##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR578549_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7081581 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.730059996489484 31.0 31.0 34.0 27.0 34.0 2 31.030736215542827 31.0 31.0 34.0 27.0 34.0 3 31.143127078543618 33.0 31.0 34.0 27.0 34.0 4 34.58767667841404 37.0 35.0 37.0 30.0 37.0 5 34.61819585202796 37.0 35.0 37.0 32.0 37.0 6 34.63547730372638 37.0 35.0 37.0 31.0 37.0 7 34.51195262752767 37.0 35.0 37.0 31.0 37.0 8 34.49620317835805 37.0 35.0 37.0 30.0 37.0 9 35.9500069264194 39.0 35.0 39.0 30.0 39.0 10-11 35.89481077742386 39.0 35.0 39.0 30.0 39.0 12-13 35.997426916390566 39.0 35.0 39.0 30.0 39.0 14-15 37.07905035612811 40.0 37.0 41.0 31.0 41.0 16-17 37.04181658022411 40.0 37.0 41.0 31.0 41.0 18-19 37.00791235742414 40.0 37.0 41.0 31.0 41.0 20-21 36.87065508394241 39.5 36.5 41.0 30.5 41.0 22-23 36.54886754808001 39.5 36.5 40.5 29.5 41.0 24-25 36.44943381993372 39.0 36.0 40.5 30.0 41.0 26-27 36.442042461986944 39.0 36.0 41.0 30.0 41.0 28-29 36.18224263762569 39.0 36.0 41.0 28.0 41.0 30-31 35.90906042026491 39.0 35.0 41.0 27.0 41.0 32-33 35.54985030885052 38.5 34.5 40.0 26.0 41.0 34-35 35.349985758829845 38.0 34.0 40.0 25.5 41.0 36-37 35.32248900351489 38.0 34.0 40.0 25.5 41.0 38-39 34.46487429855 37.0 33.5 40.0 25.0 41.0 40-41 34.40698418898266 37.0 33.0 40.0 25.0 41.0 42-43 34.206323630274085 37.0 33.0 40.0 24.0 41.0 44-45 34.040234235829544 37.0 33.0 40.0 23.5 41.0 46-47 33.814025709795594 36.0 33.0 40.0 23.0 41.0 48-49 33.391629496294684 36.0 33.0 40.0 21.0 41.0 50-51 31.99069607478895 35.5 31.5 39.0 15.0 40.5 52-53 31.416791956485422 35.5 31.0 39.0 5.0 40.0 54-55 30.856626295738195 36.0 29.5 40.0 2.0 41.0 56-57 30.186889128289287 36.0 28.0 40.0 2.0 41.0 58-59 29.797844224333524 35.5 27.0 40.0 2.0 41.0 60-61 29.482509202394212 35.0 26.5 39.0 2.0 41.0 62-63 29.145987527361477 35.0 26.0 39.0 2.0 41.0 64-65 28.821064745287813 35.0 26.0 39.0 2.0 40.5 66-67 28.518832023526947 35.0 26.0 38.0 2.0 40.0 68-69 28.193188004204146 35.0 25.5 38.0 2.0 40.0 70-71 27.82210667363686 34.5 24.5 37.0 2.0 40.0 72-73 27.446437666956008 34.0 24.0 36.5 2.0 39.0 74-75 27.060633988370675 34.0 22.5 36.0 2.0 39.0 76-77 26.671305743731523 34.0 21.5 35.5 2.0 38.5 78-79 26.297548386440823 34.0 20.0 35.0 2.0 37.5 80-81 25.929065275113004 33.0 18.5 35.0 2.0 37.0 82-83 25.57841073907084 33.0 17.5 35.0 2.0 36.5 84-85 25.194821467127184 33.0 13.0 35.0 2.0 36.0 86-87 24.85236870128295 33.0 7.5 35.0 2.0 36.0 88-89 24.479077341627526 32.0 4.5 35.0 2.0 35.0 90-91 24.123035449287386 32.0 2.0 35.0 2.0 35.0 92-93 23.76985471182212 32.0 2.0 35.0 2.0 35.0 94-95 23.345516488479056 31.5 2.0 35.0 2.0 35.0 96-97 22.88982968633699 31.0 2.0 35.0 2.0 35.0 98-99 22.316656548869524 31.0 2.0 34.0 2.0 35.0 100-101 20.786379129180332 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5083857957110922 1101 2 0.5450309228727015 1101 3 0.5751347295702445 1101 4 0.7175776413972628 1101 5 0.6752988242788334 1101 6 0.5508485328066683 1101 7 0.5615016029135376 1101 8 0.6429215846463521 1101 9 0.6060111322120605 1101 10-11 0.69635778626958 1101 12-13 0.6730832379060985 1101 14-15 0.8444316511084864 1101 16-17 0.7659918599577011 1101 18-19 0.7157813026393853 1101 20-21 0.7525365286974406 1101 22-23 0.8669343929672308 1101 24-25 0.9981108254160702 1101 26-27 0.9024583453047867 