##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578550_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7178701 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.752056952922263 31.0 31.0 34.0 27.0 34.0 2 31.055480928931292 31.0 31.0 34.0 27.0 34.0 3 31.149319633175974 33.0 31.0 34.0 27.0 34.0 4 34.557164311481976 37.0 35.0 37.0 30.0 37.0 5 34.642325958415036 37.0 35.0 37.0 32.0 37.0 6 34.64161413046734 37.0 35.0 37.0 31.0 37.0 7 34.59380492375988 37.0 35.0 37.0 32.0 37.0 8 34.57561625703592 37.0 35.0 37.0 31.0 37.0 9 35.99675818786714 39.0 35.0 39.0 30.0 39.0 10-11 35.93902267276489 39.0 35.0 39.0 30.5 39.0 12-13 36.02886316061917 39.0 35.0 39.0 30.5 39.0 14-15 37.13922330794945 40.0 37.0 41.0 31.0 41.0 16-17 37.094210150276496 40.0 37.0 41.0 31.0 41.0 18-19 37.07494782133982 40.0 37.0 41.0 31.0 41.0 20-21 36.93945589877612 40.0 36.5 41.0 31.0 41.0 22-23 36.6263722364255 39.5 36.5 40.5 30.5 41.0 24-25 36.542020485321785 39.0 36.0 40.5 30.0 41.0 26-27 36.49176863613626 39.0 36.0 41.0 30.0 41.0 28-29 36.26793782050541 39.0 36.0 41.0 29.0 41.0 30-31 36.03708567887143 39.0 35.5 41.0 27.5 41.0 32-33 35.63242479384502 39.0 35.0 40.5 26.5 41.0 34-35 35.42450876558308 38.0 34.0 40.0 25.5 41.0 36-37 35.065499593868026 38.0 34.0 40.0 25.0 41.0 38-39 34.20681492375849 36.0 33.0 39.5 25.0 41.0 40-41 34.34453120418304 37.0 33.0 40.0 25.0 41.0 42-43 34.250500543204126 37.0 33.0 40.0 24.0 41.0 44-45 34.10642461637558 37.0 33.0 40.0 23.5 41.0 46-47 33.87864538445048 37.0 33.0 40.0 23.0 41.0 48-49 33.471351153920466 36.0 33.0 40.0 21.0 41.0 50-51 32.15356009395015 35.5 31.5 39.0 15.5 40.5 52-53 31.59557279234781 35.5 31.0 39.0 5.0 40.0 54-55 30.976850129292195 36.0 29.5 40.0 2.0 41.0 56-57 30.327892957235576 36.0 28.0 40.0 2.0 41.0 58-59 29.933229702699695 36.0 28.0 40.0 2.0 41.0 60-61 29.62156586546786 35.0 27.0 39.0 2.0 41.0 62-63 29.294000404808614 35.0 26.0 39.0 2.0 41.0 64-65 28.968877725928408 35.0 26.0 39.0 2.0 41.0 66-67 28.653358455798617 35.0 26.0 38.5 2.0 40.0 68-69 28.332983223566494 35.0 26.0 38.0 2.0 40.0 70-71 27.979067382803656 35.0 25.0 37.0 2.0 40.0 72-73 27.59799391282629 34.0 24.0 36.5 2.0 39.0 74-75 27.233995189380362 34.0 23.5 36.0 2.0 39.0 76-77 26.85779829804863 34.0 22.0 36.0 2.0 38.5 78-79 26.511693271526422 34.0 20.5 35.0 2.0 37.5 80-81 26.166381146115434 34.0 20.0 35.0 2.0 37.0 82-83 25.80344730892121 33.0 18.5 35.0 2.0 36.5 84-85 25.475432532988904 33.0 17.5 35.0 2.0 36.0 86-87 25.16397388608329 33.0 13.0 35.0 2.0 36.0 88-89 24.823408023262147 33.0 7.0 35.0 2.0 35.0 90-91 24.48560958312653 32.0 4.0 35.0 2.0 35.0 92-93 24.154119248036658 32.0 2.0 35.0 2.0 35.0 94-95 23.743297290136475 32.0 2.0 35.0 2.0 35.0 96-97 23.280645272731096 32.0 2.0 35.0 2.0 35.0 98-99 22.78638656213708 31.0 2.0 35.0 2.0 35.0 100-101 21.498811275187528 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5338261289284354 1101 2 0.6283917306666034 1101 3 0.6156738443176835 1101 4 0.6725889192763077 1101 5 0.6929648078834276 1101 6 0.5683783449530324 1101 7 0.47587426047327597 1101 8 0.5068775347417755 1101 9 0.6973148104190869 1101 10-11 0.762892600080697 1101 12-13 0.7508139024909113 1101 14-15 0.8256466251051151 1101 16-17 0.7464391323835429 1101 18-19 0.7636265704640337 1101 20-21 0.7971117200866829 1101 22-23 0.7916690508579407 1101 24-25 0.8730860272980934 1101 26-27 1.0101362838382641 1101 28-29 0.9680827854204352 1101 30-31 0.9545944668071087 1101 32-33 0.9202918420613884 1101 34-35 1.0646288823676642 1101 36-37 1.090744995914676 1101 38-39 0.9952709676333669 1101 40-41 1.0291401491613712 1101 42-43 1.2420200369584293 1101 44-45 1.2168240588347814 1101 46-47 1.2927144020422006 1101 48-49 1.1368149265383423 1101 50-51 1.1487522982851104 1101 52-53 1.1105420487488686 1101 54-55 0.9784690839933994 1101 56-57 0.9529343998726105 1101 58-59 0.9023733526481017 1101 60-61 1.0565886921414176 1101 62-63 1.0936877916659533 1101 64-65 0.9769010303108594 1101 66-67 0.9967665969945259 1101 68-69 1.088063523482269 1101 70-71 1.105463094519667 1101 72-73 1.1632938012991154 1101 74-75 1.1153950614029036 1101 76-77 1.1531070874754832 1101 78-79 1.0989472332229369 1101 80-81 1.1770884806542092 1101 82-83 1.1875540064232197 1101 84-85 1.1858898855236504 1101 86-87 1.1762105272108165 1101 88-89 1.2259387407127846 1101 90-91 1.2205980420774907 1101 92-93 1.4658348427215344 1101 94-95 1.50260996216495 1101 96-97 1.5768603294083654 1101 98-99 