##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578553_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9308442 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.527319609446995 31.0 31.0 34.0 27.0 34.0 2 30.82622580663875 31.0 31.0 34.0 27.0 34.0 3 30.901182389061457 31.0 31.0 34.0 27.0 34.0 4 34.286844243107495 37.0 35.0 37.0 30.0 37.0 5 34.39121971217095 37.0 35.0 37.0 30.0 37.0 6 34.363561163081854 37.0 35.0 37.0 30.0 37.0 7 34.35463969158319 37.0 35.0 37.0 30.0 37.0 8 34.30622095512869 37.0 35.0 37.0 30.0 37.0 9 35.69421510065809 39.0 35.0 39.0 30.0 39.0 10-11 35.66562572984824 39.0 35.0 39.0 30.0 39.0 12-13 35.70263068728365 39.0 35.0 39.0 30.0 39.0 14-15 36.75088956884514 40.0 36.0 41.0 30.0 41.0 16-17 36.71081981281078 40.0 36.0 41.0 30.0 41.0 18-19 36.71157326865226 40.0 36.0 41.0 30.0 41.0 20-21 36.602381687504746 39.5 36.0 41.0 30.0 41.0 22-23 36.305797683436175 39.0 36.0 40.5 28.5 41.0 24-25 36.20306040473798 39.0 36.0 40.5 29.0 41.0 26-27 36.09177942989815 39.0 36.0 40.5 27.5 41.0 28-29 35.81766024862163 39.0 35.0 41.0 27.0 41.0 30-31 35.58148903973404 39.0 34.5 40.5 26.5 41.0 32-33 35.1961891689286 38.0 34.0 40.0 25.0 41.0 34-35 34.98278396105385 38.0 34.0 40.0 25.0 41.0 36-37 34.6577062520237 38.0 33.5 40.0 24.0 41.0 38-39 34.011962528208265 37.0 33.0 40.0 24.0 41.0 40-41 34.18736927189319 37.0 33.0 40.0 24.0 41.0 42-43 34.12028581152464 37.0 33.0 40.0 23.0 41.0 44-45 33.98107798276017 37.0 33.0 40.0 23.0 41.0 46-47 33.77386650741337 37.0 33.0 40.0 22.5 41.0 48-49 33.425049809624426 37.0 33.0 40.0 20.0 41.0 50-51 32.214716007254495 36.0 31.5 39.0 14.0 40.5 52-53 31.830551987110194 36.5 31.0 39.0 6.5 40.0 54-55 31.453427329729294 37.0 30.5 40.0 2.0 41.0 56-57 30.97241659775073 37.0 29.5 40.0 2.0 41.0 58-59 30.636709129196916 36.0 29.0 40.0 2.0 41.0 60-61 30.332783456135836 36.0 28.0 40.0 2.0 41.0 62-63 29.99691650869179 35.5 28.0 39.0 2.0 41.0 64-65 29.663159312804442 35.0 28.0 39.0 2.0 41.0 66-67 29.347675045942168 35.0 27.5 39.0 2.0 40.5 68-69 29.02887932266216 35.0 26.5 38.0 2.0 40.0 70-71 28.655839613116783 35.0 26.0 37.5 2.0 40.0 72-73 28.260349368884718 34.5 26.0 37.0 2.0 39.5 74-75 27.89495540714547 34.0 26.0 36.5 2.0 39.0 76-77 27.500280605497675 34.0 25.0 36.0 2.0 39.0 78-79 27.13857893727006 34.0 24.5 35.5 2.0 38.0 80-81 26.777171195781207 34.0 23.5 35.0 2.0 37.0 82-83 26.401135549858935 34.0 22.5 35.0 2.0 37.0 84-85 26.053181671003593 33.0 20.5 35.0 2.0 36.0 86-87 25.71340714160329 33.0 19.5 35.0 2.0 36.0 88-89 25.35144082113849 33.0 18.0 35.0 2.0 35.5 90-91 24.98886811563095 32.5 14.5 35.0 2.0 35.0 92-93 24.636378461615813 32.0 7.0 35.0 2.0 35.0 94-95 24.206285863950164 32.0 2.0 35.0 2.0 35.0 96-97 23.72015703594651 32.0 2.0 35.0 2.0 35.0 98-99 23.206124881048837 32.0 2.0 35.0 2.0 35.0 100-101 21.88250386047418 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.517620469413675 1101 2 0.5777107829716215 1101 3 0.627759321224282 1101 4 0.6605871127718856 1101 5 0.6480309208914434 1101 6 0.49214668518114735 1101 7 0.44061753903207546 1101 8 0.4620287517038122 1101 9 0.588251136953474 1101 10-11 0.6556741269112152 1101 12-13 0.7302448019921144 1101 14-15 0.8102387307380567 1101 16-17 0.7268782087738472 1101 18-19 0.7879032042973151 1101 20-21 0.7858837790035338 1101 22-23 0.7505616530020163 1101 24-25 0.7742996421850137 1101 26-27 0.9778386715584304 1101 28-29 1.0066507651563015 1101 30-31 1.0845673076879265 1101 32-33 1.0343157303246855 1101 34-35 1.1379435198215262 1101 36-37 1.1154612240851023 1101 38-39 1.07583722377192 1101 40-41 1.179470727895442 1101 42-43 1.3732236116844305 1101 44-45 1.3366316972972783 1101 46-47 1.2982525724619833 1101 48-49 1.205688097301703 1101 50-51 1.1676863583724924 1101 52-53 1.242874059455108 1101 54-55 1.1559313959434299 1101 56-57 1.2303756675075022 1101 58-59 1.2029926198132195 1101 60-61 1.2530073687271255 1101 62-63 1.2890001135990694 1101 64-65 1.1119271288202732 1101 66-67 1.2385619832701629 1101 68-69 1.2130981292259904 1101 70-71 1.2083793316409412 1101 72-73 1.3067972291681968 1101 74-75 1.231180701020353 1101 76-77 1.2558936443583555 1101 78-79 1.2135368692710422 1101 80-81 1.2621631114733134 1101 82-83 1.2932347858218165 1101 84-85 1.312094052432677 1101 86-87 1.242020224751137 1101 88-89 1.3060394592327746 1101 90-91 1.3002357093708916 1101 92-93 1.4352553312569754 1101 94-95 1.535173659724883 1101 96-97 1.6578880734559505 1101 98-99 1.783577345966986 1101 100-101 2.1148999128733337 1102 1 