##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578551_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8762922 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.9342666749744 31.0 31.0 34.0 27.0 34.0 2 31.284722835602096 33.0 31.0 34.0 28.0 34.0 3 31.405112586874562 34.0 31.0 34.0 28.0 34.0 4 34.782792543400475 37.0 35.0 37.0 32.0 37.0 5 34.81270767901392 37.0 35.0 37.0 32.0 37.0 6 34.8503940808785 37.0 35.0 37.0 32.0 37.0 7 34.78754050304225 37.0 35.0 37.0 32.0 37.0 8 34.82431225566084 37.0 35.0 37.0 32.0 37.0 9 36.318496615626614 39.0 37.0 39.0 32.0 39.0 10-11 36.20448521623267 39.0 37.0 39.0 32.0 39.0 12-13 36.255060355438516 39.0 37.0 39.0 32.0 39.0 14-15 37.50354533567685 40.0 38.0 41.0 32.0 41.0 16-17 37.476608088032734 40.0 38.0 41.0 32.0 41.0 18-19 37.40273324354593 40.0 38.0 41.0 31.5 41.0 20-21 37.335118924943075 40.0 37.5 41.0 31.0 41.0 22-23 37.07156653910648 39.5 37.0 41.0 31.0 41.0 24-25 37.02224075485323 40.0 37.0 41.0 31.0 41.0 26-27 36.909630657445085 40.0 37.0 41.0 30.0 41.0 28-29 36.74702895906183 40.0 36.5 41.0 30.0 41.0 30-31 36.51384172996177 40.0 36.0 41.0 29.5 41.0 32-33 36.234571128214995 39.0 36.0 41.0 29.0 41.0 34-35 36.06841245420192 39.0 35.5 41.0 27.0 41.0 36-37 35.79351647772285 39.0 35.0 40.5 27.0 41.0 38-39 34.95815973256409 38.0 35.0 40.0 26.5 41.0 40-41 34.72459431910954 38.0 34.0 40.0 25.5 41.0 42-43 34.43072054047725 37.5 34.0 40.0 24.0 41.0 44-45 34.14827228862701 37.0 33.0 40.0 24.0 41.0 46-47 34.008432290051196 37.0 33.0 40.0 23.0 41.0 48-49 34.03354702917588 38.0 33.0 40.0 23.0 41.0 50-51 33.15755075761258 37.0 32.5 39.5 19.0 40.5 52-53 32.75753395956281 37.5 33.0 39.5 10.5 40.5 54-55 32.39354641066073 38.0 32.5 40.0 2.0 41.0 56-57 31.886353262073996 38.0 31.0 40.0 2.0 41.0 58-59 31.592247654378298 37.5 31.0 40.0 2.0 41.0 60-61 31.323888424431942 37.0 31.0 40.0 2.0 41.0 62-63 30.987864493145096 36.5 30.5 40.0 2.0 41.0 64-65 30.69149131990448 36.0 30.0 39.5 2.0 41.0 66-67 30.359436897874936 35.5 29.5 39.0 2.0 41.0 68-69 30.029975218311883 35.0 29.0 39.0 2.0 41.0 70-71 29.728779909258577 35.0 29.0 38.5 2.0 40.5 72-73 29.404538805663226 35.0 29.0 37.5 2.0 40.0 74-75 29.0392802766018 35.0 28.5 37.0 2.0 39.0 76-77 28.665840572357027 35.0 28.0 36.5 2.0 39.0 78-79 28.321696461522766 35.0 27.5 36.0 2.0 39.0 80-81 28.000487565677293 35.0 27.0 36.0 2.0 37.5 82-83 27.733827540630855 34.0 26.0 35.0 2.0 37.0 84-85 27.45796607569941 34.0 26.0 35.0 2.0 37.0 86-87 27.20910513639172 34.0 26.0 35.0 2.0 36.0 88-89 26.949431251356568 34.0 25.0 35.0 2.0 36.0 90-91 26.71040681407412 34.0 25.0 35.0 2.0 36.0 92-93 26.426941778096392 34.0 24.5 35.0 2.0 35.0 94-95 26.144103188411357 34.0 23.5 35.0 2.0 35.0 96-97 25.86969586172284 34.0 20.0 35.0 2.0 35.0 98-99 25.565396793444016 34.0 18.5 35.0 2.0 35.0 100-101 24.714816359200732 33.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5552269760947901 1101 2 0.5571137596757332 1101 3 0.46001988446586495 1101 4 0.5259212471841508 1101 5 0.46857891393800344 1101 6 0.46795495770817297 1101 7 0.3979781543227361 1101 8 0.4160070753106595 1101 9 0.5316935280874446 1101 10-11 0.6354500305429553 1101 12-13 0.6472963867664063 1101 14-15 0.7746545069371962 1101 16-17 0.7064965690344778 1101 18-19 0.7474524770991096 1101 20-21 0.8113463449811462 1101 22-23 0.840386348063376 1101 24-25 0.7801228885927429 1101 26-27 0.8330241136577143 1101 28-29 0.9258935062797207 1101 30-31 0.9870824310443922 1101 32-33 0.983721380687129 1101 34-35 1.0639145601891968 1101 36-37 0.9464047605157759 1101 38-39 1.0236440847893036 1101 40-41 0.9399188731664907 1101 42-43 0.9651424226663181 1101 44-45 1.1899988057090027 1101 46-47 1.056895727058361 1101 48-49 1.0258503544165052 1101 50-51 1.1310012601284853 1101 52-53 0.9802311049052292 1101 54-55 0.8772646313711832 1101 56-57 0.9356442027503284 1101 58-59 0.9590215926008341 1101 60-61 0.8788907153540713 1101 62-63 0.8255960866584537 1101 64-65 0.9016604245355708 1101 66-67 0.8894860626458545 1101 68-69 0.8189521670863265 1101 70-71 0.9361901435342048 1101 72-73 0.9006569549400076 1101 74-75 0.8759951439255573 1101 76-77 0.8914296418579468 1101 78-79 0.8814189392351466 1101 80-81 0.8541730354117227 1101 82-83 0.9077329670017278 1101 84-85 0.8645554129665349 