##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578556_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10694765 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.575960762111183 31.0 31.0 34.0 27.0 34.0 2 30.88087433431216 31.0 31.0 34.0 27.0 34.0 3 30.967018723646568 31.0 31.0 34.0 27.0 34.0 4 34.3579692494412 37.0 35.0 37.0 30.0 37.0 5 34.44641354905882 37.0 35.0 37.0 30.0 37.0 6 34.4368815023051 37.0 35.0 37.0 30.0 37.0 7 34.40032193320751 37.0 35.0 37.0 30.0 37.0 8 34.367627432673835 37.0 35.0 37.0 30.0 37.0 9 35.76602066525071 39.0 35.0 39.0 30.0 39.0 10-11 35.71397178900144 39.0 35.0 39.0 30.0 39.0 12-13 35.78489017757754 39.0 35.0 39.0 30.0 39.0 14-15 36.860397166277146 40.0 36.0 41.0 30.5 41.0 16-17 36.81699462307026 40.0 36.0 41.0 30.0 41.0 18-19 36.80302853779396 40.0 36.5 41.0 30.0 41.0 20-21 36.67531109846733 39.5 36.0 41.0 30.0 41.0 22-23 36.36426373090012 39.5 36.0 40.5 28.5 41.0 24-25 36.27433590172389 39.0 36.0 40.5 29.0 41.0 26-27 36.191964105803166 39.0 36.0 41.0 29.0 41.0 28-29 35.9534125808281 39.0 35.0 41.0 27.0 41.0 30-31 35.714612102276206 39.0 35.0 41.0 26.5 41.0 32-33 35.294098701561 38.0 34.0 40.0 25.0 41.0 34-35 35.103630748314714 38.0 34.0 40.0 25.0 41.0 36-37 34.76316496902924 38.0 34.0 40.0 24.0 41.0 38-39 34.00003843001693 36.5 33.0 39.5 24.0 41.0 40-41 34.15471924815552 37.0 33.0 40.0 24.0 41.0 42-43 34.068975475384455 37.0 33.0 40.0 23.5 41.0 44-45 33.923363767226306 37.0 33.0 40.0 23.0 41.0 46-47 33.70924966560743 37.0 33.0 40.0 22.5 41.0 48-49 33.333115080135 36.5 33.0 40.0 20.0 41.0 50-51 32.07004347454105 35.5 31.5 39.0 14.0 40.5 52-53 31.606021310426176 35.5 31.0 39.0 5.0 40.0 54-55 31.10090937014511 36.0 29.5 40.0 2.0 41.0 56-57 30.55495632676361 36.0 28.5 40.0 2.0 41.0 58-59 30.211033622524667 36.0 28.0 40.0 2.0 41.0 60-61 29.912098629563154 35.5 28.0 39.0 2.0 41.0 62-63 29.581129459132576 35.0 27.5 39.0 2.0 41.0 64-65 29.24490767211809 35.0 26.5 39.0 2.0 41.0 66-67 28.923329311116234 35.0 26.0 38.5 2.0 40.0 68-69 28.59681372147962 35.0 26.0 38.0 2.0 40.0 70-71 28.226287066616237 35.0 26.0 37.0 2.0 40.0 72-73 27.83277229560444 34.0 25.5 37.0 2.0 39.0 74-75 27.47182537437709 34.0 25.0 36.0 2.0 39.0 76-77 27.081688938466623 34.0 24.0 36.0 2.0 38.5 78-79 26.73241478424257 34.0 22.5 35.0 2.0 37.5 80-81 26.38025557363813 34.0 21.5 35.0 2.0 37.0 82-83 26.009018945250318 33.0 20.0 35.0 2.0 36.5 84-85 25.66457004899126 33.0 19.0 35.0 2.0 36.0 86-87 25.33575726067847 33.0 17.5 35.0 2.0 36.0 88-89 24.988197823888605 33.0 13.0 35.0 2.0 35.0 90-91 24.63157685091725 32.0 7.0 35.0 2.0 35.0 92-93 24.290807979417966 32.0 2.0 35.0 2.0 35.0 94-95 23.862424700308985 32.0 2.0 35.0 2.0 35.0 96-97 23.386661698503893 32.0 2.0 35.0 2.0 35.0 98-99 22.87419966684635 31.0 2.0 35.0 2.0 35.0 100-101 21.574245062888245 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.48691165313593743 1101 2 0.5520935511313105 1101 3 0.5253760221962871 1101 4 0.5825548242485752 1101 5 0.5590148290150196 1101 6 0.47616154730256 1101 7 0.45650396623724987 1101 8 0.5114507220317819 1101 9 0.6638427713271682 1101 10-11 0.6854697941859769 1101 12-13 0.6827173697984108 1101 14-15 0.7760629053897361 1101 16-17 0.7481095291112112 1101 18-19 0.7724104968431433 1101 20-21 0.7405655006496588 1101 22-23 0.7750599415842458 1101 24-25 0.752583058620047 1101 26-27 0.9752835324860953 1101 28-29 1.0435209650825925 1101 30-31 1.0302265748744546 1101 32-33 0.9674758196409456 1101 34-35 1.081839718714889 1101 36-37 1.0322341054236475 1101 38-39 0.9628824695619116 1101 40-41 1.0434040626472267 1101 42-43 1.2262701943970882 1101 44-45 1.2592807039478728 1101 46-47 1.2426724811900556 1101 48-49 1.1168952517688808 1101 50-51 1.036819342538486 1101 52-53 1.1117108996180463 1101 54-55 0.9794423471508757 1101 56-57 0.9373734305208465 1101 58-59 0.8216319911150158 1101 60-61 0.9251594330034294 1101 62-63 0.9852958291682192 1101 64-65 0.8263732127529373 1101 66-67 0.8572416363815556 1101 68-69 0.8776477148397106 1101 70-71 0.9728558414725477 1101 72-73 1.026112006496927 1101 74-75 1.0539734287791624 1101 76-77 1.0513034590068209 1101 78-79 0.9208887044532617 1101 80-81 1.0034453169126039 1101 82-83 1.0441474864480078 1101 84-85 0.9981150938362013 1101 86-87 0.9873908205128963 1101 88-89 1.0606519508981656 1101 90-91 1.0280501528323391 1101 92-93 1.1261796736855239 1101 94-95 1.2631568178113923 1101 96-97 1.3847724141793556 1101 98-99 1.4949423442803713 1101 100-101 1.8033822042454766 