FastQCFastQC Report
Sat 18 Jun 2016
SRR578551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8762922
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100647311.485586657053434No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT245070.27966698779242816No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG108660.12399973433519093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA104550.11930951798954732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101980.11637670630869476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2264550.091.328225
AAGCAGT2276700.091.161121
GGTATCA2275100.091.147118
GTATCAA2276400.091.087759
TGGTATC2277300.090.979847
GTGGTAT2283100.090.796556
AGCAGTG2305400.090.021612
GCAGTGG2302200.089.977023
CAGTGGT2304800.089.7477344
TCAACGC2268950.046.230612-13
CAACGCA2268200.046.13612-13
ATCAACG2269700.046.11100810-11
AACGCAG2278800.046.0410314-15
ACGCAGA2281600.045.9075514-15
CGCAGAG2286250.045.88990816-17
TATCAAC2284600.045.84144610-11
AGAGTAC2235000.045.58018518-19
CAGAGTA2288850.045.3922118-19
GAGTACT1290150.045.2931620-21
GCAGAGT2315300.045.11733216-17
Produced by FastQC (version 0.11.5)