##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR578557_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8035212
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87395180114725	31.0	31.0	34.0	27.0	34.0
2	31.230972997352154	33.0	31.0	34.0	28.0	34.0
3	31.35954234934934	34.0	31.0	34.0	28.0	34.0
4	34.72653963579306	37.0	35.0	37.0	32.0	37.0
5	34.74838386342513	37.0	35.0	37.0	32.0	37.0
6	34.786641472558536	37.0	35.0	37.0	32.0	37.0
7	34.71221705164717	37.0	35.0	37.0	32.0	37.0
8	34.751114469661786	37.0	35.0	37.0	32.0	37.0
9	36.24391179722451	39.0	37.0	39.0	32.0	39.0
10-11	36.12430885457658	39.0	37.0	39.0	32.0	39.0
12-13	36.19062340856719	39.0	37.0	39.0	32.0	39.0
14-15	37.44373098058894	40.0	38.0	41.0	32.0	41.0
16-17	37.41384421468905	40.0	38.0	41.0	31.5	41.0
18-19	37.333544404304455	40.0	38.0	41.0	31.0	41.0
20-21	37.256094923195555	40.0	37.5	41.0	31.0	41.0
22-23	36.9942194431211	39.5	37.0	41.0	30.5	41.0
24-25	36.95640787075686	40.0	37.0	41.0	31.0	41.0
26-27	36.84971068591594	40.0	37.0	41.0	30.0	41.0
28-29	36.69039777668591	40.0	36.5	41.0	30.0	41.0
30-31	36.45852530088814	40.0	36.0	41.0	29.5	41.0
32-33	36.16471780707217	39.5	36.0	41.0	27.5	41.0
34-35	35.99935956885767	39.0	35.5	41.0	27.0	41.0
36-37	35.714542752574545	39.0	35.0	40.5	27.0	41.0
38-39	34.83182372786182	38.0	34.5	40.0	25.5	41.0
40-41	34.604653803782654	38.0	34.0	40.0	25.0	41.0
42-43	34.300463883218015	37.0	34.0	40.0	24.0	41.0
44-45	34.02008391315624	37.0	33.0	40.0	23.5	41.0
46-47	33.86446151016302	37.0	33.0	40.0	23.0	41.0
48-49	33.8888309356368	37.0	33.0	40.0	22.5	41.0
50-51	33.01654063389989	37.0	32.0	39.5	17.5	40.5
52-53	32.597250514361036	37.0	33.0	39.5	9.5	40.5
54-55	32.20263796400145	38.0	31.5	40.0	2.0	41.0
56-57	31.697294358879393	37.5	31.0	40.0	2.0	41.0
58-59	31.41203113247043	37.0	31.0	40.0	2.0	41.0
60-61	31.14058851216371	37.0	31.0	40.0	2.0	41.0
62-63	30.80846516557373	36.0	30.0	40.0	2.0	41.0
64-65	30.511172200559237	36.0	30.0	39.5	2.0	41.0
66-67	30.173352924602362	35.0	29.0	39.0	2.0	41.0
68-69	29.838644456425044	35.0	29.0	39.0	2.0	41.0
70-71	29.540350522176638	35.0	29.0	38.0	2.0	40.0
72-73	29.215380440491177	35.0	29.0	37.0	2.0	40.0
74-75	28.84945026963819	35.0	28.0	37.0	2.0	39.0
76-77	28.477307817142844	35.0	27.5	36.0	2.0	39.0
78-79	28.1302772098608	35.0	27.0	36.0	2.0	38.5
80-81	27.808585013064995	34.5	26.0	35.5	2.0	37.0
82-83	27.543785901355186	34.0	26.0	35.0	2.0	37.0
84-85	27.279009875532843	34.0	26.0	35.0	2.0	36.5
86-87	27.038012811609697	34.0	25.5	35.0	2.0	36.0
88-89	26.776979188601373	34.0	25.0	35.0	2.0	36.0
90-91	26.544724893381783	34.0	24.5	35.0	2.0	35.5
92-93	26.271712619405687	34.0	23.5	35.0	2.0	35.0
94-95	25.98648305732319	34.0	21.5	35.0	2.0	35.0
96-97	25.710232287586187	34.0	19.5	35.0	2.0	35.0
98-99	25.39634330743234	34.0	9.5	35.0	2.0	35.0
100-101	24.553748861884415	33.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5402867581125648
1101	2	0.5906365193467416
1101	3	0.4672924861015879
1101	4	0.5148450241388005
1101	5	0.4554996225537593
1101	6	0.4849750155645296
1101	7	0.4416401372933123
1101	8	0.43529480878601134
1101	9	0.5418889175461601
1101	10-11	0.6054603580280826
1101	12-13	0.6356897649685607
1101	14-15	0.7744951390814165
1101	16-17	0.7325676602648556
1101	18-19	0.7730758233898669
1101	20-21	0.8121822788138076
1101	22-23	0.8346402397671753
1101	24-25	0.8096948638734673
1101	26-27	0.8619978388282235
1101	28-29	0.9521444057706177
1101	30-31	1.0046541739795103
1101	32-33	1.0565035628377828
1101	34-35	1.129579074855215
1101	36-37	1.0651345925202236
1101	38-39	1.0779348458142266
1101	40-41	1.0918751686823
1101	42-43	1.1114566304043336
1101	44-45	1.2360222514184827
1101	46-47	1.14324891855167
1101	48-49	1.1003905027856646
1101	50-51	1.253273159436766
1101	52-53	1.003891852969339
1101	54-55	0.9523860150896297
1101	56-57	0.9763504815003756
1101	58-59	0.9647051319647488
1101	60-61	0.9461831726590013