1102 1 0.5252214308620857 1102 2 0.49147167974689765 1102 3 0.49767857563444906 1102 4 0.6675041310834402 1102 5 0.5786662566303349 1102 6 0.4592616170125652 1102 7 0.4041651306081846 1102 8 0.6004272178649117 1102 9 0.6062099452539798 1102 10-11 0.6936245506741301 1102 12-13 0.6516155673328257 1102 14-15 0.6257177730756212 1102 16-17 0.6319051891986973 1102 18-19 0.6884665964125674 1102 20-21 0.8344200193521871 1102 22-23 0.7043004296353388 1102 24-25 0.6828658419264997 1102 26-27 0.8807578715305695 1102 28-29 0.7870665973182227 1102 30-31 0.9501351061795873 1102 32-33 0.9916478997709604 1102 34-35 1.0362129625980003 1102 36-37 0.9781733753878328 1102 38-39 0.852762064296158 1102 40-41 0.8928335854419203 1102 42-43 1.0299518107633858 1102 44-45 1.0663287110343305 1102 46-47 1.1206059031740523 1102 48-49 0.9593766147916583 1102 50-51 1.101108432594387 1102 52-53 1.0734107949062661 1102 54-55 0.9635924980540942 1102 56-57 0.9711600815653156 1102 58-59 0.7471232076125958 1102 60-61 0.8707444078487008 1102 62-63 0.918743603207723 1102 64-65 0.8906469965216459 1102 66-67 0.9751127540885811 1102 68-69 0.9472982268734569 1102 70-71 0.9118572956397237 1102 72-73 0.9585023886690571 1102 74-75 1.0361049250225527 1102 76-77 0.9337012147556614 1102 78-79 0.8685764835920651 1102 80-81 0.8994436104363075 1102 82-83 0.9249935061773229 1102 84-85 0.9130352152856496 1102 86-87 0.9577919137312598 1102 88-89 1.11824865692925 1102 90-91 1.135926726287213 1102 92-93 1.2243399418349021 1102 94-95 1.2107742299641373 1102 96-97 1.2734148415580115 1102 98-99 1.4265933698784963 1102 100-101 1.6378752649060395 1103 1 0.3731425254766947 1103 2 0.4510569800356521 1103 3 0.42125295004252195 1103 4 0.5657437110885795 1103 5 0.4840929698529308 1103 6 0.38392863396549615 1103 7 0.2833726174731339 1103 8 0.32287942326558294 1103 9 0.4383546750391787 1103 10-11 0.5470637298384702 1103 12-13 0.4510171389359954 1103 14-15 0.6091542855972705 1103 16-17 0.5758157103979613 1103 18-19 0.5165723158572391 1103 20-21 0.5924615541507947 1103 22-23 0.47812475753379147 1103 24-25 0.527514252305096 1103 26-27 0.7032733381943999 1103 28-29 0.6898223369329557 1103 30-31 0.6436635538639521 1103 32-33 0.6580336156092699 1103 34-35 0.7267592375650906 1103 36-37 0.751192521555538 1103 38-39 0.6823272735159307 1103 40-41 0.5838159613579137 1103 42-43 0.7372469443719822 1103 44-45 0.7579934369625718 1103 46-47 0.6749487991044418 1103 48-49 0.7878687795198971 1103 50-51 0.8020281247020264 1103 52-53 0.8413073313487018 1103 54-55 0.7434453723762324 1103 56-57 0.6839520128874206 1103 58-59 0.5510856370956958 1103 60-61 0.6447018772129489 1103 62-63 0.6773352181826517 1103 64-65 0.7074078673125932 1103 66-67 0.7583169772443554 1103 68-69 0.7375999007024703 1103 70-71 0.7074633276454279 1103 72-73 0.7735144702764956 1103 74-75 0.6708955826754739 1103 76-77 0.7322464099912231 1103 78-79 0.7565824885772443 1103 80-81 0.837649872388873 1103 82-83 0.7859171669248042 1103 84-85 0.8612310912232992 1103 86-87 0.845836311289279 1103 88-89 0.8987866459646909 1103 90-91 0.9779208057411815 1103 92-93 0.9895628216837196 1103 94-95 1.0649391772046535 1103 96-97 1.1241702859081215 1103 98-99 1.1844944360725194 1103 100-101 1.398076167424314 1104 1 0.2897468120556148 1104 2 0.21758631320328803 1104 3 0.2826826745470328 1104 4 0.4060353433853834 1104 5 0.271210911797958 1104 6 0.18860796069703412 1104 7 0.2387411416554741 1104 8 0.24497654589976037 1104 9 0.33139053076609315 1104 10-11 0.46947798310816324 1104 12-13 0.35726333357688134 1104 14-15 0.37785024080982055 1104 16-17 0.4933329877708985 1104 18-19 0.4261169923023189 1104 20-21 0.48844369613912875 1104 22-23 0.3847595853341659 1104 24-25 0.4738066127286942 1104 26-27 0.42887928342336323 1104 28-29 0.3942439696106348 1104 30-31 0.5090245271017437 1104 32-33 0.4627243844822502 1104 34-35 0.5382511562334003 1104 36-37 0.6706426433409334 1104 38-39 0.675684489213559 1104 40-41 0.6244326877426545 1104 42-43 0.7304908507235908 1104 44-45 0.7945288288179526 1104 46-47 0.6702641935231384 1104 48-49 0.7271008000147674 1104 50-51 0.5599816119539724 1104 52-53 0.6257918605309101 1104 54-55 0.6198374300809029 1104 56-57 0.7136816502891712 1104 58-59 0.6667412650237381 1104 60-61 0.5928022089290614 1104 62-63 0.6753398286097934 1104 64-65 0.7329481084118079 1104 66-67 0.5884250949684748 1104 68-69 0.5988080108228218 1104 70-71 0.6697014893991167 1104 72-73 0.6634320079707514 1104 74-75 0.6835226590809711 