FastQCFastQC Report
Sat 18 Jun 2016
SRR578549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7081581
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110753015.639586696812477No Hit
AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT245760.3470411480148289No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG98770.13947450435150005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97890.13823184399076985No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT86640.1223455609700715No Hit
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT85340.12050981270990192No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC73190.10335262704754772No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA73010.10309844651921654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2380650.091.687115
GGTATCA2392950.091.557338
TGGTATC2387950.091.511987
GTGGTAT2390350.091.490286
GTATCAA2397900.091.480089
AAGCAGT2399100.091.458631
AGCAGTG2409900.090.9476852
GCAGTGG2408600.090.8902053
CAGTGGT2411800.090.544354
TCAACGC2390600.046.50058712-13
AACGCAG2399150.046.3766314-15
CGCAGAG2403800.046.23890716-17
CAACGCA2390400.046.22421612-13
ATCAACG2390350.046.19465610-11
TATCAAC2401350.046.17202410-11
ACGCAGA2398550.046.09902214-15
CAGAGTA2384150.045.9485918-19
GAGTACT1397250.045.77105720-21
AGAGTAC2340550.045.714718-19
GCAGAGT2416800.045.54402516-17
Produced by FastQC (version 0.11.5)