##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578552_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9362647 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.394888112304137 31.0 31.0 34.0 30.0 34.0 2 31.768253358265028 31.0 31.0 34.0 30.0 34.0 3 31.845244619390222 31.0 31.0 34.0 30.0 34.0 4 35.546155056363865 37.0 35.0 37.0 33.0 37.0 5 35.369807384599675 37.0 35.0 37.0 33.0 37.0 6 35.36618907024904 37.0 35.0 37.0 32.0 37.0 7 35.358337551335644 37.0 35.0 37.0 32.0 37.0 8 35.31687555880298 37.0 35.0 37.0 32.0 37.0 9 36.85700304625391 39.0 37.0 39.0 32.0 39.0 10-11 36.85063342663672 39.0 37.0 39.0 32.0 39.0 12-13 36.84115742054571 39.0 37.0 39.0 32.0 39.0 14-15 38.01320043359533 40.0 37.0 41.0 33.0 41.0 16-17 37.94441000499111 40.0 37.0 41.0 33.0 41.0 18-19 37.87550636054098 40.0 37.0 41.0 32.5 41.0 20-21 37.831989233386665 40.0 37.0 41.0 32.0 41.0 22-23 37.62905084427513 39.5 36.5 41.0 32.0 41.0 24-25 37.63188823630753 39.0 36.0 41.0 32.0 41.0 26-27 37.460846969879356 39.0 36.0 41.0 32.0 41.0 28-29 37.311023527854886 39.0 36.0 41.0 31.5 41.0 30-31 37.169012353023675 39.0 36.0 41.0 31.0 41.0 32-33 37.03042590412733 39.0 36.0 40.5 31.0 41.0 34-35 36.881698466256395 39.0 35.5 40.0 30.0 41.0 36-37 36.73062799441226 39.0 35.0 40.0 30.0 41.0 38-39 36.55756449004218 38.0 35.0 40.0 30.0 41.0 40-41 36.39614881347123 38.0 35.0 40.0 30.0 41.0 42-43 36.47943535626196 38.0 35.0 40.0 30.0 41.0 44-45 36.41458313017675 38.0 35.0 40.0 30.0 41.0 46-47 36.26234658852353 38.0 35.0 40.0 30.0 41.0 48-49 36.11385845263631 38.0 35.0 40.0 29.0 41.0 50-51 35.92423430040671 38.0 34.5 40.0 28.5 41.0 52-53 35.96690070660573 38.0 35.0 40.0 28.0 41.0 54-55 35.95846206740465 38.0 35.0 40.0 28.0 41.0 56-57 35.79722043349493 38.0 34.0 40.0 28.0 41.0 58-59 35.56896329638403 38.0 34.0 40.0 28.0 41.0 60-61 35.310360627715646 38.0 34.0 40.0 28.0 41.0 62-63 35.02982756906247 37.0 34.0 40.0 27.5 41.0 64-65 34.70676604596969 37.0 33.0 40.0 26.5 41.0 66-67 34.36470498140109 36.0 33.0 39.0 26.0 41.0 68-69 33.98331331940636 36.0 32.5 39.0 26.0 41.0 70-71 33.58696894158244 35.5 32.0 39.0 26.0 40.0 72-73 33.18952818577908 35.0 32.0 38.0 26.0 40.0 74-75 32.773381875873355 35.0 31.5 37.0 25.0 39.5 76-77 31.393012948154514 34.0 29.5 36.0 23.0 39.0 78-79 32.04646308891064 34.5 31.0 36.0 24.5 39.0 80-81 31.91827177720147 35.0 31.0 36.0 24.0 38.5 82-83 31.54750916060383 34.0 31.0 35.5 23.0 37.0 84-85 31.262807676077074 34.0 31.0 35.0 23.0 37.0 86-87 30.94290941439958 34.0 31.0 35.0 21.5 36.5 88-89 30.62829203108907 34.0 30.5 35.0 20.0 36.0 90-91 30.282009724386704 34.0 30.0 35.0 19.5 36.0 92-93 29.90600323818681 34.0 30.0 35.0 17.5 35.5 94-95 29.520153648855928 34.0 29.5 35.0 10.0 35.0 96-97 29.050670713100686 34.0 29.0 35.0 2.0 35.0 98-99 28.23078996783709 34.0 29.0 35.0 2.0 35.0 100-101 27.17080815927376 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3460479830181562 1101 2 0.25007892556051914 1101 3 0.3460239520657318 1101 4 0.2991251879619057 1101 5 0.2535136358506378 1101 6 0.1350985762764907 1101 7 0.18355884763728625 1101 8 0.196086376216428 1101 9 0.2504853052330276 1101 10-11 0.3158875532552443 1101 12-13 0.32303811823628337 1101 14-15 0.22215514585139573 1101 16-17 0.2812373323992361 1101 18-19 0.27985913265862195 1101 20-21 0.2972760706241715 1101 22-23 0.44166999112351135 1101 24-25 0.39567024248036375 1101 26-27 0.3167248276273895 1101 28-29 0.2695857530038879 1101 30-31 0.3529726154719057 1101 32-33 0.4000134064695473 1101 34-35 0.3984441042487816 