##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578555_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10828269 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.558300315590607 31.0 31.0 34.0 27.0 34.0 2 30.857723612148902 31.0 31.0 34.0 27.0 34.0 3 30.96332913413954 31.0 31.0 34.0 27.0 34.0 4 34.398188851791545 37.0 35.0 37.0 30.0 37.0 5 34.432617623370824 37.0 35.0 37.0 30.0 37.0 6 34.435315469166866 37.0 35.0 37.0 30.0 37.0 7 34.31960805554424 37.0 35.0 37.0 30.0 37.0 8 34.288080024609656 37.0 35.0 37.0 30.0 37.0 9 35.722592687713984 39.0 35.0 39.0 30.0 39.0 10-11 35.67633889590294 39.0 35.0 39.0 30.0 39.0 12-13 35.762796435884624 39.0 35.0 39.0 30.0 39.0 14-15 36.80197305774358 40.0 36.0 41.0 30.0 41.0 16-17 36.76497245312247 40.0 36.0 41.0 30.0 41.0 18-19 36.73403528301708 40.0 36.0 41.0 30.0 41.0 20-21 36.60899803098722 39.5 36.0 41.0 30.0 41.0 22-23 36.29227497026533 39.0 36.0 40.5 28.5 41.0 24-25 36.187477703038226 39.0 36.0 40.0 29.0 41.0 26-27 36.152684653475085 39.0 36.0 40.5 28.5 41.0 28-29 35.868699789412325 39.0 35.0 41.0 27.0 41.0 30-31 35.5893778590096 39.0 34.5 40.5 26.5 41.0 32-33 35.212193241597525 38.0 34.0 40.0 25.0 41.0 34-35 35.031205680243076 38.0 34.0 40.0 25.0 41.0 36-37 35.02729868458199 38.0 34.0 40.0 25.0 41.0 38-39 34.26111015527967 37.0 33.5 40.0 24.5 41.0 40-41 34.21246789306767 37.0 33.0 40.0 24.0 41.0 42-43 34.017656238499434 37.0 33.0 40.0 23.5 41.0 44-45 33.8504656192047 37.0 33.0 40.0 23.0 41.0 46-47 33.64074368673331 37.0 33.0 40.0 22.5 41.0 48-49 33.245747635194505 36.0 33.0 40.0 19.5 41.0 50-51 31.91101213868994 35.5 31.0 39.0 12.0 40.5 52-53 31.428608672355665 35.5 31.0 39.0 5.0 40.0 54-55 30.983442505907455 36.0 29.5 40.0 2.0 41.0 56-57 30.41378506573858 36.0 28.0 40.0 2.0 41.0 58-59 30.07879897516399 36.0 28.0 40.0 2.0 41.0 60-61 29.77846791578599 35.0 27.5 39.0 2.0 41.0 62-63 29.439840430635773 35.0 27.0 39.0 2.0 41.0 64-65 29.10537949325049 35.0 26.0 39.0 2.0 40.5 66-67 28.794052447348694 35.0 26.0 38.0 2.0 40.0 68-69 28.458669986864937 35.0 26.0 38.0 2.0 40.0 70-71 28.070542992605745 34.5 26.0 37.0 2.0 39.5 72-73 27.686132566525636 34.0 25.0 36.5 2.0 39.0 74-75 27.298680149154034 34.0 24.0 36.0 2.0 39.0 76-77 26.89631883914225 34.0 22.5 35.5 2.0 38.5 78-79 26.51691045909554 34.0 21.5 35.0 2.0 37.0 80-81 26.14070563817726 33.5 20.0 35.0 2.0 37.0 82-83 25.780075097875752 33.0 19.5 35.0 2.0 36.5 84-85 25.380950547128077 33.0 17.5 35.0 2.0 36.0 86-87 25.027058387633332 33.0 13.0 35.0 2.0 36.0 88-89 24.64643804102022 32.0 7.0 35.0 2.0 35.0 90-91 24.27305199935465 32.0 4.0 35.0 2.0 35.0 92-93 23.90945080880425 32.0 2.0 35.0 2.0 35.0 94-95 23.46946921987254 31.5 2.0 35.0 2.0 35.0 96-97 22.993852387671566 31.0 2.0 34.5 2.0 35.0 98-99 22.403388066920023 31.0 2.0 34.0 2.0 35.0 100-101 20.86308296367591 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4881367513025836 1101 2 0.5417630111282072 1101 3 0.5400503971044905 1101 4 0.6064785676012363 1101 5 0.5926420575594591 1101 6 0.48002048784591267 1101 7 0.4648193194002417 1101 8 0.6046574648783718 1101 9 0.5790707109896402 1101 10-11 0.6180556085027078 1101 12-13 0.6378449180619015 1101 14-15 0.796213736352783 1101 16-17 0.6498943029363033 1101 18-19 0.6534859424553403 1101 20-21 0.6371405655027971 1101 22-23 0.7444401079889928 1101 24-25 0.8900199746063322 1101 26-27 0.8101559093300352 1101 28-29 0.8184918003257948 1101 30-31 1.0131120806724496 1101 32-33 1.0512728167527854 1101 34-35 1.0065488484755178 1101 36-37 0.8297455171700889 1101 38-39 0.9401795224102898 1101 40-41 0.9027102777870724 1101 42-43 1.1980533925056989 1101 44-45 1.0723043143905642 1101 46-47 0.9982809036548659 1101 48-49 1.109893993302748 1101 50-51 1.1561963585977857 1101 52-53 1.1117205083356758 1101 54-55 0.7923880259911833 1101 56-57 0.7194111175423252 1101 58-59 0.7718760873019086 