##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578554_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9619357 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.967934239263602 31.0 31.0 34.0 30.0 34.0 2 32.239242498225195 34.0 31.0 34.0 30.0 34.0 3 32.30697696322114 34.0 31.0 34.0 30.0 34.0 4 35.887332074274816 37.0 35.0 37.0 35.0 37.0 5 35.768628817913715 37.0 35.0 37.0 33.0 37.0 6 35.741083109817005 37.0 35.0 37.0 33.0 37.0 7 35.73586716866834 37.0 35.0 37.0 33.0 37.0 8 35.65311392435066 37.0 35.0 37.0 33.0 37.0 9 37.28678382557171 39.0 37.0 39.0 33.0 39.0 10-11 37.28079111732728 39.0 37.0 39.0 33.0 39.0 12-13 37.274443395748804 39.0 37.0 39.0 33.0 39.0 14-15 38.58078726052064 40.0 38.0 41.0 34.0 41.0 16-17 38.5310392888007 40.0 38.0 41.0 34.0 41.0 18-19 38.46194771646379 40.0 38.0 41.0 33.5 41.0 20-21 38.3934095594955 40.0 38.0 41.0 34.0 41.0 22-23 38.26990281159125 40.0 38.0 41.0 33.0 41.0 24-25 38.18633397221872 40.0 37.5 41.0 33.0 41.0 26-27 38.018574838214235 40.0 37.0 41.0 33.0 41.0 28-29 37.922363417845915 40.0 37.0 41.0 33.0 41.0 30-31 37.82540776893923 40.0 37.0 41.0 33.0 41.0 32-33 37.69199568120821 40.0 37.0 41.0 32.0 41.0 34-35 37.561145043270564 40.0 37.0 41.0 32.0 41.0 36-37 37.437604249431644 40.0 36.0 41.0 31.0 41.0 38-39 37.3103592579005 40.0 36.0 41.0 31.0 41.0 40-41 37.165438344787496 39.0 36.0 41.0 31.0 41.0 42-43 37.00785109649221 39.0 36.0 41.0 31.0 41.0 44-45 36.85549460322556 39.0 35.0 41.0 30.0 41.0 46-47 36.94201093690566 39.0 36.0 41.0 31.0 41.0 48-49 36.924310897287626 39.0 36.0 41.0 31.0 41.0 50-51 36.899941856820575 39.0 35.5 41.0 31.0 41.0 52-53 36.91698899417082 39.0 36.0 41.0 31.0 41.0 54-55 36.78997941338491 39.0 35.0 41.0 31.0 41.0 56-57 36.64133174389931 39.0 35.0 41.0 30.0 41.0 58-59 36.427730252656175 39.0 35.0 41.0 30.0 41.0 60-61 36.20354354246339 38.5 35.0 40.5 29.5 41.0 62-63 35.93221558364036 38.0 35.0 40.0 29.0 41.0 64-65 35.618497421397294 37.5 34.0 40.0 29.0 41.0 66-67 35.298619491926544 37.0 34.0 40.0 28.5 41.0 68-69 34.96672256783899 36.5 34.0 39.0 28.0 41.0 70-71 34.596331178892726 36.0 34.0 39.0 28.0 41.0 72-73 34.172107501572086 35.5 33.5 39.0 28.0 40.5 74-75 33.7835856388322 35.0 33.0 37.5 27.0 40.0 76-77 32.34465011538713 34.5 31.5 36.0 25.5 39.0 78-79 32.94572630998101 35.0 32.5 36.5 26.0 39.0 80-81 32.888997206362134 35.0 33.0 36.0 26.0 39.0 82-83 32.6099787646929 35.0 33.0 36.0 26.0 37.0 84-85 32.32520068649079 35.0 33.0 35.5 26.0 37.0 86-87 32.04304419723688 35.0 32.0 35.0 26.0 37.0 88-89 31.794971846870844 35.0 32.0 35.0 25.0 36.0 90-91 31.553677912151507 35.0 32.0 35.0 25.0 36.0 92-93 31.309357319829175 35.0 32.0 35.0 24.5 36.0 94-95 31.055816932462328 35.0 32.0 35.0 23.5 35.5 96-97 30.45110302071126 34.0 31.5 35.0 17.5 35.0 98-99 30.419824526732917 34.0 31.0 35.0 18.5 35.0 100-101 29.845006480162862 34.0 30.5 35.0 9.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4588412555059058 1101 2 0.4173293321111444 1101 3 0.37561996535379905 1101 4 0.29571572127231605 1101 5 0.2969045494501614 1101 6 0.3124158477580039 1101 7 0.32433844730488204 1101 8 0.3745144276394825 1101 9 0.4632095876764737 1101 10-11 0.4250429605634878 1101 12-13 0.43355321811224456 1101 14-15 0.5667260519645865 1101 16-17 0.5655873187227982 1101 18-19 0.5498167758843451 1101 20-21 0.6399474959001523 1101 22-23 0.6421246847856352 1101 24-25 0.6801569011357813 1101 26-27 0.6999195566476928 1101 28-29 0.7047620219477366 1101 30-31 0.6646097716799488 1101 32-33 0.6540625319507996 1101 34-35 0.6705025980846173 1101 36-37 0.6633968400156789 1101 38-39 0.6682486043715272 1101 40-41 0.7044778974334847 1101 42-43 0.732488115584971 1101 44-45 0.7402568044026623 1101 46-47 0.7370792019734225 1101 48-49 0.7295439456515354 1101 50-51 0.7022339271392255 1101 52-53 0.634529548392905 1101 54-55 0.705404227617727 1101 56-57 0.7010649340239254 1101 58-59 0.7642984616175426 1101 60-61 0.7081152807812785 1101 62-63 0.7676770731272953 1101 64-65 0.7361927008818299 1101 66-67 0.6917833387456369 1101 68-69 0.7155836762418559 1101 70-71 0.6869109145802668 1101 72-73 0.6244459920040413 1101 74-75 0.6872003802838691 1101 76-77 0.6650516567388891 1101 78-79 0.5827797186828221 1101 80-81 0.5011782822610584 1101 82-83 0.4946962655437872 1101 84-85 0.4246980187772209 1101 86-87 