1101 36-37 0.4142700838335571 1101 38-39 0.24561634567333357 1101 40-41 0.27762683262624677 1101 42-43 0.3535878420635896 1101 44-45 0.35554780227892024 1101 46-47 0.2612336114322318 1101 48-49 0.21967867543555286 1101 50-51 0.25701511150057854 1101 52-53 0.24584099312941277 1101 54-55 0.38763452828778355 1101 56-57 0.45448180045804776 1101 58-59 0.44352775710853365 1101 60-61 0.39383248747557076 1101 62-63 0.25399322192451734 1101 64-65 0.24272279157366938 1101 66-67 0.31404796274537006 1101 68-69 0.21965519146787926 1101 70-71 0.22726406388268572 1101 72-73 0.38525789550475764 1101 74-75 0.41515039327858005 1101 76-77 0.2996838757250515 1101 78-79 0.33984373949372326 1101 80-81 0.3427173354583566 1101 82-83 0.399499820971009 1101 84-85 0.35313747168903475 1101 86-87 0.40781597482578036 1101 88-89 0.5131380057358825 1101 90-91 0.43085255447557813 1101 92-93 0.5054156458454564 1101 94-95 0.4155761597151084 1101 96-97 0.34048431004919877 1101 98-99 0.6976640323568368 1101 100-101 0.844935375211989 1102 1 0.3114954486983237 1102 2 0.31469854584896595 1102 3 0.3650856869310424 1102 4 0.3311834600798278 1102 5 0.2837814767650002 1102 6 0.20676453164371367 1102 7 0.25863680210130013 1102 8 0.09325620463722828 1102 9 0.23297451278467918 1102 10-11 0.27869907669350624 1102 12-13 0.3436454319253244 1102 14-15 0.3618071387591115 1102 16-17 0.23087228160519402 1102 18-19 0.32145187306475975 1102 20-21 0.3162695747851103 1102 22-23 0.4582665141675335 1102 24-25 0.36453848035629477 1102 26-27 0.43694317701444874 1102 28-29 0.35375031862061945 1102 30-31 0.4155910124436062 1102 32-33 0.4476418244309315 1102 34-35 0.3903569015700086 1102 36-37 0.38556181347316 1102 38-39 0.4038100484391407 1102 40-41 0.42264691258564824 1102 42-43 0.33616862499675193 1102 44-45 0.38595956724471137 1102 46-47 0.3208825666326831 1102 48-49 0.3510770957002123 1102 50-51 0.4198104873340469 1102 52-53 0.43954121340319574 1102 54-55 0.4407773637211321 1102 56-57 0.4905250309962881 1102 58-59 0.48929826696427625 1102 60-61 0.483284390523103 1102 62-63 0.3220829014816573 1102 64-65 0.3571362486748413 1102 66-67 0.4471914585095291 1102 68-69 0.4295074349696364 1102 70-71 0.4574158377992461 1102 72-73 0.4816571303977426 1102 74-75 0.34079390878655147 1102 76-77 0.3012631437127524 1102 78-79 0.331307311238362 1102 80-81 0.46352854454945813 1102 82-83 0.480901022766119 1102 84-85 0.45142883151322977 1102 86-87 0.4855636824637237 1102 88-89 0.4769330788075621 1102 90-91 0.5189076502793846 1102 92-93 0.502120631065889 1102 94-95 0.5513940474365349 1102 96-97 0.7310044193663039 1102 98-99 1.149312949936757 1102 100-101 1.2885539847907275 1103 1 0.19244736436560217 1103 2 0.24131232707617656 1103 3 0.24268611996847866 1103 4 0.23443711481485963 1103 5 0.225021268182914 1103 6 0.06666095736441946 1103 7 0.20786700265415448 1103 8 0.019750226018736328 1103 9 0.17021686967115812 1103 10-11 0.11158952102670128 1103 12-13 0.15564256654079145 1103 14-15 0.3002104216927677 1103 16-17 0.2289210574650653 1103 18-19 0.21802289890355553 1103 20-21 0.3208002146942093 1103 22-23 0.27960931425963054 1103 24-25 0.32127414948438826 1103 26-27 0.2045658785968385 1103 28-29 0.2295716546343627 1103 30-31 0.3362667400950343 1103 32-33 0.17096586068703346 1103 34-35 0.3122822431482817 1103 36-37 0.3582851780119185 1103 38-39 0.3768304237503557 1103 40-41 0.25016734268702834 1103 42-43 0.34994910434843973 1103 44-45 0.22820464797825935 1103 46-47 0.3109609462911678 1103 48-49 0.2754998550596568 