1101 86-87 0.8774835279484776 1101 88-89 0.8387600379719977 1101 90-91 0.912974194750845 1101 92-93 1.000514449793723 1101 94-95 0.9915539284609522 1101 96-97 0.9873172384625768 1101 98-99 0.940391741879246 1101 100-101 1.0257988350636182 1102 1 0.5442041302436031 1102 2 0.49994429167304943 1102 3 0.5398956274877058 1102 4 0.5303113782755773 1102 5 0.5662364220815661 1102 6 0.5743103513044403 1102 7 0.5515225542688569 1102 8 0.5122366682768629 1102 9 0.7004073804313791 1102 10-11 0.670437274560399 1102 12-13 0.6575072260126475 1102 14-15 0.8719537096646519 1102 16-17 0.8539004075633301 1102 18-19 0.9385100331144471 1102 20-21 0.9368197514727257 1102 22-23 0.938105150454021 1102 24-25 1.0072182659016562 1102 26-27 0.9939379398923478 1102 28-29 1.0697939709216016 1102 30-31 1.0459301352211057 1102 32-33 1.13877659627547 1102 34-35 1.1591172839498967 1102 36-37 1.1152664512442172 1102 38-39 1.1620338472890168 1102 40-41 1.230375279211799 1102 42-43 1.203719962903378 1102 44-45 1.2751462515344585 1102 46-47 1.2858647782878165 1102 48-49 1.2674330585430695 1102 50-51 1.2660216021059583 1102 52-53 1.1219644555332167 1102 54-55 1.1358910080212894 1102 56-57 1.1280643263694863 1102 58-59 1.1705233909139992 1102 60-61 1.2179199074734264 1102 62-63 1.242488635960978 1102 64-65 1.2616718364903932 1102 66-67 1.182652465718391 1102 68-69 1.1502862719033722 1102 70-71 1.159111209939816 1102 72-73 1.1422362193567004 1102 74-75 1.060347154942253 1102 76-77 1.1199636613992254 1102 78-79 1.0997570243634556 1102 80-81 1.1520085421456159 1102 82-83 1.170923489492722 1102 84-85 1.1769965552526322 1102 86-87 1.1769711705453254 1102 88-89 1.1827016232218526 1102 90-91 1.211151048612642 1102 92-93 1.1733301319585614 1102 94-95 1.2409607816068409 1102 96-97 1.3451902882296203 1102 98-99 1.3825414602259478 1102 100-101 1.4291204671262356 1103 1 0.392450427296108 1103 2 0.4343921661006682 1103 3 0.3553046748211095 1103 4 0.45140680448693615 1103 5 0.468238969419545 1103 6 0.4663028483960119 1103 7 0.3890856895432364 1103 8 0.4023641806860496 1103 9 0.5464114183234443 1103 10-11 0.5037218315258656 1103 12-13 0.5651415119106034 1103 14-15 0.61778971386547 1103 16-17 0.6778757527917492 1103 18-19 0.7001134826740412 1103 20-21 0.7191358445515448 1103 22-23 0.7892907734364485 1103 24-25 0.7004624456883093 1103 26-27 0.7859567065157194 1103 28-29 0.8491111573092383 1103 30-31 0.8790054678131938 1103 32-33 0.9194664665250727 1103 34-35 0.9026155999517869 1103 36-37 0.983242726290257 1103 38-39 0.8661048298390526 1103 40-41 0.9899626601144291 1103 42-43 0.8288631567063192 1103 44-45 0.78856920381903 1103 46-47 1.09729842686491 1103 48-49 1.0827162835149196 1103 50-51 0.9897899518381834 1103 52-53 0.9544117766874649 1103 54-55 0.9419012832188187 1103 56-57 0.92622735439247 1103 58-59 0.9136295330604014 1103 60-61 0.9055439134709111 1103 62-63 0.7922898635946503 1103 64-65 0.7651645731309955 1103 66-67 0.7975184773055268 1103 68-69 0.9389190612178275 1103 70-71 0.8378815004097433 1103 72-73 0.9272335522073583 1103 74-75 0.9159133070993839 1103 76-77 0.9292623511209115 1103 78-79 0.9385180303908598 1103 80-81 1.000591115265685 1103 82-83 1.019273713722324 1103 84-85 0.9975254513959442 1103 86-87 1.051374686297283 1103 88-89 1.0682856478655296 1103 90-91 1.0445193224598768 1103 92-93 1.0967096447867029 1103 94-95 1.034215722576029 1103 96-97 1.0057474079071653 1103 98-99 1.0655552059333182 1103 100-101 1.174620490031348 1104 1 0.34408546045412436 1104 2 0.30044521892481413 1104 3 0.33832548433403176 1104 4 0.28979006540621555 1104 5 0.3951769982327207 1104 6 0.41753769861325196 1104 7 0.3954314476040679 1104 8 0.2829953263526406 1104 9 0.42555617021002234 1104 10-11 0.4560714770781189 1104 12-13 0.5072007389858371 1104 14-15 0.6161318131108331 1104 16-17 0.628024392169749 1104 18-19 0.6126449256193851 1104 20-21 0.6539346097013308 1104 22-23 0.6068073524439939 1104 24-25 0.6081916933443878 1104 26-27 0.6803941636389794 1104 28-29 0.7025584474990367 1104 30-31 0.8263242421100117 1104 32-33 0.8245087715788131 1104 34-35 0.8502773881978456 1104 36-37 0.8631878440583876 1104 38-39 0.8935173612819938 1104 40-41 0.833033778339896 1104 42-43 0.9511476296082151 1104 44-45 0.9214871091471721 