##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578551_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8762922 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.984739907533125 31.0 31.0 34.0 30.0 34.0 2 32.255843313451834 34.0 31.0 34.0 30.0 34.0 3 32.326009178217035 34.0 31.0 34.0 30.0 34.0 4 35.895689588472884 37.0 35.0 37.0 35.0 37.0 5 35.77551369280703 37.0 35.0 37.0 33.0 37.0 6 35.74948904029957 37.0 35.0 37.0 33.0 37.0 7 35.74333675456657 37.0 35.0 37.0 33.0 37.0 8 35.661331574102796 37.0 35.0 37.0 33.0 37.0 9 37.29752461564761 39.0 37.0 39.0 33.0 39.0 10-11 37.29365661362728 39.0 37.0 39.0 33.0 39.0 12-13 37.2874061300557 39.0 37.0 39.0 33.0 39.0 14-15 38.59921850268666 40.0 38.0 41.0 34.0 41.0 16-17 38.55284601414916 40.0 38.0 41.0 34.0 41.0 18-19 38.50290650766948 40.0 38.0 41.0 33.5 41.0 20-21 38.441810562732385 40.0 38.0 41.0 34.0 41.0 22-23 38.32412327760078 40.0 38.0 41.0 33.5 41.0 24-25 38.24196860362331 40.0 38.0 41.0 33.0 41.0 26-27 38.07417423092434 40.0 37.0 41.0 33.0 41.0 28-29 37.97936173573153 40.0 37.0 41.0 33.0 41.0 30-31 37.88175879004743 40.0 37.0 41.0 33.0 41.0 32-33 37.74851716128478 40.0 37.0 41.0 32.0 41.0 34-35 37.61841609454015 40.0 37.0 41.0 32.0 41.0 36-37 37.49284393949872 40.0 37.0 41.0 31.5 41.0 38-39 37.3670552470968 40.0 36.0 41.0 31.0 41.0 40-41 37.22432294844117 39.5 36.0 41.0 31.0 41.0 42-43 37.065077664733295 39.0 36.0 41.0 31.0 41.0 44-45 36.91290005776612 39.0 35.5 41.0 30.0 41.0 46-47 36.99650755763888 39.0 36.0 41.0 31.0 41.0 48-49 36.97865215506882 39.0 36.0 41.0 31.0 41.0 50-51 36.95674587768784 39.0 36.0 41.0 31.0 41.0 52-53 36.974628326030974 39.0 36.0 41.0 31.0 41.0 54-55 36.84780573192367 39.0 35.5 41.0 31.0 41.0 56-57 36.70596120791672 39.0 35.0 41.0 30.5 41.0 58-59 36.49537106458325 39.0 35.0 41.0 30.0 41.0 60-61 36.27242111706575 39.0 35.0 41.0 30.0 41.0 62-63 36.00553177353399 38.0 35.0 40.0 29.0 41.0 64-65 35.703214692542055 38.0 34.5 40.0 29.0 41.0 66-67 35.38944463958483 37.0 34.0 40.0 29.0 41.0 68-69 35.06071262530923 37.0 34.0 39.5 28.5 41.0 70-71 34.69595889362019 36.0 34.0 39.0 28.0 41.0 72-73 34.271498365499546 36.0 34.0 39.0 28.0 40.5 74-75 33.88298144157851 35.0 33.0 38.0 27.5 40.0 76-77 32.43979810615683 34.5 31.5 36.5 26.0 39.0 78-79 33.03888406173192 35.0 32.5 37.0 26.0 39.0 80-81 32.979371949219676 35.0 33.0 36.0 26.0 39.0 82-83 32.697947499703865 35.0 33.0 36.0 26.0 37.5 84-85 32.40709554415753 35.0 33.0 36.0 26.0 37.0 86-87 32.11798416099105 35.0 32.5 35.0 26.0 37.0 88-89 31.864098470806884 35.0 32.0 35.0 25.0 36.0 90-91 31.6201746974354 35.0 32.0 35.0 25.0 36.0 92-93 31.378187150359206 35.0 32.0 35.0 24.5 36.0 94-95 31.12548662421051 35.0 32.0 35.0 24.0 35.5 96-97 30.52268330130064 34.0 31.5 35.0 18.5 35.0 98-99 30.496099303405874 34.0 31.5 35.0 19.5 35.0 100-101 29.916748773982015 34.0 30.5 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43843178498370605 1101 2 0.4027340225290317 1101 3 0.38888558111654703 1101 4 0.2856370062895195 1101 5 0.286108041339304 1101 6 0.2633982573785758 1101 7 0.3100998100285679 1101 8 0.390638079953618 1101 9 0.4592033682916252 1101 10-11 0.43614782112098993 1101 12-13 0.4252362124388682 1101 14-15 0.5785843929249666 1101 16-17 0.5702350969870125 1101 18-19 0.5329274106875985 1101 20-21 0.5933277970591888 1101 22-23 0.6580180371734272 1101 24-25 0.6619352350488654 1101 26-27 0.6689300330165295 1101 28-29 0.6733106188120743 1101 30-31 0.642802856899209 1101 32-33 0.6019838594789846 1101 34-35 0.6206212831557849 1101 36-37 0.6471764058511482 1101 38-39 0.6858951404426463 1101 40-41 0.7215619953821957 1101 42-43 0.7182566112899735 1101 44-45 0.6974309849967284 1101 46-47 0.7105462579561106 1101 48-49 0.6751047403941541 1101 50-51 0.6440194667790919 1101 52-53 0.6057814480798385 1101 54-55 0.6876973891920528 1101 56-57 0.6609599787055203 1101 58-59 0.6791996625797978 1101 60-61 0.6260740136714276 1101 62-63 0.6375774518361013 1101 64-65 0.620136819144733 1101 66-67 0.5718409658235757 1101 68-69 0.6047391280017678 1101 70-71 0.5867347643652181 1101 72-73 0.5764122867183588 1101 74-75 0.6314688126808718 1101 76-77 0.5777357375865719 1101 78-79 0.5023576952573734 1101 80-81 0.4185827897035779 1101 82-83 0.4273842866898079 