1103 50-51 0.1848660294379485 1103 52-53 0.16356477743804732 1103 54-55 0.2889418582553773 1103 56-57 0.20211869079906108 1103 58-59 0.16505845997634339 1103 60-61 0.2133787165233585 1103 62-63 0.3228726208053416 1103 64-65 0.18621830608294232 1103 66-67 0.17358036177308378 1103 68-69 0.30456287766139667 1103 70-71 0.2708063197184032 1103 72-73 0.15493608847143037 1103 74-75 0.22245275140053167 1103 76-77 0.18084432947163265 1103 78-79 0.3727204485927089 1103 80-81 0.2644030955606844 1103 82-83 0.460479557534768 1103 84-85 0.20549680213117583 1103 86-87 0.21087559824721325 1103 88-89 0.3173773416470702 1103 90-91 0.35386508962981367 1103 92-93 0.3022901332934502 1103 94-95 0.33523659332408684 1103 96-97 0.3989142370362764 1103 98-99 0.9126212613721805 1103 100-101 0.7566154252571096 1104 1 0.23334821846458453 1104 2 0.1961090195487003 1104 3 0.11142081574161722 1104 4 0.15548890509619184 1104 5 0.15113467498805733 1104 6 0.12800497521539 1104 7 0.1574652910000438 1104 8 0.11011581019306504 1104 9 0.2247989249521396 1104 10-11 0.09749734681124522 1104 12-13 0.13724055139424962 1104 14-15 0.24954295975352636 1104 16-17 0.2681176989594931 1104 18-19 0.1235126773648858 1104 20-21 0.20208565094567632 1104 22-23 0.30031633281911496 1104 24-25 0.23524567735816504 1104 26-27 0.32889874067087277 1104 28-29 0.2963989760814485 1104 30-31 0.1988751495354819 1104 32-33 0.24544174761935977 1104 34-35 0.2254125569659351 1104 36-37 0.19544668134709298 1104 38-39 0.22867054912189388 1104 40-41 0.31870290530367384 1104 42-43 0.16776628634021762 1104 44-45 0.1545183111859103 1104 46-47 0.18940150821658364 1104 48-49 0.18309118900069166 1104 50-51 0.15886136688696695 1104 52-53 0.19226354013936486 1104 54-55 0.21374623524265957 1104 56-57 0.20749202429080782 1104 58-59 0.15198072811244145 1104 60-61 0.23433663475969269 1104 62-63 0.16675619527655527 1104 64-65 0.19153737275191673 1104 66-67 0.24957308443500636 1104 68-69 0.32294763370100554 1104 70-71 0.22223198351359486 1104 72-73 0.30831146432682743 1104 74-75 0.300236543824667 1104 76-77 0.15453603948407135 1104 78-79 0.23931814183421807 1104 80-81 0.3494354914876219 1104 82-83 0.42864783490740876 1104 84-85 0.3198944668595267 1104 86-87 0.3573035139346388 1104 88-89 0.3432505250126603 1104 90-91 0.3837947463485527 1104 92-93 0.523662426702586 1104 94-95 0.4860078961166501 1104 96-97 0.46567263546027604 1104 98-99 0.8130185664457983 1104 100-101 0.949321913514872 1105 1 0.24505727375442277 1105 2 0.15743222188708472 1105 3 0.102494851889535 1105 4 0.14290541035030202 1105 5 0.16053169733282147 1105 6 0.05579038802881797 1105 7 0.17016433173218815 1105 8 0.06586818950558637 1105 9 0.16589847932603874 1105 10-11 0.06947864159526063 1105 12-13 0.11724654405480806 1105 14-15 0.12213757109281431 1105 16-17 0.10464117034337761 1105 18-19 0.14330101472150147 1105 20-21 0.04331690860068704 1105 22-23 0.274206180747548 1105 24-25 0.19958983606171898 1105 26-27 0.21529176504752456 1105 28-29 0.2156807931684881 1105 30-31 0.19224423422161152 1105 32-33 0.23013875022268593 1105 34-35 0.15325031192386263 1105 36-37 0.21828180281161735 1105 38-39 0.18380848825529483 1105 40-41 0.25128605151808614 1105 42-43 0.22706386017101465 1105 44-45 0.18267029587757122 1105 46-47 0.20184064850908356 1105 48-49 0.16019207299615346 1105 50-51 0.23743146189821118 1105 52-53 0.31926462708938885 1105 54-55 0.33267469343565637 1105 56-57 0.2706977179425607 1105 58-59 0.23239574066347757 1105 60-61 0.11634095082779794 1105 62-63 