0.45482843866496125 1101 88-89 0.4860167196522944 1101 90-91 0.507385422993277 1101 92-93 0.514700093567491 1101 94-95 0.5632393233566653 1101 96-97 0.9044198207742902 1101 98-99 0.6791684240173872 1101 100-101 0.6502650240057442 1102 1 0.42677621914141994 1102 2 0.39727675231367954 1102 3 0.3939367077549889 1102 4 0.27674638513792615 1102 5 0.29437896337097413 1102 6 0.2733234804125786 1102 7 0.27463594021392623 1102 8 0.3343259059890755 1102 9 0.4599668372898691 1102 10-11 0.3947039751492767 1102 12-13 0.41913923310350043 1102 14-15 0.5093361562398613 1102 16-17 0.5456591858211084 1102 18-19 0.5716076429454588 1102 20-21 0.5990735945477397 1102 22-23 0.5790046201686394 1102 24-25 0.6521762292552111 1102 26-27 0.6983214067180867 1102 28-29 0.6922399828366181 1102 30-31 0.7429641303838466 1102 32-33 0.7780931933737776 1102 34-35 0.7849232227693719 1102 36-37 0.8025974420098763 1102 38-39 0.8160583861799964 1102 40-41 0.8040559043769804 1102 42-43 0.8304208926017083 1102 44-45 0.8979552523137144 1102 46-47 0.8490064983720131 1102 48-49 0.8707708715908282 1102 50-51 0.8830181221912881 1102 52-53 0.8487073922334005 1102 54-55 0.8169926623544299 1102 56-57 0.7660531086168376 1102 58-59 0.7998051101669006 1102 60-61 0.8762851810548682 1102 62-63 0.806799572539127 1102 64-65 0.7895749246927508 1102 66-67 0.8460733439107031 1102 68-69 0.8375766282758406 1102 70-71 0.7934169236230773 1102 72-73 0.8767406234564206 1102 74-75 0.7423859394614567 1102 76-77 0.7331339327955178 1102 78-79 0.7995370948847551 1102 80-81 0.8043714914961058 1102 82-83 0.8199690383793339 1102 84-85 0.7977671730952736 1102 86-87 0.7803253012892597 1102 88-89 0.6974740718689709 1102 90-91 0.7082953986645499 1102 92-93 0.7711495394702865 1102 94-95 0.7950774797172322 1102 96-97 1.2020938318877086 1102 98-99 0.9655796973109112 1102 100-101 0.9525095296955506 1103 1 0.2942654160042686 1103 2 0.2822227707388052 1103 3 0.26073606269559235 1103 4 0.2176123585629668 1103 5 0.20782358984249782 1103 6 0.26066340309426295 1103 7 0.27025743773866395 1103 8 0.09495207429467456 1103 9 0.16474615829817907 1103 10-11 0.3363657855161577 1103 12-13 0.32829985715317633 1103 14-15 0.4494648373126111 1103 16-17 0.42952700464477545 1103 18-19 0.36168930995936677 1103 20-21 0.37101362086222167 1103 22-23 0.4311693857875909 1103 24-25 0.2522844473405783 1103 26-27 0.2600793861877193 1103 28-29 0.46340107665429997 1103 30-31 0.27998843135484464 1103 32-33 0.5283573461440767 1103 34-35 0.4826588575376576 1103 36-37 0.5440545341561887 1103 38-39 0.579053746752372 1103 40-41 0.4615310996512605 1103 42-43 0.5516886989393939 1103 44-45 0.6865196868166734 1103 46-47 0.552409572029724 1103 48-49 0.5641757351608376 1103 50-51 0.49950364409156833 1103 52-53 0.5609952505509668 1103 54-55 0.6528138060323485 1103 56-57 0.5710987447756963 1103 58-59 0.33610414721982806 1103 60-61 0.30579522729401276 1103 62-63 0.13521902775850947 1103 64-65 0.38443132771238453 1103 66-67 0.37636242313057977 1103 68-69 0.32784842076014087 1103 70-71 0.3341116004593445 1103 72-73 0.5107330550521354 1103 74-75 0.4343539943563002 1103 76-77 0.28318774899529586 1103 78-79 0.4158716481513025 1103 80-81 0.4314646429818296 1103 82-83 0.43905702203310426 1103 84-85 0.4365156261296761 1103 86-87 0.30284013942063837 1103 88-89 0.3329723016949906 1103 90-91 0.3887078399702908 1103 92-93 0.3414642842611251 1103 94-95 0.368696339279186 1103 96-97 0.4993681822016782 1103 98-99 0.2433394267137885 1103 100-101 0.5041158042326508 1104 1 0.3561922594166482 1104 2 0.3378795408407598 1104 3 0.29354815404946066 1104 4 0.22775724412332465 1104 5 0.25847368208103205 1104 6 0.25306482248800677 1104 7 0.2517292234071675 1104 8 0.3182260484398114 1104 9 0.32943969551273256 1104 10-11 0.3821533413162186 1104 12-13 0.37709791832394757 1104 14-15 0.42398632301127037 1104 16-17 0.4674007081552176 1104 18-19 0.44659731775040257 1104 20-21 0.4128875298453849 1104 22-23 0.5476369577057554 1104 24-25 0.48502103484975123 1104 26-27 0.5539813733049783 1104 28-29 0.5317830873143166 1104 30-31 0.5682474129258779 1104 32-33 0.5997211717442426 1104 34-35 0.5884231582705226 1104 36-37 0.6330868275444956 1104 38-39 0.6676323027358819 1104 40-41 0.5905686184186081 1104 42-43 0.6099892816347392 1104 44-45 0.6171094171965237 1104 46-47 0.4412981333789645 