##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578550_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7178701 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.499595957541622 31.0 31.0 34.0 30.0 34.0 2 31.81080017122875 31.0 31.0 34.0 30.0 34.0 3 31.882644227695234 31.0 31.0 34.0 30.0 34.0 4 35.555872991506405 37.0 35.0 37.0 33.0 37.0 5 35.380412835135495 37.0 35.0 37.0 33.0 37.0 6 35.37569624365188 37.0 35.0 37.0 32.0 37.0 7 35.355399813977485 37.0 35.0 37.0 32.0 37.0 8 35.34238074548585 37.0 35.0 37.0 32.0 37.0 9 36.91270426223352 39.0 37.0 39.0 33.0 39.0 10-11 36.87830284336957 39.0 37.0 39.0 32.5 39.0 12-13 36.85701389429647 39.0 37.0 39.0 32.0 39.0 14-15 38.042739626570324 40.0 37.0 41.0 33.0 41.0 16-17 37.98764574537928 40.0 37.0 41.0 33.0 41.0 18-19 37.895009417441955 40.0 37.0 41.0 33.0 41.0 20-21 37.834852015705906 40.0 37.0 41.0 32.0 41.0 22-23 37.74020432108817 40.0 37.0 41.0 32.0 41.0 24-25 37.64809357570402 39.0 36.0 41.0 32.0 41.0 26-27 37.466165884329214 39.0 36.0 41.0 32.0 41.0 28-29 37.297134537293026 39.0 36.0 41.0 31.5 41.0 30-31 37.16420617044783 39.0 36.0 41.0 31.0 41.0 32-33 37.034537933812814 39.0 36.0 40.5 31.0 41.0 34-35 36.88203046762918 39.0 35.5 40.0 30.0 41.0 36-37 36.731739837054086 39.0 35.0 40.0 30.0 41.0 38-39 36.56568542135966 38.0 35.0 40.0 30.0 41.0 40-41 36.39817113151808 38.0 35.0 40.0 30.0 41.0 42-43 36.332421422761584 38.0 35.0 40.0 30.0 41.0 44-45 36.35939426924175 38.0 35.0 40.0 30.0 41.0 46-47 36.23303261968983 38.0 35.0 40.0 30.0 41.0 48-49 36.08767212898267 38.0 35.0 40.0 29.0 41.0 50-51 35.8896123407285 38.0 34.5 40.0 28.5 41.0 52-53 35.952430460608404 38.0 35.0 40.0 29.0 41.0 54-55 35.93879108490519 38.0 35.0 40.0 28.5 41.0 56-57 35.77542907832489 38.0 34.0 40.0 28.0 41.0 58-59 35.56188696255771 38.0 34.0 40.0 28.0 41.0 60-61 35.29918637090471 38.0 34.0 40.0 28.0 41.0 62-63 35.01598318414432 37.0 34.0 40.0 27.5 41.0 64-65 34.689582976084395 37.0 33.0 40.0 27.0 41.0 66-67 34.34575962977146 36.0 33.0 39.0 26.0 41.0 68-69 33.98026460497519 36.0 33.0 39.0 26.0 40.5 70-71 33.59157290434578 35.0 32.0 39.0 26.0 40.0 72-73 33.19457099550462 35.0 32.0 38.0 26.0 40.0 74-75 32.78703173457148 35.0 32.0 37.0 25.0 39.5 76-77 31.431156277437935 34.0 30.0 36.0 23.0 39.0 78-79 32.07669542163687 34.5 31.0 36.0 25.0 39.0 80-81 31.958552180958648 35.0 31.0 36.0 24.5 38.5 82-83 31.651151440908322 34.5 31.0 35.5 24.0 37.0 84-85 31.33067828009552 34.0 31.0 35.0 23.5 37.0 86-87 30.988732083980096 34.0 31.0 35.0 22.0 36.5 88-89 30.66840943786348 34.0 31.0 35.0 20.0 36.0 90-91 30.34742448529337 34.0 30.0 35.0 19.5 36.0 92-93 29.989530417829073 34.0 30.0 35.0 18.0 35.5 94-95 29.618739309521317 34.0 30.0 35.0 12.5 35.0 96-97 29.22830592888602 34.0 29.5 35.0 3.5 35.0 98-99 28.750144211327367 34.0 29.0 35.0 2.0 35.0 100-101 27.644946766831495 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35791394517307396 1101 2 0.390548527910628 1101 3 0.35637980637270417 1101 4 0.28704904083217997 1101 5 0.31957124635183476 1101 6 0.28972397572533026 1101 7 0.324137003843191 1101 8 0.28988173762590463 1101 9 0.36237700533597206 1101 10-11 0.36514007118422853 1101 12-13 0.35118934477731756 1101 14-15 0.4631650455861305 1101 16-17 0.42397034157370683 1101 18-19 0.35865380348207054 1101 20-21 0.456745400158475 1101 22-23 0.4609868011946716 1101 24-25 0.4472292620983964 1101 26-27 0.3782017944968814 1101 28-29 0.4725392678895659 1101 30-31 0.5126472369993564 1101 32-33 0.44881840202433665 1101 34-35 0.4973949804222002 