0.4476122987688882 1102 2 0.46160742721247416 1102 3 0.4818554798622756 1102 4 0.6180692793255531 1102 5 0.5077237973640436 1102 6 0.47459926936486596 1102 7 0.37121260535337797 1102 8 0.5486658649744456 1102 9 0.5428131961877511 1102 10-11 0.6559407449016987 1102 12-13 0.5930826813331294 1102 14-15 0.598508756061257 1102 16-17 0.6060131055397733 1102 18-19 0.5783243112168677 1102 20-21 0.7862003480280961 1102 22-23 0.6820073935463782 1102 24-25 0.6798139581977409 1102 26-27 0.8087381378588674 1102 28-29 0.6868274481063139 1102 30-31 0.8898861656519941 1102 32-33 0.844048069273164 1102 34-35 0.8335772663229264 1102 36-37 0.8057700280997224 1102 38-39 0.7889420108425966 1102 40-41 0.8730333608132881 1102 42-43 0.934926640617789 1102 44-45 0.8660676330678996 1102 46-47 0.980520940477092 1102 48-49 0.7784136157899582 1102 50-51 0.8468340616100392 1102 52-53 0.9150794961210202 1102 54-55 0.7534099697313046 1102 56-57 0.7217631704773311 1102 58-59 0.5660903951958218 1102 60-61 0.7954177668867075 1102 62-63 0.9458406817196483 1102 64-65 0.7364726729214723 1102 66-67 0.8222295597178437 1102 68-69 0.8514620879903099 1102 70-71 0.7726805676682709 1102 72-73 0.9521630828267291 1102 74-75 0.9892162694016982 1102 76-77 0.9019292011501285 1102 78-79 0.8112636336377612 1102 80-81 0.8427154122921188 1102 82-83 0.8259275637830967 1102 84-85 0.8476816145092947 1102 86-87 0.8988387726942797 1102 88-89 0.9739608124306294 1102 90-91 1.0722553073288132 1102 92-93 1.2039618776239323 1102 94-95 1.1812063770727264 1102 96-97 1.2486033235877798 1102 98-99 1.4677672062547522 1102 100-101 1.7423278982009371 1103 1 0.27336664326885796 1103 2 0.36430001935578815 1103 3 0.33227113308713285 1103 4 0.40644110714321613 1103 5 0.3937074708653725 1103 6 0.27269561057672576 1103 7 0.17491656164759917 1103 8 0.2982931438559291 1103 9 0.36443694658674275 1103 10-11 0.39904326598902173 1103 12-13 0.3983864176003067 1103 14-15 0.5564326924434724 1103 16-17 0.47403296275697926 1103 18-19 0.48513371782024706 1103 20-21 0.5108376751467105 1103 22-23 0.43014169165954286 1103 24-25 0.44757323206377464 1103 26-27 0.6601003098656761 1103 28-29 0.5931481106918213 1103 30-31 0.6580913073307428 1103 32-33 0.690223534133402 1103 34-35 0.76543308488111 1103 36-37 0.74959219302945 1103 38-39 0.7898287433124054 1103 40-41 0.650156222104016 1103 42-43 0.8312684394395049 1103 44-45 0.7846801545968418 1103 46-47 0.669190665683665 1103 48-49 0.8214200986498241 1103 50-51 0.808650117658793 1103 52-53 0.7256647758868588 1103 54-55 0.6841657089657183 1103 56-57 0.6502887600810539 1103 58-59 0.6388684490633167 1103 60-61 0.6706278854475158 1103 62-63 0.7390498902092482 1103 64-65 0.778543817235402 1103 66-67 0.7682074977580946 1103 68-69 0.7156910066770124 1103 70-71 0.7288028649160871 1103 72-73 0.7470028169383554 1103 74-75 0.6318216612941221 1103 76-77 0.7171185580126398 1103 78-79 0.7496093276537046 1103 80-81 0.8146976569592859 1103 82-83 0.7525570692085886 1103 84-85 0.8078977228815596 1103 86-87 0.8700579662526735 1103 88-89 0.8539447799721671 1103 90-91 0.9609729677973426 1103 92-93 0.9211889163539482 1103 94-95 0.9470738991019836 1103 96-97 1.0140233287195102 1103 98-99 1.0970422070792978 1103 100-101 1.3167981646427371 1104 1 0.23764415667299588 1104 2 0.205314335830991 1104 3 0.2931676137160295 1104 4 0.37307912377315233 1104 5 0.21513767250631588 1104 6 0.22251822741797866 1104 7 0.22943963153289104 1104 8 0.24489598886159314 1104 9 0.3462888357316345 1104 10-11 0.40186391533831056 1104 12-13 0.43251630572567024 1104 14-15 0.45120031411197914 1104 16-17 0.5144780777135267 1104 18-19 0.44129360395669437 1104 20-21 0.4852203463822562 1104 22-23 0.41630809144928804 1104 24-25 0.4612680344983602 1104 26-27 0.4473028161746555 1104 28-29 0.4071237414248614 1104 30-31 0.5180201503598809 1104 32-33 0.5453017010174364 1104 34-35 0.609554425600976 1104 36-37 0.6320962446192269 1104 38-39 0.6889643321990846 1104 40-41 0.7017704118931718 1104 42-43 0.7484366736471557 1104 44-45 0.8281492885876105 1104 46-47 0.7611410810999715 1104 48-49 0.9115882906214168 1104 50-51 0.8300951655801185 1104 52-53 0.7402202142061292 1104 54-55 0.7921989139283632 1104 56-57 0.7769951260631203 1104 58-59 0.7810988153972218 1104 60-61 0.7488362305738256 1104 62-63 0.7910247029022734 1104 64-65 0.8948617725272818 1104 66-67 0.8017964819730423 1104 68-69 0.6882059527352524 1104 70-71 0.7447763721106924 1104 72-73 0.721995170993246 1104 74-75 0.791867132317833 1104 76-77 