##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578555_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10828269 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.380327640549012 31.0 31.0 34.0 30.0 34.0 2 31.753616667631732 31.0 31.0 34.0 30.0 34.0 3 31.826476974297552 31.0 31.0 34.0 30.0 34.0 4 35.53794757038267 37.0 35.0 37.0 33.0 37.0 5 35.362898077245774 37.0 35.0 37.0 33.0 37.0 6 35.35451178761813 37.0 35.0 37.0 32.0 37.0 7 35.34816414331783 37.0 35.0 37.0 32.0 37.0 8 35.30394765774659 37.0 35.0 37.0 32.0 37.0 9 36.84320467103283 39.0 37.0 39.0 32.0 39.0 10-11 36.83702727555069 39.0 37.0 39.0 32.0 39.0 12-13 36.82917121840988 39.0 37.0 39.0 32.0 39.0 14-15 37.99806737346477 40.0 37.0 41.0 33.0 41.0 16-17 37.92138554186269 40.0 37.0 41.0 33.0 41.0 18-19 37.83150991169502 40.0 37.0 41.0 32.0 41.0 20-21 37.78063391295506 40.0 37.0 41.0 32.0 41.0 22-23 37.57335983249031 39.0 36.0 41.0 32.0 41.0 24-25 37.57226372008305 39.0 36.0 41.0 32.0 41.0 26-27 37.39861061818837 39.0 36.0 41.0 32.0 41.0 28-29 37.24714684313808 39.0 36.0 41.0 31.0 41.0 30-31 37.1086412795988 39.0 36.0 41.0 31.0 41.0 32-33 36.96854935908962 39.0 36.0 40.0 30.5 41.0 34-35 36.82137583578687 39.0 35.0 40.0 30.0 41.0 36-37 36.66817226280581 38.5 35.0 40.0 30.0 41.0 38-39 36.498078455568475 38.0 35.0 40.0 30.0 41.0 40-41 36.33772812625915 38.0 35.0 40.0 30.0 41.0 42-43 36.418094480290435 38.0 35.0 40.0 30.0 41.0 44-45 36.34977543502106 38.0 35.0 40.0 30.0 41.0 46-47 36.198486757209295 38.0 35.0 40.0 30.0 41.0 48-49 36.05020243771189 38.0 35.0 40.0 29.0 41.0 50-51 35.86371690618326 38.0 34.5 40.0 28.5 41.0 52-53 35.90203129419855 38.0 34.5 40.0 28.0 41.0 54-55 35.889691602600564 38.0 34.5 40.0 28.0 41.0 56-57 35.72696296148535 38.0 34.0 40.0 28.0 41.0 58-59 35.49940115082106 38.0 34.0 40.0 28.0 41.0 60-61 35.23593664878477 38.0 34.0 40.0 28.0 41.0 62-63 34.948553365270115 37.0 34.0 40.0 27.0 41.0 64-65 34.62155363890572 37.0 33.0 40.0 26.5 41.0 66-67 34.2733654843632 36.0 33.0 39.0 26.0 41.0 68-69 33.889189260074716 36.0 32.5 39.0 26.0 40.5 70-71 33.488715186148404 35.0 32.0 38.5 26.0 40.0 72-73 33.09340052412809 35.0 32.0 38.0 25.5 40.0 74-75 32.67793693525715 35.0 31.5 37.0 25.0 39.5 76-77 31.303762540439287 34.0 29.5 36.0 23.0 38.5 78-79 31.955123344275986 34.5 31.0 36.0 24.0 39.0 80-81 31.828539492323287 34.5 31.0 36.0 24.0 38.0 82-83 31.465153202233893 34.0 31.0 35.5 23.0 37.0 84-85 31.18375199212358 34.0 31.0 35.0 23.0 37.0 86-87 30.86701147708835 34.0 31.0 35.0 21.0 36.0 88-89 30.55875689826324 34.0 30.5 35.0 20.0 36.0 90-91 30.21619106433355 34.0 30.0 35.0 19.0 36.0 92-93 29.83899596509839 34.0 30.0 35.0 17.5 35.0 94-95 29.450598198105347 34.0 29.5 35.0 9.0 35.0 96-97 28.978209351836384 34.0 29.0 35.0 2.0 35.0 98-99 28.15830087893088 34.0 29.0 35.0 2.0 35.0 100-101 27.108232488498395 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34443871305841256 1101 2 0.22480253345850443 1101 3 0.3198223955293962 1101 4 0.27676931771077307 1101 5 0.2440534068726592 1101 6 0.14642007707064408 1101 7 0.23992327840821304 1101 8 0.22787348330491142 1101 9 0.24394977475029123 1101 10-11 0.28849800511879664 1101 12-13 0.3177407426594314 1101 14-15 0.2087159765382509 1101 16-17 0.30191702349765137 1101 18-19 0.38605526748048646 1101 20-21 0.3159463266600895 1101 22-23 0.43672368465834666 1101 24-25 0.32794868913826036 1101 26-27 0.3276658662854146 1101 28-29 0.29217794838992717 1101 30-31 0.32892938459325904 1101 32-33 0.3752315001913189 1101 34-35 0.41584666621321276 1101 36-37 0.3472582126337258 1101 38-39 0.2825038771048938 1101 40-41 