1101 60-61 0.9421198206726338 1101 62-63 0.8653382317036318 1101 64-65 0.8876544563436433 1101 66-67 0.8109587211707492 1101 68-69 0.85016759961416 1101 70-71 0.85929795086507 1101 72-73 0.9525396765981284 1101 74-75 0.9393416627825957 1101 76-77 0.8673652757810011 1101 78-79 0.9100425262389926 1101 80-81 1.0037356903655912 1101 82-83 1.0900265070642376 1101 84-85 1.1977475576072898 1101 86-87 1.1520022057835675 1101 88-89 1.2592744790058887 1101 90-91 1.3467488735591466 1101 92-93 1.4315156267492348 1101 94-95 1.4589230052621787 1101 96-97 1.671744319673948 1101 98-99 1.880025013592327 1101 100-101 2.2742833274170025 1102 1 0.44389382975179714 1102 2 0.39904047843640456 1102 3 0.36940330187011483 1102 4 0.4510481026357809 1102 5 0.39286145967669484 1102 6 0.2879216563458513 1102 7 0.4111005870608295 1102 8 0.4939797219074151 1102 9 0.41833205719495226 1102 10-11 0.5188482688903875 1102 12-13 0.5514479772804037 1102 14-15 0.6844060623486925 1102 16-17 0.6075385226829226 1102 18-19 0.585789015073928 1102 20-21 0.6119083890526085 1102 22-23 0.5950180244360439 1102 24-25 0.7604287684547373 1102 26-27 0.7819961055369617 1102 28-29 0.6729846509776678 1102 30-31 0.776215344864049 1102 32-33 0.8243195886577226 1102 34-35 0.8590439477938361 1102 36-37 0.6879418114793125 1102 38-39 0.7402698683785971 1102 40-41 0.7898007417625266 1102 42-43 0.852951079355158 1102 44-45 0.8486072117177699 1102 46-47 0.7891572963330447 1102 48-49 0.9744151184868457 1102 50-51 0.922135619333563 1102 52-53 0.8823451883442637 1102 54-55 0.7875468943370905 1102 56-57 0.6337129965481374 1102 58-59 0.6874678347349636 1102 60-61 0.6672724869129603 1102 62-63 0.6680843914135437 1102 64-65 0.7004351947847205 1102 66-67 0.6597205884920712 1102 68-69 0.7520430494880621 1102 70-71 0.6379578988816874 1102 72-73 0.7056523848262835 1102 74-75 0.8594344216730505 1102 76-77 0.6820322358794719 1102 78-79 0.692183444997756 1102 80-81 0.8422865146975802 1102 82-83 0.9953389982093732 1102 84-85 0.989519959551604 1102 86-87 1.006032304241824 1102 88-89 1.1954679087830655 1102 90-91 1.1506513273159804 1102 92-93 1.1154762038875887 1102 94-95 1.139592537313888 1102 96-97 1.3343756326061467 1102 98-99 1.430619062391198 1102 100-101 1.810027562360343 1103 1 0.3425460353514893 1103 2 0.28064096222522394 1103 3 0.19684981297129056 1103 4 0.3412493733242883 1103 5 0.2546187651511147 1103 6 0.16047174603802716 1103 7 0.38468351728163697 1103 8 0.3141195952050353 1103 9 0.5217094366812276 1103 10-11 0.5134062039446761 1103 12-13 0.33479712533512895 1103 14-15 0.5910498915723181 1103 16-17 0.47989987144747914 1103 18-19 0.4914797643042377 1103 20-21 0.5890435841419261 1103 22-23 0.5912173852332074 1103 24-25 0.500447923722767 1103 26-27 0.6357132797437757 1103 28-29 0.6359825533145269 1103 30-31 0.5888162585444903 1103 32-33 0.7888329129884966 1103 34-35 0.7535298411844522 1103 36-37 0.7963439501429832 1103 38-39 0.6039422438424822 1103 40-41 0.6628647085045074 1103 42-43 0.48861930817971455 1103 44-45 0.6553784603860606 1103 46-47 0.791255774910141 1103 48-49 0.7263573304710889 1103 50-51 0.6430662227239914 1103 52-53 0.7132536816928372 1103 54-55 0.699287497078334 1103 56-57 0.612343368670416 1103 58-59 0.7240241077898055 1103 60-61 0.6699685974204925 1103 62-63 0.7555284743148043 1103 64-65 0.7348173972593806 1103 66-67 0.6950884456230639 1103 68-69 0.7992607962874345 1103 70-71 0.850221067526487 1103 72-73 0.7844458112814863 1103 74-75 0.9846489885876153 1103 76-77 1.0248333928870998 1103 78-79 1.0802042454442322 1103 80-81 0.9562779574915687 1103 82-83 1.11617867260858 1103 84-85 1.0492791394652095 1103 86-87 1.2187424544718155 1103 88-89 1.2887911718920009 1103 90-91 1.1374975289604201 1103 92-93 1.2139803838999406 1103 94-95 1.259687421714517 1103 96-97 1.4118310088826505 1103 98-99 1.5599379087685996 