0.8921511664116615 1104 78-79 0.7708236050370267 1104 80-81 0.7018352426208558 1104 82-83 0.7557253163693751 1104 84-85 0.8473683638639038 1104 86-87 0.8483653862210119 1104 88-89 0.9205929044723433 1104 90-91 0.896052796446245 1104 92-93 1.0191472317406465 1104 94-95 1.0638103512207095 1104 96-97 1.0222581375343438 1104 98-99 1.2095342670593787 1104 100-101 1.4804749572439064 1105 1 -0.04230795838360635 1105 2 0.043874399299170364 1105 3 0.14886846315309654 1105 4 0.2381586565729421 1105 5 0.2924156839647267 1105 6 0.18618369823455794 1105 7 0.23200467648479162 1105 8 0.30832528783641067 1105 9 0.2285705989122775 1105 10-11 0.11009819789266828 1105 12-13 0.1769290776927832 1105 14-15 0.028068616108917865 1105 16-17 0.13820831567772274 1105 18-19 0.10195703113802779 1105 20-21 0.35375876622278923 1105 22-23 0.3648513272916887 1105 24-25 0.36821856110393014 1105 26-27 0.6207865942604514 1105 28-29 0.5742131636203425 1105 30-31 0.5210161903663177 1105 32-33 0.5823869730680613 1105 34-35 0.6150627386620542 1105 36-37 0.6221688459566579 1105 38-39 0.7095770985773697 1105 40-41 0.570254045891879 1105 42-43 0.6284578686400053 1105 44-45 0.6622546594776111 1105 46-47 0.6872326117698861 1105 48-49 0.8259218998305897 1105 50-51 0.7717146237122279 1105 52-53 0.6425814086706545 1105 54-55 0.6696100367532161 1105 56-57 0.6800836983630738 1105 58-59 0.7583592853438219 1105 60-61 0.572203658333251 1105 62-63 0.5717752405545262 1105 64-65 0.7014386769383769 1105 66-67 0.85804819710064 1105 68-69 0.7295808666923307 1105 70-71 0.566260794440435 1105 72-73 0.734000971371934 1105 74-75 0.6073202327336595 1105 76-77 0.4942435074623148 1105 78-79 0.6150477811917909 1105 80-81 0.537490128275742 1105 82-83 0.532727532035878 1105 84-85 0.6447539254204209 1105 86-87 0.748801293335486 1105 88-89 0.7683628292610649 1105 90-91 0.8012243714251319 1105 92-93 0.9387912252846675 1105 94-95 1.0191399526256006 1105 96-97 1.0504775671110167 1105 98-99 1.0915584418169324 1105 100-101 1.328463015698727 1106 1 0.00914802030723294 1106 2 0.1443652285307877 1106 3 0.18617733562697936 1106 4 0.2854052056137846 1106 5 0.21748039326526225 1106 6 0.1582821713903826 1106 7 0.1298139749509275 1106 8 0.06230584518127813 1106 9 0.017333240734835442 1106 10-11 0.13134890538175625 1106 12-13 0.1618582333044074 1106 14-15 0.13969044423186716 1106 16-17 0.1547741297603622 1106 18-19 0.045718514998839055 1106 20-21 0.15159431034157222 1106 22-23 0.27226089469805714 1106 24-25 0.2721101729977846 1106 26-27 0.15931166518699058 1106 28-29 0.10318826977423612 1106 30-31 0.23993223380976048 1106 32-33 0.23732959900555528 1106 34-35 0.17897508284514885 1106 36-37 0.24849183113096984 1106 38-39 0.49112465616625656 1106 40-41 0.1593815733404611 1106 42-43 0.06065803703850037 1106 44-45 0.16687125201474373 1106 46-47 0.18700843570463377 1106 48-49 0.1134375400857337 1106 50-51 0.28352044895925843 1106 52-53 0.3045683111574 1106 54-55 0.20119065675902092 1106 56-57 0.2230071976146739 1106 58-59 0.47578505533287085 1106 60-61 0.3219066461410165 1106 62-63 0.30091532572633284 1106 64-65 0.4244009751249642 1106 66-67 0.5399128008412397 1106 68-69 0.5058091665668982 1106 70-71 0.5074413003598082 1106 72-73 0.545698222439583 1106 74-75 0.5055862877123047 1106 76-77 0.45453421248227244 1106 78-79 0.4684160676504341 1106 80-81 0.42136957990591384 1106 82-83 0.31833982956743156 1106 84-85 0.36727413868078074 1106 86-87 0.49711061367300147 1106 88-89 0.4686285831474528 1106 90-91 0.47683789131131604 1106 92-93 0.49286365351602157 1106 94-95 0.5258832368360444 1106 96-97 0.717575804899738 1106 98-99 0.8345084087656183 1106 100-101 1.0237053083697312 1107 1 0.1215879636061139 1107 2 0.10098583402028893 1107 3 0.24904632634154922 1107 4 0.41660738343316694 1107 5 0.2767775624264601 1107 6 0.25604655538939625 1107 7 0.3101554969200251 1107 8 0.22376630781600682 1107 9 0.20145550523137246 1107 10-11 0.22632316835363753 1107 12-13 0.21626771809042822 1107 14-15 0.38629702955669387 1107 16-17 0.324573867777012 1107 18-19 0.3653592032391444 1107 20-21 0.27033778560059574 1107 22-23 0.33955308013262453 1107 24-25 0.4702653507074501 1107 26-27 0.2894515614503774 1107 28-29 0.3234230012946284 1107 30-31 0.40670457280205596 1107 32-33 0.5722789918178677 1107 34-35 0.3574097202299171 1107 36-37 0.3799263911196462 1107 38-39 0.5167266693048518 1107 40-41 0.2825644011674413 1107 42-43 0.3287815922239332 1107 44-45 0.5562419086842283 1107 46-47 0.4384234511604248 1107 