FastQCFastQC Report
Sat 18 Jun 2016
SRR578552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9362647
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188990.20185530865363183No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107100.11439072732315979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4900.033.9112633
CGCGTAG3300.025.89760254-55
CTCGCGT4000.022.55466352-53
CGTACGC3800.021.86360520-21
ACTCGCG3950.021.63868750-51
TCGCGTA4200.020.91535252-53
CGTAGCG4200.020.91216956-57
CAACGCA66350.019.64112912-13
ACGTACG4200.019.21617520-21
ATCAACG69200.019.03802710-11
CGCTAGT4700.018.18288890-91
AGTGGTA87200.017.9666525
CGACGAC4800.017.80350760-61
TCAACGC63900.017.68239810-11
GGTATCA88100.017.567378
GTATCAA89800.017.3934049
AAGCAGT98450.016.710941
ACGCAGA80350.016.48478914-15
TGGTATC100400.015.7461947
GTGGTAT100950.015.6604036
Produced by FastQC (version 0.11.5)