1103 100-101 1.7733068235461502 1104 1 0.17661656451190666 1104 2 0.20963762765232374 1104 3 0.17624257549239886 1104 4 0.20862625399432488 1104 5 0.039011154883304755 1104 6 0.05356305153648577 1104 7 0.2546808766760691 1104 8 0.19449185174873662 1104 9 0.2910985389032916 1104 10-11 0.3948117304079446 1104 12-13 0.24951053034490656 1104 14-15 0.42314365897964734 1104 16-17 0.5741156643715968 1104 18-19 0.6292239686881942 1104 20-21 0.46104618494440075 1104 22-23 0.41206134680578543 1104 24-25 0.3842879308122207 1104 26-27 0.4417198709522907 1104 28-29 0.671510418045095 1104 30-31 0.5707403934332476 1104 32-33 0.7566602027813332 1104 34-35 0.720083344405225 1104 36-37 0.7899028919097688 1104 38-39 0.6043579608570155 1104 40-41 0.6935034308970032 1104 42-43 0.6878396515485576 1104 44-45 0.9442934368502875 1104 46-47 0.7647835058789525 1104 48-49 0.688277799636893 1104 50-51 0.7529752067367106 1104 52-53 0.6208581918254588 1104 54-55 0.668222789808592 1104 56-57 0.600370706969624 1104 58-59 0.6802185349646912 1104 60-61 0.6160804256687307 1104 62-63 0.5416555111827748 1104 64-65 0.8038841236390333 1104 66-67 0.6082169198666492 1104 68-69 0.6920819748035925 1104 70-71 0.8187475274248506 1104 72-73 0.6369616692048048 1104 74-75 0.6612343146727788 1104 76-77 0.7549322493325761 1104 78-79 0.8664935112052667 1104 80-81 0.7458823034885853 1104 82-83 0.819379571374089 1104 84-85 0.7593225736509837 1104 86-87 0.7745541502975861 1104 88-89 0.9298457126842692 1104 90-91 1.0323909424122881 1104 92-93 1.0077752570363465 1104 94-95 1.0238212640768332 1104 96-97 1.2435433925717803 1104 98-99 1.4743610390882225 1104 100-101 1.763486178199507 1105 1 -0.04856788427451875 1105 2 0.16731992208761426 1105 3 0.17393134361261176 1105 4 0.19756199582681688 1105 5 0.24685632624296971 1105 6 0.30982953925339274 1105 7 0.3609355441668498 1105 8 0.3879132124546132 1105 9 0.25114852365667417 1105 10-11 0.35538653158129563 1105 12-13 0.29121614381625704 1105 14-15 0.13707473813085613 1105 16-17 0.3465332061946853 1105 18-19 0.1621499669444617 1105 20-21 0.24211803989044967 1105 22-23 0.3640637441428609 1105 24-25 0.534606918963604 1105 26-27 0.497836602054079 1105 28-29 0.6191714895347147 1105 30-31 0.5122440728102333 1105 32-33 0.6096564306367327 1105 34-35 0.5031734832180632 1105 36-37 0.7964441892525898 1105 38-39 0.5743231733057854 1105 40-41 0.50735507942467 1105 42-43 0.8073961174593052 1105 44-45 0.8161796496875056 1105 46-47 0.5656894856092407 1105 48-49 0.7886903633971656 1105 50-51 0.7401576548531672 1105 52-53 0.6310316986506272 1105 54-55 0.7142914579309441 1105 56-57 0.6745442236770494 1105 58-59 0.7159568195653598 1105 60-61 0.7212698674223539 1105 62-63 0.8168317038338699 1105 64-65 0.9030047571367312 1105 66-67 0.7463155640986585 1105 68-69 0.7750205020540477 1105 70-71 0.739787821536865 1105 72-73 0.6708133023420864 1105 74-75 0.6964674744551402 1105 76-77 0.5973714174916047 1105 78-79 0.7812541365395766 1105 80-81 0.850592601835789 1105 82-83 0.9276983455990191 1105 84-85 0.9461380540687578 1105 86-87 0.9954154906006636 1105 88-89 1.0082255045486548 1105 90-91 1.115431042200349 1105 92-93 1.1368246909351996 1105 94-95 1.2218199946934973 1105 96-97 1.4164750139913025 1105 98-99 1.645186072241355 1105 100-101 1.8761802059862127 1106 1 0.23949493043700798 1106 2 0.3127047752727137 1106 3 0.28111480753242546 1106 4 0.09640409651370874 1106 5 0.3182265300574869 1106 6 0.2850065050322428 1106 7 0.4189152575325963 1106 8 0.36578001948852545 1106 9 0.3757127607681028 1106 10-11 0.25416608610452585 1106 12-13 0.3930988253682983 1106 14-15 0.30605602510475904 1106 16-17 0.42446072707424065 1106 18-19 0.36852417788400516 1106 20-21 0.3082479542480243 1106 22-23 0.41542076055084465 1106 24-25 0.5267563327871443 1106 26-27 0.41592850435391426 1106 28-29 0.4929753967171564 