FastQCFastQC Report
Sat 18 Jun 2016
SRR578552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9362647
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110015411.750459031511069No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT268820.2871196575071131No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111350.11893004189947566No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG105360.11253227853191518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2446150.091.273041
GGTATCA2440350.091.2382358
AGTGGTA2434650.091.21425
GTATCAA2444200.091.202899
TGGTATC2440450.091.060727
GTGGTAT2450450.090.822436
AGCAGTG2474750.090.0713042
GCAGTGG2474800.089.966743
CAGTGGT2474550.089.74494
GTATCGA7200.050.8160749
TCAACGC2435250.046.28151712-13
CAACGCA2434200.046.1853512-13
AACGCAG2444800.046.1282914-15
ATCAACG2438350.046.1212710-11
ACGCAGA2448700.045.99079514-15
CGCAGAG2455450.045.9669816-17
TATCAAC2454850.045.84707610-11
AGAGTAC2391100.045.57928518-19
CAGAGTA2452400.045.43561618-19
GAGTACT1421650.045.29731420-21
Produced by FastQC (version 0.11.5)