1.6975120947802331 1101 100-101 1.9657921293769292 1102 1 0.4847272903312714 1102 2 0.45917039213024324 1102 3 0.5029069857503607 1102 4 0.676291286791745 1102 5 0.5116626758974476 1102 6 0.40494720564083764 1102 7 0.37046979928594226 1102 8 0.5226871192052371 1102 9 0.6334229413799548 1102 10-11 0.688455653303933 1102 12-13 0.5877571210952155 1102 14-15 0.6422126722908814 1102 16-17 0.6160658539270187 1102 18-19 0.6417988165424688 1102 20-21 0.7669036560553479 1102 22-23 0.7328903539627944 1102 24-25 0.724491644813753 1102 26-27 0.9127065020333021 1102 28-29 0.8054901042490741 1102 30-31 0.9680214324076175 1102 32-33 0.8856320190808162 1102 34-35 0.8705779378627128 1102 36-37 0.8879932512881794 1102 38-39 0.8939230764537385 1102 40-41 1.0007174353419757 1102 42-43 1.0740431551174083 1102 44-45 0.9438152721207516 1102 46-47 1.0253390751640055 1102 48-49 0.8980786934062266 1102 50-51 0.9525508413294403 1102 52-53 1.0610207344440568 1102 54-55 1.037416643558121 1102 56-57 1.090032533743063 1102 58-59 0.8756903109190937 1102 60-61 1.0623371542487696 1102 62-63 1.1402063981627109 1102 64-65 1.034909955147718 1102 66-67 1.081253406113877 1102 68-69 1.1130053167189935 1102 70-71 1.067042689753709 1102 72-73 1.1751465419413698 1102 74-75 1.215086304325233 1102 76-77 1.0664219859146655 1102 78-79 1.1086913111314196 1102 80-81 1.1295139476219838 1102 82-83 1.1912005618049228 1102 84-85 1.1538069166394216 1102 86-87 1.206356173233825 1102 88-89 1.2590696790562497 1102 90-91 1.3339173169085967 1102 92-93 1.4447124460741954 1102 94-95 1.4565210002511222 1102 96-97 1.5728156199482477 1102 98-99 1.7445794392696854 1102 100-101 1.9371945850807855 1103 1 0.3480206126716645 1103 2 0.4454279760570756 1103 3 0.3600208300383301 1103 4 0.4394575692331131 1103 5 0.4456307740903256 1103 6 0.3785113869422787 1103 7 0.32645698266227186 1103 8 0.39469066895217964 1103 9 0.3763114857303549 1103 10-11 0.457486442295334 1103 12-13 0.45817987888259637 1103 14-15 0.5778633488996334 1103 16-17 0.5491297893435743 1103 18-19 0.5685684298081668 1103 20-21 0.5945617164913912 1103 22-23 0.4557983655301925 1103 24-25 0.6116999551058271 1103 26-27 0.7064062788683572 1103 28-29 0.6469822628656559 1103 30-31 0.6890941858746515 1103 32-33 0.7025734423125414 1103 34-35 0.7582419561498028 1103 36-37 0.7781955321814991 1103 38-39 0.6991803803563741 1103 40-41 0.6022359499640473 1103 42-43 0.7580130271435976 1103 44-45 0.683057781027621 1103 46-47 0.6248921638963125 1103 48-49 0.8030274171786616 1103 50-51 0.7741337176824459 1103 52-53 0.6174199319358564 1103 54-55 0.6275971499509403 1103 56-57 0.6079691926256672 1103 58-59 0.5799562073074647 1103 60-61 0.6467448733472736 1103 62-63 0.7296801387800613 1103 64-65 0.6508051101941241 1103 66-67 0.7268939999197173 1103 68-69 0.6937891129243816 1103 70-71 0.5752196155664642 1103 72-73 0.6465833955927316 1103 74-75 0.6333132384926543 1103 76-77 0.6262985096560598 1103 78-79 0.573362021543673 1103 80-81 0.7073314208794486 1103 82-83 0.7234304816588626 1103 84-85 0.7336155688745514 1103 86-87 0.7788768223253122 1103 88-89 0.7803849703315713 1103 90-91 0.7846862262875192 1103 92-93 0.8456420257459101 1103 94-95 0.8224961635385135 1103 96-97 0.9515083415702357 1103 98-99 1.102300036710119 1103 100-101 1.3608882134938014 1104 1 0.30465921370439375 1104 2 0.25230155802859144 1104 3 0.30985382371725123 1104 4 0.40110958533053065 1104 5 0.29222768613803396 1104 6 0.2778078163013262 1104 7 0.34152800613346557 1104 8 0.3508272505909815 1104 9 0.4322363259429878 1104 10-11 0.47057073617878586 1104 12-13 0.41862492730289347 1104 14-15 0.44526336952053924 1104 16-17 0.5032466217154621 1104 18-19 0.4743843324136776 1104 20-21 0.4929903405733853 1104 22-23 0.3376213437499942 1104 24-25 0.4846132234516247 1104 26-27 0.5404384102963036 1104 28-29 0.49732108632201033 1104 30-31 0.5986444009891159 1104 32-33 0.5740007157584586 1104 34-35 0.6843356045521034 1104 36-37 0.6501139186869054 1104 38-39 0.6217583349534479 1104 40-41 0.6733650677339753 1104 42-43 0.714499267504344 1104 44-45 0.7779731495124835 1104 46-47 0.7466959928889239 1104 48-49 0.7668104326210354 1104 50-51 0.7319509135222759 1104 52-53 0.7186719627281306 1104 54-55 0.7786011176699397 1104 56-57 0.6100425442029191 1104 58-59 0.622172140483503 1104 60-61 0.582644464439003 1104 62-63 0.5442825397480853 1104 64-65 0.6887429995215619 1104 66-67 0.5323729377925339 1104 68-69 0.5181801267170947 