1101 36-37 0.5137888852917527 1101 38-39 0.4798722152509711 1101 40-41 0.4827388066496141 1101 42-43 0.4888798194141586 1101 44-45 0.5267105541018822 1101 46-47 0.38093329293144507 1101 48-49 0.32524586694877655 1101 50-51 0.40568091659518046 1101 52-53 0.3968514410156203 1101 54-55 0.4795832033085361 1101 56-57 0.6183482339112132 1101 58-59 0.5895076264141821 1101 60-61 0.5527117426233872 1101 62-63 0.4154460568665215 1101 64-65 0.45337206093101656 1101 66-67 0.46103486663312765 1101 68-69 0.5745784697923781 1101 70-71 0.6354193281998093 1101 72-73 0.5116553150562666 1101 74-75 0.3292711732289888 1101 76-77 0.2794799989114516 1101 78-79 0.4834218818211724 1101 80-81 0.4842814257351087 1101 82-83 0.47006830312145453 1101 84-85 0.5811580302046515 1101 86-87 0.5248477147847268 1101 88-89 0.4699721556290122 1101 90-91 0.5598087839368091 1101 92-93 0.5700526828789947 1101 94-95 0.6849969136817684 1101 96-97 0.6443383903811508 1101 98-99 0.658985074655984 1101 100-101 0.7909095827157664 1102 1 0.2845263565905469 1102 2 0.2509668479226157 1102 3 0.3175457462430309 1102 4 0.29145193132269753 1102 5 0.3325746853100995 1102 6 0.22131931846362818 1102 7 0.2059856068058039 1102 8 0.18113342775393448 1102 9 0.42964665323385276 1102 10-11 0.31377436666318914 1102 12-13 0.19781366028109204 1102 14-15 0.4172186729769649 1102 16-17 0.38177546767234816 1102 18-19 0.42453580484588826 1102 20-21 0.42538239653935506 1102 22-23 0.4121358330286782 1102 24-25 0.3682402671852216 1102 26-27 0.4782859368246548 1102 28-29 0.37750944536839626 1102 30-31 0.31943193563184735 1102 32-33 0.40453610928140193 1102 34-35 0.400258334302805 1102 36-37 0.35770175200412524 1102 38-39 0.39117697607478163 1102 40-41 0.3683368815175925 1102 42-43 0.3379662494764162 1102 44-45 0.32376386110780686 1102 46-47 0.3987067738880512 1102 48-49 0.410161194649703 1102 50-51 0.422465843430615 1102 52-53 0.43388551291575794 1102 54-55 0.3371031583045152 1102 56-57 0.4328615430970757 1102 58-59 0.5234316900840028 1102 60-61 0.47397928835181347 1102 62-63 0.40198463562461484 1102 64-65 0.39530799956124696 1102 66-67 0.4662399955542753 1102 68-69 0.4177167226406411 1102 70-71 0.36627054919063795 1102 72-73 0.44297568826416267 1102 74-75 0.25860967145546 1102 76-77 0.19803564544791996 1102 78-79 0.3587605454947749 1102 80-81 0.3718724479311497 1102 82-83 0.3410314381374242 1102 84-85 0.20090129288815817 1102 86-87 0.15378042464360675 1102 88-89 0.29905877485949617 1102 90-91 0.4201971147794268 1102 92-93 0.356955387954617 1102 94-95 0.2374637724592823 1102 96-97 0.2756781157989643 1102 98-99 0.36334775188484514 1102 100-101 0.5165894548260326 1103 1 0.22563150564046808 1103 2 0.2600378228756419 1103 3 0.2588351696420723 1103 4 0.1214602837558445 1103 5 0.19766034084199902 1103 6 0.17966412134746435 1103 7 0.18243056771125765 1103 8 0.15578832151673083 1103 9 0.35063103451076216 1103 10-11 0.3053240847778582 1103 12-13 0.27038015042626995 1103 14-15 0.3716594358969161 1103 16-17 0.3985439412269258 1103 18-19 0.39586456610990695 1103 20-21 0.34404569370283866 1103 22-23 0.34732311193567966 1103 24-25 0.29275780866932877 1103 26-27 0.2451178411727284 1103 28-29 0.3260343534429708 1103 30-31 0.37856224205886235 1103 32-33 0.33583730944774004 1103 34-35 0.3530896638917369 1103 36-37 0.3942665861505148 1103 38-39 0.4330507726078068 1103 40-41 0.393339396460334 1103 42-43 0.3277751220012064 1103 44-45 0.23506637223911753 1103 46-47 0.29376722204118977 1103 48-49 0.2690871629082281 1103 50-51 