1104 48-49 0.5970850278108273 1104 50-51 0.49599939011280725 1104 52-53 0.5847692018178421 1104 54-55 0.6572396530635558 1104 56-57 0.6879193883397576 1104 58-59 0.7084171604299314 1104 60-61 0.6073548969702998 1104 62-63 0.45841640636326986 1104 64-65 0.6370842456603398 1104 66-67 0.6717459206411078 1104 68-69 0.7669372748453398 1104 70-71 0.7055293299256178 1104 72-73 0.7956698925206211 1104 74-75 0.7207974623581777 1104 76-77 0.5879844091194926 1104 78-79 0.5725708065959765 1104 80-81 0.7171519376744655 1104 82-83 0.7722773560457554 1104 84-85 0.7933592225951855 1104 86-87 0.734317449013524 1104 88-89 0.6727541235306411 1104 90-91 0.6706694140651059 1104 92-93 0.6984344631179198 1104 94-95 0.7597547348881406 1104 96-97 1.1188343775528757 1104 98-99 0.8577648830182092 1104 100-101 0.9498442580799455 1105 1 0.13721115693205022 1105 2 0.1656364060930926 1105 3 0.1431197317024413 1105 4 0.06733620308019539 1105 5 0.12865319997619906 1105 6 0.053855951478304576 1105 7 0.09309469810801829 1105 8 0.16895294175092346 1105 9 0.17165877257569662 1105 10-11 0.14809394605568116 1105 12-13 0.173807624453012 1105 14-15 0.2015463972730842 1105 16-17 0.16124097797263914 1105 18-19 0.10315240790375668 1105 20-21 0.2020073002407159 1105 22-23 0.20548891528685687 1105 24-25 0.2589211083752829 1105 26-27 0.21008308195117564 1105 28-29 0.24943443511484276 1105 30-31 0.18578043064000127 1105 32-33 0.21185262782532988 1105 34-35 0.15840823034431395 1105 36-37 0.17088574627319275 1105 38-39 0.2101529650221039 1105 40-41 0.24720725906160368 1105 42-43 0.15382469840919555 1105 44-45 0.2919905372466971 1105 46-47 0.2567591363264583 1105 48-49 0.23494590892713774 1105 50-51 0.09187008241314487 1105 52-53 0.22179865545641775 1105 54-55 0.3085080001241849 1105 56-57 0.3070167652122038 1105 58-59 0.2906667235155709 1105 60-61 0.2874662747965786 1105 62-63 0.35364941652763804 1105 64-65 0.3220961291793074 1105 66-67 0.32202105034385653 1105 68-69 0.3033326010206068 1105 70-71 0.347128821011502 1105 72-73 0.37829498455495525 1105 74-75 0.3642746360865772 1105 76-77 0.41039007670001837 1105 78-79 0.36956548769486375 1105 80-81 0.3190894355254841 1105 82-83 0.2787810766053056 1105 84-85 0.3805246222061882 1105 86-87 0.28921684191071506 1105 88-89 0.2581132319173314 1105 90-91 0.298870534986694 1105 92-93 0.30568042415705676 1105 94-95 0.40144063327042545 1105 96-97 0.7551206876681889 1105 98-99 0.5335662490083415 1105 100-101 0.5285668213815775 1106 1 -0.025743969928868182 1106 2 -0.058348451279343294 1106 3 -0.02926263410261498 1106 4 -0.05057118925395798 1106 5 -0.02102751071696929 1106 6 -0.027992493817848185 1106 7 -0.032505636085033984 1106 8 -0.040564800928450495 1106 9 0.02324661076852408 1106 10-11 -0.06665288177195094 1106 12-13 -0.08639259947461397 1106 14-15 -0.1702524588053933 1106 16-17 -0.10495603882575466 1106 18-19 -0.13309957050008592 1106 20-21 -0.1434380654590015 1106 22-23 -0.10277619570177876 1106 24-25 -0.006439859114998114 1106 26-27 -0.06686007605367905 1106 28-29 -0.11056703599498263 1106 30-31 -0.21778498472668417 1106 32-33 -0.24083234776291107 1106 34-35 -0.31819911247838206 1106 36-37 -0.1924138838919447 1106 38-39 -0.2647031190964171 1106 40-41 -0.2626356375125809 1106 42-43 -0.2519376848174417 1106 44-45 -0.22309247298912993 1106 46-47 -0.27164096455769027 1106 48-49 -0.2773806288067533 1106 50-51 -0.2794006111198044 1106 52-53 -0.18319120035271652 1106 54-55 -0.20482687928158327 1106 56-57 -0.23294192634111965 1106 58-59 -0.16768813399525584 1106 60-61 -0.3032032940804896 1106 62-63 -0.22447885074277707 1106 64-65 -0.1841280399598233 1106 66-67 -0.19075892471790468 1106 68-69 -0.17090463684119328 1106 70-71 -0.18138874527266324 1106 72-73 -0.1641875904994876 1106 74-75 -0.13685777049465742 1106 76-77 -0.24988185157121023 1106 78-79 -0.13774447730425265 1106 80-81 -0.21353806817993615 1106 82-83 -0.20311120022673634 1106 84-85 -0.23394694120145232 1106 86-87 -0.324055229876663 1106 88-89 -0.34426242460948586 1106 90-91 -0.291560030753093 1106 92-93 -0.21098414998607495 1106 94-95 -0.18286934101610441 1106 96-97 0.08187753700738298 1106 98-99 -0.12292172944511748 1106 100-101 -0.1416130036445331 1107 1 -0.19095218368275013 1107 2 -0.26321326098350184 1107 3 -0.2044301631803762 1107 4 -0.18297228100902174 