1104 70-71 0.5299906366650582 1104 72-73 0.5645712867734254 1104 74-75 0.6422934431949834 1104 76-77 0.73979892803289 1104 78-79 0.6788842786114984 1104 80-81 0.5863719991130729 1104 82-83 0.5619017918764513 1104 84-85 0.6438329837813619 1104 86-87 0.7059648332187471 1104 88-89 0.7962029273734039 1104 90-91 0.7719137059642875 1104 92-93 0.8661643303751951 1104 94-95 0.8736300615083614 1104 96-97 0.8564481019763832 1104 98-99 0.880053623746111 1104 100-101 1.0557296321110599 1105 1 -0.040697005111471896 1105 2 0.01641737268219501 1105 3 0.23005285314770418 1105 4 0.17977517306371738 1105 5 0.26104674381669923 1105 6 0.22008483938894585 1105 7 0.25616548712182663 1105 8 0.32629566722329884 1105 9 0.26347025949902303 1105 10-11 0.16433366357658485 1105 12-13 0.15608913486824605 1105 14-15 0.08946722862574319 1105 16-17 0.18729934385850555 1105 18-19 0.05722540056352443 1105 20-21 0.34599825624601976 1105 22-23 0.3853778401851713 1105 24-25 0.3882800856981561 1105 26-27 0.5828136974717921 1105 28-29 0.5360077001386188 1105 30-31 0.5192545902081775 1105 32-33 0.5827279592780599 1105 34-35 0.5787466834848729 1105 36-37 0.5429478452839192 1105 38-39 0.636911407393363 1105 40-41 0.5787459097912162 1105 42-43 0.6758137945108658 1105 44-45 0.5198872446746705 1105 46-47 0.5901888197138945 1105 48-49 0.7152882572652643 1105 50-51 0.7265480820990717 1105 52-53 0.5423779965630899 1105 54-55 0.5889713033695649 1105 56-57 0.6291761802964508 1105 58-59 0.7073048727599094 1105 60-61 0.6624211390408057 1105 62-63 0.5225275594668553 1105 64-65 0.6204542000019053 1105 66-67 0.6357779668689076 1105 68-69 0.603037082825562 1105 70-71 0.4398370169865409 1105 72-73 0.5827703748136699 1105 74-75 0.5280245336415064 1105 76-77 0.37546743363515134 1105 78-79 0.5146474760991779 1105 80-81 0.505281791446901 1105 82-83 0.6577194023496631 1105 84-85 0.5883866263904203 1105 86-87 0.6725930113648388 1105 88-89 0.7279000636435136 1105 90-91 0.7169259238235739 1105 92-93 0.8158327643908017 1105 94-95 0.8922910759667957 1105 96-97 0.9827550485696754 1105 98-99 1.117853375916546 1105 100-101 1.3614094660870677 1106 1 0.09655039046742075 1106 2 0.19008644932263152 1106 3 0.21424833650241482 1106 4 0.4311702943309754 1106 5 0.26911518230237164 1106 6 0.2571773700377449 1106 7 0.2372553924358698 1106 8 0.19439324073128006 1106 9 0.16690723730747692 1106 10-11 0.17509526728164815 1106 12-13 0.2225874720630543 1106 14-15 0.23262936921118182 1106 16-17 0.22811771153884308 1106 18-19 0.13291201513401063 1106 20-21 0.20144887957305713 1106 22-23 0.3404562041766823 1106 24-25 0.32787034380231006 1106 26-27 0.20465165975210198 1106 28-29 0.23182479507926246 1106 30-31 0.33729973085505804 1106 32-33 0.36088917980639934 1106 34-35 0.3361284661820818 1106 36-37 0.4230580600253262 1106 38-39 0.5539767356057226 1106 40-41 0.34702171345053756 1106 42-43 0.31310708542002175 1106 44-45 0.3820057551986267 1106 46-47 0.4399496407073471 1106 48-49 0.387344061972577 1106 50-51 0.44743096273587213 1106 52-53 0.4227268275959446 1106 54-55 0.16264323659556723 1106 56-57 0.3080074511415489 1106 58-59 0.5650769826067723 1106 60-61 0.48151113768670584 1106 62-63 0.4494699355051992 1106 64-65 0.5276643617620671 1106 66-67 0.654903273199178 1106 68-69 0.5756441117751443 1106 70-71 0.46486068865453944 1106 72-73 0.5299175203678494 1106 74-75 0.5785300776038582 1106 76-77 0.47134634480078574 1106 78-79 0.4723469992823581 1106 80-81 0.5180509081512135 1106 82-83 0.4954071529594941 1106 84-85 0.603181015669275 1106 86-87 0.6548630055104532 1106 88-89 0.6153002345798768 1106 90-91 0.5561390803750044 1106 92-93 0.7040162383300306 1106 94-95 0.6580983882667226 1106 96-97 0.666054671564897 1106 98-99 0.7650012777311481 1106 100-101 0.9171599627996301 1107 1 0.02680701799881291 1107 2 0.06228072484568159 1107 3 0.11407605661295861 1107 4 0.3067529198578285 1107 5 0.1695028786819961 1107 6 0.19076297820051735 1107 7 0.16877113810168964 1107 8 0.13610847274857463 1107 9 0.09532032834877668 1107 10-11 0.1453584706811455 1107 12-13 0.10347039211880826 1107 14-15 0.290196882935831 1107 16-17 0.21015510112820124 1107 18-19 0.1944556724703972 1107 20-21 0.14802456253037377 1107 22-23 0.12502004110314147 1107 24-25 0.36474390530924694 1107 26-27 0.1323953286818309 1107 28-29 0.11135098645826957 1107 30-31 0.23101941035022122 1107 32-33 0.5134612693210983 1107 34-35 0.2375609214927792 1107 36-37 0.2980700399791658 1107 