1104 76-77 0.7271963509264907 1104 78-79 0.656444272373907 1104 80-81 0.5715589895705833 1104 82-83 0.5732137435472922 1104 84-85 0.706717238534555 1104 86-87 0.6991846693619657 1104 88-89 0.799543983670123 1104 90-91 0.7964977122921226 1104 92-93 0.9590555270014356 1104 94-95 0.9934781994073774 1104 96-97 0.9771339812530861 1104 98-99 1.0886394121922436 1104 100-101 1.379198111008531 1105 1 -0.024691754199341887 1105 2 0.028663769626550817 1105 3 0.13566836248265446 1105 4 0.19334770607836305 1105 5 0.21268525044808229 1105 6 0.21592676898724505 1105 7 0.22973872837246034 1105 8 0.3231114707002334 1105 9 0.2327761734995235 1105 10-11 0.11675552832910085 1105 12-13 0.1520652218396279 1105 14-15 0.016735990657451794 1105 16-17 0.15119093934028882 1105 18-19 0.09583084607857728 1105 20-21 0.3927486695587419 1105 22-23 0.408601232247193 1105 24-25 0.41102918694009816 1105 26-27 0.6236435679058374 1105 28-29 0.5284320921450032 1105 30-31 0.494908548661698 1105 32-33 0.5501830317048331 1105 34-35 0.5463967563032455 1105 36-37 0.5999235696217227 1105 38-39 0.6775553726807999 1105 40-41 0.6730105525388268 1105 42-43 0.7179051995281469 1105 44-45 0.6307924497279274 1105 46-47 0.6239577188979553 1105 48-49 0.7843235174914156 1105 50-51 0.7137138925867603 1105 52-53 0.48846475624559105 1105 54-55 0.63175967614794 1105 56-57 0.6956627649838474 1105 58-59 0.7430573571655152 1105 60-61 0.7276622387817859 1105 62-63 0.6850020988632153 1105 64-65 0.6879409625554658 1105 66-67 0.8405098386388303 1105 68-69 0.7844552698238978 1105 70-71 0.6392717164169603 1105 72-73 0.7797730630777941 1105 74-75 0.6749390958091475 1105 76-77 0.6146027197228356 1105 78-79 0.6437496324757959 1105 80-81 0.6123871082274128 1105 82-83 0.7057376126087469 1105 84-85 0.7121164304114416 1105 86-87 0.8445433305629528 1105 88-89 0.8589063743199077 1105 90-91 0.912341489706666 1105 92-93 1.0146312296973932 1105 94-95 1.05783736725855 1105 96-97 1.1144623909927631 1105 98-99 1.229182854767778 1105 100-101 1.4199292913106554 1106 1 0.07606237013279227 1106 2 0.21515449751641214 1106 3 0.2688331180589145 1106 4 0.340888727955452 1106 5 0.2767728121656532 1106 6 0.2467643457790345 1106 7 0.23200246205028208 1106 8 0.19878420760687732 1106 9 0.11941640292641864 1106 10-11 0.20421429434578897 1106 12-13 0.3204210677806074 1106 14-15 0.2859938683359218 1106 16-17 0.29024227624980625 1106 18-19 0.14992647050062402 1106 20-21 0.2700623949390817 1106 22-23 0.3388346598143812 1106 24-25 0.3982536222706443 1106 26-27 0.2896526385817637 1106 28-29 0.2869474899895792 1106 30-31 0.42117082080758905 1106 32-33 0.4705121658685556 1106 34-35 0.4604984384256525 1106 36-37 0.47106256936612567 1106 38-39 0.6315185507737269 1106 40-41 0.41572060173145786 1106 42-43 0.3180580894287388 1106 44-45 0.37964951067738895 1106 46-47 0.4865656462813206 1106 48-49 0.44961332796182774 1106 50-51 0.4861308856835649 1106 52-53 0.5336286409857394 1106 54-55 0.30454173873821944 1106 56-57 0.2404424239704035 1106 58-59 0.4729943762913642 1106 60-61 0.41525945413390275 1106 62-63 0.3755347175986792 1106 64-65 0.38212914533073317 1106 66-67 0.5279670171938484 1106 68-69 0.5683087569835976 1106 70-71 0.4673431145731577 1106 72-73 0.5269433126054217 1106 74-75 0.6075441110674511 1106 76-77 0.5506854432841273 1106 78-79 0.5510993999059899 1106 80-81 0.5370375731802604 1106 82-83 0.5022884919557917 1106 84-85 0.48778905392796545 1106 86-87 0.5962799002370467 1106 88-89 0.5385595446275993 1106 90-91 0.5361866113435809 1106 92-93 0.6025546284021317 1106 94-95 0.6414088531920363 1106 96-97 0.7041760529279983 1106 98-99 0.8092331439526035 1106 100-101 0.9891934454657196 1107 1 0.008423199262676206 1107 2 0.0067868172919389735 1107 3 0.1984952477802331 1107 4 0.3904776030697974 1107 5 0.11450763087230342 1107 6 0.210293689492012 1107 7 0.2344803425873252 1107 8 0.13766847353272738 1107 9 0.13219971719856716 1107 10-11 0.15541255920884112 1107 12-13 0.1709428648073512 1107 14-15 0.30497133088682915 1107 16-17 0.20187915262071243 1107 18-19 0.24597821037114187 1107 20-21 0.17366299344524094 1107 22-23 0.20638485735251777 1107 24-25 0.4042490091651558 1107 26-27 0.1471444185891002 1107 28-29 0.22564034702693192 1107 30-31 0.3057331197404096 1107 32-33 0.507368027053694 1107 34-35 0.33156126234619876 1107 36-37 0.35645574098619903 1107 38-39 0.44971825997926373 1107 40-41 0.2365995677115933 1107 42-43 0.29255159031048805 1107 44-45 0.42154290911869197 1107 46-47 