1106 30-31 0.5378396023833218 1106 32-33 0.5009153282958039 1106 34-35 0.38185751209459795 1106 36-37 0.4767557055698717 1106 38-39 0.48823672210803437 1106 40-41 0.3760427888669682 1106 42-43 0.6045766915358257 1106 44-45 0.5408295518690167 1106 46-47 0.6101608285996534 1106 48-49 0.7106801000307854 1106 50-51 0.5196636844882541 1106 52-53 0.5543792717926372 1106 54-55 0.6940126484503217 1106 56-57 0.46843377767843464 1106 58-59 0.5811454940084637 1106 60-61 0.5315989643067418 1106 62-63 0.5858777391151264 1106 64-65 0.7268678800507153 1106 66-67 0.692430797338293 1106 68-69 0.6378864232274353 1106 70-71 0.6917949618296433 1106 72-73 0.6132969437005649 1106 74-75 0.5925880025255132 1106 76-77 0.6960695719471133 1106 78-79 0.7632247075589547 1106 80-81 0.8180100049778325 1106 82-83 0.984382720590741 1106 84-85 1.0499124899087242 1106 86-87 1.019262941552114 1106 88-89 1.1108705272568713 1106 90-91 1.0260919903697712 1106 92-93 1.157649945839335 1106 94-95 1.0546411835326452 1106 96-97 1.2568062452179944 1106 98-99 1.496900913382543 1106 100-101 1.7501199400458631 1107 1 0.13029778770958345 1107 2 0.16766517867345954 1107 3 0.24021690970947063 1107 4 0.1741602500651922 1107 5 0.25265152250077705 1107 6 0.2953776573666218 1107 7 0.2984201616143025 1107 8 0.31277995137944714 1107 9 0.20913014142817588 1107 10-11 0.2624889337691698 1107 12-13 0.33524719628672983 1107 14-15 0.40034381368788985 1107 16-17 0.319359336622135 1107 18-19 0.3029009860239711 1107 20-21 0.3499259966720132 1107 22-23 0.5267031010209777 1107 24-25 0.5070319883614047 1107 26-27 0.4925376190848141 1107 28-29 0.6286425644596889 1107 30-31 0.664664160236903 1107 32-33 0.6105631845432811 1107 34-35 0.615025715901865 1107 36-37 0.5333933976499452 1107 38-39 0.6341932161482973 1107 40-41 0.44075832228018186 1107 42-43 0.5054103995005477 1107 44-45 0.5212246184945926 1107 46-47 0.6484949251918479 1107 48-49 0.6505550375457005 1107 50-51 0.6215383503216962 1107 52-53 0.6479980235257408 1107 54-55 0.7763698132462871 1107 56-57 0.5144425573491525 1107 58-59 0.583082407860136 1107 60-61 0.6128173817879663 1107 62-63 0.6929493667017894 1107 64-65 0.7502334378949733 1107 66-67 0.7773489130370166 1107 68-69 0.7817144456488023 1107 70-71 0.8090521126656824 1107 72-73 0.8173742478667982 1107 74-75 0.8004039895332369 1107 76-77 0.8128823507514547 1107 78-79 0.8189879111470866 1107 80-81 0.8637367004641838 1107 82-83 0.8808713649704991 1107 84-85 1.0125056974033377 1107 86-87 1.059970932832325 1107 88-89 1.1113060888264492 1107 90-91 1.1119495584497976 1107 92-93 1.1368825425582756 1107 94-95 1.125189537731245 1107 96-97 1.246165082295498 1107 98-99 1.4294169330514883 1107 100-101 1.6748140935615865 1108 1 0.15937044305674064 1108 2 0.1975512237145729 1108 3 0.004291934400093567 1108 4 0.17607687534899696 1108 5 0.24740944706718437 1108 6 0.26103798324127325 1108 7 0.20926328349290912 1108 8 0.15685854007669775 1108 9 0.1358530678882488 1108 10-11 0.17446806317199304 1108 12-13 0.2796318797096049 1108 14-15 0.21467318918193 1108 16-17 0.27378146719125596 1108 18-19 0.05243666601691643 1108 20-21 0.1382230503559967 1108 22-23 0.2971562086694206 1108 24-25 0.3846867912795915 1108 26-27 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86-87 -1.6743855046190035 2315 88-89 -1.9311220444359947 2315 90-91 -1.98234457435316 2315 92-93 -1.9216888483608905 2315 94-95 -2.1243586786452084 2315 96-97 -2.1128968022581134 2315 98-99 -2.2592843527810693 2315 100-101 -2.273371246544702 2316 1 -1.198014124833204 2316 2 -1.0038342778201361 2316 3 -2.2782247976670718 2316 4 -1.3165503661850053 2316 5 -1.1440144711009097 2316 6 -2.6051771572853184 2316 7 -6.703879242506574 2316 8 -7.436544322521314 2316 9 -4.106431382946951 2316 10-11 -2.044400670587571 2316 12-13 -2.0052258916194035 2316 14-15 -2.1190900987013563 2316 