FastQCFastQC Report
Sat 18 Jun 2016
SRR578557_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578557_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8035212
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT99878112.430051627760411No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT245270.3052439686718907No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG94830.11801804358117746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88480.11011532738650828No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA83960.10449008688258629No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT81370.10126677429294959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2241750.091.7923055
AAGCAGT2254300.091.508471
GGTATCA2260750.091.297358
GTATCAA2259500.091.28759
TGGTATC2265100.091.059147
GTGGTAT2279200.090.5727166
GCAGTGG2276200.090.5452963
AGCAGTG2284500.090.387972
CAGTGGT2282450.090.142624
TCAACGC2247650.046.39100612-13
AACGCAG2254000.046.22043214-15
CAACGCA2246300.046.19909712-13
ATCAACG2248500.046.1837410-11
CGCAGAG2264400.046.0234316-17
ACGCAGA2256800.045.98850614-15
TATCAAC2270100.045.8833710-11
CAGAGTA2260250.045.67450718-19
AGAGTAC2220550.045.5271418-19
GAGTACT1265600.045.38460520-21
GCAGAGT2284950.045.31247716-17
Produced by FastQC (version 0.11.5)