FastQCFastQC Report
Sat 18 Jun 2016
SRR578556_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578556_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10694765
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149064813.938108972006397No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT365530.34178404107056115No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC353710.3307319048151128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA142650.13338301496105806No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG128330.11999328643499879No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT116880.10928711383560087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA3287150.091.876115
GGTATCA3300950.091.618748
TGGTATC3301550.091.571817
GTATCAA3302300.091.541899
GTGGTAT3308800.091.442356
AAGCAGT3324650.091.282451
AGCAGTG3342000.090.771262
GCAGTGG3335900.090.735683
CAGTGGT3335950.090.454874
TCAACGC3291700.046.4566112-13
AACGCAG3301600.046.3346714-15
CAACGCA3292900.046.2809712-13
ATCAACG3295000.046.2668710-11
CGCAGAG3315650.046.1954616-17
ACGCAGA3307650.046.11993414-15
TATCAAC3318650.046.01374410-11
CAGAGTA3293950.045.86591718-19
AGAGTAC3236400.045.752518-19
GAGTACT1901100.045.61394520-21
GCAGAGT3335850.045.5419416-17
Produced by FastQC (version 0.11.5)