38-39 0.4377425506789976 1107 40-41 0.16454281105662005 1107 42-43 0.25882969747463136 1107 44-45 0.47842776160498346 1107 46-47 0.3621917361032345 1107 48-49 0.3949775938782878 1107 50-51 0.3025820553339642 1107 52-53 0.41946561501666224 1107 54-55 0.42009746808709636 1107 56-57 0.4959952298878534 1107 58-59 0.6220534589745519 1107 60-61 0.6336906371443334 1107 62-63 0.49960172464104247 1107 64-65 0.6043423697439323 1107 66-67 0.7044752175692715 1107 68-69 0.6125462971747133 1107 70-71 0.4954576661047341 1107 72-73 0.510393474421921 1107 74-75 0.5783211275710229 1107 76-77 0.5751946250895941 1107 78-79 0.404579639763778 1107 80-81 0.3892956156241496 1107 82-83 0.39415965796652586 1107 84-85 0.46345109476449764 1107 86-87 0.6654326649837934 1107 88-89 0.5822920558088427 1107 90-91 0.5479526383127578 1107 92-93 0.635985275992482 1107 94-95 0.5677742879520551 1107 96-97 0.7744739814865902 1107 98-99 0.7117051764403364 1107 100-101 0.7856963590829338 1108 1 0.07985423920214885 1108 2 0.0996004776214221 1108 3 0.09146011762948802 1108 4 0.3514298918784178 1108 5 0.0991416130475784 1108 6 0.24991104391551744 1108 7 0.27594986242991126 1108 8 0.19586103770060248 1108 9 0.20497273122557402 1108 10-11 0.2832934258401565 1108 12-13 0.06609645850246437 1108 14-15 0.270202549668511 1108 16-17 0.1554714633464087 1108 18-19 0.22187161269540923 1108 20-21 0.19449585493902788 1108 22-23 0.12633596871495456 1108 24-25 0.3614572753943648 1108 26-27 0.32138636341548477 1108 28-29 0.22401208023523367 1108 30-31 0.3182701247630604 1108 32-33 0.4148371659313028 1108 34-35 0.2945703106555513 1108 36-37 0.3993447580904217 1108 38-39 0.4012488297557937 1108 40-41 0.4596681947650936 1108 42-43 0.46324531148902537 1108 44-45 0.35304394227900104 1108 46-47 0.4381811580658024 1108 48-49 0.5763997595961499 1108 50-51 0.4629905563607224 1108 52-53 0.34130902764628246 1108 54-55 0.35362737423981017 1108 56-57 0.4423249204905275 1108 58-59 0.6302535509847722 1108 60-61 0.5532849053605382 1108 62-63 0.5517182274668002 1108 64-65 0.4707070062213603 1108 66-67 0.571381039978494 1108 68-69 0.6478556116201233 1108 70-71 0.5794279497640282 1108 72-73 0.5524987665717624 1108 74-75 0.6686083822112892 1108 76-77 0.6441286169654319 1108 78-79 0.5656946149524664 1108 80-81 0.5098819491867133 1108 82-83 0.5440745563572378 1108 84-85 0.49628312372152195 1108 86-87 0.5536702193701366 1108 88-89 0.5254086196200234 1108 90-91 0.4598223507351129 1108 92-93 0.5323693896038222 1108 94-95 0.6976338079640385 1108 96-97 0.7107542176188737 1108 98-99 0.724102407647603 1108 100-101 0.9654983778231951 1109 1 -0.17790591101628905 1109 2 0.13131417765504239 1109 3 0.09830906513207083 1109 4 0.18728309363069684 1109 5 0.12446610137067182 1109 6 0.10137762898715863 1109 7 0.10271132729877763 1109 8 0.1467689660774596 1109 9 0.1540116629170143 1109 10-11 0.09004801526128858 1109 12-13 0.1568333069837209 1109 14-15 0.05550633327680998 1109 16-17 0.02000111683633321 1109 18-19 0.20786951300061673 1109 20-21 0.22502101308695188 1109 22-23 0.10767575650324801 1109 24-25 0.2112650059426997 1109 26-27 0.22557898846169167 1109 28-29 0.029969587285023636 1109 30-31 0.1560232069255889 1109 32-33 0.28385556957614 1109 34-35 0.279393367501406 1109 36-37 0.2603856299439542 1109 38-39 0.33896940762814864 1109 40-41 0.3381401896026617 1109 42-43 0.242825709927601 1109 44-45 0.2349559191468913 1109 46-47 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46-47 -0.2941396350976291 2313 48-49 -0.30250800079867446 2313 50-51 -0.4195524178395118 2313 52-53 -0.48375699137776706 2313 54-55 -0.4902791054164375 2313 56-57 -0.40075427997623336 2313 58-59 -0.4124503219280129 2313 60-61 -0.4570587188314583 2313 62-63 -0.487277127992364 2313 64-65 -0.5101360246972959 2313 66-67 -0.5933270397200339 2313 68-69 -0.6710292513374618 2313 70-71 -0.5882932537778558 2313 72-73 -0.5127193384637074 2313 74-75 -0.5929778802750647 2313 76-77 -0.6936434468058614 2313 78-79 -0.591722521600694 2313 80-81 -0.7289216004242896 2313 82-83 -0.5903003822316819 2313 84-85 -0.5961104580841301 2313 86-87 -0.697556528278966 2313 88-89 -0.7584980192814932 2313 90-91 -0.6730307112804184 2313 92-93 -0.586937202269521 2313 94-95 -0.5966516584992476 2313 96-97 -0.5903144706269998 2313 98-99 -0.556251740285159 2313 100-101 -0.6371986780366612 2314 1 -0.4995069476905343 2314 2 -0.758648814800079 2314 3 -0.43795164707639245 2314 4 -0.7583801603342337 