1101 28-29 0.8740082348145037 1101 30-31 0.969929717581131 1101 32-33 1.113097985919552 1101 34-35 1.060938528829105 1101 36-37 0.9768930750746492 1101 38-39 1.0289183869983347 1101 40-41 0.9965273044133838 1101 42-43 1.1983261245035806 1101 44-45 1.1205426789640498 1101 46-47 1.042981551631712 1101 48-49 1.036959298858143 1101 50-51 1.0855915663821314 1101 52-53 1.1327224857623612 1101 54-55 0.8413101749269458 1101 56-57 0.8495040817032198 1101 58-59 0.8764101262729653 1101 60-61 0.9602699839054232 1101 62-63 0.9050612374300506 1101 64-65 0.9422270513883539 1101 66-67 0.8386585601001748 1101 68-69 0.9078668118923865 1101 70-71 0.817995176852218 1101 72-73 0.9901888473419085 1101 74-75 0.9384483348900865 1101 76-77 0.8856380167261513 1101 78-79 0.8580360901430026 1101 80-81 1.055517440124035 1101 82-83 1.0538216223965442 1101 84-85 1.0810348292477308 1101 86-87 1.1171709648305814 1101 88-89 1.2118307121277034 1101 90-91 1.3672284037426934 1101 92-93 1.3515372565178723 1101 94-95 1.4067676065397698 1101 96-97 1.5971718318997432 1101 98-99 1.8368163545710345 1101 100-101 2.1603939251052076 1102 1 0.5125927928492082 1102 2 0.4750520173534021 1102 3 0.4560440349689223 1102 4 0.522982938028008 1102 5 0.43321479574339605 1102 6 0.4412981546779662 1102 7 0.5385832501903707 1102 8 0.5324682239219811 1102 9 0.5102616132029638 1102 10-11 0.5963500285869259 1102 12-13 0.6569754889435444 1102 14-15 0.8100408231391825 1102 16-17 0.7643217417055368 1102 18-19 0.6532948311921842 1102 20-21 0.7071545226132017 1102 22-23 0.758050883904879 1102 24-25 0.8085305306299588 1102 26-27 0.8195950079672016 1102 28-29 0.763777426256901 1102 30-31 0.8749889515712184 1102 32-33 0.9640601573007643 1102 34-35 1.0161296871706966 1102 36-37 0.8889642042753536 1102 38-39 0.8455071122856666 1102 40-41 0.8622550873289256 1102 42-43 1.010044512457121 1102 44-45 1.021485849855594 1102 46-47 1.0268425677889823 1102 48-49 1.0540190554710591 1102 50-51 0.9700795841163021 1102 52-53 1.0467770152720313 1102 54-55 0.9458715465085739 1102 56-57 0.8003189737708141 1102 58-59 0.8523366371035337 1102 60-61 0.8950078660988865 1102 62-63 0.9201650955939442 1102 64-65 0.9645381175476793 1102 66-67 0.88195582020915 1102 68-69 1.0795163897840396 1102 70-71 1.0061797303727893 1102 72-73 1.0304568781941548 1102 74-75 1.1574615693217112 1102 76-77 0.9737691413947367 1102 78-79 0.9687531537492902 1102 80-81 1.1189675849098073 1102 82-83 1.245519531234443 1102 84-85 1.3046202843510137 1102 86-87 1.2623221069452022 1102 88-89 1.4527889434227212 1102 90-91 1.3243642614726276 1102 92-93 1.3473447539973549 1102 94-95 1.4375242226619278 1102 96-97 1.6148126564422505 1102 98-99 1.774878053812678 1102 100-101 2.1361055831232214 1103 1 0.30151931130767196 1103 2 0.1906749404210153 1103 3 0.10769941434373465 1103 4 0.27508528565208223 1103 5 0.21141879098456684 1103 6 0.0760230328255389 1103 7 0.30349870496463893 1103 8 0.21836241854364857 1103 9 0.44788397439990746 1103 10-11 0.30658114996178654 1103 12-13 0.1941332043096935 1103 14-15 0.41830302491985805 1103 16-17 0.3819590062405993 1103 18-19 0.431069918445516 1103 20-21 0.5254303684179504 1103 22-23 0.5277425830561455 1103 24-25 0.39522612009380254 1103 26-27 0.5150882073833856 1103 28-29 0.4607618418786146 1103 30-31 0.4086427124330285 1103 32-33 0.6969820038694223 1103 34-35 0.5989665258610799 1103 36-37 