1101 84-85 0.34777742031949543 1101 86-87 0.39643781377029796 1101 88-89 0.4099561501778588 1101 90-91 0.4326254582299498 1101 92-93 0.5322263310610573 1101 94-95 0.5030966437003492 1101 96-97 0.8963218511890751 1101 98-99 0.6464370124441778 1101 100-101 0.6322181210483215 1102 1 0.4150854335411793 1102 2 0.39668750001400355 1102 3 0.3599137725880013 1102 4 0.2524128037151243 1102 5 0.2940036516275484 1102 6 0.3007754066281336 1102 7 0.2576907241790991 1102 8 0.295383670663 1102 9 0.4296034307138896 1102 10-11 0.4256405445668605 1102 12-13 0.4255119043206932 1102 14-15 0.5042611298279951 1102 16-17 0.5402466726996877 1102 18-19 0.5368079634540237 1102 20-21 0.571983546895666 1102 22-23 0.5910720909556844 1102 24-25 0.606378192409359 1102 26-27 0.6389461097364801 1102 28-29 0.64996125459853 1102 30-31 0.6930024961044126 1102 32-33 0.6827909099978484 1102 34-35 0.7547604959164076 1102 36-37 0.7869895928919348 1102 38-39 0.8195453526400698 1102 40-41 0.7842956711647489 1102 42-43 0.774517608703583 1102 44-45 0.7749449730954581 1102 46-47 0.8568452357472793 1102 48-49 0.8254960429988998 1102 50-51 0.8112948238129434 1102 52-53 0.7564325818740656 1102 54-55 0.7986316381422753 1102 56-57 0.732382811028792 1102 58-59 0.7989651471018391 1102 60-61 0.8790619608623587 1102 62-63 0.8355583366112782 1102 64-65 0.7991092362772179 1102 66-67 0.8064732936626484 1102 68-69 0.8417640661714216 1102 70-71 0.7829992702137503 1102 72-73 0.8328917433899434 1102 74-75 0.7068426910809364 1102 76-77 0.6839393207112607 1102 78-79 0.7047817206829023 1102 80-81 0.688265290965731 1102 82-83 0.7065991660325821 1102 84-85 0.7013032385726348 1102 86-87 0.717921161652562 1102 88-89 0.6981983458400158 1102 90-91 0.7046672703198524 1102 92-93 0.6845366063182929 1102 94-95 0.7169460198007584 1102 96-97 1.1022666098011804 1102 98-99 0.8903464711495488 1102 100-101 0.9185163292432996 1103 1 0.3303612996006535 1103 2 0.30400102891265135 1103 3 0.2799288532501052 1103 4 0.23081129241446519 1103 5 0.24640157444531496 1103 6 0.2990035330055676 1103 7 0.28912122881337865 1103 8 0.11871575670942036 1103 9 0.18618756876433906 1103 10-11 0.3700057517684954 1103 12-13 0.3646312393700555 1103 14-15 0.47941751165674873 1103 16-17 0.48273981901957086 1103 18-19 0.40208724015785435 1103 20-21 0.38120162488911546 1103 22-23 0.39885908508815504 1103 24-25 0.2550309255855865 1103 26-27 0.25033071162652476 1103 28-29 0.45622544560233536 1103 30-31 0.33717582089686005 1103 32-33 0.5093665683999333 1103 34-35 0.5040179505391933 1103 36-37 0.5608253442186921 1103 38-39 0.6014976905500191 1103 40-41 0.4744782801183547 1103 42-43 0.5910890821492387 1103 44-45 0.6677690768607221 1103 46-47 0.49522039814480223 1103 48-49 0.5025408663153925 1103 50-51 0.5217097840687828 1103 52-53 0.5736139366136257 1103 54-55 0.6259567925079281 1103 56-57 0.5534421106045073 1103 58-59 0.3119535102057185 1103 60-61 0.3312330282651459 1103 62-63 0.20386988282083252 1103 64-65 0.4428747090361469 1103 66-67 0.39436912252164547 1103 68-69 0.3717732629863164 1103 70-71 0.38643809241207094 1103 72-73 0.5865863535307838 1103 74-75 0.5283221192680116 1103 76-77 0.3087009189487162 1103 78-79 0.4692643911862149 1103 80-81 0.4534502972854426 1103 82-83 0.48952851520807883 1103 84-85 0.5335320466903752 1103 86-87 0.4379750443398791 1103 88-89 0.3664228097441402 1103 90-91 0.4587229190389479 1103 92-93 0.45882805651019964 1103 94-95 0.45761853890804005 1103 96-97 0.6000125664658817 1103 98-99 0.40602172493572297 1103 100-101 0.676949259622166 1104 1 0.34528240639232877 1104 2 0.32623976662132037 1104 3 0.29896612743259254 1104 4 0.21794826931819244 1104 5 0.2462300674706981 1104 6 0.27910872394999586 1104 7 0.2536192850676642 1104 8 0.3233235774274661 1104 9 0.33162548323952734 1104 10-11 0.35422711010374286 1104 12-13 0.33456034982054206 1104 14-15 0.42716505339011235 1104 16-17 0.46170163013321996 1104 18-19 0.4486227992705878 1104 20-21 0.4015823703727648 1104 22-23 0.544697182504116 1104 24-25 0.5016579550847879 1104 26-27 0.5648745030740443 1104 28-29 0.5505542816416664 1104 30-31 0.6081282562959061 1104 32-33 0.6148719422851343 1104 34-35 0.5982038640996805 1104 36-37 0.6203096799150174 1104 38-39 0.6245653786360066 1104 