48-49 0.4560773898953059 1107 50-51 0.2365241848152877 1107 52-53 0.34042157398434014 1107 54-55 0.29766490123017064 1107 56-57 0.3135783823872593 1107 58-59 0.5485924313258295 1107 60-61 0.4861773463235828 1107 62-63 0.3653580908317764 1107 64-65 0.47325207942500214 1107 66-67 0.5662333373205719 1107 68-69 0.4529198929057898 1107 70-71 0.31628794832538176 1107 72-73 0.3608214349170602 1107 74-75 0.4536216953740002 1107 76-77 0.4584999785266497 1107 78-79 0.38509817314082184 1107 80-81 0.3660309057575155 1107 82-83 0.34809180821709873 1107 84-85 0.28556296441403006 1107 86-87 0.5416010056510814 1107 88-89 0.5884186991656506 1107 90-91 0.530933526058643 1107 92-93 0.5481112458754929 1107 94-95 0.5271384754123218 1107 96-97 0.6229429249394585 1107 98-99 0.7162916470342573 1107 100-101 0.7981752946016307 1108 1 0.15199536591972063 1108 2 0.07959354501831584 1108 3 0.07010242438705916 1108 4 0.3414249998392833 1108 5 0.13892842047746967 1108 6 0.2354965775474369 1108 7 0.25007771455782546 1108 8 0.1933783128894362 1108 9 0.2720282523699282 1108 10-11 0.18547955827402518 1108 12-13 0.024352270115976182 1108 14-15 0.23391649195679776 1108 16-17 0.1302355527049528 1108 18-19 0.21011217929317638 1108 20-21 0.21592848839249967 1108 22-23 0.17399599643635355 1108 24-25 0.36546125834662746 1108 26-27 0.28046907394292475 1108 28-29 0.20528878469595924 1108 30-31 0.24394054829068068 1108 32-33 0.44757040825148664 1108 34-35 0.33463171323102614 1108 36-37 0.37623287502068337 1108 38-39 0.3841862881865623 1108 40-41 0.3603930660308947 1108 42-43 0.3574875626696965 1108 44-45 0.3764876337105889 1108 46-47 0.4343918339291122 1108 48-49 0.5347235336322171 1108 50-51 0.4088090608066679 1108 52-53 0.36141769250693656 1108 54-55 0.2937911409343208 1108 56-57 0.18426207254145055 1108 58-59 0.2635622505467836 1108 60-61 0.23069407182366675 1108 62-63 0.14872771750700053 1108 64-65 0.13718627768597713 1108 66-67 0.2516875007399406 1108 68-69 0.381061368172837 1108 70-71 0.26051875869839947 1108 72-73 0.2381608655318317 1108 74-75 0.28605319646662153 1108 76-77 0.28766546088426637 1108 78-79 0.24733468347869447 1108 80-81 0.250067565853179 1108 82-83 0.2716906559990022 1108 84-85 0.29661715228364827 1108 86-87 0.35631646585065724 1108 88-89 0.30619594209417755 1108 90-91 0.2859585111592686 1108 92-93 0.3379238194172629 1108 94-95 0.4175821710678207 1108 96-97 0.4608836025170504 1108 98-99 0.4248590151175087 1108 100-101 0.6201143523500683 1109 1 -0.29345674664211785 1109 2 0.06223972132194078 1109 3 0.07782199295408532 1109 4 0.24463245350168705 1109 5 0.10744417571044096 1109 6 0.03185778101835979 1109 7 0.06853913518695265 1109 8 0.07532318534278204 1109 9 0.15084847962013015 1109 10-11 0.17831842869095027 1109 12-13 0.0925854944196729 1109 14-15 0.06612465746558627 1109 16-17 -0.01650928190338874 1109 18-19 0.12008893165172907 1109 20-21 0.2111310942909128 1109 22-23 0.13672579216348169 1109 24-25 0.14544643572253335 1109 26-27 0.17185307451961762 1109 28-29 -0.06042738683198223 1109 30-31 0.0031383695000997136 1109 32-33 0.2705279497683577 1109 34-35 0.17961472803563083 1109 36-37 0.26105072344245883 1109 38-39 0.2954596822521012 1109 40-41 0.2991622926413342 1109 42-43 0.23373811457590676 1109 44-45 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0.23565321167911435 2311 84-85 0.18179348994090105 2311 86-87 0.17435714866233454 2311 88-89 0.3444088106505596 2311 90-91 0.3627894156237623 2311 92-93 0.19838346579294353 2311 94-95 0.2105275674864977 2311 96-97 0.31800294627761616 2311 98-99 0.3939974339505845 2311 100-101 0.37176378857965275 2312 1 0.04956471445421684 2312 2 -0.20651589613133936 2312 3 0.08892956983618916 2312 4 0.22305939562917132 2312 5 0.19068359510127664 2312 6 0.08055511646656299 2312 7 0.08243317262374461 2312 8 0.10611641779912162 2312 9 0.10780343042392815 2312 10-11 0.028731875909720372 2312 12-13 0.03535366024605224 2312 14-15 0.11558907118969586 2312 16-17 -0.058647965226818144 2312 18-19 -0.022106432201574933 2312 20-21 0.02301192327755075 2312 22-23 0.07172042571574622 2312 24-25 0.060169962907878016 2312 26-27 0.007735437308490134 2312 28-29 -0.022689704395126853 2312 30-31 -0.03222066166409121 2312 32-33 -0.02330280105335447 2312 34-35 -0.027922064041241867 2312 36-37 0.06677818513703926 2312 38-39 0.07522708038016646 2312 40-41 0.027173741417470865 2312 42-43 0.0677581866936734 2312 44-45 0.13540983156040198 2312 46-47 0.1581826915671556 2312 48-49 0.16698921998637672 