FastQCFastQC Report
Sat 18 Jun 2016
SRR578556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10694765
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1799471.6825708652784797No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328940.3075710405979No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140780.1316344959426411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3750.036.732583
CGCGTAG2750.024.18188954-55
CAACGCA79700.021.75208712-13
TCGCGTA3150.021.11240452-53
ATCAACG83500.020.79061310-11
CTCGCGT3500.019.67977752-53
AGTGGTA104700.018.5547545
ACGTACG3850.018.5054220-21
GGTATCA104150.018.3786758
CGTACGC3650.018.21812220-21
GTATCAA107100.018.0941899
ACGCAGA97200.017.9335914-15
TCAACGC79300.017.90871210-11
ACTCGCG3800.017.50176850-51
AACGCAG82000.017.3769612-13
TGGTATC115600.016.7226477
GTGGTAT120450.016.3650686
CGTAGCG4250.016.2051756-57
AAGCAGT134250.015.5546671
CGCTAGT4500.015.3049990-91
Produced by FastQC (version 0.11.5)