04756701810127335 46-47 0.04645712101224364 48-49 0.04671955973280239 50-51 0.04800441596887127 52-53 0.04459271260160752 54-55 0.04502464299586047 56-57 0.04544563844342346 58-59 0.04779118450841729 60-61 0.04387647359341594 62-63 0.05270097557220389 64-65 0.048425411416434264 66-67 0.043608567399512216 68-69 0.041700419202116305 70-71 0.049393154198494646 72-73 0.04351562035264766 74-75 0.045910373677746245 76-77 0.041295826174588234 78-79 0.04628762933854945 80-81 0.04785679418855697 82-83 0.03801534216760386 84-85 0.04799348102218132 86-87 0.05037183192724498 88-89 0.045828361577571634 90-91 0.042241699063268724 92-93 0.03982507584479024 94-95 0.04319303942529419 96-97 0.031093520912866848 98-99 0.03180975992105844 100-101 0.02383271631074144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9144992.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.35516565565552 #Duplication Level Percentage of deduplicated Percentage of total 1 91.68005629774744 47.082444784906436 2 4.787501686388521 4.917258843624253 3 1.2626150035202053 1.9452540799528864 4 0.5944907386089981 1.221206814480724 5 0.33435718053427005 0.8585484197247677 6 0.23596741493431425 0.7270887409973105 7 0.183774570751933 0.6606441466983753 8 0.1380645741492213 0.5672263261288635 9 0.1131141425241381 0.5228095974592085 >10 0.6396373139663778 5.298696256660269 >50 0.014085597798120702 0.5297858582792436 >100 0.00999496192956778 1.1285392940912733 >500 0.0027305876767801185 0.9933972861181278 >1k 0.0030545557054879415 3.1797222008050907 >5k 3.702491764227808E-4 1.305063919362002 >10k+ 1.851245882113904E-4 29.062313430711185 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1173357 12.830596243277196 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 28828 0.31523264317781796 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10464 0.1144232821636148 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 10113 0.11058511587544308 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 9628 0.10528166673081836 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0934946689947897E-5 0.0 2 0.0 0.0 0.0 2.1869893379895794E-5 0.0 3 0.0 0.0 0.0 3.280484006984369E-5 0.0 4 0.0 0.0 0.0 3.280484006984369E-5 0.0 5 0.0 0.0 0.0 3.280484006984369E-5 0.0 6 0.0 0.0 0.0 3.280484006984369E-5 0.0 7 0.0 0.0 0.0 4.373978675979159E-5 0.0 8 0.0 0.0 0.0 4.373978675979159E-5 0.0 9 0.0 0.0 0.0 4.373978675979159E-5 0.0 10-11 0.0 0.0 0.0 7.107715348466132E-5 0.0 12-13 0.0 0.0 0.0 9.841452020953107E-5 1.0934946689947897E-5 14-15 0.0 0.0 0.0 1.2028441358942687E-4 1.0934946689947897E-5 16-17 0.0 0.0 0.0 1.5308925365927056E-4 1.0934946689947897E-5 18-19 0.0 0.0 0.0 1.6402420034921844E-4 1.0934946689947897E-5 20-21 0.0 0.0 0.0 1.7495914703916635E-4 1.0934946689947897E-5 22-23 0.0 0.0 0.0 1.7495914703916635E-4 1.6402420034921846E-5 24-25 1.0934946689947897E-5 0.0 0.0 1.7495914703916635E-4 2.1869893379895794E-5 26-27 2.1869893379895794E-5 0.0 0.0 1.8589409372911424E-4 2.1869893379895794E-5 28-29 3.280484006984369E-5 0.0 0.0 1.9682904041906215E-4 2.1869893379895794E-5 30-31 3.827231341481764E-5 0.0 