0.3520869262587141 1103 52-53 0.37268881140617793 1103 54-55 0.3731145378817331 1103 56-57 0.3499226601432568 1103 58-59 0.3245913833011116 1103 60-61 0.3094669376054924 1103 62-63 0.22632954690731566 1103 64-65 0.27347641877522477 1103 66-67 0.2693275855489219 1103 68-69 0.21169982059093684 1103 70-71 0.1462486108659533 1103 72-73 0.18811271029409937 1103 74-75 0.17586807405745475 1103 76-77 0.14372489057949522 1103 78-79 0.27278951273149943 1103 80-81 0.22654588763206718 1103 82-83 0.19788152919915092 1103 84-85 0.3002106606374966 1103 86-87 0.31300034401535726 1103 88-89 0.3165817590957438 1103 90-91 0.3217225259911416 1103 92-93 0.2899331113571293 1103 94-95 0.2685626088914894 1103 96-97 0.30382188523079634 1103 98-99 0.40529629088142727 1103 100-101 0.5355708736712828 1104 1 0.1418440150475213 1104 2 0.16012092969069514 1104 3 0.2028578478201375 1104 4 0.18406078467094034 1104 5 0.15007400832136852 1104 6 0.16705792945786158 1104 7 0.18669273040621448 1104 8 0.14646512763031438 1104 9 0.24711598032514104 1104 10-11 0.24404590174365381 1104 12-13 0.22537457370859926 1104 14-15 0.23850596687893955 1104 16-17 0.21537552588397801 1104 18-19 0.2794867888350794 1104 20-21 0.2787529682333698 1104 22-23 0.2991585523509599 1104 24-25 0.2747503298213303 1104 26-27 0.26119987364550923 1104 28-29 0.2571981079816723 1104 30-31 0.1866329240715885 1104 32-33 0.17033223573348977 1104 34-35 0.2504780860769813 1104 36-37 0.2309912428087202 1104 38-39 0.3185450580712441 1104 40-41 0.31318688599708366 1104 42-43 0.22708776052395763 1104 44-45 0.28145899415581255 1104 46-47 0.21835986908345717 1104 48-49 0.17369049433144568 1104 50-51 0.20513836556138187 1104 52-53 0.23833149080663674 1104 54-55 0.2191631811173309 1104 56-57 0.3501289784697974 1104 58-59 0.2780781630197211 1104 60-61 0.27822387023473993 1104 62-63 0.24184215391922947 1104 64-65 0.288706281117058 1104 66-67 0.2847147287136167 1104 68-69 0.3423214893071176 1104 70-71 0.20103648621469716 1104 72-73 0.2355945716112231 1104 74-75 0.24705585095944116 1104 76-77 0.297303053900297 1104 78-79 0.3316020441589629 1104 80-81 0.44118306859992273 1104 82-83 0.2954950264194274 1104 84-85 0.33495200994623175 1104 86-87 0.40792284671898216 1104 88-89 0.34147843319402327 1104 90-91 0.5181441057291281 1104 92-93 0.5220597339545527 1104 94-95 0.5023584473404163 1104 96-97 0.5653538168459491 1104 98-99 0.4521788778224085 1104 100-101 0.6665806876228828 1105 1 0.12718550726272326 1105 2 0.11343858954015928 1105 3 0.07934042770039795 1105 4 0.11205766109718951 1105 5 0.07218890760700702 1105 6 0.16615582356252645 1105 7 0.14914503241261912 1105 8 0.11242931106013287 1105 9 0.10291183601415099 1105 10-11 0.12816942978768964 1105 12-13 0.11274593609598327 1105 14-15 0.1469918086577522 1105 16-17 0.07930457709488081 1105 18-19 0.1993493645670199 1105 20-21 0.20210974263010684 1105 22-23 0.21908176996275586 1105 24-25 0.1899763685960565 1105 26-27 0.18234330855416658 1105 28-29 0.21213308070637282 1105 30-31 0.20844546244513396 1105 32-33 0.23913570691605202 1105 34-35 0.1717454428988603 1105 36-37 0.26565231958462476 1105 38-39 0.3300851258118982 1105 40-41 0.2884428777962711 1105 42-43 0.22321828452327708 1105 44-45 0.1695245295965151 1105 46-47 0.1509698064658096 1105 48-49 0.23405925400215466 1105 50-51 0.18924079907259994 1105 52-53 0.10812980967539687 1105 54-55 0.17258134035499495 1105 56-57 0.13762870841556918 1105 58-59 0.12373377660752993 1105 60-61 0.0054165162606381045 1105 62-63 0.024251459727643976 