2314 5 -0.5856449383629823 2314 6 -0.4279951367954311 2314 7 -0.3207048494809044 2314 8 -0.2904190918603362 2314 9 -0.26524511099208326 2314 10-11 -0.45652587342723905 2314 12-13 -0.326923164667285 2314 14-15 -0.5166004727389932 2314 16-17 -0.5514371338600554 2314 18-19 -2.2275269455814737 2314 20-21 -0.9138996788867999 2314 22-23 -0.6181049420943907 2314 24-25 -0.8162426346895231 2314 26-27 -0.7305012363352006 2314 28-29 -0.42978789728574185 2314 30-31 -0.39699211353507735 2314 32-33 -0.5072966302656567 2314 34-35 -0.6789165697662369 2314 36-37 -0.6631763159984132 2314 38-39 -0.4408283346169881 2314 40-41 -0.34118294298026086 2314 42-43 -0.7228867885630805 2314 44-45 -0.64560795464444 2314 46-47 -0.5589399936778605 2314 48-49 -0.6150794115407834 2314 50-51 -0.4854781875724967 2314 52-53 -0.4751861087094582 2314 54-55 -0.4803113601909068 2314 56-57 -0.4359019695359372 2314 58-59 -0.5158824828972541 2314 60-61 -0.4394011085968934 2314 62-63 -0.5645047338386178 2314 64-65 -0.5954777662806556 2314 66-67 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2315 24-25 -1.7865131611486262 2315 26-27 -1.4349729462017962 2315 28-29 -1.195883347771705 2315 30-31 -1.3608341282629723 2315 32-33 -1.5177907195670457 2315 34-35 -1.6076214204719363 2315 36-37 -1.3680807124155336 2315 38-39 -1.4540577825269025 2315 40-41 -2.4092315780543885 2315 42-43 -1.9823010347205994 2315 44-45 -1.6918258010186378 2315 46-47 -1.3877991350039522 2315 48-49 -1.8363129172769561 2315 50-51 -1.5949012736646395 2315 52-53 -1.5745562668126851 2315 54-55 -1.5296697001352477 2315 56-57 -1.5803113017095356 2315 58-59 -1.675605038138574 2315 60-61 -1.3639323285175173 2315 62-63 -1.231149111192348 2315 64-65 -1.182172419298407 2315 66-67 -1.3035721308732349 2315 68-69 -1.5206820581155363 2315 70-71 -1.1685536391441005 2315 72-73 -1.0598556238278825 2315 74-75 -1.340100647763613 2315 76-77 -1.2876965278657124 2315 78-79 -1.410956236033357 2315 80-81 -1.4457799888261071 2315 82-83 -1.2867182927831102 2315 84-85 -1.3663941129495853 2315 86-87 -1.3833459765568996 2315 88-89 -1.3759346463171411 2315 90-91 -1.426609284518566 2315 92-93 -1.5227165620622358 2315 94-95 -1.4166163366461824 2315 96-97 -1.6060122230826614 2315 98-99 -1.6521191425141062 2315 100-101 -1.8572612196994136 2316 1 -0.8293985075109376 2316 2 -0.960496404207845 2316 3 -1.2944588683170544 2316 4 -1.257434618160353 2316 5 -0.803371557806571 2316 6 -0.7694182588136016 2316 7 -0.8991024526665754 2316 8 -0.9619593530461046 2316 9 -1.3079498478882243 2316 10-11 -1.307305135255973 2316 12-13 -1.0862823437205478 2316 14-15 -1.159925080068838 2316 16-17 -1.8713603153123017 2316 18-19 -1.3100852720258516 2316 20-21 -1.653733412049398 2316 22-23 -1.1834969503719464 2316 24-25 -1.1204711668298515 2316 26-27 -1.289280527880976 2316 28-29 -1.6300571066503906 2316 30-31 -1.5671775478631176 2316 32-33 -1.6278954691398653 2316 34-35 -2.021138337227697 2316 36-37 -1.7584606270223588 2316 38-39 -1.7278773785387713 2316 40-41 -2.141414870967772 2316 42-43 -2.199447135766171 2316 44-45 -2.453029042396153 2316 46-47 -2.324803489806534 2316 48-49 -2.9178953712120546 2316 50-51 -2.397795697932935 2316 52-53 -3.1207553656213705 2316 54-55 -4.314385593356203 2316 56-57 -3.012368978218717 2316 58-59 -2.5150818112921556 2316 60-61 -2.160767052329028 2316 62-63 -2.7805314035310182 2316 64-65 -2.6975068322470825 2316 66-67 -2.4710259046288883 2316 68-69 -2.4473803724357097 2316 70-71 -2.6274236908974196 2316 72-73 -5.730013326605036 2316 74-75 -3.520196937422355 2316 76-77 -2.9299248685705024 2316 78-79 -2.8269086988371654 2316 80-81 -2.727216957038987 2316 82-83 -2.77121802622149 2316 84-85 -2.784679978788784 2316 86-87 -2.5772761041131353 2316 88-89 -2.4037613778426596 2316 90-91 -2.450025505778285 2316 92-93 -2.8113646669101264 2316 94-95 -3.0827062197008956 2316 96-97 -2.9376218317523914 2316 98-99 -3.243972424084678 2316 100-101 -3.3379847708499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71817.0 3 12194.0 4 10175.0 5 9760.0 6 11246.0 7 13719.0 8 17355.0 9 20661.0 10 24384.0 11 29328.0 12 31172.0 13 35979.0 14 41569.0 15 51165.0 16 66853.0 17 91444.0 18 137136.0 19 207714.0 20 277914.0 21 198840.0 22 108159.0 23 92042.0 24 94514.0 25 99974.0 26 107076.0 27 119895.0 28 138841.0 29 162027.0 30 191820.0 31 229881.0 32 280049.0 33 347150.0 34 450157.0 35 630220.0 36 928657.0 37 985032.0 38 733700.0 39 118960.0 40 