0.3594527823835634 1101 42-43 0.32617070914196944 1101 44-45 0.2722261326083668 1101 46-47 0.3134587073171389 1101 48-49 0.21271205989025788 1101 50-51 0.24543477597561747 1101 52-53 0.2972230008845287 1101 54-55 0.3177553416298977 1101 56-57 0.41725395549109834 1101 58-59 0.40450072375560353 1101 60-61 0.3688564889068644 1101 62-63 0.3564448536251916 1101 64-65 0.27252884834346247 1101 66-67 0.37476272433734437 1101 68-69 0.3167828555760934 1101 70-71 0.35307163201331804 1101 72-73 0.37269880338291017 1101 74-75 0.34750443671559594 1101 76-77 0.29135831703815995 1101 78-79 0.35896883958407244 1101 80-81 0.33004363734454856 1101 82-83 0.5107615947665032 1101 84-85 0.4584509010763256 1101 86-87 0.44065818033003623 1101 88-89 0.5490879226198011 1101 90-91 0.5186169324581229 1101 92-93 0.5464956046251785 1101 94-95 0.46017001582874073 1101 96-97 0.43327873160401253 1101 98-99 0.8648814019086544 1101 100-101 1.0195092537305506 1102 1 0.27173255982170375 1102 2 0.3134482872979554 1102 3 0.29049251641727025 1102 4 0.25122179803187805 1102 5 0.24972076177658664 1102 6 0.1924360645724903 1102 7 0.2756460417563886 1102 8 0.16062122186346528 1102 9 0.25661974854908465 1102 10-11 0.29070406158005824 1102 12-13 0.31433474257077165 1102 14-15 0.34874816233944017 1102 16-17 0.22485886714238035 1102 18-19 0.30169670213064137 1102 20-21 0.3190073018053212 1102 22-23 0.435612359675396 1102 24-25 0.37487104185250786 1102 26-27 0.4401275242940059 1102 28-29 0.3940306525007671 1102 30-31 0.44218425210792844 1102 32-33 0.4535656104205401 1102 34-35 0.40791011956169854 1102 36-37 0.4220687288052716 1102 38-39 0.385889794388099 1102 40-41 0.31943763258046687 1102 42-43 0.3461249515866456 1102 44-45 0.4039624057616038 1102 46-47 0.39407808300205716 1102 48-49 0.3107684633583503 1102 50-51 0.3466198480909881 1102 52-53 0.4114567785479366 1102 54-55 0.4039617969750182 1102 56-57 0.4640013633524305 1102 58-59 0.5345995978523206 1102 60-61 0.54939785779343 1102 62-63 0.38177821713338034 1102 64-65 0.35688709381852135 1102 66-67 0.4030101738223877 1102 68-69 0.36806755402706415 1102 70-71 0.3979834250743792 1102 72-73 0.5020002696169641 1102 74-75 0.38746139596346296 1102 76-77 0.29207445004838917 1102 78-79 0.4169359222825477 1102 80-81 0.5405250598298714 1102 82-83 0.5702388063401393 1102 84-85 0.49439564419941107 1102 86-87 0.5518596649542289 1102 88-89 0.42481636747734797 1102 90-91 0.519509876257537 1102 92-93 0.5122301108433973 1102 94-95 0.4568896332350363 1102 96-97 0.6854736657523262 1102 98-99 1.0022883086823988 1102 100-101 1.1175174494024382 1103 1 0.21971463117117906 1103 2 0.2747550693863481 1103 3 0.2656899459851978 1103 4 0.228620753967391 1103 5 0.21084200063363312 1103 6 0.09431708955633411 1103 7 0.25935867608834684 1103 8 0.0601115312769096 1103 9 0.24279086058139399 1103 10-11 0.21129894870022525 1103 12-13 0.2219647899966688 1103 14-15 0.2953269146441144 1103 16-17 0.28539869479961055 1103 18-19 0.22452676614186373 1103 20-21 0.3666913401768923 1103 22-23 0.33672450734103165 1103 24-25 0.3029705336339745 1103 26-27 0.15188964936699278 1103 28-29 0.2717464903181579 1103 30-31 0.25025525544673854 1103 32-33 0.2093303246285032 1103 34-35 0.20944775124264225 1103 36-37 0.28672452608816457 1103 38-39 0.29720620500041406 1103 40-41 0.20735975698676867 1103 42-43 0.23661880508073097 1103 44-45 0.17992353912524095 1103 46-47 0.22627681051019977 1103 48-49 0.20562226686181617 1103 50-51 0.19930874734514248 1103 52-53 0.15470167931317036 1103 54-55 0.3256510476171357 1103 56-57 0.2433072059072714 1103 58-59 0.21742595287740585 1103 60-61 