0.28399357083898735 1107 48-49 0.44618517801438173 1107 50-51 0.22083749930555996 1107 52-53 0.34723541119636536 1107 54-55 0.26631413282990835 1107 56-57 0.11510722661672546 1107 58-59 0.4625001403877711 1107 60-61 0.375868165305814 1107 62-63 0.24069702947319982 1107 64-65 0.361058470343643 1107 66-67 0.4835115179792311 1107 68-69 0.3890747840742286 1107 70-71 0.25156504988537876 1107 72-73 0.3255811131134756 1107 74-75 0.40782190039450583 1107 76-77 0.3598513548559872 1107 78-79 0.2339973068911938 1107 80-81 0.23807651499085125 1107 82-83 0.25449881972679833 1107 84-85 0.2877117884694904 1107 86-87 0.47143161866170047 1107 88-89 0.4486637428311049 1107 90-91 0.47433551382743033 1107 92-93 0.5415435982595653 1107 94-95 0.42780503292633654 1107 96-97 0.5067143361696864 1107 98-99 0.5100344065778337 1107 100-101 0.5791630609516645 1108 1 0.17183765883082813 1108 2 0.1485577111527654 1108 3 0.13324166536331816 1108 4 0.3966556915679291 1108 5 0.17905939430288242 1108 6 0.2702176463629655 1108 7 0.2408295118086201 1108 8 0.17396662989102651 1108 9 0.272888273505707 1108 10-11 0.2976041283339441 1108 12-13 0.12272496569299562 1108 14-15 0.32354981285891427 1108 16-17 0.2570556291828865 1108 18-19 0.3286571291647107 1108 20-21 0.3016670223716815 1108 22-23 0.2759543575749319 1108 24-25 0.45162185320100434 1108 26-27 0.3732064755860236 1108 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34-35 0.04394219963101165 2312 36-37 0.132569647715421 2312 38-39 0.12941395826205593 2312 40-41 0.12017944443397255 2312 42-43 0.1921786510044754 2312 44-45 0.298398950449382 2312 46-47 0.19958941881937875 2312 48-49 0.2511562165949357 2312 50-51 0.09661387202936567 2312 52-53 0.2453682960370216 2312 54-55 0.15175287593698883 2312 56-57 0.07817572267319761 2312 58-59 -0.04473742963890004 2312 60-61 0.01287925494710862 2312 62-63 0.06355974606750436 2312 64-65 0.14204385806876374 2312 66-67 0.0830574002195803 2312 68-69 0.048183335083301415 2312 70-71 0.11317190551729794 2312 72-73 0.18843283501893993 2312 74-75 0.1447673966868912 2312 76-77 0.16550020337776417 2312 78-79 0.136311903710439 2312 80-81 0.0804072054628584 2312 82-83 0.03971537224034094 2312 84-85 0.0914579066154495 2312 86-87 0.06520818901288905 2312 88-89 0.07177755865296476 2312 90-91 0.13377109308853008 2312 92-93 0.08716760377351918 2312 94-95 0.009321355364413364 2312 96-97 0.19969072251811326 2312 98-99 0.14052488850929024 2312 100-101 0.023848488142501623 2313 1 -0.560602302584833 2313 2 -0.33739795552240537 2313 3 -0.09359324885830134 2313 4 0.08821743102188151 2313 5 -0.2541657572098899 2313 6 -0.2035242953390224 2313 7 -0.16396147636161373 2313 8 -0.09427900227721864 2313 9 0.01589879641299774 2313 10-11 -0.11641356168883021 2313 12-13 -0.1783745261018197 2313 14-15 -0.43724164961783174 2313 16-17 -0.23489578489532192 2313 18-19 -0.13031432292115852 2313 20-21 -0.12608982154487336 2313 22-23 -0.17664145783087548 2313 24-25 -0.4437656745677572 2313 26-27 -0.33023524710151975 2313 28-29 -0.3518833364626559 2313 30-31 -0.4705441155701493 2313 32-33 -0.8316335314783032 2313 34-35 -0.43607816490481355 2313 36-37 -0.13290151604286393 2313 38-39 -0.20914105833296048 2313 40-41 -0.09165060162245453 2313 42-43 -0.18038151798987911 2313 44-45 -0.15252203578304346 2313 46-47 -0.20795527064068153 2313 48-49 -0.3838228183691115 2313 50-51 -0.37381784376870186 2313 52-53 -0.3390491093752388 2313 54-55 -0.2545522620808889 2313 56-57 -0.23144034542089642 2313 58-59 -0.3388645294384638 2313 60-61 -0.382860285904993 2313 62-63 -0.3235307179177447 2313 64-65 -0.3077212858324252 2313 66-67 -0.4126577686124051 2313 68-69 -0.4194677901537567 2313 70-71 -0.2882524403366311 2313 72-73 -0.39983669760223606 2313 74-75 -0.4375444157384152 2313 76-77 -0.4988735725094209 2313 78-79 -0.34710401376876376 2313 80-81 -0.5249536370165231 2313 82-83 -0.3393917414948575 2313 84-85 -0.4212547582873505 2313 86-87 -0.48319617548996874 2313 88-89 -0.5247668183810212 2313 90-91 -0.40344105832476984 2313 92-93 -0.32993387523541173 2313 94-95 -0.2618988505320594 2313 96-97 -0.2695273278919146 2313 98-99 -0.2592693436687661 2313 100-101 -0.3527811716195899 2314 1 -0.6672296166296938 2314 2 -0.869887829917463 2314 3 -0.5343165984679139 2314 4 -0.7924813971365268 2314 5 -0.6753553411586424 2314 6 -0.4884498017983816 2314 7 -0.3829324176643425 2314 8 -0.5098881999270546 2314 9 -0.4523977088739315 2314 10-11 -0.6033839742242861 2314 12-13 -0.5693603219031189 