1101	62-63	0.9430488099229031
1101	64-65	0.9963657344906345
1101	66-67	1.0071536261275078
1101	68-69	0.8843562751949001
1101	70-71	0.9936559681562755
1101	72-73	0.905677344223971
1101	74-75	0.9200630487644688
1101	76-77	0.9381410123488898
1101	78-79	0.9572292535308584
1101	80-81	0.9107815603475196
1101	82-83	0.9860535422507759
1101	84-85	0.9512203780201354
1101	86-87	0.9620647995266154
1101	88-89	0.9388036119678773
1101	90-91	0.9937879008605925
1101	92-93	1.0549044522486462
1101	94-95	1.1131970863045844
1101	96-97	1.1142255907004355
1101	98-99	1.0635846737927714
1101	100-101	1.2432722727129821
1102	1	0.5223824478151116
1102	2	0.46876072801389057
1102	3	0.5370500071142992
1102	4	0.4936155810600553
1102	5	0.5574254618194558
1102	6	0.5540793758904172
1102	7	0.5886918049059986
1102	8	0.5158915565460944
1102	9	0.6329237014528246
1102	10-11	0.6264427831870663
1102	12-13	0.6250657336927503
1102	14-15	0.7853695226375734
1102	16-17	0.7771018003394801
1102	18-19	0.8624787687089324
1102	20-21	0.8854195486096188
1102	22-23	0.8475891145606127
1102	24-25	0.9105053080505527
1102	26-27	0.9316293453832643
1102	28-29	1.010444007014712
1102	30-31	1.0384817683845498
1102	32-33	1.1134467117420215
1102	34-35	1.061065478341284
1102	36-37	1.1152531247315949
1102	38-39	1.1093831417820113
1102	40-41	1.1411168333964596
1102	42-43	1.1947740756250766
1102	44-45	1.23704445879779
1102	46-47	1.2926960563554601
1102	48-49	1.2463161293078144
1102	50-51	1.2036811830605245
1102	52-53	0.9854101268074658
1102	54-55	0.969038759382677
1102	56-57	0.9331683962755619
1102	58-59	0.929011331452493
1102	60-61	0.9339172156720146
1102	62-63	0.9494756691247517
1102	64-65	0.9173205380335148
1102	66-67	0.8712658221377225
1102	68-69	0.8796147367158049
1102	70-71	0.9248595245789666
1102	72-73	0.8821632170277489
1102	74-75	0.8235650631762859
1102	76-77	0.9163384605507652
1102	78-79	0.9432237499155498
1102	80-81	0.9332193404017666
1102	82-83	0.9496180051391576
1102	84-85	0.9505533378210416
1102	86-87	0.9319991202290367
1102	88-89	0.9885912041646279
1102	90-91	0.9592117876785373
1102	92-93	0.9676779640478834
1102	94-95	1.1086618096479164
1102	96-97	1.1399360235999438
1102	98-99	1.223856511709382
1102	100-101	1.308151399389697
1103	1	0.3751461628668302
1103	2	0.42604573335734486
1103	3	0.3180866907173723
1103	4	0.404695975429469
1103	5	0.43013390517410244
1103	6	0.45875090167384514
1103	7	0.3486404483663108
1103	8	0.3920910597171954
1103	9	0.500885204491567
1103	10-11	0.5107319356343396
1103	12-13	0.5629925929178157
1103	14-15	0.5952625028365475
1103	16-17	0.7192662873469544
1103	18-19	0.7057847391242049
1103	20-21	0.6960423994943739
1103	22-23	0.8161545751446724
1103	24-25	0.7350601155798131
1103	26-27	0.747999641088839
1103	28-29	0.8324293095426611
1103	30-31	0.8815253009818846
1103	32-33	0.8654249320677465
1103	34-35	0.8765589009836177
1103	36-37	0.9656753489906151
1103	38-39	0.8179552852406786
1103	40-41	0.9376510331947046
1103	42-43	0.8333019415115501
1103	44-45	0.8229982946977827
1103	46-47	1.0538976508614084
1103	48-49	1.056763204877548
1103	50-51	0.9390479729963488
1103	52-53	0.8434810700351534
1103	54-55	0.8009319538179298
1103	56-57	0.7797172841813023
1103	58-59	0.765507882533143
1103	60-61	0.835222871816935
1103	62-63	0.7283866225761209
1103	64-65	0.644597062072453
1103	66-67	0.6345731217850776
1103	68-69	0.7908194276685769
1103	70-71	0.7393483811065238
1103	72-73	0.8381309628396956
1103	74-75	0.7972591947520975
1103	76-77	0.799756710036295
1103	78-79	0.8296640110056153
1103	80-81	0.8543039994413242
1103	82-83	0.8881263275860007
1103	84-85	0.9281776763750358
1103	86-87	0.9181509026987733
1103	88-89	0.9588300055561838
1103	90-91	0.9538715672619844
1103	92-93	0.9567572210694983
1103	94-95	0.9107183290780121
1103	96-97	0.9483795205699401
1103	98-99	1.0893757034366445
1103	100-101	1.1740757372061736
1104	1	0.3535616109669739