1104 46-47 1.0702003248754295 1104 48-49 0.9736378476585301 1104 50-51 0.9963929703609367 1104 52-53 0.858425039893504 1104 54-55 0.7783979674173125 1104 56-57 0.7419431101665346 1104 58-59 0.8060660754797979 1104 60-61 0.8454021343718914 1104 62-63 0.7364357588610595 1104 64-65 0.6985626979880735 1104 66-67 0.7918015781516239 1104 68-69 0.8219227200332853 1104 70-71 0.7024767439496458 1104 72-73 0.6808351986743162 1104 74-75 0.7323722597634585 1104 76-77 0.752181399828892 1104 78-79 0.6801999421219378 1104 80-81 0.8088749021396815 1104 82-83 0.7647859478865122 1104 84-85 0.7437522979543694 1104 86-87 0.7730760354854809 1104 88-89 0.8005346759926759 1104 90-91 0.7788550187781915 1104 92-93 0.5981960385667158 1104 94-95 0.4963984346729866 1104 96-97 0.615226026704331 1104 98-99 0.8576601682017859 1104 100-101 0.828719506724692 1105 1 0.2785684340914081 1105 2 0.18870078368572507 1105 3 0.21390675239849344 1105 4 0.23948358962774563 1105 5 0.1670287862163562 1105 6 0.15934853735421228 1105 7 0.1651901632198829 1105 8 0.15445732802960066 1105 9 0.09985297911813262 1105 10-11 0.17839921722224972 1105 12-13 0.20159785916818151 1105 14-15 0.043022813399495874 1105 16-17 0.06877174396461072 1105 18-19 -0.023511056381444462 1105 20-21 0.0722534039916809 1105 22-23 0.058462872719303505 1105 24-25 0.01499809602152169 1105 26-27 -0.3324061984937785 1105 28-29 -0.12350729496683499 1105 30-31 -0.25087628921796323 1105 32-33 -0.24389611983759352 1105 34-35 -0.14045358764872873 1105 36-37 -0.08964446398188386 1105 38-39 -0.0957651022704269 1105 40-41 -0.04621036636824982 1105 42-43 0.07568548547737919 1105 44-45 0.01807590102580292 1105 46-47 0.027665751693490392 1105 48-49 0.15557935659500544 1105 50-51 0.10622948578906488 1105 52-53 0.012754788137513628 1105 54-55 5.153381685332192E-4 1105 56-57 0.14228112573399088 1105 58-59 0.11702789534061964 1105 60-61 -0.0017625398148375382 1105 62-63 0.046360670670861026 1105 64-65 -0.05956248398426567 1105 66-67 -0.04093334891573619 1105 68-69 -0.006943565756689196 1105 70-71 -0.00677582927976772 1105 72-73 0.03608832297532061 1105 74-75 0.0495876362853096 1105 76-77 0.06059516962092104 1105 78-79 0.07585378668975551 1105 80-81 0.13615981864796112 1105 82-83 0.10724600144417451 1105 84-85 0.07713041054042336 1105 86-87 0.1475948600160386 1105 88-89 0.22030488788657365 1105 90-91 0.22349557889685556 1105 92-93 0.2539802409312024 1105 94-95 0.3007887882960034 1105 96-97 0.344534488089959 1105 98-99 0.35535198422634195 1105 100-101 0.3402331166934651 1106 1 -0.18154119599217466 1106 2 -0.13632211265937144 1106 3 -0.1598812734088355 1106 4 -0.18009287054623258 1106 5 -0.22464647721928088 1106 6 -0.2111568845683962 1106 7 -0.2486468897055687 1106 8 -0.20625918287382916 1106 9 -0.21613667071247988 1106 10-11 -0.371405014771355 1106 12-13 -0.3958318771408216 1106 14-15 -0.4031053210165183 1106 16-17 -0.405007174374596 1106 18-19 -0.40693376206552045 1106 20-21 -0.20709877414018507 1106 22-23 -0.2105145373550812 1106 24-25 -0.33265856094185864 1106 26-27 -0.32915032119775844 1106 28-29 -0.3846155972326315 1106 30-31 -0.42880957488605276 1106 32-33 -0.3071409319854226 1106 34-35 -0.24619495257731927 1106 36-37 -0.37416202352089556 1106 38-39 -0.38241134097120977 1106 40-41 -0.3816753579417167 1106 42-43 -0.47212923287203523 1106 44-45 -0.4623553844039492 1106 46-47 -0.4876428808244242 1106 48-49 -0.6335305799080615 1106 50-51 -0.7283426334421534 1106 52-53 -0.711075540582506 1106 54-55 -0.6260614454482436 1106 56-57 -0.5565123475309015 1106 58-59 -0.5635508970261291 1106 60-61 -0.6864039438970266 1106 62-63 -0.48568399848121047 1106 64-65 -0.47274405455437574 1106 66-67 -0.6277515229960535 1106 68-69 -0.7268691739142596 1106 70-71 -0.6638484166518808 1106 72-73 -0.7161327076155537 1106 74-75 -0.7847138123979356 1106 76-77 -0.819558465705839 1106 78-79 -0.7365745026383337 1106 80-81 -0.7207299384294785 1106 82-83 -0.786187343805473 1106 84-85 -0.8188451950636519 1106 86-87 -0.8152291360345565 1106 88-89 -0.9192173530612848 1106 90-91 -0.8023861431806694 1106 92-93 -0.8134604754094674 1106 94-95 -0.8812616687797359 1106 96-97 -0.7886664039312201 1106 98-99 -0.8357458487069387 1106 100-101 -0.971620040278431 1107 1 -0.42413815246410635 