2314 14-15 -0.7015863294301141 2314 16-17 -0.7613700032533117 2314 18-19 -2.4309329552923913 2314 20-21 -1.1517785120848814 2314 22-23 -0.8464477347841779 2314 24-25 -0.9508943892080879 2314 26-27 -1.0108883956913175 2314 28-29 -0.6652704818262052 2314 30-31 -0.7222388225415983 2314 32-33 -0.796169504995909 2314 34-35 -0.818694779188391 2314 36-37 -0.8165348780599899 2314 38-39 -0.6453182937485877 2314 40-41 -0.622843240049697 2314 42-43 -0.9002113675489554 2314 44-45 -0.9066208121656061 2314 46-47 -0.7449815392430921 2314 48-49 -0.7308131370003181 2314 50-51 -0.7476176984538121 2314 52-53 -0.8114243613194816 2314 54-55 -0.8621671595308804 2314 56-57 -0.7924557594412143 2314 58-59 -0.785883044767953 2314 60-61 -0.7962390415003178 2314 62-63 -0.8037518229312575 2314 64-65 -0.8635216103096077 2314 66-67 -0.8395818061087503 2314 68-69 -0.884347643692557 2314 70-71 -1.0017933820998017 2314 72-73 -0.9902262060802052 2314 74-75 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-1.582446997714186 2315 32-33 -1.9103825228664348 2315 34-35 -1.9998619195738172 2315 36-37 -1.6321199856979192 2315 38-39 -1.7805521548852212 2315 40-41 -2.696539022425778 2315 42-43 -2.2701318401635078 2315 44-45 -1.9353926697862605 2315 46-47 -1.5322774153209977 2315 48-49 -1.9891005056617743 2315 50-51 -1.7187704805679687 2315 52-53 -1.5667626645380466 2315 54-55 -1.4734248993025716 2315 56-57 -1.6497344352945866 2315 58-59 -1.8270697051686717 2315 60-61 -1.5092955053511616 2315 62-63 -1.3859287040426373 2315 64-65 -1.3736427154833137 2315 66-67 -1.4447091814241872 2315 68-69 -1.7111362808136903 2315 70-71 -1.3290712321938933 2315 72-73 -1.293476961090061 2315 74-75 -1.3473666698884195 2315 76-77 -1.3217931791899815 2315 78-79 -1.4541279771742062 2315 80-81 -1.5818245248895124 2315 82-83 -1.4277175813373297 2315 84-85 -1.5121560916693078 2315 86-87 -1.61026298927354 2315 88-89 -1.6170017503387761 2315 90-91 -1.6039710626304498 2315 92-93 -1.6283829085611004 2315 94-95 -1.633317622147132 2315 96-97 -1.7868328720823001 2315 98-99 -1.744790661855241 2315 100-101 -1.9632671344231234 2316 1 -0.8384845025677237 2316 2 -0.9770256349339199 2316 3 -1.2826045567019406 2316 4 -1.2386591749143037 2316 5 -0.7324867904833496 2316 6 -0.8196421295704823 2316 7 -0.8803208639247018 2316 8 -0.9705232243283675 2316 9 -1.24966630494702 2316 10-11 -1.3131926225048431 2316 12-13 -1.1458422876277865 2316 14-15 -1.193589575372684 2316 16-17 -1.890255736995222 2316 18-19 -1.2811557222881902 2316 20-21 -1.719082858924061 2316 22-23 -1.2174907264990864 2316 24-25 -1.1493517493261152 2316 26-27 -1.2670561178567965 2316 28-29 -1.5834280802826797 2316 30-31 -1.4638017325404675 2316 32-33 -1.4870455236090052 2316 34-35 -1.844781988358747 2316 36-37 -1.5861659074550616 2316 38-39 -1.5612842453999392 2316 40-41 -1.9177567345452147 2316 42-43 -2.147911446995103 2316 44-45 -2.376892064009887 2316 46-47 -2.1144009468018297 2316 48-49 -2.793605215084529 2316 50-51 -2.198456899452559 2316 52-53 -2.8140489896250074 2316 54-55 -3.972420365750377 2316 56-57 -2.6707622853747033 2316 58-59 -2.1070853146578656 2316 60-61 -1.8735740999499804 2316 62-63 -2.5097004553225872 2316 64-65 -2.5626793903572747 2316 66-67 -2.313958221952582 2316 68-69 -2.3353225160112494 2316 70-71 -2.503549232741044 2316 72-73 -5.518717614546894 2316 74-75 -3.248857802103995 2316 76-77 -2.5999905684892326 2316 78-79 -2.5536142866282248 2316 80-81 -2.4862508742075207 2316 82-83 -2.5188807501339845 2316 84-85 -2.572807781980792 2316 86-87 -2.419309356569432 2316 88-89 -2.2901043258654106 2316 90-91 -2.301626192188426 2316 92-93 -2.5258887122895572 2316 94-95 -2.820081213033127 2316 96-97 -2.92737538195059 2316 98-99 -3.275417628674326 2316 100-101 -3.375658872415073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 134639.0 3 21623.0 4 18036.0 5 17095.0 6 19095.0 7 23012.0 8 28253.0 9 33286.0 10 39010.0 11 46069.0 12 48318.0 13 54783.0 14 62735.0 15 75910.0 16 97089.0 17 130744.0 18 189878.0 19 283361.0 20 373820.0 21 267861.0 22 153108.0 23 133356.0 24 139423.0 25 150101.0 26 164730.0 27 187109.0 28 216442.0 29 252930.0 30 298212.0 31 355096.0 32 428962.0 33 531285.0 34 682134.0 35 946054.0 36 1373561.0 37 1485654.0 38 1071274.0 39 160570.0 40 147.