16-17 -1.9903240099096706 2316 18-19 -1.5565090807395947 2316 20-21 -2.5724564499718596 2316 22-23 -3.2350651529022088 2316 24-25 -1.969650473092841 2316 26-27 -2.2134094771570574 2316 28-29 -2.9208643396919456 2316 30-31 -3.504851685748669 2316 32-33 -3.469678365176307 2316 34-35 -2.5999769978098044 2316 36-37 -3.7190362332803915 2316 38-39 -3.24856231319184 2316 40-41 -4.05310285327063 2316 42-43 -3.3479597033353485 2316 44-45 -2.9939660621480293 2316 46-47 -2.6987450691159047 2316 48-49 -2.6779105871374007 2316 50-51 -2.456074429561177 2316 52-53 -2.8548669977903423 2316 54-55 -4.3131020898459695 2316 56-57 -3.0112046960849277 2316 58-59 -2.5098187434496246 2316 60-61 -3.163768883152432 2316 62-63 -2.970842045088844 2316 64-65 -4.402092189608414 2316 66-67 -3.403842431039614 2316 68-69 -3.270464870960918 2316 70-71 -3.636205920581734 2316 72-73 -3.246161846908734 2316 74-75 -2.98578330945805 2316 76-77 -3.4639666285121855 2316 78-79 -4.574247522713502 2316 80-81 -3.517227728763192 2316 82-83 -3.228571505636882 2316 84-85 -3.403473146689244 2316 86-87 -4.424273029271461 2316 88-89 -4.816658221914814 2316 90-91 -4.27015992933557 2316 92-93 -4.341622869733303 2316 94-95 -4.509318573100863 2316 96-97 -4.666784201378675 2316 98-99 -4.786021927239705 2316 100-101 -4.829065708302409 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 136255.0 3 22500.0 4 19041.0 5 17740.0 6 19727.0 7 23205.0 8 29130.0 9 34703.0 10 40156.0 11 48256.0 12 50781.0 13 57662.0 14 66343.0 15 79130.0 16 100705.0 17 136049.0 18 196951.0 19 291825.0 20 380545.0 21 272483.0 22 157640.0 23 138947.0 24 145032.0 25 156643.0 26 172268.0 27 194787.0 28 223781.0 29 262517.0 30 308403.0 31 367794.0 32 444620.0 33 550469.0 34 704036.0 35 971758.0 36 1389079.0 37 1458041.0 38 1012605.0 39 146548.0 40 114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.074382110863779 49.96116492645469 23.345399329040887 16.619053633640643 2 15.872593817629385 51.724063968616264 17.199775450098294 15.20356676365605 3 46.81575837462144 19.703709305375295 18.058114185822767 15.422418134180504 4 15.958890801113762 18.083558742309428 18.987376172963234 46.970174283613574 5 15.875852052181857 49.18083371835027 19.987814422022563 14.955499807445314 6 47.05315418023184 19.190897583954676 17.9148541504872 15.841094085326276 7 16.766189854504823 18.604077170597538 49.828758243283005 14.800974731614632 8 46.62878001818226 19.359294746324146 18.791669805656838 15.220255429836751 9 46.2975999296038 19.009506259771218 19.30164678670377 15.391247023921212 10-11 15.58272634597771 34.29518763233803 34.40285568257542 15.719230339108838 12-13 15.365394119029208 19.351699131691223 34.37335544517604 30.909551304103527 14-15 15.567696978860788 49.864337832577256 18.815173940738042 15.752791247823916 16-17 30.92241685589492 19.151262140076454 18.92174852125647 31.004572482772154 18-19 15.69524312744511 34.371079499689586 18.953869770165497 30.97980760269981 20-21 31.110155046485023 34.44304438266678 18.954201439705813 15.49259913114239 22-23 31.046399978711875 19.111630152853163 34.27229912474767 15.569670743687295 24-25 15.636610445608278 34.199431999399806 19.501829768346372 30.662127786645538 26-27 15.866321292016428 25.065457605936135 43.199816401341565 15.868404700705868 28-29 28.032253853182116 18.98461073937801 37.27354814282152 15.709587264618358 30-31 25.661153949749337 20.237132730038145 37.75170910976905 16.350004210443462 32-33 19.479893224862412 22.948874803467064 39.26539219064632 18.305839781024208 34-35 18.625398344815007 21.80897809946144 40.00889315065228 19.556730405071278 36-37 18.572239685258797 21.849160018701156 40.29014973860883 19.288450557431215 38-39 18.791506360703167 22.2738007145838 39.8733731303759 19.061319794337134 40-41 18.975528841542776 21.8763981136542 39.83389693695185 19.314176107851168 