0.5883984399288522 1103 38-39 0.41440648945185643 1103 40-41 0.4859702837311133 1103 42-43 0.35939251602822964 1103 44-45 0.47810749646286865 1103 46-47 0.6562951759781157 1103 48-49 0.6094631384811606 1103 50-51 0.5052453299269892 1103 52-53 0.24392680061927052 1103 54-55 0.30043649864488486 1103 56-57 0.33063954638327075 1103 58-59 0.42228362832287303 1103 60-61 0.31840954550257194 1103 62-63 0.35790672845149274 1103 64-65 0.4433106692123907 1103 66-67 0.4166155090238952 1103 68-69 0.4639605694486768 1103 70-71 0.5505386545318238 1103 72-73 0.5239628191043302 1103 74-75 0.6821194363201322 1103 76-77 0.6531748499346257 1103 78-79 0.7320597554064463 1103 80-81 0.63946634386593 1103 82-83 0.7708995300392196 1103 84-85 0.7558777620971107 1103 86-87 0.9397206501943351 1103 88-89 0.9558680582351009 1103 90-91 0.865228315130441 1103 92-93 0.9275392530928848 1103 94-95 0.9645381531200385 1103 96-97 1.138340679653755 1103 98-99 1.2791048924794666 1103 100-101 1.600872050957964 1104 1 0.1266944229808793 1104 2 0.22582632105927303 1104 3 0.17918830435465694 1104 4 0.18504829720654925 1104 5 0.048141512287244836 1104 6 0.07180269412164364 1104 7 0.2584409335254634 1104 8 0.18900570536624173 1104 9 0.3079038272147514 1104 10-11 0.35634908227464734 1104 12-13 0.2107415806711117 1104 14-15 0.3003132767773593 1104 16-17 0.4397007085011211 1104 18-19 0.5424480258255002 1104 20-21 0.43765057787783945 1104 22-23 0.3504736690378749 1104 24-25 0.44571122161739396 1104 26-27 0.5183523204729639 1104 28-29 0.6907201876885907 1104 30-31 0.5540567959071936 1104 32-33 0.6415970695980064 1104 34-35 0.6401421980716506 1104 36-37 0.7124173847955717 1104 38-39 0.5403218920518782 1104 40-41 0.580594048229095 1104 42-43 0.6571117193919562 1104 44-45 0.9778110092806571 1104 46-47 0.8379715264946839 1104 48-49 0.79812860145055 1104 50-51 0.7376626508914192 1104 52-53 0.6478058307528336 1104 54-55 0.680924938371863 1104 56-57 0.772204552651516 1104 58-59 0.8142436793256493 1104 60-61 0.7346406751806605 1104 62-63 0.6405643523581084 1104 64-65 0.749748235583823 1104 66-67 0.5193178877165643 1104 68-69 0.6490632720793776 1104 70-71 0.8379247818801865 1104 72-73 0.6774189846417791 1104 74-75 0.7911264382669785 1104 76-77 0.880010234026475 1104 78-79 0.954706670781416 1104 80-81 0.8285693913873153 1104 82-83 0.8474318162441214 1104 84-85 0.88172295352269 1104 86-87 0.9740960808014201 1104 88-89 1.0753564517906504 1104 90-91 1.1794628859750986 1104 92-93 1.1399722471427083 1104 94-95 1.1796800960660114 1104 96-97 1.31929453279459 1104 98-99 1.5278667826352006 1104 100-101 1.8591459757014164 1105 1 -0.04308483456662415 1105 2 0.06965782178718882 1105 3 0.08465820666559054 1105 4 0.1368499820859128 1105 5 0.11089845541621202 1105 6 0.14587580191349048 1105 7 0.27313147972219554 1105 8 0.2858402267691602 1105 9 0.23109219489277422 1105 10-11 0.2541185614395687 1105 12-13 0.16212014610863434 1105 14-15 0.06171680093067522 1105 16-17 0.21974394677737763 1105 18-19 0.18187851934883525 1105 20-21 0.16062338294683798 1105 22-23 0.27221960661567124 1105 24-25 0.3745633217211193 1105 26-27 0.35898075796419704 1105 28-29 0.4944648186599423 1105 30-31 0.32607894604790744 1105 32-33 0.521592131521885 1105 34-35 0.3996542778934895 1105 36-37 0.624571826845667 1105 38-39 0.4850353034185062 1105 40-41 0.4919577427601993 1105 42-43 0.6305095877832017 