1104	2	0.31934172583625653
1104	3	0.35827601856715674
1104	4	0.366564585867998
1104	5	0.46026499009394684
1104	6	0.43329057630076306
1104	7	0.47254953707241754
1104	8	0.34863833258114596
1104	9	0.5060529848805544
1104	10-11	0.487307664796063
1104	12-13	0.5268711704787776
1104	14-15	0.638583649268277
1104	16-17	0.5913685754726572
1104	18-19	0.6454074323045873
1104	20-21	0.6867143259273831
1104	22-23	0.6802957515658008
1104	24-25	0.7168887801317894
1104	26-27	0.7164364685034244
1104	28-29	0.754671295143396
1104	30-31	0.8437798753045413
1104	32-33	0.8469123584295346
1104	34-35	0.8696705259302036
1104	36-37	0.8618117475825358
1104	38-39	0.8702061776527188
1104	40-41	0.8221844678609429
1104	42-43	0.9428647770624394
1104	44-45	0.9608511980413255
1104	46-47	1.058849375548462
1104	48-49	0.9428247313596145
1104	50-51	0.9513332278468738
1104	52-53	0.8067368569897582
1104	54-55	0.7986763508352084
1104	56-57	0.7605730180709074
1104	58-59	0.782492333446271
1104	60-61	0.8256105258192115
1104	62-63	0.658487781153962
1104	64-65	0.5447729295592794
1104	66-67	0.7237665410313063
1104	68-69	0.7171811535029775
1104	70-71	0.6418660265827896
1104	72-73	0.6468989483177872
1104	74-75	0.7097395735561598
1104	76-77	0.6712087292267412
1104	78-79	0.6506644892336304
1104	80-81	0.7315830934588767
1104	82-83	0.7333044279575063
1104	84-85	0.7630591616315705
1104	86-87	0.7314795180432121
1104	88-89	0.7585366025311338
1104	90-91	0.7663709490768547
1104	92-93	0.6506220242294631
1104	94-95	0.548208326842655
1104	96-97	0.6705795808553532
1104	98-99	0.978258781385339
1104	100-101	0.975734902814505
1105	1	0.2519281431068805
1105	2	0.18188475549017724
1105	3	0.2038622339032088
1105	4	0.23224540876456246
1105	5	0.17698929919343698
1105	6	0.16962288593683184
1105	7	0.18571045388559781
1105	8	0.19868993367791177
1105	9	0.12717089130548942
1105	10-11	0.17615816503771242
1105	12-13	0.1524314508824034
1105	14-15	0.06064001570670996
1105	16-17	0.0852089472598081
1105	18-19	-0.044467501038546686
1105	20-21	0.056778841271899694
1105	22-23	0.022935485111965193
1105	24-25	0.005826417429481978
1105	26-27	-0.2609163405275652
1105	28-29	-0.055115403178447764
1105	30-31	-0.2954677996729984
1105	32-33	-0.27690680862482253
1105	34-35	-0.180560849510762
1105	36-37	-0.10111868720576211
1105	38-39	-0.08812035471789414
1105	40-41	-0.04164025710366559
1105	42-43	0.11267492302255278
1105	44-45	0.08681802736025901
1105	46-47	0.1775414566174831
1105	48-49	0.19547888324402862
1105	50-51	0.10530222211664864
1105	52-53	-0.026707722254968758
1105	54-55	-0.020009247392628993
1105	56-57	0.12437343052290828
1105	58-59	0.16667250333149752
1105	60-61	0.1697111879163451
1105	62-63	0.22961164715475135
1105	64-65	0.0919306935204709
1105	66-67	0.06688798342169733
1105	68-69	0.0978082923500061
1105	70-71	0.13334691027327494
1105	72-73	0.12991141446013899
1105	74-75	0.1489640747178136
1105	76-77	0.17294278146717446
1105	78-79	0.17793096840481937
1105	80-81	0.26377384870437126
1105	82-83	0.24426889544703556
1105	84-85	0.16175327719186328
1105	86-87	0.1781091303892346
1105	88-89	0.25100727529336453
1105	90-91	0.25870228758005354
1105	92-93	0.3389814949632317
1105	94-95	0.345865028753618
1105	96-97	0.3427503818532678
1105	98-99	0.39800102920950664
1105	100-101	0.37046684439288313
1106	1	-0.09992965637657392
1106	2	-0.04149966069540767
1106	3	-0.08491790681967615
1106	4	-0.0517039394151837
1106	5	-0.11026459697036017
1106	6	-0.08551101724513188
1106	7	-0.1078375101376281
1106	8	-0.14511990209221182
1106	9	-0.13181398494698016
1106	10-11	-0.22191001781855846
1106	12-13	-0.24714900095852954
1106	14-15	-0.2879057680056363
1106	16-17	-0.2591246609725175
1106	18-19	-0.2961478272320264
1106	20-21	-0.13384392150776847
1106	22-23	-0.12091403805585088
1106	24-25	-0.22416595956666896
1106	26-27	-0.16794588806495625
1106	28-29	-0.22096219418040874
1106	30-31	-0.3065738205027131