1105 64-65 0.16305423830567634 1105 66-67 0.10780478935620152 1105 68-69 0.09283664225478105 1105 70-71 0.15177941519681326 1105 72-73 0.22791531674555898 1105 74-75 0.2799809157778057 1105 76-77 0.19785956881680988 1105 78-79 0.2417429707540535 1105 80-81 0.15454817171857016 1105 82-83 0.16431968839175326 1105 84-85 0.3255760480451606 1105 86-87 0.28555106258354 1105 88-89 0.31537657808013364 1105 90-91 0.3158851832265981 1105 92-93 0.318039309227526 1105 94-95 0.2723027987008244 1105 96-97 0.3916083069653844 1105 98-99 0.5372438380743496 1105 100-101 0.583063806542075 1106 1 -0.0829680809850899 1106 2 -0.007723935411956262 1106 3 -0.060531048170844315 1106 4 0.028396549205126576 1106 5 -0.002201849356630703 1106 6 0.080207149344389 1106 7 0.0981192816377714 1106 8 0.11519274398352763 1106 9 -0.042125543680406 1106 10-11 -0.004490923765132493 1106 12-13 -0.00783036158417616 1106 14-15 0.2168449672564563 1106 16-17 0.13442617696057368 1106 18-19 -0.0015283312056837417 1106 20-21 0.07497392521187152 1106 22-23 0.07638299121222758 1106 24-25 0.15778666350106363 1106 26-27 0.28489496347059884 1106 28-29 0.19332253641464092 1106 30-31 0.11668698779444497 1106 32-33 0.15007994930476087 1106 34-35 0.13014126329482423 1106 36-37 -0.014629422905336753 1106 38-39 0.006371616183628248 1106 40-41 -0.03509812560432124 1106 42-43 -0.030550275845769193 1106 44-45 -0.021045390406897013 1106 46-47 0.03364360066591132 1106 48-49 0.09804692846265084 1106 50-51 0.058051837980166 1106 52-53 -0.08729926273857558 1106 54-55 -0.08373540545139946 1106 56-57 -0.025612218018501665 1106 58-59 -0.0016516025918420496 1106 60-61 0.15942495454989114 1106 62-63 0.03787201455612177 1106 64-65 0.040058516589148496 1106 66-67 -0.01889913526262177 1106 68-69 0.03028195226098518 1106 70-71 0.0340214599644213 1106 72-73 -0.03765997414051725 1106 74-75 -0.047917706760145506 1106 76-77 -0.15816851848015645 1106 78-79 -0.023006634288044836 1106 80-81 0.14172398420211252 1106 82-83 0.04978037392497825 1106 84-85 0.22895885108462366 1106 86-87 0.4233248835144039 1106 88-89 0.3292755693577618 1106 90-91 0.45775648404685043 1106 92-93 0.3716028871808774 1106 94-95 0.49846815837505076 1106 96-97 0.44598368209384986 1106 98-99 0.48805409763940943 1106 100-101 0.5053489086920706 1107 1 0.08385692738641382 1107 2 0.08678102413989919 1107 3 0.045145382717823423 1107 4 0.10275158583722543 1107 5 -0.005729838368701223 1107 6 -0.04445236660340868 1107 7 0.05955726114057569 1107 8 0.013999454665352573 1107 9 -0.0424099064925727 1107 10-11 -2.1021842893276244E-4 1107 12-13 -0.06036678564097997 1107 14-15 0.019236088894693637 1107 16-17 0.0904194537385905 1107 18-19 -0.023377356118970738 1107 20-21 -0.0665471149535577 1107 22-23 -0.12657540776026366 1107 24-25 -0.054571742912763455 1107 26-27 -0.030476729654481005 1107 28-29 -0.009590616643642136 1107 30-31 -0.004338486084833448 1107 32-33 -0.05370535140672672 1107 34-35 0.017811237054125684 1107 36-37 0.04294279863872674 1107 38-39 0.01648772065366444 1107 40-41 -0.032379110043194714 1107 42-43 0.0597798736223254 1107 44-45 0.0025226110373210986 1107 46-47 -0.012304515061366317 1107 48-49 -0.07196536131304754 1107 50-51 0.0252528634817466 1107 52-53 -0.07118652727879038 1107 54-55 -0.035405045437698845 1107 56-57 0.07788372976759916 1107 58-59 0.04979567577480282 1107 60-61 0.17199664709985996 1107 62-63 0.16524626782319984 1107 64-65 0.1270580284838374 1107 66-67 0.07379833724677809 1107 68-69 0.13595414874225042 1107 70-71 0.09070871666352076 