1107 2 -0.5465400103435556 1107 3 -0.4477137279043859 1107 4 -0.3649626842162661 1107 5 -0.40862151568973104 1107 6 -0.4097274485496669 1107 7 -0.33861341221747665 1107 8 -0.36104147752875804 1107 9 -0.38177261982636423 1107 10-11 -0.4198318039522775 1107 12-13 -0.5015643113123716 1107 14-15 -0.7169204292489368 1107 16-17 -0.5497713157806388 1107 18-19 -0.5324077574500095 1107 20-21 -0.4582990645670151 1107 22-23 -0.5109876234524648 1107 24-25 -0.5400259169162851 1107 26-27 -0.43017207690066783 1107 28-29 -0.4782835419324698 1107 30-31 -0.4932894584679488 1107 32-33 -0.45512796948190015 1107 34-35 -0.4583739519548047 1107 36-37 -0.651653155744242 1107 38-39 -0.6452634119603786 1107 40-41 -0.766725027029338 1107 42-43 -0.6411262792349959 1107 44-45 -0.5894758052524125 1107 46-47 -0.6314767324251989 1107 48-49 -0.6947169757989897 1107 50-51 -0.8262008954449342 1107 52-53 -0.8419957883991458 1107 54-55 -0.7265821603711693 1107 56-57 -0.7299403840336218 1107 58-59 -0.7181526470919799 1107 60-61 -0.6731341537479132 1107 62-63 -0.6458146334277508 1107 64-65 -0.690595089952005 1107 66-67 -0.7495919311071653 1107 68-69 -0.7247359916011966 1107 70-71 -0.6956314102262837 1107 72-73 -0.7719046813639387 1107 74-75 -0.7826698528996303 1107 76-77 -0.7423759067444706 1107 78-79 -0.7228092715551995 1107 80-81 -0.6792191345787941 1107 82-83 -0.7066166562450142 1107 84-85 -0.7856803497488478 1107 86-87 -0.8246048844931408 1107 88-89 -0.8748155298122349 1107 90-91 -0.7641274243227052 1107 92-93 -0.7590045283520972 1107 94-95 -0.7084588706700856 1107 96-97 -0.78369965501469 1107 98-99 -0.8332837874667938 1107 100-101 -0.95205935906883 1108 1 -0.7186841305601668 1108 2 -0.7199880843877047 1108 3 -0.6100425393361988 1108 4 -0.5880688274667918 1108 5 -0.8047134425451858 1108 6 -0.6652952747244072 1108 7 -0.5197858422217934 1108 8 -0.6542084112322542 1108 9 -0.7255570737360557 1108 10-11 -0.9628321125619479 1108 12-13 -0.842603787186512 1108 14-15 -0.8665688497549127 1108 16-17 -0.9272738782144998 1108 18-19 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-0.3695225238159985 2305 70-71 -0.38253122018772245 2305 72-73 -0.3253791654859093 2305 74-75 -0.3628515417719811 2305 76-77 -0.4149334129957012 2305 78-79 -0.3650341394598371 2305 80-81 -0.2872566542220767 2305 82-83 -0.35214649135451026 2305 84-85 -0.3417312632527789 2305 86-87 -0.27707898902001915 2305 88-89 -0.21704469501978352 2305 90-91 -0.22079160705076006 2305 92-93 -0.23190048746492664 2305 94-95 -0.1288746850352709 2305 96-97 -0.2059735411158563 2305 98-99 -0.16686669870869864 2305 100-101 -0.09777846153328085 2306 1 -0.33160530440292746 2306 2 -0.30871161217719134 2306 3 -0.3119030132505074 2306 4 -0.44796805018706465 2306 5 -0.44367479821958256 2306 6 -0.34824828681416875 2306 7 -0.41865146482007987 2306 8 -0.39941833468609644 2306 9 -0.5629893148898688 2306 10-11 -0.6916333144582865 2306 12-13 -0.7015771226879224 2306 14-15 -0.8071985836054765 2306 16-17 -0.6611898120201616 2306 18-19 -0.6003738431614991 2306 20-21 -0.6291258833505537 2306 22-23 -0.5978082377201162 2306 24-25 -0.6495352973209023 2306 26-27 -0.6962592138675063 2306 28-29 -0.5719169350617648 2306 30-31 -0.6259579630287035 2306 32-33 -0.770094870607771 2306 34-35 -0.6648213415096009 2306 36-37 -0.7132878184457283 2306 38-39 -0.6891978353467891 2306 40-41 -0.770708811612451 2306 42-43 -0.7793185640290119 2306 44-45 -0.872834797808828 2306 46-47 -0.7846986673448839 2306 48-49 -0.8751490258776826 2306 50-51 -0.8244240015749753 2306 52-53 -0.9421582152897656 2306 54-55 -0.8836007428836012 2306 56-57 -1.0017811436614217 2306 58-59 -0.8917336892093672 2306 60-61 -0.7972780727027562 2306 62-63 -0.89601968343813 2306 64-65 -0.9638009996339605 2306 66-67 -0.9185015940318344 2306 68-69 -0.9363748080168186 2306 70-71 -0.9289293916551742 2306 72-73 -0.8926266050608227 2306 74-75 -0.9950520379162597 2306 76-77 -0.967338903972145 2306 78-79 -1.0327329660268454 2306 80-81 -1.1307940778740004 2306 82-83 -1.1713414128117492 2306 84-85 -1.1090568117306994 2306 86-87 -1.132865147856002 2306 88-89 -1.0832377409876521 2306 90-91 -1.0428781946950494 2306 92-93 -1.0516061790781777 2306 94-95 -1.103374226064659 2306 96-97 -1.046979675771393 2306 98-99 -1.0054695313574733 2306 100-101 -1.1318688096315377 2307 1 -0.6077742351274189 2307 2 -0.6322053003547801 2307 3 -0.6453907293000114 2307 4 -0.7766085444064856 2307 5 -0.7141135344411609 2307 6 -0.5630498315299448 2307 7 -0.6068715553330435 2307 8 -0.5529252112611118 2307 9 -0.7012590447631695 2307 10-11 -0.7708293008661116 2307 12-13 -0.7544247581981054 2307 14-15 -0.9597413694980688 2307 16-17 -0.8734687134276911 2307 18-19 -0.9662016556128208 2307 20-21 -0.8045087056135856 2307 22-23 -0.8096724187084305 2307 24-25 -0.8531660353472432 2307 26-27 -0.9466709621020399 2307 28-29 -0.9202854432483818 2307 30-31 -0.8929565824195365 2307 32-33 -1.0994364640553727 2307 34-35 -0.9529584134152813 2307 36-37 -1.0175116842459246 2307 38-39 -0.9293271250051518 2307 40-41 -1.07540037949326 2307 42-43 -1.2528739203496926 2307 44-45 -1.1850627694502194 2307 46-47 -1.2096520933235269 2307 48-49 -1.2746269541991921 2307 50-51 -1.2433626610599156 2307 52-53 -1.0875868464585494 2307 54-55 -0.9854276186403581 2307 56-57 -0.9829838698887521 2307 58-59 -1.0673719155405905 2307 60-61 -1.0545392811712873 2307 62-63 -0.9798256383157913 2307 64-65 -1.0520310170186775 2307 66-67 -0.9777787139541196 2307 68-69 -0.9215916928466754 2307 70-71 -0.9547423166747713 2307 72-73 -0.9855351512258785 2307 74-75 -0.9763652013733406 2307 76-77 -0.9489714691230731 2307 78-79 -0.9739993157621782 2307 80-81 -0.9644842913538838 2307 82-83 -1.0130520360099773 2307 84-85 -0.9886005700040847 2307 86-87 -1.1285574156819251 2307 88-89 -1.1678950573472129 2307 90-91 -1.1428068385053116 2307 92-93 -1.0649780679529322 2307 94-95 -0.9768183599086484 2307 96-97 -1.0686414328524556 2307 98-99 -1.1916474208992902 2307 100-101 -1.2435885464457748 2308 1 -1.6228590450770284 2308 2 -1.23224678967307 2308 3 -1.166730450649169 2308 4 -1.1135902502590866 2308 5 -1.4494949926440697 2308 6 -1.466726721489259 2308 7 -1.4208684408159087 2308 8 -1.3065673329035263 2308 9 -1.375311195785926 2308 10-11 -1.454390588111636 2308 12-13 -1.298676339154298 2308 14-15 -1.8749820890503273 2308 16-17 -1.9543865421182645 2308 18-19 -1.6804475639122458 2308 20-21 -2.860642041193259 2308 22-23 -3.0320948737038265 2308 24-25 -2.2837506936878995 2308 26-27 -2.531028499611054 2308 28-29 -2.212380634329982 2308 30-31 -2.142847168589064 2308 32-33 -2.4517556142734875 2308 34-35 -2.5717292882018867 2308 36-37 -2.8709931598637297 2308 38-39 -2.9008101774355524 2308 40-41 -2.561892588240525 2308 42-43 -2.6625905577910416 2308 44-45 -2.642011924227333 2308 46-47 -2.736304828660593 2308 48-49 -2.491588970193206 2308 50-51 -2.4865070763782953 2308 52-53 -2.398558235949576 2308 54-55 -2.1605682714394803 2308 56-57 -2.0714713687082202 2308 58-59 -1.986187841583778 2308 60-61 -1.9388384557811627 2308 62-63 -1.9669293917681792 2308 64-65 -2.0051173061969436 2308 66-67 -2.165894729549823 2308 68-69 -1.9709367015574095 2308 70-71 -1.9532348879973114 2308 72-73 -1.954578068584702 2308 74-75 -1.764196095540406 2308 76-77 -1.66473724494967 2308 78-79 -1.7450397107621392 2308 80-81 -2.3764147535282305 2308 82-83 -2.1458911803040266 2308 84-85 -1.8568042231871011 2308 86-87 -1.7614080900286062 2308 88-89 -1.6962648840772268 2308 90-91 -1.8596523968706755 2308 92-93 -1.8958908107319594 2308 94-95 -1.9673250382450007 2308 96-97 -1.9653277923159926 2308 98-99 -1.9994429184131128 2308 100-101 -2.105614200769434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102866.0 3 16362.0 4 13993.0 5 13027.0 6 14686.0 7 17674.0 8 21288.0 9 24211.0 10 27400.0 11 32200.0 12 31880.0 13 35728.0 14 39051.0 15 46101.0 16 58363.0 17 78750.0 18 121539.0 19 197178.0 20 282179.0 21 200054.0 22 108898.0 23 90960.0 24 93911.0 25 99839.0 26 107160.0 27 119033.0 28 136400.0 29 157670.0 30 185952.0 31 222759.0 32 273188.0 33 344853.0 34 454264.0 35 661862.0 36 1078295.0 37 1498629.0 38 1453319.0 39 301009.0 40 391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.876447239448375 46.55748111688266 24.765949818946996 17.80012182472197 2 17.214429349859135 47.845711978407266 19.034471966505212 15.905386705228391 3 42.818273115418364 21.0160060678762 19.65068823819041 16.515032578515026 4 16.94648018420194 19.75712171454134 20.70609373098214 42.59030437027458 5 16.842701703418236 