0.13776154991642642 1105 64-65 0.1713845391000106 1105 66-67 0.21528851160844198 1105 68-69 0.18150053102493757 1105 70-71 0.23135443588507343 1105 72-73 0.2790590045139325 1105 74-75 0.2634898874509588 1105 76-77 0.16738103167202567 1105 78-79 0.25772016664601693 1105 80-81 0.32519041976166463 1105 82-83 0.4008168581063387 1105 84-85 0.32670232692049694 1105 86-87 0.31053199478218474 1105 88-89 0.25087186273367834 1105 90-91 0.4774321326434112 1105 92-93 0.6105939270031264 1105 94-95 0.5637919174051618 1105 96-97 0.6187006326813922 1105 98-99 1.078506730290961 1105 100-101 1.1359091479514447 1106 1 0.16599920285866432 1106 2 0.13590946049743025 1106 3 0.10644383469756846 1106 4 0.06858756776935593 1106 5 0.04120897911419519 1106 6 -0.03983623479762599 1106 7 0.049135951691212654 1106 8 0.013079002505996584 1106 9 0.09101628097637615 1106 10-11 -0.011893323547724322 1106 12-13 0.005889365271656288 1106 14-15 -0.002197492368992471 1106 16-17 0.004851824357650969 1106 18-19 0.12541128443238847 1106 20-21 0.11924761350979196 1106 22-23 0.23855461784764742 1106 24-25 0.16192071646906925 1106 26-27 0.10936834527560535 1106 28-29 0.15114970103507375 1106 30-31 0.19803350826259702 1106 32-33 0.18530447112808446 1106 34-35 0.1740342630805074 1106 36-37 0.12397801772083028 1106 38-39 0.11177624675449493 1106 40-41 0.18199833621336836 1106 42-43 0.15308527909832037 1106 44-45 0.06700788117400691 1106 46-47 0.05671324801576105 1106 48-49 0.11289156149548063 1106 50-51 0.16583075270236236 1106 52-53 0.11970845972440713 1106 54-55 0.12697698770423216 1106 56-57 0.09286614353863598 1106 58-59 0.08604146887892483 1106 60-61 0.24313010235753296 1106 62-63 0.1845892645356244 1106 64-65 0.07730911991252754 1106 66-67 0.14096576418058504 1106 68-69 0.22404897871574292 1106 70-71 0.2753764576740707 1106 72-73 0.29406878876746134 1106 74-75 0.35645228328263556 1106 76-77 0.17516198763705404 1106 78-79 0.36830053179318867 1106 80-81 0.45441019775436686 1106 82-83 0.6443254947513282 1106 84-85 0.557471155025997 1106 86-87 0.6290587573753008 1106 88-89 0.6221546352508973 1106 90-91 0.6598672801503191 1106 92-93 0.6635127600469737 1106 94-95 0.6770397393128782 1106 96-97 0.7667681355611151 1106 98-99 1.278491875462187 1106 100-101 1.2753658230356066 1107 1 0.21566403197338602 1107 2 0.10469837670101612 1107 3 0.05377948089718032 1107 4 0.01454055957637479 1107 5 0.04243918963320681 1107 6 -0.0021983355174342023 1107 7 -0.016324056143126597 1107 8 0.059667004356896314 1107 9 0.05487466080666792 1107 10-11 0.006087824779136497 1107 12-13 0.07329573409761281 1107 14-15 0.013244064825549628 1107 16-17 0.016459465343992008 1107 18-19 -0.00487794093955074 1107 20-21 0.006538849336287456 1107 22-23 0.13418281292646128 1107 24-25 0.17595279208693881 1107 26-27 0.04583647441305061 1107 28-29 -0.0014276999548883396 1107 30-31 0.011773208491426601 1107 32-33 -0.006037004323822259 1107 34-35 0.07588217869328417 1107 36-37 0.12141241775420752 1107 38-39 0.10289517933095027 1107 40-41 0.1609453966212584 1107 42-43 0.08825193833688161 1107 44-45 0.19431789372120534 1107 46-47 0.1128601522069701 1107 48-49 0.06387285099816609 1107 50-51 0.10663371203978045 1107 52-53 0.07865909107496094 1107 54-55 0.022832348285511728 1107 56-57 0.12490764251703723 1107 58-59 0.03202850613465813 1107 60-61 0.11783905311478549 1107 62-63 0.11740624224654539 1107 64-65 0.19275065822208148 1107 66-67 0.14918150275048703 1107 68-69 0.19318758207050735 1107 70-71 0.17594443228189505 1107 72-73 