1107 5 -0.1609347540732955 1107 6 -0.15291534757641045 1107 7 -0.21916092950321087 1107 8 -0.21283115444735046 1107 9 -0.254130530075642 1107 10-11 -0.2708796460597611 1107 12-13 -0.24223486287768736 1107 14-15 -0.2989757539776434 1107 16-17 -0.30802652861709845 1107 18-19 -0.28778781894501293 1107 20-21 -0.3389947639990467 1107 22-23 -0.24475864857659246 1107 24-25 -0.19774926294784478 1107 26-27 -0.23629664174408305 1107 28-29 -0.19638587742107205 1107 30-31 -0.29429377033073933 1107 32-33 -0.32351241790819074 1107 34-35 -0.3103002985008274 1107 36-37 -0.3576801974232069 1107 38-39 -0.37237518595907915 1107 40-41 -0.3416535410867212 1107 42-43 -0.4268117084433527 1107 44-45 -0.35493284309669093 1107 46-47 -0.34333407352691125 1107 48-49 -0.4222944564425859 1107 50-51 -0.41212931747484305 1107 52-53 -0.4428608908542415 1107 54-55 -0.43996344067527815 1107 56-57 -0.4431055076221284 1107 58-59 -0.4192731170735158 1107 60-61 -0.507861464063609 1107 62-63 -0.5784868563229324 1107 64-65 -0.5638105553012664 1107 66-67 -0.4448645558179507 1107 68-69 -0.43383787486899195 1107 70-71 -0.4234162927127727 1107 72-73 -0.4139948804359648 1107 74-75 -0.4579050195845866 1107 76-77 -0.5070746450541535 1107 78-79 -0.30563358605976987 1107 80-81 -0.36187556436973267 1107 82-83 -0.36472918672343724 1107 84-85 -0.3736941721095377 1107 86-87 -0.3963010560164051 1107 88-89 -0.40944012601807955 1107 90-91 -0.41020647660526777 1107 92-93 -0.39046126254355684 1107 94-95 -0.40379363538942314 1107 96-97 -0.10805868859263867 1107 98-99 -0.4065895965676205 1107 100-101 -0.4922775841409468 1108 1 -0.3537856521356346 1108 2 -0.34061834366491084 1108 3 -0.3439206457294226 1108 4 -0.2027889873480575 1108 5 -0.2488588823461768 1108 6 -0.25396347722890056 1108 7 -0.24812484915519661 1108 8 -0.22475697178778375 1108 9 -0.34250854849801726 1108 10-11 -0.39132826075450566 1108 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-0.13153177881340383 2305 28-29 -0.17491395723259018 2305 30-31 -0.23071293740778032 2305 32-33 -0.20313160984816392 2305 34-35 -0.1853414580390833 2305 36-37 -0.13151027467926468 2305 38-39 -0.15851087433692612 2305 40-41 -0.17200103009776768 2305 42-43 -0.1587733572909258 2305 44-45 -0.16832838403958306 2305 46-47 -0.06650373534927922 2305 48-49 -0.13242554314406618 2305 50-51 -0.13419525458106563 2305 52-53 -0.17007868312998653 2305 54-55 -0.062420104828440515 2305 56-57 -0.07793548079455803 2305 58-59 -0.038267463551683534 2305 60-61 -0.03852287029616974 2305 62-63 -0.043007645845783316 2305 64-65 -0.013231568067439525 2305 66-67 -0.13277609732791973 2305 68-69 -0.12899180392081888 2305 70-71 -0.08324952950518849 2305 72-73 -0.06356246963557766 2305 74-75 -0.0809210702666121 2305 76-77 -0.16150800181388547 2305 78-79 -0.1299627084171604 2305 80-81 -0.08360526836690241 2305 82-83 -0.06231317089918065 2305 84-85 -0.029819939209168922 2305 86-87 -0.03815093215028753 2305 88-89 0.005035848753578875 2305 90-91 -0.05448221697848865 2305 92-93 -0.0030151064906434044 2305 94-95 -0.10212976168928378 2305 96-97 0.14903121719826018 2305 98-99 -0.13631663106406933 2305 100-101 -0.07035637690398389 2306 1 -0.2263679935899141 2306 2 -0.2718476828163041 2306 3 -0.22933501957339786 2306 4 -0.19985290349826101 2306 5 -0.17905589575095604 2306 6 -0.1587338729469181 2306 7 -0.21039222377539346 2306 8 -0.17335113504716304 2306 9 -0.3376669975298441 2306 10-11 -0.22453336564648652 2306 12-13 -0.28899940785340306 2306 14-15 -0.3514813186552672 2306 16-17 -0.3883927078865099 2306 18-19 -0.3796901873414811 2306 20-21 -0.4345257788760577 2306 22-23 -0.3753362933767903 2306 24-25 -0.37768624687626584 2306 26-27 -0.4143908445686364 2306 28-29 -0.5278201818857724 2306 30-31 -0.5156122601322437 2306 32-33 -0.5968143904772347 2306 34-35 -0.5724557921779478 2306 36-37 -0.5669813455845159 2306 38-39 -0.776777488536375 2306 40-41 -0.6072676759274813 2306 42-43 -0.7084838625988965 2306 44-45 -0.7094303217908404 2306 46-47 -0.5924564401978998 2306 48-49 -0.5245862276693529 2306 50-51 -0.5669142090262795 2306 52-53 -0.4910557124813124 2306 54-55 -0.5398560003421409 2306 56-57 -0.5333269802247216 2306 58-59 -0.5600613657329916 2306 60-61 -0.5447067767805507 2306 62-63 -0.4630879485651249 2306 64-65 -0.5270136087479003 2306 66-67 -0.4921987856847494 