1107 72-73 0.01953635827003808 1107 74-75 0.055811098365133205 1107 76-77 -0.007330239072604883 1107 78-79 0.08529891648176147 1107 80-81 0.19931005743081798 1107 82-83 0.24908759156092586 1107 84-85 0.14343929525870536 1107 86-87 0.1948046908967349 1107 88-89 0.14568767303695296 1107 90-91 0.15565745761688987 1107 92-93 0.1819459716677052 1107 94-95 0.07359640831779402 1107 96-97 0.14740944476147178 1107 98-99 0.14348891832689858 1107 100-101 0.27880614049355756 1108 1 0.03428802405778697 1108 2 0.027734464362350053 1108 3 0.04313421106166615 1108 4 0.04770122545362909 1108 5 -0.0502531018855521 1108 6 -0.05827835527498593 1108 7 0.03866378514137381 1108 8 3.969372001932925E-5 1108 9 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-0.8979416854751641 2316 96-97 -1.0246087129924035 2316 98-99 -1.2365597243885027 2316 100-101 -1.5877028700079094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111.0 3 62.0 4 223.0 5 630.0 6 1126.0 7 1937.0 8 3274.0 9 4949.0 10 6909.0 11 8781.0 12 10805.0 13 12730.0 14 14760.0 15 16776.0 16 19260.0 17 22520.0 18 26257.0 19 29320.0 20 32276.0 21 34039.0 22 38429.0 23 45838.0 24 56686.0 25 71711.0 26 90808.0 27 115732.0 28 144745.0 29 178628.0 30 218633.0 31 265659.0 32 324252.0 33 402585.0 34 519920.0 35 737060.0 36 925969.0 37 1302061.0 38 1220178.0 39 272648.0 40 414.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.75130522269035 31.48633075239279 27.785252800622516 21.977111224294347 2 22.7654418257565 29.244218417788957 28.15743405387688 19.832905702577666 3 21.06386656861736 28.485724645726297 28.615121315123726 21.83528747053262 4 21.662995277134538 28.950344787427866 28.152878025309253 21.23378191012834 5 22.30719457281462 28.80989876265467 27.930655726857673 20.95225093767304 6 21.98044415766703 28.67743976490196 27.85527640262992 21.486839674801097 7 22.085499869683943 29.02970885679735 27.7570830711573 21.127708202361404 8 21.318991057413868 28.823040546150242 28.144072088978984 21.71389630745691 9 21.207474459810612 28.669048903935902 28.508949966609485 21.614526669644 10-11 21.282564499357857 29.133238761833542 28.028212089508465 21.555984649300143 12-13 21.24917864666602 29.14667291477943 27.886988189088807 21.717160249465746 14-15 21.419148214917172 29.147329659455774 27.72988648850861 21.703635637118445 16-17 21.629241949848723 28.951235672807357 27.65875940917162 21.760762968172305 18-19 21.54454695526022 28.982794309360028 27.656412187693302 21.81624654768645 20-21 21.687834627324325 28.90646559019432 27.674027103812044 21.731672678669312 22-23 21.626676185584063 28.95831584812683 27.658642335385125 21.75636563090398 24-25 21.59824597792832 28.892295416677754 27.613289646692348 21.89616895870158 26-27 21.762518160494366 28.800860267119376 27.695555762494894 21.741065809891357 28-29 21.79535669682007 28.906355896490744 27.614064342577173 21.68422306411202 30-31 21.757867293418705 28.911668179148947 27.60167673444245 21.7287877929899 32-33 21.7425687570887 28.998995358198265 27.487226756792825 21.77120912792021 34-35 21.780954435314715 29.063994942248822 27.453365585296304 21.701685037140162 36-37 21.825490577381593 28.962697062581388 27.46102210377278 21.750790256264242 38-39 21.823366657277678 29.014113015004828 27.439012668448065 21.723507659269426 40-41 21.82139420031292 29.10388956649349 27.379323751769785 21.6953924814238 42-43 21.81005736788896 29.063652829720837 27.384135008342426 21.74215479404778 44-45 21.781612874084814 29.09677064809851 27.38537412817236 