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.098536322200427 49.912899274834515 23.354759400392094 16.633805002572963 2 15.913105298329375 51.64142474011067 17.207051737176272 15.238418224383683 3 46.71951784452545 19.718567945832376 18.114830117871666 15.447084091770508 4 15.998994545229866 18.071597336163197 19.067186807444074 46.86222131116286 5 15.876526375834304 49.101682157497436 20.036003455493308 14.985788011174957 6 46.95237939255824 19.227129684440065 17.951974618564854 15.868516304436838 7 16.793398080024467 18.616325641657042 49.8024738293852 14.78780244893329 8 46.56972472505213 19.40185508102468 18.812012947178957 15.216407246744232 9 46.20052015660657 19.06564017788222 19.33764017937945 15.396199486131762 10-11 15.582370174714866 34.293298695509186 34.37473257940669 15.749598550369253 12-13 15.404866208438051 19.37477370952263 34.34199957341949 30.878360508619824 14-15 15.56328913803208 49.7852821215691 18.870609786235654 15.78081895416317 16-17 30.86773138319161 19.181369757860804 18.962514012105675 30.988384846841914 18-19 15.738523866806474 34.35387742777063 18.968576542912164 30.93902216251073 20-21 31.080144272207562 34.41943047598359 18.99479285217712 15.505632399631732 22-23 31.02468763470473 19.135545509243737 34.25948134189856 15.580285514152978 24-25 15.67089041227241 34.187086363908925 19.51254284340747 30.629480380411195 26-27 15.899760287187641 25.093572188636443 43.119086986517615 15.887580537658296 28-29 28.026290569133206 19.027323030052496 37.213995369314084 15.732391031500207 30-31 25.69472044358993 20.28049879547711 37.66654615981454 16.358234601118422 32-33 19.541373871945133 22.978768741438433 39.18698903329066 18.292868353325776 34-35 18.659432424483054 21.8331191618949 39.94822464725328 19.55922376636877 36-37 18.580769260092893 21.872892103235507 40.20386046829038 19.34247816838122 38-39 18.845864016289195 22.309848310856797 39.768796726070335 19.075490946783674 40-41 19.013851094975728 21.893606953780665 39.765656222075535 19.32688572916807 42-43 19.534613585802198 22.23103077198179 39.74324259789094 18.49111304432507 44-45 18.706850663231464 22.451542800156794 39.64314882223675 19.19845771437499 46-47 18.985951856988173 22.089672381495834 39.53953802727309 19.384837734242904 48-49 19.19495880635526 22.264333401555998 39.60642941891125 18.93427837317749 50-51 19.323792564063382 22.135504255675517 38.81657922294522 19.724123957315882 52-53 19.560179187296374 23.112747872174243 37.79221532408711 19.534857616442277 54-55 20.207409445982368 24.117396910097565 35.98753090589986 19.687662738020215 56-57 20.559760386529106 24.61174548655093 34.9010267986141 19.92746732830587 58-59 20.559152942299068 25.670298510988264 33.73939640145947 20.03115214525319 60-61 20.73025519978263 25.941841352330542 33.15945101996514 20.168452427921686 62-63 20.59816753516486 25.793724986950423 33.58661912328842 20.0214883545963 64-65 20.40249014360255 25.713297829347365 33.400558154990165 20.48365387205992 66-67 20.550416517462754 25.123577324297656 34.208458730103494 20.1175474281361 68-69 20.211727258112973 24.83503665776694 34.56019964029088 20.393036443829207 70-71 20.094511160332555 24.594044704197955 35.26424749380397 20.047196641665515 72-73 19.82506868646797 24.37911141882158 34.98867140402142 20.807148490689027 74-75 20.325877600780647 24.575081057552335 34.410990205738706 20.68805113592832 76-77 20.801606920189222 25.12931644758718 33.79581619447124 20.273260437752352 78-79 20.50834109882026 25.00687913862099 33.69805045936462 20.786729303194125 80-81 20.346843168234958 25.46005655758331 33.54294063885934 20.65015963532239 82-83 21.34719958570933 25.690633343308317 32.718878878257115 20.243288192725238 84-85 20.879466277941404 26.303918055626557 32.23566715514575 20.58094851128629 86-87 20.83951501269889 26.102245779673677 32.34187288349885 20.716366324128582 88-89 20.731372102489583 25.876239028990504 32.14755015924985 21.244838709270056 90-91 20.75454030314915 25.820208275013385 32.2067287015541 21.21852272028337 92-93 20.77521637191513 26.179291072498543 32.28391467561133 20.761577879975004 94-95 20.973020832773038 25.880538437414568 31.73559981131912 21.410840918493275 96-97 20.7527633045742 25.772301486372147 32.56443447162216 20.9105007374315 98-99 20.851207301362187 26.19885720721138 31.709188634331092 21.24074685709534 100-101 21.359794090194033 25.944195973460683 31.51693873771752 21.179071198627767 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16274.0 1 9904.5 2 2625.0 3 1538.5 4 1320.0 5 1194.5 6 1310.0 7 1561.0 8 1490.5 9 1544.0 10 2063.0 11 13588.0 12 247355.0 13 277239.5 14 73958.0 15 64361.5 16 68183.5 17 73325.5 18 77035.0 19 78660.0 