1106	32-33	-0.21455399005091635
1106	34-35	-0.13093668366291666
1106	36-37	-0.20940883492498585
1106	38-39	-0.22800657137785407
1106	40-41	-0.3102003218387708
1106	42-43	-0.35656377660890115
1106	44-45	-0.2871236723503827
1106	46-47	-0.36962219297338805
1106	48-49	-0.5875404071312076
1106	50-51	-0.6302348705602014
1106	52-53	-0.6558383819718756
1106	54-55	-0.6542928587063912
1106	56-57	-0.6458248450508677
1106	58-59	-0.5534307753887902
1106	60-61	-0.7801234118845066
1106	62-63	-0.6524577535065994
1106	64-65	-0.6274956809976509
1106	66-67	-0.8026795905455053
1106	68-69	-0.9800507376307372
1106	70-71	-0.8303620496726261
1106	72-73	-0.8661588386000894
1106	74-75	-0.9002200980091608
1106	76-77	-0.9243689243284514
1106	78-79	-0.8838418655145368
1106	80-81	-0.84257196953833
1106	82-83	-1.0182384756663723
1106	84-85	-1.0413006832521639
1106	86-87	-0.9561709306098898
1106	88-89	-1.0436488348978408
1106	90-91	-0.9454430948268318
1106	92-93	-0.923654997792628
1106	94-95	-0.9317243888511513
1106	96-97	-0.9115571637011755
1106	98-99	-0.9276424158034757
1106	100-101	-1.037141067589065
1107	1	-0.37844930451951697
1107	2	-0.45102814090348886
1107	3	-0.4080511463912373
1107	4	-0.2555718537770204
1107	5	-0.31232921903677635
1107	6	-0.3350352915456867
1107	7	-0.2949640470140267
1107	8	-0.2726723441803287
1107	9	-0.3115974548484317
1107	10-11	-0.3361343904301748
1107	12-13	-0.3969213501595519
1107	14-15	-0.6519732242687937
1107	16-17	-0.490835099680595
1107	18-19	-0.43847372233496884
1107	20-21	-0.37222591954969886
1107	22-23	-0.42286268178570197
1107	24-25	-0.41835453885605745
1107	26-27	-0.3653205556472372
1107	28-29	-0.3967432512315128
1107	30-31	-0.40603601421837965
1107	32-33	-0.34850890220943853
1107	34-35	-0.4044485667080906
1107	36-37	-0.5347210190084866
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2108	10-11	-1.7511031388887872
2108	12-13	-1.2430774283566208
2108	14-15	-1.2601500478995007
2108	16-17	-1.26210055697549
2108	18-19	-1.2171055230638999
2108	20-21	-1.1879465703255931
2108	22-23	-1.1626180288098809
2108	24-25	-1.3073356949151744
2108	26-27	-1.4597167636087462
2108	28-29	-1.6799823807954226
2108	30-31	-1.8272674113317535
2108	32-33	-1.7753893014254558
2108	34-35	-2.482532320364669
2108	36-37	-2.2985321196704263
2108	38-39	-2.080681863445527
2108	40-41	-1.9635787913978717
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2108	54-55	-2.007508939038132
2108	56-57	-1.7553930246031655
2108	58-59	-1.5703923480126107
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2108	62-63	-1.3372558986944085
2108	64-65	-1.4979106730722584
2108	66-67	-1.3815159051824395
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2108	76-77	-1.3566532980586743
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2108	80-81	-1.2478690124577447
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2201	78-79	1.1763556696932227
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2202	9	0.6136834046126296
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2202	28-29	1.0954079819425573
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2202	34-35	1.1645553112237081
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2202	68-69	1.2190224645839258
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2202	76-77	1.2450883518180014
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2203	9	0.6655590193987209
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2206	1	0.047415642762697274
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2207	4	-0.31682061652477955
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2207	9	-0.4046324846895999
2207	10-11	-0.3888636991483949
2207	12-13	-0.40250501664783656
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2207	22-23	-0.3567003291923001
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2207	26-27	-0.4286800324581037
2207	28-29	-0.4392762863200943
