FastQCFastQC Report
Sat 18 Jun 2016
SRR578550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7178701
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281990.3928148003378327No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80520.11216513962623599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22050.025.6705021
TATGCCG10750.023.64167448-49
ATCGGAA25200.022.430242
CTCGTAT11450.021.36596744-45
TCTCGTA12200.020.24674642-43
ACACGTC13850.019.89058712-13
CGTCACG2651.8590072E-819.7168433
CAACGCA51700.019.5685412-13
TCGGAAG29250.019.1622583
ATCAACG53050.019.02579910-11
CTCGCGT1708.931238E-1018.1624652-53
CGCGTAG1608.294592E-917.81243754-55
TCAACGC49450.017.33725410-11
ATGCCGT15100.017.1455948-49
ACGCAGA61500.016.75922814-15
CGGAAGA34800.016.3791944
CGTACGC1601.6073864E-716.3274420-21
TCGTATG16300.016.17432644-45
CGTATGC16600.015.73937546-47
GGTATCA68650.015.7057158
Produced by FastQC (version 0.11.5)