0.1252612246808127 1107 74-75 0.17558498192906313 1107 76-77 0.07666829467970615 1107 78-79 0.14495628209359523 1107 80-81 0.1285178146098893 1107 82-83 0.2282660121380644 1107 84-85 0.27008537042455316 1107 86-87 0.3526233529959448 1107 88-89 0.2993804270168887 1107 90-91 0.4326911496252954 1107 92-93 0.39111274329388834 1107 94-95 0.40922289043959026 1107 96-97 0.4096887574800796 1107 98-99 0.9648341990601779 1107 100-101 1.0387055222908046 1108 1 0.07419172937057894 1108 2 -0.0046524254254691755 1108 3 -0.07821920738802035 1108 4 -0.04580624133573252 1108 5 -0.014849186786101143 1108 6 0.022434625227724325 1108 7 3.685857376396484E-4 1108 8 0.020748729146134792 1108 9 -0.02889220376079038 1108 10-11 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2316 92-93 -1.3171725010255209 2316 94-95 -1.5220968961508312 2316 96-97 -1.6626612954960471 2316 98-99 -1.249129459576416 2316 100-101 -1.6279597168921853 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 151.0 3 109.0 4 276.0 5 667.0 6 1245.0 7 2289.0 8 4060.0 9 6414.0 10 8879.0 11 11081.0 12 13925.0 13 16601.0 14 19288.0 15 22394.0 16 25830.0 17 29855.0 18 35338.0 19 39721.0 20 42985.0 21 45831.0 22 51234.0 23 60697.0 24 74660.0 25 94998.0 26 120791.0 27 152349.0 28 189879.0 29 236949.0 30 288831.0 31 351115.0 32 427952.0 33 528294.0 34 681802.0 35 940973.0 36 1216750.0 37 1703984.0 38 1575372.0 39 338478.0 40 600.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.689727802859874 31.21691248169211 28.595746119359028 22.497613596088982 2 22.507758756684943 29.634461280020492 27.83515174715014 20.02262821614443 3 21.5991481896092 28.542238108517815 28.173432150117378 21.68518155175561 4 21.778650376573474 28.65555676842136 28.25217445358556 21.31361840141961 5 22.18407513305613 28.738607586148706 28.233747285740378 20.843569995054786 6 22.0441990720566 28.676067861201542 27.623309237565476 21.656423829176386 7 22.165764871660432 28.767184153415126 28.0714816777307 20.995569297193743 8 21.533130534559298 28.8321454392118 28.039282053462017 21.595441972766892 9 21.271909165732637 28.594495950605353 28.579948762282065 21.553646121379945 10-11 21.35084558822155 29.046385444594087 28.00023647215586 21.602532495028505 12-13 21.318506401020993 29.05122344140498 27.885089547859703 21.745180609714325 14-15 21.4664293121379 29.082715603824433 27.75942529927701 21.691429784760654 16-17 21.642681013608815 28.889633811298314 27.721892279270854 21.74579289582202 18-19 21.61244152494703 28.882191589739076 27.71014584391283 21.79522104140106 20-21 21.71678284109001 28.8086241859406 27.751989127859794 21.722603845109596 22-23 21.78202038507201 28.810024879342905 27.698238088374406 21.709716647210684 24-25 21.67627915481594 28.763548385408527 27.675367874063824 21.884804585711713 26-27 21.839403890132868 28.65324847333334 27.808406318028045 21.698941318505742 28-29 21.843783260715302 28.726523265657562 27.71285268942182 21.71684078420532 30-31 21.819294698903715 28.738929902798155 27.6630851638454 21.77869023445273 32-33 21.846648086058313 28.755877689689385 27.595669314445193 21.801804909807114 34-35 21.851627494969726 28.774859577721422 27.605847479699335 21.767665447609513 36-37 21.869982866412865 28.764180949862826 27.579302319135664 21.78653386458864 38-39 21.89299225199324 28.7653068820983 27.575314255099876 21.76638661080859 40-41 21.880876098930134 28.819474651888743 27.550401504665846 21.749247744515277 42-43 21.87881407607757 28.789814454928443 27.545432999203314 21.785938469790675 