40-41 0.6060241296495619 1104 42-43 0.7018825877517969 1104 44-45 0.6709705403869037 1104 46-47 0.5093125545591661 1104 48-49 0.6653867387142043 1104 50-51 0.5776782267854585 1104 52-53 0.6161190818228661 1104 54-55 0.6778662368253308 1104 56-57 0.7235770294245398 1104 58-59 0.7374623142304202 1104 60-61 0.6523512537396456 1104 62-63 0.5068083689859364 1104 64-65 0.6794685805202505 1104 66-67 0.7208326487050485 1104 68-69 0.7439433979051415 1104 70-71 0.7084424372554281 1104 72-73 0.7957105245804073 1104 74-75 0.766802567472908 1104 76-77 0.6375941272725214 1104 78-79 0.6546634307471493 1104 80-81 0.7850876172031178 1104 82-83 0.8453483185467547 1104 84-85 0.8673417882865309 1104 86-87 0.7711273425333403 1104 88-89 0.7691211861447762 1104 90-91 0.7754391321649479 1104 92-93 0.7737720308984883 1104 94-95 0.8008325796123614 1104 96-97 1.1841653220112782 1104 98-99 0.9149686721115735 1104 100-101 0.9940084866973997 1105 1 0.14004200297293323 1105 2 0.13807958348061078 1105 3 0.11189569148623235 1105 4 0.0325900415444309 1105 5 0.0995586148534855 1105 6 0.04687850848225139 1105 7 0.07502183099342119 1105 8 0.17248296207668545 1105 9 0.14423297656356482 1105 10-11 0.11760808426335245 1105 12-13 0.14712135194610454 1105 14-15 0.13157786224324752 1105 16-17 0.14747895972276837 1105 18-19 0.06636906641659834 1105 20-21 0.13571160228393353 1105 22-23 0.14198335485581737 1105 24-25 0.1505633469641694 1105 26-27 0.1332035253030952 1105 28-29 0.1641618449430453 1105 30-31 0.1299231377030452 1105 32-33 0.15741404427961214 1105 34-35 0.1086638776702884 1105 36-37 0.1286510918828938 1105 38-39 0.1904512855736371 1105 40-41 0.24860621869823518 1105 42-43 0.1410869828288881 1105 44-45 0.24190380074387008 1105 46-47 0.21984428866024786 1105 48-49 0.1952964679393716 1105 50-51 0.13354474624978252 1105 52-53 0.23119548833625458 1105 54-55 0.28267346173627317 1105 56-57 0.22510542210741136 1105 58-59 0.21836364117582008 1105 60-61 0.21720390769292663 1105 62-63 0.2667784411750702 1105 64-65 0.204502407068901 1105 66-67 0.2312879825407137 1105 68-69 0.20786524695650144 1105 70-71 0.26654660103240957 1105 72-73 0.2989599538276977 1105 74-75 0.3084334707179863 1105 76-77 0.3120475479775635 1105 78-79 0.28190868509605593 1105 80-81 0.21045592921210243 1105 82-83 0.2652704250147764 1105 84-85 0.2946687161936197 1105 86-87 0.23557658334162568 1105 88-89 0.20397385259400735 1105 90-91 0.22582320157248148 1105 92-93 0.24916124863924693 1105 94-95 0.334342649477243 1105 96-97 0.6837240336283905 1105 98-99 0.4557039340705167 1105 100-101 0.4455508998411446 1106 1 -0.08063688642665312 1106 2 -0.10705924157088731 1106 3 -0.07888874240376964 1106 4 -0.08005265630892922 1106 5 -0.05242119550690205 1106 6 -0.08252090932072065 1106 7 -0.06315054699204126 1106 8 -0.04845536248964777 1106 9 -0.02302814391036634 1106 10-11 -0.11137945467745425 1106 12-13 -0.10778876500399548 1106 14-15 -0.16777278379117888 1106 16-17 -0.14587871693657917 1106 18-19 -0.15305912077165829 1106 20-21 -0.19673566802912745 1106 22-23 -0.13796736267648413 1106 24-25 -0.059798057798957416 1106 26-27 -0.0642796641833101 1106 28-29 -0.14727270896124622 1106 30-31 -0.2518780981888824 1106 32-33 -0.2779888973474769 1106 34-35 -0.3585469389376499 1106 36-37 -0.3021867100679785 1106 38-39 -0.3051214635198747 1106 40-41 -0.3265631631855612 1106 42-43 -0.2506259021105919 1106 44-45 -0.2284038954922778 1106 46-47 -0.2848440146269482 1106 48-49 -0.28466599567331485 1106 50-51 -0.2900839414236458 1106 52-53 -0.17820069503878955 1106 54-55 -0.21381335371408028 1106 56-57 -0.17804806000960838 1106 58-59 -0.16619928547076057 1106 60-61 -0.2787967221693535 1106 62-63 -0.19427402465064603 1106 64-65 -0.11174914630767319 1106 66-67 -0.1469438918953827 1106 68-69 -0.18848870159906994 1106 70-71 -0.14892307194990195 1106 72-73 -0.11495608723252104 1106 74-75 -0.16269304648406546 1106 76-77 -0.23837105509002754 1106 78-79 -0.1597225730964027 1106 80-81 -0.19209611954264005 1106 82-83 -0.22044855426785404 1106 84-85 -0.19637345053262578 1106 86-87 -0.23302682472401415 1106 88-89 -0.30107347219361813 1106 90-91 -0.24210335989016585 1106 92-93 -0.16464848188183723 1106 94-95 -0.1793627281847776 1106 96-97 0.11152377146949632 1106 