2312 50-51 -0.10515497634771975 2312 52-53 0.02885764159567472 2312 54-55 0.03263087879550852 2312 56-57 0.14029938940986497 2312 58-59 0.027910017763183248 2312 60-61 0.07939132135201632 2312 62-63 0.08644469966098 2312 64-65 0.1833637810484703 2312 66-67 0.17788454716486868 2312 68-69 0.050472639058636304 2312 70-71 0.08256694385650576 2312 72-73 0.10702660063081026 2312 74-75 0.14854301872856013 2312 76-77 0.1364090179924382 2312 78-79 0.1333186969828155 2312 80-81 0.03144501324939242 2312 82-83 -0.061394575222962544 2312 84-85 -0.04405069689626728 2312 86-87 -0.0777519664617401 2312 88-89 -0.020135062319933894 2312 90-91 0.034431444243463005 2312 92-93 -0.051501758976144174 2312 94-95 -0.041869855904316466 2312 96-97 0.01253957240358261 2312 98-99 -0.04588650983713549 2312 100-101 -0.02862893395353794 2313 1 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58-59 -0.1459154766710924 2313 60-61 -0.21719011110543818 2313 62-63 -0.2370303718274549 2313 64-65 -0.17803954156886448 2313 66-67 -0.2565704315297417 2313 68-69 -0.3630476557219531 2313 70-71 -0.19964251441380654 2313 72-73 -0.1728759863516025 2313 74-75 -0.2583925094821957 2313 76-77 -0.3744811707993385 2313 78-79 -0.23501747369378378 2313 80-81 -0.36174457769704205 2313 82-83 -0.34061401276385794 2313 84-85 -0.3428366961024736 2313 86-87 -0.43960920910404155 2313 88-89 -0.4446340330447107 2313 90-91 -0.31161383586712077 2313 92-93 -0.3253571340606456 2313 94-95 -0.26929100835200614 2313 96-97 -0.3078572166729714 2313 98-99 -0.20134632987153012 2313 100-101 -0.26724141481910735 2314 1 -0.6725427575670118 2314 2 -0.9658363918553228 2314 3 -0.6471872237617511 2314 4 -0.9555328485544194 2314 5 -0.8271682464665915 2314 6 -0.6318507680148997 2314 7 -0.5576986762628948 2314 8 -0.5889879547298733 2314 9 -0.5001027873327075 2314 10-11 -0.6749790489833316 2314 12-13 -0.6397013892711456 2314 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2315 36-37 -1.5169405813978045 2315 38-39 -1.581596707331535 2315 40-41 -2.546254870285914 2315 42-43 -2.0085596645695816 2315 44-45 -1.676616921778077 2315 46-47 -1.3717005468321446 2315 48-49 -1.781453133703387 2315 50-51 -1.5834345675296788 2315 52-53 -1.4946907428399108 2315 54-55 -1.4814951658420519 2315 56-57 -1.6015041994499875 2315 58-59 -1.7474525330507795 2315 60-61 -1.4291079216207123 2315 62-63 -1.3305501606119385 2315 64-65 -1.2446713716551159 2315 66-67 -1.2679616396707907 2315 68-69 -1.6157477829409501 2315 70-71 -1.3038815356356679 2315 72-73 -1.2553079283674293 2315 74-75 -1.3771975226450266 2315 76-77 -1.3615993728838767 2315 78-79 -1.4798106105209037 2315 80-81 -1.4790798687578324 2315 82-83 -1.3857569993625631 2315 84-85 -1.5149572005158696 2315 86-87 -1.5735126197069036 2315 88-89 -1.510796833342308 2315 90-91 -1.4436842743113338 2315 92-93 -1.5369470749569416 2315 94-95 -1.501783338330803 2315 96-97 -1.6386046575672353 2315 98-99 -1.6946232244971817 2315 100-101 -1.9249702686948176 2316 1 -0.9043961374587646 2316 2 -1.0313123521430043 2316 3 -1.4330372801599616 2316 4 -1.3310706948634774 2316 5 -0.8252438039365444 2316 6 -0.8679488500703414 2316 7 -1.0835587743235493 2316 8 -1.1202190369261373 2316 9 -1.3156979244634996 2316 10-11 -1.3415480332018035 2316 12-13 -1.231212584881611 2316 14-15 -1.2790846289860482 2316 16-17 -1.9786808587750002 2316 18-19 -1.3858744695886998 2316 20-21 -1.7183067968843133 2316 22-23 -1.2242244598312126 2316 24-25 -1.2689944268375086 2316 26-27 -1.3420880847487595 2316 28-29 -1.6476406647895914 2316 30-31 -1.4794649574799834 2316 32-33 -1.5812358502416828 2316 34-35 -2.0735301283716083 2316 36-37 -1.798234364015677 2316 38-39 -1.639872464247894 2316 40-41 -2.054430870795521 2316 42-43 -2.3196904167525503 2316 44-45 -2.476436712040787 2316 46-47 -2.3662185789410657 2316 48-49 -2.994911471095776 2316 50-51 -2.4365487328772275 2316 52-53 -3.1000400028596253 2316 54-55 -4.289071365093093 2316 56-57 -2.984174994971873 2316 58-59 -2.475267961054662 2316 60-61 -2.2680170943952476 2316 62-63 -2.955413039098108 2316 64-65 -2.8818122632096497 2316 66-67 -2.568594081355034 2316 68-69 -2.514729460135044 2316 70-71 -2.811818751556036 2316 72-73 -5.878367060974398 2316 74-75 -3.6248516048008597 2316 76-77 -2.959213934432821 2316 78-79 -2.940575008606448 2316 80-81 -2.7623102419834034 2316 82-83 -2.7434837476781944 2316 84-85 -2.815553375060471 2316 86-87 -2.694750277215462 2316 88-89 -2.4419577898940226 2316 90-91 -2.59674696893617 2316 92-93 -2.9185985336859943 