2207	30-31	-0.5455356833936946
2207	32-33	-0.5323538778667896
2207	34-35	-0.4850817611357314
2207	36-37	-0.5846343413524551
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2207	40-41	-0.6600336622225171
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2207	68-69	-0.5443420407838424
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2207	82-83	-0.5704743475484761
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2207	86-87	-0.6238011265714682
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2207	92-93	-0.610764632023983
2207	94-95	-0.49516852871999717
2207	96-97	-0.5402981625893446
2207	98-99	-0.608917320228759
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2208	2	-0.7336780591825622
2208	3	-0.7025326154534604
2208	4	-0.6790268680675311
2208	5	-0.6329071584905748
2208	6	-0.7027448516161101
2208	7	-0.6363108939126931
2208	8	-0.6147141260999192
2208	9	-0.8297988601529909
2208	10-11	-0.9424322509296843
2208	12-13	-0.9093030951896779
2208	14-15	-1.264552409171415
2208	16-17	-1.1671461034874113
2208	18-19	-1.3431381335807018
2208	20-21	-1.230233915461099
2208	22-23	-1.0523818099012203
2208	24-25	-1.1264447705350378
2208	26-27	-1.085802833574796
2208	28-29	-1.094198506750729
2208	30-31	-1.5533015275959627
2208	32-33	-1.446155789684525
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2308	58-59	-1.9487970229186864
2308	60-61	-1.9094309476043172
2308	62-63	-1.9674243849169777
2308	64-65	-1.9123560343867219
2308	66-67	-2.0735376248968507
2308	68-69	-1.8729344908213363
2308	70-71	-1.8241898933037746
2308	72-73	-1.8442490236139584
2308	74-75	-1.6674573978192377
2308	76-77	-1.593511120535954
2308	78-79	-1.7028365040607092
2308	80-81	-2.2962130701109764
2308	82-83	-2.063727735947136
2308	84-85	-1.791616260672381
2308	86-87	-1.7228165107487818
2308	88-89	-1.5811468295162108
2308	90-91	-1.780642705950914
2308	92-93	-1.8689039485097148
2308	94-95	-1.9396569580740426
2308	96-97	-1.8300940468722047
2308	98-99	-1.878975958561096
2308	100-101	-2.0623825059304224
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	112586.0
3	16219.0
4	13656.0
5	12397.0
6	13737.0
7	16453.0
8	19518.0
9	22377.0
10	25135.0
11	29642.0
12	29491.0
13	32015.0
14	35364.0
15	41678.0
16	52250.0
17	71669.0
18	110885.0
19	180384.0
20	261746.0
21	186485.0
22	101799.0
23	86711.0
24	89100.0
25	92347.0
26	97925.0
27	109780.0
28	125219.0
29	146001.0
30	171096.0
31	205124.0
32	251630.0
33	318291.0
34	419454.0
35	615463.0
36	1013936.0
37	1362589.0
38	1282286.0
39	262346.0
40	428.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.614358400823592	47.65877502861044	24.414209704975335	17.312656865590633
2	16.372012177067486	49.30644801857328	18.35313371144077	15.968406092918464
3	44.17498903615708	20.503202089812465	19.163178227807045	16.15863064622341
4	16.405771475710928	18.93045278378757	20.147941538126293	44.51583420237521
5	16.444158409354827	47.007631897252566	21.02438039489367	15.523829298498942
6	44.51245571620337	20.05275350443417	18.9257864177523	16.509004361610163
7	17.227899669276297	19.539841789268245	47.89944277026339	15.332815771192065
8	44.07741661193608	20.24848618132121	19.804953768059068	15.869143438683638
9	43.675030425322234	19.888365391628472	20.387442737041823	16.049161446007467
10-11	16.07313273808905	33.639033235132736	33.88155898797478	16.406275038803436
12-13	15.91355880441356	20.205359049414966	33.822815427483135	30.058266718688337
14-15	16.079136417536265	47.692847113477754	19.802593968599197	16.425422500386784
16-17	29.922988335588137	20.029722162743465	19.94060670075344	30.106682800914964
18-19	16.213588239638995	33.71699498510391	19.940993096405094	30.128423678852005
20-21	30.0583270671592	33.76563712243345	19.961236836989617	16.214798973417732
22-23	29.97120411550413	19.95017284002242	33.83029195626829	16.248331088205163
24-25	16.15940950390174	33.559058446011285	20.409521076292116	29.87201097379486