1105 44-45 0.7491919622815288 1105 46-47 0.5324744187141874 1105 48-49 0.6742977968000758 1105 50-51 0.6920523800473326 1105 52-53 0.4855242925775478 1105 54-55 0.7532036010211165 1105 56-57 0.8002497519887015 1105 58-59 0.7667564701914849 1105 60-61 0.676160862267853 1105 62-63 0.7686980845987215 1105 64-65 0.8546963100662346 1105 66-67 0.7931141320333701 1105 68-69 0.8372645911372807 1105 70-71 0.8690220151317583 1105 72-73 0.75502150617789 1105 74-75 0.7126060775146605 1105 76-77 0.6565934094804078 1105 78-79 0.7561968964282926 1105 80-81 0.7738529309180926 1105 82-83 0.9033237442874267 1105 84-85 0.9292636171537119 1105 86-87 0.9811871793612958 1105 88-89 0.9839660264687353 1105 90-91 1.2109988605592399 1105 92-93 1.276258498630174 1105 94-95 1.3010903932476872 1105 96-97 1.496962800882173 1105 98-99 1.7190875457220542 1105 100-101 2.0564557780747563 1106 1 0.22797397433171795 1106 2 0.28654502918606184 1106 3 0.1910938586437858 1106 4 0.02423901519717475 1106 5 0.25370551805156794 1106 6 0.2414838216327837 1106 7 0.375666836332087 1106 8 0.3424432105786721 1106 9 0.33065473685876157 1106 10-11 0.21128514706846602 1106 12-13 0.33939573114448507 1106 14-15 0.23178255142114068 1106 16-17 0.25393499912176054 1106 18-19 0.2525118670424149 1106 20-21 0.16334576088817698 1106 22-23 0.3589157850195761 1106 24-25 0.5436208473627246 1106 26-27 0.29518187451110833 1106 28-29 0.4506108106573876 1106 30-31 0.4511512567093021 1106 32-33 0.4536744256772778 1106 34-35 0.37841205952617685 1106 36-37 0.47742724572587747 1106 38-39 0.4835737671377629 1106 40-41 0.39984630668528354 1106 42-43 0.5674509359239082 1106 44-45 0.38431411344168254 1106 46-47 0.5099925101943086 1106 48-49 0.6892452124782196 1106 50-51 0.4872902813002007 1106 52-53 0.6007060085911142 1106 54-55 0.7259483390518149 1106 56-57 0.5630513218167899 1106 58-59 0.6839538243682988 1106 60-61 0.5239692661597566 1106 62-63 0.5837845093757821 1106 64-65 0.7983581872733403 1106 66-67 0.8173396651621623 1106 68-69 0.8716739391463584 1106 70-71 0.8602490067268747 1106 72-73 0.7780276909073116 1106 74-75 0.8064726616326077 1106 76-77 0.8607455848725216 1106 78-79 0.9389961661967234 1106 80-81 0.9337529391882704 1106 82-83 0.9566375751650931 1106 84-85 1.081556023288936 1106 86-87 1.1780046310977248 1106 88-89 1.1008624383774581 1106 90-91 1.0157849191509278 1106 92-93 1.1530509198659935 1106 94-95 1.2129959818142488 1106 96-97 1.3834899701054688 1106 98-99 1.5768264803021772 1106 100-101 1.8200206530600873 1107 1 0.03582472886487409 1107 2 0.10446215019473826 1107 3 0.13605101281567045 1107 4 0.03615620400848485 1107 5 0.1839545253652375 1107 6 0.19528905842775401 1107 7 0.2282881054171355 1107 8 0.21909482692048243 1107 9 0.1910683915651319 1107 10-11 0.208857385480826 1107 12-13 0.23303395424919415 1107 14-15 0.28925165687984133 1107 16-17 0.23175067330235777 1107 18-19 0.22198477774506387 1107 20-21 0.23248840732791365 1107 22-23 0.39510723712452744 1107 24-25 0.41951935740701884 1107 26-27 0.36001254530371796 1107 28-29 0.4338160699674134 1107 30-31 0.5170497832036105 1107 32-33 0.5062875162485767 1107 34-35 0.4003605780887156 1107 36-37 0.34964672091633275 1107 38-39 0.3786237404672903 1107 40-41 0.30669771979084004 1107 42-43 0.40500640462820314 1107 44-45 0.44178301327530534 1107 46-47 0.5075507571615319 1107 48-49 0.4056174848348135 