45.68744236449646 21.478132373261282 15.99172355882402 6 42.631372212481565 20.763106637458385 19.69990911593219 16.905612034127856 7 17.436816294661277 20.131325288968014 46.620363465144386 15.811494951226324 8 42.23828156784954 20.86420780190674 20.53511218660339 16.362398443640338 9 41.87638915122468 20.555175446054854 21.1741712455754 16.394264157145066 10-11 16.47783435354216 33.34170081470242 33.50646734599431 16.67399748576111 12-13 16.30886026047038 20.88144174370851 33.47525289426592 29.33444510155519 14-15 16.465132607658926 46.18179226387463 20.591814452508316 16.761260675958127 16-17 29.222219267810907 20.698159981220595 20.681475799205984 29.398144951762518 18-19 16.641239301877047 33.330602112162126 20.67867841000476 29.34948017595607 20-21 29.404795161793174 33.345515351282614 20.707650284448693 16.54203920247552 22-23 29.284226843935468 20.59447752841301 33.51881822522438 16.602477402427144 24-25 16.55071316963929 33.164082880779 21.110159112581155 29.17504483700056 26-27 16.752565790059293 25.74348969358874 40.692949900248756 16.810994616103212 28-29 27.20011494060708 20.48291944577219 35.6150980056401 16.701867607980635 30-31 25.162811836928817 21.596241023886755 35.99565991069322 17.245287228491208 32-33 19.8796609252684 23.842832503281446 37.330104876167795 18.947401695282366 34-35 19.247148943318486 22.91744933876666 38.069269847385215 19.76613187052964 36-37 19.118031786204767 22.991657032762994 38.23894504204349 19.65136613898874 38-39 19.579800818275046 23.020309433112608 37.88556531339108 19.514324435221262 40-41 19.55176724809375 23.046012985819907 37.68505706331486 19.717162702771486 42-43 19.836814912293267 23.270353729548297 37.64526803358352 19.24756332457492 44-45 19.64030130795167 23.2914429098621 37.61082851698452 19.457427265201712 46-47 19.716398535860296 23.136899344674806 37.52765232673253 19.619049792732365 48-49 19.664241067737507 23.17574386615656 37.48767148722334 19.672343578882597 50-51 19.90747419009674 23.407017929072524 36.789463040802836 19.8960448400279 52-53 20.259214117021706 24.070521349931745 35.62666416507489 20.043600367971656 54-55 21.013326412895967 24.94189163801816 33.95047613431865 20.094305814767228 56-57 21.360937506873345 25.748412682135434 32.516806096909356 20.37384371408186 58-59 21.45695912037727 26.37810796178968 31.717980867876587 20.44695204995646 60-61 21.628662831783394 26.556894142096617 31.455309413940057 20.35913361217993 62-63 21.374579515485312 26.466684831254973 31.57550956525514 20.583226088004576 64-65 21.00308091463779 26.251443420075155 31.775251747027404 20.970223918259652 66-67 21.088971437793404 25.847067443026262 32.31641652063508 20.747544598545254 68-69 21.032317219107846 25.775735126704845 32.493947873927006 20.6979997802603 70-71 20.685524668642856 25.655454835609504 33.06629348040501 20.592727015342632 72-73 20.546596244822023 25.618747865389484 32.85190934073859 20.982746549049903 74-75 20.983705749727683 25.72270617679795 32.26194260075054 21.031645472723827 76-77 21.177279711396697 25.96376781703417 31.99817817565499 20.86077429591414 78-79 21.241348762020795 26.090356114191 31.54044962124344 21.12784550254477 80-81 20.9622412702131 26.420649532997288 31.51427147148348 21.102837725306134 82-83 21.527872903121658 26.624998243473634 30.90819781994228 20.938931033462428 84-85 21.30434154218495 26.994043489286263 30.556107618562805 21.145507349965982 86-87 21.374879026529246 26.92957291716117 30.594118631322676 21.101429424986907 88-89 21.26577994841971 26.75793805189368 30.46534513707474 21.510936862611874 90-91 21.445312002336426 26.79158030143055 30.42948657558442 21.333621120648605 92-93 21.43312022023319 27.09036649514423 30.33433533003179 21.142177954590792 94-95 21.50567122993017 26.904000256928583 30.01117890790036 21.579149605240886 96-97 21.223452440520507 26.902648909890246 30.39617412954315 21.477724520046092 98-99 21.507538498114585 26.887667044852858 30.027709466384938 21.57708499064762 100-101 21.739029339115216 26.780555017896617 29.788495891308138 21.69191975168003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11427.0 1 6611.0 2 1359.0 3 799.0 4 647.0 5 625.0 6 750.0 7 920.5 8 922.0 9 972.5 10 1532.5 11 7776.0 12 127606.0 13 142196.0 14 39016.0 