FastQCFastQC Report
Sat 18 Jun 2016
SRR578553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9308442
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190110.2042339631057485No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT104610.11238185724313478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG3650.029.9302583
CAACGCA67750.019.34922812-13
ATCAACG69700.019.11454410-11
CGCGTAG3000.018.20816254-55
GGTATCA87200.018.0292518
CTCGCGT3100.017.621752-53
ACTCGCG3250.017.53970750-51
GTATCAA90900.017.399899
AGTGGTA90400.017.3911425
CGTACGC3300.017.27172920-21
TCAACGC65650.017.0742310-11
TCGCGTA3400.016.76540452-53
ACGTACG3350.016.30502920-21
AAGCAGT102650.016.2178751
TGGTATC98250.016.098227
ACGCAGA83150.015.96553314-15
GTGGTAT102350.015.8245996
CGTAGCG3950.015.63200856-57
AACGCAG73450.015.19637212-13
CGCTAGT4350.014.74108190-91
Produced by FastQC (version 0.11.5)