0.27944900394212624 1103 62-63 0.3377510411721474 1103 64-65 0.14758705826304208 1103 66-67 0.2435420935639172 1103 68-69 0.3699505360522579 1103 70-71 0.2488960446038604 1103 72-73 0.23022734136831247 1103 74-75 0.24244279156159365 1103 76-77 0.20095715336596598 1103 78-79 0.34372223675384106 1103 80-81 0.32360151995840525 1103 82-83 0.5756935041073312 1103 84-85 0.33839869527128386 1103 86-87 0.2629826392766077 1103 88-89 0.3556580481854894 1103 90-91 0.43079120186581576 1103 92-93 0.3645451229823067 1103 94-95 0.4980186546615073 1103 96-97 0.4887076987902752 1103 98-99 1.044309355022758 1103 100-101 0.8466674898628987 1104 1 0.26842829243943456 1104 2 0.22013922701293254 1104 3 0.1441909147983047 1104 4 0.20148895520598842 1104 5 0.23107233537137972 1104 6 0.15308964312956874 1104 7 0.20749843434172277 1104 8 0.15629017156071967 1104 9 0.33829865604206333 1104 10-11 0.19237735549342005 1104 12-13 0.25255638702982 1104 14-15 0.34322616876170287 1104 16-17 0.3601276113331551 1104 18-19 0.176091309296865 1104 20-21 0.24947411204671965 1104 22-23 0.3325586672978105 1104 24-25 0.2441855593263682 1104 26-27 0.3728711819421022 1104 28-29 0.28402346626945274 1104 30-31 0.2956963056275512 1104 32-33 0.26699396988999524 1104 34-35 0.27005519399533995 1104 36-37 0.2302532379486948 1104 38-39 0.2767664409100874 1104 40-41 0.2796698304193015 1104 42-43 0.22346320298532163 1104 44-45 0.23709159416465297 1104 46-47 0.3107676649152893 1104 48-49 0.30822757535100465 1104 50-51 0.2684644298811776 1104 52-53 0.26349025217079003 1104 54-55 0.3106522994953167 1104 56-57 0.2446139155612883 1104 58-59 0.23321360329627083 1104 60-61 0.21133700104425657 1104 62-63 0.19682888112591712 1104 64-65 0.2763530242993255 1104 66-67 0.2221612491901297 1104 68-69 0.23332796528734434 1104 70-71 0.24238201820690364 1104 72-73 0.22188609435411166 1104 74-75 0.22521358078139286 1104 76-77 0.11721248177548205 1104 78-79 0.2523700259903734 1104 80-81 0.3777351232518278 1104 82-83 0.40545582415532166 1104 84-85 0.32100703446973355 1104 86-87 0.4433119969318824 1104 88-89 0.49865074089681016 1104 90-91 0.49109995453661526 1104 92-93 0.45564605273498415 1104 94-95 0.5289003177732212 1104 96-97 0.49949216308425193 1104 98-99 0.9594008602042301 1104 100-101 0.9994137300874151 1105 1 0.20284348123357887 1105 2 0.14676786564081468 1105 3 0.10179611146948986 1105 4 0.13143813572633434 1105 5 0.17303436492506563 1105 6 0.022545073141372995 1105 7 0.15071907395912376 1105 8 0.0572210918263778 1105 9 0.1571774667892072 1105 10-11 0.016423397228351178 1105 12-13 0.08628211460959534 1105 14-15 0.08368282356521206 1105 16-17 0.06195059517457935 1105 18-19 0.178644101480522 1105 20-21 0.13545578962294513 1105 22-23 0.25218315874153774 1105 24-25 0.20842416078685488 1105 26-27 0.13720792955290761 1105 28-29 0.1484825593970811 1105 30-31 0.0810460820557708 1105 32-33 0.2198157557514051 1105 34-35 0.16202337768080355 1105 36-37 0.141454875616013 1105 38-39 0.2221747356591024 1105 40-41 0.13641082417448303 1105 42-43 0.20280158542028204 1105 44-45 0.21151867973187422 1105 46-47 0.18287490808336315 1105 48-49 0.17575006122009285 1105 50-51 0.17444770400808096 1105 52-53 0.18866106425981144 1105 54-55 0.19779285739459596 1105 56-57 0.2038572858157579 1105 58-59 0.1416920082878761 1105 60-61 0.012497051847127238 1105 62-63 0.12049213140905124 1105 64-65 0.19076097879916176 1105 66-67 0.21448160959746332 1105 68-69 0.2505824173531437 1105 70-71 0.24036074131385732 1105 72-73 0.29376191057647105 1105 74-75 0.2971727764778862 1105 76-77 0.20249999596754975 1105 78-79 