26-27	16.333508170630832	25.57926081594984	41.601440780466724	16.485790232952603
28-29	27.818586644960202	19.81257451304351	35.98877871445441	16.380060127541878
30-31	25.77615907359242	20.943550460732048	36.374103869382544	16.906186596292986
32-33	19.791680278654027	23.65815673218715	37.685734400786934	18.864428588371883
34-35	19.35767950982403	22.800644978862454	38.335053725006816	19.506621786306695
36-37	19.116694171759843	22.640125542948425	38.62751151078971	19.615668774502023
38-39	19.24007996538136	22.766879108676978	38.467216520968265	19.525824404973406
40-41	19.405056436588815	22.460627307186968	38.25529278537867	19.879023470845546
42-43	19.907166134014528	22.762946576999322	38.2353239499688	19.09456333901735
44-45	19.50556283502381	22.90687182010705	38.03696293905414	19.550602405814995
46-47	19.811560797880652	22.65579637569435	38.11490357041094	19.41773925601406
48-49	19.57348298170252	22.729109291477855	38.34262381762022	19.354783909199405
50-51	19.790681183802235	22.725575296932217	37.51066367263451	19.973079846631045
52-53	20.244464629091738	24.00257028180512	36.13876531019274	19.614199778910404
54-55	20.78192089128089	24.648815749584916	34.53619364260092	20.03306971653327
56-57	20.83093849455902	25.47763910609882	33.3124176292283	20.379004770113855
58-59	20.990572150263457	26.142683199129184	32.42607017757212	20.440674473035234
60-61	21.291249624060526	26.324845593839854	32.037748932979746	20.346155849119885
62-63	21.33653576304346	26.06276508894779	32.13412079630485	20.466578351703905
64-65	20.664711643415025	25.76007587349177	32.35883691130248	21.216375571790724
66-67	20.922082627961842	25.466012454079724	33.1205526706592	20.491352247299236
68-69	20.81828840354633	25.222536600709493	33.157536023711984	20.801638972032197
70-71	20.59331973268464	25.248928318101914	33.810284345765496	20.34746760344795
72-73	20.193219512432155	24.959459885439834	33.74721732529064	21.10010327683737
74-75	20.58261392459005	25.33327842551285	32.87744474786346	21.20666290203365
76-77	21.058466212505113	25.850503026445608	32.34878909463963	20.742241666409647
78-79	21.028751784051874	25.911116686386816	32.13710018402772	20.92303134553359
80-81	20.73721327859986	26.376437189849884	32.002170895051805	20.884178636498454
82-83	21.662920085562096	26.65078823979714	31.23717291238059	20.44911876226018
84-85	21.174587320325255	27.04926836720174	30.701906205166203	21.074238107306808
86-87	21.047814967106646	27.458101649816946	30.565003849338208	20.9290795337382
88-89	21.04664095801695	26.98058182687661	30.631757078990024	21.341020136116416
90-91	20.97474035356402	26.798577669004615	30.818270236658478	21.408411740772888
92-93	20.927901319657643	27.313744374715093	30.603469254154913	21.15488505147235
94-95	21.024799408529148	26.735574375321857	30.2473110244585	21.99231519169049
96-97	20.99437955264525	26.644361958754136	30.57827623742766	21.782982251172957
98-99	21.384081949659326	27.078146840597167	30.151016045766426	21.386755163977085
100-101	21.54597445230666	26.716591371299874	30.07796994162519	21.659464234768272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10161.0
1	5994.5
2	1389.5
3	821.5
4	639.0
5	599.5
6	744.5
7	906.5
8	881.0
9	959.0
10	1499.0
11	8465.0
12	148005.5
13	166459.5
14	47526.5
15	44186.0
16	46656.0
17	49803.0
18	53045.0
19	54564.0
20	55498.0
21	56080.0
22	57017.0
23	59566.0
24	63752.5
25	69727.5
26	75671.5
27	82019.0
28	89095.5
29	96331.5
30	103656.5
31	111145.0
32	120112.0
33	130693.5
34	147907.5
35	161218.5
36	169050.5
37	178487.5
38	186599.5
39	196549.5
40	206775.0
41	215338.0
42	221109.5
43	228964.0
44	240209.5
45	247277.5
46	250052.0
47	255548.0
48	262939.5
49	266552.0
50	264344.0
51	264217.5
52	268868.5
53	267631.0
54	247250.5
55	221677.0
56	209133.0
57	197460.0
58	203468.0
59	176580.0
60	123563.0
61	97201.0
62	70569.5
63	50900.5
64	37428.0
65	26169.0
66	17219.0
