FastQCFastQC Report
Sat 18 Jun 2016
SRR578555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10828269
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334400.3088212899033077No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT142720.13180315339413898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4750.031.984653
CGCGTAG3050.024.90671254-55
CGTACGC3150.024.11507620-21
TCGCGTA3600.021.76249952-53
CAACGCA83300.020.26132212-13
ACTCGCG3650.020.16444450-51
ATCAACG87000.019.4270110-11
CTCGCGT4050.018.7582552-53
ACGTACG4200.018.65150520-21
GGTATCA104450.017.999738
TCAACGC81700.017.86891710-11
AGTGGTA105950.017.7448965
GTATCAA108550.017.5385539
AACGCAG86700.016.86571712-13
ACGCAGA99850.016.78416814-15
TGGTATC115350.016.381167
GTGGTAT120250.015.83216
CGTAGCG4950.015.34599356-57
CGCTAGT4650.015.31530290-91
AAGCAGT138750.014.5644951
Produced by FastQC (version 0.11.5)