FastQCFastQC Report
Sat 18 Jun 2016
SRR578554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9619357
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130966213.614860120068318No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT321280.3339932180498135No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCCGCAGCGGCTTCTGA149830.1557588516571326No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG128230.13330412833207042No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124560.12948890450785847No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA104540.10867670261120363No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT103100.1071797210561995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAAACTCATGC100820.10480950026077628No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC98560.10246007087583921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2937750.091.735595
GGTATCA2956800.091.40128
GTATCAA2958350.091.372569
TGGTATC2956850.091.297
GTGGTAT2960500.091.217246
AAGCAGT2966000.091.127171
AGCAGTG2983100.090.620362
GCAGTGG2981000.090.504753
CAGTGGT2984550.090.302254
TCAACGC2949450.046.3671212-13
AACGCAG2954800.046.2699514-15
CAACGCA2947800.046.2078712-13
ATCAACG2949250.046.18378410-11
CGCAGAG2961850.046.1464916-17
ACGCAGA2958750.046.04774514-15
TATCAAC2972200.045.985310-11
CAGAGTA2949400.045.77641318-19
AGAGTAC2894700.045.6396218-19
GAGTACT1650400.045.5715820-21
GCAGAGT2990850.045.3927616-17
Produced by FastQC (version 0.11.5)