67	11089.0
68	7145.0
69	4721.5
70	3544.0
71	2841.5
72	2474.0
73	2210.0
74	1977.5
75	1794.5
76	1613.5
77	1432.5
78	1290.5
79	1134.0
80	936.0
81	750.5
82	631.5
83	572.0
84	489.0
85	394.0
86	305.0
87	249.5
88	201.0
89	140.0
90	117.5
91	97.5
92	65.0
93	49.0
94	35.0
95	22.5
96	18.5
97	12.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05044048619999074
2	0.0912857059651942
3	0.08150376119509976
4	0.05397492934847269
5	0.08302207832226455
6	0.001319193569503829
7	0.001319193569503829
8	0.004965643719170073
9	0.009781944770094428
10-11	0.010049517050701338
12-13	0.0171806294594343
14-15	0.011934968237303509
16-17	0.0016241015171721666
18-19	6.284837288673902E-4
20-21	7.467133412285824E-5
22-23	4.480280047371495E-4
24-25	8.711655647666794E-4
26-27	0.001524539738341689
28-29	0.0015618754054031183
30-31	0.004853636717985785
32-33	1.866783353071456E-4
34-35	0.0027379489178381354
36-37	4.1691494885262516E-4
38-39	0.002924627253145281
40-41	0.0032979839237595723
42-43	0.001312970958326924
44-45	6.907098406364388E-4
46-47	0.0012507448465578755
48-49	0.002470376637231227
50-51	0.004225152989118396
52-53	0.12956720992551285
54-55	0.14006475498095133
56-57	0.12621322250116113
58-59	0.11982260082247986
60-61	0.10770095424986921
62-63	0.1121874569084176
64-65	0.1147511727133024
66-67	0.12308324907917799
68-69	0.13582715676947915
70-71	0.12270366979738681
72-73	0.11463294310094121
74-75	0.11997194349072557
76-77	0.13110419488620836
78-79	0.14551576237192
80-81	0.1353044574306191
82-83	0.11484451188095597
84-85	0.12119157528139891
86-87	0.12204407301263488
88-89	0.1183353967511996
90-91	0.12889516791840713
92-93	0.14597623559901093
94-95	0.15557150203379824
96-97	0.15607553353912754
98-99	0.13893846235793156
100-101	0.1276942039612645
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	8035212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.95626870092269
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.45194921241429	49.3440594492816
2	5.178560583355251	5.588316126390458
3	1.2185147826736453	1.972395330899569
4	0.5651396953930884	1.219713170327483
5	0.349497355062562	0.942878660000869
6	0.216939748895836	0.7023155630000654
7	0.17648299943733242	0.6665654897149846
8	0.13191376961088008	0.5694059838781004
9	0.1111021003068721	0.539518929965501
>10	0.5700212391736738	4.836276500194849
>50	0.01477998075335095	0.5738281308653683
>100	0.00996260011515921	1.2687762543551164
>500	0.0019865400484370133	0.7332849292655871
>1k	0.002713322992836736	2.89172920932607
>5k	3.39165374104592E-4	1.3985980656757502
>10k+	9.6904392601312E-5	26.75233820685859
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	998781	12.430051627760411	No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT	24527	0.3052439686718907	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG	9483	0.11801804358117746	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8848	0.11011532738650828	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA	8396	0.10449008688258629	No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT	8137	0.10126677429294959	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2445222353809707E-5	0.0	0.0	0.0	0.0
2	1.2445222353809707E-5	0.0	0.0	3.733566706142912E-5	0.0
3	1.2445222353809707E-5	0.0	0.0	6.222611176904854E-5	0.0
4	2.4890444707619413E-5	0.0	0.0	7.467133412285824E-5	0.0
5	2.4890444707619413E-5	0.0	0.0	7.467133412285824E-5	0.0
6	2.4890444707619413E-5	0.0	0.0	1.1200700118428736E-4	0.0
7	3.733566706142912E-5	0.0	0.0	1.1200700118428736E-4	0.0
8	3.733566706142912E-5	0.0	0.0	1.1200700118428736E-4	0.0
9	3.733566706142912E-5	0.0	0.0	1.1200700118428736E-4	0.0
10-11	3.733566706142912E-5	0.0	0.0	1.3067483471500193E-4	0.0
12-13	3.733566706142912E-5	0.0	0.0	1.3689744589190679E-4	0.0
14-15	3.733566706142912E-5	0.0	0.0	1.3689744589190679E-4	0.0
16-17	4.3558278238333976E-5	0.0	0.0	1.3689744589190679E-4	6.222611176904853E-6