42-43 19.51611774366603 22.205646032151176 39.817658316076624 18.460577908106167 44-45 18.702986669194587 22.402190235779365 39.695829125907885 19.198993969118167 46-47 18.943494997943297 22.081921082479887 39.60149117428682 19.373092745290002 48-49 19.18464655061979 22.235509543010153 39.658188369438626 18.92165553693144 50-51 19.316588887318677 22.108967344315687 38.85276505016178 19.72167871820386 52-53 19.53032913898942 23.11285359965641 37.834979027510485 19.521838233843685 54-55 20.205127314364614 24.08108430984299 36.04278980021577 19.670998575576622 56-57 20.544373064966496 24.612954031695246 34.92666211752808 19.91601078581018 58-59 20.564344396325378 25.61352352456725 33.81230396848426 20.009828110623115 60-61 20.738923158220587 25.90469980880018 33.19977510034076 20.156601932638477 62-63 20.59692455276258 25.755245530305388 33.62973986612272 20.018090050809313 64-65 20.388506273996715 25.70855926686637 33.43620563144177 20.466728827695146 66-67 20.526164194697245 25.082827702349213 34.269943079990824 20.12106502296272 68-69 20.212273493368414 24.801546780477878 34.61754208156167 20.36863764459203 70-71 20.090167008125768 24.573140705649905 35.29803172739991 20.038660558824418 72-73 19.803503054041443 24.36872934303208 35.026276554768145 20.801491048158333 74-75 20.325789535757856 24.564799121229726 34.43530403578611 20.674107307226308 76-77 20.795425238251134 25.104564511209325 33.8290532441849 20.270957006354642 78-79 20.4971963823985 24.998072436385435 33.73196134229016 20.7727698389259 80-81 20.338634375982135 25.446372164913257 33.57062298327012 20.64437047583449 82-83 21.33563768714175 25.646517397951325 32.765890673335214 20.251954241571717 84-85 20.872651250131895 26.278016700948285 32.26161769947939 20.58771434944043 86-87 20.83692567201411 26.082577883488756 32.36497187742803 20.7155245670691 88-89 20.736871501024527 25.856037334416186 32.17713798271916 21.229953181840123 90-91 20.736698726195684 25.79768561988721 32.23555138487982 21.23006426903729 92-93 20.756575277699568 26.161831989133375 32.316055865810824 20.765536867356232 94-95 20.94579019263008 25.870794315567053 31.774567507376243 21.408847984426615 96-97 20.75501547100002 25.776059255068656 32.54832444420775 20.920600829723572 98-99 20.867866710621072 26.17210939621492 31.7158657158732 21.244158177290807 100-101 21.414556812211696 25.907329211193286 31.43029022406198 21.247823752533037 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17044.0 1 10327.5 2 2661.5 3 1622.5 4 1385.5 5 1144.5 6 1279.0 7 1582.5 8 1493.0 9 1517.0 10 2023.5 11 13772.5 12 254903.5 13 284309.5 14 73377.5 15 64127.0 16 68277.5 17 73519.0 18 77354.5 19 79542.0 20 80568.0 21 81203.5 22 82994.5 23 85806.0 24 89776.0 25 95494.5 26 102394.0 27 110431.0 28 119704.0 29 128292.5 30 136763.5 31 146969.5 32 158437.0 33 172273.5 34 196653.5 35 213717.0 36 220800.5 37 234382.5 38 246175.0 39 256228.5 40 268694.5 41 277769.5 42 284436.5 43 298397.0 44 317451.0 45 326453.5 46 326090.5 47 332801.5 48 343970.0 49 351328.0 50 352810.0 51 348405.0 52 344329.5 53 345793.0 54 328468.0 55 301583.5 56 299845.0 57 285973.5 58 265284.5 59 225519.0 60 164155.5 61 125870.5 62 94157.5 63 67286.5 64 46550.0 65 30273.5 66 19304.5 67 12293.0 68 8325.5 69 5911.5 70 4613.0 71 3999.5 72 3631.5 73 3327.0 74 3071.0 75 2889.5 76 2742.5 77 2618.5 78 2446.0 79 2263.0 80 2161.5 81 1963.5 82 1747.0 83 1634.0 84 1497.5 85 1327.0 86 1115.5 87 919.5 88 777.5 89 626.5 90 453.5 91 341.0 92 269.0 93 176.5 94 112.5 95 86.5 96 58.5 97 27.5 98 18.5 99 12.