FastQCFastQC Report
Sat 18 Jun 2016
SRR578554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578554_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9619357
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330351.3829926470137244No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290880.30239027411083713No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132290.13752478466076268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG2900.027.7968243
CGCGTAG1700.023.70424854-55
CAACGCA75350.022.30432912-13
ATCAACG78950.021.25726310-11
AGTGGTA97850.019.2378225
GGTATCA96750.019.2114018
TCGCGTA2150.018.74289552-53
GTATCAA99600.018.7083119
ACGCAGA92100.018.27363614-15
ACGTACG2450.017.41817520-21
CGTACGC2600.017.32512920-21
TGGTATC108750.017.3095267
TCAACGC75200.016.8640610-11
GTGGTAT113050.016.7350226
AACGCAG79750.015.842463512-13
AAGCAGT125450.015.6886261
AGAGTAC110950.014.91371818-19
TATCAAC107300.014.3610119
GCAGTGG149900.013.0328633
CGCAGAG99500.012.74550114-15
Produced by FastQC (version 0.11.5)