18-19	4.978088941523883E-5	0.0	0.0	1.3689744589190679E-4	1.2445222353809707E-5
20-21	4.978088941523883E-5	0.0	0.0	1.5556527942262133E-4	1.2445222353809707E-5
22-23	4.978088941523883E-5	0.0	0.0	1.9290094648405045E-4	1.2445222353809707E-5
24-25	4.978088941523883E-5	0.0	0.0	2.0534616883786016E-4	1.2445222353809707E-5
26-27	6.222611176904854E-5	0.0	0.0	2.1779139119166988E-4	1.2445222353809707E-5
28-29	6.222611176904854E-5	0.0	0.0	2.240140023685747E-4	1.2445222353809707E-5
30-31	6.844872294595338E-5	0.0	0.0	2.4268183589928928E-4	1.2445222353809707E-5
32-33	7.467133412285824E-5	0.0	0.0	2.6134966943000386E-4	1.2445222353809707E-5
34-35	8.711655647666795E-5	0.0	0.0	2.800175029607184E-4	1.2445222353809707E-5
36-37	8.711655647666795E-5	0.0	0.0	2.8624011413762323E-4	1.2445222353809707E-5
38-39	9.956177883047765E-5	0.0	0.0	2.9868533649143295E-4	1.2445222353809707E-5
40-41	1.057843900073825E-4	0.0	0.0	3.360210035528621E-4	1.2445222353809707E-5
42-43	1.3689744589190679E-4	0.0	0.0	3.5468883708357664E-4	1.2445222353809707E-5
44-45	1.3689744589190679E-4	0.0	0.0	3.982471153219106E-4	1.2445222353809707E-5
46-47	1.6801050177643102E-4	0.0	0.0	4.2313756002953004E-4	1.2445222353809707E-5
48-49	2.11568780014765E-4	0.0	0.0	4.293601712064349E-4	1.2445222353809707E-5
50-51	2.3645922472238443E-4	0.0	0.0	4.418053935602446E-4	1.2445222353809707E-5
52-53	2.862401141376233E-4	0.0	0.0	4.791410606216737E-4	1.2445222353809707E-5
54-55	3.42243614729767E-4	0.0	0.0	4.978088941523883E-4	1.2445222353809707E-5
56-57	3.858018929681009E-4	0.0	0.0	5.164767276831029E-4	1.2445222353809707E-5
58-59	4.293601712064349E-4	0.0	0.0	5.351445612138174E-4	1.2445222353809707E-5
60-61	5.662576170983417E-4	0.0	0.0	5.351445612138174E-4	1.2445222353809707E-5
62-63	6.098158953366757E-4	0.0	0.0	5.475897835676271E-4	1.2445222353809707E-5
64-65	6.969324518133436E-4	0.0	0.0	5.724802282752465E-4	1.2445222353809707E-5
66-67	7.964942306438212E-4	0.0	0.0	5.787028394521514E-4	2.4890444707619413E-5
68-69	9.022786206512037E-4	6.222611176904853E-6	0.0	5.973706729828659E-4	2.4890444707619413E-5
70-71	0.0010329534553662058	1.2445222353809707E-5	0.0	5.973706729828659E-4	2.4890444707619413E-5
72-73	0.0011947413459657318	1.2445222353809707E-5	0.0	6.471515623981048E-4	2.4890444707619413E-5
74-75	0.0014498684042188307	1.2445222353809707E-5	0.0	6.658193959288192E-4	2.4890444707619413E-5
76-77	0.001817002463656217	1.2445222353809707E-5	0.0	6.720420071057242E-4	2.4890444707619413E-5
78-79	0.002190359134270508	1.2445222353809707E-5	6.222611176904853E-6	6.844872294595338E-4	2.4890444707619413E-5
80-81	0.002632164527830753	1.2445222353809707E-5	1.2445222353809707E-5	6.844872294595338E-4	2.4890444707619413E-5
82-83	0.003123750810806236	1.2445222353809707E-5	1.2445222353809707E-5	6.844872294595338E-4	2.4890444707619413E-5
84-85	0.003932690263803868	1.2445222353809707E-5	1.2445222353809707E-5	6.907098406364388E-4	2.4890444707619413E-5
86-87	0.005139876832123409	1.2445222353809707E-5	1.2445222353809707E-5	7.093776741671533E-4	2.4890444707619413E-5
88-89	0.006776423571649386	2.4890444707619413E-5	1.2445222353809707E-5	7.093776741671533E-4	2.4890444707619413E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGTA	224175	0.0	91.792305	5
AAGCAGT	225430	0.0	91.50847	1
GGTATCA	226075	0.0	91.29735	8
GTATCAA	225950	0.0	91.2875	9
TGGTATC	226510	0.0	91.05914	7
GTGGTAT	227920	0.0	90.572716	6
GCAGTGG	227620	0.0	90.545296	3
AGCAGTG	228450	0.0	90.38797	2
CAGTGGT	228245	0.0	90.14262	4
TCAACGC	224765	0.0	46.391006	12-13
AACGCAG	225400	0.0	46.220432	14-15
CAACGCA	224630	0.0	46.199097	12-13
ATCAACG	224850	0.0	46.18374	10-11
CGCAGAG	226440	0.0	46.02343	16-17
ACGCAGA	225680	0.0	45.988506	14-15
TATCAAC	227010	0.0	45.88337	10-11
CAGAGTA	226025	0.0	45.674507	18-19
AGAGTAC	222055	0.0	45.52714	18-19
GAGTACT	126560	0.0	45.384605	20-21
GCAGAGT	228495	0.0	45.312477	16-17
>>END_MODULE