FastQCFastQC Report
Sat 18 Jun 2016
SRR578558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9144992
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1442931.5778362627326519No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153030.16733748919627267No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105950.11585576017999798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4250.041.3321273
CGTACGC2950.028.96909720-21
CTCGCGT3300.026.61909552-53
ACGTACG3350.026.21871420-21
TCGCGTA3500.025.77632752-53
CGCGTAG3550.024.74207554-55
ACTCGCG4050.021.69028750-51
CGTAGCG4200.021.47716356-57
CGCTAGT4450.019.73768290-91
CAACGCA68200.019.6307712-13
AGTGGTA85700.019.2787155
ATCAACG69350.019.27111810-11
GTATCAA93050.017.9598889
AAGCAGT98700.017.77791
GGTATCA92950.017.7238248
ACGTGTA11100.017.1085957
TCAACGC65850.016.29399710-11
TGGTATC105300.015.6901897
GTCACGT11500.015.27508454
ACGCTAG5950.015.16076490-91
Produced by FastQC (version 0.11.5)