20 79409.5 21 80374.5 22 81830.0 23 84071.5 24 88048.5 25 93952.0 26 101025.5 27 108874.0 28 117598.5 29 126325.5 30 135127.0 31 144941.5 32 156573.0 33 170805.5 34 194947.5 35 210944.0 36 217894.0 37 232246.5 38 243551.5 39 253713.0 40 265760.0 41 274416.5 42 281333.0 43 294848.0 44 314000.5 45 322933.0 46 322432.5 47 329467.5 48 340690.0 49 348227.0 50 349722.5 51 344750.5 52 340607.5 53 342076.5 54 325212.0 55 297749.5 56 295170.5 57 282366.0 58 262077.0 59 221984.5 60 161203.0 61 123731.0 62 92431.0 63 66218.0 64 45776.5 65 29649.0 66 18782.5 67 11793.5 68 7908.0 69 5721.5 70 4548.0 71 3887.5 72 3564.0 73 3264.5 74 3013.0 75 2840.0 76 2689.0 77 2552.5 78 2412.5 79 2276.0 80 2146.5 81 2010.0 82 1886.0 83 1755.5 84 1597.5 85 1371.0 86 1125.5 87 942.0 88 820.0 89 674.0 90 502.0 91 383.5 92 287.0 93 195.5 94 122.5 95 87.0 96 60.5 97 37.5 98 16.5 99 9.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09895495599950069 2 0.0820401383293602 3 0.08803372491120655 4 0.19624554630232643 5 0.07492450745762061 6 0.0915401133171229 7 0.09685112295595089 8 0.08596729334398652 9 0.0782438884818881 10-11 0.07650004464801237 12-13 0.06966492484874609 14-15 0.07652342057071847 16-17 0.06668215711144658 18-19 0.05921121221457414 20-21 0.05426954215450269 22-23 0.04410569096188649 24-25 0.04641523212525006 26-27 0.045723304813149235 28-29 0.04461996126142089 30-31 0.03562490620411014 32-33 0.03911726905640283 34-35 0.05151118327518183 36-37 0.04723338941996388 38-39 0.05114651888096653 40-41 0.048743474026778526 42-43 0.046980929454737905 44-45 0.050819255963081 46-47 0.044320749450782695 48-49 0.04002893004194108 50-51 0.04438152684981858 52-53 0.03997282782744642 54-55 0.04351661770969255 56-57 0.04252547858695352 58-59 0.049486828368832794 60-61 0.048243229280867786 62-63 0.05347943596703621 64-65 0.049192291742735816 66-67 0.04391868358023762 68-69 0.04430672389715903 70-71 0.05289503789938348 72-73 0.04058527700234647 74-75 0.04804219634559525 76-77 0.04674717022767681 78-79 0.04522306006723851 80-81 0.04464801236866822 82-83 0.04381582952033074 84-85 0.04470878976770411 86-87 0.049070736944664045 88-89 0.041969131626548126 90-91 0.04499865120925985 92-93 0.04145486132701373 94-95 0.04448438090972546 96-97 0.030514929500554712 98-99 0.03682175344666292 100-101 0.025292748368009956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0694765E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.970056998009156 #Duplication Level Percentage of deduplicated Percentage of total 1 90.94935362462382 37.26200201932929 2 4.943606141421591 4.050796507795014 3 1.4262820769592697 1.7530457396478047 4 0.6480084962434528 1.0619578010515387 5 0.3851842629043675 0.7890510602964041 6 0.25011918343920153 0.6148438321079757 7 0.17938582388509047 0.5144613200444893 8 0.12656181774452574 0.41481959094118925 9 0.1103853855337654 0.40702459833590243 >10 0.9166613218486594 7.468856260017284 >50 0.03986017318119708 1.0713882856484054 >100 0.01532388996865855 1.281384516318517 >500 0.003971915248489372 1.1965597461078412 >1k 0.004302908185822205 3.865347691005249 >5k 6.068203851800546E-4 1.81757655966128 >10k+ 3.8615842693276204E-4 36.43088447169177 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1490648 13.938108972006397 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 36553 0.34178404107056115 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC 35371 0.3307319048151128 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14265 0.13338301496105806 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 12833 0.11999328643499879 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 11688 0.10928711383560087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.740147632977443E-5 0.0 0.0 0.0 0.0 2 8.415332174199246E-5 0.0 0.0 9.350369082443607E-6 0.0 3 1.3090516715421048E-4 0.0 0.0 9.350369082443607E-6 0.0 4 1.496059053190977E-4 0.0 0.0 9.350369082443607E-6 0.0 5 1.589562744015413E-4 9.350369082443607E-6 0.0 9.350369082443607E-6 9.350369082443607E-6 6 1.7765701256642854E-4 1.8700738164887214E-5 0.0 9.350369082443607E-6 1.8700738164887214E-5 7 2.0570811981375934E-4 1.8700738164887214E-5 0.0 9.350369082443607E-6 1.8700738164887214E-5 8 2.1505848889620297E-4 1.8700738164887214E-5 0.0 9.350369082443607E-6 1.8700738164887214E-5 9 2.3375922706109017E-4 1.8700738164887214E-5 0.0 9.350369082443607E-6 1.8700738164887214E-5 10-11 2.805110724733082E-4 1.8700738164887214E-5 0.0 9.350369082443607E-6 