44-45 21.893327119943958 28.8045443473813 27.527305117346707 21.774823415328033 46-47 21.87908425782331 28.840749201260635 27.48487507748924 21.795291463426814 48-49 21.927235842396666 28.825284477665473 27.47582811737093 21.771651562566927 50-51 21.92616883324966 28.805039575299883 27.480196327668526 21.788595263781925 52-53 21.872557081635108 28.885915992771043 27.41760121146318 21.823925714130667 54-55 21.93504252214496 28.88559925307061 27.34428832619477 21.83506989858966 56-57 21.945012383094905 28.892939299470633 27.343571805261647 21.818476512172815 58-59 21.895580913777152 28.958973352505534 27.348347999535385 21.797097734181932 60-61 21.918712936492526 28.941706222792146 27.33063922314269 21.80894161757264 62-63 21.904103563191608 28.97354766125314 27.337640064120862 21.784708711434387 64-65 21.88630784472336 29.03780159570767 27.308204187222625 21.76768637234635 66-67 21.8966702908595 29.05570702494391 27.261451200579074 21.786171483617515 68-69 21.853425923898502 29.07854479076601 27.289349432541883 21.778679852793605 70-71 21.894945188257502 29.11604226049459 27.244428558298623 21.744583992949284 72-73 21.86872153184756 29.105212592913787 27.235937766783575 21.79012810845508 74-75 21.91782819113107 29.127788154044598 27.21930154031888 21.73508211450546 76-77 21.853196261682243 29.19188251001335 27.245425901201603 21.709495327102804 78-79 21.84210794744113 29.1948729673079 27.20380573910267 21.7592133461483 80-81 21.842743720637877 29.210354158533704 27.198213319676178 21.74868880115224 82-83 21.880394728360567 29.28393601731002 27.13747598982893 21.698193264500482 84-85 21.803353541308862 29.30065665239719 27.170440174993377 21.725549631300574 86-87 21.833288259767293 29.319895429326788 27.134532418361307 21.71228389254462 88-89 21.814101280356738 29.363677387484817 27.11401449913886 21.708206833019585 90-91 21.814303603935528 29.357003079233756 27.090170296969635 21.73852301986108 92-93 21.79817397238283 29.424527121949893 27.068614833908306 21.70868407175897 94-95 21.807747014194533 29.448550816663282 27.06578625115874 21.677915917983448 96-97 21.79025204906958 29.466057401932083 27.039136379842144 21.704554169156197 98-99 21.803023543351433 29.48523849635653 26.984477483412068 21.72726047687997 100-101 21.86196245989507 29.45064456802562 26.946993616214765 21.740399355864547 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 139233.0 1 78282.0 2 12369.5 3 6569.0 4 5461.0 5 4961.5 6 4683.0 7 4435.0 8 4041.0 9 3930.5 10 3989.0 11 4488.5 12 8191.0 13 8082.5 14 4818.0 15 5194.0 16 5872.5 17 6668.0 18 8297.0 19 10442.0 20 13404.0 21 17890.5 22 23654.0 23 30765.0 24 39752.0 25 50772.5 26 65271.0 27 80878.5 28 93022.5 29 105531.0 30 122048.5 31 139457.0 32 157524.0 33 178462.5 34 200644.0 35 220453.5 36 240022.5 37 258187.0 38 271252.5 39 279116.0 40 287892.0 41 299792.5 42 309780.5 43 321116.0 44 333608.5 45 344811.5 46 351749.0 47 353803.0 48 355658.5 49 357654.0 50 356694.5 51 347964.5 52 334773.5 53 319494.5 54 297807.0 55 273184.5 56 247361.0 57 220215.0 58 192869.0 59 161386.5 60 127378.0 61 97311.0 62 72320.0 63 52426.0 64 37094.5 65 24569.0 66 15192.0 67 9437.0 68 5383.5 69 2574.5 70 1218.5 71 618.5 72 330.5 73 198.0 74 134.0 75 85.5 76 61.5 77 49.5 78 31.0 79 23.5 80 20.0 81 19.0 82 16.0 83 11.5 84 7.5 85 3.5 86 2.0 87 4.0 88 4.5 89 3.5 90 3.