FastQCFastQC Report
Sat 18 Jun 2016
SRR578553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9308442
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108635611.670653370349195No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT264850.2845266694469386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109420.11754920963142917No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG104090.11182322455250836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2413950.091.3327941
AGTGGTA2405600.091.176825
GTATCAA2413700.091.018239
GGTATCA2415100.090.97928
TGGTATC2416050.090.863727
GTGGTAT2420650.090.741096
AGCAGTG2443750.090.1655042
GCAGTGG2441600.090.1191943
CAGTGGT2438800.089.857294
TCAACGC2408400.046.1777912-13
CAACGCA2411100.046.0354412-13
AACGCAG2421050.046.024814-15
ATCAACG2410800.045.99843210-11
CGCAGAG2428700.045.8897916-17
ACGCAGA2421950.045.85469414-15
TATCAAC2423000.045.7756510-11
AGAGTAC2364200.045.55681618-19
CAGAGTA2422800.045.4283218-19
GAGTACT1401400.045.1936920-21
GCAGAGT2449700.045.13573516-17
Produced by FastQC (version 0.11.5)