122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 9.664781107165103 51.81414045524221 23.019210842662012 15.50186759493067 2 15.486707959052252 53.787870232774004 16.199558579406983 14.525863228766758 3 48.88407313202243 19.18681748602155 17.252651141079227 14.676458240876796 4 15.44948360196527 17.450412585267298 18.167094730336636 48.9330090824308 5 15.22922816253618 51.217426531865776 19.142045316722722 14.411299988875328 6 49.04754501679786 18.58808749805669 17.142614135856647 15.221753349288802 7 16.116789224964997 17.912544729700354 51.78852568760301 14.182140357731631 8 48.526907303525476 18.677810162293596 18.178262933609574 14.617019600571348 9 48.33712904028096 18.335481197867118 18.584260790718997 14.743128971132924 10-11 14.925554885388545 34.96725053447426 35.02056029478178 15.086634285355407 12-13 14.691241377137645 18.681623137527247 35.00316853512195 31.623966950213156 14-15 14.882978532088528 51.83657269567392 18.142011392172304 15.138437380065245 16-17 31.57002721817839 18.45630441091727 18.220825210116367 31.75284316078798 18-19 15.1168346585211 34.94618058096399 18.22871563066463 31.70826912985028 20-21 31.82317783055809 35.077067513815344 18.228363582437616 14.871391073188947 22-23 31.761689997792498 18.41769033003249 34.88439051800451 14.936229154170505 24-25 14.96756207356889 34.8753734462358 18.811969567676883 31.34509491251843 26-27 15.237987529404123 24.799918165285774 44.614176219740706 15.347918085569399 28-29 28.058618977897925 18.363831445945568 38.45176646283492 15.125783113321589 30-31 25.17760757970707 19.986869120681185 38.82681034874378 16.008712950867967 32-33 19.193816948124624 22.068423523975298 40.84950003403736 17.88825949386272 34-35 18.31693576435891 21.31790586936175 41.412521943390495 18.952636422888848 36-37 18.338618229354847 21.39261336337147 41.55277341192678 18.7159949953469 38-39 18.892344709667384 21.49385255383545 41.065178706321326 18.54862403017584 40-41 18.56242881288551 21.27394491506674 41.24493524041341 18.918691031634342 42-43 19.050401734780138 21.67704471933894 41.200463784290555 18.072089761590373 44-45 18.399591027439726 21.727315343362946 41.05023049653915 18.822863132658174 46-47 18.714636182572047 21.566847975712918 40.797148464117804 18.92136737759723 48-49 18.862697984452222 21.73904682059852 40.73076149109732 18.66749370385194 50-51 19.006964528205962 21.72165885490413 40.039626484773756 19.231750132116154 52-53 19.320573531905843 22.611042059740374 38.77825340306198 19.2901310052918 54-55 20.09143947669076 23.606318999783433 36.913304121235875 19.388937402289937 56-57 20.545473764437762 24.51664273045982 35.36068859349102 19.577194911611397 58-59 20.482424742299077 25.788767993664784 33.8846636844673 19.844143579568843 60-61 20.479029514862653 26.175172020965782 33.31815608883106 20.027642375340506 62-63 20.48668032358478 25.982539902442888 33.75676701779014 19.7740127561822 64-65 20.33982127770359 25.676842615503443 33.83822145141415 20.145114655378826 66-67 20.320610762555006 25.17103033640118 34.65648543258922 19.8518734684546 68-69 19.83218316782596 24.74522725503781 35.236134482298006 20.186455094838223 70-71 19.722230797625645 24.39506761997897 35.78452200155444 20.098179580840938 72-73 19.59544966226155 24.39528505387329 35.37593409198309 20.63333119188207 74-75 20.134308441139886 24.64866503374435 34.88445390594785 20.33257261916791 76-77 20.372031533751027 25.123842226999223 34.26292438727286 20.241201851976896 78-79 20.28187612209197 25.191971143444157 34.14656206525197 20.379590669211904 80-81 20.29268110491737 25.553217514597893 33.800797516780314 20.35330386370443 82-83 21.175596626527483 25.714146750778877 32.945241240996275 20.165015381697366 84-85 20.664186483396037 26.509366187516072 32.29934450972017 20.527102819367727 86-87 20.595229376363687 26.21456301431176 32.36212704525257 20.828080564071985 88-89 20.635803906599815 26.054708507305836 32.3711627975684 20.938324788525946 90-91 20.466528158200674 26.230751499478643 32.12146090568817 21.181259436632516 92-93 20.7396696486635 26.356114791351388 32.18423017023434 20.719985389750764 94-95 20.67389882549655 26.206246691016972 31.84350647748888 21.276348005997594 96-97 20.532233349297275 26.028395941303035 32.4468211848275 20.992549524572187 98-99 20.97958939666171 26.096067382575644 31.775636858942224 21.148706361820423 100-101 21.285960292933908 26.062424645484565 31.620632224819907 21.03098283676162 