0.3132479652363145 1105 80-81 0.41003765267491943 1105 82-83 0.4511839596024352 1105 84-85 0.22686332571167966 1105 86-87 0.2209726767765332 1105 88-89 0.32949062820835806 1105 90-91 0.560490501895508 1105 92-93 0.645492743774362 1105 94-95 0.6134058380436045 1105 96-97 0.6479860451401436 1105 98-99 1.117681642436299 1105 100-101 1.2186843599663462 1106 1 0.09223422300370387 1106 2 0.1062220538839469 1106 3 0.07359764967045379 1106 4 0.110966004286837 1106 5 0.11734953182181584 1106 6 -0.018009656056626966 1106 7 0.04805221392647496 1106 8 0.037384340075234945 1106 9 0.12364616706311438 1106 10-11 -0.0072596974381440305 1106 12-13 0.051996886860749214 1106 14-15 0.00926446386350932 1106 16-17 0.028503118594684906 1106 18-19 0.0792102341165446 1106 20-21 0.0910632667260316 1106 22-23 0.22696559579159015 1106 24-25 0.12589625289835737 1106 26-27 0.1134181938174379 1106 28-29 0.12538973289331778 1106 30-31 0.183857374216295 1106 32-33 0.123256206204168 1106 34-35 0.10054969640765421 1106 36-37 0.01932845398157923 1106 38-39 0.12275502893547952 1106 40-41 0.11360874707670376 1106 42-43 0.17370988659659758 1106 44-45 0.026476641001181633 1106 46-47 0.03935769046972837 1106 48-49 0.08483884210335191 1106 50-51 0.10654933459581173 1106 52-53 0.1437352239496832 1106 54-55 0.1881616087148572 1106 56-57 0.15903010170248422 1106 58-59 0.06008964913959858 1106 60-61 0.18888236852303208 1106 62-63 0.18446194774394087 1106 64-65 0.1358597198077689 1106 66-67 0.10674969102817755 1106 68-69 0.20837870199655129 1106 70-71 0.21463319118605995 1106 72-73 0.29113792950719386 1106 74-75 0.34968478562548455 1106 76-77 0.18808672144866279 1106 78-79 0.3399871806499064 1106 80-81 0.40403008705061794 1106 82-83 0.6236449057530216 1106 84-85 0.5821985639032974 1106 86-87 0.5702287696682191 1106 88-89 0.5813624643428703 1106 90-91 0.5107197853969616 1106 92-93 0.586403253258112 1106 94-95 0.6849687394360231 1106 96-97 0.7257086890103075 1106 98-99 1.1154518498882418 1106 100-101 1.1668865986299046 1107 1 0.16651816596361613 1107 2 0.061310469643025556 1107 3 0.02478106103514932 1107 4 0.01516783279623013 1107 5 -0.0028861803174180523 1107 6 0.008817583857307909 1107 7 0.02661931213358315 1107 8 0.04803596468435245 1107 9 0.07772646309086895 1107 10-11 0.020326413801257104 1107 12-13 0.007509891409490876 1107 14-15 -0.0012945742293410945 1107 16-17 0.01404118731591808 1107 18-19 0.008715776671714082 1107 20-21 0.0336249587927 1107 22-23 0.14400717297958465 1107 24-25 0.15161649618568873 1107 26-27 0.0960694542765026 1107 28-29 0.06337112085695651 1107 30-31 -0.0010780942792720793 1107 32-33 0.04844756700391173 1107 34-35 0.16016005649206022 1107 36-37 0.14092093404104133 1107 38-39 0.12361583978452728 1107 40-41 0.11165618197572513 1107 42-43 0.03463354572328825 1107 44-45 0.09122693268999171 1107 46-47 0.08550504719033825 1107 48-49 0.051489300822161965 1107 50-51 0.1369771206278756 1107 52-53 0.1089125820511967 1107 54-55 0.08962201927877089 1107 56-57 0.1380596017401743 1107 58-59 0.0750146112868535 1107 60-61 0.06266224139542231 1107 62-63 0.147947850446279 1107 64-65 0.2562582977229084 1107 66-67 0.19917333758197486 1107 68-69 0.21738463764538096 1107 70-71 0.21615423235449072 1107 72-73 0.19015173646454997 1107 74-75 0.2548884985279969 1107 76-77 0.16852529390816073 1107 78-79 0.18344574001818614 1107 80-81 0.2639390687258434 1107 82-83 0.37599971307218993 1107 84-85 0.4143808207790052 1107 86-87 0.4758382002233219 1107 88-89 0.40844803203849267 1107 90-91 0.559544122992925 1107 92-93 0.5462902060259971 1107 94-95 0.48370751608279505 1107 96-97 