FastQCFastQC Report
Sat 18 Jun 2016
SRR578555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10828269
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152210914.056808156502207No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT375120.34642656180780146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC355390.32820573630004946No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAGCACTCGGCCAGCTTC167980.15513098169245704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146620.13540483709815485No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG131900.12181078988710015No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT116960.10801357077479329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA3339550.091.708545
GGTATCA3357400.091.477678
GTATCAA3364550.091.3776259
TGGTATC3355600.091.349147
GTGGTAT3362000.091.278556
AAGCAGT3378650.091.138011
AGCAGTG3392700.090.687182
GCAGTGG3388550.090.619863
CAGTGGT3392000.090.263314
GTATCGA8000.046.923059
TCAACGC3356250.046.3995612-13
AACGCAG3364700.046.3095414-15
CAACGCA3354400.046.2119812-13
ATCAACG3355450.046.1840110-11
CGCAGAG3372550.046.18311316-17
ACGCAGA3368450.046.06827514-15
TATCAAC3377800.045.99025710-11
CAGAGTA3348650.045.90022718-19
AGAGTAC3286350.045.77446718-19
GAGTACT1933000.045.76906620-21
Produced by FastQC (version 0.11.5)