15 36998.0 16 41086.0 17 45180.0 18 48590.0 19 51561.0 20 54243.5 21 57049.0 22 60355.0 23 64782.5 24 70205.0 25 76475.0 26 84341.0 27 93830.0 28 103087.5 29 111987.5 30 122771.5 31 134879.5 32 145689.5 33 158666.0 34 177495.0 35 191528.5 36 200901.5 37 211256.0 38 219132.5 39 226270.0 40 236990.5 41 245761.0 42 250797.5 43 259347.0 44 270875.5 45 279151.5 46 281685.0 47 284209.0 48 285505.0 49 288175.0 50 294087.0 51 291197.5 52 283222.5 53 281284.5 54 267511.0 55 244001.0 56 229201.5 57 216268.0 58 207005.5 59 172552.0 60 124450.0 61 95552.5 62 72298.0 63 53994.5 64 38380.0 65 26172.5 66 16801.5 67 10304.5 68 6603.5 69 4219.0 70 2976.5 71 2337.0 72 1983.5 73 1753.0 74 1545.5 75 1342.0 76 1179.5 77 1043.5 78 892.0 79 779.5 80 687.5 81 592.5 82 506.0 83 411.0 84 337.0 85 281.0 86 213.5 87 160.0 88 134.0 89 97.5 90 71.0 91 56.5 92 38.5 93 30.0 94 25.5 95 17.5 96 9.0 97 5.5 98 3.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050633795439466424 2 0.09274303708283607 3 0.08336260439154886 4 0.053726371180754545 5 0.08522271452376273 6 0.001255289046279312 7 0.001152583578856459 8 0.004827156968874081 9 0.009437491284299918 10-11 0.009437491284299918 12-13 0.017157518918917685 14-15 0.011451659617648087 16-17 0.0014949351369326351 18-19 6.048210859345776E-4 20-21 6.84703116152352E-5 22-23 3.879984324863328E-4 24-25 7.98820302177744E-4 26-27 0.0014150531067148606 28-29 0.0015462878706440613 30-31 0.004901333139790585 32-33 1.8258749764062717E-4 34-35 0.0023508140321230747 36-37 3.7088085458252397E-4 38-39 0.0025162839518598932 40-41 0.0032751632389287503 42-43 0.0012724066241831206 44-45 6.847031161523519E-4 46-47 0.0011069367044463023 48-49 0.0023565198914243446 50-51 0.004496217129400444 52-53 0.12736619132294 54-55 0.13728297478854656 56-57 0.12342344254576271 58-59 0.11708993872135344 60-61 0.10548992676187235 62-63 0.10986061498664487 64-65 0.11228560518968445 66-67 0.12021674961844918 68-69 0.13304352132770325 70-71 0.12034227852307712 72-73 0.11253666299894031 74-75 0.11712987973646234 76-77 0.12843889287157867 78-79 0.14278912901427174 80-81 0.1327696400812423 82-83 0.11241683995361364 84-85 0.11866475588850386 86-87 0.11966898712552732 88-89 0.11594876686109952 90-91 0.1265388417242559 92-93 0.1433368915071936 94-95 0.15301973474144812 96-97 0.1533278511437167 98-99 0.13627303769222185 100-101 0.1253919640047007 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8762922.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.08785597588444 #Duplication Level Percentage of deduplicated Percentage of total 1 91.24366367135094 53.001487940534744 2 5.462260985391854 6.345820588442693 3 1.3044048610716943 2.273102451125284 4 0.555511688630719 1.2907393184840628 5 0.3257388424953468 0.9460735484310504 6 0.23366182907155228 0.8143748804502033 7 0.15755280878775518 0.6406333405821429 8 0.12047241780473461 0.5598387563606401 9 0.0945767396880751 0.4944384030302659 >10 0.47774772957795947 4.140675810347817 >50 0.011074693590626673 0.48952522804442644 >100 0.008960787661754668 1.112016589795073 >500 0.0018575341169810471 0.7389588376412525 >1k 0.0021284244892661322 2.4419607543830506 >5k 1.9349313538783016E-4 0.8262230974975057 >10k+ 1.9349313538783016E-4 23.88413045484974 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1006473 11.485586657053434 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 24507 0.27966698779242816 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 10866 0.12399973433519093 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA 10455 0.11930951798954732 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10198 0.11637670630869476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.1411718602539198E-5 0.0 0.0 0.0 3 0.0 1.1411718602539198E-5 0.0 0.0 0.0 4 0.0 1.1411718602539198E-5 0.0 0.0 0.0 5 0.0 1.1411718602539198E-5 0.0 0.0 0.0 6 0.0 1.1411718602539198E-5 0.0 1.1411718602539198E-5 0.0 7 0.0 1.1411718602539198E-5 0.0 2.2823437205078396E-5 0.0 8 0.0 1.1411718602539198E-5 0.0 2.2823437205078396E-5 0.0 9 0.0 1.1411718602539198E-5 0.0 4.564687441015679E-5 0.0 10-11 0.0 1.1411718602539198E-5 0.0 5.135273371142639E-5 0.0 12-13 1.1411718602539198E-5 1.1411718602539198E-5 0.0 6.847031161523519E-5 0.0 14-15 1.1411718602539198E-5 