1107 50-51 0.37795954656712993 1107 52-53 0.4126989461029815 1107 54-55 0.48851246904176193 1107 56-57 0.312533924433712 1107 58-59 0.32514476938393955 1107 60-61 0.43627061982357773 1107 62-63 0.5902379986294228 1107 64-65 0.539258008311581 1107 66-67 0.5757257082477274 1107 68-69 0.57784392685463 1107 70-71 0.5627068360642156 1107 72-73 0.568268374518059 1107 74-75 0.6023929779476198 1107 76-77 0.6318848972536948 1107 78-79 0.6621892635883384 1107 80-81 0.7094932645464986 1107 82-83 0.7704089996883887 1107 84-85 0.8414045702313189 1107 86-87 0.8578754045011827 1107 88-89 0.93754865837926 1107 90-91 0.9890273039783573 1107 92-93 1.0341222504526009 1107 94-95 1.143247465569008 1107 96-97 1.2181701566027598 1107 98-99 1.3607354941043042 1107 100-101 1.6334521703986091 1108 1 0.24329371758167184 1108 2 0.27305515400919944 1108 3 0.019012828139601368 1108 4 0.328552915303554 1108 5 0.3626455224032057 1108 6 0.306481439227575 1108 7 0.2858034340659401 1108 8 0.2007888017281516 1108 9 0.15871489511714287 1108 10-11 0.24482748835833945 1108 12-13 0.2890195824341646 1108 14-15 0.2629005582372841 1108 16-17 0.38911809675923337 1108 18-19 0.19222583478179445 1108 20-21 0.18051963958394168 1108 22-23 0.37637326480548694 1108 24-25 0.4956876171280342 1108 26-27 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-4.226463694178502 2316 96-97 -4.2434719045296525 2316 98-99 -4.406239278133064 2316 100-101 -4.595808988637618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72442.0 3 12490.0 4 10368.0 5 9728.0 6 11080.0 7 13568.0 8 17346.0 9 20573.0 10 24658.0 11 30267.0 12 31317.0 13 36411.0 14 42808.0 15 52459.0 16 67512.0 17 93396.0 18 138148.0 19 208899.0 20 275308.0 21 197377.0 22 108892.0 23 92326.0 24 96210.0 25 100748.0 26 108286.0 27 121602.0 28 139982.0 29 163902.0 30 194203.0 31 231673.0 32 281922.0 33 350342.0 34 453274.0 35 628865.0 36 914269.0 37 943645.0 38 679202.0 39 105985.0 40 98.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 9.653654712276726 51.86100945092187 23.00103706353375 15.484298773267655 2 15.429857472850289 53.84146151823504 16.20370553625098 14.524975472663682 3 49.00654378452146 19.170372351531466 17.201596654585405 14.621487209361664 4 15.42445084864445 17.419384749206028 18.123270374823797 49.03289402732573 5 15.172197770106512 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19.019621819232977 21.664476872874154 40.07894136568393 19.23695994220894 52-53 19.29130843570774 22.577228484420793 38.853738634974924 19.27772444489654 54-55 20.04268919914944 23.602156895239524 36.98909755490746 19.36605635070358 56-57 20.539237591660953 24.507450633451835 35.39959675750195 19.553715017385258 58-59 20.49412734580331 25.765885730048993 33.92534472460708 19.814642199540614 60-61 20.48878276389889 26.127058625407614 33.3674567104391 20.01670190025439 62-63 20.46263424389477 25.942428011701494 33.806271981014916 19.788665763388817 64-65 20.335415004065123 25.670643158619978 33.846633780681906 20.147308056632994 66-67 20.310809194710203 25.159214499653903 34.7033111431042 19.826665162531697 68-69 19.85637917897195 24.671358996884972 35.31195227529606 20.16030954884702 70-71 19.685991327850484 24.391543735862545 35.82408732620026 20.098377610086708 72-73 19.595042148378823 24.376311011133726 35.379165876395604 20.64948096409185 74-75 20.14321904339139 24.62921947090671 