2316 94-95 -3.0223032610587772 2316 96-97 -3.0934570764361524 2316 98-99 -3.520202699361441 2316 100-101 -3.6257751690261237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 120393.0 3 19682.0 4 16591.0 5 15650.0 6 18000.0 7 21440.0 8 26031.0 9 30344.0 10 34780.0 11 41027.0 12 42235.0 13 47840.0 14 54131.0 15 64420.0 16 81999.0 17 108917.0 18 157445.0 19 227910.0 20 293880.0 21 213652.0 22 129379.0 23 114421.0 24 120008.0 25 129484.0 26 143443.0 27 162258.0 28 186727.0 29 218436.0 30 256086.0 31 306550.0 32 370607.0 33 460564.0 34 590655.0 35 809727.0 36 1153431.0 37 1324587.0 38 1025585.0 39 169927.0 40 200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.861280319776148 46.678929627417126 24.689017803517686 17.77077224928904 2 17.19029953130228 47.97255335748071 18.978666469404764 15.858480641812239 3 42.96768448176419 21.008430920601302 19.56705188315076 16.456832714483745 4 16.935598197423367 19.733262576513436 20.68491668761205 42.64622253845115 5 16.81351070514919 45.81442616218157 21.43501518325536 15.937047949413877 6 42.80693540577272 20.75071015724054 19.617705988611892 16.824648448374845 7 17.413126051291293 20.139466741396483 46.66735417523812 15.780053032074104 8 42.41118086313397 20.797542173068933 20.47500238703312 16.316274576763977 9 42.0230146358152 20.534717363848063 21.08072604618603 16.36154195415071 10-11 16.465441926063576 33.41451346858434 33.465539548652494 16.654505056699588 12-13 16.306334892487993 20.858929606470454 33.46318912662308 29.37154637441847 14-15 16.444335114766776 46.29507143437718 20.54201191167863 16.718581539177414 16-17 29.30714345378611 20.666469863979316 20.619765060162603 29.40662162207197 18-19 16.62483801306344 33.376642718586766 20.60769979383163 29.390819474518164 20-21 29.481072943877606 33.396228696699254 20.62958423545668 16.49311412396646 22-23 29.385796813284255 20.5924281914179 33.47117715022705 16.5505978450708 24-25 16.569871032004603 33.19396037371887 21.05623329189082 29.179935302385708 26-27 16.79151463717778 25.55352383826685 40.8879091791203 16.767052345435076 28-29 26.892826020320204 20.496293301244723 35.96384485518209 16.647035823252978 30-31 24.871801733196722 21.536787604412968 36.37825979208431 17.213150870305995 32-33 19.79336019168292 23.726962215984184 37.64548966782151 18.83418792451139 34-35 19.152331135060244 22.819418252133737 38.35895895261092 19.6692916601951 36-37 19.00982689197147 22.908398457043933 38.55624256297776 19.525532088006837 38-39 19.463176050186746 22.919461628257494 38.22154212497856 19.395820196577198 40-41 19.352615373635594 22.976858552864048 38.120738672013154 19.549787401487208 42-43 19.6390409040078 23.188679094201568 38.07456058773921 19.097719414051422 44-45 19.434266620268794 23.198271634463588 38.06855151305881 19.29891023220881 46-47 19.470262408077048 23.06044539173554 37.982354511942276 19.48693768824513 48-49 19.53288788326888 23.071778390831973 37.9055643140697 19.489769411829442 50-51 19.730892423229445 23.309284605842638 37.25653625776522 19.7032867131627 52-53 20.005079200218344 23.8694090980341 36.28355048992089 19.84196121182667 54-55 20.67767159459069 24.6707635731433 34.743492655404154 19.908072176861857 56-57 21.018567853069317 25.40410102223279 33.40695451473834 20.170376609959547 58-59 21.157795868832725 26.04292349790356 32.551726383969196 20.247554249294517 60-61 21.36399625939015 26.239832077766252 32.19616909400918 20.200002568834417 62-63 21.13889240165667 26.20021242854802 32.2469771282182 20.413918041577116 64-65 20.81182537102075 25.988493855955547 32.42934953472937 20.77033123829434 66-67 20.8826682298206 25.637352801392392 32.90681930202769 20.573159666759313 68-69 20.788487012690403 25.59425640364241 33.084625078257034 20.532631505410148 70-71 20.4693496628385 25.46268274895052 33.634217794452674 20.433749793758306 72-73 20.343805174488413 25.391409728464676 33.483136032479514 20.781649064567397 74-75 20.741272498208946 25.49782055969233 32.905348784880545 20.85555815721818 76-77 20.935126807698996 25.727554830108097 32.66506803344952 20.672250328743385 78-79 20.992651209786306 25.863040441642145 32.227843289221106 20.916465059350443 80-81 20.698262324446425 26.181247394325002 32.187001133142836 20.933489148085744 82-83 21.290464311936816 26.35386272435809 31.584710694615264 20.770962269089825 84-85 21.05641452301274 26.73338294161261 31.233477602893693 20.976724932480952 86-87 21.109135384796122 26.6723428715825 31.28399871815149 20.934523025469886 