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15914302867554442 2 0.0 3 0.0 4 6.942481116718381E-4 5 6.088021902353042E-4 6 7.476518125696718E-5 7 2.5633776430960176E-4 8 0.0 9 1.3884962233436763E-4 10-11 2.4031665404025167E-4 12-13 0.0 14-15 0.0 16-17 1.602111026935011E-5 18-19 5.34037008978337E-6 20-21 1.068074017956674E-5 22-23 6.461847808637878E-4 24-25 0.0 26-27 1.1214777188545076E-4 28-29 0.005687494145619289 30-31 0.004015958307517094 32-33 1.975936933219847E-4 34-35 8.918418049938229E-4 36-37 4.5393145763158646E-4 38-39 0.0011748814197523414 40-41 0.003230923904318939 42-43 0.0013244117822662758 44-45 7.529921826594551E-4 46-47 0.00922281914505588 48-49 0.008971821750836061 50-51 0.007978512914136355 52-53 0.015006439952291272 54-55 0.006237552264866976 56-57 9.025225451733895E-4 58-59 0.0014365595541517265 60-61 1.068074017956674E-5 62-63 1.6555147278328447E-4 64-65 9.879684666099235E-4 66-67 0.001094775868405591 68-69 0.0030119687306378205 70-71 0.0020453617443870306 72-73 0.005772940067055823 74-75 0.002162849886362265 76-77 0.0015700688063963108 78-79 0.001660855097922628 80-81 3.417836857461357E-4 82-83 0.0022269343274396655 84-85 7.476518125696718E-5 86-87 0.0018691295314241794 88-89 2.349762839504683E-4 90-91 9.6660698625079E-4 92-93 0.0014739421447802102 94-95 0.0010467125375975405 96-97 0.0011214777188545077 98-99 0.5276499263509561 100-101 2.136148035913348E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9362647.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.25890955838813 #Duplication Level Percentage of deduplicated Percentage of total 1 90.09876314878859 68.70833430286103 2 6.073668104457854 9.263426133310364 3 1.4487108287453956 3.3143132419665795 4 0.6095880695353134 1.8594608585026353 5 0.34855092712090835 1.3290056813902842 6 0.24050778527781813 1.1004516867353618 7 0.17623181903749896 0.9407472444503598 8 0.13273874686716036 0.8098009673789246 9 0.10237113027941774 0.7026039688831278 >10 0.7609094599294222 10.27300622122608 >50 0.007041202814438578 0.32777205314259694 >100 6.52777476971414E-4 0.09614617164844216 >500 3.799995282344419E-5 0.01863307882871828 >1k 1.1399985820218495E-4 0.24895300130791617 >5k 3.7999952734061646E-5 0.14932543542540938 >10k+ 7.599990546812329E-5 0.8580199529421592 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18899 0.20185530865363183 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10710 0.11439072732315979 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.136148035913348E-5 0.0 0.0 1.068074017956674E-5 2.136148035913348E-5 2 5.34037008978337E-5 0.0 0.0 1.068074017956674E-5 2.136148035913348E-5 3 5.34037008978337E-5 0.0 0.0 1.068074017956674E-5 2.136148035913348E-5 4 7.476518125696718E-5 0.0 0.0 1.068074017956674E-5 4.272296071826696E-5 5 7.476518125696718E-5 0.0 0.0 1.068074017956674E-5 5.34037008978337E-5 6 8.544592143653392E-5 0.0 0.0 2.136148035913348E-5 6.408444107740044E-5 7 8.544592143653392E-5 0.0 0.0 2.136148035913348E-5 6.408444107740044E-5 8 8.544592143653392E-5 0.0 0.0 2.136148035913348E-5 6.408444107740044E-5 9 1.1748814197523414E-4 0.0 0.0 5.34037008978337E-5 6.408444107740044E-5 10-11 1.2282851206501751E-4 0.0 0.0 5.34037008978337E-5 6.408444107740044E-5 12-13 1.2816888215480088E-4 0.0 0.0 5.34037008978337E-5 6.408444107740044E-5 14-15 1.3884962233436763E-4 0.0 0.0 5.34037008978337E-5 7.476518125696718E-5 16-17 1.4953036251393436E-4 0.0 0.0 5.34037008978337E-5 1.0146703170588404E-4 18-19 1.602111026935011E-4 0.0 0.0 5.34037008978337E-5 1.1214777188545076E-4 20-21 1.7089184287306784E-4 0.0 0.0 5.34037008978337E-5 1.1748814197523414E-4 22-23 1.815725830526346E-4 0.0 0.0 5.874407098761707E-5 