2306 68-69 -0.5181101462690023 2306 70-71 -0.6063574201897026 2306 72-73 -0.626821566551989 2306 74-75 -0.6095400295582891 2306 76-77 -0.5793367046021345 2306 78-79 -0.4816890936937597 2306 80-81 -0.4953846105456208 2306 82-83 -0.4528797495162351 2306 84-85 -0.4848902994396873 2306 86-87 -0.45864495559846574 2306 88-89 -0.45336024258803675 2306 90-91 -0.48141307062221017 2306 92-93 -0.5065523438660549 2306 94-95 -0.5090853281448204 2306 96-97 -0.2610793876606401 2306 98-99 -0.5562586023604972 2306 100-101 -0.5613774350069711 2307 1 -0.443453105717186 2307 2 -0.46523722431252423 2307 3 -0.47431349732925554 2307 4 -0.3605776322887948 2307 5 -0.3819837700270057 2307 6 -0.36986881565675844 2307 7 -0.4223909710398672 2307 8 -0.388761844397429 2307 9 -0.4707778020452622 2307 10-11 -0.5722897536600229 2307 12-13 -0.5700171215786369 2307 14-15 -0.8940016896409517 2307 16-17 -0.809372535177161 2307 18-19 -0.6740853393281796 2307 20-21 -0.6300077936929114 2307 22-23 -0.5842606368912797 2307 24-25 -0.6223324033528641 2307 26-27 -0.6610470785640672 2307 28-29 -0.6771942507177826 2307 30-31 -0.7172800599352271 2307 32-33 -0.7711968595978291 2307 34-35 -0.7854493702035867 2307 36-37 -0.8590207119501514 2307 38-39 -0.778875292469543 2307 40-41 -0.7306040580773541 2307 42-43 -0.8334606679894208 2307 44-45 -0.7359931112296891 2307 46-47 -0.757978258723476 2307 48-49 -0.6879777450681317 2307 50-51 -0.7557028872044356 2307 52-53 -0.8613278094815016 2307 54-55 -0.8639010421053257 2307 56-57 -0.8963246913461873 2307 58-59 -0.9112874960130739 2307 60-61 -1.0196027809791772 2307 62-63 -0.9569220806102194 2307 64-65 -0.8940503441111431 2307 66-67 -0.8472046009173155 2307 68-69 -0.8557257050712437 2307 70-71 -0.9156849692401678 2307 72-73 -1.2373771544247845 2307 74-75 -0.9873068222455714 2307 76-77 -0.859818061824317 2307 78-79 -0.8372504839091945 2307 80-81 -0.7877377824201588 2307 82-83 -0.8194083115993038 2307 84-85 -0.8151953306439381 2307 86-87 -0.7586263961686406 2307 88-89 -0.8247168947039043 2307 90-91 -0.905361816242177 2307 92-93 -0.858800793736819 2307 94-95 -0.8687832799214057 2307 96-97 -0.6619252024374909 2307 98-99 -0.9736940151497002 2307 100-101 -1.0635314619333833 2308 1 -1.3829298186250085 2308 2 -0.867731981632204 2308 3 -0.8496564536457072 2308 4 -0.5228875246271656 2308 5 -0.5948383476713985 2308 6 -0.6460046052455084 2308 7 -0.6064107332183895 2308 8 -0.5695985600267903 2308 9 -0.8353222676102732 2308 10-11 -0.7961911321744779 2308 12-13 -0.7687449905262582 2308 14-15 -0.9720045579031122 2308 16-17 -0.9000261674536958 2308 18-19 -0.8700480930314995 2308 20-21 -0.909001557362636 2308 22-23 -0.972599831813703 2308 24-25 -1.1446284048036546 2308 26-27 -1.1471714992256565 2308 28-29 -1.150262149770704 2308 30-31 -1.000701487719759 2308 32-33 -1.0773574049368264 2308 34-35 -1.158900995423842 2308 36-37 -1.2182846125095566 2308 38-39 -1.215420509946476 2308 40-41 -1.1124659442721523 2308 42-43 -1.084565816107073 2308 44-45 -1.1357525528942674 2308 46-47 -1.1384114967188665 2308 48-49 -1.2434852914255998 2308 50-51 -1.230341534785694 2308 52-53 -1.202907714540146 2308 54-55 -1.1429432201265328 2308 56-57 -1.0823699861648706 2308 58-59 -1.027626775172827 2308 60-61 -0.9561833648665328 2308 62-63 -1.0133921936225434 2308 64-65 -1.0369235296386208 2308 66-67 -0.9910577844773485 2308 68-69 -1.0947181770091419 2308 70-71 -0.994060364101621 2308 72-73 -1.019681299281281 2308 74-75 -1.138829322669551 2308 76-77 -1.0679786442126655 2308 78-79 -1.4733509875085318 2308 80-81 -1.1490310954013552 2308 82-83 -1.0761141282039475 2308 84-85 -0.9969278438314291 2308 86-87 -1.0102693925776691 2308 88-89 -1.0594689475444738 2308 90-91 -1.141848098710195 2308 92-93 -1.0464367355522342 2308 94-95 -1.1778373704896197 2308 96-97 -1.041416832788311 2308 98-99 -1.3152576353624248 2308 100-101 -1.3449664701902115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38.0 3 93.0 4 205.0 5 622.0 6 1195.0 7 2137.0 8 4204.0 9 6278.0 10 8491.0 11 10328.0 12 12239.0 13 14234.0 14 15976.0 15 17671.0 16 20406.0 17 23392.0 18 27418.0 19 31387.0 20 33820.0 21 35363.0 22 38267.0 23 44685.0 24 54664.0 25 69165.0 26 88241.0 27 112632.0 28 143530.0 29 180350.0 30 222279.0 31 274397.0 32 337985.0 33 424048.0 34 559213.0 35 828033.0 36 1125524.0 37 1926903.0 38 2285679.0 39 636113.0 40 2152.