98-99 -0.04091685478943674 1106 100-101 -0.001483385094932288 1107 1 -0.16404732835486158 1107 2 -0.2624588556977372 1107 3 -0.21817329434428956 1107 4 -0.1700176746974904 1107 5 -0.15163701267929497 1107 6 -0.13479913733973348 1107 7 -0.14051835944431446 1107 8 -0.1447471743671045 1107 9 -0.21909840418449988 1107 10-11 -0.2345681547595646 1107 12-13 -0.1977176425055731 1107 14-15 -0.23660930298353122 1107 16-17 -0.26268505744013027 1107 18-19 -0.2854781619878892 1107 20-21 -0.2822809745825623 1107 22-23 -0.22035246940267683 1107 24-25 -0.1672979102690917 1107 26-27 -0.17256132275653613 1107 28-29 -0.16772299003315538 1107 30-31 -0.2741604958264787 1107 32-33 -0.32569922331668266 1107 34-35 -0.32718610090092426 1107 36-37 -0.3301668631866903 1107 38-39 -0.36562086024587614 1107 40-41 -0.3383287933068573 1107 42-43 -0.3595477066686854 1107 44-45 -0.2747636077948812 1107 46-47 -0.21538544347704658 1107 48-49 -0.36780672784701807 1107 50-51 -0.29651859418235915 1107 52-53 -0.3181844389768216 1107 54-55 -0.3032874225752593 1107 56-57 -0.31617375566665373 1107 58-59 -0.3007932942802043 1107 60-61 -0.3609274433802483 1107 62-63 -0.5291397923861254 1107 64-65 -0.4886098097717593 1107 66-67 -0.35334274442222835 1107 68-69 -0.38621755920637924 1107 70-71 -0.35256546908856734 1107 72-73 -0.30732919923017477 1107 74-75 -0.32592600133384053 1107 76-77 -0.36776640484377765 1107 78-79 -0.19897980187650433 1107 80-81 -0.27547015330061697 1107 82-83 -0.27842598162158083 1107 84-85 -0.29405110767656595 1107 86-87 -0.2571129649768622 1107 88-89 -0.25971066202582804 1107 90-91 -0.26587921836463124 1107 92-93 -0.2780866237874342 1107 94-95 -0.25045959886402613 1107 96-97 0.04589084894526252 1107 98-99 -0.25160489321449475 1107 100-101 -0.29857571814326533 1108 1 -0.3429837748829243 1108 2 -0.3077035428759025 1108 3 -0.3167927064092879 1108 4 -0.18014034352001573 1108 5 -0.239820530700932 1108 6 -0.24179508584768428 1108 7 -0.2545604933578218 1108 8 -0.22684202855494817 1108 9 -0.30574669035339497 1108 10-11 -0.3615958932728418 1108 12-13 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-0.17257317605370304 2305 16-17 -0.13280055184990402 2305 18-19 -0.15160768824414106 2305 20-21 -0.15510799292683686 2305 22-23 -0.21981054666843391 2305 24-25 -0.15401860358286257 2305 26-27 -0.19339218943397185 2305 28-29 -0.1874736817892071 2305 30-31 -0.23674153473150739 2305 32-33 -0.2237847840805287 2305 34-35 -0.2231873395092876 2305 36-37 -0.1568034363487456 2305 38-39 -0.18053286654866696 2305 40-41 -0.19736022606737436 2305 42-43 -0.2379956561555545 2305 44-45 -0.24398592021462662 2305 46-47 -0.16746970432517827 2305 48-49 -0.18581354871816558 2305 50-51 -0.2040181952767668 2305 52-53 -0.18900152755178823 2305 54-55 -0.09603558920012034 2305 56-57 -0.17397960853111982 2305 58-59 -0.15144897792612966 2305 60-61 -0.181000475743204 2305 62-63 -0.1810899682795295 2305 64-65 -0.166684511452182 2305 66-67 -0.19441788303721097 2305 68-69 -0.15320905727728018 2305 70-71 -0.1492590160357139 2305 72-73 -0.15057545988857868 2305 74-75 -0.2003080634105885 2305 76-77 -0.1627932168617221 2305 78-79 -0.13864937512006748 2305 80-81 -0.11757179659693406 2305 82-83 -0.12600793332195082 2305 84-85 -0.09859327633484583 2305 86-87 -0.10062404837412942 2305 88-89 -0.09845225754514786 2305 90-91 -0.10360180290741994 2305 92-93 -0.06323175724687147 2305 94-95 -0.18473461505166355 2305 96-97 0.049313876206557694 2305 98-99 -0.19983843438779658 2305 100-101 -0.11055991450923486 2306 1 -0.2434908384408665 2306 2 -0.26195033249005206 2306 3 -0.25864294545490196 2306 4 -0.1887467993312768 2306 5 -0.21165505791871908 2306 6 -0.15327691727504344 2306 7 -0.23312695501272174 2306 8 -0.22953317173251264 2306 9 -0.35364410223379394 2306 10-11 -0.2478341201738843 2306 12-13 -0.2996930372814788 2306 14-15 -0.3596849775706801 2306 16-17 -0.40132864194530526 2306 18-19 -0.353875182758415 2306 20-21 -0.4428827567508975 2306 22-23 -0.4051253833866326 2306 24-25 -0.39863054938064835 2306 26-27 -0.44961598296558947 2306 28-29 -0.5320289618193712 2306 30-31 -0.5983630657975141 2306 32-33 -0.5688259129168571 2306 34-35 -0.5509188875419326 2306 36-37 -0.5675783305074802 2306 38-39 -0.7792216787259392 2306 