21.736242349644318 46-47 21.812102087849 29.15894252205517 27.29275621394577 21.736199176150063 48-49 21.797488949400552 29.166484692850137 27.283917673025403 21.752108684723904 50-51 21.849619609550643 29.149594470994295 27.283183782489708 21.717602136965358 52-53 21.773666798201603 29.24393647733182 27.193879132510247 21.78851759195633 54-55 21.850493889599395 29.25327603717582 27.15316299627959 21.743067076945195 56-57 21.78813409278364 29.280436669531156 27.17476323362681 21.756666004058395 58-59 21.798176800559542 29.36464532083042 27.11893896094543 21.718238917664607 60-61 21.845845817120892 29.36237679428037 27.084836634885363 21.706940753713376 62-63 21.773601009493497 29.40443045728866 27.095458553868664 21.726509979349178 64-65 21.79852345949223 29.497607635506956 27.070285423264917 21.633583481735897 66-67 21.724576419637355 29.593062377766977 27.016862698035865 21.665498504559803 68-69 21.708175956595642 29.64155158209765 27.00228901487386 21.647983446432846 70-71 21.73791153594418 29.709203473290547 26.93464842534638 21.618236565418893 72-73 21.698778780560872 29.704229485138413 26.907121473412037 21.68987026088868 74-75 21.737901550533646 29.720139176440718 26.903561007054595 21.63839826597104 76-77 21.689815469400383 29.822923395193644 26.890185285610862 21.597075849795107 78-79 21.692726859636583 29.843268301604986 26.838636962313934 21.6253678764445 80-81 21.667995134492394 29.851821277847673 26.864739780038043 21.615443807621887 82-83 21.676053942439907 29.941037980611778 26.785531452887845 21.597376624060466 84-85 21.652516920909825 29.981673541642408 26.76039729800359 21.605412239444178 86-87 21.624967568141727 30.014484198502128 26.78900398933623 21.571544244019915 88-89 21.619841931475314 30.110033362375688 26.717190013004632 21.552934693144362 90-91 21.600535308296095 30.083516898053634 26.727935701711754 21.588012091938516 92-93 21.56585166176708 30.17202284796965 26.697102673009628 21.565022817253645 94-95 21.587731121748778 30.224676277713296 26.668954922657136 21.518637677880793 96-97 21.56338026010809 30.24861564830397 26.620200982610754 21.567803108977184 98-99 21.51521563580932 30.317198055748527 26.57962074196989 21.587965566472263 100-101 21.60202253875893 30.31940808785657 26.508955207143686 21.56961416624081 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 148281.0 1 83647.5 2 14520.5 3 9110.0 4 7791.0 5 6800.5 6 6261.5 7 6164.5 8 5679.0 9 5258.0 10 5106.0 11 5493.5 12 8551.5 13 8313.0 14 5589.5 15 5751.0 16 6239.0 17 7115.5 18 8206.5 19 10054.0 20 12760.0 21 16644.5 22 21344.5 23 26456.5 24 32562.0 25 40347.5 26 49798.5 27 60107.0 28 69748.0 29 78860.0 30 89582.5 31 102318.5 32 115684.5 33 129882.5 34 144449.5 35 157279.0 36 170628.5 37 183768.5 38 195080.0 39 202143.0 40 209854.0 41 221701.0 42 231360.0 43 239258.0 44 246700.5 45 254763.5 46 260586.5 47 262514.5 48 266932.0 49 271290.5 50 268840.5 51 259804.0 52 247159.0 53 236344.5 54 223062.0 55 206747.5 56 190169.0 57 171735.0 58 153854.0 59 134237.0 60 110692.0 61 87294.5 62 68290.5 63 52709.5 64 39128.5 65 27103.5 66 18224.5 67 12787.5 68 7866.5 69 4164.5 70 2228.5 71 996.0 72 442.5 73 227.5 74 121.0 75 68.5 76 50.5 77 42.0 78 33.0 79 23.5 80 13.5 81 8.5 82 7.5 83 7.0 84 5.0 85 4.5 86 3.5 87 1.5 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14465012541962674 2 0.0 3 0.0 4 0.0030646213012632787 5 3.6218251742202386E-4 6 0.0011004776490899954 7 0.0 8 2.368116460067079E-4 9 2.0895145235885992E-4 10-11 7.661553253158197E-5 12-13 0.0 14-15 