0.5627111886339478 1107 98-99 0.9430197441899928 1107 100-101 1.0219448410134007 1108 1 0.12106973862072579 1108 2 0.03176266826602614 1108 3 -0.017534911662426822 1108 4 3.33031321510191E-4 1108 5 0.022138341819271545 1108 6 0.004279321370098899 1108 7 -0.06331077048820788 1108 8 0.09182566352970412 1108 9 0.048634342061028235 1108 10-11 0.00940419887300692 1108 12-13 -0.10921003683392172 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2316 92-93 -1.2630524121906817 2316 94-95 -1.4058702360218547 2316 96-97 -1.4968307654145825 2316 98-99 -1.168042988023526 2316 100-101 -1.508129663136554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 178.0 3 93.0 4 315.0 5 872.0 6 1722.0 7 2952.0 8 4919.0 9 7754.0 10 10566.0 11 13377.0 12 16275.0 13 19722.0 14 22706.0 15 25866.0 16 30539.0 17 35347.0 18 40722.0 19 46982.0 20 50289.0 21 53896.0 22 59656.0 23 71546.0 24 87775.0 25 111174.0 26 141607.0 27 178719.0 28 223733.0 29 277881.0 30 339366.0 31 410790.0 32 499999.0 33 618131.0 34 798916.0 35 1114914.0 36 1414307.0 37 1972053.0 38 1762696.0 39 359378.0 40 536.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.292382431066756 31.64428628220466 27.930531118905762 22.132800167822822 2 22.72075065737654 29.350563788173346 28.11619290211575 19.812492652334367 3 20.928248088406374 28.527874584571183 28.690883094980368 21.852994232042075 4 21.521136916655493 29.04438831920167 28.25081888305349 21.183655881089354 5 22.206876501921972 28.89087198076801 28.089806910819693 20.812444606490327 6 21.907984864052978 28.7594352630182 27.919799133046467 21.412780739882358 7 22.122101377803684 29.018923467582198 27.868506521091867 20.990468633522248 8 21.338738444713552 28.82969567896771 28.228777840668716 21.60278803565002 9 21.144579620631397 28.656482508031072 28.642121930080144 21.556815941257387 10-11 21.226534976492957 29.164807331641214 28.063299090785172 21.545358601080654 12-13 21.21834985813522 29.230161348965378 27.89217279326917 21.659315999630227 14-15 21.4060668422626 29.241118779003365 27.709294994426166 21.643519384307872 16-17 21.595115044273943 29.041371905714175 27.62819088485636 21.73532216515553 18-19 21.550740083698518 29.09230132222894 27.611575202443493 21.74538339162905 20-21 21.690798409237892 28.99641207657475 27.65002420977905 21.662765304408303 22-23 21.720765750363462 28.987258042135807 27.631201946952878 21.66077426054786 24-25 21.590782423303303 28.99759416763658 27.591482996959165 21.820140412100955 26-27 21.775832927450722 28.854485681625125 27.740820449908178 21.628860941015976 28-29 21.75940555347678 28.94036772678704 27.626421177452933 21.67380554228325 30-31 21.74158223799714 28.961824283576153 27.60307698332028 21.693516495106426 32-33 21.75764720082669 28.98548007646104 27.530135383253423 21.72673733945885 34-35 21.765793378095903 29.028798450190497 27.495118173020845 21.71028999869276 36-37 21.7684242411789 29.01738534769426 27.478934109945513 21.735256301181334 38-39 21.77749717207355 29.01926074537016 27.48187631263603 21.721365769920265 40-41 21.75574503627462 29.118870634494638 27.427531105593705 21.697853223637036 42-43 21.779320935350135 29.104398079119452 27.399768510972176 21.716512474558233 44-45 21.790119429244566 29.128307950792053 27.392688754988647 21.688883864974734 46-47 21.813218443619103 29.169163054427827 27.32593531302463 21.691683188928444 48-49 21.805999639893965 29.163850692594544 27.310126612967245 21.720023054544242 50-51 21.829157294264714 29.140956153552384 27.305638587866298 21.724247964316604 52-53 21.755638343725316 29.237446387722493 27.265408661895833 