FastQCFastQC Report
Sat 18 Jun 2016
SRR578551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8762922
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174090.19866660915160492No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105480.12037080781958348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG4450.021.3119833
CAACGCA69900.019.66869412-13
CGCGTAG2500.018.9633954-55
ATCAACG72650.018.92418110-11
GGTATCA90450.017.351768
CTCGCGT2600.017.32227752-53
AGTGGTA90500.017.289885
TCAACGC68300.017.24886110-11
GTATCAA91450.017.2124949
CGTACGC2900.017.16758520-21
AAGCAGT96000.017.1425931
ACGTACG2800.016.93401320-21
ACGCAGA83650.016.6056714-15
TGGTATC99350.015.8450777
AACGCAG75300.015.64538112-13
GTGGTAT102150.015.4107526
ACTCGCG3250.015.31654150-51
CGTAGCG3250.014.58726456-57
CGCTAGT3700.014.44520290-91
AGAGTAC99150.013.58032218-19
Produced by FastQC (version 0.11.5)