34.90249302461999 20.32506846108191 76-77 20.34691351065674 25.10246198371147 34.320833614113994 20.229790891517794 78-79 20.256799826056465 25.165463222852157 34.187547920299615 20.390189030791763 80-81 20.272189721131102 25.515785428514615 33.83700193593712 20.37502291441717 82-83 21.127181981475452 25.697446499823766 32.99218855399603 20.183182964704752 84-85 20.673681828036358 26.462041192544937 32.35496465045591 20.509312328962793 86-87 20.579980986939876 26.183712050969753 32.388049252543155 20.848257709547212 88-89 20.624288341002657 26.029410611603428 32.38989634423347 20.95640470316045 90-91 20.47094798847053 26.18856779779844 32.14190530110516 21.19857891262587 92-93 20.74727613922044 26.314258543866327 32.1950458717022 20.743419445211035 94-95 20.652592751711758 26.17991459696048 31.90073576079746 21.266756890530303 96-97 20.52690599033353 26.02795494160474 32.429464092303476 21.01567497575825 98-99 20.983294280926593 26.109444443463474 31.74665082204286 21.16061045356707 100-101 21.323547535034088 26.02047788608225 31.556565253725626 21.099409325158035 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11233.0 1 6768.0 2 1737.5 3 1104.0 4 942.5 5 782.0 6 827.0 7 1019.5 8 990.0 9 1020.0 10 1360.5 11 9238.0 12 174648.0 13 194682.0 14 51126.0 15 46892.0 16 50536.5 17 54262.0 18 56887.5 19 58173.5 20 58869.5 21 59576.0 22 60367.0 23 61673.0 24 63947.5 25 67784.0 26 72507.0 27 77630.5 28 83696.5 29 89535.0 30 95017.0 31 101055.0 32 107629.5 33 115264.0 34 127502.0 35 135795.5 36 139615.0 37 146755.0 38 154290.5 39 161783.5 40 170537.0 41 178707.0 42 184710.5 43 191014.5 44 199755.0 45 206259.5 46 209836.0 47 215327.0 48 218902.0 49 218775.0 50 216885.0 51 215845.0 52 213554.5 53 215055.0 54 208074.0 55 193259.5 56 194308.5 57 183341.5 58 159033.0 59 134636.0 60 108244.5 61 87423.5 62 68757.0 63 53145.5 64 39161.5 65 27874.0 66 19159.5 67 12575.5 68 8023.0 69 5062.5 70 3430.0 71 2530.0 72 2080.5 73 1759.0 74 1563.5 75 1450.0 76 1372.0 77 1311.5 78 1216.0 79 1141.0 80 1061.0 81 950.5 82 890.0 83 819.5 84 737.5 85 628.5 86 538.5 87 460.0 88 379.0 89 321.5 90 233.5 91 168.5 92 133.0 93 91.0 94 58.5 95 40.0 96 27.5 97 15.0 98 13.5 99 10.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12322107167876777 2 0.11072386236915174 3 0.10647339908983601 4 0.09475285250567635 5 0.09860792385203247 6 0.09041766238358355 7 0.09896095236360355 8 0.09510588101724742 9 0.09007875501247532 10-11 0.08068819660468474 12-13 0.07259678311947572 14-15 0.0802151383991795 16-17 0.07078221657000039 18-19 0.06307207387728814 20-21 0.05912521511792353 22-23 0.05248121853015591 24-25 0.04957932416504168 26-27 0.046197311024190786 28-29 0.048830903720511 30-31 0.03958861728758027 32-33 0.05224115914228758 34-35 0.0633191938353879 36-37 0.05291191331427262 38-39 0.05529132548226166 40-41 0.05548196087851004 42-43 0.050687833691374855 44-45 0.05049013772489505 46-47 0.050377168601192304 48-49 0.050257138907258136 50-51 0.05181752492840228 52-53 0.047955393011814736 54-55 0.048633207754031196 56-57 0.048830903720511 58-59 0.05152098097868259 60-61 0.04722815427797832 62-63 0.05683052979271155 64-65 0.05214231115904768 66-67 0.04681864120455587 68-69 0.04448865302818679 70-71 0.052968397876123986 72-73 0.04679039892363019 74-75 0.049028599686990804 76-77 0.0438037777157389 78-79 0.04832254266384865 80-81 0.05102674106248308 82-83 0.040471188566507964 84-85 0.051196194748037195 86-87 0.05386509029551452 88-89 0.049275719645090546 90-91 0.04516646777040325 92-93 0.042073938009040635 94-95 0.046056099619562356 96-97 0.03343180004578074 98-99 0.034151978209385724 100-101 0.025382749981960242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7081581.