21.74150660665635 54-55 21.80536085079242 29.231651161143326 27.19899346552395 21.763994522540308 56-57 21.800263349694333 29.24693402072443 27.18543028621225 21.767372343368994 58-59 21.782974501546757 29.3388517906157 27.151030550287135 21.72714315755041 60-61 21.765585144106836 29.324683812559694 27.140156341708742 21.769574701624734 62-63 21.758140273691247 29.381424162330788 27.134687382829743 21.72574818114822 64-65 21.749755672631572 29.446650746811738 27.101957643458203 21.701635937098487 66-67 21.7544979534639 29.479202611668292 27.065836443622143 21.70046299124566 68-69 21.706304464482336 29.52299391239488 27.090657814419867 21.68004380870292 70-71 21.743091149400325 29.57816504127161 27.016294801144667 21.662449008183398 72-73 21.71091217399211 29.5521735229413 27.026708649691795 21.710205653374796 74-75 21.768555603676432 29.57538132535603 27.00571889332125 21.650344177646282 76-77 21.721072400988813 29.684066019694534 26.964806719902995 21.63005485941366 78-79 21.71718533679334 29.67016216774323 26.939500701369003 21.673151794094426 80-81 21.679349778258146 29.728133678547803 26.92061949653981 21.671897046654237 82-83 21.743464191118616 29.788747548032703 26.863509000705577 21.604279260143112 84-85 21.680431411600768 29.830068837423383 26.858549941555744 21.630949809420105 86-87 21.643130156793394 29.877800949033308 26.856750385179414 21.622318508993892 88-89 21.642165479909913 29.961149773864683 26.824798390801984 21.57188635542342 90-91 21.614672390391448 29.929710041801243 26.82434954013831 21.631268027669 92-93 21.60699840849551 30.006439714968035 26.765904586013505 21.620657290522942 94-95 21.591230852062996 30.071484707970587 26.76277383385404 21.57451060611238 96-97 21.58168180201609 30.10787220718379 26.73249519333022 21.5779507974699 98-99 21.605760517396753 30.097397168796846 26.655812678370616 21.64102963543578 100-101 21.645193733850395 30.14710740970062 26.6003321221644 21.607366734284582 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 207300.0 1 118430.5 2 21597.0 3 12517.5 4 10768.0 5 9318.5 6 8480.0 7 8415.0 8 8098.5 9 7428.5 10 6949.0 11 7615.5 12 12929.5 13 13037.0 14 8300.0 15 8241.5 16 8981.0 17 10322.0 18 12387.0 19 14609.5 20 17882.5 21 23060.0 22 29469.5 23 37049.0 24 45729.0 25 55907.0 26 69887.0 27 85941.0 28 98874.0 29 110828.0 30 127798.5 31 149739.0 32 171889.0 33 194297.0 34 217995.5 35 241573.0 36 266548.5 37 289350.0 38 306204.0 39 314152.5 40 323048.5 41 338141.5 42 351463.5 43 368496.5 44 383850.0 45 395377.0 46 403498.0 47 406625.5 48 415580.5 49 422635.0 50 418461.5 51 405150.5 52 386816.5 53 370869.0 54 349407.5 55 320887.0 56 290793.0 57 260224.5 58 232030.5 59 197955.5 60 158984.0 61 121142.0 62 86140.5 63 59056.5 64 39219.5 65 23982.5 66 13797.0 67 8239.5 68 4713.5 69 2417.0 70 1220.0 71 637.5 72 365.5 73 226.0 74 148.0 75 100.0 76 74.0 77 61.0 78 47.5 79 40.0 80 27.5 81 16.0 82 13.5 83 10.0 84 8.0 85 6.5 86 5.0 87 3.5 88 3.5 89 2.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15576820265547522 2 0.0 3 0.0 4 9.696840741581134E-4 5 5.541051852332076E-4 6 6.464560494387422E-5 7 1.9393681483162266E-4 8 0.0 9 1.2929120988774845E-4 10-11 2.308771605138365E-4 12-13 0.0 14-15 0.0 16-17 2.770525926166038E-5 18-19 4.617543210276731E-6 20-21 0.0 22-23 6.141332469668052E-4 24-25 0.0 26-27 1.385262963083019E-4 28-29 0.005388672926392944 30-31 0.003610918790436403 32-33 8.773332099525788E-5 34-35 9.188910988450693E-4 36-37 3.832560864529686E-4 38-39 9.743016173683901E-4 40-41 0.002890582049633233 42-43 0.0010158595062608807 44-45 5.402525556023774E-4 46-47 0.008847212790890216 