40-41 -0.554727316695228 2306 42-43 -0.6651925975282111 2306 44-45 -0.6825631258883789 2306 46-47 -0.5553156322429018 2306 48-49 -0.6072201688057888 2306 50-51 -0.5996075882643623 2306 52-53 -0.5215621397103547 2306 54-55 -0.5600269362391472 2306 56-57 -0.555478506749715 2306 58-59 -0.5594209992651002 2306 60-61 -0.533695655691119 2306 62-63 -0.5236263350834776 2306 64-65 -0.5838998619832836 2306 66-67 -0.5608394504154575 2306 68-69 -0.5389321110934873 2306 70-71 -0.5711169140498598 2306 72-73 -0.6104836971386263 2306 74-75 -0.5848166470581617 2306 76-77 -0.5798658341048473 2306 78-79 -0.45974239526131555 2306 80-81 -0.5028413792916382 2306 82-83 -0.5001487085846037 2306 84-85 -0.4419668415172424 2306 86-87 -0.44329222870536356 2306 88-89 -0.43951549053342376 2306 90-91 -0.39410622961995756 2306 92-93 -0.4398028223021271 2306 94-95 -0.4191619007507512 2306 96-97 -0.20949263350882674 2306 98-99 -0.4925338774134751 2306 100-101 -0.49925693544165384 2307 1 -0.47504884014345095 2307 2 -0.45047986281410246 2307 3 -0.45161787124468944 2307 4 -0.36760611861571135 2307 5 -0.37323701140354615 2307 6 -0.3727118240673519 2307 7 -0.3794675954856359 2307 8 -0.37924228150480843 2307 9 -0.4370516418198065 2307 10-11 -0.5810663171190242 2307 12-13 -0.5362901311525761 2307 14-15 -0.8906685573819431 2307 16-17 -0.7769656016281985 2307 18-19 -0.7021104427918132 2307 20-21 -0.6038594811563058 2307 22-23 -0.6097868280444416 2307 24-25 -0.5674029515818333 2307 26-27 -0.683508977715114 2307 28-29 -0.6993121269427292 2307 30-31 -0.694467520745988 2307 32-33 -0.7444751539619361 2307 34-35 -0.7665732829579284 2307 36-37 -0.8200898315617664 2307 38-39 -0.8267411974265855 2307 40-41 -0.7160358854573872 2307 42-43 -0.87075154317764 2307 44-45 -0.8104299480572763 2307 46-47 -0.7625825087438827 2307 48-49 -0.7073925867769333 2307 50-51 -0.7839441487542231 2307 52-53 -0.8433260964306442 2307 54-55 -0.8465327789140176 2307 56-57 -0.8742435816466383 2307 58-59 -0.8420974201825686 2307 60-61 -0.9500306751463583 2307 62-63 -0.9253721240904795 2307 64-65 -0.8549354433977499 2307 66-67 -0.8385433213402607 2307 68-69 -0.8039190928745796 2307 70-71 -0.9430617126404002 2307 72-73 -1.2425894510956041 2307 74-75 -0.9442767957884257 2307 76-77 -0.8844438937901664 2307 78-79 -0.8556554325137 2307 80-81 -0.7993909479672467 2307 82-83 -0.8358207377959914 2307 84-85 -0.901373568447692 2307 86-87 -0.8156046941679413 2307 88-89 -0.8632397949840644 2307 90-91 -0.9041510947289133 2307 92-93 -0.8592304255476542 2307 94-95 -0.881911577705413 2307 96-97 -0.6724703284194327 2307 98-99 -0.9949301197217757 2307 100-101 -1.0666787729239964 2308 1 -1.423168191968017 2308 2 -0.8889737660458117 2308 3 -0.87841929050267 2308 4 -0.545666766245624 2308 5 -0.6477493663447262 2308 6 -0.6202051549927887 2308 7 -0.5850618250611674 2308 8 -0.6021512409069416 2308 9 -0.849009159330322 2308 10-11 -0.8210928160032083 2308 12-13 -0.7399887627164716 2308 14-15 -0.9804891684106707 2308 16-17 -0.9126847685796022 2308 18-19 -0.9024619722085419 2308 20-21 -0.9579004132528581 2308 22-23 -1.015796236353843 2308 24-25 -1.1645426542907202 2308 26-27 -1.227310561338733 2308 28-29 -1.2597782730532359 2308 30-31 -1.1528343542807988 2308 32-33 -1.1318557317242153 2308 34-35 -1.2302996243905469 2308 36-37 -1.2964666433161156 2308 38-39 -1.3069315501904484 2308 40-41 -1.2666391758764206 2308 42-43 -1.1815680820877574 2308 44-45 -1.1743105491043835 2308 46-47 -1.187579568499764 2308 48-49 -1.3157301978918028 2308 50-51 -1.310742623112354 2308 52-53 -1.3232559844736684 2308 54-55 -1.207009228449401 2308 56-57 -1.1202460135727463 2308 58-59 -1.135525336117304 2308 60-61 -1.0932298057602807 2308 62-63 -1.089986314338681 2308 64-65 -1.0493422343566579 2308 66-67 -1.0370618778035023 2308 68-69 -1.1419878032231097 2308 70-71 -1.0996981108600323 2308 72-73 -1.1348521873622062 2308 74-75 -1.1688677523660118 2308 76-77 -1.1307011576333181 2308 78-79 -1.5133649388279409 2308 80-81 -1.1963593486332371 2308 82-83 -1.1435070145366488 2308 84-85 -1.038452720440194 2308 86-87 -1.0384972430784494 2308 88-89 -1.0771368568046036 2308 90-91 -1.1509767763860097 2308 92-93 -1.1320325671503362 2308 94-95 -1.193086509188383 2308 96-97 -1.0251424954025055 2308 98-99 -1.3200916655424138 2308 100-101 -1.3194299704907344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44.0 3 87.0 4 187.0 5 437.0 6 968.0 7 1785.0 8 3548.0 9 5470.0 10 7436.0 11 9236.0 12 11028.0 13 12402.0 14 14283.0 15 16104.0 16 18135.0 17 21401.0 18 25024.0 19 28428.0 20 30937.0 21 31468.0 22 34167.0 23 40087.0 24 48678.0 25 61837.0 26 78526.0 27 100961.0 28 128594.0 29 160638.0 30 199414.0 31 246315.0 32 303526.0 33 381125.0 34 500698.0 35 731212.0 36 1018595.0 37 1735499.0 38 2122080.0 39 630297.0 40 2265.