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.334296956949053 31.323575938098248 28.00575450169569 22.33637260325701 2 22.99020473799673 28.955180024473858 28.037217982140884 20.017397255388527 3 21.117583268365138 28.084828381559507 28.631652837684996 22.16593551239036 4 21.665003691534505 28.623125202587378 28.21588168626718 21.495989419610936 5 22.45404233660537 28.456773515877682 28.141459118387974 20.947725029128975 6 22.172240826491834 28.282108669009787 27.962222422974843 21.583428081523536 7 22.350319257305866 28.595112958173814 27.890190581345507 21.164377203174805 8 21.589396289778495 28.427066483509 28.209302511750323 21.774234714962184 9 21.34278748433983 28.255366045111685 28.676051505715638 21.725794964832843 10-11 21.434239315580033 28.74798700162599 28.09407115257288 21.723702530221097 12-13 21.436905813974885 28.80628611662921 27.902842154626345 21.853965914769564 14-15 21.632511403828758 28.82509194741395 27.715308829893726 21.827087818863568 16-17 21.819498954036117 28.61376805123253 27.64895304332712 21.91777995140424 18-19 21.773419990546145 28.664888931765397 27.629762571448385 21.931928506240077 20-21 21.914869153936174 28.568785834645706 27.665274300558757 21.851070710859364 22-23 21.83044574082509 28.563804055067987 27.73993303390548 21.865817170201446 24-25 21.787947651750635 28.54584869416832 27.63195936661708 22.03424428746397 26-27 21.964557627396967 28.385492839483405 27.817140691120496 21.83280884199913 28-29 21.961119646562654 28.47136248296014 27.706987067846633 21.86053080263057 30-31 21.9488007358496 28.488333471769472 27.66280532056353 21.900060471817397 32-33 21.937765694734065 28.535285674499868 27.59665744810178 21.930291182664288 34-35 21.980544021809358 28.561404883923114 27.558655947585684 21.89939514668184 36-37 21.955292853773905 28.561186574113012 27.54730903531286 21.936211536800222 38-39 21.95079671125627 28.543685404336276 27.545739283821153 21.959778600586297 40-41 21.967419443939963 28.63220483448114 27.525571615649568 21.87480410592933 42-43 22.012989017873025 28.612655710771524 27.450124784847883 21.92423048650757 44-45 21.963848519188964 28.636077234684187 27.489987116602492 21.910087129524353 46-47 21.99024435782165 28.677256113753558 27.406843690635647 21.925655837789147 48-49 22.003456156165115 28.671526589563108 27.41191537022693 21.913101884044845 50-51 22.029341462220394 28.649336956721744 27.394159505671738 21.92716207538612 52-53 21.973287819549686 28.73079770300655 27.337908344601413 21.95800613284235 54-55 22.040121808557476 28.73079250515393 27.27872559465253 21.95036009163606 56-57 22.01810370485262 28.741042670523615 27.25244525179801 21.988408372825752 58-59 21.99743704282937 28.822732122323767 27.245781604737196 21.934049230109665 60-61 21.977590384717143 28.801001481076742 27.229123414017554 21.99228472018856 62-63 21.97667419022159 28.84783885222117 27.22388724096533 21.951599716591915 64-65 21.975929177964456 28.921724996744835 27.18370758439589 21.918638240894825 66-67 21.960316791195396 28.920965726002002 27.166140313470798 21.952577169331803 68-69 21.939478787563136 28.97430967454409 27.17841377897599 21.90779775891678 70-71 21.954466019432616 29.057580696645253 27.10915251129144 21.878800772630687 72-73 21.933963649428208 28.99221345800422 27.119026242252144 21.954796650315426 74-75 21.99728443218259 29.035124095170247 27.09259699536902 21.874994477278147 76-77 21.963790858054537 29.12520734000263 27.046223081189012 21.86477872075382 78-79 21.96411382188322 29.08867676160561 27.035612257278807 21.91159715923236 80-81 21.920052910022658 29.18428112004871 26.97896567746083 21.9167002924678 82-83 21.959653804180164 29.229386568265454 26.95764088668171 21.853318740872673 84-85 21.927595589285293 29.264056243323633 26.942894210395014 21.865453956996056 86-87 21.90375095830023 29.324309612727017 26.891835042679357 21.880104386293397 88-89 21.910244116317088 29.380966230603896 26.899778359972053 21.809011293106966 90-91 21.875035299535 29.3725810546296 26.875307805438386 21.877075840397016 92-93 21.85888554775031 29.457858970082583 26.83793506808802 21.84532041407909 94-95 21.869889956089008 29.505778616662198 26.828136422144993 21.7961950051038 96-97 21.835466865191684 29.521779935562854 