0.0 2.13231460453984E-4 2.1869893379895794E-5 32-33 4.373978675979159E-5 0.0 0.0 2.4603630052382765E-4 2.1869893379895794E-5 34-35 4.373978675979159E-5 0.0 0.0 2.952435606285932E-4 2.1869893379895794E-5 36-37 5.4674733449739486E-5 0.0 0.0 3.1164598066351506E-4 2.1869893379895794E-5 38-39 7.654462682963528E-5 0.0 0.0 3.17113454008489E-4 2.1869893379895794E-5 40-41 8.747957351958318E-5 0.0 0.0 3.280484006984369E-4 3.280484006984369E-5 42-43 1.0934946689947897E-4 0.0 0.0 3.4445082073335877E-4 3.280484006984369E-5 44-45 1.3121936027937477E-4 0.0 0.0 3.5538576742330665E-4 3.280484006984369E-5 46-47 1.585567270042445E-4 0.0 0.0 3.608532407682806E-4 3.280484006984369E-5 48-49 1.694916736941924E-4 0.0 0.0 3.6632071411325454E-4 3.280484006984369E-5 50-51 2.0776398710901006E-4 0.0 0.0 3.8819060749315036E-4 3.280484006984369E-5 52-53 2.569712472137756E-4 0.0 0.0 4.26462920907968E-4 3.280484006984369E-5 54-55 3.2258092735346295E-4 0.0 0.0 4.5926776097781165E-4 3.280484006984369E-5 56-57 3.7725566080320247E-4 0.0 0.0 4.7567018101273353E-4 3.280484006984369E-5 58-59 4.6473523432278565E-4 0.0 0.0 5.303449144624731E-4 3.280484006984369E-5 60-61 5.686172278772906E-4 0.0 0.0 5.467473344973948E-4 3.280484006984369E-5 62-63 6.779666947767697E-4 0.0 0.0 5.522148078423688E-4 3.280484006984369E-5 64-65 7.982511083661965E-4 0.0 0.0 5.686172278772906E-4 3.280484006984369E-5 66-67 9.62275308715415E-4 0.0 0.0 5.686172278772906E-4 3.280484006984369E-5 68-69 0.0011536368757895032 0.0 0.0 5.740847012222646E-4 4.373978675979159E-5 70-71 0.0014215430696932266 0.0 0.0 5.850196479122126E-4 4.373978675979159E-5 72-73 0.0016457094768371585 0.0 0.0 6.014220679471344E-4 4.373978675979159E-5 74-75 0.001930018090775804 0.0 0.0 6.014220679471344E-4 4.373978675979159E-5 76-77 0.0022471315447842926 0.0 0.0 6.178244879820561E-4 4.373978675979159E-5 78-79 0.0025423751054128862 0.0 0.0 6.287594346720041E-4 4.373978675979159E-5 80-81 0.0029524356062859323 0.0 5.467473344973949E-6 6.451618547069259E-4 4.373978675979159E-5 82-83 0.003521052834163223 0.0 1.0934946689947897E-5 6.560968013968738E-4 4.373978675979159E-5 84-85 0.004215421948974915 0.0 1.0934946689947897E-5 6.615642747418478E-4 4.373978675979159E-5 86-87 0.005183164731035304 0.0 1.0934946689947897E-5 6.889016414667175E-4 4.373978675979159E-5 88-89 0.006670317480868217 0.0 1.0934946689947897E-5 6.998365881566654E-4 4.373978675979159E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 256245 0.0 91.90329 5 AAGCAGT 258695 0.0 91.64785 1 GTATCAA 258615 0.0 91.45502 9 GGTATCA 258430 0.0 91.448944 8 TGGTATC 258255 0.0 91.27723 7 GTGGTAT 260090 0.0 90.73938 6 AGCAGTG 261195 0.0 90.672714 2 GCAGTGG 260755 0.0 90.63882 3 CAGTGGT 261075 0.0 90.28496 4 TCAACGC 257860 0.0 46.4227 12-13 AACGCAG 258840 0.0 46.294796 14-15 CAACGCA 257870 0.0 46.261528 12-13 ATCAACG 257780 0.0 46.249004 10-11 ACGCAGA 259235 0.0 46.05747 14-15 CGCAGAG 260230 0.0 46.03914 16-17 TATCAAC 259825 0.0 45.94717 10-11 CAGAGTA 258540 0.0 45.85395 18-19 AGAGTAC 253795 0.0 45.677143 18-19 GCAGAGT 261470 0.0 45.49104 16-17 GAGTACT 149885 0.0 45.4849 20-21 >>END_MODULE