6.965048411961996E-6 16-17 6.965048411961996E-6 18-19 6.965048411961996E-6 20-21 8.358058094354396E-5 22-23 1.8805630712297392E-4 24-25 0.0 26-27 6.965048411961996E-6 28-29 0.005655619310513142 30-31 0.0014556951181000574 32-33 3.2039222695025185E-4 34-35 2.298465975947459E-4 36-37 2.368116460067079E-4 38-39 0.0011004776490899954 40-41 0.0031690970274427083 42-43 4.6665824360145384E-4 44-45 4.387980499536058E-4 46-47 0.006296403764413645 48-49 0.006554110555656239 50-51 0.006749131911191175 52-53 0.008824716337955849 54-55 0.00453424651618726 56-57 0.0 58-59 2.7860193647847985E-5 60-61 7.940155189636676E-4 62-63 7.173999864320857E-4 64-65 4.0397280789379586E-4 66-67 6.338194054885417E-4 68-69 9.333164872029077E-4 70-71 0.0012606737625651216 72-73 0.002500452379894357 74-75 6.059592118406937E-4 76-77 0.0 78-79 0.0 80-81 6.965048411961997E-5 82-83 1.6716116188708792E-4 84-85 1.2537087141531594E-4 86-87 0.002625823251309673 88-89 0.0014696252149239814 90-91 4.736232920134158E-4 92-93 4.527281467775298E-4 94-95 2.368116460067079E-4 96-97 9.751067776746795E-4 98-99 0.0 100-101 6.965048411961996E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7178701.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.73365511853356 #Duplication Level Percentage of deduplicated Percentage of total 1 92.44340868560799 71.85964048748701 2 4.479133448529334 6.963588294357343 3 0.9985717588458642 2.3286789813969566 4 0.45637122292048793 1.4190161299409842 5 0.2917418897053129 1.1339081718991024 6 0.20247138020496874 0.9443304264135916 7 0.1457595401681378 0.7931295277966245 8 0.12016336729352951 0.7472590200861519 9 0.09822550158450692 0.687188453761353 >10 0.7466893804904866 10.353423116393245 >50 0.016364922509068066 0.8097401240728266 >100 7.657692714964906E-4 0.10326921766706317 >500 4.759040986345509E-5 0.019162770307686962 >1k 1.9036163929128476E-4 0.4966043019126465 >5k 4.759040982282119E-5 0.2978737961114891 >10k+ 4.759040982282119E-5 1.0431871803959119 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28199 0.3928148003378327 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 8052 0.11216513962623599 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.786019364784799E-5 2 0.0 0.0 0.0 0.0 2.786019364784799E-5 3 0.0 0.0 0.0 0.0 4.179029047177198E-5 4 2.786019364784799E-5 0.0 0.0 0.0 4.179029047177198E-5 5 2.786019364784799E-5 0.0 0.0 0.0 4.179029047177198E-5 6 2.786019364784799E-5 0.0 0.0 0.0 5.572038729569598E-5 7 2.786019364784799E-5 0.0 0.0 0.0 5.572038729569598E-5 8 2.786019364784799E-5 0.0 0.0 0.0 5.572038729569598E-5 9 2.786019364784799E-5 0.0 0.0 0.0 5.572038729569598E-5 10-11 4.179029047177198E-5 0.0 0.0 0.0 6.965048411961997E-5 12-13 6.965048411961997E-5 0.0 0.0 0.0 7.661553253158197E-5 14-15 7.661553253158197E-5 0.0 0.0 0.0 9.751067776746796E-5 16-17 9.751067776746796E-5 0.0 0.0 0.0 9.751067776746796E-5 18-19 1.2537087141531594E-4 0.0 0.0 6.965048411961997E-6 1.0447572617942996E-4 20-21 1.2537087141531594E-4 0.0 0.0 1.3930096823923994E-5 1.1840582300335395E-4 22-23 1.4626601665120194E-4 2.786019364784799E-5 0.0 1.3930096823923994E-5 1.2537087141531594E-4 24-25 1.6716116188708792E-4 2.786019364784799E-5 0.0 1.3930096823923994E-5 1.2537087141531594E-4 26-27 1.741262102990499E-4 3.482524205980999E-5 0.0 1.3930096823923994E-5 1.2537087141531594E-4 28-29 1.8109125871101193E-4 4.179029047177198E-5 0.0 1.3930096823923994E-5 1.2537087141531594E-4 30-31 2.1591650077082192E-4 4.179029047177198E-5 0.0 1.3930096823923994E-5 1.2537087141531594E-4 