26.812237533172638 21.830515666072824 98-99 21.835274688788132 29.56383340798718 26.74433163515123 21.85656026807346 100-101 21.866806548727432 29.641906779043907 26.677357206908248 21.813929465320413 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 151790.0 1 85694.5 2 14652.0 3 8871.0 4 7646.5 5 6766.5 6 6305.0 7 6241.5 8 5968.5 9 5526.0 10 5225.5 11 5797.5 12 10568.5 13 10590.5 14 6368.0 15 6409.0 16 6929.5 17 8251.0 18 10265.5 19 12091.5 20 14996.0 21 19540.0 22 25074.5 23 31727.0 24 38959.0 25 47840.0 26 60190.0 27 74158.5 28 85629.0 29 96308.0 30 111588.5 31 130939.0 32 150574.5 33 170622.5 34 191662.5 35 212264.0 36 233640.0 37 254161.5 38 268960.5 39 275203.5 40 284922.5 41 299240.0 42 310737.5 43 326387.0 44 340400.5 45 351888.0 46 359859.0 47 364193.5 48 374312.5 49 381346.0 50 378307.5 51 368161.0 52 352500.0 53 338060.5 54 319268.5 55 294308.5 56 267822.5 57 240110.0 58 213851.5 59 181850.5 60 145773.5 61 110785.5 62 78435.0 63 53299.0 64 35032.0 65 21338.5 66 12119.0 67 7120.5 68 3923.0 69 1871.5 70 894.5 71 435.5 72 222.5 73 134.0 74 95.0 75 71.5 76 46.0 77 31.5 78 24.5 79 17.5 80 12.5 81 12.0 82 9.5 83 5.0 84 5.5 85 4.0 86 1.5 87 2.5 88 2.5 89 1.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011934269619060817 2 0.01817169276491142 3 0.005696846473210215 4 5.30180967397301E-4 5 7.173036617728191E-4 6 0.0 7 0.0 8 0.007131453796755853 9 0.010468475179785925 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.1955061029546986E-4 24-25 0.004865190053763469 26-27 0.006991111775974216 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 5.197852621542167E-6 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 4.1582820972337336E-5 62-63 1.299463155385542E-4 64-65 3.5865183088640957E-4 66-67 2.49496925834024E-4 68-69 3.690475361294939E-4 70-71 1.4034202078163854E-4 72-73 3.638496835079517E-5 74-75 6.2374231458506E-5 76-77 0.12421828194961472 78-79 0.05473858595746057 80-81 7.796778932313251E-5 82-83 0.002437792879503277 84-85 0.010463277327164382 86-87 0.11642150301730146 88-89 0.13295067435380556 90-91 0.1461688135703873 92-93 0.14397012191147496 94-95 0.11257509207736026 96-97 1.2122275948381995 98-99 0.07501021118147502 100-101 0.06754089696431892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9619357.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.77018259868662 #Duplication Level Percentage of deduplicated Percentage of total 1 90.27946718822794 56.66858640317205 2 5.406312931218969 6.787104997565107 3 1.4036057883748574 2.643137748985898 4 0.6536059240460159 1.6410785279980684 5 0.40416068521893683 1.2684620005201486 6 0.23264617169318166 0.8761945606839847 7 0.1648653632892671 0.724404027050627 8 0.12260057722771935 0.6156528495430659 9 0.10180280068793462 0.5751162349415421 >10 1.115035500881957 15.426994026116311 >50 0.10863048759684238 4.48239979455365 >100 0.006689304183213073 0.5538802968342761 >500 1.2828385626855777E-4 0.044570039991310324 >1k 1.2828385626844973E-4 0.1507812102685815 >5k 1.2828385626844973E-4 0.481614108740393 >10k+ 1.9242578440267457E-4 7.06002317303506 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133035 1.3829926470137244 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29088 0.30239027411083713 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 13229 0.13752478466076268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0395705243084336E-5 1.0395705243084336E-5 0.0 0.0 0.0 2 4.158282097233734E-5 1.0395705243084336E-5 0.0 0.0 1.0395705243084336E-5 3 7.276993670159035E-5 2.079141048616867E-5 0.0 0.0 1.0395705243084336E-5 4 1.455398734031807E-4 3.118711572925301E-5 0.0 0.0 1.0395705243084336E-5 5 1.6633128388934937E-4 4.158282097233734E-5 0.0 0.0 2.079141048616867E-5 6 1.6633128388934937E-4 4.158282097233734E-5 0.0 0.0 2.079141048616867E-5 7 2.2870551534785537E-4 4.158282097233734E-5 0.0 1.0395705243084336E-5 2.079141048616867E-5 8 2.8068404156327703E-4 5.1978526215421676E-5 0.0 1.0395705243084336E-5 2.079141048616867E-5 9 3.014754520494457E-4 5.1978526215421676E-5 0.0 1.0395705243084336E-5 2.079141048616867E-5 10-11 3.482561256433252E-4 5.1978526215421676E-5 0.0 1.5593557864626504E-5 2.598926310771084E-5 12-13 3.950367992372047E-4 5.1978526215421676E-5 0.0 2.079141048616867E-5 5.717637883696384E-5 14-15 4.4181747283108424E-4 