88-89 21.039376159997644 26.498223374504015 31.135956963328354 21.326443502169987 90-91 21.193292756228534 26.538573336853617 31.094251782223246 21.173882124694607 92-93 21.181618543441804 26.790836011323922 31.030164701123137 20.997380744111137 94-95 21.26054785203859 26.607617754612296 30.746069758983623 21.385764634365493 96-97 20.963661666093536 26.586361975584595 31.132968320151306 21.317008038170567 98-99 21.228230059726112 26.60024879164103 30.707041125849344 21.464480022783512 100-101 21.59610299925473 26.403895066515215 30.466508887222943 21.53349304700711 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13310.0 1 7947.5 2 1936.5 3 1148.5 4 994.5 5 937.5 6 1024.0 7 1218.0 8 1144.0 9 1165.5 10 1623.0 11 9248.5 12 153305.0 13 172396.0 14 48402.5 15 43613.5 16 47187.0 17 50977.5 18 54105.5 19 56730.0 20 59117.5 21 61738.5 22 65402.5 23 69809.5 24 75204.0 25 81998.0 26 90537.5 27 100495.0 28 110209.5 29 119764.0 30 130740.0 31 143023.5 32 155164.0 33 168924.5 34 187661.5 35 202857.5 36 213601.5 37 225110.0 38 234178.0 39 241714.5 40 252520.0 41 261997.5 42 266179.0 43 274059.0 44 286747.0 45 295843.5 46 297667.0 47 300061.5 48 301273.5 49 301456.0 50 305337.0 51 302230.5 52 293922.0 53 290121.5 54 275636.0 55 251306.0 56 235477.0 57 221109.0 58 208944.5 59 175369.0 60 128679.5 61 99118.5 62 75038.0 63 56153.0 64 40834.0 65 28372.5 66 18695.0 67 11936.5 68 8017.5 69 5568.0 70 4207.0 71 3411.5 72 2991.0 73 2786.0 74 2624.5 75 2431.5 76 2299.5 77 2191.5 78 2080.0 79 1943.0 80 1758.0 81 1629.0 82 1528.5 83 1402.0 84 1241.5 85 1056.5 86 917.0 87 795.5 88 655.0 89 517.5 90 382.5 91 272.0 92 212.5 93 152.5 94 101.5 95 78.5 96 43.0 97 26.5 98 16.0 99 6.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10138109041233753 2 0.083558558994083 3 0.09013323604530167 4 0.19774522954539545 5 0.0760277606069845 6 0.09390400670703003 7 0.09844826878654882 8 0.0880276205190944 9 0.08047533625927948 10-11 0.0785953223965944 12-13 0.07212270324077864 14-15 0.0786168082693108 16-17 0.06876553562884101 18-19 0.06025175856496716 20-21 0.05542818014013516 22-23 0.045034389213576234 24-25 0.04715611914432082 26-27 0.046473942685575094 28-29 0.04518479032259104 30-31 0.03570952045465826 32-33 0.03975423599352072 34-35 0.05293044743685355 36-37 0.04816058369381256 38-39 0.052323471532615226 40-41 0.049820367361154534 42-43 0.047822181198529254 44-45 0.052151584550884034 46-47 0.04566285099053096 48-49 0.04130659029728068 50-51 0.045200904727128344 52-53 0.04111858891101217 54-55 0.04496993159542703 56-57 0.043557235464323676 58-59 0.05052940116079577 60-61 0.04954105101584132 62-63 0.05455800229512092 64-65 0.05067443080163146 66-67 0.04486250223184503 68-69 0.044765815804621224 70-71 0.0541121704362556 72-73 0.04157516370623569 74-75 0.0490146471342895 76-77 0.04783292413488745 78-79 0.04573805154503836 80-81 0.04545336373154605 82-83 0.04455632854563631 84-85 0.04528684821799395 86-87 0.050389742988139155 88-89 0.04302546011459275 90-91 0.045791766226829365 92-93 0.04180076536975791 94-95 0.04488398810456143 96-97 0.030531425130005646 98-99 0.03724576035388092 100-101 0.025928076900516756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9308442.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.09804833595151 #Duplication Level Percentage of deduplicated Percentage of total 1 91.29672907961445 52.12865049902096 2 5.298162649255179 6.050294940778102 3 1.335935893605521 2.2883799668046185 4 0.5903057117646775 1.3482121625331127 5 0.3283860702945732 0.9375101857266356 6 0.22246985839510852 0.7621556836761717 7 0.1695121351336139 0.6775168459772603 8 0.12648718733545294 0.5777737229086597 9 0.10765663032227266 0.5532285133644109 >10 0.5001046526756631 4.334122245969543 >50 0.010587778354831675 0.4466119408379522 >100 0.008568573385068966 1.0188039956960124 >500 0.0023770972859235265 0.9457844939687092 >1k 0.0023770972555228814 2.7100926582993665 >5k 1.8865851234308576E-4 0.7857551647030794 >10k+ 1.509268098744686E-4 24.43510697973555 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1086356 11.670653370349195 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 26485 0.2845266694469386 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10942 0.11754920963142917 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 10409 0.11182322455250836 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.1485872716400877E-5 0.0 2 0.0 0.0 0.0 2.1485872716400877E-5 0.0 3 0.0 0.0 0.0 