48-49 0.008334665494549498 50-51 0.007609711210536052 52-53 0.014540643569161424 54-55 0.005744223753584253 56-57 7.480420000648303E-4 58-59 0.0011266805433075221 60-61 4.617543210276731E-6 62-63 8.311577778498115E-5 64-65 6.603086790695725E-4 66-67 8.72715666742302E-4 68-69 0.002618147000226906 70-71 0.00169463835817156 72-73 0.005167030852299661 74-75 0.0019624558643676106 76-77 0.0012605892964055474 78-79 0.0014776138272885537 80-81 2.262596173035598E-4 82-83 0.0021148347903067423 84-85 1.8470172841106923E-5 86-87 0.0018285471112695854 88-89 1.662315555699623E-4 90-91 0.0011266805433075221 92-93 0.001385262963083019 94-95 7.295718272237234E-4 96-97 7.942174321675977E-4 98-99 0.5246083192059598 100-101 2.262596173035598E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0828269E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.43778040149022 #Duplication Level Percentage of deduplicated Percentage of total 1 90.17930629817124 59.913129485966245 2 5.5711251978301455 7.402663849652959 3 1.3609904038867016 2.712635447458805 4 0.707660726345194 1.8806163174272423 5 0.3980698595301871 1.3223438955959312 6 0.2455629927886947 0.9788796113776813 7 0.16123979411627684 0.7498689823435086 8 0.1219471938429521 0.648152070809281 9 0.0912911425929427 0.5458662795771951 >10 1.0317861125011265 15.135944970775341 >50 0.11805643724074379 5.21979484584724 >100 0.01240626403805452 1.013626322452408 >500 2.2303084630477023E-4 0.08972101842874616 >1k 1.1151542315191435E-4 0.16532820200076018 >5k 1.1151542315191435E-4 0.4539764991080699 >10k+ 1.1151542315191435E-4 1.76745220117864 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33440 0.3088212899033077 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 14272 0.13180315339413898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.61754321027673E-5 0.0 0.0 0.0 0.0 2 5.541051852332076E-5 0.0 0.0 0.0 9.23508642055346E-6 3 1.0158595062608807E-4 0.0 0.0 0.0 9.23508642055346E-6 4 1.7546664199051575E-4 0.0 0.0 0.0 9.23508642055346E-6 5 1.847017284110692E-4 0.0 0.0 0.0 1.847017284110692E-5 6 2.4011224693438996E-4 0.0 0.0 0.0 3.694034568221384E-5 7 2.6781750619605035E-4 0.0 0.0 0.0 4.61754321027673E-5 8 3.047578518782642E-4 0.0 0.0 0.0 4.61754321027673E-5 9 3.509332839810315E-4 0.0 0.0 0.0 5.541051852332076E-5 10-11 4.432841481865661E-4 9.23508642055346E-6 0.0 0.0 6.464560494387422E-5 12-13 5.079297531304403E-4 9.23508642055346E-6 0.0 0.0 6.926314815415095E-5 14-15 5.356350123921007E-4 9.23508642055346E-6 0.0 0.0 7.388069136442768E-5 16-17 5.679578148640378E-4 2.308771605138365E-5 0.0 0.0 8.773332099525788E-5 18-19 6.464560494387423E-4 3.232280247193711E-5 0.0 0.0 9.23508642055346E-5 20-21 7.157191975928932E-4 3.694034568221384E-5 0.0 0.0 1.1082103704664153E-4 22-23 7.665121729059372E-4 3.694034568221384E-5 0.0 0.0 1.1082103704664153E-4 24-25 8.773332099525788E-4 3.694034568221384E-5 9.23508642055346E-6 9.23508642055346E-6 1.1082103704664153E-4 26-27 9.650665309478366E-4 3.694034568221384E-5 9.23508642055346E-6 9.23508642055346E-6 1.1082103704664153E-4 28-29 9.881542469992203E-4 4.61754321027673E-5 9.23508642055346E-6 1.3852629630830189E-5 1.1543858025691825E-4 30-31 0.001043564765522541 4.61754321027673E-5 9.23508642055346E-6 1.847017284110692E-5 1.3390875309802518E-4 32-33 0.001094357740835585 6.464560494387422E-5 9.23508642055346E-6 1.847017284110692E-5 1.4776138272885536E-4 34-35 0.0011913261482513963 6.464560494387422E-5 9.23508642055346E-6 1.847017284110692E-5 1.6161401235968557E-4 36-37 0.0013252349013494215 7.388069136442768E-5 9.23508642055346E-6 1.847017284110692E-5 