5.1978526215421676E-5 0.0 2.079141048616867E-5 8.316564194467468E-5 16-17 5.041917042895903E-4 6.237423145850602E-5 0.0 2.079141048616867E-5 9.356134718775902E-5 18-19 6.18544461963518E-4 7.276993670159035E-5 0.0 2.079141048616867E-5 1.0395705243084335E-4 20-21 6.445337250712287E-4 7.276993670159035E-5 0.0 2.079141048616867E-5 1.0395705243084335E-4 22-23 6.861165460435661E-4 7.276993670159035E-5 0.0 2.079141048616867E-5 1.1435275767392769E-4 24-25 7.692821879882408E-4 7.276993670159035E-5 0.0 2.079141048616867E-5 1.2474846291701203E-4 26-27 8.628435351759997E-4 7.276993670159035E-5 0.0 2.079141048616867E-5 1.299463155385542E-4 28-29 9.200199140129636E-4 7.796778932313251E-5 0.0 2.598926310771084E-5 1.6633128388934937E-4 30-31 9.97987703336096E-4 1.0395705243084335E-4 0.0 3.118711572925301E-5 2.0271625224014454E-4 32-33 0.001086351197902313 1.1435275767392769E-4 0.0 3.118711572925301E-5 2.1311195748322887E-4 34-35 0.0012007039555762408 1.1435275767392769E-4 0.0 3.118711572925301E-5 2.1830981010477103E-4 36-37 0.0013410459763578792 1.1955061029546986E-4 0.0 3.118711572925301E-5 2.3390336796939753E-4 38-39 0.0014761901445179755 1.2474846291701203E-4 0.0 3.118711572925301E-5 2.442990732124819E-4 40-41 0.0015801471969488189 1.2474846291701203E-4 0.0 3.118711572925301E-5 2.4949692583402407E-4 42-43 0.0016893021020012045 1.2474846291701203E-4 0.0 3.118711572925301E-5 2.4949692583402407E-4 44-45 0.0017776655965674212 1.2474846291701203E-4 0.0 3.118711572925301E-5 2.598926310771084E-4 46-47 0.0018244462701613008 1.2474846291701203E-4 0.0 3.118711572925301E-5 2.702883363201927E-4 48-49 0.0018816226489982647 1.299463155385542E-4 0.0 3.118711572925301E-5 2.858818941848192E-4 50-51 0.002047953932887614 1.3514416816009635E-4 0.0 3.118711572925301E-5 3.170690099140722E-4 52-53 0.002193493806290795 1.455398734031807E-4 0.0 3.118711572925301E-5 3.482561256433252E-4 54-55 0.0023026487113431806 1.455398734031807E-4 0.0 3.118711572925301E-5 3.638496835079517E-4 56-57 0.002474177847854072 1.5073772602472286E-4 0.0 3.6384968350795175E-5 3.794432413725782E-4 58-59 0.002692487657958843 1.5593557864626502E-4 0.0 4.158282097233734E-5 4.158282097233734E-4 60-61 0.00285362108922665 1.6633128388934937E-4 0.0 4.158282097233734E-5 4.2622391496645773E-4 62-63 0.003051139488845252 1.6633128388934937E-4 0.0 4.158282097233734E-5 4.3661962020954205E-4 64-65 0.0032694492989500234 1.6633128388934937E-4 0.0 4.158282097233734E-5 4.3661962020954205E-4 66-67 0.0033941977618670354 1.7152913651089153E-4 0.0 4.158282097233734E-5 4.3661962020954205E-4 68-69 0.003591716161485638 1.767269891324337E-4 0.0 4.158282097233734E-5 4.7300458856033725E-4 70-71 0.0038620044978058305 1.767269891324337E-4 0.0 4.158282097233734E-5 4.782024411818794E-4 72-73 0.004064720750045976 1.767269891324337E-4 0.0 4.158282097233734E-5 5.249831147757589E-4 74-75 0.004381789759960047 1.767269891324337E-4 0.0 4.158282097233734E-5 5.405766726403854E-4 76-77 0.0048184093801695894 1.9751839961860236E-4 0.0 4.158282097233734E-5 5.561702305050119E-4 78-79 0.005208248326785252 2.079141048616867E-4 0.0 4.158282097233734E-5 5.873573462342649E-4 80-81 0.005800803525641059 2.079141048616867E-4 0.0 4.158282097233734E-5 5.977530514773492E-4 82-83 0.00652330504003542 2.1830981010477103E-4 0.0 4.158282097233734E-5 6.133466093419758E-4 84-85 0.007516094890749975 2.2870551534785537E-4 0.0 4.158282097233734E-5 6.393358724496866E-4 86-87 0.008976691477403324 2.2870551534785537E-4 0.0 4.158282097233734E-5 6.549294303143131E-4 88-89 0.010702378547755322 2.2870551534785537E-4 0.0 4.158282097233734E-5 6.549294303143131E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 290 0.0 27.796824 3 CGCGTAG 170 0.0 23.704248 54-55 CAACGCA 7535 0.0 22.304329 12-13 ATCAACG 7895 0.0 21.257263 10-11 AGTGGTA 9785 0.0 19.237822 5 GGTATCA 9675 0.0 19.211401 8 TCGCGTA 215 0.0 18.742895 52-53 GTATCAA 9960 0.0 18.708311 9 ACGCAGA 9210 0.0 18.273636 14-15 ACGTACG 245 0.0 17.418175 20-21 CGTACGC 260 0.0 17.325129 20-21 TGGTATC 10875 0.0 17.309526 7 TCAACGC 7520 0.0 16.86406 10-11 GTGGTAT 11305 0.0 16.735022 6 AACGCAG 7975 0.0 15.8424635 12-13 AAGCAGT 12545 0.0 15.688626 1 AGAGTAC 11095 0.0 14.913718 18-19 TATCAAC 10730 0.0 14.361011 9 GCAGTGG 14990 0.0 13.032863 3 CGCAGAG 9950 0.0 12.745501 14-15 >>END_MODULE