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12285435465262269 2 0.11049780902192216 3 0.10520610450294501 4 0.09378230260072039 5 0.09755021786030621 6 0.08787184729156618 7 0.09717157931706351 8 0.09266485714383342 9 0.08776102625451954 10-11 0.07793489430305066 12-13 0.06946170251219286 14-15 0.07736231894497633 16-17 0.06780862204291378 18-19 0.060466728338573784 20-21 0.05658799204194132 22-23 0.049250715880811605 24-25 0.04655407064601 26-27 0.0430401202629894 28-29 0.0456397970903752 30-31 0.036880317620480246 32-33 0.0492922737697041 34-35 0.05963557056072397 36-37 0.049952582448773666 38-39 0.05176727693041243 40-41 0.05218747336254761 42-43 0.04785160028809776 44-45 0.047486814374485896 46-47 0.04729287755965427 48-49 0.04696503199172462 50-51 0.04836876512764875 52-53 0.04546894799159496 54-55 0.0456397970903752 56-57 0.045939937399043194 58-59 0.04832258969554598 60-61 0.04410215520135305 62-63 0.05306018902928991 64-65 0.04897828083140528 66-67 0.043834337695157 68-69 0.04152556609001864 70-71 0.0494308000660124 72-73 0.04379277980626451 74-75 0.04582911636199655 76-77 0.04108228194183207 78-79 0.04636475137438865 80-81 0.048110182707873254 82-83 0.038057791139100815 84-85 0.04775463188068194 86-87 0.050497452547586315 88-89 0.046281635596603664 90-91 0.04201964321351825 92-93 0.039678548805907946 94-95 0.04316479392966687 96-97 0.030831336015017727 98-99 0.03154243766940034 100-101 0.02384037559465876 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0828269E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.355659011810374 #Duplication Level Percentage of deduplicated Percentage of total 1 90.71883505677765 36.61018373499988 2 5.105272923938405 4.120533065613728 3 1.4274055085050301 1.7281166991842636 4 0.6944665709706415 1.1210262453276965 5 0.4065670692334827 0.8203641005708763 6 0.2395480294847526 0.5800271156902663 7 0.17533238057378012 0.4952957634915105 8 0.13845896484878187 0.44700822180525435 9 0.10445315021187966 0.3793748141394022 >10 0.9239832384812117 7.472637851814369 >50 0.04061659842923736 1.0681775255701818 >100 0.01576304676692058 1.2886652113739268 >500 0.0038186003732182815 1.0897030941478882 >1k 0.004372020717107458 3.714419784011755 >5k 6.641044127251835E-4 1.9386271864328075 >10k+ 4.4273627515012236E-4 37.12583958582625 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1522109 14.056808156502207 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 37512 0.34642656180780146 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCAGCAGCTGACCCAGC 35539 0.32820573630004946 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGACAGCACTCGGCCAGCTTC 16798 0.15513098169245704 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14662 0.13540483709815485 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 13190 0.12181078988710015 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 11696 0.10801357077479329 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.541051852332076E-5 0.0 0.0 0.0 0.0 2 9.23508642055346E-5 1.847017284110692E-5 0.0 0.0 0.0 3 1.2929120988774845E-4 1.847017284110692E-5 0.0 0.0 0.0 4 1.7546664199051575E-4 1.847017284110692E-5 0.0 0.0 0.0 5 1.9393681483162266E-4 1.847017284110692E-5 0.0 0.0 0.0 6 2.0317190125217614E-4 1.847017284110692E-5 0.0 0.0 9.23508642055346E-6 7 2.2164207409328305E-4 1.847017284110692E-5 0.0 0.0 9.23508642055346E-6 8 2.4011224693438996E-4 2.770525926166038E-5 0.0 0.0 9.23508642055346E-6 9 2.955227654577107E-4 2.770525926166038E-5 0.0 0.0 9.23508642055346E-6 10-11 3.9249117287352205E-4 2.770525926166038E-5 0.0 0.0 9.23508642055346E-6 12-13 4.8022449386878E-4 2.770525926166038E-5 0.0 0.0 9.23508642055346E-6 14-15 5.217823827612705E-4 2.770525926166038E-5 0.0 9.23508642055346E-6 1.847017284110692E-5 16-17 5.541051852332076E-4 3.694034568221384E-5 0.0 9.23508642055346E-6 2.770525926166038E-5 18-19 6.002806173359749E-4 3.694034568221384E-5 0.0 9.23508642055346E-6 2.770525926166038E-5 20-21 6.187507901770819E-4 4.61754321027673E-5 0.0 9.23508642055346E-6 2.770525926166038E-5 22-23 6.372209630181888E-4 5.079297531304403E-5 0.0 9.23508642055346E-6 2.770525926166038E-5 