2.1485872716400877E-5 0.0 4 0.0 0.0 0.0 2.1485872716400877E-5 0.0 5 0.0 0.0 0.0 2.1485872716400877E-5 0.0 6 0.0 0.0 0.0 2.1485872716400877E-5 0.0 7 0.0 0.0 0.0 2.1485872716400877E-5 0.0 8 0.0 0.0 0.0 2.1485872716400877E-5 0.0 9 0.0 0.0 0.0 2.1485872716400877E-5 0.0 10-11 0.0 0.0 0.0 2.6857340895501095E-5 0.0 12-13 0.0 0.0 0.0 3.222880907460131E-5 0.0 14-15 5.371468179100219E-6 5.371468179100219E-6 0.0 6.982908632830284E-5 0.0 16-17 1.611440453730066E-5 1.0742936358200439E-5 0.0 7.520055450740306E-5 0.0 18-19 2.1485872716400877E-5 1.0742936358200439E-5 0.0 9.668642722380394E-5 0.0 20-21 2.1485872716400877E-5 1.0742936358200439E-5 0.0 1.0742936358200438E-4 0.0 22-23 2.1485872716400877E-5 1.0742936358200439E-5 0.0 1.128008317611046E-4 0.0 24-25 2.1485872716400877E-5 1.0742936358200439E-5 0.0 1.2891523629840525E-4 0.0 26-27 2.1485872716400877E-5 1.0742936358200439E-5 0.0 1.2891523629840525E-4 0.0 28-29 3.222880907460131E-5 1.0742936358200439E-5 0.0 1.396581726566057E-4 0.0 30-31 3.222880907460131E-5 1.0742936358200439E-5 0.0 1.5040110901480612E-4 0.0 32-33 3.760027725370153E-5 1.0742936358200439E-5 0.0 1.5040110901480612E-4 0.0 34-35 8.057202268650328E-5 1.0742936358200439E-5 0.0 1.6114404537300657E-4 0.0 36-37 1.128008317611046E-4 1.0742936358200439E-5 0.0 1.7188698173120702E-4 0.0 38-39 1.1817229994020481E-4 1.0742936358200439E-5 0.0 1.7188698173120702E-4 0.0 40-41 1.6114404537300657E-4 1.0742936358200439E-5 0.0 1.7725844991030721E-4 0.0 42-43 1.8800138626850766E-4 1.0742936358200439E-5 0.0 1.8262991808940744E-4 1.611440453730066E-5 44-45 1.933728544476079E-4 1.0742936358200439E-5 0.0 1.8262991808940744E-4 2.6857340895501095E-5 46-47 2.1485872716400876E-4 1.0742936358200439E-5 0.0 1.933728544476079E-4 3.222880907460131E-5 48-49 2.6857340895501095E-4 1.0742936358200439E-5 0.0 2.041157908058083E-4 3.222880907460131E-5 50-51 3.061736862087125E-4 1.0742936358200439E-5 0.0 2.1485872716400876E-4 3.222880907460131E-5 52-53 4.189745179698171E-4 1.0742936358200439E-5 0.0 2.256016635222092E-4 3.222880907460131E-5 54-55 5.210324133727213E-4 1.0742936358200439E-5 0.0 2.256016635222092E-4 3.222880907460131E-5 56-57 6.12347372417425E-4 1.0742936358200439E-5 0.0 2.309731317013094E-4 3.760027725370153E-5 58-59 7.036623314621287E-4 1.611440453730066E-5 0.0 2.3634459988040963E-4 4.2971745432801754E-5 60-61 8.325775677605339E-4 2.1485872716400877E-5 0.0 2.4708753623861005E-4 4.2971745432801754E-5 62-63 9.776072085962398E-4 2.1485872716400877E-5 0.0 2.4708753623861005E-4 4.2971745432801754E-5 64-65 0.0011494941903274468 2.1485872716400877E-5 0.0 2.578304725968105E-4 4.2971745432801754E-5 66-67 0.0013106382357004533 2.1485872716400877E-5 0.0 2.793163453132114E-4 4.834321361190197E-5 68-69 0.0015416113674017627 2.1485872716400877E-5 0.0 2.793163453132114E-4 6.982908632830284E-5 70-71 0.0018209277127149742 2.1485872716400877E-5 0.0 2.9005928167141184E-4 7.520055450740306E-5 72-73 0.002100244058028186 2.1485872716400877E-5 0.0 2.9005928167141184E-4 7.520055450740306E-5 74-75 0.0024440180214905997 2.1485872716400877E-5 0.0 2.9005928167141184E-4 8.594349086560351E-5 76-77 0.0029059642848932184 2.1485872716400877E-5 0.0 2.9005928167141184E-4 8.594349086560351E-5 78-79 0.0034431111028032403 2.1485872716400877E-5 0.0 2.9543074985051204E-4 8.594349086560351E-5 80-81 0.004093058752474367 2.1485872716400877E-5 0.0 3.115451543878127E-4 8.594349086560351E-5 82-83 0.004952493661130402 2.1485872716400877E-5 0.0 3.115451543878127E-4 8.594349086560351E-5 84-85 0.005999929956054945 2.1485872716400877E-5 0.0 3.1691662256691294E-4 9.131495904470373E-5 86-87 0.0076865709642924135 2.1485872716400877E-5 0.0 3.330310271042136E-4 9.668642722380394E-5 88-89 0.009754586213245996 2.1485872716400877E-5 0.0 3.3840249528331384E-4 9.668642722380394E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCAGT 241395 0.0 91.332794 1 AGTGGTA 240560 0.0 91.17682 5 GTATCAA 241370 0.0 91.01823 9 GGTATCA 241510 0.0 90.9792 8 TGGTATC 241605 0.0 90.86372 7 GTGGTAT 242065 0.0 90.74109 6 AGCAGTG 244375 0.0 90.165504 2 GCAGTGG 244160 0.0 90.119194 3 CAGTGGT 243880 0.0 89.85729 4 TCAACGC 240840 0.0 46.17779 12-13 CAACGCA 241110 0.0 46.03544 12-13 AACGCAG 242105 0.0 46.0248 14-15 ATCAACG 241080 0.0 45.998432 10-11 CGCAGAG 242870 0.0 45.88979 16-17 ACGCAGA 242195 0.0 45.854694 14-15 TATCAAC 242300 0.0 45.77565 10-11 AGAGTAC 236420 0.0 45.556816 18-19 CAGAGTA 242280 0.0 45.42832 18-19 GAGTACT 140140 0.0 45.19369 20-21 GCAGAGT 244970 0.0 45.135735 16-17 >>END_MODULE