1.8700738164887214E-5 12-13 3.7868994783896604E-4 2.805110724733082E-5 0.0 9.350369082443607E-6 1.8700738164887214E-5 14-15 4.2544179325118407E-4 2.805110724733082E-5 0.0 9.350369082443607E-6 1.8700738164887214E-5 16-17 4.5349290049851495E-4 2.805110724733082E-5 0.0 9.350369082443607E-6 2.805110724733082E-5 18-19 5.142702995343984E-4 3.272629178855263E-5 0.0 9.350369082443607E-6 2.805110724733082E-5 20-21 5.46996591322951E-4 3.740147632977443E-5 0.0 9.350369082443607E-6 2.805110724733082E-5 22-23 5.890732521939472E-4 4.207666087099623E-5 0.0 9.350369082443607E-6 2.805110724733082E-5 24-25 6.919273121008269E-4 4.6751845412218035E-5 0.0 9.350369082443607E-6 3.740147632977443E-5 26-27 7.947813720077066E-4 6.545258357710524E-5 0.0 1.402555362366541E-5 3.740147632977443E-5 28-29 8.742595092084773E-4 7.480295265954886E-5 0.0 1.8700738164887214E-5 3.740147632977443E-5 30-31 9.537376464092478E-4 8.415332174199246E-5 0.0 1.8700738164887214E-5 4.6751845412218035E-5 32-33 0.0010378909681512402 8.882850628321427E-5 0.0 1.8700738164887214E-5 5.610221449466164E-5 34-35 0.0010893179981046803 9.350369082443607E-5 0.0 2.3375922706109017E-5 5.610221449466164E-5 36-37 0.0011734713198466727 9.350369082443607E-5 0.0 4.207666087099623E-5 5.610221449466164E-5 38-39 0.0012435990879649996 9.350369082443607E-5 0.0 4.6751845412218035E-5 5.610221449466164E-5 40-41 0.0013184020406245486 9.817887536565787E-5 0.0 4.6751845412218035E-5 5.610221449466164E-5 42-43 0.001379179439660432 1.0285405990687967E-4 0.0 4.6751845412218035E-5 5.610221449466164E-5 44-45 0.0014960590531909771 1.1220442898932328E-4 0.0 4.6751845412218035E-5 6.545258357710524E-5 46-47 0.0015381357140619733 1.1220442898932328E-4 0.0 4.6751845412218035E-5 7.012776811832704E-5 48-49 0.0016082634821803004 1.1220442898932328E-4 0.0 4.6751845412218035E-5 7.480295265954886E-5 50-51 0.0016737160657574056 1.1220442898932328E-4 0.0 4.6751845412218035E-5 7.480295265954886E-5 52-53 0.001804621232911616 1.2155479807176688E-4 0.0 4.6751845412218035E-5 7.480295265954886E-5 54-55 0.0019495519536894918 1.2155479807176688E-4 0.0 5.610221449466164E-5 7.947813720077066E-5 56-57 0.002066431567220037 1.2155479807176688E-4 0.0 5.610221449466164E-5 8.415332174199246E-5 58-59 0.002220712657080357 1.262299826129887E-4 0.0 7.012776811832704E-5 8.415332174199246E-5 60-61 0.002374993746940676 1.3090516715421048E-4 0.0 8.882850628321427E-5 8.415332174199246E-5 62-63 0.0025479755749658827 1.4025553623665408E-4 0.0 9.350369082443607E-5 9.350369082443607E-5 64-65 0.0027443333256971982 1.496059053190977E-4 0.0 9.350369082443607E-5 9.350369082443607E-5 66-67 0.003038869951794172 1.589562744015413E-4 0.0 9.350369082443607E-5 9.350369082443607E-5 68-69 0.0033193810242674805 1.683066434839849E-4 0.0 1.0285405990687967E-4 9.350369082443607E-5 70-71 0.003660669495776672 1.8233219710765035E-4 0.0 1.1220442898932328E-4 1.1220442898932328E-4 72-73 0.0040066331518270855 1.8700738164887214E-4 0.0 1.1220442898932328E-4 1.262299826129887E-4 74-75 0.0044133742069133826 1.9168256619009393E-4 0.0 1.1220442898932328E-4 1.3090516715421048E-4 76-77 0.004787388970211127 1.9635775073131574E-4 0.0 1.1220442898932328E-4 1.3090516715421048E-4 78-79 0.0052595826088745285 1.9635775073131574E-4 0.0 1.2155479807176688E-4 1.3090516715421048E-4 80-81 0.005773852908408927 1.9635775073131574E-4 0.0 1.2155479807176688E-4 1.355803516954323E-4 82-83 0.0065125320659219724 2.0570811981375934E-4 0.0 1.2155479807176688E-4 1.402555362366541E-4 84-85 0.007494320819578551 2.0570811981375934E-4 0.0 1.2155479807176688E-4 1.402555362366541E-4 86-87 0.00876129583024966 2.1505848889620297E-4 0.0 1.309051671542105E-4 1.402555362366541E-4 88-89 0.010911880719211689 2.1505848889620297E-4 0.0 1.589562744015413E-4 1.449307207778759E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 328715 0.0 91.87611 5 GGTATCA 330095 0.0 91.61874 8 TGGTATC 330155 0.0 91.57181 7 GTATCAA 330230 0.0 91.54189 9 GTGGTAT 330880 0.0 91.44235 6 AAGCAGT 332465 0.0 91.28245 1 AGCAGTG 334200 0.0 90.77126 2 GCAGTGG 333590 0.0 90.73568 3 CAGTGGT 333595 0.0 90.45487 4 TCAACGC 329170 0.0 46.45661 12-13 AACGCAG 330160 0.0 46.33467 14-15 CAACGCA 329290 0.0 46.28097 12-13 ATCAACG 329500 0.0 46.26687 10-11 CGCAGAG 331565 0.0 46.19546 16-17 ACGCAGA 330765 0.0 46.119934 14-15 TATCAAC 331865 0.0 46.013744 10-11 CAGAGTA 329395 0.0 45.865917 18-19 AGAGTAC 323640 0.0 45.7525 18-19 GAGTACT 190110 0.0 45.613945 20-21 GCAGAGT 333585 0.0 45.54194 16-17 >>END_MODULE