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.72216765500166 30.946633106114056 28.65877779114377 22.67242144774052 2 22.722726927200814 29.298746058534007 27.770474222304504 20.208052791960675 3 21.759779421289018 28.14842500432244 28.077439926595492 22.014355647793042 4 21.896660627933183 28.306120993395435 28.16292598034321 21.634292398328174 5 22.372293860491997 28.337566353967862 28.298378199243807 20.991761586296334 6 22.268724975527572 28.318556298914903 27.61856147983515 21.794157245722374 7 22.325007571675293 28.40907405087025 28.1113309008114 21.154587476643062 8 21.720732751295934 28.436790311666844 28.054968786980545 21.78750815005668 9 21.395880784874795 28.240452617658807 28.626939324075106 21.736727273391296 10-11 21.505714646324595 28.68007954424335 28.032144985428374 21.782060824003683 12-13 21.48591531454919 28.70493997321898 27.90499561675889 21.90414909547295 14-15 21.614633794526526 28.75514012335155 27.762611603755005 21.86761447836692 16-17 21.815485747790518 28.521314009185524 27.736718414245843 21.92648182877812 18-19 21.79095055279506 28.531835613737062 27.73078432057252 21.946429512895357 20-21 21.90721314191773 28.456518270960302 27.766400294331046 21.86986829279092 22-23 21.818086685875265 28.445230357407876 27.817294369664978 21.919388587051877 24-25 21.838279239746562 28.413903385324208 27.7071814787083 22.04063589622093 26-27 21.98667271376863 28.302383020760246 27.854893290866666 21.856050974604454 28-29 21.992846678311185 28.334989173702564 27.785589099161218 21.886575048825037 30-31 21.971090236795444 28.352420573867942 27.73377989670569 21.94270929263093 32-33 22.003128636772075 28.37033697207393 27.677605711884688 21.94892867926931 34-35 21.998946241904243 28.402341136894748 27.68412750906604 21.914585112134972 36-37 22.01754734322638 28.3926754112384 27.645430371284828 21.944346874250392 38-39 22.03623973829734 28.377109827064533 27.648431653277296 21.93821878136083 40-41 22.02281385136145 28.43119566738127 27.629020319934376 21.9169701613229 42-43 22.048718452589217 28.410614632881586 27.589729772785837 21.95093714174336 44-45 22.04450182256558 28.427498270554047 27.576007181166283 21.951992725714096 46-47 22.027053304822296 28.4560446846383 27.557314786095322 21.959587224444082 48-49 22.070075483953868 28.428171561951594 27.52209251662859 21.97966043746595 50-51 22.07178153588495 28.436713233325595 27.514851781175274 21.97665344961418 52-53 22.039320902320025 28.495300996631034 27.484781902657584 21.980596198391357 54-55 22.09520408831666 28.491757658004946 27.397025786604058 22.01601246707434 56-57 22.087056121234447 28.508298944119325 27.405944044691942 21.998700889954286 58-59 22.103597407348825 28.579816184601437 27.361968987057057 21.95461742099268 60-61 22.087827007882478 28.543820355761568 27.38357918271416 21.984773453641793 62-63 22.07748732086089 28.567928432511202 27.367580489032335 21.987003757595573 64-65 22.052464249708187 28.647061552279407 27.349260670025867 21.95121352798654 66-67 22.069582026380623 28.636738745276325 27.328514576123375 21.965164652219677 68-69 22.03489586581521 28.652950013525775 27.35194813635052 21.960205984308505 70-71 22.044206192861107 28.707584379052054 27.32340988227373 21.924799545813105 72-73 22.02963167528632 28.701443126263847 27.29014985872289 21.97877533972694 74-75 22.093826389004985 28.72493907138358 27.274440587345417 21.906793952266018 76-77 22.043224495613767 28.768692304901766 27.272091061599806 21.91599213788466 78-79 22.058482670426972 28.760074901663025 27.2438585774393 21.937583850470705 80-81 22.03163182029636 28.814550498113274 27.21215590722564 21.941661774364736 82-83 22.02835111709276 28.845774946567698 