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.89794150767686 #Duplication Level Percentage of deduplicated Percentage of total 1 93.03316107574679 49.212627128591336 2 4.116057920747882 4.354619822678631 3 0.9572733819801883 1.5191337410053205 4 0.45785515657961384 0.9687838116694656 5 0.30673530658369125 0.8112833153001715 6 0.2089475570760219 0.6631737391427629 7 0.15645588626760884 0.5793336024221001 8 0.12362895197289724 0.5231773656094169 9 0.1009237554701756 0.4804793022236788 >10 0.515665375170751 4.3058427366897165 >50 0.00839490115260471 0.30831173818210095 >100 0.00858457144514319 1.130675699136117 >500 0.0027418988308642897 1.0198440530838642 >1k 0.0031335986637350192 3.3198027018669816 >5k 3.427373538460177E-4 1.481203310399454 >10k+ 9.792495824171935E-5 29.32170793199888 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1107530 15.639586696812477 No Hit AAGCAGTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT 24576 0.3470411480148289 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG 9877 0.13947450435150005 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9789 0.13823184399076985 No Hit TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT 8664 0.1223455609700715 No Hit AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT 8534 0.12050981270990192 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC 7319 0.10335262704754772 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA 7301 0.10309844651921654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4121140462842972E-5 0.0 0.0 0.0 0.0 2 1.4121140462842972E-5 0.0 0.0 0.0 0.0 3 1.4121140462842972E-5 0.0 0.0 0.0 0.0 4 1.4121140462842972E-5 0.0 0.0 0.0 0.0 5 1.4121140462842972E-5 0.0 0.0 0.0 0.0 6 2.8242280925685944E-5 0.0 0.0 0.0 0.0 7 2.8242280925685944E-5 1.4121140462842972E-5 0.0 0.0 0.0 8 2.8242280925685944E-5 1.4121140462842972E-5 0.0 0.0 0.0 9 4.2363421388528916E-5 1.4121140462842972E-5 0.0 0.0 0.0 10-11 5.648456185137189E-5 1.4121140462842972E-5 0.0 0.0 0.0 12-13 6.354513208279338E-5 2.8242280925685944E-5 0.0 7.060570231421486E-6 0.0 14-15 7.060570231421486E-5 2.8242280925685944E-5 0.0 1.4121140462842972E-5 0.0 16-17 7.060570231421486E-5 3.530285115710743E-5 0.0 1.4121140462842972E-5 7.060570231421486E-6 18-19 8.472684277705783E-5 4.2363421388528916E-5 0.0 1.4121140462842972E-5 2.1181710694264458E-5 20-21 9.178741300847933E-5 5.648456185137189E-5 0.0 2.8242280925685944E-5 2.8242280925685944E-5 22-23 9.88479832399008E-5 5.648456185137189E-5 0.0 2.8242280925685944E-5 2.8242280925685944E-5 24-25 9.88479832399008E-5 5.648456185137189E-5 0.0 2.8242280925685944E-5 2.8242280925685944E-5 26-27 1.1296912370274378E-4 5.648456185137189E-5 0.0 2.8242280925685944E-5 4.2363421388528916E-5 28-29 1.1296912370274378E-4 7.060570231421486E-5 0.0 2.8242280925685944E-5 4.2363421388528916E-5 30-31 1.2709026416558676E-4 7.060570231421486E-5 0.0 2.8242280925685944E-5 5.648456185137189E-5 32-33 1.4121140462842972E-4 7.060570231421486E-5 0.0 2.8242280925685944E-5 6.354513208279338E-5 