32-33 2.298465975947459E-4 4.179029047177198E-5 0.0 1.3930096823923994E-5 1.6716116188708792E-4 34-35 2.646718396545559E-4 4.179029047177198E-5 0.0 1.3930096823923994E-5 1.6716116188708792E-4 36-37 3.1342717853828986E-4 4.875533888373398E-5 0.0 1.3930096823923994E-5 1.8109125871101193E-4 38-39 3.4825242059809987E-4 5.572038729569598E-5 0.0 2.089514523588599E-5 1.8109125871101193E-4 40-41 3.8307766265790983E-4 5.572038729569598E-5 0.0 3.482524205980999E-5 1.8805630712297392E-4 42-43 4.3183300154164383E-4 6.268543570765798E-5 0.0 4.179029047177198E-5 1.950213555349359E-4 44-45 4.8755338883733977E-4 6.965048411961997E-5 0.0 4.179029047177198E-5 2.0895145235885992E-4 46-47 5.363087277210738E-4 8.358058094354396E-5 0.0 4.179029047177198E-5 2.0895145235885992E-4 48-49 6.407844539005037E-4 1.2537087141531594E-4 0.0 4.179029047177198E-5 2.0895145235885992E-4 50-51 7.034698896081617E-4 1.3930096823923995E-4 0.0 4.179029047177198E-5 2.0895145235885992E-4 52-53 7.870504705517056E-4 1.3930096823923995E-4 0.0 4.179029047177198E-5 2.228815491827839E-4 54-55 9.611766808507555E-4 1.3930096823923995E-4 0.0 4.179029047177198E-5 2.228815491827839E-4 56-57 0.0011422679395617676 1.5323106506316393E-4 0.0 4.179029047177198E-5 2.646718396545559E-4 58-59 0.0012954990046249314 1.5323106506316393E-4 0.0 4.875533888373398E-5 2.646718396545559E-4 60-61 0.0015741009411034113 1.6716116188708792E-4 0.0 5.572038729569598E-5 2.646718396545559E-4 62-63 0.0018109125871101192 1.6716116188708792E-4 0.0 5.572038729569598E-5 2.646718396545559E-4 64-65 0.0020895145235885993 1.6716116188708792E-4 0.0 5.572038729569598E-5 2.994970817143659E-4 66-67 0.0023750815084790412 1.741262102990499E-4 0.0 5.572038729569598E-5 3.1342717853828986E-4 68-69 0.002549207718778091 1.8805630712297392E-4 0.0 6.268543570765798E-5 3.2039222695025185E-4 70-71 0.002772089267960875 1.950213555349359E-4 0.0 6.965048411961997E-5 3.3432232377417583E-4 72-73 0.0030715863496752404 1.950213555349359E-4 0.0 6.965048411961997E-5 3.3432232377417583E-4 74-75 0.0034825242059809986 1.950213555349359E-4 0.0 6.965048411961997E-5 3.3432232377417583E-4 76-77 0.0038516717718149844 1.950213555349359E-4 0.0 6.965048411961997E-5 3.3432232377417583E-4 78-79 0.004436735838419793 1.950213555349359E-4 0.0 8.358058094354396E-5 3.3432232377417583E-4 80-81 0.005523283390685864 1.950213555349359E-4 0.0 9.751067776746796E-5 3.3432232377417583E-4 82-83 0.006756096959603137 1.950213555349359E-4 0.0 1.0447572617942996E-4 3.4825242059809987E-4 84-85 0.00851128915941756 2.0895145235885992E-4 0.0 1.1144077459139195E-4 3.4825242059809987E-4 86-87 0.010893335716308563 2.228815491827839E-4 0.0 1.1144077459139195E-4 3.6218251742202386E-4 88-89 0.01482162302065513 2.228815491827839E-4 0.0 1.1840582300335395E-4 3.6218251742202386E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 2205 0.0 25.670502 1 TATGCCG 1075 0.0 23.641674 48-49 ATCGGAA 2520 0.0 22.43024 2 CTCGTAT 1145 0.0 21.365967 44-45 TCTCGTA 1220 0.0 20.246746 42-43 ACACGTC 1385 0.0 19.890587 12-13 CGTCACG 265 1.8590072E-8 19.716843 3 CAACGCA 5170 0.0 19.56854 12-13 TCGGAAG 2925 0.0 19.162258 3 ATCAACG 5305 0.0 19.025799 10-11 CTCGCGT 170 8.931238E-10 18.16246 52-53 CGCGTAG 160 8.294592E-9 17.812437 54-55 TCAACGC 4945 0.0 17.337254 10-11 ATGCCGT 1510 0.0 17.14559 48-49 ACGCAGA 6150 0.0 16.759228 14-15 CGGAAGA 3480 0.0 16.379194 4 CGTACGC 160 1.6073864E-7 16.32744 20-21 TCGTATG 1630 0.0 16.174326 44-45 CGTATGC 1660 0.0 15.739375 46-47 GGTATCA 6865 0.0 15.705715 8 >>END_MODULE