1.7546664199051575E-4 38-39 0.0014637611976577236 7.388069136442768E-5 9.23508642055346E-6 1.847017284110692E-5 1.7546664199051575E-4 40-41 0.0016253752100174091 9.23508642055346E-5 9.23508642055346E-6 1.847017284110692E-5 1.8931927162134593E-4 42-43 0.001763901506325711 9.23508642055346E-5 9.23508642055346E-6 1.847017284110692E-5 2.0778944446245287E-4 44-45 0.0019208979754751197 9.23508642055346E-5 9.23508642055346E-6 1.847017284110692E-5 2.4011224693439E-4 46-47 0.0020594242717834215 9.23508642055346E-5 9.23508642055346E-6 1.847017284110692E-5 2.4934733335494344E-4 48-49 0.002142540049568403 9.696840741581133E-5 9.23508642055346E-6 1.847017284110692E-5 2.5396487656522017E-4 50-51 0.002322624234769195 1.0158595062608807E-4 9.23508642055346E-6 1.847017284110692E-5 2.770525926166038E-4 52-53 0.0025119435063905415 1.2005612346719498E-4 9.23508642055346E-6 1.847017284110692E-5 2.770525926166038E-4 54-55 0.002664322432329673 1.2005612346719498E-4 9.23508642055346E-6 1.847017284110692E-5 2.8628767903715726E-4 56-57 0.0028028487286379756 1.2005612346719498E-4 9.23508642055346E-6 1.847017284110692E-5 2.90905222247434E-4 58-59 0.0029829329138387674 1.2929120988774845E-4 9.23508642055346E-6 1.847017284110692E-5 3.0475785187826423E-4 60-61 0.00316301709903956 1.385262963083019E-4 9.23508642055346E-6 1.847017284110692E-5 3.186104815090944E-4 62-63 0.0034262170620253337 1.385262963083019E-4 9.23508642055346E-6 1.847017284110692E-5 3.2322802471937114E-4 64-65 0.0036386240496980634 1.385262963083019E-4 9.23508642055346E-6 1.847017284110692E-5 3.370806543502013E-4 66-67 0.0038695012102119 1.4776138272885536E-4 9.23508642055346E-6 1.847017284110692E-5 3.6016837040158496E-4 68-69 0.0041511713460387805 1.5237892593913211E-4 9.23508642055346E-6 1.847017284110692E-5 3.7863854324269187E-4 70-71 0.004460546741127322 1.662315555699623E-4 9.23508642055346E-6 2.308771605138365E-5 3.9249117287352205E-4 72-73 0.004825332654739183 1.662315555699623E-4 9.23508642055346E-6 2.770525926166038E-5 4.0172625929407556E-4 74-75 0.005203971197981875 1.662315555699623E-4 9.23508642055346E-6 2.770525926166038E-5 4.10961345714629E-4 76-77 0.005619550086906781 1.7546664199051575E-4 9.23508642055346E-6 2.770525926166038E-5 4.1557888892490574E-4 78-79 0.00604436406225224 1.7546664199051575E-4 9.23508642055346E-6 2.770525926166038E-5 4.248139753454592E-4 80-81 0.006672349938849876 1.847017284110692E-4 9.23508642055346E-6 2.770525926166038E-5 4.3404906176601265E-4 82-83 0.007434244568545536 1.847017284110692E-4 9.23508642055346E-6 3.694034568221384E-5 4.386666049762894E-4 84-85 0.008459339161226969 1.847017284110692E-4 9.23508642055346E-6 4.61754321027673E-5 4.571367778173963E-4 86-87 0.009789191605786668 1.8931927162134593E-4 9.23508642055346E-6 6.00280617335975E-5 4.7098940744822647E-4 88-89 0.011751647470154277 1.985543580418994E-4 9.23508642055346E-6 7.388069136442768E-5 4.848420370790567E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 475 0.0 31.98465 3 CGCGTAG 305 0.0 24.906712 54-55 CGTACGC 315 0.0 24.115076 20-21 TCGCGTA 360 0.0 21.762499 52-53 CAACGCA 8330 0.0 20.261322 12-13 ACTCGCG 365 0.0 20.164444 50-51 ATCAACG 8700 0.0 19.42701 10-11 CTCGCGT 405 0.0 18.75825 52-53 ACGTACG 420 0.0 18.651505 20-21 GGTATCA 10445 0.0 17.99973 8 TCAACGC 8170 0.0 17.868917 10-11 AGTGGTA 10595 0.0 17.744896 5 GTATCAA 10855 0.0 17.538553 9 AACGCAG 8670 0.0 16.865717 12-13 ACGCAGA 9985 0.0 16.784168 14-15 TGGTATC 11535 0.0 16.38116 7 GTGGTAT 12025 0.0 15.8321 6 CGTAGCG 495 0.0 15.345993 56-57 CGCTAGT 465 0.0 15.315302 90-91 AAGCAGT 13875 0.0 14.564495 1 >>END_MODULE