27.21225883710698 21.913615099232562 84-85 22.014908211202968 28.858771901492545 27.179438250348063 21.946881636956423 86-87 22.01057067030009 28.92004830223825 27.151689170218436 21.917691857243224 88-89 22.018060282490364 28.961081544141415 27.126515048111944 21.89434312525628 90-91 22.01615282424717 28.92685333175928 27.158432154018275 21.898561689975278 92-93 21.98622336556102 28.990535342088254 27.126545308723475 21.896695983627254 94-95 21.987441717659994 29.047509835702705 27.09393006833225 21.871118378305045 96-97 21.97551831194041 29.045562795116325 27.096911360329436 21.882007532613834 98-99 21.993331377807145 29.073019488700897 27.04488149585534 21.88876763763662 100-101 22.0015269984215 29.110891123253964 27.005667091733756 21.88191478659078 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 107884.0 1 59818.0 2 8619.0 3 4943.0 4 4252.5 5 3889.0 6 3607.5 7 3494.0 8 3237.0 9 3049.0 10 3127.5 11 3611.0 12 7103.0 13 7072.5 14 4005.5 15 4336.0 16 4942.0 17 5716.5 18 7161.0 19 9199.0 20 12043.5 21 16089.5 22 21234.5 23 27739.5 24 36083.5 25 46211.5 26 59251.5 27 73995.5 28 85645.0 29 96855.5 30 112356.5 31 129074.0 32 145581.5 33 164471.5 34 185215.5 35 204202.5 36 222393.0 37 238833.5 38 250733.0 39 257778.0 40 267428.5 41 279388.5 42 287940.5 43 298744.5 44 311276.5 45 323112.5 46 330346.5 47 332657.0 48 335777.5 49 338443.5 50 338565.0 51 331468.5 52 318658.0 53 305503.0 54 286435.5 55 263584.5 56 239361.5 57 213239.5 58 187071.5 59 156093.5 60 122615.0 61 93604.5 62 69703.5 63 50285.0 64 35301.0 65 23364.5 66 14247.0 67 8662.5 68 4801.5 69 2224.0 70 997.0 71 462.0 72 231.0 73 126.0 74 90.5 75 55.0 76 35.5 77 31.5 78 19.0 79 13.5 80 12.5 81 10.5 82 8.0 83 4.5 84 1.5 85 1.0 86 1.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011959481095461081 2 0.018121809140832248 3 0.005899858517512766 4 5.363507743193424E-4 5 7.98820302177744E-4 6 0.0 7 0.0 8 0.008136555363610447 9 0.010418899084118288 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.417617091650479E-5 24-25 0.004827156968874081 26-27 0.006824207724318441 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 2.8529296506348E-5 62-63 5.135273371142639E-5 64-65 2.4535194995459273E-4 66-67 1.4264648253173998E-4 68-69 2.4535194995459273E-4 70-71 1.0841132672412238E-4 72-73 2.2823437205078396E-5 74-75 3.9941015108887194E-5 76-77 0.12352614801318555 78-79 0.05445672117131706 80-81 6.276445231396559E-5 82-83 0.002265226142604031 84-85 0.010327605335297975 86-87 0.11546947467979288 88-89 0.13133176353732237 90-91 0.14478617976971608 92-93 0.14294889307470726 94-95 0.11108737473641782 96-97 1.2115365171571766 98-99 0.07463834551990764 100-101 0.06702102335271272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8762922.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.32393660671339 #Duplication Level Percentage of deduplicated Percentage of total 1 89.71034883171518 68.47046977197974 2 6.42207601299472 9.803162449986072 3 1.4860098535895727 3.40254365586966 4 0.6295589009975985 1.922016545997314 5 0.3439599745460901 1.3126189646251265 6 0.23437127722246526 1.0732883103097133 7 0.17598350087412798 0.9402227495181104 8 0.13128859986454222 0.8016370218596384 9 0.1035628147290067 0.7113889535570561 >10 0.7558154181992836 9.926056278919889 >50 0.00607639355502214 0.2785057384439566 >100 6.738507019341963E-4 0.09777524811243751 >500 3.9224430182853324E-5 0.01826195382853437 >1k 1.17673290133461E-4 0.24474011806825327 >5k 3.922443004448695E-5 0.16034594157474788 >10k+ 7.84488600889739E-5 0.8369662973497436 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17409 0.19866660915160492 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10548 0.12037080781958348 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.4235155807617595E-5 0.0 0.0 0.0 0.0 2 3.4235155807617595E-5 0.0 0.0 0.0 0.0 3 4.564687441015679E-5 0.0 0.0 0.0 0.0 4 4.564687441015679E-5 0.0 0.0 2.2823437205078396E-5 0.0 5 4.564687441015679E-5 0.0 0.0 4.564687441015679E-5 2.2823437205078396E-5 6 4.564687441015679E-5 0.0 0.0 4.564687441015679E-5 2.2823437205078396E-5 7 4.564687441015679E-5 0.0 0.0 4.564687441015679E-5 2.2823437205078396E-5 8 4.564687441015679E-5 0.0 0.0 5.705859301269599E-5 5.705859301269599E-5 9 