FastQCFastQC Report
Sat 18 Jun 2016
SRR578557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8035212
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1016121.264583933815312No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130550.16247237782898571No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96850.12053197849664701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG2900.029.4318733
CGTACGC1900.022.45879220-21
ACGTACG2100.020.31985720-21
CAACGCA58050.019.72245612-13
CTCGCGT2300.019.58131652-53
CGCGTAG2300.019.58131654-55
ATCAACG59900.019.15290510-11
AGTGGTA73100.017.9022675
TCAACGC57900.017.60378310-11
AAGCAGT84600.016.2007731
AACGCAG63300.016.17693112-13
GGTATCA81900.016.094298
GTATCAA81100.016.0765579
CGTAGCG2700.015.80246556-57
TCGCGTA2950.015.26678952-53
CGCTAGT2801.0913936E-1114.75149790-91
TGGTATC93000.014.2243437
ACTCGCG3003.6379788E-1214.222218550-51
ACGCAGA82100.014.11827914-15
TATGCCG6450.013.9649748-49
Produced by FastQC (version 0.11.5)