1.1411718602539198E-5 0.0 7.417617091650479E-5 0.0 16-17 1.7117577903808797E-5 1.1411718602539198E-5 0.0 7.988203021777439E-5 0.0 18-19 2.2823437205078396E-5 1.1411718602539198E-5 0.0 8.558788951904399E-5 0.0 20-21 2.2823437205078396E-5 1.1411718602539198E-5 0.0 9.129374882031359E-5 0.0 22-23 2.8529296506347995E-5 1.1411718602539198E-5 0.0 9.129374882031359E-5 0.0 24-25 3.9941015108887194E-5 1.1411718602539198E-5 0.0 9.129374882031359E-5 0.0 26-27 4.564687441015679E-5 1.1411718602539198E-5 0.0 9.129374882031359E-5 0.0 28-29 5.135273371142639E-5 1.1411718602539198E-5 0.0 9.129374882031359E-5 1.1411718602539198E-5 30-31 6.847031161523519E-5 1.1411718602539198E-5 0.0 9.129374882031359E-5 1.1411718602539198E-5 32-33 6.847031161523519E-5 1.1411718602539198E-5 0.0 9.129374882031359E-5 1.1411718602539198E-5 34-35 6.847031161523519E-5 1.1411718602539198E-5 0.0 1.0270546742285278E-4 1.1411718602539198E-5 36-37 6.847031161523519E-5 1.1411718602539198E-5 0.0 1.0841132672412238E-4 1.7117577903808797E-5 38-39 9.129374882031359E-5 1.1411718602539198E-5 0.0 1.1411718602539198E-4 2.2823437205078396E-5 40-41 9.129374882031359E-5 1.1411718602539198E-5 0.0 1.1411718602539198E-4 2.2823437205078396E-5 42-43 1.0841132672412238E-4 1.7117577903808797E-5 0.0 1.1411718602539198E-4 2.2823437205078396E-5 44-45 1.4264648253173998E-4 2.2823437205078396E-5 0.0 1.1411718602539198E-4 2.2823437205078396E-5 46-47 1.7688163833935757E-4 2.2823437205078396E-5 0.0 1.1411718602539198E-4 2.2823437205078396E-5 48-49 2.3394023135205356E-4 2.2823437205078396E-5 0.0 1.3123476392920078E-4 2.2823437205078396E-5 50-51 2.795871057622104E-4 2.2823437205078396E-5 0.0 1.3694062323047038E-4 2.2823437205078396E-5 52-53 3.138222615698279E-4 2.2823437205078396E-5 0.0 1.5976406043554877E-4 2.2823437205078396E-5 54-55 3.822925731850631E-4 2.2823437205078396E-5 0.0 1.7117577903808797E-4 3.4235155807617595E-5 56-57 4.678804627041071E-4 2.2823437205078396E-5 0.0 1.8258749764062717E-4 4.564687441015679E-5 58-59 5.648800708256904E-4 2.2823437205078396E-5 0.0 1.8258749764062717E-4 5.135273371142639E-5 60-61 7.303499905625087E-4 2.2823437205078396E-5 0.0 1.8258749764062717E-4 5.705859301269599E-5 62-63 8.672906137929791E-4 2.2823437205078396E-5 0.0 1.8829335694189677E-4 5.705859301269599E-5 64-65 0.001009937096324719 2.2823437205078396E-5 0.0 2.0541093484570557E-4 5.705859301269599E-5 66-67 0.0011126425637475717 2.2823437205078396E-5 0.0 2.1682265344824477E-4 5.705859301269599E-5 68-69 0.0013066417799907383 2.2823437205078396E-5 0.0 2.3394023135205356E-4 6.276445231396559E-5 70-71 0.0016033464636567574 2.2823437205078396E-5 0.0 2.4535194995459273E-4 6.847031161523519E-5 72-73 0.0018486984136113502 2.2823437205078396E-5 0.0 2.7388124646094076E-4 6.847031161523519E-5 74-75 0.002094050363565943 2.2823437205078396E-5 0.0 2.8529296506347995E-4 6.847031161523519E-5 76-77 0.002533401529763702 2.2823437205078396E-5 0.0 2.9670468366601915E-4 6.847031161523519E-5 78-79 0.002978458555262731 3.4235155807617595E-5 0.0 3.024105429672888E-4 6.847031161523519E-5 80-81 0.003503397610979534 3.4235155807617595E-5 0.0 3.0811640226855835E-4 6.847031161523519E-5 82-83 0.004376394084073783 3.4235155807617595E-5 0.0 3.0811640226855835E-4 7.988203021777439E-5 84-85 0.005517565944327703 3.4235155807617595E-5 0.0 3.0811640226855835E-4 7.988203021777439E-5 86-87 0.00683561944292098 3.4235155807617595E-5 0.0 3.0811640226855835E-4 7.988203021777439E-5 88-89 0.008701435434436139 3.4235155807617595E-5 0.0 3.1952812087109755E-4 7.988203021777439E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 226455 0.0 91.32822 5 AAGCAGT 227670 0.0 91.16112 1 GGTATCA 227510 0.0 91.14711 8 GTATCAA 227640 0.0 91.08775 9 TGGTATC 227730 0.0 90.97984 7 GTGGTAT 228310 0.0 90.79655 6 AGCAGTG 230540 0.0 90.02161 2 GCAGTGG 230220 0.0 89.97702 3 CAGTGGT 230480 0.0 89.747734 4 TCAACGC 226895 0.0 46.2306 12-13 CAACGCA 226820 0.0 46.136 12-13 ATCAACG 226970 0.0 46.111008 10-11 AACGCAG 227880 0.0 46.04103 14-15 ACGCAGA 228160 0.0 45.90755 14-15 CGCAGAG 228625 0.0 45.889908 16-17 TATCAAC 228460 0.0 45.841446 10-11 AGAGTAC 223500 0.0 45.580185 18-19 CAGAGTA 228885 0.0 45.39221 18-19 GAGTACT 129015 0.0 45.29316 20-21 GCAGAGT 231530 0.0 45.117332 16-17 >>END_MODULE