5.705859301269599E-5 0.0 0.0 5.705859301269599E-5 5.705859301269599E-5 10-11 6.847031161523519E-5 0.0 0.0 5.705859301269599E-5 5.705859301269599E-5 12-13 8.558788951904399E-5 0.0 0.0 5.705859301269599E-5 7.417617091650479E-5 14-15 9.699960812158318E-5 0.0 0.0 5.705859301269599E-5 1.0270546742285278E-4 16-17 1.0841132672412238E-4 0.0 0.0 6.276445231396559E-5 1.0270546742285278E-4 18-19 1.1982304532666158E-4 0.0 0.0 7.988203021777439E-5 1.0841132672412238E-4 20-21 1.4835234183300958E-4 0.0 0.0 9.129374882031359E-5 1.1411718602539198E-4 22-23 1.6546991973681837E-4 0.0 0.0 9.699960812158318E-5 1.1411718602539198E-4 24-25 1.8829335694189677E-4 0.0 0.0 1.0270546742285278E-4 1.2552890462793118E-4 26-27 1.9399921624316637E-4 0.0 0.0 1.0270546742285278E-4 1.3123476392920078E-4 28-29 2.2252851274951436E-4 0.0 0.0 1.0270546742285278E-4 1.4835234183300958E-4 30-31 2.5105780925586236E-4 0.0 0.0 1.1411718602539198E-4 1.4835234183300958E-4 32-33 2.6246952785840156E-4 0.0 0.0 1.1411718602539198E-4 1.5976406043554877E-4 34-35 2.6246952785840156E-4 0.0 0.0 1.2552890462793118E-4 1.7117577903808797E-4 36-37 2.795871057622104E-4 0.0 0.0 1.3694062323047038E-4 1.8258749764062717E-4 38-39 2.9670468366601915E-4 0.0 0.0 1.3694062323047038E-4 1.8258749764062717E-4 40-41 3.252339801723672E-4 0.0 0.0 1.3694062323047038E-4 1.8258749764062717E-4 42-43 3.4235155807617595E-4 0.0 0.0 1.5976406043554877E-4 1.8829335694189677E-4 44-45 3.9941015108887194E-4 0.0 0.0 1.7117577903808797E-4 2.0541093484570557E-4 46-47 4.3364530689648953E-4 0.0 0.0 1.7117577903808797E-4 2.2252851274951436E-4 48-49 4.8499804060791595E-4 0.0 0.0 1.7117577903808797E-4 2.2823437205078396E-4 50-51 5.306449150180727E-4 0.0 0.0 1.7117577903808797E-4 2.2823437205078396E-4 52-53 5.705859301269599E-4 0.0 0.0 1.7688163833935757E-4 2.3964609065332316E-4 54-55 6.56173819646004E-4 0.0 0.0 1.8258749764062717E-4 2.5105780925586236E-4 56-57 7.474675684663176E-4 5.705859301269599E-6 0.0 2.0541093484570557E-4 2.5105780925586236E-4 58-59 8.387613172866311E-4 2.2823437205078396E-5 0.0 2.0541093484570557E-4 2.6246952785840156E-4 60-61 0.001009937096324719 2.2823437205078396E-5 0.0 2.0541093484570557E-4 2.7388124646094076E-4 62-63 0.0011582894381577287 2.2823437205078396E-5 0.0 2.1111679414697517E-4 2.7388124646094076E-4 64-65 0.001312347639292008 2.2823437205078396E-5 0.0 2.1682265344824477E-4 2.8529296506347995E-4 66-67 0.0014150531067148608 2.2823437205078396E-5 0.0 2.1682265344824477E-4 2.9670468366601915E-4 68-69 0.001609052322958027 2.2823437205078396E-5 0.0 2.3964609065332316E-4 3.366456987749063E-4 70-71 0.0019171687252265853 2.2823437205078396E-5 0.0 2.4535194995459273E-4 3.594691359799847E-4 72-73 0.002196755830988796 2.2823437205078396E-5 0.0 2.7388124646094076E-4 3.7088085458252397E-4 74-75 0.002447813640244658 2.2823437205078396E-5 0.0 2.7388124646094076E-4 3.7658671388379354E-4 76-77 0.002909988243647496 2.2823437205078396E-5 0.0 2.9670468366601915E-4 3.8799843248633274E-4 78-79 0.0033949862842554118 2.2823437205078396E-5 0.0 2.9670468366601915E-4 4.1082186969141113E-4 80-81 0.003919925339972215 2.2823437205078396E-5 0.0 3.252339801723672E-4 4.1082186969141113E-4 82-83 0.004764392516560115 2.2823437205078396E-5 0.0 3.4235155807617595E-4 4.3364530689648953E-4 84-85 0.005882740939608957 2.2823437205078396E-5 0.0 3.6517499528125434E-4 4.3364530689648953E-4 86-87 0.007155147563792078 2.2823437205078396E-5 0.0 3.822925731850631E-4 4.3364530689648953E-4 88-89 0.008935375665788193 2.2823437205078396E-5 0.0 3.9941015108887194E-4 4.3364530689648953E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 445 0.0 21.311983 3 CAACGCA 6990 0.0 19.668694 12-13 CGCGTAG 250 0.0 18.96339 54-55 ATCAACG 7265 0.0 18.924181 10-11 GGTATCA 9045 0.0 17.35176 8 CTCGCGT 260 0.0 17.322277 52-53 AGTGGTA 9050 0.0 17.28988 5 TCAACGC 6830 0.0 17.248861 10-11 GTATCAA 9145 0.0 17.212494 9 CGTACGC 290 0.0 17.167585 20-21 AAGCAGT 9600 0.0 17.142593 1 ACGTACG 280 0.0 16.934013 20-21 ACGCAGA 8365 0.0 16.60567 14-15 TGGTATC 9935 0.0 15.845077 7 AACGCAG 7530 0.0 15.645381 12-13 GTGGTAT 10215 0.0 15.410752 6 ACTCGCG 325 0.0 15.316541 50-51 CGTAGCG 325 0.0 14.587264 56-57 CGCTAGT 370 0.0 14.445202 90-91 AGAGTAC 9915 0.0 13.580322 18-19 >>END_MODULE