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11156.0 1 6737.0 2 1734.5 3 1069.5 4 934.5 5 812.0 6 886.5 7 1041.0 8 945.0 9 993.0 10 1404.0 11 9282.0 12 174294.0 13 195554.0 14 53195.5 15 48443.5 16 51878.0 17 55391.5 18 57600.0 19 58860.5 20 59887.0 21 60379.0 22 61000.0 23 62445.5 24 64845.5 25 68460.5 26 72896.0 27 78005.0 28 84377.5 29 90731.0 30 95888.5 31 101831.5 32 108606.5 33 116636.5 34 129371.0 35 137021.0 36 140984.0 37 148761.0 38 156143.5 39 164237.5 40 173319.0 41 181444.5 42 187942.5 43 194118.0 44 202721.0 45 209605.5 46 213263.5 47 218726.0 48 222650.5 49 222106.0 50 219686.5 51 218932.0 52 216796.5 53 218319.5 54 211999.5 55 197321.0 56 197910.5 57 186628.0 58 161476.0 59 136140.0 60 109441.5 61 88727.0 62 69470.5 63 53362.5 64 39360.0 65 28033.0 66 19224.5 67 12485.0 68 7887.0 69 4927.0 70 3345.5 71 2468.0 72 1976.0 73 1703.0 74 1598.5 75 1518.5 76 1407.5 77 1284.5 78 1214.5 79 1155.0 80 1070.0 81 973.5 82 896.0 83 850.5 84 766.0 85 650.5 86 566.0 87 482.5 88 389.5 89 304.5 90 241.5 91 190.0 92 144.0 93 113.0 94 80.5 95 59.0 96 41.0 97 22.5 98 12.0 99 7.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09999023500212643 2 0.08288407610234776 3 0.08931978083500065 4 0.19333581381924111 5 0.07598867817450539 6 0.09271872446003811 7 0.09767783892935505 8 0.08645018088927231 9 0.0791926004440079 10-11 0.07704040048471164 12-13 0.07019375789575301 14-15 0.07703343543629969 16-17 0.06712913659448973 18-19 0.059147191114381284 20-21 0.054160216451416485 22-23 0.044088756447719445 24-25 0.046025039906244875 26-27 0.045439975839640066 28-29 0.044228057415958685 30-31 0.03464415080109897 32-33 0.03848189247609003 34-35 0.051604043684226435 36-37 0.04706283211962721 38-39 0.05138812718345562 40-41 0.04887374470673733 42-43 0.04711855250692291 44-45 0.05081002816526277 46-47 0.04456934478814482 48-49 0.04018832933702072 50-51 0.044534519546085005 52-53 0.0401743992401968 54-55 0.04367085354300172 56-57 0.042215158424901665 58-59 0.04975134080664454 60-61 0.04855335247978708 62-63 0.05331048054515713 64-65 0.0494100534344584 66-67 0.04383104965647685 68-69 0.04373353897870938 70-71 0.052760241720612125 72-73 0.040146539046548954 74-75 0.04772451171876361 76-77 0.046547418537142025 78-79 0.04469471565956013 80-81 0.04426984770643045 82-83 0.043566377816822294 84-85 0.04396338557630412 86-87 0.048999115578152654 88-89 0.04176243027812413 90-91 0.044673820514324246 92-93 0.04062712738697433 94-95 0.04399124576995197 96-97 0.029420364492127475 98-99 0.036253076984262195 100-101 0.0249627335084718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7178701.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.806266429599134 #Duplication Level Percentage of deduplicated Percentage of total 1 93.02177706773598 49.12132743593642 2 4.089910332274234 4.319457893584871 3 0.9877058344116733 1.5647117233803705 4 0.47033589806188675 0.993467309778431 5 0.29216226435081744 0.771399918599212 6 0.2147095601720399 0.6802806143656073 7 0.15938946328249814 0.5891733724916484 8 0.12132714465369475 0.5125466820580415 9 0.09913352363335651 0.47113841349791874 >10 0.519972352524251 4.347875371762546 >50 0.008688364366345201 0.31830900703157666 >100 0.00813847057223599 1.0165551354178215 >500 0.003032484678215731 1.084780022921187 >1k 0.003228128850848518 3.317722215693572 >5k 3.9128834555739606E-4 1.4896095961815985 >10k+ 9.782208638934902E-5 29.4016452872991 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1113788 15.515174681324659 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 24571 0.34227640906063644 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9869 0.1374761255553059 No Hit TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT 8813 0.12276594330924216 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 8508 0.11851726377794533 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA 7493 0.10437821550166249 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC 7255 0.10106285245756857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.3930096823923994E-5 0.0 0.0 1.3930096823923994E-5 0.0 2 2.786019364784799E-5 0.0 0.0 1.3930096823923994E-5 0.0 3 4.179029047177198E-5 0.0 0.0 1.3930096823923994E-5 0.0 4 4.179029047177198E-5 0.0 0.0 1.3930096823923994E-5 0.0 5 4.179029047177198E-5 0.0 0.0 1.3930096823923994E-5 0.0 6 5.572038729569598E-5 0.0 0.0 1.3930096823923994E-5 0.0 7 5.572038729569598E-5 0.0 0.0 