1.2816888215480088E-4 24-25 2.0827443350155143E-4 0.0 0.0 6.408444107740044E-5 1.602111026935011E-4 26-27 2.3497628395046828E-4 0.0 0.0 6.408444107740044E-5 1.602111026935011E-4 28-29 2.4031665404025167E-4 0.0 0.0 6.408444107740044E-5 1.602111026935011E-4 30-31 2.5633776430960176E-4 0.0 0.0 8.544592143653392E-5 1.7089184287306784E-4 32-33 2.830396147585186E-4 0.0 0.0 1.068074017956674E-4 1.8157258305263456E-4 34-35 2.990607250278687E-4 0.0 0.0 1.1748814197523414E-4 2.136148035913348E-4 36-37 3.0974146520743544E-4 0.0 0.0 1.1748814197523414E-4 2.4031665404025167E-4 38-39 3.204222053870022E-4 0.0 0.0 1.2282851206501751E-4 2.4565702413003503E-4 40-41 3.417836857461357E-4 0.0 0.0 1.3884962233436763E-4 2.670185044891685E-4 42-43 3.6848553619505254E-4 0.0 0.0 1.44189992424151E-4 2.723588745789519E-4 44-45 4.379103473622363E-4 0.0 0.0 1.5487073260371775E-4 2.88379984848302E-4 46-47 4.8597367817028664E-4 0.0 0.0 1.602111026935011E-4 2.88379984848302E-4 48-49 5.660792295170373E-4 0.0 0.0 1.602111026935011E-4 3.044010951176521E-4 50-51 6.461847808637878E-4 0.0 0.0 1.602111026935011E-4 3.0974146520743544E-4 52-53 7.316307023003217E-4 0.0 0.0 1.602111026935011E-4 3.150818352972188E-4 54-55 8.170766237368556E-4 0.0 0.0 1.6555147278328447E-4 3.204222053870022E-4 56-57 9.452455058916565E-4 0.0 0.0 1.8157258305263456E-4 3.204222053870022E-4 58-59 0.0010734143880464573 0.0 0.0 2.0293406341176807E-4 3.204222053870022E-4 60-61 0.001238965860829742 0.0 0.0 2.0827443350155143E-4 3.204222053870022E-4 62-63 0.0013938365934334596 0.0 0.0 2.136148035913348E-4 3.204222053870022E-4 64-65 0.0015807495465758774 5.34037008978337E-6 0.0 2.2429554377090152E-4 3.3110294556656895E-4 66-67 0.0018798102716037464 1.068074017956674E-5 0.0 2.3497628395046828E-4 3.364433156563523E-4 68-69 0.0020720635948359477 1.068074017956674E-5 0.0 2.3497628395046828E-4 3.4712405583591904E-4 70-71 0.002323060989055766 1.068074017956674E-5 0.0 2.3497628395046828E-4 3.631451661052692E-4 72-73 0.0026061006038142842 1.068074017956674E-5 0.0 2.4565702413003503E-4 3.6848553619505254E-4 74-75 0.0029906072502786873 1.068074017956674E-5 0.0 2.670185044891685E-4 3.738259062848359E-4 76-77 0.00339113500701244 1.068074017956674E-5 0.0 2.670185044891685E-4 3.8450664646440263E-4 78-79 0.003823704984284893 1.068074017956674E-5 0.0 2.670185044891685E-4 3.9518738664396936E-4 80-81 0.004582037537034131 1.068074017956674E-5 0.0 2.670185044891685E-4 3.9518738664396936E-4 82-83 0.0054097949009505545 1.068074017956674E-5 0.0 2.670185044891685E-4 4.0586812682353614E-4 84-85 0.006451167068458311 1.068074017956674E-5 0.0 2.723588745789519E-4 4.1654886700310287E-4 86-87 0.008074639575752454 1.068074017956674E-5 0.0 2.7769924466873526E-4 4.1654886700310287E-4 88-89 0.009975811327715335 1.068074017956674E-5 0.0 2.7769924466873526E-4 4.1654886700310287E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 490 0.0 33.911263 3 CGCGTAG 330 0.0 25.897602 54-55 CTCGCGT 400 0.0 22.554663 52-53 CGTACGC 380 0.0 21.863605 20-21 ACTCGCG 395 0.0 21.638687 50-51 TCGCGTA 420 0.0 20.915352 52-53 CGTAGCG 420 0.0 20.912169 56-57 CAACGCA 6635 0.0 19.641129 12-13 ACGTACG 420 0.0 19.216175 20-21 ATCAACG 6920 0.0 19.038027 10-11 CGCTAGT 470 0.0 18.182888 90-91 AGTGGTA 8720 0.0 17.966652 5 CGACGAC 480 0.0 17.803507 60-61 TCAACGC 6390 0.0 17.682398 10-11 GGTATCA 8810 0.0 17.56737 8 GTATCAA 8980 0.0 17.393404 9 AAGCAGT 9845 0.0 16.71094 1 ACGCAGA 8035 0.0 16.484789 14-15 TGGTATC 10040 0.0 15.746194 7 GTGGTAT 10095 0.0 15.660403 6 >>END_MODULE