34-35 1.4121140462842972E-4 9.178741300847933E-5 0.0 2.8242280925685944E-5 7.060570231421486E-5 36-37 1.8357482601695865E-4 9.88479832399008E-5 0.0 2.8242280925685944E-5 7.060570231421486E-5 38-39 2.2593824740548755E-4 9.88479832399008E-5 0.0 2.8242280925685944E-5 7.060570231421486E-5 40-41 2.75362239025438E-4 1.0590855347132228E-4 0.0 2.8242280925685944E-5 7.060570231421486E-5 42-43 3.247862306453884E-4 1.1296912370274378E-4 0.0 2.8242280925685944E-5 7.060570231421486E-5 44-45 3.883313627281817E-4 1.1296912370274378E-4 0.0 4.2363421388528916E-5 8.472684277705783E-5 46-47 4.94239916199504E-4 1.1296912370274378E-4 0.0 5.648456185137189E-5 8.472684277705783E-5 48-49 5.719061887451405E-4 1.1296912370274378E-4 0.0 5.648456185137189E-5 8.472684277705783E-5 50-51 7.201781636049916E-4 1.2709026416558676E-4 0.0 5.648456185137189E-5 8.472684277705783E-5 52-53 8.049050063820495E-4 1.2709026416558676E-4 0.0 5.648456185137189E-5 8.472684277705783E-5 54-55 8.755107086962644E-4 1.4121140462842975E-4 0.0 5.648456185137189E-5 9.178741300847933E-5 56-57 0.001030843253787537 1.6945368555411566E-4 0.0 7.060570231421486E-5 9.88479832399008E-5 58-59 0.001164994088184545 1.976959664798016E-4 0.0 7.766627254563634E-5 9.88479832399008E-5 60-61 0.0013062054928129748 1.976959664798016E-4 0.0 8.472684277705783E-5 9.88479832399008E-5 62-63 0.001525083169987041 1.976959664798016E-4 0.0 8.472684277705783E-5 9.88479832399008E-5 64-65 0.0017015974257725782 1.976959664798016E-4 0.0 8.472684277705783E-5 9.88479832399008E-5 66-67 0.0018922328220209582 2.188776771740661E-4 0.0 8.472684277705783E-5 9.88479832399008E-5 68-69 0.002132292209889289 2.2593824740548755E-4 0.0 8.472684277705783E-5 9.88479832399008E-5 70-71 0.002428836159608991 2.2593824740548755E-4 0.0 8.472684277705783E-5 9.88479832399008E-5 72-73 0.002774804100948644 2.2593824740548755E-4 0.0 8.472684277705783E-5 9.88479832399008E-5 74-75 0.0030784086208997677 2.2593824740548755E-4 0.0 8.472684277705783E-5 9.88479832399008E-5 76-77 0.003572648537099272 2.2593824740548755E-4 0.0 8.472684277705783E-5 9.88479832399008E-5 78-79 0.004052767312835933 2.2593824740548755E-4 0.0 9.88479832399008E-5 9.88479832399008E-5 80-81 0.004914156881069354 2.2593824740548755E-4 0.0 9.88479832399008E-5 9.88479832399008E-5 82-83 0.006107393250179585 2.2593824740548755E-4 0.0 9.88479832399008E-5 9.88479832399008E-5 84-85 0.007787808965257899 2.2593824740548755E-4 0.0 9.88479832399008E-5 9.88479832399008E-5 86-87 0.010040130869081353 2.2593824740548755E-4 0.0 9.88479832399008E-5 1.0590855347132228E-4 88-89 0.014043474190297337 2.2593824740548755E-4 0.0 9.88479832399008E-5 1.1296912370274378E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGGTA 238065 0.0 91.68711 5 GGTATCA 239295 0.0 91.55733 8 TGGTATC 238795 0.0 91.51198 7 GTGGTAT 239035 0.0 91.49028 6 GTATCAA 239790 0.0 91.48008 9 AAGCAGT 239910 0.0 91.45863 1 AGCAGTG 240990 0.0 90.947685 2 GCAGTGG 240860 0.0 90.890205 3 CAGTGGT 241180 0.0 90.54435 4 TCAACGC 239060 0.0 46.500587 12-13 AACGCAG 239915 0.0 46.37663 14-15 CGCAGAG 240380 0.0 46.238907 16-17 CAACGCA 239040 0.0 46.224216 12-13 ATCAACG 239035 0.0 46.194656 10-11 TATCAAC 240135 0.0 46.172024 10-11 ACGCAGA 239855 0.0 46.099022 14-15 CAGAGTA 238415 0.0 45.94859 18-19 GAGTACT 139725 0.0 45.771057 20-21 AGAGTAC 234055 0.0 45.7147 18-19 GCAGAGT 241680 0.0 45.544025 16-17 >>END_MODULE