2.786019364784799E-5 0.0 8 5.572038729569598E-5 0.0 0.0 2.786019364784799E-5 0.0 9 8.358058094354396E-5 0.0 0.0 4.179029047177198E-5 0.0 10-11 8.358058094354396E-5 0.0 0.0 4.179029047177198E-5 0.0 12-13 1.1144077459139195E-4 0.0 0.0 4.179029047177198E-5 0.0 14-15 1.1144077459139195E-4 0.0 0.0 4.179029047177198E-5 0.0 16-17 1.1840582300335395E-4 1.3930096823923994E-5 0.0 4.179029047177198E-5 0.0 18-19 1.3233591982727796E-4 2.786019364784799E-5 0.0 4.179029047177198E-5 0.0 20-21 1.4626601665120194E-4 2.786019364784799E-5 0.0 4.179029047177198E-5 0.0 22-23 1.6019611347512592E-4 2.786019364784799E-5 0.0 4.179029047177198E-5 0.0 24-25 1.741262102990499E-4 2.786019364784799E-5 0.0 4.179029047177198E-5 0.0 26-27 2.1591650077082192E-4 2.786019364784799E-5 0.0 4.179029047177198E-5 0.0 28-29 2.228815491827839E-4 2.786019364784799E-5 0.0 4.179029047177198E-5 0.0 30-31 2.4377669441866988E-4 2.786019364784799E-5 0.0 4.179029047177198E-5 0.0 32-33 2.577067912425939E-4 2.786019364784799E-5 0.0 4.179029047177198E-5 6.965048411961997E-6 34-35 2.786019364784799E-4 3.482524205980999E-5 0.0 5.572038729569598E-5 1.3930096823923994E-5 36-37 2.994970817143659E-4 4.179029047177198E-5 0.0 5.572038729569598E-5 2.786019364784799E-5 38-39 3.2039222695025185E-4 4.179029047177198E-5 0.0 6.268543570765798E-5 3.482524205980999E-5 40-41 3.2735727536221384E-4 4.179029047177198E-5 0.0 6.965048411961997E-5 4.875533888373398E-5 42-43 3.7611261424594784E-4 4.179029047177198E-5 0.0 6.965048411961997E-5 5.572038729569598E-5 44-45 4.1790290471771985E-4 4.179029047177198E-5 0.0 7.661553253158197E-5 6.965048411961997E-5 46-47 4.527281467775298E-4 4.179029047177198E-5 0.0 8.358058094354396E-5 6.965048411961997E-5 48-49 5.363087277210738E-4 4.179029047177198E-5 0.0 8.358058094354396E-5 6.965048411961997E-5 50-51 5.989941634287317E-4 4.875533888373398E-5 0.0 8.358058094354396E-5 6.965048411961997E-5 52-53 6.825747443722757E-4 5.572038729569598E-5 0.0 8.358058094354396E-5 6.965048411961997E-5 54-55 8.149106641995537E-4 5.572038729569598E-5 0.0 8.358058094354396E-5 6.965048411961997E-5 56-57 9.681417292627175E-4 5.572038729569598E-5 0.0 8.358058094354396E-5 6.965048411961997E-5 58-59 0.0011004776490899956 5.572038729569598E-5 0.0 8.358058094354396E-5 6.965048411961997E-5 60-61 0.0013651494887445513 5.572038729569598E-5 0.0 1.1144077459139195E-4 6.965048411961997E-5 62-63 0.0015949960863392973 6.268543570765798E-5 0.0 1.1144077459139195E-4 6.965048411961997E-5 64-65 0.0018666329744058152 6.965048411961997E-5 0.0 1.1144077459139195E-4 6.965048411961997E-5 66-67 0.002124339765648409 7.661553253158197E-5 0.0 1.1144077459139195E-4 6.965048411961997E-5 68-69 0.002298465975947459 8.358058094354396E-5 0.0 1.1144077459139195E-4 8.358058094354396E-5 70-71 0.002507417428306319 8.358058094354396E-5 0.0 1.1144077459139195E-4 8.358058094354396E-5 72-73 0.002772089267960875 1.0447572617942996E-4 0.0 1.1840582300335395E-4 1.1840582300335395E-4 74-75 0.0031621319790307465 1.1144077459139195E-4 0.0 1.2537087141531594E-4 1.2537087141531594E-4 76-77 0.0035382445932766945 1.2537087141531594E-4 0.0 1.2537087141531594E-4 1.2537087141531594E-4 78-79 0.004130273708293464 1.2537087141531594E-4 0.0 1.3233591982727796E-4 1.2537087141531594E-4 80-81 0.005244681454207384 1.3233591982727796E-4 0.0 1.3930096823923995E-4 1.2537087141531594E-4 82-83 0.0065123202651844675 1.3930096823923995E-4 0.0 1.3930096823923995E-4 1.3930096823923995E-4 84-85 0.008281442561822814 1.3930096823923995E-4 0.0 1.3930096823923995E-4 1.3930096823923995E-4 86-87 0.010733139602833439 1.3930096823923995E-4 0.0 1.3930096823923995E-4 1.4626601665120194E-4 88-89 0.014724112342887662 1.3930096823923995E-4 0.0 1.3930096823923995E-4 1.5323106506316393E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 241175 0.0 91.68717 5 AAGCAGT 242315 0.0 91.67724 1 TGGTATC 241890 0.0 91.48013 7 GGTATCA 242035 0.0 91.452484 8 GTGGTAT 242145 0.0 91.439285 6 GTATCAA 242145 0.0 91.35158 9 AGCAGTG 243525 0.0 91.15797 2 GCAGTGG 243200 0.0 91.067726 3 CAGTGGT 243550 0.0 90.64308 4 TCAACGC 241435 0.0 46.46837 12-13 AACGCAG 242360 0.0 46.32796 14-15 CGCAGAG 243380 0.0 46.219593 16-17 CAACGCA 241675 0.0 46.15291 12-13 ATCAACG 241660 0.0 46.13933 10-11 TATCAAC 242775 0.0 46.12802 10-11 ACGCAGA 242400 0.0 46.046925 14-15 CAGAGTA 241375 0.0 45.977608 18-19 GAGTACT 141425 0.0 45.777058 20-21 AGAGTAC 237110 0.0 45.706875 18-19 GTACTTT 140660 0.0 45.576385 22-23 >>END_MODULE