24-25 7.111016543826165E-4 5.541051852332076E-5 0.0 9.23508642055346E-6 3.694034568221384E-5 26-27 7.757472593264906E-4 5.541051852332076E-5 0.0 9.23508642055346E-6 3.694034568221384E-5 28-29 8.080700617984278E-4 5.541051852332076E-5 0.0 9.23508642055346E-6 3.694034568221384E-5 30-31 8.634805803217486E-4 6.00280617335975E-5 0.0 9.23508642055346E-6 4.1557888892490574E-5 32-33 9.373612716861762E-4 6.464560494387422E-5 0.0 9.23508642055346E-6 4.61754321027673E-5 34-35 0.001043564765522541 6.926314815415095E-5 0.0 9.23508642055346E-6 4.61754321027673E-5 36-37 0.0011174454568869687 7.388069136442768E-5 0.0 9.23508642055346E-6 4.61754321027673E-5 38-39 0.0012005612346719498 7.388069136442768E-5 0.0 9.23508642055346E-6 4.61754321027673E-5 40-41 0.0012790594692466545 7.388069136442768E-5 0.0 9.23508642055346E-6 4.61754321027673E-5 42-43 0.0013575577038213587 7.849823457470442E-5 0.0 1.3852629630830189E-5 5.541051852332076E-5 44-45 0.0015099366297604906 1.0158595062608807E-4 0.0 1.847017284110692E-5 5.541051852332076E-5 46-47 0.0016022874939660256 1.0158595062608807E-4 0.0 2.770525926166038E-5 5.541051852332076E-5 48-49 0.0016346102964379626 1.0158595062608807E-4 0.0 2.770525926166038E-5 5.541051852332076E-5 50-51 0.0017592839631154341 1.1543858025691825E-4 0.0 2.770525926166038E-5 6.00280617335975E-5 52-53 0.0018885751730031825 1.2929120988774845E-4 0.0 2.770525926166038E-5 6.464560494387422E-5 54-55 0.0020409540989423146 1.2929120988774845E-4 0.0 2.770525926166038E-5 6.464560494387422E-5 56-57 0.0021517751359889565 1.477613827288554E-4 0.0 3.694034568221384E-5 6.464560494387422E-5 58-59 0.0022533610866150443 1.5699646914940884E-4 0.0 3.694034568221384E-5 6.464560494387422E-5 60-61 0.0023687996668719626 1.5699646914940884E-4 0.0 3.694034568221384E-5 6.464560494387422E-5 62-63 0.002604294370596076 1.5699646914940884E-4 0.0 3.694034568221384E-5 6.464560494387422E-5 64-65 0.0027843785557968683 1.6161401235968557E-4 0.0 3.694034568221384E-5 6.464560494387422E-5 66-67 0.0030198732595209813 1.7546664199051575E-4 0.0 3.694034568221384E-5 6.464560494387422E-5 68-69 0.003269220592875925 1.7546664199051575E-4 0.0 3.694034568221384E-5 6.464560494387422E-5 70-71 0.0035878310743850195 1.847017284110692E-4 0.0 4.1557888892490574E-5 6.926314815415095E-5 72-73 0.003920294185524944 1.9393681483162266E-4 0.0 5.541051852332076E-5 7.388069136442768E-5 74-75 0.004271227469505976 2.0317190125217614E-4 0.0 6.464560494387422E-5 7.388069136442768E-5 76-77 0.004719129160902818 2.124069876727296E-4 0.0 7.849823457470442E-5 7.849823457470442E-5 78-79 0.005194736111561321 2.124069876727296E-4 0.0 8.311577778498115E-5 8.311577778498115E-5 80-81 0.005781164099266467 2.124069876727296E-4 0.0 8.311577778498115E-5 8.311577778498115E-5 82-83 0.006469178037597698 2.124069876727296E-4 0.0 9.23508642055346E-5 9.23508642055346E-5 84-85 0.007461949827807196 2.124069876727296E-4 0.0 1.062034938363648E-4 9.23508642055346E-5 86-87 0.008819507531628554 2.1702453088300633E-4 0.0 1.1082103704664153E-4 9.23508642055346E-5 88-89 0.010841991457729763 2.308771605138365E-4 0.0 1.1082103704664153E-4 9.23508642055346E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 333955 0.0 91.70854 5 GGTATCA 335740 0.0 91.47767 8 GTATCAA 336455 0.0 91.377625 9 TGGTATC 335560 0.0 91.34914 7 GTGGTAT 336200 0.0 91.27855 6 AAGCAGT 337865 0.0 91.13801 1 AGCAGTG 339270 0.0 90.68718 2 GCAGTGG 338855 0.0 90.61986 3 CAGTGGT 339200 0.0 90.26331 4 GTATCGA 800 0.0 46.92305 9 TCAACGC 335625 0.0 46.39956 12-13 AACGCAG 336470 0.0 46.30954 14-15 CAACGCA 335440 0.0 46.21198 12-13 ATCAACG 335545 0.0 46.18401 10-11 CGCAGAG 337255 0.0 46.183113 16-17 ACGCAGA 336845 0.0 46.068275 14-15 TATCAAC 337780 0.0 45.990257 10-11 CAGAGTA 334865 0.0 45.900227 18-19 AGAGTAC 328635 0.0 45.774467 18-19 GAGTACT 193300 0.0 45.769066 20-21 >>END_MODULE